FvH4_1g07650

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000515
Physical Location & Seq
Reverse (-)
4066510 .. 4068697
2188 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g07650.t2

Sequence Viewer

Length: 810 bp
ATGGCAGAGGCAGCCCAGAACCGAGTGAGTCAGCCAGAGAAAATAGTTATAGCCAACAAACATGGTGAAAAGCTTGTGGGATTATTGCATGAAACCGGTTCTAAAGAGCTTGTGATCTTGTGCCATGGTGCTCGATCCCACAAGGGAGACAATATTATGACTAAACTTGCTCTTGCTTTGGAAAAGGAAGGAATTAGTGCTTTTCGTTTCGATTTTGCTGGAAATGGAGAAAGTGAAGGTACCTTTCAGTTTGGTCACTACTGGAGAGAGGTTGATGATTTACGTGCTGTAATCCAACACTTCTCTAGGACAAACCATACAGTAAGCGCAATTATTGGCCACAGTAAAGGAGGGGATGAAGTGCTTCTGTATGCTTCCAAGTATGGTGATGTTCGCACAGTTGTCAATGTTTCTGGGCGTTATGATCTGAATGGAGGCCTTGAGCTACGTTTGGGGAAAAACTTTATGGAAAGGATCAAGAAGCAAGAGTATTTGGATATCAAGGATAAGACAGGAAGTTTTGATTGTCGTGTGACGGAGGAAAGTTTGCTCGATCGCATGAATACTAATATGCATGAATCATGCCTTAAGATTGCCAAAGATTGCCGGGTGTTGACAGTCCATGGATCTGAAGATGACGTGATCCCTGTTGGAGATGCACTGGAGTTTGCCAAGATAATACCTAACCACAAGTTACAAGTTATACAAGGAGCTGATCATTATTACAGCGATCACCAAGCCAAGTTAGCATCAAGTATTTTGGACTTCCTCAAGGCAGCTATCCAAGAGGGCAAGATTACTTCCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

270

Amino Acids

29.92

Weight (kDa)

6.35

Isoelectric Point (pI)

28.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 3 - 188 1.4e-10 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47590 AT3G47590
fragaria_vesca FvH4_1g07650 FvH4_1g07670 FvH4_1g07671 FvH4_1g07680 FvH4_1g07690 FvH4_1g07700 FvH4_1g07700
malus_domestica MD02G1080900.v1.1 MD02G1081000.v1.1 MD02G1081300.v1.1 MD02G1081400.v1.1 MD02G1094000.v1.1 MD15G1208700.v1.1
prunus_persica Prupe.7G208200_v2.0.a1 Prupe.7G208200_v2.0.a1
pyrus_communis pycom02g07440 pycom15g18480
rosa_chinensis RchiOBHm_Chr2g0093511 RchiOBHm_Chr2g0093521 RchiOBHm_Chr2g0093531 RchiOBHm_Chr2g0093541 RchiOBHm_Chr2g0093551 RchiOBHm_Chr2g0093561
rosa_laevigata RLG00000016413 RLG00000016414 RLG00000016415 RLG00000016416 RLG00000016417
rosa_multiflora Rmu_sc0004423.1_g000003 Rmu_sc0004423.1_g000004 Rmu_sc0004423.1_g000005 Rmu_sc0004423.1_g000006 Rmu_sc0006964.1_g000003 Rmu_sc0039672.1_g000001 Rmu_sc0039672.1_g000003
rosa_roxburghii Rroxscaffold_2G00147990 Rroxscaffold_2G00148000 Rroxscaffold_2G00148010 Rroxscaffold_2G00148020 Rroxscaffold_4G00313540
rosa_rugosa Rorug01G0140600.1 Rorug01G0140700.1 Rorug02G0037000 Rorug02G0037100 Rorug02G0037200 Rorug02G0037300 Rorug02G0037400 Rorug02G0037500 Rorug02G0037600
rosa_samantha Rh2AG083400 Rh2AG083500 Rh2AG083700 Rh2AG083800 Rh2AG083900 Rh2AG084000 Rh2BG084200 Rh2BG084300 Rh2BG084400 Rh2BG084500 Rh2BG084600 Rh2CG086300 Rh2CG086400 Rh2CG086500 Rh2CG086700 Rh2CG086800 Rh2CG086900 Rh2DG082200 Rh2DG082300 Rh2DG082400 Rh2DG082500 Rh2DG082600 Rh2DG082700
rosa_wichuraiana Rw1G013200 Rw2G006360 Rw2G006370 Rw2G006380 Rw2G006390 Rw2G006400 Rw2G007130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 239
AccB1I GGYRCC 1 cut(s) 239
AclWI GGATC 4 cut(s) 129, 482, 634, 637
AcoI YGGCCR 1 cut(s) 337
AcuI CTGAAG 1 cut(s) 651
AfaI GTAC 1 cut(s) 241
AflII CTTAAG 1 cut(s) 587
AgeI ACCGGT 1 cut(s) 95
AjiI CACGTC 1 cut(s) 640
AluBI AGCT 5 cut(s) 73, 109, 445, 713, 779
AluI AGCT 5 cut(s) 73, 109, 445, 713, 779
Alw21I GWGCWC 1 cut(s) 133
Alw26I GTCTC 1 cut(s) 141
AlwI GGATC 4 cut(s) 129, 482, 634, 637
AoxI GGCC 2 cut(s) 337, 436
ApeKI GCWGC 2 cut(s) 11, 776
AsiGI ACCGGT 1 cut(s) 95
Asp700I GAANNNNTTC 1 cut(s) 363
Asp718I GGTACC 1 cut(s) 239
AspLEI GCGC 1 cut(s) 329
AsuC2I CCSGG 1 cut(s) 608
AsuHPI GGTGA 3 cut(s) 77, 398, 725
BalI TGGCCA 1 cut(s) 339
BanI GGYRCC 1 cut(s) 239
Bbv12I GWGCWC 1 cut(s) 133
BbvI GCAGC 2 cut(s) 23, 788
BclI TGATCA 1 cut(s) 715
BcnI CCSGG 1 cut(s) 608
BcoDI GTCTC 1 cut(s) 141
BfaI CTAG 2 cut(s) 306, 808
BfrI CTTAAG 1 cut(s) 587
BisI GCNGC 2 cut(s) 12, 777
BlsI GCNGC 2 cut(s) 13, 778
Bme1390I CCNGG 1 cut(s) 608
BmgBI CACGTC 1 cut(s) 640
BmiI GGNNCC 1 cut(s) 241
BmrFI CCNGG 1 cut(s) 608
BmsI GCATC 2 cut(s) 646, 758
BpmI CTGGAG 2 cut(s) 283, 683
BpuEI CTTGAG 2 cut(s) 461, 755
BpuMI CCSGG 1 cut(s) 608
BsaAI YACGTR 1 cut(s) 284
BsaJI CCNNGG 2 cut(s) 124, 622
BsaWI WCCGGW 1 cut(s) 95
Bse118I RCCGGY 1 cut(s) 95
Bse1I ACTGG 2 cut(s) 266, 666
BseDI CCNNGG 2 cut(s) 124, 622
BseGI GGATG 1 cut(s) 361
BseNI ACTGG 2 cut(s) 266, 666
BseXI GCAGC 2 cut(s) 23, 788
Bsh1285I CGRYCG 1 cut(s) 556
BshFI GGCC 2 cut(s) 339, 438
BshNI GGYRCC 1 cut(s) 239
BshTI ACCGGT 1 cut(s) 95
BsiEI CGRYCG 1 cut(s) 556
BsiHKAI GWGCWC 1 cut(s) 133
BsiSI CCGG 2 cut(s) 96, 607
BsmAI GTCTC 1 cut(s) 141
BsnI GGCC 2 cut(s) 339, 438
Bsp1286I GDGCHC 1 cut(s) 133
Bsp143I GATC 9 cut(s) 114, 134, 424, 474, 553, 626, 642, 715, 730
Bsp19I CCATGG 2 cut(s) 124, 622
BspANI GGCC 2 cut(s) 339, 438
BspLI GGNNCC 1 cut(s) 241
BspPI GGATC 4 cut(s) 129, 482, 634, 637
BspT107I GGYRCC 1 cut(s) 239
BspTI CTTAAG 1 cut(s) 587
BsrFI RCCGGY 1 cut(s) 95
BsrI ACTGG 2 cut(s) 266, 666
BssAI RCCGGY 1 cut(s) 95
BssECI CCNNGG 2 cut(s) 124, 622
BssMI GATC 9 cut(s) 114, 134, 424, 474, 553, 626, 642, 715, 730
BssT1I CCWWGG 2 cut(s) 124, 622
Bst4CI ACNGT 4 cut(s) 322, 344, 400, 619
BstAFI CTTAAG 1 cut(s) 587
BstBAI YACGTR 1 cut(s) 284
BstDSI CCRYGG 2 cut(s) 124, 622
BstF5I GGATG 1 cut(s) 361
BstHHI GCGC 1 cut(s) 329
BstKTI GATC 9 cut(s) 117, 137, 427, 477, 556, 629, 645, 718, 733
BstMAI GTCTC 1 cut(s) 141
BstMBI GATC 9 cut(s) 114, 134, 424, 474, 553, 626, 642, 715, 730
BstMCI CGRYCG 1 cut(s) 556
BstMWI GCNNNNNNNGC 2 cut(s) 11, 746
BstSCI CCNGG 1 cut(s) 606
BstV1I GCAGC 2 cut(s) 23, 788
BstX2I RGATCY 1 cut(s) 626
BstYI RGATCY 1 cut(s) 626
BsuRI GGCC 2 cut(s) 339, 438
BtgI CCRYGG 2 cut(s) 124, 622
BtrI CACGTC 1 cut(s) 640
BtsCI GGATG 1 cut(s) 361
BtsIMutI CAGTG 1 cut(s) 659
CfoI GCGC 1 cut(s) 329
Cfr10I RCCGGY 1 cut(s) 95
Csp6I GTAC 1 cut(s) 240
CspAI ACCGGT 1 cut(s) 95
CviAII CATG 7 cut(s) 62, 89, 125, 559, 575, 582, 623
CviQI GTAC 1 cut(s) 240
DpnI GATC 9 cut(s) 116, 136, 426, 476, 555, 628, 644, 717, 732
DpnII GATC 9 cut(s) 114, 134, 424, 474, 553, 626, 642, 715, 730
EaeI YGGCCR 1 cut(s) 337
Eco130I CCWWGG 2 cut(s) 124, 622
Eco147I AGGCCT 1 cut(s) 438
Eco32I GATATC 1 cut(s) 499
Eco57I CTGAAG 1 cut(s) 651
EcoRV GATATC 1 cut(s) 499
EcoT14I CCWWGG 2 cut(s) 124, 622
EcoT22I ATGCAT 1 cut(s) 576
ErhI CCWWGG 2 cut(s) 124, 622
FaeI CATG 7 cut(s) 65, 92, 128, 562, 578, 585, 626
FatI CATG 7 cut(s) 61, 88, 124, 558, 574, 581, 622
FbaI TGATCA 1 cut(s) 715
Fnu4HI GCNGC 2 cut(s) 12, 777
FokI GGATG 1 cut(s) 368
Fsp4HI GCNGC 2 cut(s) 12, 777
FspBI CTAG 2 cut(s) 306, 808
GlaI GCGC 1 cut(s) 328
GluI GCNGC 2 cut(s) 12, 777
GsuI CTGGAG 2 cut(s) 283, 683
HaeIII GGCC 2 cut(s) 339, 438
HapII CCGG 2 cut(s) 96, 607
HhaI GCGC 1 cut(s) 329
Hin1II CATG 7 cut(s) 65, 92, 128, 562, 578, 585, 626
Hin6I GCGC 1 cut(s) 327
HinP1I GCGC 1 cut(s) 327
HincII GTYRAC 1 cut(s) 615
HindII GTYRAC 1 cut(s) 615
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 2 cut(s) 28, 578
HpaII CCGG 2 cut(s) 96, 607
HphI GGTGA 3 cut(s) 77, 398, 725
Hpy166II GTNNAC 1 cut(s) 615
Hpy188I TCNGA 2 cut(s) 429, 631
Hpy188III TCNNGA 1 cut(s) 478
Hpy8I GTNNAC 1 cut(s) 615
HpyAV CCTTC 2 cut(s) 182, 230
HpyCH4III ACNGT 4 cut(s) 322, 344, 400, 619
HpyCH4IV ACGT 3 cut(s) 283, 448, 639
HpyCH4V TGCA 3 cut(s) 88, 574, 659
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 746
HpySE526I ACGT 3 cut(s) 283, 448, 639
Hsp92II CATG 7 cut(s) 65, 92, 128, 562, 578, 585, 626
HspAI GCGC 1 cut(s) 327
KpnI GGTACC 1 cut(s) 243
Ksp22I TGATCA 1 cut(s) 715
Kzo9I GATC 9 cut(s) 114, 134, 424, 474, 553, 626, 642, 715, 730
LmnI GCTCC 1 cut(s) 710
Lsp1109I GCAGC 2 cut(s) 23, 788
LweI GCATC 2 cut(s) 646, 758
MaeI CTAG 2 cut(s) 306, 808
MaeII ACGT 3 cut(s) 283, 448, 639
MaeIII GTNAC 3 cut(s) 254, 532, 693
MalI GATC 9 cut(s) 116, 136, 426, 476, 555, 628, 644, 717, 732
MboI GATC 9 cut(s) 114, 134, 424, 474, 553, 626, 642, 715, 730
MboII GAAGA 1 cut(s) 644
MflI RGATCY 1 cut(s) 626
MhlI GDGCHC 1 cut(s) 133
MlsI TGGCCA 1 cut(s) 339
MluCI AATT 2 cut(s) 192, 330
MluNI TGGCCA 1 cut(s) 339
MlyI GAGTC 1 cut(s) 37
MmeI TCCRAC 2 cut(s) 319, 631
MnlI CCTC 6 cut(s) 262, 344, 428, 532, 779, 781
Mox20I TGGCCA 1 cut(s) 339
Mph1103I ATGCAT 1 cut(s) 576
MroXI GAANNNNTTC 1 cut(s) 363
MscI TGGCCA 1 cut(s) 339
MseI TTAA 1 cut(s) 588
Msp20I TGGCCA 1 cut(s) 339
MspCI CTTAAG 1 cut(s) 587
MspI CCGG 2 cut(s) 96, 607
MspR9I CCNGG 1 cut(s) 608
MwoI GCNNNNNNNGC 2 cut(s) 11, 746
NciI CCSGG 1 cut(s) 608
NcoI CCATGG 2 cut(s) 124, 622
NdeII GATC 9 cut(s) 114, 134, 424, 474, 553, 626, 642, 715, 730
NlaIII CATG 7 cut(s) 65, 92, 128, 562, 578, 585, 626
NlaIV GGNNCC 1 cut(s) 241
NmuCI GTSAC 2 cut(s) 254, 532
NsiI ATGCAT 1 cut(s) 576
PceI AGGCCT 1 cut(s) 438
PdmI GAANNNNTTC 1 cut(s) 363
PfeI GAWTC 1 cut(s) 578
PinAI ACCGGT 1 cut(s) 95
PkrI GCNGC 2 cut(s) 13, 778
Ple19I CGATCG 1 cut(s) 556
PleI GAGTC 1 cut(s) 36
PpsI GAGTC 1 cut(s) 36
Ppu21I YACGTR 1 cut(s) 284
PspN4I GGNNCC 1 cut(s) 241
PsuI RGATCY 1 cut(s) 626
PvuI CGATCG 1 cut(s) 556
RsaI GTAC 1 cut(s) 241
RsaNI GTAC 1 cut(s) 240
SaqAI TTAA 1 cut(s) 588
SatI GCNGC 2 cut(s) 12, 777
Sau3AI GATC 9 cut(s) 114, 134, 424, 474, 553, 626, 642, 715, 730
SchI GAGTC 1 cut(s) 37
ScrFI CCNGG 1 cut(s) 608
SduI GDGCHC 1 cut(s) 133
SfaNI GCATC 2 cut(s) 646, 758
SmlI CTYRAG 3 cut(s) 440, 587, 770
SmoI CTYRAG 3 cut(s) 440, 587, 770
Sse9I AATT 2 cut(s) 192, 330
SseBI AGGCCT 1 cut(s) 438
SspI AATATT 1 cut(s) 154
SspMI CTAG 2 cut(s) 306, 808
StuI AGGCCT 1 cut(s) 438
StyD4I CCNGG 1 cut(s) 606
StyI CCWWGG 2 cut(s) 124, 622
TaaI ACNGT 4 cut(s) 322, 344, 400, 619
TaiI ACGT 3 cut(s) 286, 451, 642
TaqI TCGA 3 cut(s) 133, 210, 552
TasI AATT 2 cut(s) 192, 330
TfiI GAWTC 1 cut(s) 578
Tru1I TTAA 1 cut(s) 588
Tru9I TTAA 1 cut(s) 588
TscAI CASTG 1 cut(s) 666
TseFI GTSAC 2 cut(s) 254, 532
TseI GCWGC 2 cut(s) 11, 776
Tsp45I GTSAC 2 cut(s) 254, 532
TspDTI ATGAA 4 cut(s) 105, 372, 575, 591
TspGWI ACGGA 1 cut(s) 551
TspRI CASTG 1 cut(s) 666
Vha464I CTTAAG 1 cut(s) 587
XmnI GAANNNNTTC 1 cut(s) 363
XspI CTAG 2 cut(s) 306, 808
Zsp2I ATGCAT 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.