AT5G66055

ankyrin repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001128
Physical Location & Seq
Reverse (-)
26417156 .. 26419363
2208 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66055.2

Sequence Viewer

Length: 1308 bp
ATGCAGTCACTCTCCACCCCACACACCATCTCTCTTCTTCTCCCCAGAACCTCTCCGTCTCGTCTTTCTCCCTCTCTTCACTCCCTTGCTTTCCCCACTCGATTACGGTCTCTTTCCTATTCTTCTCAAACGTCGATCCTCCCCGATGCCGGCGATGATTTCATTGTCGGTGACTGTCTCGTCTACGAGGACGGCGTCTTCGAAGACCCTTACCTTGATAAGGAGGTCACTCAGGTTGCGAAGCAGGAGCGCAAGAAGAATCGGCGTGGCGGGGCTAAGAGATTAGATGAATCCGAGATTGAGCCCGAGAACCTCGTGCCAGAGGAATGGAGGGATATTCAGGCGGAGGTGAATCTGACGAAGAAGGACAAGCGCAAAATAGCGCAGGAGATGGAGTTCGGGGTTCGGGTGGAGAAGAAGAGGCAAGGGCTAATTCCGCTGAGGAAAGTTGACTTGAATGACTTTCTCACGTACAAGGAAGCCAAGTTGGCTCAATTGAGGCCTGTCATTCTCGATAAACCGGGAAATTTCTCCGACGACAGTGGAGCGTCAAGCGATGGAGAGACCGCTGTATCATCTCCCAGCGAGCGAGTGGCTCCTAAGAACCCTAGATGGGCAGTTTACGGAAAGGGATTCGACCACGTTGCCAAGTTCTTCAATAGCGACAAGTACGATCCCAGCGACAAGAAATCCGACGGCCCTCGAAAGCTGCTTTCAAAAGAAGAGAAGTTTATGCTCAATAGCCGGAATCCTGACCTAGCCGTTGCCACATCAAAAAAATGGCTTCCTCTTCACACACTGGCAGCATGTGGAGAGTTTTATCTGGTTGATTCCTTGCTAAAGCACAATCTTGATATCAATGCAACCGATGTGGGCGGCTTGACAGTACTTCACCGAGCAATCATTGGTAAGAAGCAGGCTATTACTAACTACCTGCTGAGGGAATCGGCAAATCCATTTGTTCTTGATGACGAAGGTGCGACCTTGATGCACTATGCTGTGCAAACAGCATCAGCTCCCACAATAAAACTTCTCCTACTGTATAACGCTGATATAAACGCTCAGGACAGGGACGGGTGGACTCCACTGCACGTTGCAGTACAGGCCAGAAGAAGCGACATTGTAAAGCTTCTTTTGATAAAAGGGGCGGACATAGAAGTGAAGAACAAGGATGGGTTAACTCCGCTTGGGCTTTGCCTCTACCTTGGAAGAGAGATAAGGACGTATGAGGTGATGAAGCTGTTGAAAGAGTTTCCACTTAGCAGACACAAGAAGAGATTGGTAACAACAGATGAAGATATTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 RRM_1

Protein Analysis

435

Amino Acids

48.88

Weight (kDa)

8.17

Isoelectric Point (pI)

45.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 252 - 319 5.2e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 264 - 356 1.3e-11 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 179
Acc36I ACCTGC 1 cut(s) 942
AccI GTMKAC 1 cut(s) 183
AciI CCGC 7 cut(s) 270, 344, 437, 567, 876, 1148, 1184
AclWI GGATC 2 cut(s) 130, 668
AcsI RAATTY 1 cut(s) 526
AcyI GRCGYC 1 cut(s) 195
AfaI GTAC 4 cut(s) 473, 671, 888, 1101
AfiI CCNNNNNNNGG 4 cut(s) 149, 220, 613, 940
AgsI TTSAA 5 cut(s) 457, 658, 717, 1246, 1304
AloI GAACNNNNNNTCC 2 cut(s) 380, 412
AluBI AGCT 4 cut(s) 709, 1016, 1129, 1240
AluI AGCT 4 cut(s) 709, 1016, 1129, 1240
Alw26I GTCTC 4 cut(s) 63, 114, 182, 557
AlwI GGATC 2 cut(s) 130, 668
Ama87I CYCGRG 1 cut(s) 305
AoxI GGCC 3 cut(s) 500, 697, 1104
ApeKI GCWGC 2 cut(s) 709, 803
ApoI RAATTY 1 cut(s) 526
ArsI GACNNNNNNTTYG 2 cut(s) 182, 214
AspLEI GCGC 3 cut(s) 252, 375, 385
AspS9I GGNCC 1 cut(s) 698
AsuC2I CCSGG 1 cut(s) 522
AsuHPI GGTGA 4 cut(s) 182, 361, 884, 1243
AsuII TTCGAA 1 cut(s) 201
AvaI CYCGRG 1 cut(s) 305
BaeI ACNNNNGTAYC 2 cut(s) 555, 588
BanII GRGCYC 1 cut(s) 306
BauI CACGAG 1 cut(s) 314
BbsI GAAGAC 2 cut(s) 190, 210
BbvCI CCTCAGC 2 cut(s) 440, 938
BbvI GCAGC 2 cut(s) 696, 815
BccI CCATC 5 cut(s) 35, 385, 551, 606, 1166
BceAI ACGGC 3 cut(s) 208, 712, 746
BcgI CGANNNNNNTGC 4 cut(s) 626, 660, 970, 1004
BcnI CCSGG 1 cut(s) 522
BcoDI GTCTC 4 cut(s) 63, 114, 182, 557
BfaI CTAG 2 cut(s) 609, 758
BfuAI ACCTGC 1 cut(s) 942
BglI GCCNNNNNGGC 1 cut(s) 488
BisI GCNGC 3 cut(s) 710, 804, 877
BlsI GCNGC 3 cut(s) 711, 805, 878
BmcAI AGTACT 1 cut(s) 888
Bme1390I CCNGG 1 cut(s) 522
BmeT110I CYCGRG 1 cut(s) 305
BmgT120I GGNCC 1 cut(s) 698
BmiI GGNNCC 1 cut(s) 597
BmrFI CCNGG 1 cut(s) 522
BmsI GCATC 3 cut(s) 136, 978, 1019
BpiI GAAGAC 2 cut(s) 190, 210
Bpu10I CCTNAGC 3 cut(s) 440, 938, 1062
Bpu14I TTCGAA 1 cut(s) 201
BpuMI CCSGG 1 cut(s) 522
BsaAI YACGTR 1 cut(s) 471
BsaHI GRCGYC 1 cut(s) 195
BsaI GGTCTC 2 cut(s) 114, 557
BsaJI CCNNGG 1 cut(s) 1204
BsaXI ACNNNNNCTCC 2 cut(s) 380, 410
Bsc4I CCNNNNNNNGG 4 cut(s) 149, 220, 613, 940
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 1 cut(s) 804
BseDI CCNNGG 1 cut(s) 1204
BseGI GGATG 1 cut(s) 1177
BseLI CCNNNNNNNGG 4 cut(s) 149, 220, 613, 940
BseMII CTCAG 4 cut(s) 245, 431, 929, 1076
BseNI ACTGG 1 cut(s) 804
BseXI GCAGC 2 cut(s) 696, 815
BseYI CCCAGC 2 cut(s) 581, 677
BsgI GTGCAG 1 cut(s) 1073
BshFI GGCC 3 cut(s) 502, 699, 1106
BsiHKCI CYCGRG 1 cut(s) 305
BsiSI CCGG 3 cut(s) 150, 521, 745
BslFI GGGAC 1 cut(s) 1085
BslI CCNNNNNNNGG 4 cut(s) 149, 220, 613, 940
BsmAI GTCTC 4 cut(s) 63, 114, 182, 557
BsmBI CGTCTC 1 cut(s) 63
BsmFI GGGAC 1 cut(s) 1085
BsnI GGCC 3 cut(s) 502, 699, 1106
Bso31I GGTCTC 2 cut(s) 114, 557
BsoBI CYCGRG 1 cut(s) 305
Bsp119I TTCGAA 1 cut(s) 201
Bsp1286I GDGCHC 1 cut(s) 306
Bsp143I GATC 2 cut(s) 135, 673
BspACI CCGC 7 cut(s) 270, 344, 437, 567, 876, 1148, 1184
BspANI GGCC 3 cut(s) 502, 699, 1106
BspCNI CTCAG 4 cut(s) 244, 432, 930, 1075
BspLI GGNNCC 1 cut(s) 597
BspMI ACCTGC 1 cut(s) 942
BspPI GGATC 2 cut(s) 130, 668
BspT104I TTCGAA 1 cut(s) 201
BspTNI GGTCTC 2 cut(s) 114, 557
BsrFI RCCGGY 1 cut(s) 149
BsrI ACTGG 1 cut(s) 804
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 1 cut(s) 1204
BssMI GATC 2 cut(s) 135, 673
BssNI GRCGYC 1 cut(s) 195
BssSI CACGAG 1 cut(s) 314
BssT1I CCWWGG 1 cut(s) 1204
Bst2BI CACGAG 1 cut(s) 314
Bst4CI ACNGT 5 cut(s) 108, 176, 542, 886, 1041
Bst6I CTCTTC 7 cut(s) 39, 81, 413, 717, 795, 1204, 1268
BstACI GRCGYC 1 cut(s) 195
BstBAI YACGTR 1 cut(s) 471
BstBI TTCGAA 1 cut(s) 201
BstC8I GCNNGC 3 cut(s) 151, 587, 918
BstDEI CTNAG 7 cut(s) 231, 276, 440, 600, 938, 1062, 1259
BstENI CCTNNNNNAGG 1 cut(s) 218
BstF5I GGATG 1 cut(s) 1177
BstHHI GCGC 3 cut(s) 252, 375, 385
BstKTI GATC 2 cut(s) 138, 676
BstMAI GTCTC 4 cut(s) 63, 114, 182, 557
BstMBI GATC 2 cut(s) 135, 673
BstMWI GCNNNNNNNGC 3 cut(s) 436, 488, 1103
BstNSI RCATGY 1 cut(s) 810
BstSCI CCNGG 1 cut(s) 520
BstV1I GCAGC 2 cut(s) 696, 815
BstV2I GAAGAC 2 cut(s) 190, 210
BstXI CCANNNNNNTGG 1 cut(s) 327
BsuRI GGCC 3 cut(s) 502, 699, 1106
BtgZI GCGATG 2 cut(s) 168, 570
BtsCI GGATG 1 cut(s) 1177
BtsI GCAGTG 1 cut(s) 1085
BtsIMutI CAGTG 3 cut(s) 547, 797, 1085
BveI ACCTGC 1 cut(s) 942
Cac8I GCNNGC 3 cut(s) 151, 587, 918
CfoI GCGC 3 cut(s) 252, 375, 385
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 698
CseI GACGC 2 cut(s) 184, 537
Csp6I GTAC 4 cut(s) 472, 670, 887, 1100
CspCI CAANNNNNGTGG 2 cut(s) 852, 887
CviAII CATG 1 cut(s) 807
CviQI GTAC 4 cut(s) 472, 670, 887, 1100
DdeI CTNAG 7 cut(s) 231, 276, 440, 600, 938, 1062, 1259
DpnI GATC 2 cut(s) 137, 675
DpnII GATC 2 cut(s) 135, 673
DrdI GACNNNNNNGTC 1 cut(s) 179
DseDI GACNNNNNNGTC 1 cut(s) 179
Eam1104I CTCTTC 7 cut(s) 39, 81, 413, 717, 795, 1204, 1268
EarI CTCTTC 7 cut(s) 39, 81, 413, 717, 795, 1204, 1268
EciI GGCGGA 2 cut(s) 359, 1163
Eco130I CCWWGG 1 cut(s) 1204
Eco147I AGGCCT 1 cut(s) 502
Eco24I GRGCYC 1 cut(s) 306
Eco31I GGTCTC 2 cut(s) 114, 557
Eco32I GATATC 1 cut(s) 856
Eco88I CYCGRG 1 cut(s) 305
EcoNI CCTNNNNNAGG 1 cut(s) 218
EcoRV GATATC 1 cut(s) 856
EcoT14I CCWWGG 1 cut(s) 1204
EcoT38I GRGCYC 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 1204
Esp3I CGTCTC 1 cut(s) 63
FaeI CATG 1 cut(s) 810
FaiI YATR 7 cut(s) 734, 808, 996, 1044, 1055, 1154, 1227
FaqI GGGAC 1 cut(s) 1085
FatI CATG 1 cut(s) 806
FauI CCCGC 1 cut(s) 263
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 3 cut(s) 710, 804, 877
FokI GGATG 1 cut(s) 1184
FriOI GRGCYC 1 cut(s) 306
Fsp4HI GCNGC 3 cut(s) 710, 804, 877
FspBI CTAG 2 cut(s) 609, 758
GlaI GCGC 3 cut(s) 251, 374, 384
GluI GCNGC 3 cut(s) 710, 804, 877
GsaI CCCAGC 2 cut(s) 585, 681
HaeIII GGCC 3 cut(s) 502, 699, 1106
HapII CCGG 3 cut(s) 150, 521, 745
HgaI GACGC 2 cut(s) 184, 537
HhaI GCGC 3 cut(s) 252, 375, 385
Hin1I GRCGYC 1 cut(s) 195
Hin1II CATG 1 cut(s) 810
Hin6I GCGC 3 cut(s) 250, 373, 383
HinP1I GCGC 3 cut(s) 250, 373, 383
HincII GTYRAC 2 cut(s) 451, 1179
HindII GTYRAC 2 cut(s) 451, 1179
HindIII AAGCTT 1 cut(s) 1127
HinfI GANTC 8 cut(s) 259, 290, 352, 633, 748, 830, 944, 1081
HpaI GTTAAC 1 cut(s) 1179
HpaII CCGG 3 cut(s) 150, 521, 745
HphI GGTGA 4 cut(s) 182, 361, 884, 1243
Hpy166II GTNNAC 5 cut(s) 184, 451, 622, 1080, 1179
Hpy188I TCNGA 4 cut(s) 295, 357, 535, 694
Hpy188III TCNNGA 5 cut(s) 512, 752, 851, 965, 1064
Hpy8I GTNNAC 5 cut(s) 184, 451, 622, 1080, 1179
Hpy99I CGWCG 3 cut(s) 136, 539, 698
HpyAV CCTTC 2 cut(s) 358, 968
HpyCH4III ACNGT 5 cut(s) 108, 176, 542, 886, 1041
HpyCH4IV ACGT 5 cut(s) 131, 470, 642, 1092, 1223
HpyCH4V TGCA 6 cut(s) 4, 863, 991, 1003, 1090, 1097
HpyF10VI GCNNNNNNNGC 3 cut(s) 436, 488, 1103
HpyF3I CTNAG 7 cut(s) 231, 276, 440, 600, 938, 1062, 1259
HpySE526I ACGT 5 cut(s) 131, 470, 642, 1092, 1223
Hsp92I GRCGYC 1 cut(s) 195
Hsp92II CATG 1 cut(s) 810
HspAI GCGC 3 cut(s) 250, 373, 383
KroI GCCGGC 1 cut(s) 149
KroNI GCCGGC 1 cut(s) 151
KspAI GTTAAC 1 cut(s) 1179
Kzo9I GATC 2 cut(s) 135, 673
LmnI GCTCC 4 cut(s) 247, 545, 601, 1021
Lsp1109I GCAGC 2 cut(s) 696, 815
LweI GCATC 3 cut(s) 136, 978, 1019
MaeI CTAG 2 cut(s) 609, 758
MaeII ACGT 5 cut(s) 131, 470, 642, 1092, 1223
MaeIII GTNAC 4 cut(s) 6, 170, 226, 1282
MalI GATC 2 cut(s) 137, 675
MboI GATC 2 cut(s) 135, 673
MfeI CAATTG 1 cut(s) 494
MhlI GDGCHC 1 cut(s) 306
MluCI AATT 3 cut(s) 432, 494, 526
MlyI GAGTC 1 cut(s) 1075
MmeI TCCRAC 2 cut(s) 558, 717
MroNI GCCGGC 1 cut(s) 149
MseI TTAA 1 cut(s) 1178
MslI CAYNNNNRTG 1 cut(s) 1157
MspA1I CMGCKG 2 cut(s) 439, 569
MspI CCGG 3 cut(s) 150, 521, 745
MspR9I CCNGG 1 cut(s) 522
MunI CAATTG 1 cut(s) 494
MwoI GCNNNNNNNGC 3 cut(s) 436, 488, 1103
NaeI GCCGGC 1 cut(s) 151
NciI CCSGG 1 cut(s) 522
NdeII GATC 2 cut(s) 135, 673
NgoMIV GCCGGC 1 cut(s) 149
NlaIII CATG 1 cut(s) 810
NlaIV GGNNCC 1 cut(s) 597
NmuCI GTSAC 3 cut(s) 6, 170, 226
NspI RCATGY 1 cut(s) 810
NspV TTCGAA 1 cut(s) 201
PceI AGGCCT 1 cut(s) 502
PcsI WCGNNNNNNNCGW 3 cut(s) 192, 198, 678
PdiI GCCGGC 1 cut(s) 151
PfeI GAWTC 7 cut(s) 259, 290, 352, 633, 748, 830, 944
PflFI GACNNNGTC 1 cut(s) 194
PkrI GCNGC 3 cut(s) 711, 805, 878
PleI GAGTC 1 cut(s) 1075
PpsI GAGTC 1 cut(s) 1075
Ppu21I YACGTR 1 cut(s) 471
PspFI CCCAGC 2 cut(s) 581, 677
PspN4I GGNNCC 1 cut(s) 597
PspPI GGNCC 1 cut(s) 698
PsyI GACNNNGTC 1 cut(s) 194
RsaI GTAC 4 cut(s) 473, 671, 888, 1101
RsaNI GTAC 4 cut(s) 472, 670, 887, 1100
RseI CAYNNNNRTG 1 cut(s) 1157
SaqAI TTAA 1 cut(s) 1178
SatI GCNGC 3 cut(s) 710, 804, 877
Sau3AI GATC 2 cut(s) 135, 673
Sau96I GGNCC 1 cut(s) 698
ScaI AGTACT 1 cut(s) 888
SchI GAGTC 1 cut(s) 1075
ScrFI CCNGG 1 cut(s) 522
SduI GDGCHC 1 cut(s) 306
SfaNI GCATC 3 cut(s) 136, 978, 1019
SfuI TTCGAA 1 cut(s) 201
SmiMI CAYNNNNRTG 1 cut(s) 1157
Sse9I AATT 3 cut(s) 432, 494, 526
SseBI AGGCCT 1 cut(s) 502
SsiI CCGC 7 cut(s) 270, 344, 437, 567, 876, 1148, 1184
SspMI CTAG 2 cut(s) 609, 758
StuI AGGCCT 1 cut(s) 502
StyD4I CCNGG 1 cut(s) 520
StyI CCWWGG 1 cut(s) 1204
TaaI ACNGT 5 cut(s) 108, 176, 542, 886, 1041
TaiI ACGT 5 cut(s) 134, 473, 645, 1095, 1226
TaqI TCGA 6 cut(s) 100, 134, 201, 513, 636, 703
TasI AATT 3 cut(s) 432, 494, 526
TatI WGTACW 2 cut(s) 886, 1099
TauI GCSGC 1 cut(s) 879
TfiI GAWTC 7 cut(s) 259, 290, 352, 633, 748, 830, 944
Tru1I TTAA 1 cut(s) 1178
Tru9I TTAA 1 cut(s) 1178
TscAI CASTG 3 cut(s) 547, 804, 1092
TseFI GTSAC 3 cut(s) 6, 170, 226
TseI GCWGC 2 cut(s) 709, 803
Tsp45I GTSAC 3 cut(s) 6, 170, 226
TspDTI ATGAA 4 cut(s) 151, 303, 1250, 1308
TspGWI ACGGA 2 cut(s) 45, 639
TspRI CASTG 3 cut(s) 547, 804, 1092
Tth111I GACNNNGTC 1 cut(s) 194
XagI CCTNNNNNAGG 1 cut(s) 218
XapI RAATTY 1 cut(s) 526
XceI RCATGY 1 cut(s) 810
XcmI CCANNNNNNNNNTGG 1 cut(s) 589
XmiI GTMKAC 1 cut(s) 183
XspI CTAG 2 cut(s) 609, 758
ZrmI AGTACT 1 cut(s) 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.