RchiOBHm_Chr2g0098251

Ankyrin repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001128
Physical Location & Seq
Reverse (-)
10658059 .. 10660709
2651 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47305

Sequence Viewer

Length: 1260 bp
ATGTCACTCTCTTCAGCTCTAAACCCCCAAACCTCCAAGCTCTTCTCTCTCCTCCCCACCCCATCTCTCTCTTTCACACCCCACAAGCTCTCGCCACCCACCACCCTGAAATTCTCATCCAAACTCCACACCCTCTCACCCTCCTTCAACTCCTCTCACTCCACCTTGGAAGAAGACCACGTCATCGGCGACTGCGTCGTCTTCGAAGAGGGCGTGTTCGACGACCCGTATCTTCAAAACGACACCGAAATCGAACACTTCAACCCGGAAAAGCCGAGGCCCAGAAAACGCGTTGAAATTGAGACCGAGAATTTGGTGCCGGAGAAATGGAAGGAGGTGCAGGCCACAGTGAACATAACAAAGAAAGAGAGGAGGAAGATTGCGCAGGAGTTGGAGTTCGGTTCGAGGGTCGAGAAGAACAAGAGGGGGTTGGAGCCCATAAGGAATTTGAATTTGGAGGAGTACAAGGCGTATAGAGAGGCCAAGTTGAACCAGCTGAAGCCACTGGTTTTGGATAGGCCTGGGAAGTTTAAGGAGGAGGATGAAGAGTGTGAAGGTGGTGATGGGTTTGTGAGTGACAGAGTGGCGCCTAAGAATCCCAGGTGGGCTGTTTATGGGAAGGGTTTGGAGGATGTTACTGAGTTTTTTAACAGTGGGCATTATGAGCCTGGTGTGAAGAAATCTGAAGGACCCCGGCCTTTGTTTACGAAAGAGGAAAAGCTGATGCTGAATAGGCGAAAACCTGATATAGCTGCTGCCACTTCTAGTAAATGGCTTCCCCTTCACACTCTTGCCGCATCAGGAGAATATTACCTTATGGACGCTTTATTGAAACATGAAGCTGATATCAATGCTGTGGATAAGGATGATTGGACTGTTCTTCACAAAGCAATTATTGGTAAAAACCAGGCCATTACAAACTATCTTCTCAGAGAATCGGCTAATCCATTTGTTCGTGATAAAGATGGCGCTACCTTGATGCATTATGCTGTCCGGACAGCTTCAAGTCAAGCAATTAAGATTCTTCTGTTATACAACGTTGATATAAACCTTCAGGACAATGATGGGTGGACGCCCTTACATCTAGCTGTTCAAGGCCGAAGAACAGATGTAGTGAGGCTTTTGTTGATTAAAGGCGCGGATAAGACATTGAAAAACAAGGATGGATTGACCCCTCTTGAGCTTTGCCTCTATTCTGGCCGAGACACGAGGACTTACGAGCTGATTAAGCTATTGAAGCTTCTTCCCAAAACCCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 RRM_1

Protein Analysis

419

Amino Acids

47.65

Weight (kDa)

8.17

Isoelectric Point (pI)

42.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 253 - 319 1.5e-07 Ankyrin repeats (3 copies)
Ank_4 PF13637 261 - 310 2.4e-07 Ankyrin repeats (many copies)
Ank_5 PF13857 276 - 330 4.2e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 304 - 386 5.6e-17 Ankyrin repeats (3 copies)
Ank_5 PF13857 312 - 363 2.4e-10 Ankyrin repeats (many copies)
Ank_2 PF12796 327 - 401 3.3e-14 Ankyrin repeats (3 copies)
Ank_4 PF13637 338 - 376 1.8e-07 Ankyrin repeats (many copies)
Ank PF00023 355 - 387 4e-08 Ankyrin repeat
Ank_5 PF13857 358 - 396 7.3e-07 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 197
Acc16I TGCGCA 1 cut(s) 384
AccB1I GGYRCC 2 cut(s) 316, 586
AccII CGCG 2 cut(s) 291, 1139
AccIII TCCGGA 1 cut(s) 993
AciI CCGC 2 cut(s) 795, 1139
AclI AACGTT 1 cut(s) 1038
AcoI YGGCCR 1 cut(s) 1198
AcsI RAATTY 4 cut(s) 110, 310, 445, 451
AcuI CTGAAG 3 cut(s) 518, 705, 1037
AcyI GRCGYC 2 cut(s) 587, 1073
AfaI GTAC 1 cut(s) 464
AflIII ACRYGT 1 cut(s) 289
AjiI CACGTC 1 cut(s) 181
AjnI CCWGG 4 cut(s) 520, 599, 667, 906
AjuI GAANNNNNNNTTGG 2 cut(s) 437, 469
AloI GAACNNNNNNTCC 2 cut(s) 380, 412
Alw26I GTCTC 2 cut(s) 296, 1197
Aor13HI TCCGGA 1 cut(s) 993
AoxI GGCC 8 cut(s) 278, 342, 480, 518, 695, 909, 1096, 1198
ApeKI GCWGC 2 cut(s) 752, 755
ApoI RAATTY 4 cut(s) 110, 310, 445, 451
ArsI GACNNNNNNTTYG 2 cut(s) 295, 327
AspLEI GCGC 4 cut(s) 385, 589, 971, 1139
AspS9I GGNCC 2 cut(s) 279, 689
AsuC2I CCSGG 2 cut(s) 266, 694
AsuHPI GGTGA 2 cut(s) 129, 572
AsuII TTCGAA 1 cut(s) 204
AvaII GGWCC 1 cut(s) 689
BanI GGYRCC 2 cut(s) 316, 586
BanII GRGCYC 1 cut(s) 438
BauI CACGAG 1 cut(s) 1207
BbsI GAAGAC 2 cut(s) 180, 193
BbvI GCAGC 2 cut(s) 739, 742
BccI CCATC 5 cut(s) 70, 557, 959, 1058, 1157
BciT130I CCWGG 4 cut(s) 522, 601, 669, 908
BcnI CCSGG 2 cut(s) 266, 694
BcoDI GTCTC 2 cut(s) 296, 1197
BfaI CTAG 2 cut(s) 765, 1085
BfoI RGCGCY 2 cut(s) 590, 972
BisI GCNGC 3 cut(s) 753, 756, 795
BlsI GCNGC 3 cut(s) 754, 757, 796
Bme1390I CCNGG 6 cut(s) 266, 522, 601, 669, 694, 908
Bme18I GGWCC 1 cut(s) 689
BmgBI CACGTC 1 cut(s) 181
BmgT120I GGNCC 2 cut(s) 279, 689
BmiI GGNNCC 4 cut(s) 318, 435, 588, 691
BmrFI CCNGG 6 cut(s) 266, 522, 601, 669, 694, 908
BmsI GCATC 3 cut(s) 714, 806, 969
BpiI GAAGAC 2 cut(s) 180, 193
Bpu14I TTCGAA 1 cut(s) 204
BpuEI CTTGAG 1 cut(s) 1199
BpuMI CCSGG 2 cut(s) 266, 694
BsaHI GRCGYC 2 cut(s) 587, 1073
BsaI GGTCTC 1 cut(s) 296
BsaJI CCNNGG 5 cut(s) 165, 275, 521, 599, 692
BsaWI WCCGGW 1 cut(s) 993
BsaXI ACNNNNNCTCC 2 cut(s) 380, 410
Bse1I ACTGG 1 cut(s) 510
BseAI TCCGGA 1 cut(s) 993
BseBI CCWGG 4 cut(s) 522, 601, 669, 908
BseDI CCNNGG 5 cut(s) 165, 275, 521, 599, 692
BseGI GGATG 5 cut(s) 116, 547, 637, 871, 1168
BseMII CTCAG 2 cut(s) 630, 943
BseNI ACTGG 1 cut(s) 510
BseRI GAGGAG 5 cut(s) 41, 142, 385, 473, 551
BseXI GCAGC 2 cut(s) 739, 742
BsgI GTGCAG 1 cut(s) 359
Bsh1236I CGCG 2 cut(s) 291, 1139
BshFI GGCC 8 cut(s) 280, 344, 482, 520, 697, 911, 1098, 1200
BshNI GGYRCC 2 cut(s) 316, 586
BsiSI CCGG 4 cut(s) 266, 320, 694, 994
BsmAI GTCTC 2 cut(s) 296, 1197
BsnI GGCC 8 cut(s) 280, 344, 482, 520, 697, 911, 1098, 1200
Bso31I GGTCTC 1 cut(s) 296
Bsp119I TTCGAA 1 cut(s) 204
Bsp1286I GDGCHC 1 cut(s) 438
Bsp13I TCCGGA 1 cut(s) 993
BspACI CCGC 2 cut(s) 795, 1139
BspANI GGCC 8 cut(s) 280, 344, 482, 520, 697, 911, 1098, 1200
BspCNI CTCAG 2 cut(s) 631, 942
BspEI TCCGGA 1 cut(s) 993
BspFNI CGCG 2 cut(s) 291, 1139
BspLI GGNNCC 4 cut(s) 318, 435, 588, 691
BspQI GCTCTTC 1 cut(s) 47
BspT104I TTCGAA 1 cut(s) 204
BspT107I GGYRCC 2 cut(s) 316, 586
BspTNI GGTCTC 1 cut(s) 296
BsrI ACTGG 1 cut(s) 510
BssECI CCNNGG 5 cut(s) 165, 275, 521, 599, 692
BssNI GRCGYC 2 cut(s) 587, 1073
BssSI CACGAG 1 cut(s) 1207
BssT1I CCWWGG 1 cut(s) 165
Bst2BI CACGAG 1 cut(s) 1207
Bst2UI CCWGG 4 cut(s) 522, 601, 669, 908
Bst4CI ACNGT 3 cut(s) 349, 653, 877
Bst6I CTCTTC 4 cut(s) 16, 47, 201, 540
BstACI GRCGYC 2 cut(s) 587, 1073
BstBI TTCGAA 1 cut(s) 204
BstC8I GCNNGC 1 cut(s) 342
BstDEI CTNAG 3 cut(s) 591, 639, 929
BstF5I GGATG 5 cut(s) 116, 547, 637, 871, 1168
BstFNI CGCG 2 cut(s) 291, 1139
BstH2I RGCGCY 2 cut(s) 590, 972
BstHHI GCGC 4 cut(s) 385, 589, 971, 1139
BstMAI GTCTC 2 cut(s) 296, 1197
BstMWI GCNNNNNNNGC 4 cut(s) 664, 733, 1228, 1237
BstNI CCWGG 4 cut(s) 522, 601, 669, 908
BstSCI CCNGG 6 cut(s) 264, 520, 599, 667, 692, 906
BstUI CGCG 2 cut(s) 291, 1139
BstV1I GCAGC 2 cut(s) 739, 742
BstV2I GAAGAC 2 cut(s) 180, 193
BsuRI GGCC 8 cut(s) 280, 344, 482, 520, 697, 911, 1098, 1200
BtrI CACGTC 1 cut(s) 181
BtsCI GGATG 5 cut(s) 116, 547, 637, 871, 1168
BtsIMutI CAGTG 3 cut(s) 354, 503, 658
Cac8I GCNNGC 1 cut(s) 342
CfoI GCGC 4 cut(s) 385, 589, 971, 1139
Cfr13I GGNCC 2 cut(s) 279, 689
CseI GACGC 3 cut(s) 184, 830, 1081
Csp6I GTAC 1 cut(s) 463
CspCI CAANNNNNGTGG 2 cut(s) 492, 527
CviAII CATG 1 cut(s) 836
CviQI GTAC 1 cut(s) 463
DdeI CTNAG 3 cut(s) 591, 639, 929
DinI GGCGCC 1 cut(s) 588
DrdI GACNNNNNNGTC 1 cut(s) 197
DseDI GACNNNNNNGTC 1 cut(s) 197
EaeI YGGCCR 1 cut(s) 1198
Eam1104I CTCTTC 4 cut(s) 16, 47, 201, 540
EarI CTCTTC 4 cut(s) 16, 47, 201, 540
Eco130I CCWWGG 1 cut(s) 165
Eco147I AGGCCT 1 cut(s) 520
Eco24I GRGCYC 1 cut(s) 438
Eco31I GGTCTC 1 cut(s) 296
Eco32I GATATC 1 cut(s) 847
Eco47I GGWCC 1 cut(s) 689
Eco57I CTGAAG 3 cut(s) 518, 705, 1037
EcoO109I RGGNCCY 1 cut(s) 689
EcoRII CCWGG 4 cut(s) 520, 599, 667, 906
EcoRV GATATC 1 cut(s) 847
EcoT14I CCWWGG 1 cut(s) 165
EcoT22I ATGCAT 1 cut(s) 984
EcoT38I GRGCYC 1 cut(s) 438
EgeI GGCGCC 1 cut(s) 588
EheI GGCGCC 1 cut(s) 588
ErhI CCWWGG 1 cut(s) 165
FaeI CATG 1 cut(s) 839
FatI CATG 1 cut(s) 835
Fnu4HI GCNGC 3 cut(s) 753, 756, 795
FokI GGATG 5 cut(s) 103, 554, 644, 878, 1175
FriOI GRGCYC 1 cut(s) 438
Fsp4HI GCNGC 3 cut(s) 753, 756, 795
FspBI CTAG 2 cut(s) 765, 1085
FspI TGCGCA 1 cut(s) 384
GlaI GCGC 4 cut(s) 384, 588, 970, 1138
GluI GCNGC 3 cut(s) 753, 756, 795
HaeII RGCGCY 2 cut(s) 590, 972
HaeIII GGCC 8 cut(s) 280, 344, 482, 520, 697, 911, 1098, 1200
HapII CCGG 4 cut(s) 266, 320, 694, 994
HgaI GACGC 3 cut(s) 184, 830, 1081
HhaI GCGC 4 cut(s) 385, 589, 971, 1139
Hin1I GRCGYC 2 cut(s) 587, 1073
Hin1II CATG 1 cut(s) 839
Hin6I GCGC 4 cut(s) 383, 587, 969, 1137
HinP1I GCGC 4 cut(s) 383, 587, 969, 1137
HindIII AAGCTT 1 cut(s) 1238
HinfI GANTC 3 cut(s) 595, 935, 1021
HpaII CCGG 4 cut(s) 266, 320, 694, 994
HphI GGTGA 2 cut(s) 129, 572
Hpy166II GTNNAC 3 cut(s) 352, 705, 1071
Hpy188I TCNGA 2 cut(s) 685, 932
Hpy188III TCNNGA 6 cut(s) 412, 801, 956, 994, 1055, 1178
Hpy8I GTNNAC 3 cut(s) 352, 705, 1071
Hpy99I CGWCG 2 cut(s) 200, 224
HpyAV CCTTC 7 cut(s) 154, 325, 548, 613, 680, 791, 1061
HpyCH4III ACNGT 3 cut(s) 349, 653, 877
HpyCH4IV ACGT 2 cut(s) 180, 1038
HpyCH4V TGCA 2 cut(s) 340, 982
HpyF10VI GCNNNNNNNGC 4 cut(s) 664, 733, 1228, 1237
HpyF3I CTNAG 3 cut(s) 591, 639, 929
HpySE526I ACGT 2 cut(s) 180, 1038
Hsp92I GRCGYC 2 cut(s) 587, 1073
Hsp92II CATG 1 cut(s) 839
HspAI GCGC 4 cut(s) 383, 587, 969, 1137
KasI GGCGCC 1 cut(s) 586
Kpn2I TCCGGA 1 cut(s) 993
LguI GCTCTTC 1 cut(s) 47
LmnI GCTCC 1 cut(s) 433
Lsp1109I GCAGC 2 cut(s) 739, 742
LweI GCATC 3 cut(s) 714, 806, 969
MaeI CTAG 2 cut(s) 765, 1085
MaeII ACGT 2 cut(s) 180, 1038
MaeIII GTNAC 3 cut(s) 3, 575, 634
MhlI GDGCHC 1 cut(s) 438
MluCI AATT 7 cut(s) 110, 297, 310, 445, 451, 891, 1014
MluI ACGCGT 1 cut(s) 289
Mly113I GGCGCC 1 cut(s) 587
MmeI TCCRAC 2 cut(s) 372, 411
Mph1103I ATGCAT 1 cut(s) 984
MroI TCCGGA 1 cut(s) 993
MseI TTAA 6 cut(s) 531, 648, 1017, 1131, 1227, 1258
MspA1I CMGCKG 1 cut(s) 496
MspI CCGG 4 cut(s) 266, 320, 694, 994
MspR9I CCNGG 6 cut(s) 266, 522, 601, 669, 694, 908
MvaI CCWGG 4 cut(s) 522, 601, 669, 908
MvnI CGCG 2 cut(s) 291, 1139
MwoI GCNNNNNNNGC 4 cut(s) 664, 733, 1228, 1237
NarI GGCGCC 1 cut(s) 587
NciI CCSGG 2 cut(s) 266, 694
NlaIII CATG 1 cut(s) 839
NlaIV GGNNCC 4 cut(s) 318, 435, 588, 691
NmeAIII GCCGAG 2 cut(s) 300, 1226
NmuCI GTSAC 2 cut(s) 3, 575
NsbI TGCGCA 1 cut(s) 384
NsiI ATGCAT 1 cut(s) 984
NspV TTCGAA 1 cut(s) 204
PceI AGGCCT 1 cut(s) 520
PciSI GCTCTTC 1 cut(s) 47
PcsI WCGNNNNNNNCGW 3 cut(s) 186, 192, 210
PfeI GAWTC 3 cut(s) 595, 935, 1021
PflFI GACNNNGTC 2 cut(s) 179, 194
PkrI GCNGC 3 cut(s) 754, 757, 796
PluTI GGCGCC 1 cut(s) 590
PpuMI RGGWCCY 1 cut(s) 689
Psp1406I AACGTT 1 cut(s) 1038
Psp5II RGGWCCY 1 cut(s) 689
Psp6I CCWGG 4 cut(s) 520, 599, 667, 906
PspGI CCWGG 4 cut(s) 520, 599, 667, 906
PspN4I GGNNCC 4 cut(s) 318, 435, 588, 691
PspPI GGNCC 2 cut(s) 279, 689
PspPPI RGGWCCY 1 cut(s) 689
PsyI GACNNNGTC 2 cut(s) 179, 194
PvuII CAGCTG 1 cut(s) 496
RsaI GTAC 1 cut(s) 464
RsaNI GTAC 1 cut(s) 463
SapI GCTCTTC 1 cut(s) 47
SaqAI TTAA 6 cut(s) 531, 648, 1017, 1131, 1227, 1258
SatI GCNGC 3 cut(s) 753, 756, 795
Sau96I GGNCC 2 cut(s) 279, 689
ScrFI CCNGG 6 cut(s) 266, 522, 601, 669, 694, 908
SduI GDGCHC 1 cut(s) 438
SfaNI GCATC 3 cut(s) 714, 806, 969
SfoI GGCGCC 1 cut(s) 588
SfuI TTCGAA 1 cut(s) 204
SinI GGWCC 1 cut(s) 689
SmlI CTYRAG 1 cut(s) 1178
SmoI CTYRAG 1 cut(s) 1178
Sse9I AATT 7 cut(s) 110, 297, 310, 445, 451, 891, 1014
SseBI AGGCCT 1 cut(s) 520
SsiI CCGC 2 cut(s) 795, 1139
SspDI GGCGCC 1 cut(s) 586
SspI AATATT 1 cut(s) 809
SspMI CTAG 2 cut(s) 765, 1085
StuI AGGCCT 1 cut(s) 520
StyD4I CCNGG 6 cut(s) 264, 520, 599, 667, 692, 906
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 3 cut(s) 349, 653, 877
TaiI ACGT 2 cut(s) 183, 1041
TaqI TCGA 5 cut(s) 204, 219, 252, 404, 411
TaqII GACCGA 1 cut(s) 320
TasI AATT 7 cut(s) 110, 297, 310, 445, 451, 891, 1014
TatI WGTACW 1 cut(s) 462
TauI GCSGC 1 cut(s) 797
TfiI GAWTC 3 cut(s) 595, 935, 1021
Tru1I TTAA 6 cut(s) 531, 648, 1017, 1131, 1227, 1258
Tru9I TTAA 6 cut(s) 531, 648, 1017, 1131, 1227, 1258
TscAI CASTG 3 cut(s) 354, 510, 658
TseFI GTSAC 2 cut(s) 3, 575
TseI GCWGC 2 cut(s) 752, 755
Tsp45I GTSAC 2 cut(s) 3, 575
TspDTI ATGAA 2 cut(s) 558, 852
TspRI CASTG 3 cut(s) 354, 510, 658
Tth111I GACNNNGTC 2 cut(s) 179, 194
VpaK11BI GGWCC 1 cut(s) 689
XapI RAATTY 4 cut(s) 110, 310, 445, 451
XspI CTAG 2 cut(s) 765, 1085
Zsp2I ATGCAT 1 cut(s) 984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.