Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0000712.1

Orthogroup ID

OG0001128

Physical Location & Seq

Genomic Coordinates Forward (+)

115368 .. 120433

Gene Structure 5066 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0000712.1_g000024.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 990 bp

atgaggcttcagtcaccagttgacttaagtaggcacgatatgaaaggttgtggaattgatggtcgtaggtcagggaaatcgtggctgaagctgaggtggcgtctttgcagctttacggggcgtgacacgcttggcgaacccgaggccgatgaaggcgtgcttaccggtgccatcctcgatcctgaggatgaagtagcgccacgattcgagcacggtcttgacggaggtcgctggaatttggatcctccagtcagatctctcaccggtcctagcacgacgttttccgctctcggcgagcttctcgtcgctgctggctgcatcactgactttgatgagtaccgctcggagattcctgttcccaagtccatattagacatttgtgccctttggaaacttaaagatgatgagagaggatgtggaggcaaacaatcgaaaggtgaaagtgtatgtaggaattatacattcattgagaccagatggaagaagctcagagttggtgatcttgtcaaggtgcacaaggatgagtactttcttactgatctgcttttgctttcatcaagctatgaggatggcattttctatgtggaaactatgaaccttgatggagaaacaaatttaaaattgaaccattcactagaggcgacatcccatcttcatgatgaaaattccttggaaagatttagggtagagatcaagtttcttgaaatggatgctggcaacctgacgtacaaccatgtcgcagttacagtccacaataatttgcaacagtctcaccaaatgttgcgaacgagcttccgagctcttcacaccaaatgcggcggcgtagatgcagcaagcgtgctccttgaaggtgcgaatggaggcggggcggtcgacggtccagagcttcacggtgtcatcccacgaggaggtgaacaaccagtcgcggcgagtgggattgtagtcggcggagctgacctcacgcatgtgctcgtggagggagaggagtag

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

329

Amino Acids

36.13

Weight (kDa)

5.21

Isoelectric Point (pI)

41.08

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001128)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G66055 AT5G66055
fragaria_vesca	FvH4_1g10990 FvH4_1g10990
malus_domestica	MD15G1236900.v1.1
prunus_persica	Prupe.7G175600_v2.0.a1
pyrus_communis	pycom15g21070
rosa_chinensis	RchiOBHm_Chr1g0316921 RchiOBHm_Chr1g0316981 RchiOBHm_Chr2g0098251 RchiOBHm_Chr2g0145411 RchiOBHm_Chr4g0390291 RchiOBHm_Chr5g0073551
rosa_laevigata	RLG00000001656 RLG00000016779 RLG00000027817 RLG00000030637 RLG00000032089
rosa_multiflora	Rmu_co8173478.1_g000001 Rmu_sc0000104.1_g000031 Rmu_sc0000712.1_g000024 Rmu_sc0001298.1_g000058 Rmu_sc0002048.1_g000015 Rmu_sc0002398.1_g000019 Rmu_sc0004138.1_g000005 Rmu_sc0007472.1_g000006 Rmu_sc0009922.1_g000006
rosa_roxburghii	Rroxscaffold_2G00094620 Rroxscaffold_2G00144120
rosa_rugosa	Rorug02G0071600 Rorug02G0547800 Rorug04G0008500 Rorug04G0008600 Rorug04G0008600 Rorug04G0208000 Rorug05G0455100.1
rosa_samantha	Rh1AG024600 Rh1AG024700 Rh1CG022000 Rh1CG022900 Rh1DG021700 Rh2AG118600 Rh2BG121700 Rh2BG300700 Rh2BG456300 Rh2CG123300 Rh2CG279400 Rh2DG123300 Rh2DG466200 Rh4AG245100 Rh4BG023700 Rh4BG025500 Rh4BG250300 Rh4DG100600 Rh5DG257900 Rh6AG184700
rosa_wichuraiana	Rw1G001670 Rw2G009310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	167
AccBSI	CCGCTC	2 cut(s)	287, 342
AccI	GTMKAC	1 cut(s)	873
AccII	CGCG	1 cut(s)	926
AciI	CCGC	8 cut(s)	285, 340, 816, 819, 864, 869, 926, 948
AclWI	GGATC	3 cut(s)	173, 236, 249
AcsI	RAATTY	3 cut(s)	235, 613, 664
AcuI	CTGAAG	1 cut(s)	107
AcyI	GRCGYC	1 cut(s)	100
AfaI	GTAC	3 cut(s)	338, 527, 728
AfiI	CCNNNNNNNGG	1 cut(s)	908
AflII	CTTAAG	1 cut(s)	25
AgeI	ACCGGT	2 cut(s)	164, 263
AgsI	TTSAA	3 cut(s)	625, 704, 848
AleI	CACNNNNGTG	1 cut(s)	965
AluBI	AGCT	9 cut(s)	91, 111, 298, 487, 561, 792, 800, 886, 953
AluI	AGCT	9 cut(s)	91, 111, 298, 487, 561, 792, 800, 886, 953
Alw21I	GWGCWC	5 cut(s)	213, 516, 802, 843, 972
Alw26I	GTCTC	2 cut(s)	464, 774
Alw44I	GTGCAC	1 cut(s)	512
AlwI	GGATC	3 cut(s)	173, 236, 249
Ama87I	CYCGRG	1 cut(s)	140
AoxI	GGCC	1 cut(s)	144
ApaLI	GTGCAC	1 cut(s)	512
ApeKI	GCWGC	4 cut(s)	108, 308, 315, 830
ApoI	RAATTY	3 cut(s)	235, 613, 664
AsiGI	ACCGGT	2 cut(s)	164, 263
AspLEI	GCGC	1 cut(s)	199
AspS9I	GGNCC	2 cut(s)	266, 878
AsuHPI	GGTGA	6 cut(s)	6, 253, 449, 509, 764, 923
AvaI	CYCGRG	1 cut(s)	140
AvaII	GGWCC	2 cut(s)	266, 878
AxyI	CCTNAGG	1 cut(s)	183
BaeGI	GKGCMC	2 cut(s)	385, 516
BamHI	GGATCC	1 cut(s)	241
BanI	GGYRCC	1 cut(s)	167
BanII	GRGCYC	1 cut(s)	802
BauI	CACGAG	2 cut(s)	903, 971
Bbv12I	GWGCWC	5 cut(s)	213, 516, 802, 843, 972
BbvCI	CCTCAGC	1 cut(s)	92
BbvI	GCAGC	4 cut(s)	120, 295, 302, 842
BccI	CCATC	6 cut(s)	53, 179, 471, 563, 596, 657
BcoDI	GTCTC	2 cut(s)	464, 774
BfaI	CTAG	2 cut(s)	270, 635
BfoI	RGCGCY	1 cut(s)	200
BfrI	CTTAAG	1 cut(s)	25
BglII	AGATCT	1 cut(s)	254
BisI	GCNGC	7 cut(s)	109, 309, 316, 817, 820, 831, 927
BlsI	GCNGC	7 cut(s)	110, 310, 317, 818, 821, 832, 928
BmcAI	AGTACT	1 cut(s)	527
Bme18I	GGWCC	2 cut(s)	266, 878
BmeT110I	CYCGRG	1 cut(s)	140
BmgT120I	GGNCC	2 cut(s)	266, 878
BmiI	GGNNCC	2 cut(s)	169, 243
BmsI	GCATC	3 cut(s)	327, 700, 817
BoxI	GACNNNNGTC	1 cut(s)	225
BplI	GAGNNNNNCTC	4 cut(s)	326, 358, 942, 974
BpmI	CTGGAG	1 cut(s)	231
Bpu10I	CCTNAGC	1 cut(s)	92
BsaHI	GRCGYC	1 cut(s)	100
BsaI	GGTCTC	1 cut(s)	464
BsaJI	CCNNGG	2 cut(s)	141, 669
BsaWI	WCCGGW	2 cut(s)	164, 263
Bsc4I	CCNNNNNNNGG	1 cut(s)	908
Bse118I	RCCGGY	2 cut(s)	164, 263
Bse1I	ACTGG	3 cut(s)	17, 248, 920
Bse21I	CCTNAGG	1 cut(s)	183
BseDI	CCNNGG	2 cut(s)	141, 669
BseGI	GGATG	8 cut(s)	171, 193, 419, 526, 574, 644, 715, 897
BseLI	CCNNNNNNNGG	1 cut(s)	908
BseMII	CTCAG	3 cut(s)	83, 174, 502
BseNI	ACTGG	3 cut(s)	17, 248, 920
BseRI	GAGGAG	1 cut(s)	921
BseSI	GKGCMC	2 cut(s)	385, 516
BseXI	GCAGC	4 cut(s)	120, 295, 302, 842
Bsh1236I	CGCG	1 cut(s)	926
Bsh1285I	CGRYCG	1 cut(s)	873
BshFI	GGCC	1 cut(s)	146
BshNI	GGYRCC	1 cut(s)	167
BshTI	ACCGGT	2 cut(s)	164, 263
BsiEI	CGRYCG	1 cut(s)	873
BsiHKAI	GWGCWC	5 cut(s)	213, 516, 802, 843, 972
BsiHKCI	CYCGRG	1 cut(s)	140
BsiSI	CCGG	2 cut(s)	165, 264
BslI	CCNNNNNNNGG	1 cut(s)	908
BsmAI	GTCTC	2 cut(s)	464, 774
BsnI	GGCC	1 cut(s)	146
Bso31I	GGTCTC	1 cut(s)	464
BsoBI	CYCGRG	1 cut(s)	140
Bsp1286I	GDGCHC	6 cut(s)	213, 385, 516, 802, 843, 972
Bsp143I	GATC	6 cut(s)	178, 241, 254, 499, 538, 690
BspACI	CCGC	8 cut(s)	285, 340, 816, 819, 864, 869, 926, 948
BspANI	GGCC	1 cut(s)	146
BspCNI	CTCAG	3 cut(s)	84, 175, 501
BspFNI	CGCG	1 cut(s)	926
BspHI	TCATGA	1 cut(s)	655
BspLI	GGNNCC	2 cut(s)	169, 243
BspPI	GGATC	3 cut(s)	173, 236, 249
BspQI	GCTCTTC	1 cut(s)	807
BspT107I	GGYRCC	1 cut(s)	167
BspTI	CTTAAG	1 cut(s)	25
BspTNI	GGTCTC	1 cut(s)	464
BsrBI	CCGCTC	2 cut(s)	287, 342
BsrFI	RCCGGY	2 cut(s)	164, 263
BsrI	ACTGG	3 cut(s)	17, 248, 920
BssAI	RCCGGY	2 cut(s)	164, 263
BssECI	CCNNGG	2 cut(s)	141, 669
BssMI	GATC	6 cut(s)	178, 241, 254, 499, 538, 690
BssNI	GRCGYC	1 cut(s)	100
BssSI	CACGAG	2 cut(s)	903, 971
BssT1I	CCWWGG	1 cut(s)	669
Bst2BI	CACGAG	2 cut(s)	903, 971
Bst4CI	ACNGT	5 cut(s)	215, 748, 768, 878, 893
Bst6I	CTCTTC	1 cut(s)	807
BstACI	GRCGYC	1 cut(s)	100
BstAFI	CTTAAG	1 cut(s)	25
BstC8I	GCNNGC	6 cut(s)	158, 296, 313, 715, 835, 839
BstDEI	CTNAG	3 cut(s)	92, 183, 488
BstF5I	GGATG	8 cut(s)	171, 193, 419, 526, 574, 644, 715, 897
BstFNI	CGCG	1 cut(s)	926
BstH2I	RGCGCY	1 cut(s)	200
BstHHI	GCGC	1 cut(s)	199
BstKTI	GATC	6 cut(s)	181, 244, 257, 502, 541, 693
BstMAI	GTCTC	2 cut(s)	464, 774
BstMBI	GATC	6 cut(s)	178, 241, 254, 499, 538, 690
BstMCI	CGRYCG	1 cut(s)	873
BstMWI	GCNNNNNNNGC	2 cut(s)	97, 127
BstNSI	RCATGY	1 cut(s)	968
BstPAI	GACNNNNGTC	1 cut(s)	225
BstSLI	GKGCMC	2 cut(s)	385, 516
BstUI	CGCG	1 cut(s)	926
BstV1I	GCAGC	4 cut(s)	120, 295, 302, 842
BstX2I	RGATCY	2 cut(s)	241, 254
BstYI	RGATCY	2 cut(s)	241, 254
Bsu36I	CCTNAGG	1 cut(s)	183
BsuRI	GGCC	1 cut(s)	146
BtsCI	GGATG	8 cut(s)	171, 193, 419, 526, 574, 644, 715, 897
BtsIMutI	CAGTG	1 cut(s)	321
Cac8I	GCNNGC	6 cut(s)	158, 296, 313, 715, 835, 839
CciI	TCATGA	1 cut(s)	655
CfoI	GCGC	1 cut(s)	199
Cfr10I	RCCGGY	2 cut(s)	164, 263
Cfr13I	GGNCC	2 cut(s)	266, 878
CseI	GACGC	1 cut(s)	89
Csp6I	GTAC	3 cut(s)	337, 526, 727
CspAI	ACCGGT	2 cut(s)	164, 263
CviAII	CATG	3 cut(s)	656, 734, 965
CviQI	GTAC	3 cut(s)	337, 526, 727
DdeI	CTNAG	3 cut(s)	92, 183, 488
DpnI	GATC	6 cut(s)	180, 243, 256, 501, 540, 692
DpnII	GATC	6 cut(s)	178, 241, 254, 499, 538, 690
DraI	TTTAAA	1 cut(s)	618
Eam1104I	CTCTTC	1 cut(s)	807
EarI	CTCTTC	1 cut(s)	807
EciI	GGCGGA	1 cut(s)	963
Ecl136II	GAGCTC	1 cut(s)	800
Eco130I	CCWWGG	1 cut(s)	669
Eco24I	GRGCYC	1 cut(s)	802
Eco31I	GGTCTC	1 cut(s)	464
Eco47I	GGWCC	2 cut(s)	266, 878
Eco53kI	GAGCTC	1 cut(s)	800
Eco57I	CTGAAG	1 cut(s)	107
Eco81I	CCTNAGG	1 cut(s)	183
Eco88I	CYCGRG	1 cut(s)	140
EcoICRI	GAGCTC	1 cut(s)	800
EcoT14I	CCWWGG	1 cut(s)	669
EcoT38I	GRGCYC	1 cut(s)	802
ErhI	CCWWGG	1 cut(s)	669
FaeI	CATG	3 cut(s)	659, 737, 968
FalI	AAGNNNNNCTT	2 cut(s)	144, 176
FatI	CATG	3 cut(s)	655, 733, 964
FauI	CCCGC	1 cut(s)	857
FblI	GTMKAC	1 cut(s)	873
Fnu4HI	GCNGC	7 cut(s)	109, 309, 316, 817, 820, 831, 927
FokI	GGATG	8 cut(s)	158, 200, 426, 533, 581, 631, 722, 884
FriOI	GRGCYC	1 cut(s)	802
Fsp4HI	GCNGC	7 cut(s)	109, 309, 316, 817, 820, 831, 927
FspBI	CTAG	2 cut(s)	270, 635
GlaI	GCGC	1 cut(s)	198
GluI	GCNGC	7 cut(s)	109, 309, 316, 817, 820, 831, 927
GsuI	CTGGAG	1 cut(s)	231
HaeII	RGCGCY	1 cut(s)	200
HaeIII	GGCC	1 cut(s)	146
HapII	CCGG	2 cut(s)	165, 264
HgaI	GACGC	1 cut(s)	89
HhaI	GCGC	1 cut(s)	199
Hin1I	GRCGYC	1 cut(s)	100
Hin1II	CATG	3 cut(s)	659, 737, 968
Hin6I	GCGC	1 cut(s)	197
HinP1I	GCGC	1 cut(s)	197
HincII	GTYRAC	2 cut(s)	22, 874
HindII	GTYRAC	2 cut(s)	22, 874
HinfI	GANTC	2 cut(s)	204, 349
HpaII	CCGG	2 cut(s)	165, 264
HphI	GGTGA	6 cut(s)	6, 253, 449, 509, 764, 923
Hpy166II	GTNNAC	5 cut(s)	22, 514, 751, 874, 914
Hpy188I	TCNGA	4 cut(s)	254, 346, 491, 797
Hpy188III	TCNNGA	5 cut(s)	182, 218, 656, 701, 881
Hpy8I	GTNNAC	5 cut(s)	22, 514, 751, 874, 914
Hpy99I	CGWCG	3 cut(s)	280, 308, 878
HpyAV	CCTTC	2 cut(s)	146, 842
HpyCH4III	ACNGT	5 cut(s)	215, 748, 768, 878, 893
HpyCH4IV	ACGT	2 cut(s)	278, 725
HpyCH4V	TGCA	5 cut(s)	108, 318, 514, 763, 830
HpyF10VI	GCNNNNNNNGC	2 cut(s)	97, 127
HpyF3I	CTNAG	3 cut(s)	92, 183, 488
HpySE526I	ACGT	2 cut(s)	278, 725
Hsp92I	GRCGYC	1 cut(s)	100
Hsp92II	CATG	3 cut(s)	659, 737, 968
HspAI	GCGC	1 cut(s)	197
Kzo9I	GATC	6 cut(s)	178, 241, 254, 499, 538, 690
LguI	GCTCTTC	1 cut(s)	807
LmnI	GCTCC	2 cut(s)	846, 950
Lsp1109I	GCAGC	4 cut(s)	120, 295, 302, 842
LweI	GCATC	3 cut(s)	327, 700, 817
MaeI	CTAG	2 cut(s)	270, 635
MaeII	ACGT	2 cut(s)	278, 725
MaeIII	GTNAC	3 cut(s)	12, 122, 742
MalI	GATC	6 cut(s)	180, 243, 256, 501, 540, 692
MbiI	CCGCTC	2 cut(s)	287, 342
MboI	GATC	6 cut(s)	178, 241, 254, 499, 538, 690
MboII	GAAGA	3 cut(s)	493, 644, 794
MflI	RGATCY	2 cut(s)	241, 254
MhlI	GDGCHC	6 cut(s)	213, 385, 516, 802, 843, 972
MluCI	AATT	7 cut(s)	54, 235, 454, 613, 620, 664, 757
MseI	TTAA	3 cut(s)	26, 396, 617
MslI	CAYNNNNRTG	3 cut(s)	519, 654, 965
MspCI	CTTAAG	1 cut(s)	25
MspI	CCGG	2 cut(s)	165, 264
MvnI	CGCG	1 cut(s)	926
MwoI	GCNNNNNNNGC	2 cut(s)	97, 127
NdeII	GATC	6 cut(s)	178, 241, 254, 499, 538, 690
NlaIII	CATG	3 cut(s)	659, 737, 968
NlaIV	GGNNCC	2 cut(s)	169, 243
NmeAIII	GCCGAG	1 cut(s)	270
NmuCI	GTSAC	2 cut(s)	12, 122
NspI	RCATGY	1 cut(s)	968
OliI	CACNNNNGTG	1 cut(s)	965
PagI	TCATGA	1 cut(s)	655
PciSI	GCTCTTC	1 cut(s)	807
PfeI	GAWTC	2 cut(s)	204, 349
PinAI	ACCGGT	2 cut(s)	164, 263
PkrI	GCNGC	7 cut(s)	110, 310, 317, 818, 821, 832, 928
PshAI	GACNNNNGTC	1 cut(s)	225
Psp124BI	GAGCTC	1 cut(s)	802
PspN4I	GGNNCC	2 cut(s)	169, 243
PspPI	GGNCC	2 cut(s)	266, 878
PsuI	RGATCY	2 cut(s)	241, 254
RsaI	GTAC	3 cut(s)	338, 527, 728
RsaNI	GTAC	3 cut(s)	337, 526, 727
RseI	CAYNNNNRTG	3 cut(s)	519, 654, 965
SacI	GAGCTC	1 cut(s)	802
SalI	GTCGAC	1 cut(s)	872
SapI	GCTCTTC	1 cut(s)	807
SaqAI	TTAA	3 cut(s)	26, 396, 617
SatI	GCNGC	7 cut(s)	109, 309, 316, 817, 820, 831, 927
Sau3AI	GATC	6 cut(s)	178, 241, 254, 499, 538, 690
Sau96I	GGNCC	2 cut(s)	266, 878
ScaI	AGTACT	1 cut(s)	527
SduI	GDGCHC	6 cut(s)	213, 385, 516, 802, 843, 972
SfaNI	GCATC	3 cut(s)	327, 700, 817
SinI	GGWCC	2 cut(s)	266, 878
SmiMI	CAYNNNNRTG	3 cut(s)	519, 654, 965
SmlI	CTYRAG	1 cut(s)	25
SmoI	CTYRAG	1 cut(s)	25
Sse9I	AATT	7 cut(s)	54, 235, 454, 613, 620, 664, 757
SsiI	CCGC	8 cut(s)	285, 340, 816, 819, 864, 869, 926, 948
SspMI	CTAG	2 cut(s)	270, 635
SstI	GAGCTC	1 cut(s)	802
StyI	CCWWGG	1 cut(s)	669
TaaI	ACNGT	5 cut(s)	215, 748, 768, 878, 893
TaiI	ACGT	2 cut(s)	281, 728
TaqI	TCGA	4 cut(s)	177, 207, 431, 873
TasI	AATT	7 cut(s)	54, 235, 454, 613, 620, 664, 757
TatI	WGTACW	1 cut(s)	525
TauI	GCSGC	3 cut(s)	819, 822, 929
TfiI	GAWTC	2 cut(s)	204, 349
Tru1I	TTAA	3 cut(s)	26, 396, 617
Tru9I	TTAA	3 cut(s)	26, 396, 617
TscAI	CASTG	1 cut(s)	328
TseFI	GTSAC	2 cut(s)	12, 122
TseI	GCWGC	4 cut(s)	108, 308, 315, 830
Tsp45I	GTSAC	2 cut(s)	12, 122
TspDTI	ATGAA	8 cut(s)	56, 165, 204, 454, 543, 608, 644, 675
TspGWI	ACGGA	1 cut(s)	237
TspRI	CASTG	1 cut(s)	328
Vha464I	CTTAAG	1 cut(s)	25
VneI	GTGCAC	1 cut(s)	512
VpaK11BI	GGWCC	2 cut(s)	266, 878
XapI	RAATTY	3 cut(s)	235, 613, 664
XceI	RCATGY	1 cut(s)	968
XmiI	GTMKAC	1 cut(s)	873
XspI	CTAG	2 cut(s)	270, 635
ZrmI	AGTACT	1 cut(s)	527

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.