Prupe.7G208200_v2.0.a1

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0000515
Physical Location & Seq
Forward (+)
19119953 .. 19122734
2782 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G208200.1

Sequence Viewer

Length: 900 bp
ATGCTGCTCTCAAGTTCTTTCACTCCCATAAGCTTCCCTTCACCAAAATTTACTCTCTACCAAAATCGCAGCCCAAAACGAATCTTGAAAATGGCACAATCTGCACACAACCCAGTGGGTCGGCAACAGAAAATCATTGTACCCAACAAACATGATGAAAAACTCGTGGGACTACTACATGAAACCGGTTCTGAGGAGATTGTAATCTTATGTCATGGTTTTCGATCCACCAAGGAAACCACTACTATTACAAACCTTGCCATTGCATTGGAAAATGAAGGAATTAGTGCCTTCCGTTTTGACTTTGCTGGAAATGGGGAGAGTGAAGGCACCTTTCAGTATGGTAACTATTGGAGAGAGGCTGATGACTTGCATGCTGTGATCCAACACTTCTCTGGGGAAAACCGTGCAGCAAGTACAATTCTTGGGCACAGTAAAGGTGGTGATGTGGTGCTCCTGTATGCTTCTAAGTATCATGGCATTCGCACAGTTGTCAATGTTTCTGGACGTTATGATCTCCAGAGAGGTATTGAAGAACGCTTAGGGAAAGACTTTGTGGAAACAATCAAGAAGGAAGGATATCTTGATGTTAAGAATAAGACGGGAGATGTTAGTTATCGTGTGACCGAGGAAAGTTTGATGGATCGCCTGAGTACTGATATGCATGAAGCATGCCTTCAGATTGACAAAGAATGCCGGGTCTTGACAGTCCATGGTTCTGCTGATGAGACCATCCCTGTTGAAGATGCATTAGAGTTTTCGAAGATAATACCAAACCACAAATTACATGTTATAGAAGGGGCTGATCATTGTTACACCTCACATCAGACTGAGTTAGTATCAGTTGTTTTGGACTTCATAAAGGCAGCACTGCAGCAGGACAAGGCTACTTCCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

300

Amino Acids

33.41

Weight (kDa)

5.96

Isoelectric Point (pI)

41.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47590 AT3G47590
fragaria_vesca FvH4_1g07650 FvH4_1g07670 FvH4_1g07671 FvH4_1g07680 FvH4_1g07690 FvH4_1g07700 FvH4_1g07700
malus_domestica MD02G1080900.v1.1 MD02G1081000.v1.1 MD02G1081300.v1.1 MD02G1081400.v1.1 MD02G1094000.v1.1 MD15G1208700.v1.1
prunus_persica Prupe.7G208200_v2.0.a1 Prupe.7G208200_v2.0.a1
pyrus_communis pycom02g07440 pycom15g18480
rosa_chinensis RchiOBHm_Chr2g0093511 RchiOBHm_Chr2g0093521 RchiOBHm_Chr2g0093531 RchiOBHm_Chr2g0093541 RchiOBHm_Chr2g0093551 RchiOBHm_Chr2g0093561
rosa_laevigata RLG00000016413 RLG00000016414 RLG00000016415 RLG00000016416 RLG00000016417
rosa_multiflora Rmu_sc0004423.1_g000003 Rmu_sc0004423.1_g000004 Rmu_sc0004423.1_g000005 Rmu_sc0004423.1_g000006 Rmu_sc0006964.1_g000003 Rmu_sc0039672.1_g000001 Rmu_sc0039672.1_g000003
rosa_roxburghii Rroxscaffold_2G00147990 Rroxscaffold_2G00148000 Rroxscaffold_2G00148010 Rroxscaffold_2G00148020 Rroxscaffold_4G00313540
rosa_rugosa Rorug01G0140600.1 Rorug01G0140700.1 Rorug02G0037000 Rorug02G0037100 Rorug02G0037200 Rorug02G0037300 Rorug02G0037400 Rorug02G0037500 Rorug02G0037600
rosa_samantha Rh2AG083400 Rh2AG083500 Rh2AG083700 Rh2AG083800 Rh2AG083900 Rh2AG084000 Rh2BG084200 Rh2BG084300 Rh2BG084400 Rh2BG084500 Rh2BG084600 Rh2CG086300 Rh2CG086400 Rh2CG086500 Rh2CG086700 Rh2CG086800 Rh2CG086900 Rh2DG082200 Rh2DG082300 Rh2DG082400 Rh2DG082500 Rh2DG082600 Rh2DG082700
rosa_wichuraiana Rw1G013200 Rw2G006360 Rw2G006370 Rw2G006380 Rw2G006390 Rw2G006400 Rw2G007130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 329
AclWI GGATC 3 cut(s) 219, 376, 651
AcsI RAATTY 1 cut(s) 47
AcuI CTGAAG 1 cut(s) 662
AfaI GTAC 3 cut(s) 141, 418, 655
AflIII ACRYGT 1 cut(s) 787
AgeI ACCGGT 1 cut(s) 185
AgsI TTSAA 3 cut(s) 88, 533, 743
AluBI AGCT 1 cut(s) 33
AluI AGCT 1 cut(s) 33
Alw21I GWGCWC 1 cut(s) 456
Alw26I GTCTC 1 cut(s) 722
AlwI GGATC 3 cut(s) 219, 376, 651
ApeKI GCWGC 5 cut(s) 4, 69, 410, 866, 874
ApoI RAATTY 1 cut(s) 47
AsiGI ACCGGT 1 cut(s) 185
AsuC2I CCSGG 1 cut(s) 698
AsuHPI GGTGA 2 cut(s) 33, 455
AsuII TTCGAA 1 cut(s) 761
BaeGI GKGCMC 1 cut(s) 432
BanI GGYRCC 1 cut(s) 329
BauI CACGAG 1 cut(s) 164
Bbv12I GWGCWC 1 cut(s) 456
BbvI GCAGC 4 cut(s) 81, 422, 878, 886
BccI CCATC 2 cut(s) 634, 740
BclI TGATCA 1 cut(s) 805
BcnI CCSGG 1 cut(s) 698
BcoDI GTCTC 1 cut(s) 722
BfmI CTRYAG 1 cut(s) 872
BisI GCNGC 5 cut(s) 5, 70, 411, 867, 875
BlsI GCNGC 5 cut(s) 6, 71, 412, 868, 876
BmcAI AGTACT 1 cut(s) 655
Bme1390I CCNGG 1 cut(s) 698
BmiI GGNNCC 1 cut(s) 331
BmrFI CCNGG 1 cut(s) 698
BmrI ACTGGG 1 cut(s) 107
BmsI GCATC 1 cut(s) 736
BmuI ACTGGG 1 cut(s) 107
BpmI CTGGAG 1 cut(s) 503
Bpu10I CCTNAGC 1 cut(s) 541
Bpu14I TTCGAA 1 cut(s) 761
BpuMI CCSGG 1 cut(s) 698
BsaBI GATNNNNATC 1 cut(s) 203
BsaI GGTCTC 1 cut(s) 722
BsaJI CCNNGG 3 cut(s) 231, 627, 712
BsaWI WCCGGW 1 cut(s) 185
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 1 cut(s) 113
Bse3DI GCAATG 1 cut(s) 261
Bse8I GATNNNNATC 1 cut(s) 203
BseDI CCNNGG 3 cut(s) 231, 627, 712
BseGI GGATG 1 cut(s) 732
BseJI GATNNNNATC 1 cut(s) 203
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 3 cut(s) 183, 641, 822
BseNI ACTGG 1 cut(s) 113
BseRI GAGGAG 1 cut(s) 209
BseSI GKGCMC 1 cut(s) 432
BseXI GCAGC 4 cut(s) 81, 422, 878, 886
BsgI GTGCAG 2 cut(s) 87, 429
BshNI GGYRCC 1 cut(s) 329
BshTI ACCGGT 1 cut(s) 185
BsiHKAI GWGCWC 1 cut(s) 456
BsiSI CCGG 2 cut(s) 186, 697
BslFI GGGAC 1 cut(s) 183
BsmAI GTCTC 1 cut(s) 722
BsmFI GGGAC 1 cut(s) 183
BsmI GAATGC 2 cut(s) 480, 698
Bso31I GGTCTC 1 cut(s) 722
Bsp119I TTCGAA 1 cut(s) 761
Bsp1286I GDGCHC 2 cut(s) 432, 456
Bsp143I GATC 5 cut(s) 224, 381, 514, 643, 805
Bsp19I CCATGG 1 cut(s) 712
BspCNI CTCAG 3 cut(s) 184, 642, 823
BspLI GGNNCC 1 cut(s) 331
BspMAI CTGCAG 1 cut(s) 876
BspPI GGATC 3 cut(s) 219, 376, 651
BspT104I TTCGAA 1 cut(s) 761
BspT107I GGYRCC 1 cut(s) 329
BspTNI GGTCTC 1 cut(s) 722
BsrDI GCAATG 1 cut(s) 261
BsrFI RCCGGY 1 cut(s) 185
BsrI ACTGG 1 cut(s) 113
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 3 cut(s) 231, 627, 712
BssMI GATC 5 cut(s) 224, 381, 514, 643, 805
BssSI CACGAG 1 cut(s) 164
BssT1I CCWWGG 2 cut(s) 231, 712
Bst2BI CACGAG 1 cut(s) 164
Bst4CI ACNGT 4 cut(s) 407, 434, 490, 709
BstAPI GCANNNNNTGC 1 cut(s) 101
BstBI TTCGAA 1 cut(s) 761
BstC8I GCNNGC 2 cut(s) 375, 673
BstDEI CTNAG 5 cut(s) 192, 468, 541, 650, 831
BstDSI CCRYGG 1 cut(s) 712
BstF5I GGATG 1 cut(s) 732
BstKTI GATC 5 cut(s) 227, 384, 517, 646, 808
BstMAI GTCTC 1 cut(s) 722
BstMBI GATC 5 cut(s) 224, 381, 514, 643, 805
BstMWI GCNNNNNNNGC 1 cut(s) 101
BstNSI RCATGY 3 cut(s) 377, 675, 791
BstSCI CCNGG 1 cut(s) 696
BstSFI CTRYAG 1 cut(s) 872
BstSLI GKGCMC 1 cut(s) 432
BstV1I GCAGC 4 cut(s) 81, 422, 878, 886
BstXI CCANNNNNNTGG 1 cut(s) 268
BtgI CCRYGG 1 cut(s) 712
BtsCI GGATG 1 cut(s) 732
BtsI GCAGTG 1 cut(s) 869
BtsIMutI CAGTG 2 cut(s) 120, 869
Cac8I GCNNGC 2 cut(s) 375, 673
Cfr10I RCCGGY 1 cut(s) 185
Csp6I GTAC 3 cut(s) 140, 417, 654
CspAI ACCGGT 1 cut(s) 185
CviAII CATG 9 cut(s) 152, 179, 215, 374, 476, 665, 672, 713, 788
CviJI RGCY 5 cut(s) 33, 72, 362, 803, 887
CviKI_1 RGCY 5 cut(s) 33, 72, 362, 803, 887
CviQI GTAC 3 cut(s) 140, 417, 654
DdeI CTNAG 5 cut(s) 192, 468, 541, 650, 831
DpnI GATC 5 cut(s) 226, 383, 516, 645, 807
DpnII GATC 5 cut(s) 224, 381, 514, 643, 805
Eco130I CCWWGG 2 cut(s) 231, 712
Eco31I GGTCTC 1 cut(s) 722
Eco32I GATATC 1 cut(s) 581
Eco57I CTGAAG 1 cut(s) 662
EcoRV GATATC 1 cut(s) 581
EcoT14I CCWWGG 2 cut(s) 231, 712
EcoT22I ATGCAT 2 cut(s) 666, 751
ErhI CCWWGG 2 cut(s) 231, 712
FaeI CATG 9 cut(s) 155, 182, 218, 377, 479, 668, 675, 716, 791
FalI AAGNNNNNCTT 6 cut(s) 22, 54, 567, 599, 660, 692
FaqI GGGAC 1 cut(s) 183
FatI CATG 9 cut(s) 151, 178, 214, 373, 475, 664, 671, 712, 787
FbaI TGATCA 1 cut(s) 805
Fnu4HI GCNGC 5 cut(s) 5, 70, 411, 867, 875
FokI GGATG 1 cut(s) 719
Fsp4HI GCNGC 5 cut(s) 5, 70, 411, 867, 875
GluI GCNGC 5 cut(s) 5, 70, 411, 867, 875
GsuI CTGGAG 1 cut(s) 503
HapII CCGG 2 cut(s) 186, 697
Hin1II CATG 9 cut(s) 155, 182, 218, 377, 479, 668, 675, 716, 791
HindIII AAGCTT 1 cut(s) 31
HinfI GANTC 1 cut(s) 81
HpaII CCGG 2 cut(s) 186, 697
HphI GGTGA 2 cut(s) 33, 455
Hpy188I TCNGA 3 cut(s) 193, 681, 828
Hpy188III TCNNGA 6 cut(s) 85, 504, 520, 568, 584, 703
HpyAV CCTTC 8 cut(s) 48, 272, 301, 320, 565, 569, 686, 791
HpyCH4III ACNGT 4 cut(s) 407, 434, 490, 709
HpyCH4IV ACGT 1 cut(s) 508
HpyCH4V TGCA 7 cut(s) 104, 266, 373, 410, 664, 749, 874
HpyF10VI GCNNNNNNNGC 1 cut(s) 101
HpyF3I CTNAG 5 cut(s) 192, 468, 541, 650, 831
HpySE526I ACGT 1 cut(s) 508
Hsp92II CATG 9 cut(s) 155, 182, 218, 377, 479, 668, 675, 716, 791
Ksp22I TGATCA 1 cut(s) 805
Kzo9I GATC 5 cut(s) 224, 381, 514, 643, 805
LmnI GCTCC 1 cut(s) 459
Lsp1109I GCAGC 4 cut(s) 81, 422, 878, 886
LweI GCATC 1 cut(s) 736
MaeII ACGT 1 cut(s) 508
MaeIII GTNAC 3 cut(s) 344, 622, 812
MalI GATC 5 cut(s) 226, 383, 516, 645, 807
MboI GATC 5 cut(s) 224, 381, 514, 643, 805
MboII GAAGA 3 cut(s) 545, 755, 775
MhlI GDGCHC 2 cut(s) 432, 456
MluCI AATT 4 cut(s) 47, 282, 420, 782
MmeI TCCRAC 1 cut(s) 409
MnlI CCTC 5 cut(s) 187, 352, 518, 622, 829
Mph1103I ATGCAT 2 cut(s) 666, 751
MseI TTAA 1 cut(s) 591
MspI CCGG 2 cut(s) 186, 697
MspR9I CCNGG 1 cut(s) 698
Mva1269I GAATGC 2 cut(s) 480, 698
MwoI GCNNNNNNNGC 1 cut(s) 101
NciI CCSGG 1 cut(s) 698
NcoI CCATGG 1 cut(s) 712
NdeII GATC 5 cut(s) 224, 381, 514, 643, 805
NlaIII CATG 9 cut(s) 155, 182, 218, 377, 479, 668, 675, 716, 791
NlaIV GGNNCC 1 cut(s) 331
NmuCI GTSAC 1 cut(s) 622
NsiI ATGCAT 2 cut(s) 666, 751
NspI RCATGY 3 cut(s) 377, 675, 791
NspV TTCGAA 1 cut(s) 761
PaeI GCATGC 2 cut(s) 377, 675
PciI ACATGT 1 cut(s) 787
PctI GAATGC 2 cut(s) 480, 698
PfeI GAWTC 1 cut(s) 81
PinAI ACCGGT 1 cut(s) 185
PkrI GCNGC 5 cut(s) 6, 71, 412, 868, 876
PscI ACATGT 1 cut(s) 787
PspN4I GGNNCC 1 cut(s) 331
PstI CTGCAG 1 cut(s) 876
RsaI GTAC 3 cut(s) 141, 418, 655
RsaNI GTAC 3 cut(s) 140, 417, 654
SaqAI TTAA 1 cut(s) 591
SatI GCNGC 5 cut(s) 5, 70, 411, 867, 875
Sau3AI GATC 5 cut(s) 224, 381, 514, 643, 805
ScaI AGTACT 1 cut(s) 655
ScrFI CCNGG 1 cut(s) 698
SduI GDGCHC 2 cut(s) 432, 456
SetI ASST 7 cut(s) 35, 258, 335, 442, 511, 529, 821
SfaNI GCATC 1 cut(s) 736
SfcI CTRYAG 1 cut(s) 872
SfuI TTCGAA 1 cut(s) 761
SmlI CTYRAG 1 cut(s) 10
SmoI CTYRAG 1 cut(s) 10
SphI GCATGC 2 cut(s) 377, 675
Sse9I AATT 4 cut(s) 47, 282, 420, 782
StyD4I CCNGG 1 cut(s) 696
StyI CCWWGG 2 cut(s) 231, 712
TaaI ACNGT 4 cut(s) 407, 434, 490, 709
TaiI ACGT 1 cut(s) 511
TaqI TCGA 2 cut(s) 223, 761
TaqII GACCGA 1 cut(s) 641
TasI AATT 4 cut(s) 47, 282, 420, 782
TatI WGTACW 2 cut(s) 416, 653
TfiI GAWTC 1 cut(s) 81
Tru1I TTAA 1 cut(s) 591
Tru9I TTAA 1 cut(s) 591
TscAI CASTG 2 cut(s) 120, 876
TseFI GTSAC 1 cut(s) 622
TseI GCWGC 5 cut(s) 4, 69, 410, 866, 874
Tsp45I GTSAC 1 cut(s) 622
TspDTI ATGAA 5 cut(s) 171, 195, 291, 681, 847
TspGWI ACGGA 1 cut(s) 284
TspRI CASTG 2 cut(s) 120, 876
XapI RAATTY 1 cut(s) 47
XceI RCATGY 3 cut(s) 377, 675, 791
XcmI CCANNNNNNNNNTGG 1 cut(s) 392
ZrmI AGTACT 1 cut(s) 655
Zsp2I ATGCAT 2 cut(s) 666, 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.