Rh2DG082200

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000515
Physical Location & Seq
Reverse (-)
6459137 .. 6462052
2916 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG082200.1

Sequence Viewer

Length: 798 bp
ATGGCAGAGGCTGCACAGATGCTAGTGAGCCAGAAGCAGAAAATGATTGTAACCAACAATCATGGTGAAAAGCTTGTCGGCTTATTACATGAAACCGGTTCAAGGGAGATTGTGATCTTGTGCCATCCAGCCGGAGCCTCCAAGGACCACTATATTATAGAGAACCTTGCTGTTGCATTGGAAAAGCAAGGAATTAGTTCCTTCCGTTTTGATTTCGCTGGAAATGGAGAAAGTGAAGGCGCCTTTCAGCTTGGTCACTTGAGGAGAGAAGCTGATGACTTGCATGCTGTAATCCAACACTTCTCTGGATCAAACCATGTAGTTAATGCAATTTTCGGGCACAGTAAAGGAGGCGCTGATGTGCTCCTGTATGCTTCAAAGTATCATGACATTCCTACGGTTGTCGAAGCTTCTGCACGCTATGACCTAAAGAAAGGCATTGAAAAACATCATGGGAAAGACTTTATGGAGAAGATCAAAAAGGAAGGATTCATTGATATTGGAAGTCGTAAATTTCGGGTGACTGAGGAAAGCTTGATGGACATCCTAAGCACTGATATGCGTGAATCGTGCCTCAAGATTGACAAAGATTGCCGGGTGCTAATAGTCCATGGATCTGCTGATGAGATCACTCCTGTTGAAGATGCATTTGAGTTTGCCAAGATCATACCTAACCACAAAATACATATTATAGAAGGAGCTGATCATTGTTACAAATCGCATCAAGCCGAGTTAGACACAGTTGTTGTGGACTTCATAAAAGCAGCTCTGCAGCAGGACAAGGCTTCTTCCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

265

Amino Acids

29.4

Weight (kDa)

6.0

Isoelectric Point (pI)

32.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 35 - 187 5.8e-12 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47590 AT3G47590
fragaria_vesca FvH4_1g07650 FvH4_1g07670 FvH4_1g07671 FvH4_1g07680 FvH4_1g07690 FvH4_1g07700 FvH4_1g07700
malus_domestica MD02G1080900.v1.1 MD02G1081000.v1.1 MD02G1081300.v1.1 MD02G1081400.v1.1 MD02G1094000.v1.1 MD15G1208700.v1.1
prunus_persica Prupe.7G208200_v2.0.a1 Prupe.7G208200_v2.0.a1
pyrus_communis pycom02g07440 pycom15g18480
rosa_chinensis RchiOBHm_Chr2g0093511 RchiOBHm_Chr2g0093521 RchiOBHm_Chr2g0093531 RchiOBHm_Chr2g0093541 RchiOBHm_Chr2g0093551 RchiOBHm_Chr2g0093561
rosa_laevigata RLG00000016413 RLG00000016414 RLG00000016415 RLG00000016416 RLG00000016417
rosa_multiflora Rmu_sc0004423.1_g000003 Rmu_sc0004423.1_g000004 Rmu_sc0004423.1_g000005 Rmu_sc0004423.1_g000006 Rmu_sc0006964.1_g000003 Rmu_sc0039672.1_g000001 Rmu_sc0039672.1_g000003
rosa_roxburghii Rroxscaffold_2G00147990 Rroxscaffold_2G00148000 Rroxscaffold_2G00148010 Rroxscaffold_2G00148020 Rroxscaffold_4G00313540
rosa_rugosa Rorug01G0140600.1 Rorug01G0140700.1 Rorug02G0037000 Rorug02G0037100 Rorug02G0037200 Rorug02G0037300 Rorug02G0037400 Rorug02G0037500 Rorug02G0037600
rosa_samantha Rh2AG083400 Rh2AG083500 Rh2AG083700 Rh2AG083800 Rh2AG083900 Rh2AG084000 Rh2BG084200 Rh2BG084300 Rh2BG084400 Rh2BG084500 Rh2BG084600 Rh2CG086300 Rh2CG086400 Rh2CG086500 Rh2CG086700 Rh2CG086800 Rh2CG086900 Rh2DG082200 Rh2DG082300 Rh2DG082400 Rh2DG082500 Rh2DG082600 Rh2DG082700
rosa_wichuraiana Rw1G013200 Rw2G006360 Rw2G006370 Rw2G006380 Rw2G006390 Rw2G006400 Rw2G007130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 239
AclWI GGATC 2 cut(s) 316, 622
AcsI RAATTY 1 cut(s) 512
AcyI GRCGYC 1 cut(s) 240
AfiI CCNNNNNNNGG 1 cut(s) 102
AgeI ACCGGT 1 cut(s) 95
AgsI TTSAA 4 cut(s) 102, 378, 443, 641
AluBI AGCT 7 cut(s) 73, 250, 272, 410, 534, 701, 767
AluI AGCT 7 cut(s) 73, 250, 272, 410, 534, 701, 767
Alw21I GWGCWC 1 cut(s) 366
AlwI GGATC 2 cut(s) 316, 622
AlwNI CAGNNNCTG 1 cut(s) 11
ApeKI GCWGC 3 cut(s) 11, 764, 772
ApoI RAATTY 1 cut(s) 512
AsiGI ACCGGT 1 cut(s) 95
Asp700I GAANNNNTTC 1 cut(s) 196
AspLEI GCGC 2 cut(s) 242, 356
AspS9I GGNCC 1 cut(s) 145
AsuC2I CCSGG 1 cut(s) 596
AsuHPI GGTGA 2 cut(s) 77, 532
AvaII GGWCC 1 cut(s) 145
BaeGI GKGCMC 1 cut(s) 342
BanI GGYRCC 1 cut(s) 239
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 2 cut(s) 776, 784
BccI CCATC 2 cut(s) 132, 532
BcgI CGANNNNNNTGC 2 cut(s) 395, 429
BclI TGATCA 1 cut(s) 703
BcnI CCSGG 1 cut(s) 596
BfaI CTAG 2 cut(s) 23, 796
BfmI CTRYAG 1 cut(s) 770
BfoI RGCGCY 2 cut(s) 243, 357
BisI GCNGC 3 cut(s) 12, 765, 773
BlsI GCNGC 3 cut(s) 13, 766, 774
Bme1390I CCNGG 1 cut(s) 596
Bme18I GGWCC 1 cut(s) 145
BmgT120I GGNCC 1 cut(s) 145
BmiI GGNNCC 2 cut(s) 136, 241
BmrFI CCNGG 1 cut(s) 596
BmsI GCATC 3 cut(s) 9, 634, 730
Bpu10I CCTNAGC 1 cut(s) 548
BpuEI CTTGAG 2 cut(s) 280, 560
BpuMI CCSGG 1 cut(s) 596
BsaBI GATNNNNATC 2 cut(s) 113, 542
BsaHI GRCGYC 1 cut(s) 240
BsaJI CCNNGG 2 cut(s) 141, 610
BsaWI WCCGGW 1 cut(s) 95
Bsc4I CCNNNNNNNGG 1 cut(s) 102
Bse118I RCCGGY 1 cut(s) 95
Bse8I GATNNNNATC 2 cut(s) 113, 542
BseDI CCNNGG 2 cut(s) 141, 610
BseGI GGATG 2 cut(s) 124, 543
BseJI GATNNNNATC 2 cut(s) 113, 542
BseLI CCNNNNNNNGG 1 cut(s) 102
BseMII CTCAG 1 cut(s) 516
BseRI GAGGAG 1 cut(s) 277
BseSI GKGCMC 1 cut(s) 342
BseXI GCAGC 2 cut(s) 776, 784
BsgI GTGCAG 1 cut(s) 399
BshNI GGYRCC 1 cut(s) 239
BshTI ACCGGT 1 cut(s) 95
BsiHKAI GWGCWC 1 cut(s) 366
BsiSI CCGG 3 cut(s) 96, 132, 595
BslI CCNNNNNNNGG 1 cut(s) 102
Bsp1286I GDGCHC 2 cut(s) 342, 366
Bsp143I GATC 7 cut(s) 114, 308, 474, 614, 627, 663, 703
Bsp19I CCATGG 1 cut(s) 610
BspCNI CTCAG 1 cut(s) 517
BspHI TCATGA 1 cut(s) 385
BspLI GGNNCC 2 cut(s) 136, 241
BspMAI CTGCAG 1 cut(s) 774
BspPI GGATC 2 cut(s) 316, 622
BspT107I GGYRCC 1 cut(s) 239
BsrFI RCCGGY 1 cut(s) 95
BssAI RCCGGY 1 cut(s) 95
BssECI CCNNGG 2 cut(s) 141, 610
BssMI GATC 7 cut(s) 114, 308, 474, 614, 627, 663, 703
BssNI GRCGYC 1 cut(s) 240
BssT1I CCWWGG 2 cut(s) 141, 610
Bst4CI ACNGT 3 cut(s) 344, 400, 742
BstACI GRCGYC 1 cut(s) 240
BstAPI GCANNNNNTGC 1 cut(s) 11
BstC8I GCNNGC 2 cut(s) 285, 418
BstDEI CTNAG 2 cut(s) 525, 548
BstDSI CCRYGG 1 cut(s) 610
BstF5I GGATG 2 cut(s) 124, 543
BstH2I RGCGCY 2 cut(s) 243, 357
BstHHI GCGC 2 cut(s) 242, 356
BstKTI GATC 7 cut(s) 117, 311, 477, 617, 630, 666, 706
BstMBI GATC 7 cut(s) 114, 308, 474, 614, 627, 663, 703
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNSI RCATGY 1 cut(s) 287
BstSCI CCNGG 1 cut(s) 594
BstSFI CTRYAG 1 cut(s) 770
BstSLI GKGCMC 1 cut(s) 342
BstV1I GCAGC 2 cut(s) 776, 784
BstX2I RGATCY 1 cut(s) 614
BstYI RGATCY 1 cut(s) 614
BtgI CCRYGG 1 cut(s) 610
BtsCI GGATG 2 cut(s) 124, 543
BtsIMutI CAGTG 1 cut(s) 552
Cac8I GCNNGC 2 cut(s) 285, 418
CaiI CAGNNNCTG 1 cut(s) 11
CciI TCATGA 1 cut(s) 385
CfoI GCGC 2 cut(s) 242, 356
Cfr10I RCCGGY 1 cut(s) 95
Cfr13I GGNCC 1 cut(s) 145
CspAI ACCGGT 1 cut(s) 95
CviAII CATG 7 cut(s) 62, 89, 284, 317, 386, 452, 611
DdeI CTNAG 2 cut(s) 525, 548
DinI GGCGCC 1 cut(s) 241
DpnI GATC 7 cut(s) 116, 310, 476, 616, 629, 665, 705
DpnII GATC 7 cut(s) 114, 308, 474, 614, 627, 663, 703
Eco130I CCWWGG 2 cut(s) 141, 610
Eco47I GGWCC 1 cut(s) 145
EcoT14I CCWWGG 2 cut(s) 141, 610
EcoT22I ATGCAT 1 cut(s) 649
EgeI GGCGCC 1 cut(s) 241
EheI GGCGCC 1 cut(s) 241
ErhI CCWWGG 2 cut(s) 141, 610
FaeI CATG 7 cut(s) 65, 92, 287, 320, 389, 455, 614
FatI CATG 7 cut(s) 61, 88, 283, 316, 385, 451, 610
FbaI TGATCA 1 cut(s) 703
Fnu4HI GCNGC 3 cut(s) 12, 765, 773
FokI GGATG 2 cut(s) 111, 530
Fsp4HI GCNGC 3 cut(s) 12, 765, 773
FspBI CTAG 2 cut(s) 23, 796
GlaI GCGC 2 cut(s) 241, 355
GluI GCNGC 3 cut(s) 12, 765, 773
HaeII RGCGCY 2 cut(s) 243, 357
HapII CCGG 3 cut(s) 96, 132, 595
HhaI GCGC 2 cut(s) 242, 356
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 7 cut(s) 65, 92, 287, 320, 389, 455, 614
Hin6I GCGC 2 cut(s) 240, 354
HinP1I GCGC 2 cut(s) 240, 354
HindIII AAGCTT 3 cut(s) 71, 408, 532
HinfI GANTC 2 cut(s) 489, 566
HpaII CCGG 3 cut(s) 96, 132, 595
HphI GGTGA 2 cut(s) 77, 532
Hpy166II GTNNAC 1 cut(s) 751
Hpy188III TCNNGA 3 cut(s) 306, 386, 577
Hpy8I GTNNAC 1 cut(s) 751
HpyAV CCTTC 4 cut(s) 211, 230, 479, 689
HpyCH4III ACNGT 3 cut(s) 344, 400, 742
HpyCH4V TGCA 7 cut(s) 14, 176, 283, 329, 416, 647, 772
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 2 cut(s) 525, 548
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 7 cut(s) 65, 92, 287, 320, 389, 455, 614
HspAI GCGC 2 cut(s) 240, 354
KasI GGCGCC 1 cut(s) 239
Ksp22I TGATCA 1 cut(s) 703
Kzo9I GATC 7 cut(s) 114, 308, 474, 614, 627, 663, 703
LmnI GCTCC 3 cut(s) 134, 369, 698
Lsp1109I GCAGC 2 cut(s) 776, 784
LweI GCATC 3 cut(s) 9, 634, 730
MaeI CTAG 2 cut(s) 23, 796
MaeIII GTNAC 4 cut(s) 49, 254, 520, 710
MalI GATC 7 cut(s) 116, 310, 476, 616, 629, 665, 705
MboI GATC 7 cut(s) 114, 308, 474, 614, 627, 663, 703
MboII GAAGA 3 cut(s) 484, 653, 780
MflI RGATCY 1 cut(s) 614
MhlI GDGCHC 2 cut(s) 342, 366
MluCI AATT 3 cut(s) 192, 330, 512
Mly113I GGCGCC 1 cut(s) 240
MmeI TCCRAC 1 cut(s) 319
MnlI CCTC 5 cut(s) 148, 255, 344, 520, 584
Mph1103I ATGCAT 1 cut(s) 649
MroXI GAANNNNTTC 1 cut(s) 196
MseI TTAA 1 cut(s) 324
MslI CAYNNNNRTG 1 cut(s) 557
MspI CCGG 3 cut(s) 96, 132, 595
MspR9I CCNGG 1 cut(s) 596
MwoI GCNNNNNNNGC 1 cut(s) 11
NarI GGCGCC 1 cut(s) 240
NciI CCSGG 1 cut(s) 596
NcoI CCATGG 1 cut(s) 610
NdeII GATC 7 cut(s) 114, 308, 474, 614, 627, 663, 703
NlaIII CATG 7 cut(s) 65, 92, 287, 320, 389, 455, 614
NlaIV GGNNCC 2 cut(s) 136, 241
NmeAIII GCCGAG 1 cut(s) 754
NmuCI GTSAC 2 cut(s) 254, 520
NsiI ATGCAT 1 cut(s) 649
NspI RCATGY 1 cut(s) 287
PaeI GCATGC 1 cut(s) 287
PagI TCATGA 1 cut(s) 385
PdmI GAANNNNTTC 1 cut(s) 196
PfeI GAWTC 2 cut(s) 489, 566
PinAI ACCGGT 1 cut(s) 95
PkrI GCNGC 3 cut(s) 13, 766, 774
PluTI GGCGCC 1 cut(s) 243
PspN4I GGNNCC 2 cut(s) 136, 241
PspPI GGNCC 1 cut(s) 145
PstI CTGCAG 1 cut(s) 774
PstNI CAGNNNCTG 1 cut(s) 11
PsuI RGATCY 1 cut(s) 614
RseI CAYNNNNRTG 1 cut(s) 557
SaqAI TTAA 1 cut(s) 324
SatI GCNGC 3 cut(s) 12, 765, 773
Sau3AI GATC 7 cut(s) 114, 308, 474, 614, 627, 663, 703
Sau96I GGNCC 1 cut(s) 145
ScrFI CCNGG 1 cut(s) 596
SduI GDGCHC 2 cut(s) 342, 366
SfaNI GCATC 3 cut(s) 9, 634, 730
SfcI CTRYAG 1 cut(s) 770
SfoI GGCGCC 1 cut(s) 241
SinI GGWCC 1 cut(s) 145
SmiMI CAYNNNNRTG 1 cut(s) 557
SmlI CTYRAG 2 cut(s) 259, 575
SmoI CTYRAG 2 cut(s) 259, 575
SphI GCATGC 1 cut(s) 287
Sse9I AATT 3 cut(s) 192, 330, 512
SspDI GGCGCC 1 cut(s) 239
SspMI CTAG 2 cut(s) 23, 796
StyD4I CCNGG 1 cut(s) 594
StyI CCWWGG 2 cut(s) 141, 610
TaaI ACNGT 3 cut(s) 344, 400, 742
TaqI TCGA 1 cut(s) 405
TasI AATT 3 cut(s) 192, 330, 512
TfiI GAWTC 2 cut(s) 489, 566
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TscAI CASTG 1 cut(s) 559
TseFI GTSAC 2 cut(s) 254, 520
TseI GCWGC 3 cut(s) 11, 764, 772
Tsp45I GTSAC 2 cut(s) 254, 520
TspDTI ATGAA 3 cut(s) 105, 481, 745
TspGWI ACGGA 1 cut(s) 194
TspRI CASTG 1 cut(s) 559
VpaK11BI GGWCC 1 cut(s) 145
XapI RAATTY 1 cut(s) 512
XceI RCATGY 1 cut(s) 287
XcmI CCANNNNNNNNNTGG 1 cut(s) 302
XmnI GAANNNNTTC 1 cut(s) 196
XspI CTAG 2 cut(s) 23, 796
Zsp2I ATGCAT 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.