Rw5G011430

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000925
Physical Location & Seq
Forward (+)
13010127 .. 13011707
1581 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G011430.1

Sequence Viewer

Length: 1581 bp
ATGGAGACCTTTCATGTAAAACTGTTTTCTCTACTTCTCATCCTTCTCAACTCAATTTGGTCCACAACTTCAACATCAAATTCTGAAAGCTTTGTTCAATGCTTTTCCTCCCACCTACAGAACTCTAACTCCAGTAACGAAATCATACTCACCAGAACCAGTTCTGCTTATCCCTCAGTCTTAGATTTTTCCATACACAACTTCAGATTCTTGAACAATTCCACACCAAAACCAGAGGCCATCATTACTCCTTTTGATGAATCTCAGGTTCAAGCAGCAGTAATTTGCTCCGAGAAAAGTGGCATACAAATAAGAATCCGAAGCGGGGGGCATGATTACGAGGGCCTATCTTATGTATCCAGAGCTCCTTTCATCATCATTGATCTTTTCAATCTCCGTTCCATCGATGTTGATATAAAGAATGAGAGTGCTTGGGTCCAATCAGGCGCTACTCTCGGAGAGTTGTATTACAACATTGCACAGAAGAGTATAGTCTATGGCTTTCCAGCAGGGACTTGTCCCACCGTCGGTGTTGGGGGACACATCAGTGGGGGTGGATATGGAACCATATTCCGAAAATATGGTCTAGCATCCGACAATGTCATTGATGCTAGAATTGTAGATGTTAACGGCAGAATTCTAGACAGAAAGTCCATGGGAGAAGAGCTCTTTTGGGCTATCAGAGGAGGAGGAGGATCAAGCTTTGGTGTCATTCTAGCATGGAAACTTAGACTGGTTCCTGTTCCCCCAACTGTAACAGTTTGCGTCGTTACAAAGACAAAAGAACAAGGTGCAACCAAACTTCTTTTGAAATGGCAAAACATTGCAGACAAGCTTCCTGAAGACCTTTTCCTACATTCAGTTGTAGGAAGTAGTGACAACACTAATACAGTTTTATTCGCTTCCCTGTTTCTTGGGCGGGTCGACAAAGCTGTCCAGGTTATACAGGAAAACTTCCCAGAGTTGAGTTTAGAAAGCAGCCATTGCAGTGAGATGAGTTGGATTCAGTCTGTTTTGAAATTTTCTGGTTACTCAATGCACGAACCCTTAGAGATTTTGCTGAACCGCCGGCAACTCAGCTACTTCTACTTCTTCAAAGCAAAATCAGACTTTGTGACTGAACCCATCTCAGAAGCTGGCTTGGAAGGCTTATGGCACATACTGCTTGAAGCAAACACATCCTATATGATATTGACACCATATGGAGGAAAAATGAGTGAGATTTCCGATTCAGAAACTGCTTTCCCACATCGAAAAGGTAACAAATATGAAATCATGTATATGGTTACTTGGGGTGATGGCAAGGACACGAAGAAGTATGTTGGATTTATGAGAAGGCTGTATGCATACATGGCCCCGTATGTTTCGAAGTCTCCTAGAGCTGCCTATTTGAATTATAGGGATCTTGATTTGGGGAGGAACAGTTATGGCAATACAAGCTATGCACAAGCAAGCATTTGGGGTTTGAAGTACTTCAAGAACAACTTTAGGAGACTTGTTCATGTTAAGACCTTGGTTGATCCTGGCAACTTCTTTAGGAATGAGCAAAGCATCCCCGTGTTTTCGTCAGGAAAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

526

Amino Acids

58.93

Weight (kDa)

8.68

Isoelectric Point (pI)

43.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 78 - 214 8.9e-27 FAD binding domain
BBE PF08031 462 - 519 2.2e-18 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 932
AccI GTMKAC 1 cut(s) 924
AciI CCGC 3 cut(s) 324, 919, 1066
AclWI GGATC 3 cut(s) 703, 1410, 1514
AcsI RAATTY 3 cut(s) 79, 636, 1019
AcuI CTGAAG 2 cut(s) 187, 861
AfaI GTAC 1 cut(s) 1472
AfiI CCNNNNNNNGG 3 cut(s) 325, 527, 1205
AhdI GACNNNNNGTC 1 cut(s) 649
AjnI CCWGG 2 cut(s) 936, 1522
AleI CACNNNNGTG 1 cut(s) 546
AloI GAACNNNNNNTCC 2 cut(s) 113, 145
Alw21I GWGCWC 2 cut(s) 367, 669
Alw26I GTCTC 2 cut(s) 1377, 1486
AlwI GGATC 3 cut(s) 703, 1410, 1514
AlwNI CAGNNNCTG 2 cut(s) 1136, 1238
AoxI GGCC 3 cut(s) 237, 343, 1353
ApeKI GCWGC 3 cut(s) 275, 978, 1382
ApoI RAATTY 3 cut(s) 79, 636, 1019
ArsI GACNNNNNNTTYG 2 cut(s) 568, 600
Asp700I GAANNNNTTC 2 cut(s) 160, 1472
AspLEI GCGC 1 cut(s) 449
AspS9I GGNCC 4 cut(s) 60, 343, 436, 1354
AsuHPI GGTGA 2 cut(s) 142, 1307
AsuII TTCGAA 1 cut(s) 1367
AvaII GGWCC 2 cut(s) 60, 436
BanII GRGCYC 2 cut(s) 367, 669
BbsI GAAGAC 1 cut(s) 849
Bbv12I GWGCWC 2 cut(s) 367, 669
BbvI GCAGC 3 cut(s) 287, 990, 1369
BccI CCATC 4 cut(s) 248, 410, 1133, 1292
BceAI ACGGC 1 cut(s) 646
BciT130I CCWGG 2 cut(s) 938, 1524
BciVI GTATCC 1 cut(s) 367
BcoDI GTCTC 2 cut(s) 1377, 1486
BfaI CTAG 5 cut(s) 587, 612, 641, 716, 1377
BfmI CTRYAG 1 cut(s) 116
BfoI RGCGCY 1 cut(s) 450
BfuI GTATCC 1 cut(s) 367
BisI GCNGC 3 cut(s) 276, 979, 1383
BlsI GCNGC 3 cut(s) 277, 980, 1384
BmcAI AGTACT 1 cut(s) 1472
Bme1390I CCNGG 2 cut(s) 938, 1524
Bme18I GGWCC 2 cut(s) 60, 436
BmeRI GACNNNNNGTC 1 cut(s) 649
BmgT120I GGNCC 4 cut(s) 60, 343, 436, 1354
BmiI GGNNCC 4 cut(s) 437, 565, 738, 1356
BmrFI CCNGG 2 cut(s) 938, 1524
BmsI GCATC 3 cut(s) 598, 599, 1560
BpiI GAAGAC 1 cut(s) 849
BplI GAGNNNNNCTC 2 cut(s) 651, 683
BpmI CTGGAG 1 cut(s) 115
Bpu14I TTCGAA 1 cut(s) 1367
Bsa29I ATCGAT 1 cut(s) 405
BsaJI CCNNGG 2 cut(s) 654, 1512
BsaXI ACNNNNNCTCC 4 cut(s) 113, 143, 1408, 1438
Bsc4I CCNNNNNNNGG 3 cut(s) 325, 527, 1205
Bse118I RCCGGY 1 cut(s) 1068
Bse1I ACTGG 3 cut(s) 132, 159, 738
Bse3DI GCAATG 3 cut(s) 474, 822, 982
BseBI CCWGG 2 cut(s) 938, 1524
BseCI ATCGAT 1 cut(s) 405
BseDI CCNNGG 2 cut(s) 654, 1512
BseGI GGATG 4 cut(s) 39, 590, 1178, 1551
BseLI CCNNNNNNNGG 3 cut(s) 325, 527, 1205
BseMI GCAATG 3 cut(s) 474, 822, 982
BseMII CTCAG 4 cut(s) 189, 278, 1090, 1143
BseNI ACTGG 3 cut(s) 132, 159, 738
BseRI GAGGAG 3 cut(s) 699, 702, 705
BseXI GCAGC 3 cut(s) 287, 990, 1369
BshFI GGCC 3 cut(s) 239, 345, 1355
BshVI ATCGAT 1 cut(s) 405
BsiHKAI GWGCWC 2 cut(s) 367, 669
BsiSI CCGG 1 cut(s) 1069
BslFI GGGAC 3 cut(s) 504, 526, 552
BslI CCNNNNNNNGG 3 cut(s) 325, 527, 1205
BsmAI GTCTC 2 cut(s) 1377, 1486
BsmFI GGGAC 3 cut(s) 504, 526, 552
BsnI GGCC 3 cut(s) 239, 345, 1355
Bsp119I TTCGAA 1 cut(s) 1367
Bsp1286I GDGCHC 2 cut(s) 367, 669
Bsp143I GATC 4 cut(s) 382, 695, 1402, 1519
Bsp19I CCATGG 1 cut(s) 654
BspACI CCGC 3 cut(s) 324, 919, 1066
BspANI GGCC 3 cut(s) 239, 345, 1355
BspCNI CTCAG 4 cut(s) 188, 277, 1089, 1142
BspDI ATCGAT 1 cut(s) 405
BspLI GGNNCC 4 cut(s) 437, 565, 738, 1356
BspPI GGATC 3 cut(s) 703, 1410, 1514
BspQI GCTCTTC 1 cut(s) 657
BspT104I TTCGAA 1 cut(s) 1367
BsrDI GCAATG 3 cut(s) 474, 822, 982
BsrFI RCCGGY 1 cut(s) 1068
BsrI ACTGG 3 cut(s) 132, 159, 738
BssAI RCCGGY 1 cut(s) 1068
BssECI CCNNGG 2 cut(s) 654, 1512
BssMI GATC 4 cut(s) 382, 695, 1402, 1519
BssT1I CCWWGG 2 cut(s) 654, 1512
Bst2UI CCWGG 2 cut(s) 938, 1524
Bst4CI ACNGT 6 cut(s) 24, 526, 754, 760, 892, 1424
Bst6I CTCTTC 2 cut(s) 479, 657
BstAPI GCANNNNNTGC 2 cut(s) 984, 1162
BstBI TTCGAA 1 cut(s) 1367
BstC8I GCNNGC 3 cut(s) 1070, 1138, 1453
BstDEI CTNAG 7 cut(s) 175, 181, 264, 728, 1048, 1076, 1129
BstDSI CCRYGG 1 cut(s) 654
BstF5I GGATG 4 cut(s) 39, 590, 1178, 1551
BstH2I RGCGCY 1 cut(s) 450
BstHHI GCGC 1 cut(s) 449
BstKTI GATC 4 cut(s) 385, 698, 1405, 1522
BstMAI GTCTC 2 cut(s) 1377, 1486
BstMBI GATC 4 cut(s) 382, 695, 1402, 1519
BstMWI GCNNNNNNNGC 5 cut(s) 984, 1146, 1162, 1352, 1437
BstNI CCWGG 2 cut(s) 938, 1524
BstSCI CCNGG 2 cut(s) 936, 1522
BstSFI CTRYAG 1 cut(s) 116
BstV1I GCAGC 3 cut(s) 287, 990, 1369
BstV2I GAAGAC 1 cut(s) 849
BstX2I RGATCY 1 cut(s) 1402
BstYI RGATCY 1 cut(s) 1402
Bsu15I ATCGAT 1 cut(s) 405
BsuI GTATCC 1 cut(s) 367
BsuRI GGCC 3 cut(s) 239, 345, 1355
BsuTUI ATCGAT 1 cut(s) 405
BtgI CCRYGG 1 cut(s) 654
BtsCI GGATG 4 cut(s) 39, 590, 1178, 1551
BtsI GCAGTG 1 cut(s) 994
BtsIMutI CAGTG 2 cut(s) 553, 994
Cac8I GCNNGC 3 cut(s) 1070, 1138, 1453
CaiI CAGNNNCTG 2 cut(s) 1136, 1238
CfoI GCGC 1 cut(s) 449
Cfr10I RCCGGY 1 cut(s) 1068
Cfr13I GGNCC 4 cut(s) 60, 343, 436, 1354
ClaI ATCGAT 1 cut(s) 405
CseI GACGC 1 cut(s) 754
Csp6I GTAC 1 cut(s) 1471
CviAII CATG 7 cut(s) 14, 332, 655, 720, 1276, 1351, 1502
CviQI GTAC 1 cut(s) 1471
DdeI CTNAG 7 cut(s) 175, 181, 264, 728, 1048, 1076, 1129
DpnI GATC 4 cut(s) 384, 697, 1404, 1521
DpnII GATC 4 cut(s) 382, 695, 1402, 1519
DrdI GACNNNNNNGTC 1 cut(s) 932
DriI GACNNNNNGTC 1 cut(s) 649
DseDI GACNNNNNNGTC 1 cut(s) 932
Eam1104I CTCTTC 2 cut(s) 479, 657
Eam1105I GACNNNNNGTC 1 cut(s) 649
EarI CTCTTC 2 cut(s) 479, 657
Ecl136II GAGCTC 2 cut(s) 365, 667
Eco130I CCWWGG 2 cut(s) 654, 1512
Eco24I GRGCYC 2 cut(s) 367, 669
Eco47I GGWCC 2 cut(s) 60, 436
Eco53kI GAGCTC 2 cut(s) 365, 667
Eco57I CTGAAG 2 cut(s) 187, 861
EcoICRI GAGCTC 2 cut(s) 365, 667
EcoO109I RGGNCCY 1 cut(s) 343
EcoRI GAATTC 1 cut(s) 636
EcoRII CCWGG 2 cut(s) 936, 1522
EcoT14I CCWWGG 2 cut(s) 654, 1512
EcoT22I ATGCAT 1 cut(s) 1348
EcoT38I GRGCYC 2 cut(s) 367, 669
ErhI CCWWGG 2 cut(s) 654, 1512
FaeI CATG 7 cut(s) 17, 335, 658, 723, 1279, 1354, 1505
FalI AAGNNNNNCTT 2 cut(s) 1469, 1501
FaqI GGGAC 3 cut(s) 504, 526, 552
FatI CATG 7 cut(s) 13, 331, 654, 719, 1275, 1350, 1501
FauI CCCGC 2 cut(s) 317, 912
FauNDI CATATG 1 cut(s) 1201
FblI GTMKAC 1 cut(s) 924
Fnu4HI GCNGC 3 cut(s) 276, 979, 1383
FokI GGATG 4 cut(s) 26, 577, 1165, 1538
FriOI GRGCYC 2 cut(s) 367, 669
Fsp4HI GCNGC 3 cut(s) 276, 979, 1383
FspBI CTAG 5 cut(s) 587, 612, 641, 716, 1377
GlaI GCGC 1 cut(s) 448
GluI GCNGC 3 cut(s) 276, 979, 1383
GsuI CTGGAG 1 cut(s) 115
HaeII RGCGCY 1 cut(s) 450
HaeIII GGCC 3 cut(s) 239, 345, 1355
HapII CCGG 1 cut(s) 1069
HgaI GACGC 1 cut(s) 754
HhaI GCGC 1 cut(s) 449
Hin1II CATG 7 cut(s) 17, 335, 658, 723, 1279, 1354, 1505
Hin6I GCGC 1 cut(s) 447
HinP1I GCGC 1 cut(s) 447
HincII GTYRAC 2 cut(s) 628, 925
HindII GTYRAC 2 cut(s) 628, 925
HindIII AAGCTT 3 cut(s) 88, 700, 833
HinfI GANTC 5 cut(s) 207, 260, 315, 1003, 1229
HpaI GTTAAC 1 cut(s) 628
HpaII CCGG 1 cut(s) 1069
HphI GGTGA 2 cut(s) 142, 1307
Hpy166II GTNNAC 3 cut(s) 63, 628, 925
Hpy188III TCNNGA 7 cut(s) 211, 360, 641, 839, 1406, 1477, 1569
Hpy8I GTNNAC 3 cut(s) 63, 628, 925
Hpy99I CGWCG 2 cut(s) 530, 770
HpyAV CCTTC 3 cut(s) 53, 1139, 1329
HpyCH4III ACNGT 6 cut(s) 24, 526, 754, 760, 892, 1424
HpyCH4V TGCA 7 cut(s) 479, 794, 827, 987, 1039, 1346, 1445
HpyF10VI GCNNNNNNNGC 5 cut(s) 984, 1146, 1162, 1352, 1437
HpyF3I CTNAG 7 cut(s) 175, 181, 264, 728, 1048, 1076, 1129
Hsp92II CATG 7 cut(s) 17, 335, 658, 723, 1279, 1354, 1505
HspAI GCGC 1 cut(s) 447
KroI GCCGGC 1 cut(s) 1068
KroNI GCCGGC 1 cut(s) 1070
KspAI GTTAAC 1 cut(s) 628
Kzo9I GATC 4 cut(s) 382, 695, 1402, 1519
LguI GCTCTTC 1 cut(s) 657
LmnI GCTCC 2 cut(s) 293, 370
Lsp1109I GCAGC 3 cut(s) 287, 990, 1369
LweI GCATC 3 cut(s) 598, 599, 1560
MaeI CTAG 5 cut(s) 587, 612, 641, 716, 1377
MaeIII GTNAC 8 cut(s) 134, 754, 769, 875, 1028, 1114, 1259, 1285
MalI GATC 4 cut(s) 384, 697, 1404, 1521
MboI GATC 4 cut(s) 382, 695, 1402, 1519
MboII GAAGA 5 cut(s) 496, 674, 854, 1084, 1324
MflI RGATCY 1 cut(s) 1402
MhlI GDGCHC 2 cut(s) 367, 669
MluCI AATT 8 cut(s) 54, 79, 217, 282, 615, 636, 1019, 1393
MmeI TCCRAC 3 cut(s) 618, 980, 1303
Mph1103I ATGCAT 1 cut(s) 1348
MroNI GCCGGC 1 cut(s) 1068
MroXI GAANNNNTTC 2 cut(s) 160, 1472
MseI TTAA 2 cut(s) 627, 1506
MslI CAYNNNNRTG 4 cut(s) 546, 987, 1280, 1556
MspI CCGG 1 cut(s) 1069
MspR9I CCNGG 2 cut(s) 938, 1524
MvaI CCWGG 2 cut(s) 938, 1524
MwoI GCNNNNNNNGC 5 cut(s) 984, 1146, 1162, 1352, 1437
NaeI GCCGGC 1 cut(s) 1070
NcoI CCATGG 1 cut(s) 654
NdeI CATATG 1 cut(s) 1201
NdeII GATC 4 cut(s) 382, 695, 1402, 1519
NgoMIV GCCGGC 1 cut(s) 1068
NlaIII CATG 7 cut(s) 17, 335, 658, 723, 1279, 1354, 1505
NlaIV GGNNCC 4 cut(s) 437, 565, 738, 1356
NmuCI GTSAC 2 cut(s) 875, 1114
NsiI ATGCAT 1 cut(s) 1348
NspV TTCGAA 1 cut(s) 1367
OliI CACNNNNGTG 1 cut(s) 546
PciSI GCTCTTC 1 cut(s) 657
PdiI GCCGGC 1 cut(s) 1070
PdmI GAANNNNTTC 2 cut(s) 160, 1472
PfeI GAWTC 5 cut(s) 207, 260, 315, 1003, 1229
PflFI GACNNNGTC 1 cut(s) 599
PkrI GCNGC 3 cut(s) 277, 980, 1384
Psp124BI GAGCTC 2 cut(s) 367, 669
Psp6I CCWGG 2 cut(s) 936, 1522
PspGI CCWGG 2 cut(s) 936, 1522
PspN4I GGNNCC 4 cut(s) 437, 565, 738, 1356
PspPI GGNCC 4 cut(s) 60, 343, 436, 1354
PstNI CAGNNNCTG 2 cut(s) 1136, 1238
PsuI RGATCY 1 cut(s) 1402
PsyI GACNNNGTC 1 cut(s) 599
RsaI GTAC 1 cut(s) 1472
RsaNI GTAC 1 cut(s) 1471
RseI CAYNNNNRTG 4 cut(s) 546, 987, 1280, 1556
SacI GAGCTC 2 cut(s) 367, 669
SalI GTCGAC 1 cut(s) 923
SapI GCTCTTC 1 cut(s) 657
SaqAI TTAA 2 cut(s) 627, 1506
SatI GCNGC 3 cut(s) 276, 979, 1383
Sau3AI GATC 4 cut(s) 382, 695, 1402, 1519
Sau96I GGNCC 4 cut(s) 60, 343, 436, 1354
ScaI AGTACT 1 cut(s) 1472
ScrFI CCNGG 2 cut(s) 938, 1524
SduI GDGCHC 2 cut(s) 367, 669
SfaNI GCATC 3 cut(s) 598, 599, 1560
SfcI CTRYAG 1 cut(s) 116
SfuI TTCGAA 1 cut(s) 1367
SinI GGWCC 2 cut(s) 60, 436
SmiMI CAYNNNNRTG 4 cut(s) 546, 987, 1280, 1556
Sse9I AATT 8 cut(s) 54, 79, 217, 282, 615, 636, 1019, 1393
SsiI CCGC 3 cut(s) 324, 919, 1066
SspMI CTAG 5 cut(s) 587, 612, 641, 716, 1377
SstI GAGCTC 2 cut(s) 367, 669
StyD4I CCNGG 2 cut(s) 936, 1522
StyI CCWWGG 2 cut(s) 654, 1512
TaaI ACNGT 6 cut(s) 24, 526, 754, 760, 892, 1424
TaqI TCGA 4 cut(s) 405, 924, 1252, 1367
TasI AATT 8 cut(s) 54, 79, 217, 282, 615, 636, 1019, 1393
TatI WGTACW 1 cut(s) 1470
TfiI GAWTC 5 cut(s) 207, 260, 315, 1003, 1229
Tru1I TTAA 2 cut(s) 627, 1506
Tru9I TTAA 2 cut(s) 627, 1506
TscAI CASTG 2 cut(s) 553, 994
TseFI GTSAC 2 cut(s) 875, 1114
TseI GCWGC 3 cut(s) 275, 978, 1382
Tsp45I GTSAC 2 cut(s) 875, 1114
TspDTI ATGAA 4 cut(s) 273, 361, 1284, 1490
TspGWI ACGGA 1 cut(s) 386
TspRI CASTG 2 cut(s) 553, 994
Tth111I GACNNNGTC 1 cut(s) 599
VpaK11BI GGWCC 2 cut(s) 60, 436
XapI RAATTY 3 cut(s) 79, 636, 1019
XbaI TCTAGA 1 cut(s) 640
XmiI GTMKAC 1 cut(s) 924
XmnI GAANNNNTTC 2 cut(s) 160, 1472
XspI CTAG 5 cut(s) 587, 612, 641, 716, 1377
ZrmI AGTACT 1 cut(s) 1472
Zsp2I ATGCAT 1 cut(s) 1348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.