pycom05g25090

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000925
Physical Location & Seq
Forward (+)
26562491 .. 26563179
689 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25090.1

Sequence Viewer

Length: 597 bp
ATGTCCAACTATTGGTACGGTCTAGCAGCTGACAATGTCCTTGATGCTAAGATTGTCGACGTTAGCGGAAGAATTCTTGACAGAAAATCCATGGGAGAAGATCTCTTTTGGGCAATTCGAGGTGGCGGAGGCTCAAACTTTGGAGTCATCCTGGCCTGGAAACTTAGGTTGGTTCCTGTTCCTCCAATTGTAACAGGCTACATGGAATCGAAGACCATAGAACAAGGTGCAACCAAACTTGTTTCCAAATGGCAAGCTATAGCTGATAGGATGCCTCAACAGTACTTCCTGCGTTTGGTTATCCAAGTTGCAAATGAAACTGGTACAAATGGTGGAAAAACTATTCAAGTTACGTTCAATTTCGTGGCTTTTGAGACTGTTGAGAAAGTTCGCCCTTGGATGCAAGAAAATTTCCCTGAGTTAAATTTAGACCAGTCCACCTTCACTGAAATGAGTTGGATTGAGTCCGCTCTATACGTTGCTGACTTCCCGATAAACGAATCACAAGTTCTGCTTCAGCGGACTCAACAATCGAGGGTATACTTCAAAAACAAATCAGATTTTGTTACTGAACCTATTTCAGAAGCTTACAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

199

Amino Acids

22.48

Weight (kDa)

5.12

Isoelectric Point (pI)

41.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 12
AccBSI CCGCTC 1 cut(s) 470
AccI GTMKAC 2 cut(s) 57, 540
AciI CCGC 4 cut(s) 66, 126, 468, 520
AcsI RAATTY 3 cut(s) 72, 409, 424
AcuI CTGAAG 1 cut(s) 500
AfaI GTAC 3 cut(s) 17, 284, 325
AfiI CCNNNNNNNGG 2 cut(s) 12, 295
AgsI TTSAA 3 cut(s) 347, 358, 547
AjnI CCWGG 2 cut(s) 150, 155
AjuI GAANNNNNNNTTGG 2 cut(s) 152, 184
AluBI AGCT 4 cut(s) 29, 257, 263, 587
AluI AGCT 4 cut(s) 29, 257, 263, 587
Alw26I GTCTC 1 cut(s) 368
AoxI GGCC 1 cut(s) 153
ApeKI GCWGC 1 cut(s) 26
ApoI RAATTY 3 cut(s) 72, 409, 424
BbsI GAAGAC 1 cut(s) 218
BbvI GCAGC 1 cut(s) 38
BciT130I CCWGG 2 cut(s) 152, 157
BcoDI GTCTC 1 cut(s) 368
BfaI CTAG 1 cut(s) 23
BfmI CTRYAG 1 cut(s) 258
BglII AGATCT 1 cut(s) 100
BisI GCNGC 1 cut(s) 27
BlsI GCNGC 1 cut(s) 28
BmcAI AGTACT 1 cut(s) 284
Bme1390I CCNGG 2 cut(s) 152, 157
BmiI GGNNCC 1 cut(s) 174
BmrFI CCNGG 2 cut(s) 152, 157
BmsI GCATC 3 cut(s) 34, 261, 390
BpiI GAAGAC 1 cut(s) 218
BplI GAGNNNNNCTC 2 cut(s) 87, 119
BsaJI CCNNGG 2 cut(s) 90, 395
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 295
Bse1I ACTGG 2 cut(s) 325, 433
BseBI CCWGG 2 cut(s) 152, 157
BseDI CCNNGG 2 cut(s) 90, 395
BseGI GGATG 3 cut(s) 147, 276, 405
BseLI CCNNNNNNNGG 2 cut(s) 12, 295
BseMII CTCAG 1 cut(s) 408
BseNI ACTGG 2 cut(s) 325, 433
BseXI GCAGC 1 cut(s) 38
BshFI GGCC 1 cut(s) 155
BslI CCNNNNNNNGG 2 cut(s) 12, 295
BsmAI GTCTC 1 cut(s) 368
BsnI GGCC 1 cut(s) 155
Bsp143I GATC 1 cut(s) 100
Bsp19I CCATGG 1 cut(s) 90
BspACI CCGC 4 cut(s) 66, 126, 468, 520
BspANI GGCC 1 cut(s) 155
BspCNI CTCAG 1 cut(s) 409
BspLI GGNNCC 1 cut(s) 174
BsrBI CCGCTC 1 cut(s) 470
BsrI ACTGG 2 cut(s) 325, 433
BssECI CCNNGG 2 cut(s) 90, 395
BssMI GATC 1 cut(s) 100
BssNAI GTATAC 1 cut(s) 541
BssT1I CCWWGG 2 cut(s) 90, 395
Bst1107I GTATAC 1 cut(s) 541
Bst2UI CCWGG 2 cut(s) 152, 157
Bst4CI ACNGT 4 cut(s) 20, 282, 379, 593
BstC8I GCNNGC 1 cut(s) 255
BstDEI CTNAG 3 cut(s) 48, 164, 417
BstDSI CCRYGG 1 cut(s) 90
BstF5I GGATG 3 cut(s) 147, 276, 405
BstKTI GATC 1 cut(s) 103
BstMAI GTCTC 1 cut(s) 368
BstMBI GATC 1 cut(s) 100
BstNI CCWGG 2 cut(s) 152, 157
BstSCI CCNGG 2 cut(s) 150, 155
BstSFI CTRYAG 1 cut(s) 258
BstV1I GCAGC 1 cut(s) 38
BstV2I GAAGAC 1 cut(s) 218
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BstZ17I GTATAC 1 cut(s) 541
BsuRI GGCC 1 cut(s) 155
BtgI CCRYGG 1 cut(s) 90
BtsCI GGATG 3 cut(s) 147, 276, 405
BtsIMutI CAGTG 1 cut(s) 444
Cac8I GCNNGC 1 cut(s) 255
Csp6I GTAC 3 cut(s) 16, 283, 324
CviAII CATG 2 cut(s) 91, 202
CviJI RGCY 8 cut(s) 29, 132, 155, 198, 257, 263, 368, 587
CviKI_1 RGCY 8 cut(s) 29, 132, 155, 198, 257, 263, 368, 587
CviQI GTAC 3 cut(s) 16, 283, 324
DdeI CTNAG 3 cut(s) 48, 164, 417
DpnI GATC 1 cut(s) 102
DpnII GATC 1 cut(s) 100
EciI GGCGGA 1 cut(s) 141
Eco130I CCWWGG 2 cut(s) 90, 395
Eco57I CTGAAG 1 cut(s) 500
EcoRI GAATTC 1 cut(s) 72
EcoRII CCWGG 2 cut(s) 150, 155
EcoT14I CCWWGG 2 cut(s) 90, 395
ErhI CCWWGG 2 cut(s) 90, 395
FaeI CATG 2 cut(s) 94, 205
FaiI YATR 6 cut(s) 92, 203, 218, 260, 475, 541
FalI AAGNNNNNCTT 2 cut(s) 498, 530
FatI CATG 2 cut(s) 90, 201
FblI GTMKAC 2 cut(s) 57, 540
Fnu4HI GCNGC 1 cut(s) 27
FokI GGATG 3 cut(s) 134, 283, 412
Fsp4HI GCNGC 1 cut(s) 27
FspBI CTAG 1 cut(s) 23
GluI GCNGC 1 cut(s) 27
HaeIII GGCC 1 cut(s) 155
Hin1II CATG 2 cut(s) 94, 205
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HindIII AAGCTT 1 cut(s) 585
HinfI GANTC 5 cut(s) 144, 206, 464, 500, 523
Hpy166II GTNNAC 3 cut(s) 58, 438, 541
Hpy188I TCNGA 2 cut(s) 559, 583
Hpy188III TCNNGA 2 cut(s) 77, 490
Hpy8I GTNNAC 3 cut(s) 58, 438, 541
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 1 cut(s) 451
HpyCH4III ACNGT 4 cut(s) 20, 282, 379, 593
HpyCH4IV ACGT 3 cut(s) 60, 353, 477
HpyCH4V TGCA 3 cut(s) 230, 311, 403
HpyF3I CTNAG 3 cut(s) 48, 164, 417
HpySE526I ACGT 3 cut(s) 60, 353, 477
Hsp92II CATG 2 cut(s) 94, 205
Kzo9I GATC 1 cut(s) 100
Lsp1109I GCAGC 1 cut(s) 38
LweI GCATC 3 cut(s) 34, 261, 390
MaeI CTAG 1 cut(s) 23
MaeII ACGT 3 cut(s) 60, 353, 477
MaeIII GTNAC 3 cut(s) 190, 349, 565
MalI GATC 1 cut(s) 102
MbiI CCGCTC 1 cut(s) 470
MboI GATC 1 cut(s) 100
MboII GAAGA 3 cut(s) 81, 110, 223
MfeI CAATTG 1 cut(s) 186
MflI RGATCY 1 cut(s) 100
MluCI AATT 6 cut(s) 72, 114, 186, 358, 409, 424
MlyI GAGTC 3 cut(s) 153, 473, 517
MmeI TCCRAC 2 cut(s) 30, 437
MnlI CCTC 5 cut(s) 113, 122, 192, 285, 528
MseI TTAA 1 cut(s) 422
MslI CAYNNNNRTG 1 cut(s) 449
MspA1I CMGCKG 2 cut(s) 29, 520
MspR9I CCNGG 2 cut(s) 152, 157
MunI CAATTG 1 cut(s) 186
MvaI CCWGG 2 cut(s) 152, 157
NcoI CCATGG 1 cut(s) 90
NdeII GATC 1 cut(s) 100
NlaIII CATG 2 cut(s) 94, 205
NlaIV GGNNCC 1 cut(s) 174
PfeI GAWTC 2 cut(s) 206, 500
PflFI GACNNNGTC 1 cut(s) 35
PflMI CCANNNNNTGG 1 cut(s) 12
PkrI GCNGC 1 cut(s) 28
PleI GAGTC 3 cut(s) 152, 472, 517
PpsI GAGTC 3 cut(s) 152, 472, 517
Psp6I CCWGG 2 cut(s) 150, 155
PspGI CCWGG 2 cut(s) 150, 155
PspN4I GGNNCC 1 cut(s) 174
PsuI RGATCY 1 cut(s) 100
PsyI GACNNNGTC 1 cut(s) 35
PvuII CAGCTG 1 cut(s) 29
RsaI GTAC 3 cut(s) 17, 284, 325
RsaNI GTAC 3 cut(s) 16, 283, 324
RseI CAYNNNNRTG 1 cut(s) 449
SalI GTCGAC 1 cut(s) 56
SaqAI TTAA 1 cut(s) 422
SatI GCNGC 1 cut(s) 27
Sau3AI GATC 1 cut(s) 100
ScaI AGTACT 1 cut(s) 284
SchI GAGTC 3 cut(s) 153, 473, 517
ScrFI CCNGG 2 cut(s) 152, 157
SfaNI GCATC 3 cut(s) 34, 261, 390
SfcI CTRYAG 1 cut(s) 258
SmiMI CAYNNNNRTG 1 cut(s) 449
Sse9I AATT 6 cut(s) 72, 114, 186, 358, 409, 424
SsiI CCGC 4 cut(s) 66, 126, 468, 520
SspMI CTAG 1 cut(s) 23
StyD4I CCNGG 2 cut(s) 150, 155
StyI CCWWGG 2 cut(s) 90, 395
TaaI ACNGT 4 cut(s) 20, 282, 379, 593
TaiI ACGT 3 cut(s) 63, 356, 480
TaqI TCGA 4 cut(s) 57, 118, 209, 533
TasI AATT 6 cut(s) 72, 114, 186, 358, 409, 424
TatI WGTACW 1 cut(s) 282
TfiI GAWTC 2 cut(s) 206, 500
Tru1I TTAA 1 cut(s) 422
Tru9I TTAA 1 cut(s) 422
TscAI CASTG 1 cut(s) 451
TseI GCWGC 1 cut(s) 26
TspDTI ATGAA 1 cut(s) 330
TspRI CASTG 1 cut(s) 451
Tth111I GACNNNGTC 1 cut(s) 35
Van91I CCANNNNNTGG 1 cut(s) 12
XapI RAATTY 3 cut(s) 72, 409, 424
XmiI GTMKAC 2 cut(s) 57, 540
XspI CTAG 1 cut(s) 23
ZrmI AGTACT 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.