RLG00000032303

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000925
Physical Location & Seq
Reverse (-)
13323590 .. 13325221
1632 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032303

Sequence Viewer

Length: 1632 bp
ATGAATATGGAGACCTTTCATGTAAAACTGTTTTCCCTACTTTTCATCCTTCTCAACTCAGTTTGGTCCACAACTTCAACATCAAATCCTGAAAGCTTTGTTCAATGCTTTTCCTCCCACATACAGGACTCTAACTCCAGTAATGAAATCATTCTCACCAGAACTAGTTCTGCTTATCCCTCAGTCTTAGATTTTTCCATACACAACTTCAGATTCTTGAACAATTCCACACCAAAACCAGAGGCCATCATTACTCCTTTTGATGAATCTCAGGTTCAAGCAGCTGTAATTTGCTCCAAGAAAAGTGGCATACAATTAAGAATCCGAAGCGGGGGGCATGATTACGAGGGCCTATCTTATGTATCCAGAGCTCCTTTCATCATAATTGACCTTTTCAATCTCCGTTCCATCGATGTTGATATAAAGAATGAGAGTGCTTGGGTCAAATCAGGCGCTACTCTCGGAGAGTTGTACTACAACATCGCACAGAAGAGTAAATTCTTTGGCTTTCCAGCAGGGACTTGTCCCACCGTCGGTGTTGGGGGACACATCAGTGGGGGTGGAGTTGGAGCCATAGTCAGAAAATATGGTCTAGCATCCGACAATGTCATTGATGCTAGAATTGTAGATGTTAACGGCAGAATTCTAGACAGAAAGTCCATGGGAGAAGAGCTCTTTTGGGCTATCAGAGGAGGAGGAGGATCAAGCTTTGGAGTCATTCTAGCATGGAAACTTAGACTGGTTCCTGTTCCCCCAACTGTAACAGTTTGCGACATTACAAAGACAAAAGAACAAGGTGCAACCAAACTTCTTTTGAAATGGCAAACCATTGCAGACACGCTTCCTGAAGACCTTTACCTACATTCAATTGTAGGAAGTAGCAACAACACTATAACAATTGCATTCGGTTCCTTGTTTCTTGGGAGGGTCGACAAAGCTGTCCAGGTGATACAGGAAAACTTCCCAGAGTTGAGTTTAGAAAGCAGCCATTGCAGTGAGATGAGTTGGATTCAATCGGTTTTGAACTCTTCTGGTTACTCAATGCAAGAACCCTTAGAGATTTTGCTGAACCGCCGGCAACTCAGCTACTACTACTTCTTCAAAGCAAAATCAGACTTTGTGACTGAACCCATCTCAGAAGCTGGCTTGGAAGGCTTATGGAACATACTGCTTGAAGCAAACACATCCTATATGATATTGACACCATATGGAGGAAAAATGAGTGAGATTTCCGATTCAGAAATTGCTTTCCCACATCGAAAAGGAAACAAATATGAAATCCAATATATGGTTACTTGGGGTGATGACAAGGACACGAAGAAGTATGTTGGATTTATGAGAAGGTTGTATGCATACATGGCACCATATGTTTCAAAGTCTCCTAGAGCTGCCTATTTGAATTATAGGGATCTTGATTTGGGGAGGAACAGTTATGGCAATACAAGCTATGCACAAGCAAGCATTTGGGGTTTGAAGTACTTCAAGAACAACTTTAGGAGACTTGTTCATGTTAAGACCTTGGTTGATCCTGGCAACTTCTTTAGGAATGAGCAAAGCATCCCCGTGTTTTCGTCAGGAAAGTACAAGGTCAACAAATATATGGGCTTGGCTTCCAGGATAGATAATAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

544

Amino Acids

60.82

Weight (kDa)

8.86

Isoelectric Point (pI)

43.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 80 - 216 6.8e-27 FAD binding domain
BBE PF08031 464 - 521 2.3e-18 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 938
AccB1I GGYRCC 1 cut(s) 1360
AccB7I CCANNNNNTGG 1 cut(s) 1288
AccI GTMKAC 1 cut(s) 930
AciI CCGC 2 cut(s) 330, 1072
AclWI GGATC 3 cut(s) 709, 1416, 1520
AcsI RAATTY 2 cut(s) 497, 642
AcuI CTGAAG 2 cut(s) 193, 867
AfaI GTAC 3 cut(s) 473, 1478, 1583
AfiI CCNNNNNNNGG 5 cut(s) 124, 331, 533, 1211, 1288
AhdI GACNNNNNGTC 1 cut(s) 655
AhlI ACTAGT 1 cut(s) 164
AjnI CCWGG 3 cut(s) 942, 1528, 1613
AleI CACNNNNGTG 1 cut(s) 552
Alw21I GWGCWC 2 cut(s) 373, 675
Alw26I GTCTC 3 cut(s) 5, 1383, 1492
AlwI GGATC 3 cut(s) 709, 1416, 1520
AlwNI CAGNNNCTG 1 cut(s) 1142
AoxI GGCC 2 cut(s) 243, 349
ApeKI GCWGC 3 cut(s) 281, 984, 1388
ApoI RAATTY 2 cut(s) 497, 642
Asp700I GAANNNNTTC 3 cut(s) 150, 166, 1478
AspLEI GCGC 1 cut(s) 455
AspS9I GGNCC 2 cut(s) 66, 349
AsuHPI GGTGA 3 cut(s) 148, 958, 1313
AvaII GGWCC 1 cut(s) 66
BanI GGYRCC 1 cut(s) 1360
BanII GRGCYC 2 cut(s) 373, 675
BbsI GAAGAC 1 cut(s) 855
Bbv12I GWGCWC 2 cut(s) 373, 675
BbvI GCAGC 3 cut(s) 293, 996, 1375
BccI CCATC 3 cut(s) 254, 416, 1139
BceAI ACGGC 1 cut(s) 652
BciT130I CCWGG 3 cut(s) 944, 1530, 1615
BciVI GTATCC 1 cut(s) 373
BcoDI GTCTC 3 cut(s) 5, 1383, 1492
BcuI ACTAGT 1 cut(s) 164
BfaI CTAG 6 cut(s) 165, 593, 618, 647, 722, 1383
BfoI RGCGCY 1 cut(s) 456
BfuI GTATCC 1 cut(s) 373
BisI GCNGC 3 cut(s) 282, 985, 1389
BlsI GCNGC 3 cut(s) 283, 986, 1390
BmcAI AGTACT 1 cut(s) 1478
Bme1390I CCNGG 3 cut(s) 944, 1530, 1615
Bme18I GGWCC 1 cut(s) 66
BmeRI GACNNNNNGTC 1 cut(s) 655
BmgT120I GGNCC 2 cut(s) 66, 349
BmiI GGNNCC 4 cut(s) 571, 744, 910, 1362
BmrFI CCNGG 3 cut(s) 944, 1530, 1615
BmsI GCATC 3 cut(s) 604, 605, 1566
BpiI GAAGAC 1 cut(s) 855
BplI GAGNNNNNCTC 2 cut(s) 657, 689
BpmI CTGGAG 1 cut(s) 121
Bsa29I ATCGAT 1 cut(s) 411
BsaI GGTCTC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 660, 1518
BsaXI ACNNNNNCTCC 6 cut(s) 119, 149, 561, 591, 1414, 1444
Bsc4I CCNNNNNNNGG 5 cut(s) 124, 331, 533, 1211, 1288
Bse118I RCCGGY 1 cut(s) 1074
Bse1I ACTGG 2 cut(s) 138, 744
Bse3DI GCAATG 2 cut(s) 828, 988
BseBI CCWGG 3 cut(s) 944, 1530, 1615
BseCI ATCGAT 1 cut(s) 411
BseDI CCNNGG 2 cut(s) 660, 1518
BseGI GGATG 4 cut(s) 45, 596, 1184, 1557
BseLI CCNNNNNNNGG 5 cut(s) 124, 331, 533, 1211, 1288
BseMI GCAATG 2 cut(s) 828, 988
BseMII CTCAG 5 cut(s) 72, 195, 284, 1096, 1149
BseNI ACTGG 2 cut(s) 138, 744
BseRI GAGGAG 3 cut(s) 705, 708, 711
BseXI GCAGC 3 cut(s) 293, 996, 1375
BshFI GGCC 2 cut(s) 245, 351
BshNI GGYRCC 1 cut(s) 1360
BshVI ATCGAT 1 cut(s) 411
BsiHKAI GWGCWC 2 cut(s) 373, 675
BsiSI CCGG 1 cut(s) 1075
BslFI GGGAC 3 cut(s) 510, 532, 558
BslI CCNNNNNNNGG 5 cut(s) 124, 331, 533, 1211, 1288
BsmAI GTCTC 3 cut(s) 5, 1383, 1492
BsmFI GGGAC 3 cut(s) 510, 532, 558
BsmI GAATGC 1 cut(s) 902
BsnI GGCC 2 cut(s) 245, 351
Bso31I GGTCTC 1 cut(s) 5
Bsp1286I GDGCHC 2 cut(s) 373, 675
Bsp143I GATC 3 cut(s) 701, 1408, 1525
Bsp19I CCATGG 1 cut(s) 660
BspACI CCGC 2 cut(s) 330, 1072
BspANI GGCC 2 cut(s) 245, 351
BspCNI CTCAG 5 cut(s) 71, 194, 283, 1095, 1148
BspDI ATCGAT 1 cut(s) 411
BspLI GGNNCC 4 cut(s) 571, 744, 910, 1362
BspPI GGATC 3 cut(s) 709, 1416, 1520
BspQI GCTCTTC 1 cut(s) 663
BspT107I GGYRCC 1 cut(s) 1360
BspTNI GGTCTC 1 cut(s) 5
BsrDI GCAATG 2 cut(s) 828, 988
BsrFI RCCGGY 1 cut(s) 1074
BsrI ACTGG 2 cut(s) 138, 744
BssAI RCCGGY 1 cut(s) 1074
BssECI CCNNGG 2 cut(s) 660, 1518
BssMI GATC 3 cut(s) 701, 1408, 1525
BssT1I CCWWGG 2 cut(s) 660, 1518
Bst2UI CCWGG 3 cut(s) 944, 1530, 1615
Bst4CI ACNGT 5 cut(s) 30, 532, 760, 766, 1430
Bst6I CTCTTC 3 cut(s) 485, 663, 1033
BstAPI GCANNNNNTGC 1 cut(s) 990
BstC8I GCNNGC 3 cut(s) 1076, 1144, 1459
BstDEI CTNAG 8 cut(s) 58, 181, 187, 270, 734, 1054, 1082, 1135
BstDSI CCRYGG 1 cut(s) 660
BstF5I GGATG 4 cut(s) 45, 596, 1184, 1557
BstH2I RGCGCY 1 cut(s) 456
BstHHI GCGC 1 cut(s) 455
BstKTI GATC 3 cut(s) 704, 1411, 1528
BstMAI GTCTC 3 cut(s) 5, 1383, 1492
BstMBI GATC 3 cut(s) 701, 1408, 1525
BstMWI GCNNNNNNNGC 4 cut(s) 990, 1152, 1358, 1443
BstNI CCWGG 3 cut(s) 944, 1530, 1615
BstSCI CCNGG 3 cut(s) 942, 1528, 1613
BstV1I GCAGC 3 cut(s) 293, 996, 1375
BstV2I GAAGAC 1 cut(s) 855
BstX2I RGATCY 1 cut(s) 1408
BstYI RGATCY 1 cut(s) 1408
Bsu15I ATCGAT 1 cut(s) 411
BsuI GTATCC 1 cut(s) 373
BsuRI GGCC 2 cut(s) 245, 351
BsuTUI ATCGAT 1 cut(s) 411
BtgI CCRYGG 1 cut(s) 660
BtgZI GCGATG 1 cut(s) 466
BtsCI GGATG 4 cut(s) 45, 596, 1184, 1557
BtsI GCAGTG 1 cut(s) 1000
BtsIMutI CAGTG 2 cut(s) 559, 1000
Cac8I GCNNGC 3 cut(s) 1076, 1144, 1459
CaiI CAGNNNCTG 1 cut(s) 1142
CfoI GCGC 1 cut(s) 455
Cfr10I RCCGGY 1 cut(s) 1074
Cfr13I GGNCC 2 cut(s) 66, 349
ClaI ATCGAT 1 cut(s) 411
Csp6I GTAC 3 cut(s) 472, 1477, 1582
CspCI CAANNNNNGTGG 2 cut(s) 286, 321
CviAII CATG 6 cut(s) 20, 338, 661, 726, 1357, 1508
CviQI GTAC 3 cut(s) 472, 1477, 1582
DdeI CTNAG 8 cut(s) 58, 181, 187, 270, 734, 1054, 1082, 1135
DpnI GATC 3 cut(s) 703, 1410, 1527
DpnII GATC 3 cut(s) 701, 1408, 1525
DrdI GACNNNNNNGTC 1 cut(s) 938
DriI GACNNNNNGTC 1 cut(s) 655
DseDI GACNNNNNNGTC 1 cut(s) 938
Eam1104I CTCTTC 3 cut(s) 485, 663, 1033
Eam1105I GACNNNNNGTC 1 cut(s) 655
EarI CTCTTC 3 cut(s) 485, 663, 1033
Ecl136II GAGCTC 2 cut(s) 371, 673
Eco130I CCWWGG 2 cut(s) 660, 1518
Eco24I GRGCYC 2 cut(s) 373, 675
Eco31I GGTCTC 1 cut(s) 5
Eco47I GGWCC 1 cut(s) 66
Eco53kI GAGCTC 2 cut(s) 371, 673
Eco57I CTGAAG 2 cut(s) 193, 867
EcoICRI GAGCTC 2 cut(s) 371, 673
EcoO109I RGGNCCY 1 cut(s) 349
EcoRI GAATTC 1 cut(s) 642
EcoRII CCWGG 3 cut(s) 942, 1528, 1613
EcoT14I CCWWGG 2 cut(s) 660, 1518
EcoT22I ATGCAT 1 cut(s) 1354
EcoT38I GRGCYC 2 cut(s) 373, 675
ErhI CCWWGG 2 cut(s) 660, 1518
FaeI CATG 6 cut(s) 23, 341, 664, 729, 1360, 1511
FalI AAGNNNNNCTT 2 cut(s) 1475, 1507
FaqI GGGAC 3 cut(s) 510, 532, 558
FatI CATG 6 cut(s) 19, 337, 660, 725, 1356, 1507
FauI CCCGC 1 cut(s) 323
FauNDI CATATG 2 cut(s) 1207, 1366
FblI GTMKAC 1 cut(s) 930
Fnu4HI GCNGC 3 cut(s) 282, 985, 1389
FokI GGATG 4 cut(s) 32, 583, 1171, 1544
FriOI GRGCYC 2 cut(s) 373, 675
Fsp4HI GCNGC 3 cut(s) 282, 985, 1389
FspBI CTAG 6 cut(s) 165, 593, 618, 647, 722, 1383
GlaI GCGC 1 cut(s) 454
GluI GCNGC 3 cut(s) 282, 985, 1389
GsuI CTGGAG 1 cut(s) 121
HaeII RGCGCY 1 cut(s) 456
HaeIII GGCC 2 cut(s) 245, 351
HapII CCGG 1 cut(s) 1075
HhaI GCGC 1 cut(s) 455
Hin1II CATG 6 cut(s) 23, 341, 664, 729, 1360, 1511
Hin6I GCGC 1 cut(s) 453
HinP1I GCGC 1 cut(s) 453
HincII GTYRAC 3 cut(s) 634, 931, 1591
HindII GTYRAC 3 cut(s) 634, 931, 1591
HindIII AAGCTT 2 cut(s) 94, 706
HinfI GANTC 7 cut(s) 128, 213, 266, 321, 714, 1009, 1235
HpaI GTTAAC 1 cut(s) 634
HpaII CCGG 1 cut(s) 1075
HphI GGTGA 3 cut(s) 148, 958, 1313
Hpy166II GTNNAC 4 cut(s) 69, 634, 931, 1591
Hpy188III TCNNGA 8 cut(s) 89, 217, 366, 647, 845, 1412, 1483, 1575
Hpy8I GTNNAC 4 cut(s) 69, 634, 931, 1591
Hpy99I CGWCG 1 cut(s) 536
HpyAV CCTTC 3 cut(s) 59, 1145, 1335
HpyCH4III ACNGT 5 cut(s) 30, 532, 760, 766, 1430
HpyCH4V TGCA 7 cut(s) 800, 833, 902, 993, 1045, 1352, 1451
HpyF10VI GCNNNNNNNGC 4 cut(s) 990, 1152, 1358, 1443
HpyF3I CTNAG 8 cut(s) 58, 181, 187, 270, 734, 1054, 1082, 1135
Hsp92II CATG 6 cut(s) 23, 341, 664, 729, 1360, 1511
HspAI GCGC 1 cut(s) 453
KroI GCCGGC 1 cut(s) 1074
KroNI GCCGGC 1 cut(s) 1076
KspAI GTTAAC 1 cut(s) 634
Kzo9I GATC 3 cut(s) 701, 1408, 1525
LguI GCTCTTC 1 cut(s) 663
LmnI GCTCC 3 cut(s) 299, 376, 569
Lsp1109I GCAGC 3 cut(s) 293, 996, 1375
LweI GCATC 3 cut(s) 604, 605, 1566
MaeI CTAG 6 cut(s) 165, 593, 618, 647, 722, 1383
MaeIII GTNAC 4 cut(s) 760, 1034, 1120, 1291
MalI GATC 3 cut(s) 703, 1410, 1527
MboI GATC 3 cut(s) 701, 1408, 1525
MboII GAAGA 6 cut(s) 502, 680, 860, 1020, 1090, 1330
MfeI CAATTG 2 cut(s) 867, 897
MflI RGATCY 1 cut(s) 1408
MhlI GDGCHC 2 cut(s) 373, 675
MlyI GAGTC 2 cut(s) 122, 723
MmeI TCCRAC 4 cut(s) 547, 624, 986, 1309
Mph1103I ATGCAT 1 cut(s) 1354
MroNI GCCGGC 1 cut(s) 1074
MroXI GAANNNNTTC 3 cut(s) 150, 166, 1478
MseI TTAA 3 cut(s) 317, 633, 1512
MslI CAYNNNNRTG 3 cut(s) 552, 993, 1562
MspA1I CMGCKG 1 cut(s) 284
MspI CCGG 1 cut(s) 1075
MspR9I CCNGG 3 cut(s) 944, 1530, 1615
MunI CAATTG 2 cut(s) 867, 897
Mva1269I GAATGC 1 cut(s) 902
MvaI CCWGG 3 cut(s) 944, 1530, 1615
MwoI GCNNNNNNNGC 4 cut(s) 990, 1152, 1358, 1443
NaeI GCCGGC 1 cut(s) 1076
NcoI CCATGG 1 cut(s) 660
NdeI CATATG 2 cut(s) 1207, 1366
NdeII GATC 3 cut(s) 701, 1408, 1525
NgoMIV GCCGGC 1 cut(s) 1074
NlaIII CATG 6 cut(s) 23, 341, 664, 729, 1360, 1511
NlaIV GGNNCC 4 cut(s) 571, 744, 910, 1362
NmuCI GTSAC 1 cut(s) 1120
NsiI ATGCAT 1 cut(s) 1354
OliI CACNNNNGTG 1 cut(s) 552
PciSI GCTCTTC 1 cut(s) 663
PctI GAATGC 1 cut(s) 902
PdiI GCCGGC 1 cut(s) 1076
PdmI GAANNNNTTC 3 cut(s) 150, 166, 1478
PfeI GAWTC 5 cut(s) 213, 266, 321, 1009, 1235
PflFI GACNNNGTC 1 cut(s) 605
PflMI CCANNNNNTGG 1 cut(s) 1288
PfoI TCCNGGA 1 cut(s) 1613
PkrI GCNGC 3 cut(s) 283, 986, 1390
PleI GAGTC 2 cut(s) 122, 722
PpsI GAGTC 2 cut(s) 122, 722
Psp124BI GAGCTC 2 cut(s) 373, 675
Psp6I CCWGG 3 cut(s) 942, 1528, 1613
PspGI CCWGG 3 cut(s) 942, 1528, 1613
PspN4I GGNNCC 4 cut(s) 571, 744, 910, 1362
PspPI GGNCC 2 cut(s) 66, 349
PstNI CAGNNNCTG 1 cut(s) 1142
PsuI RGATCY 1 cut(s) 1408
PsyI GACNNNGTC 1 cut(s) 605
PvuII CAGCTG 1 cut(s) 284
RsaI GTAC 3 cut(s) 473, 1478, 1583
RsaNI GTAC 3 cut(s) 472, 1477, 1582
RseI CAYNNNNRTG 3 cut(s) 552, 993, 1562
SacI GAGCTC 2 cut(s) 373, 675
SalI GTCGAC 1 cut(s) 929
SapI GCTCTTC 1 cut(s) 663
SaqAI TTAA 3 cut(s) 317, 633, 1512
SatI GCNGC 3 cut(s) 282, 985, 1389
Sau3AI GATC 3 cut(s) 701, 1408, 1525
Sau96I GGNCC 2 cut(s) 66, 349
ScaI AGTACT 1 cut(s) 1478
SchI GAGTC 2 cut(s) 122, 723
ScrFI CCNGG 3 cut(s) 944, 1530, 1615
SduI GDGCHC 2 cut(s) 373, 675
SfaNI GCATC 3 cut(s) 604, 605, 1566
SinI GGWCC 1 cut(s) 66
SmiMI CAYNNNNRTG 3 cut(s) 552, 993, 1562
SpeI ACTAGT 1 cut(s) 164
SsiI CCGC 2 cut(s) 330, 1072
SspMI CTAG 6 cut(s) 165, 593, 618, 647, 722, 1383
SstI GAGCTC 2 cut(s) 373, 675
StyD4I CCNGG 3 cut(s) 942, 1528, 1613
StyI CCWWGG 2 cut(s) 660, 1518
TaaI ACNGT 5 cut(s) 30, 532, 760, 766, 1430
TaqI TCGA 3 cut(s) 411, 930, 1258
TatI WGTACW 3 cut(s) 471, 1476, 1581
TfiI GAWTC 5 cut(s) 213, 266, 321, 1009, 1235
Tru1I TTAA 3 cut(s) 317, 633, 1512
Tru9I TTAA 3 cut(s) 317, 633, 1512
TscAI CASTG 2 cut(s) 559, 1000
TseFI GTSAC 1 cut(s) 1120
TseI GCWGC 3 cut(s) 281, 984, 1388
Tsp45I GTSAC 1 cut(s) 1120
TspDTI ATGAA 8 cut(s) 8, 17, 34, 159, 279, 367, 1290, 1496
TspGWI ACGGA 1 cut(s) 392
TspRI CASTG 2 cut(s) 559, 1000
Tth111I GACNNNGTC 1 cut(s) 605
Van91I CCANNNNNTGG 1 cut(s) 1288
VpaK11BI GGWCC 1 cut(s) 66
XapI RAATTY 2 cut(s) 497, 642
XbaI TCTAGA 1 cut(s) 646
XmiI GTMKAC 1 cut(s) 930
XmnI GAANNNNTTC 3 cut(s) 150, 166, 1478
XspI CTAG 6 cut(s) 165, 593, 618, 647, 722, 1383
ZrmI AGTACT 1 cut(s) 1478
Zsp2I ATGCAT 1 cut(s) 1354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.