Rroxscaffold_2G00144120

Ankyrin repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001128
Physical Location & Seq
Forward (+)
82105253 .. 82107778
2526 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00144120.1

Sequence Viewer

Length: 1089 bp
ATGTCACTCTCTTCAGCTCTAAACCCCCAAACCTCCAAGCTCTTCTCTCTCCTCCCCACCCCATCTCTCTCTTTCACACCCCACAAGCTCTCACCACCCACCACCCTGAAATTCTCATCCAAACTCCACACCCTCTCACCCTCCTTCAACTCCTCTCACTCCACCTTCGAAGAAGACCACGTCATCGGCGACTGCGTCGTCTTCGAAGAGGGCGTGTTCGATGACCCGTATCTTCAAAACGACACCGAAATTGAACGTTTCAACCCGGAAAAACCCAGGCCCAGAAAACGGATTGAAATTGAGACTGAGAATTTGGTCCCGGATAAATGGAAGGAGGTACAGGCCACAGTGAACATAACAAAGAAAGAGAGGAGGAAGATTGCGCAGGAGTTGGAGTTCGGTTCGAGGGTTGAGAAGAACAAGAGGGGGTTGGAGCCCATAAGGAATTTGAATTTGGAGGAGTACAAGGCGTATAGAGAGGCCAAGTTGAACCAGCTGAAGCCACTGGTTTTGGATAGGCCTGAGAAGTTTAAGGAGGAGGATGAAGAGTGTGAAGGTGGTGATGGGTTTGTGAGTGAGAGAGTGGCGCCTAAGAATCCCAGGTGGGCTGTTTATGGGAAGGGTTTGGACGATGTTACTGAGTTTTTAAACAGTGGGCATTATGAGCCTGGTGTGAAGAAATCTGAAGGACCCCGGCCTTTGTTTACGAAAGAGGAAAAGCTGATGCTGACTAGGCGAAAACCTGATATAGCTGCTGCCACTTCTAGTAAATGGCTGCCCCTTCACACTCTTGCCGCATCAGGAGAATATTACCTTATGGACGCTTTACTGAAACATGAAGCTGATATCAATGCTGTGGATAAGGATGATTGGACTGTTCTTCACAAAGCAATTATTGGTAAAAACCAGGCCATTACAAACTATCTTCTCAGAGAATCGGCTAATCCATTTGTTCGTGATAAAGATGGTGCTACCTTGATGCATTATGCTGTCCGGACAGCTTCAAGTCAAGCAATTAAGATTCTTCTGTTATACAACGTTGATATAAACCTTCAGGACAATGTATGGATTAAATTTTTCCATTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 RRM_1

Protein Analysis

362

Amino Acids

41.38

Weight (kDa)

6.4

Isoelectric Point (pI)

48.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 261 - 353 9.2e-14 Ankyrin repeats (3 copies)
Ank_4 PF13637 261 - 310 1.9e-07 Ankyrin repeats (many copies)
Ank_5 PF13857 276 - 330 3.4e-06 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 197
Acc16I TGCGCA 1 cut(s) 384
AccB1I GGYRCC 1 cut(s) 586
AccIII TCCGGA 1 cut(s) 993
AciI CCGC 1 cut(s) 795
AclI AACGTT 2 cut(s) 256, 1038
AcsI RAATTY 5 cut(s) 110, 310, 445, 451, 1073
AcuI CTGAAG 3 cut(s) 518, 705, 1037
AcyI GRCGYC 1 cut(s) 587
AfaI GTAC 2 cut(s) 339, 464
AfiI CCNNNNNNNGG 1 cut(s) 288
AgsI TTSAA 8 cut(s) 148, 236, 254, 262, 296, 451, 490, 1005
AjiI CACGTC 1 cut(s) 181
AjnI CCWGG 4 cut(s) 275, 599, 667, 906
AjuI GAANNNNNNNTTGG 2 cut(s) 437, 469
AloI GAACNNNNNNTCC 2 cut(s) 380, 412
AluBI AGCT 8 cut(s) 17, 40, 88, 496, 721, 752, 842, 1001
AluI AGCT 8 cut(s) 17, 40, 88, 496, 721, 752, 842, 1001
Alw26I GTCTC 1 cut(s) 296
Aor13HI TCCGGA 1 cut(s) 993
AoxI GGCC 6 cut(s) 278, 342, 480, 518, 695, 909
ApeKI GCWGC 3 cut(s) 752, 755, 775
ApoI RAATTY 5 cut(s) 110, 310, 445, 451, 1073
ArsI GACNNNNNNTTYG 2 cut(s) 295, 327
AspLEI GCGC 2 cut(s) 385, 589
AspS9I GGNCC 3 cut(s) 279, 316, 689
AsuC2I CCSGG 3 cut(s) 266, 320, 694
AsuHPI GGTGA 3 cut(s) 84, 129, 572
AsuII TTCGAA 2 cut(s) 168, 204
AvaII GGWCC 2 cut(s) 316, 689
BanI GGYRCC 1 cut(s) 586
BanII GRGCYC 1 cut(s) 438
BbsI GAAGAC 2 cut(s) 180, 193
BbvI GCAGC 3 cut(s) 739, 742, 762
BccI CCATC 3 cut(s) 70, 557, 959
BciT130I CCWGG 4 cut(s) 277, 601, 669, 908
BcnI CCSGG 3 cut(s) 266, 320, 694
BcoDI GTCTC 1 cut(s) 296
BfaI CTAG 2 cut(s) 732, 765
BfoI RGCGCY 1 cut(s) 590
BisI GCNGC 4 cut(s) 753, 756, 776, 795
BlsI GCNGC 4 cut(s) 754, 757, 777, 796
Bme1390I CCNGG 7 cut(s) 266, 277, 320, 601, 669, 694, 908
Bme18I GGWCC 2 cut(s) 316, 689
BmgBI CACGTC 1 cut(s) 181
BmgT120I GGNCC 3 cut(s) 279, 316, 689
BmiI GGNNCC 4 cut(s) 318, 435, 588, 691
BmrFI CCNGG 7 cut(s) 266, 277, 320, 601, 669, 694, 908
BmsI GCATC 3 cut(s) 714, 806, 969
BpiI GAAGAC 2 cut(s) 180, 193
Bpu14I TTCGAA 2 cut(s) 168, 204
BpuMI CCSGG 3 cut(s) 266, 320, 694
BsaHI GRCGYC 1 cut(s) 587
BsaJI CCNNGG 3 cut(s) 275, 599, 692
BsaWI WCCGGW 1 cut(s) 993
BsaXI ACNNNNNCTCC 2 cut(s) 380, 410
Bsc4I CCNNNNNNNGG 1 cut(s) 288
Bse1I ACTGG 1 cut(s) 510
BseAI TCCGGA 1 cut(s) 993
BseBI CCWGG 4 cut(s) 277, 601, 669, 908
BseDI CCNNGG 3 cut(s) 275, 599, 692
BseGI GGATG 3 cut(s) 116, 547, 871
BseLI CCNNNNNNNGG 1 cut(s) 288
BseMII CTCAG 4 cut(s) 297, 513, 630, 943
BseNI ACTGG 1 cut(s) 510
BseRI GAGGAG 5 cut(s) 41, 142, 385, 473, 551
BseXI GCAGC 3 cut(s) 739, 742, 762
BshFI GGCC 6 cut(s) 280, 344, 482, 520, 697, 911
BshNI GGYRCC 1 cut(s) 586
BsiSI CCGG 4 cut(s) 266, 320, 694, 994
BslFI GGGAC 1 cut(s) 302
BslI CCNNNNNNNGG 1 cut(s) 288
BsmAI GTCTC 1 cut(s) 296
BsmFI GGGAC 1 cut(s) 302
BsnI GGCC 6 cut(s) 280, 344, 482, 520, 697, 911
Bsp119I TTCGAA 2 cut(s) 168, 204
Bsp1286I GDGCHC 1 cut(s) 438
Bsp13I TCCGGA 1 cut(s) 993
BspACI CCGC 1 cut(s) 795
BspANI GGCC 6 cut(s) 280, 344, 482, 520, 697, 911
BspCNI CTCAG 4 cut(s) 298, 514, 631, 942
BspEI TCCGGA 1 cut(s) 993
BspLI GGNNCC 4 cut(s) 318, 435, 588, 691
BspQI GCTCTTC 1 cut(s) 47
BspT104I TTCGAA 2 cut(s) 168, 204
BspT107I GGYRCC 1 cut(s) 586
BsrI ACTGG 1 cut(s) 510
BssECI CCNNGG 3 cut(s) 275, 599, 692
BssNI GRCGYC 1 cut(s) 587
Bst2UI CCWGG 4 cut(s) 277, 601, 669, 908
Bst4CI ACNGT 3 cut(s) 349, 653, 877
Bst6I CTCTTC 4 cut(s) 16, 47, 201, 540
BstACI GRCGYC 1 cut(s) 587
BstBI TTCGAA 2 cut(s) 168, 204
BstDEI CTNAG 5 cut(s) 306, 522, 591, 639, 929
BstF5I GGATG 3 cut(s) 116, 547, 871
BstH2I RGCGCY 1 cut(s) 590
BstHHI GCGC 2 cut(s) 385, 589
BstMAI GTCTC 1 cut(s) 296
BstMWI GCNNNNNNNGC 2 cut(s) 664, 733
BstNI CCWGG 4 cut(s) 277, 601, 669, 908
BstSCI CCNGG 7 cut(s) 264, 275, 318, 599, 667, 692, 906
BstV1I GCAGC 3 cut(s) 739, 742, 762
BstV2I GAAGAC 2 cut(s) 180, 193
BsuRI GGCC 6 cut(s) 280, 344, 482, 520, 697, 911
BtrI CACGTC 1 cut(s) 181
BtsCI GGATG 3 cut(s) 116, 547, 871
BtsIMutI CAGTG 3 cut(s) 354, 503, 658
CfoI GCGC 2 cut(s) 385, 589
Cfr13I GGNCC 3 cut(s) 279, 316, 689
CseI GACGC 2 cut(s) 184, 830
Csp6I GTAC 2 cut(s) 338, 463
CspCI CAANNNNNGTGG 2 cut(s) 492, 527
CviAII CATG 1 cut(s) 836
CviQI GTAC 2 cut(s) 338, 463
DdeI CTNAG 5 cut(s) 306, 522, 591, 639, 929
DinI GGCGCC 1 cut(s) 588
DraI TTTAAA 1 cut(s) 648
DrdI GACNNNNNNGTC 1 cut(s) 197
DseDI GACNNNNNNGTC 1 cut(s) 197
Eam1104I CTCTTC 4 cut(s) 16, 47, 201, 540
EarI CTCTTC 4 cut(s) 16, 47, 201, 540
Eco147I AGGCCT 1 cut(s) 520
Eco24I GRGCYC 1 cut(s) 438
Eco32I GATATC 1 cut(s) 847
Eco47I GGWCC 2 cut(s) 316, 689
Eco57I CTGAAG 3 cut(s) 518, 705, 1037
EcoO109I RGGNCCY 1 cut(s) 689
EcoRII CCWGG 4 cut(s) 275, 599, 667, 906
EcoRV GATATC 1 cut(s) 847
EcoT22I ATGCAT 1 cut(s) 984
EcoT38I GRGCYC 1 cut(s) 438
EgeI GGCGCC 1 cut(s) 588
EheI GGCGCC 1 cut(s) 588
FaeI CATG 1 cut(s) 839
FaqI GGGAC 1 cut(s) 302
FatI CATG 1 cut(s) 835
Fnu4HI GCNGC 4 cut(s) 753, 756, 776, 795
FokI GGATG 3 cut(s) 103, 554, 878
FriOI GRGCYC 1 cut(s) 438
Fsp4HI GCNGC 4 cut(s) 753, 756, 776, 795
FspBI CTAG 2 cut(s) 732, 765
FspI TGCGCA 1 cut(s) 384
GlaI GCGC 2 cut(s) 384, 588
GluI GCNGC 4 cut(s) 753, 756, 776, 795
HaeII RGCGCY 1 cut(s) 590
HaeIII GGCC 6 cut(s) 280, 344, 482, 520, 697, 911
HapII CCGG 4 cut(s) 266, 320, 694, 994
HgaI GACGC 2 cut(s) 184, 830
HhaI GCGC 2 cut(s) 385, 589
Hin1I GRCGYC 1 cut(s) 587
Hin1II CATG 1 cut(s) 839
Hin6I GCGC 2 cut(s) 383, 587
HinP1I GCGC 2 cut(s) 383, 587
HinfI GANTC 3 cut(s) 595, 935, 1021
HpaII CCGG 4 cut(s) 266, 320, 694, 994
HphI GGTGA 3 cut(s) 84, 129, 572
Hpy166II GTNNAC 2 cut(s) 352, 705
Hpy188I TCNGA 2 cut(s) 685, 932
Hpy188III TCNNGA 5 cut(s) 801, 956, 994, 1055, 1086
Hpy8I GTNNAC 2 cut(s) 352, 705
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 8 cut(s) 154, 175, 325, 548, 613, 680, 791, 1061
HpyCH4III ACNGT 3 cut(s) 349, 653, 877
HpyCH4IV ACGT 3 cut(s) 180, 256, 1038
HpyCH4V TGCA 1 cut(s) 982
HpyF10VI GCNNNNNNNGC 2 cut(s) 664, 733
HpyF3I CTNAG 5 cut(s) 306, 522, 591, 639, 929
HpySE526I ACGT 3 cut(s) 180, 256, 1038
Hsp92I GRCGYC 1 cut(s) 587
Hsp92II CATG 1 cut(s) 839
HspAI GCGC 2 cut(s) 383, 587
KasI GGCGCC 1 cut(s) 586
Kpn2I TCCGGA 1 cut(s) 993
LguI GCTCTTC 1 cut(s) 47
LmnI GCTCC 1 cut(s) 433
Lsp1109I GCAGC 3 cut(s) 739, 742, 762
LweI GCATC 3 cut(s) 714, 806, 969
MaeI CTAG 2 cut(s) 732, 765
MaeII ACGT 3 cut(s) 180, 256, 1038
MaeIII GTNAC 2 cut(s) 3, 634
MhlI GDGCHC 1 cut(s) 438
MluCI AATT 9 cut(s) 110, 249, 297, 310, 445, 451, 891, 1014, 1073
Mly113I GGCGCC 1 cut(s) 587
MmeI TCCRAC 2 cut(s) 372, 411
Mph1103I ATGCAT 1 cut(s) 984
MroI TCCGGA 1 cut(s) 993
MseI TTAA 4 cut(s) 531, 647, 1017, 1071
MspA1I CMGCKG 1 cut(s) 496
MspI CCGG 4 cut(s) 266, 320, 694, 994
MspR9I CCNGG 7 cut(s) 266, 277, 320, 601, 669, 694, 908
MvaI CCWGG 4 cut(s) 277, 601, 669, 908
MwoI GCNNNNNNNGC 2 cut(s) 664, 733
NarI GGCGCC 1 cut(s) 587
NciI CCSGG 3 cut(s) 266, 320, 694
NlaIII CATG 1 cut(s) 839
NlaIV GGNNCC 4 cut(s) 318, 435, 588, 691
NmuCI GTSAC 1 cut(s) 3
NsbI TGCGCA 1 cut(s) 384
NsiI ATGCAT 1 cut(s) 984
NspV TTCGAA 2 cut(s) 168, 204
PceI AGGCCT 1 cut(s) 520
PciSI GCTCTTC 1 cut(s) 47
PcsI WCGNNNNNNNCGW 3 cut(s) 186, 192, 210
PfeI GAWTC 3 cut(s) 595, 935, 1021
PflFI GACNNNGTC 2 cut(s) 179, 194
PfoI TCCNGGA 1 cut(s) 318
PkrI GCNGC 4 cut(s) 754, 757, 777, 796
PluTI GGCGCC 1 cut(s) 590
PpuMI RGGWCCY 1 cut(s) 689
Psp1406I AACGTT 2 cut(s) 256, 1038
Psp5II RGGWCCY 1 cut(s) 689
Psp6I CCWGG 4 cut(s) 275, 599, 667, 906
PspGI CCWGG 4 cut(s) 275, 599, 667, 906
PspN4I GGNNCC 4 cut(s) 318, 435, 588, 691
PspPI GGNCC 3 cut(s) 279, 316, 689
PspPPI RGGWCCY 1 cut(s) 689
PsyI GACNNNGTC 2 cut(s) 179, 194
PvuII CAGCTG 1 cut(s) 496
RsaI GTAC 2 cut(s) 339, 464
RsaNI GTAC 2 cut(s) 338, 463
SapI GCTCTTC 1 cut(s) 47
SaqAI TTAA 4 cut(s) 531, 647, 1017, 1071
SatI GCNGC 4 cut(s) 753, 756, 776, 795
Sau96I GGNCC 3 cut(s) 279, 316, 689
ScrFI CCNGG 7 cut(s) 266, 277, 320, 601, 669, 694, 908
SduI GDGCHC 1 cut(s) 438
SfaNI GCATC 3 cut(s) 714, 806, 969
SfoI GGCGCC 1 cut(s) 588
SfuI TTCGAA 2 cut(s) 168, 204
SinI GGWCC 2 cut(s) 316, 689
Sse9I AATT 9 cut(s) 110, 249, 297, 310, 445, 451, 891, 1014, 1073
SseBI AGGCCT 1 cut(s) 520
SsiI CCGC 1 cut(s) 795
SspDI GGCGCC 1 cut(s) 586
SspI AATATT 1 cut(s) 809
SspMI CTAG 2 cut(s) 732, 765
StuI AGGCCT 1 cut(s) 520
StyD4I CCNGG 7 cut(s) 264, 275, 318, 599, 667, 692, 906
TaaI ACNGT 3 cut(s) 349, 653, 877
TaiI ACGT 3 cut(s) 183, 259, 1041
TaqI TCGA 4 cut(s) 168, 204, 219, 404
TasI AATT 9 cut(s) 110, 249, 297, 310, 445, 451, 891, 1014, 1073
TatI WGTACW 1 cut(s) 462
TauI GCSGC 1 cut(s) 797
TfiI GAWTC 3 cut(s) 595, 935, 1021
Tru1I TTAA 4 cut(s) 531, 647, 1017, 1071
Tru9I TTAA 4 cut(s) 531, 647, 1017, 1071
TscAI CASTG 3 cut(s) 354, 510, 658
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 3 cut(s) 752, 755, 775
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 2 cut(s) 558, 852
TspGWI ACGGA 1 cut(s) 304
TspRI CASTG 3 cut(s) 354, 510, 658
Tth111I GACNNNGTC 2 cut(s) 179, 194
VpaK11BI GGWCC 2 cut(s) 316, 689
XapI RAATTY 5 cut(s) 110, 310, 445, 451, 1073
XspI CTAG 2 cut(s) 732, 765
Zsp2I ATGCAT 1 cut(s) 984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.