pycom10g20490

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000925
Physical Location & Seq
Forward (+)
23352792 .. 23354568
1777 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g20490.3

Sequence Viewer

Length: 1653 bp
ATGAAAAAATTCGCATTGAAAATGTCTCCTGTTAGGCAAACGTTTATGAAAAGCTTCTTCTCTCAAAGCTCATACCATTTCACATTTATAGAACAAATAAAGATGGAGATCTCACACATAAAACTACTTCCCCTACTACTTTTCTTCCTTCTAAACTCAGTTTGTTTTACAAATTCAAACCCGATTTCGGAAAGCTTTGTCCCATGCTTTTCATCCCGCATAAATTCTACCTCAACAAGAAAAATCATCCTCACCGAAAGCACTTCGGACTATTCCTTAGTCTTAAAATCTTTCATACAGAATCTCCGATTCTTGAACACATCAACACCGAAACCACAAGCTATCATTACTCCTTCTCAATACTCTCATGTTCAGGCAGCTGTGGTATGTGCCAAGAAAAACGGCATACAAATAAGAACCCGAAGCGGGGGCCATGACTACGAGGGCCTATCTTATTTTGATCGATCTATCGATGTTGACATAGAGAATGAGAGTGCTCGAGTTGAGTCAGGTGCTACTCTCGGAGAGTTGTACTACACAATTTCACAAAAGAGTAAAGTTCATGGATTTCCAGCAGGGTCATGCCCAACCATCGGTGTAGGGGGACATATTAGCGGAGGTGGATTTGGTACTATACTCCGAAAATATGGTCTAGCAGCTGACAGTGTTATTGATGCTAAGATTGTTGACGTTAATGGCAGGATTCTCGACAGAAAATCCATGGGAGAAGAACTCTTTTGGGCTATTCGAGGAGGGGGAGGCTCAAGCTTTGGAGTCATTCTAGAATGGAAACTTAGGTTGGTTCCTGTTCCTCCATCTGTGACAGTTTTCGAAATTTCACAGACCATAGAACAAGGTGCAACCAAACTAGTTTCCAAATGGCAAAACATTGCAGATAAACTTCATGAAGACATTTTCCTACGTTCAGTCATAGGTGTTGGAAATAAAGCTGACGCAAAGGGTAGCAAAACTATAATAATTGAATTTGATTCCTTGTTTCTTGGGCCAGTCGAGAAACTTCTCACTTTGATGCAAGACAATTTTCCGGAGTTAGGTTTAGACCGCGGCAACTGCACTGAAATGAGCTGGATTGAGTCTGTTCTATACTTTGCCGGCATCTCAAGAAATGAGTCGGAAGCTTTGCTCGGGAGAACTCAACAATCGAAGAGCTTCTTCAAAGCAAAATCAGACTACGTGACTGAACCAATTTCGGAAGCTGGCTTGGAAGGTTTATGGCAAACACTGATTGAAGTAGAAGCTTATATGATATGGACACCTTATGGTGGAAAAATGAGTGAGATTTCGGATTCAGAAACTCCTTTCCCACACAGAATTGGCAACATATTCGAAATCCAGTATATGGTCACTTGGGATGATGGCAAGGAAACTGAGAAACATATTGGCATGATGAGAAGACTGTATGACTACATGGCACCATATGTTTCAAAGTCCCCAAGAGGTGCCTATTTGAATTATAGGGATCTTGACTTGGGGAGGAACAATGTTTGTAATACAAGCTATGGACAAGCAAGCATTTGGGGCTTAAAGTATTTCAAGAATAATTTTAGGAGACTTGTTCATGTGAAGACCTTAGTTGATCCTGGTAACTTCTTTAGGAATGAACAAAGCATCCCTGTGTTCCCATCTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

551

Amino Acids

61.72

Weight (kDa)

8.49

Isoelectric Point (pI)

50.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1432, 1460
AccB7I CCANNNNNTGG 1 cut(s) 1360
AccII CGCG 1 cut(s) 1065
AccIII TCCGGA 1 cut(s) 1045
AciI CCGC 5 cut(s) 217, 426, 615, 1063, 1065
AclI AACGTT 1 cut(s) 41
AclWI GGATC 2 cut(s) 1488, 1592
AcsI RAATTY 5 cut(s) 8, 172, 223, 834, 983
AfaI GTAC 2 cut(s) 533, 631
AfiI CCNNNNNNNGG 7 cut(s) 187, 426, 427, 593, 1052, 1283, 1360
AgsI TTSAA 9 cut(s) 19, 177, 316, 983, 1177, 1250, 1446, 1471, 1555
AhlI ACTAGT 1 cut(s) 868
AjnI CCWGG 1 cut(s) 1600
AjuI GAANNNNNNNTTGG 2 cut(s) 782, 814
AloI GAACNNNNNNTCC 2 cut(s) 1614, 1646
Alw21I GWGCWC 1 cut(s) 499
Alw26I GTCTC 2 cut(s) 30, 1564
AlwI GGATC 2 cut(s) 1488, 1592
Ama87I CYCGRG 2 cut(s) 498, 1145
Aor13HI TCCGGA 1 cut(s) 1045
AoxI GGCC 3 cut(s) 430, 445, 1004
ApeKI GCWGC 2 cut(s) 377, 656
ApoI RAATTY 5 cut(s) 8, 172, 223, 834, 983
ArsI GACNNNNNNTTYG 2 cut(s) 634, 666
Asp700I GAANNNNTTC 3 cut(s) 8, 53, 1169
AspS9I GGNCC 3 cut(s) 430, 445, 1004
AsuHPI GGTGA 1 cut(s) 244
AsuII TTCGAA 2 cut(s) 831, 1347
AvaI CYCGRG 2 cut(s) 498, 1145
BanI GGYRCC 2 cut(s) 1432, 1460
BbsI GAAGAC 3 cut(s) 915, 1420, 1592
Bbv12I GWGCWC 1 cut(s) 499
BbvI GCAGC 2 cut(s) 389, 668
BccI CCATC 5 cut(s) 97, 599, 823, 1370, 1651
BceAI ACGGC 1 cut(s) 418
BcgI CGANNNNNNTGC 4 cut(s) 688, 722, 1327, 1361
BciT130I CCWGG 1 cut(s) 1602
BcoDI GTCTC 2 cut(s) 30, 1564
BcuI ACTAGT 1 cut(s) 868
BfaI CTAG 3 cut(s) 653, 782, 869
BglII AGATCT 1 cut(s) 108
BisI GCNGC 3 cut(s) 378, 657, 1066
BlsI GCNGC 3 cut(s) 379, 658, 1067
Bme1390I CCNGG 1 cut(s) 1602
BmeT110I CYCGRG 2 cut(s) 498, 1145
BmgT120I GGNCC 3 cut(s) 430, 445, 1004
BmiI GGNNCC 4 cut(s) 431, 804, 1434, 1462
BmrFI CCNGG 1 cut(s) 1602
BmsI GCATC 4 cut(s) 664, 1020, 1125, 1638
BpiI GAAGAC 3 cut(s) 915, 1420, 1592
BplI GAGNNNNNCTC 2 cut(s) 717, 749
Bpu14I TTCGAA 2 cut(s) 831, 1347
BpuEI CTTGAG 2 cut(s) 748, 1105
Bsa29I ATCGAT 2 cut(s) 463, 471
BsaAI YACGTR 1 cut(s) 1195
BsaBI GATNNNNATC 1 cut(s) 107
BsaJI CCNNGG 2 cut(s) 720, 1063
BsaWI WCCGGW 1 cut(s) 1045
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 7 cut(s) 187, 426, 427, 593, 1052, 1283, 1360
Bse118I RCCGGY 1 cut(s) 1112
Bse1I ACTGG 2 cut(s) 1007, 1354
Bse3DI GCAATG 1 cut(s) 888
Bse8I GATNNNNATC 1 cut(s) 107
BseAI TCCGGA 1 cut(s) 1045
BseBI CCWGG 1 cut(s) 1602
BseCI ATCGAT 2 cut(s) 463, 471
BseDI CCNNGG 2 cut(s) 720, 1063
BseGI GGATG 4 cut(s) 212, 246, 1378, 1629
BseJI GATNNNNATC 1 cut(s) 107
BseLI CCNNNNNNNGG 7 cut(s) 187, 426, 427, 593, 1052, 1283, 1360
BseMI GCAATG 1 cut(s) 888
BseMII CTCAG 2 cut(s) 171, 1380
BseNI ACTGG 2 cut(s) 1007, 1354
BseRI GAGGAG 1 cut(s) 765
BseXI GCAGC 2 cut(s) 389, 668
BsgI GTGCAG 1 cut(s) 1057
Bsh1236I CGCG 1 cut(s) 1065
BshFI GGCC 3 cut(s) 432, 447, 1006
BshNI GGYRCC 2 cut(s) 1432, 1460
BshVI ATCGAT 2 cut(s) 463, 471
BsiHKAI GWGCWC 1 cut(s) 499
BsiHKCI CYCGRG 2 cut(s) 498, 1145
BsiSI CCGG 2 cut(s) 1046, 1113
BslFI GGGAC 3 cut(s) 185, 618, 1435
BslI CCNNNNNNNGG 7 cut(s) 187, 426, 427, 593, 1052, 1283, 1360
BsmAI GTCTC 2 cut(s) 30, 1564
BsmFI GGGAC 3 cut(s) 185, 618, 1435
BsnI GGCC 3 cut(s) 432, 447, 1006
BsoBI CYCGRG 2 cut(s) 498, 1145
Bsp119I TTCGAA 2 cut(s) 831, 1347
Bsp1286I GDGCHC 1 cut(s) 499
Bsp13I TCCGGA 1 cut(s) 1045
Bsp143I GATC 5 cut(s) 108, 460, 464, 1480, 1597
Bsp19I CCATGG 1 cut(s) 720
BspACI CCGC 5 cut(s) 217, 426, 615, 1063, 1065
BspANI GGCC 3 cut(s) 432, 447, 1006
BspCNI CTCAG 2 cut(s) 170, 1381
BspDI ATCGAT 2 cut(s) 463, 471
BspEI TCCGGA 1 cut(s) 1045
BspFNI CGCG 1 cut(s) 1065
BspHI TCATGA 1 cut(s) 904
BspLI GGNNCC 4 cut(s) 431, 804, 1434, 1462
BspPI GGATC 2 cut(s) 1488, 1592
BspQI GCTCTTC 1 cut(s) 1160
BspT104I TTCGAA 2 cut(s) 831, 1347
BspT107I GGYRCC 2 cut(s) 1432, 1460
BsrDI GCAATG 1 cut(s) 888
BsrFI RCCGGY 1 cut(s) 1112
BsrI ACTGG 2 cut(s) 1007, 1354
BssAI RCCGGY 1 cut(s) 1112
BssECI CCNNGG 2 cut(s) 720, 1063
BssMI GATC 5 cut(s) 108, 460, 464, 1480, 1597
BssT1I CCWWGG 1 cut(s) 720
Bst2UI CCWGG 1 cut(s) 1602
Bst4CI ACNGT 3 cut(s) 665, 826, 1419
Bst6I CTCTTC 1 cut(s) 1160
BstBAI YACGTR 1 cut(s) 1195
BstBI TTCGAA 2 cut(s) 831, 1347
BstC8I GCNNGC 3 cut(s) 1114, 1219, 1531
BstDEI CTNAG 6 cut(s) 157, 277, 678, 794, 1389, 1591
BstDSI CCRYGG 2 cut(s) 720, 1063
BstF5I GGATG 4 cut(s) 212, 246, 1378, 1629
BstFNI CGCG 1 cut(s) 1065
BstKTI GATC 5 cut(s) 111, 463, 467, 1483, 1600
BstMAI GTCTC 2 cut(s) 30, 1564
BstMBI GATC 5 cut(s) 108, 460, 464, 1480, 1597
BstMWI GCNNNNNNNGC 2 cut(s) 1071, 1539
BstNI CCWGG 1 cut(s) 1602
BstSCI CCNGG 1 cut(s) 1600
BstUI CGCG 1 cut(s) 1065
BstV1I GCAGC 2 cut(s) 389, 668
BstV2I GAAGAC 3 cut(s) 915, 1420, 1592
BstX2I RGATCY 2 cut(s) 108, 1480
BstYI RGATCY 2 cut(s) 108, 1480
Bsu15I ATCGAT 2 cut(s) 463, 471
BsuRI GGCC 3 cut(s) 432, 447, 1006
BsuTUI ATCGAT 2 cut(s) 463, 471
BtgI CCRYGG 2 cut(s) 720, 1063
BtsCI GGATG 4 cut(s) 212, 246, 1378, 1629
BtsIMutI CAGTG 3 cut(s) 670, 1074, 1241
Cac8I GCNNGC 3 cut(s) 1114, 1219, 1531
CciI TCATGA 1 cut(s) 904
Cfr10I RCCGGY 1 cut(s) 1112
Cfr13I GGNCC 3 cut(s) 430, 445, 1004
Cfr42I CCGCGG 1 cut(s) 1066
ClaI ATCGAT 2 cut(s) 463, 471
CseI GACGC 1 cut(s) 962
Csp6I GTAC 2 cut(s) 532, 630
CviQI GTAC 2 cut(s) 532, 630
DdeI CTNAG 6 cut(s) 157, 277, 678, 794, 1389, 1591
DpnI GATC 5 cut(s) 110, 462, 466, 1482, 1599
DpnII GATC 5 cut(s) 108, 460, 464, 1480, 1597
Eam1104I CTCTTC 1 cut(s) 1160
EarI CTCTTC 1 cut(s) 1160
Eco130I CCWWGG 1 cut(s) 720
Eco88I CYCGRG 2 cut(s) 498, 1145
EcoO109I RGGNCCY 1 cut(s) 445
EcoRII CCWGG 1 cut(s) 1600
EcoT14I CCWWGG 1 cut(s) 720
ErhI CCWWGG 1 cut(s) 720
FalI AAGNNNNNCTT 2 cut(s) 1157, 1189
FaqI GGGAC 3 cut(s) 185, 618, 1435
FauI CCCGC 2 cut(s) 224, 419
FauNDI CATATG 1 cut(s) 1438
Fnu4HI GCNGC 3 cut(s) 378, 657, 1066
FokI GGATG 4 cut(s) 199, 233, 1385, 1616
Fsp4HI GCNGC 3 cut(s) 378, 657, 1066
FspBI CTAG 3 cut(s) 653, 782, 869
GluI GCNGC 3 cut(s) 378, 657, 1066
HaeIII GGCC 3 cut(s) 432, 447, 1006
HapII CCGG 2 cut(s) 1046, 1113
HgaI GACGC 1 cut(s) 962
HincII GTYRAC 2 cut(s) 478, 688
HindII GTYRAC 2 cut(s) 478, 688
HindIII AAGCTT 5 cut(s) 52, 193, 766, 1137, 1257
HinfI GANTC 9 cut(s) 301, 309, 506, 703, 774, 989, 1094, 1130, 1307
HpaII CCGG 2 cut(s) 1046, 1113
HphI GGTGA 1 cut(s) 244
Hpy166II GTNNAC 2 cut(s) 478, 688
Hpy8I GTNNAC 2 cut(s) 478, 688
HpyAV CCTTC 3 cut(s) 158, 363, 1220
HpyCH4III ACNGT 3 cut(s) 665, 826, 1419
HpyCH4IV ACGT 4 cut(s) 41, 690, 922, 1194
HpyCH4V TGCA 4 cut(s) 860, 893, 1033, 1074
HpyF10VI GCNNNNNNNGC 2 cut(s) 1071, 1539
HpyF3I CTNAG 6 cut(s) 157, 277, 678, 794, 1389, 1591
HpySE526I ACGT 4 cut(s) 41, 690, 922, 1194
Kpn2I TCCGGA 1 cut(s) 1045
KroI GCCGGC 1 cut(s) 1112
KroNI GCCGGC 1 cut(s) 1114
KspI CCGCGG 1 cut(s) 1066
Kzo9I GATC 5 cut(s) 108, 460, 464, 1480, 1597
LguI GCTCTTC 1 cut(s) 1160
Lsp1109I GCAGC 2 cut(s) 389, 668
LweI GCATC 4 cut(s) 664, 1020, 1125, 1638
MaeI CTAG 3 cut(s) 653, 782, 869
MaeII ACGT 4 cut(s) 41, 690, 922, 1194
MaeIII GTNAC 4 cut(s) 820, 1195, 1363, 1604
MalI GATC 5 cut(s) 110, 462, 466, 1482, 1599
MboI GATC 5 cut(s) 108, 460, 464, 1480, 1597
MboII GAAGA 8 cut(s) 49, 136, 740, 920, 1165, 1177, 1425, 1597
MflI RGATCY 2 cut(s) 108, 1480
MhlI GDGCHC 1 cut(s) 499
MlyI GAGTC 4 cut(s) 515, 783, 1103, 1139
MmeI TCCRAC 2 cut(s) 919, 1113
MroI TCCGGA 1 cut(s) 1045
MroNI GCCGGC 1 cut(s) 1112
MroXI GAANNNNTTC 3 cut(s) 8, 53, 1169
MseI TTAA 3 cut(s) 284, 693, 1544
MslI CAYNNNNRTG 4 cut(s) 1028, 1079, 1634, 1648
MspA1I CMGCKG 3 cut(s) 380, 659, 1065
MspI CCGG 2 cut(s) 1046, 1113
MspR9I CCNGG 1 cut(s) 1602
MvaI CCWGG 1 cut(s) 1602
MvnI CGCG 1 cut(s) 1065
MwoI GCNNNNNNNGC 2 cut(s) 1071, 1539
NaeI GCCGGC 1 cut(s) 1114
NcoI CCATGG 1 cut(s) 720
NdeI CATATG 1 cut(s) 1438
NdeII GATC 5 cut(s) 108, 460, 464, 1480, 1597
NgoMIV GCCGGC 1 cut(s) 1112
NlaIV GGNNCC 4 cut(s) 431, 804, 1434, 1462
NmuCI GTSAC 3 cut(s) 820, 1195, 1363
NspV TTCGAA 2 cut(s) 831, 1347
PaeR7I CTCGAG 1 cut(s) 498
PagI TCATGA 1 cut(s) 904
PciSI GCTCTTC 1 cut(s) 1160
PdiI GCCGGC 1 cut(s) 1114
PdmI GAANNNNTTC 3 cut(s) 8, 53, 1169
PfeI GAWTC 5 cut(s) 301, 309, 703, 989, 1307
PflMI CCANNNNNTGG 1 cut(s) 1360
PkrI GCNGC 3 cut(s) 379, 658, 1067
PleI GAGTC 4 cut(s) 514, 782, 1102, 1138
PpsI GAGTC 4 cut(s) 514, 782, 1102, 1138
Ppu21I YACGTR 1 cut(s) 1195
Psp1406I AACGTT 1 cut(s) 41
Psp6I CCWGG 1 cut(s) 1600
PspGI CCWGG 1 cut(s) 1600
PspN4I GGNNCC 4 cut(s) 431, 804, 1434, 1462
PspPI GGNCC 3 cut(s) 430, 445, 1004
PspXI VCTCGAGB 1 cut(s) 498
PsuI RGATCY 2 cut(s) 108, 1480
PvuII CAGCTG 2 cut(s) 380, 659
RsaI GTAC 2 cut(s) 533, 631
RsaNI GTAC 2 cut(s) 532, 630
RseI CAYNNNNRTG 4 cut(s) 1028, 1079, 1634, 1648
SacII CCGCGG 1 cut(s) 1066
SapI GCTCTTC 1 cut(s) 1160
SaqAI TTAA 3 cut(s) 284, 693, 1544
SatI GCNGC 3 cut(s) 378, 657, 1066
Sau3AI GATC 5 cut(s) 108, 460, 464, 1480, 1597
Sau96I GGNCC 3 cut(s) 430, 445, 1004
SchI GAGTC 4 cut(s) 515, 783, 1103, 1139
ScrFI CCNGG 1 cut(s) 1602
SduI GDGCHC 1 cut(s) 499
SfaNI GCATC 4 cut(s) 664, 1020, 1125, 1638
Sfr274I CTCGAG 1 cut(s) 498
Sfr303I CCGCGG 1 cut(s) 1066
SfuI TTCGAA 2 cut(s) 831, 1347
SgrBI CCGCGG 1 cut(s) 1066
SlaI CTCGAG 1 cut(s) 498
SmiMI CAYNNNNRTG 4 cut(s) 1028, 1079, 1634, 1648
SmlI CTYRAG 3 cut(s) 498, 763, 1120
SmoI CTYRAG 3 cut(s) 498, 763, 1120
SpeI ACTAGT 1 cut(s) 868
SsiI CCGC 5 cut(s) 217, 426, 615, 1063, 1065
SspMI CTAG 3 cut(s) 653, 782, 869
StyD4I CCNGG 1 cut(s) 1600
StyI CCWWGG 1 cut(s) 720
TaaI ACNGT 3 cut(s) 665, 826, 1419
TaiI ACGT 4 cut(s) 44, 693, 925, 1197
TaqI TCGA 9 cut(s) 463, 471, 499, 708, 748, 831, 1011, 1163, 1347
TatI WGTACW 1 cut(s) 531
TauI GCSGC 1 cut(s) 1068
TfiI GAWTC 5 cut(s) 301, 309, 703, 989, 1307
Tru1I TTAA 3 cut(s) 284, 693, 1544
Tru9I TTAA 3 cut(s) 284, 693, 1544
TscAI CASTG 3 cut(s) 670, 1081, 1248
TseFI GTSAC 3 cut(s) 820, 1195, 1363
TseI GCWGC 2 cut(s) 377, 656
Tsp45I GTSAC 3 cut(s) 820, 1195, 1363
TspDTI ATGAA 9 cut(s) 17, 62, 201, 283, 551, 893, 921, 1568, 1635
TspRI CASTG 3 cut(s) 670, 1081, 1248
Van91I CCANNNNNTGG 1 cut(s) 1360
XapI RAATTY 5 cut(s) 8, 172, 223, 834, 983
XbaI TCTAGA 1 cut(s) 781
XhoI CTCGAG 1 cut(s) 498
XmnI GAANNNNTTC 3 cut(s) 8, 53, 1169
XspI CTAG 3 cut(s) 653, 782, 869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.