pycom15g21070

Ankyrin repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001128
Physical Location & Seq
Reverse (-)
15090971 .. 15093103
2133 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g21070.6

Sequence Viewer

Length: 1287 bp
ATGGCGAGTGCAAAGACCATGCCACTCTCTCTAAACCCCCAATCCACCTTCATCCTCGCCAAACCCCTCTTTTTCCCCACCACACCTCGCAAACTATCGGCGCCTGCTACCCTAAAGTTCCCGCGAAAGCTCCATGGCCTCTATCCCTCTTTCACCTCCTCCTACTCCGTCCAAACCGACCATTTTGACGAGGACCATGTAATAGGCGACTGCGTCGTGTTCGAAGAAGGCGTGTTCGACGACCCCTACCTCCAAAAAGATACCAAAACCGACCGTATCAGACCCGAGAAAGCGAAACCCAGAAGAGATGGTTCCGAAATCGGGTCGGAGAATTTGGTTCCGGACCAGTGGCGGGAGGTGCAGGCGGAGATTAACATAACGAAGAAAGAGCGGCGGAAGATTGCGCAGGAGTTGCAGTTCGGGACGAGGGTGGAGAAGCAGAAGAAGGGGCTGGAGCCGATTAGGGATTTGAATTTGGAGGAGTACTTGGCGTATAGGGAGGCGAAATTGAACCAATTGAAGCCACTTGTTTTGGATGATGCTTCTGGTTTAGCTCCGGCGGAGGAGTTGAAGGAGAATGAAGGGGATGCTAGGAAATTGAGTGAGAGAGTGGCGCCGAAGAATCCGAGGTGGGCGGTTTATGGGAAGGGATTGGAGGATGTTGCAGATTACTTTAACAGTGGGAATTATGAGCCTGGTGCTAAGAAATCTGAAGGACCTCGAAAGTTATTTTCGAAAGAGGAAAAAGCTCTCCTTAATAAGCGAAAACCTGATATAGCTGCTGCTACCTCTAGCAAATGGCTACCCCTGCACACTCTTGCTGCATCTGGAGAATTTTACCTTATGGATGCTTTATTGAAACATAATGCTGATATCAATGTCGTGGATAAGCACGGTTGGAGTGCTCTTCACAAAGCAATTCTTGGTAAAAACCAGGCCATCACGAACTATCTTTTGAGAGAATCAGCTAATCCATTTGTTCGTGATAAAGATGGCGGCACATTGATGCATTATGCTGTTCGAACAGCTTCCAGTCAAGCGATCAAAATTCTTCTTTTATATAATGTTGATATAAACCTTCAGGACAACGATGGTTGGACGCCGTTACACCTAGCTGTTCAAGGCCGAAGAACAGATGTTGTGAGGCTTTTGCTGATTAAAGGAGCGAATAAGACATTGAAGAACAAGGATGGTTTATCCCCTCTTGACCTTTGCCTCTACTCTGGTCAAGACACGAGGACTTACGAGCTAATTAAGCTGTTGAAGCTTCTTCCAAAGTCGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 RRM_1

Protein Analysis

429

Amino Acids

48.26

Weight (kDa)

9.21

Isoelectric Point (pI)

38.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 405
AccB1I GGYRCC 2 cut(s) 100, 613
AccBSI CCGCTC 1 cut(s) 391
AccII CGCG 2 cut(s) 124, 1282
AccIII TCCGGA 1 cut(s) 340
AciI CCGC 8 cut(s) 122, 352, 365, 391, 394, 560, 635, 996
AcsI RAATTY 4 cut(s) 331, 472, 833, 1047
AcuI CTGAAG 2 cut(s) 732, 1064
AcyI GRCGYC 3 cut(s) 101, 614, 1100
AfaI GTAC 1 cut(s) 485
AfiI CCNNNNNNNGG 2 cut(s) 321, 352
AgsI TTSAA 8 cut(s) 472, 511, 520, 571, 859, 1121, 1180, 1264
AjnI CCWGG 2 cut(s) 694, 933
AloI GAACNNNNNNTCC 2 cut(s) 401, 433
Alw21I GWGCWC 1 cut(s) 907
Ama87I CYCGRG 1 cut(s) 284
Aor13HI TCCGGA 1 cut(s) 340
AoxI GGCC 3 cut(s) 136, 936, 1123
ApeKI GCWGC 3 cut(s) 779, 782, 821
ApoI RAATTY 4 cut(s) 331, 472, 833, 1047
Asp700I GAANNNNTTC 1 cut(s) 1027
AspLEI GCGC 3 cut(s) 103, 406, 616
AspS9I GGNCC 3 cut(s) 193, 343, 716
AsuHPI GGTGA 1 cut(s) 145
AsuII TTCGAA 3 cut(s) 222, 734, 1021
AvaI CYCGRG 1 cut(s) 284
AvaII GGWCC 3 cut(s) 193, 343, 716
BanI GGYRCC 2 cut(s) 100, 613
BauI CACGAG 1 cut(s) 1234
Bbv12I GWGCWC 1 cut(s) 907
BbvI GCAGC 3 cut(s) 766, 769, 808
BccI CCATC 5 cut(s) 302, 947, 986, 1085, 1184
BceAI ACGGC 1 cut(s) 1087
BciT130I CCWGG 2 cut(s) 696, 935
BfaI CTAG 3 cut(s) 591, 792, 1112
BfoI RGCGCY 2 cut(s) 104, 617
BisI GCNGC 5 cut(s) 392, 780, 783, 822, 997
BlsI GCNGC 5 cut(s) 393, 781, 784, 823, 998
BmcAI AGTACT 1 cut(s) 485
Bme1390I CCNGG 2 cut(s) 696, 935
Bme18I GGWCC 3 cut(s) 193, 343, 716
BmeT110I CYCGRG 1 cut(s) 284
BmgT120I GGNCC 3 cut(s) 193, 343, 716
BmiI GGNNCC 5 cut(s) 102, 313, 339, 456, 615
BmrFI CCNGG 2 cut(s) 696, 935
BmsI GCATC 5 cut(s) 529, 577, 833, 838, 996
BpmI CTGGAG 2 cut(s) 473, 849
Bpu14I TTCGAA 3 cut(s) 222, 734, 1021
BsaHI GRCGYC 3 cut(s) 101, 614, 1100
BsaJI CCNNGG 2 cut(s) 133, 626
BsaWI WCCGGW 1 cut(s) 340
BsaXI ACNNNNNCTCC 2 cut(s) 401, 431
Bsc4I CCNNNNNNNGG 2 cut(s) 321, 352
Bse1I ACTGG 2 cut(s) 346, 1032
BseAI TCCGGA 1 cut(s) 340
BseBI CCWGG 2 cut(s) 696, 935
BseDI CCNNGG 2 cut(s) 133, 626
BseGI GGATG 6 cut(s) 51, 541, 592, 664, 853, 1195
BseLI CCNNNNNNNGG 2 cut(s) 321, 352
BseNI ACTGG 2 cut(s) 346, 1032
BseRI GAGGAG 3 cut(s) 148, 494, 578
BseXI GCAGC 3 cut(s) 766, 769, 808
BsgI GTGCAG 2 cut(s) 380, 794
Bsh1236I CGCG 2 cut(s) 124, 1282
Bsh1285I CGRYCG 1 cut(s) 274
BshFI GGCC 3 cut(s) 138, 938, 1125
BshNI GGYRCC 2 cut(s) 100, 613
BsiEI CGRYCG 1 cut(s) 274
BsiHKAI GWGCWC 1 cut(s) 907
BsiHKCI CYCGRG 1 cut(s) 284
BsiSI CCGG 2 cut(s) 341, 557
BslFI GGGAC 1 cut(s) 436
BslI CCNNNNNNNGG 2 cut(s) 321, 352
BsmFI GGGAC 1 cut(s) 436
BsnI GGCC 3 cut(s) 138, 938, 1125
BsoBI CYCGRG 1 cut(s) 284
Bsp119I TTCGAA 3 cut(s) 222, 734, 1021
Bsp1286I GDGCHC 1 cut(s) 907
Bsp13I TCCGGA 1 cut(s) 340
Bsp143I GATC 1 cut(s) 1041
Bsp19I CCATGG 1 cut(s) 133
BspACI CCGC 8 cut(s) 122, 352, 365, 391, 394, 560, 635, 996
BspANI GGCC 3 cut(s) 138, 938, 1125
BspEI TCCGGA 1 cut(s) 340
BspFNI CGCG 2 cut(s) 124, 1282
BspLI GGNNCC 5 cut(s) 102, 313, 339, 456, 615
BspQI GCTCTTC 1 cut(s) 912
BspT104I TTCGAA 3 cut(s) 222, 734, 1021
BspT107I GGYRCC 2 cut(s) 100, 613
BsrBI CCGCTC 1 cut(s) 391
BsrI ACTGG 2 cut(s) 346, 1032
BssECI CCNNGG 2 cut(s) 133, 626
BssMI GATC 1 cut(s) 1041
BssNI GRCGYC 3 cut(s) 101, 614, 1100
BssSI CACGAG 1 cut(s) 1234
BssT1I CCWWGG 1 cut(s) 133
Bst2BI CACGAG 1 cut(s) 1234
Bst2UI CCWGG 2 cut(s) 696, 935
Bst4CI ACNGT 3 cut(s) 275, 680, 896
Bst6I CTCTTC 2 cut(s) 298, 912
BstACI GRCGYC 3 cut(s) 101, 614, 1100
BstAPI GCANNNNNTGC 1 cut(s) 412
BstBI TTCGAA 3 cut(s) 222, 734, 1021
BstC8I GCNNGC 2 cut(s) 105, 363
BstDEI CTNAG 1 cut(s) 702
BstDSI CCRYGG 1 cut(s) 133
BstF5I GGATG 6 cut(s) 51, 541, 592, 664, 853, 1195
BstFNI CGCG 2 cut(s) 124, 1282
BstH2I RGCGCY 2 cut(s) 104, 617
BstHHI GCGC 3 cut(s) 103, 406, 616
BstKTI GATC 1 cut(s) 1044
BstMBI GATC 1 cut(s) 1041
BstMCI CGRYCG 1 cut(s) 274
BstMWI GCNNNNNNNGC 5 cut(s) 358, 412, 808, 1255, 1264
BstNI CCWGG 2 cut(s) 696, 935
BstSCI CCNGG 2 cut(s) 694, 933
BstUI CGCG 2 cut(s) 124, 1282
BstV1I GCAGC 3 cut(s) 766, 769, 808
BsuRI GGCC 3 cut(s) 138, 938, 1125
BtgI CCRYGG 1 cut(s) 133
BtsCI GGATG 6 cut(s) 51, 541, 592, 664, 853, 1195
BtsIMutI CAGTG 2 cut(s) 353, 685
Cac8I GCNNGC 2 cut(s) 105, 363
CfoI GCGC 3 cut(s) 103, 406, 616
Cfr13I GGNCC 3 cut(s) 193, 343, 716
CseI GACGC 2 cut(s) 202, 1108
Csp6I GTAC 1 cut(s) 484
CspCI CAANNNNNGTGG 2 cut(s) 513, 548
CviAII CATG 3 cut(s) 19, 134, 197
CviQI GTAC 1 cut(s) 484
DdeI CTNAG 1 cut(s) 702
DinI GGCGCC 2 cut(s) 102, 615
DpnI GATC 1 cut(s) 1043
DpnII GATC 1 cut(s) 1041
Eam1104I CTCTTC 2 cut(s) 298, 912
EarI CTCTTC 2 cut(s) 298, 912
EciI GGCGGA 3 cut(s) 380, 409, 575
Eco130I CCWWGG 1 cut(s) 133
Eco32I GATATC 1 cut(s) 874
Eco47I GGWCC 3 cut(s) 193, 343, 716
Eco57I CTGAAG 2 cut(s) 732, 1064
Eco88I CYCGRG 1 cut(s) 284
EcoO109I RGGNCCY 1 cut(s) 716
EcoRII CCWGG 2 cut(s) 694, 933
EcoRV GATATC 1 cut(s) 874
EcoT14I CCWWGG 1 cut(s) 133
EcoT22I ATGCAT 1 cut(s) 1011
EgeI GGCGCC 2 cut(s) 102, 615
EheI GGCGCC 2 cut(s) 102, 615
ErhI CCWWGG 1 cut(s) 133
FaeI CATG 3 cut(s) 22, 137, 200
FalI AAGNNNNNCTT 4 cut(s) 738, 770, 906, 938
FaqI GGGAC 1 cut(s) 436
FatI CATG 3 cut(s) 18, 133, 196
FauI CCCGC 2 cut(s) 129, 345
Fnu4HI GCNGC 5 cut(s) 392, 780, 783, 822, 997
FokI GGATG 6 cut(s) 38, 548, 599, 671, 860, 1202
Fsp4HI GCNGC 5 cut(s) 392, 780, 783, 822, 997
FspBI CTAG 3 cut(s) 591, 792, 1112
FspI TGCGCA 1 cut(s) 405
GlaI GCGC 3 cut(s) 102, 405, 615
GluI GCNGC 5 cut(s) 392, 780, 783, 822, 997
GsuI CTGGAG 2 cut(s) 473, 849
HaeII RGCGCY 2 cut(s) 104, 617
HaeIII GGCC 3 cut(s) 138, 938, 1125
HapII CCGG 2 cut(s) 341, 557
HgaI GACGC 2 cut(s) 202, 1108
HhaI GCGC 3 cut(s) 103, 406, 616
Hin1I GRCGYC 3 cut(s) 101, 614, 1100
Hin1II CATG 3 cut(s) 22, 137, 200
Hin6I GCGC 3 cut(s) 101, 404, 614
HinP1I GCGC 3 cut(s) 101, 404, 614
HindIII AAGCTT 1 cut(s) 1265
HinfI GANTC 2 cut(s) 622, 962
HpaII CCGG 2 cut(s) 341, 557
HphI GGTGA 1 cut(s) 145
Hpy188I TCNGA 5 cut(s) 281, 316, 328, 627, 712
Hpy188III TCNNGA 8 cut(s) 341, 421, 828, 943, 983, 1082, 1205, 1229
Hpy99I CGWCG 2 cut(s) 218, 242
HpyAV CCTTC 8 cut(s) 58, 221, 439, 565, 575, 640, 707, 1088
HpyCH4III ACNGT 3 cut(s) 275, 680, 896
HpyCH4V TGCA 7 cut(s) 11, 361, 415, 665, 811, 824, 1009
HpyF10VI GCNNNNNNNGC 5 cut(s) 358, 412, 808, 1255, 1264
HpyF3I CTNAG 1 cut(s) 702
Hsp92I GRCGYC 3 cut(s) 101, 614, 1100
Hsp92II CATG 3 cut(s) 22, 137, 200
HspAI GCGC 3 cut(s) 101, 404, 614
KasI GGCGCC 2 cut(s) 100, 613
Kpn2I TCCGGA 1 cut(s) 340
Kzo9I GATC 1 cut(s) 1041
LguI GCTCTTC 1 cut(s) 912
LmnI GCTCC 4 cut(s) 135, 454, 559, 1163
Lsp1109I GCAGC 3 cut(s) 766, 769, 808
LweI GCATC 5 cut(s) 529, 577, 833, 838, 996
MaeI CTAG 3 cut(s) 591, 792, 1112
MaeIII GTNAC 1 cut(s) 1104
MalI GATC 1 cut(s) 1043
MbiI CCGCTC 1 cut(s) 391
MboI GATC 1 cut(s) 1041
MfeI CAATTG 1 cut(s) 515
MhlI GDGCHC 1 cut(s) 907
Mly113I GGCGCC 2 cut(s) 101, 614
MmeI TCCRAC 3 cut(s) 306, 878, 1076
Mph1103I ATGCAT 1 cut(s) 1011
MroI TCCGGA 1 cut(s) 340
MroXI GAANNNNTTC 1 cut(s) 1027
MseI TTAA 6 cut(s) 372, 675, 756, 1158, 1254, 1285
MslI CAYNNNNRTG 1 cut(s) 1004
MspI CCGG 2 cut(s) 341, 557
MspR9I CCNGG 2 cut(s) 696, 935
MunI CAATTG 1 cut(s) 515
MvaI CCWGG 2 cut(s) 696, 935
MvnI CGCG 2 cut(s) 124, 1282
MwoI GCNNNNNNNGC 5 cut(s) 358, 412, 808, 1255, 1264
NarI GGCGCC 2 cut(s) 101, 614
NcoI CCATGG 1 cut(s) 133
NdeII GATC 1 cut(s) 1041
NlaIII CATG 3 cut(s) 22, 137, 200
NlaIV GGNNCC 5 cut(s) 102, 313, 339, 456, 615
NsbI TGCGCA 1 cut(s) 405
NsiI ATGCAT 1 cut(s) 1011
NspV TTCGAA 3 cut(s) 222, 734, 1021
PciSI GCTCTTC 1 cut(s) 912
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PdmI GAANNNNTTC 1 cut(s) 1027
PfeI GAWTC 2 cut(s) 622, 962
PflFI GACNNNGTC 1 cut(s) 212
PkrI GCNGC 5 cut(s) 393, 781, 784, 823, 998
PluTI GGCGCC 2 cut(s) 104, 617
PpuMI RGGWCCY 1 cut(s) 716
Psp5II RGGWCCY 1 cut(s) 716
Psp6I CCWGG 2 cut(s) 694, 933
PspGI CCWGG 2 cut(s) 694, 933
PspN4I GGNNCC 5 cut(s) 102, 313, 339, 456, 615
PspPI GGNCC 3 cut(s) 193, 343, 716
PspPPI RGGWCCY 1 cut(s) 716
PsyI GACNNNGTC 1 cut(s) 212
RsaI GTAC 1 cut(s) 485
RsaNI GTAC 1 cut(s) 484
RseI CAYNNNNRTG 1 cut(s) 1004
SapI GCTCTTC 1 cut(s) 912
SaqAI TTAA 6 cut(s) 372, 675, 756, 1158, 1254, 1285
SatI GCNGC 5 cut(s) 392, 780, 783, 822, 997
Sau3AI GATC 1 cut(s) 1041
Sau96I GGNCC 3 cut(s) 193, 343, 716
ScaI AGTACT 1 cut(s) 485
ScrFI CCNGG 2 cut(s) 696, 935
SduI GDGCHC 1 cut(s) 907
SfaNI GCATC 5 cut(s) 529, 577, 833, 838, 996
SfoI GGCGCC 2 cut(s) 102, 615
SfuI TTCGAA 3 cut(s) 222, 734, 1021
SinI GGWCC 3 cut(s) 193, 343, 716
SmiMI CAYNNNNRTG 1 cut(s) 1004
SsiI CCGC 8 cut(s) 122, 352, 365, 391, 394, 560, 635, 996
SspDI GGCGCC 2 cut(s) 100, 613
SspMI CTAG 3 cut(s) 591, 792, 1112
StyD4I CCNGG 2 cut(s) 694, 933
StyI CCWWGG 1 cut(s) 133
TaaI ACNGT 3 cut(s) 275, 680, 896
TaqI TCGA 5 cut(s) 222, 237, 721, 734, 1021
TatI WGTACW 1 cut(s) 483
TauI GCSGC 2 cut(s) 394, 999
TfiI GAWTC 2 cut(s) 622, 962
Tru1I TTAA 6 cut(s) 372, 675, 756, 1158, 1254, 1285
Tru9I TTAA 6 cut(s) 372, 675, 756, 1158, 1254, 1285
TscAI CASTG 2 cut(s) 353, 685
TseI GCWGC 3 cut(s) 779, 782, 821
TspDTI ATGAA 2 cut(s) 40, 594
TspGWI ACGGA 1 cut(s) 157
TspRI CASTG 2 cut(s) 353, 685
Tth111I GACNNNGTC 1 cut(s) 212
VpaK11BI GGWCC 3 cut(s) 193, 343, 716
XapI RAATTY 4 cut(s) 331, 472, 833, 1047
XmnI GAANNNNTTC 1 cut(s) 1027
XspI CTAG 3 cut(s) 591, 792, 1112
ZrmI AGTACT 1 cut(s) 485
Zsp2I ATGCAT 1 cut(s) 1011
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.