Rorug02G0037000

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000515
Physical Location & Seq
Forward (+)
2901237 .. 2903453
2217 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0037000.1

Sequence Viewer

Length: 2217 bp
ATGGTGTTTTCGTTTTCATCTTCGTTGCTGACCACTCCACCCTCCACCCTTCACGTCCTCCCCGCCTCCGATCCTCCGTACAACCTCCTCCAAACCCACCCATCTCTCATTCTCCTCTCCAAATGCAAAAGCATCAAAAATCTCAAACAAGTCCACACCCACATCATCAAGACCGGCCTCCACAACACCCACTTTGCTATGAGCAAGCTCATCGAGTTTTGCGCCGTTTCGCAATTTGGGGACCTCTCCTATGCCCTGTCGGTCTTCGAATGCGTTGAGAATCCGAACCAGATTATTTGGAACACAATTATTCGAGGCTATTCCTTGAGCTCGAAACCATTACAGGCCGTGGAGTTCTATGTAAGGATGATTCTTTCGGGCGCTGAACCCAATTCTTACACATTCCCTTCTGTTTTGAAGTCTTGTGCGAGAGTTGGGGCAGCTCAGGAAGGGAAGCAGATCCATGGGCATGTGTTGAAGCATGGAGTTGGCTCTGATGCGTTTGTGCACACTTCTCTGATCAACATGTATGCGAAAAGTGGTGAATTGGACAATGCAAGGTTGGTTTTTGATAAGAGTCATCTGAGAGATGCGGTTTCGTTTACTGCTTTGATTACTGGCTATGTTTCAAGGGGTTGTATGGATGATGCCCGCAGCTTGTTTGATGAAATCTTAGTAAGAGATGCGGTGTCTTGGAATGCAATGATTTCGGGGTATGCTCAGAGCGGTCGGTTTGAGGAAGCGTTGGCTTTGTTTTCGGATATGCGAAAAGCAAATGTGGTTCCTAATGAGAGTACTATGCTTACTGTGCTTTCTGCTTGTGCTCAGTCGGGGTCACTTGAATTGGGAAAATGGGTTGCTTCTTGGATTGAAGACCAGGGACTTGGCTCAAATGTTAGGCTTGTTAATGCACTTATTGATATGTACTCAAAGTGTGGTGCTTTGGACACAGCTAGAGGTTTATTTGATGGTATGCAACAGAGGGATGTGATCTCATGGAATGTGATGATTGGTGGTTATACACATGAGAGCCGGTACAAGGAAGCCTTGGCACTCTTTCGGTTAATGCTACGTTCAAATGCAGAGCCTAATGATGTCACATTCTTGGGCATTCTTCCAGCTTGTGCTCATTTGGGAGCTCTTGACCTTGGCAAATGGATTCATGTTTACATAGATAATAAGTTGCAGTGTTTGACCAATACATCTCTCCAGACCAGTCTCATAGACATGTATGCAAAGTGCGGAAACATCGAGGCAGCAAAACAAGTCTTTGATGGTATGGAAGCCAAAAGCTTGGCCTCTTGGAATGCAATGATAACTGGCTTAGCAATGCATGGGAAAGCAAATGCAGCACTTGAGCTTTTCTCGAAGATGGCTGATGAAGGATTCAAACCAGATGATATCACATTTGTGGGAGTTTTTTCGGCTTGCAACCATGGTGGTCTAGTAGACCTTGGACGCCAATATTTTAGTTCCATGACCAAAGACTACCATATTTCACCGCAACTACAACACTATGGATGTATGATAGATCTTCTAGGACGAGCTGGGCTACTTGATGAAGCAGAGGCCCTAATGAAGACCATGGAGATGAAGCCAGATGGTGCTGTTTGGGGTTCTCTTCTTGGGGCTTGTAGAATCCATAAGCGTGTAGAGATGGGTGAATATGTTGCCAAACATGTGTTCGAATTGGAGCCTGAAAATGCTGGGGCTCATGTGCTGCTATCAAATATCTATGCAAGTGCTGGTAGGTGGGATGATGTAGCCAGAATAAGAACCACGCTGAACGATACGGGAATCAAGAAAGTTCCTGGCAGTACTTCTATTGAGCTGGATAGTGTTGTTCATGAGTTTCTTGTTGGTGACAAAGTGCATCCTTTAAGTGAAGAAATCTATGAGATGTTGAAAGAAATGGATAGGCTTTTGGATATGGCTGGTTTTGTGCCTGATACATCTGAGGTACTCTATGATATGGATGAGGAATGGAAGGAAGGAATTTTGAGTCATCATAGTGAGAAGTTGGCCATTGCTTTTGGTTTGATCAGCACAAAGCCAGGCACAACTATTCGAATTGTAAAGAATCTTCGAGTATGTGGAAATTGTCATTCAGCTACAAAGCTGATATCCAAGATCTTCAATAGGGAAATTATTGCAAGAGATAGGAATCGCTTCCACCATTTTAGAGATGGTTCTTGTTCATGTAATGACAATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

738

Amino Acids

81.65

Weight (kDa)

6.16

Isoelectric Point (pI)

39.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 95 - 144 1.4e-08 PPR repeat family
PPR_1 PF12854 226 - 256 2e-06 PPR repeat
PPR_2 PF13041 228 - 275 3.5e-14 PPR repeat family
PPR PF01535 230 - 259 4.3e-10 PPR repeat
PPR_2 PF13041 328 - 377 1.1e-10 PPR repeat family
PPR PF01535 331 - 359 5.6e-06 PPR repeat
PPR_2 PF13041 433 - 477 1.2e-10 PPR repeat family
PPR PF01535 434 - 463 3.7e-06 PPR repeat
E_motif PF20431 548 - 610 6.9e-20 E motif
Eplus_motif PF20430 615 - 637 1.4e-07 E+ motif
DYW_deaminase PF14432 646 - 738 1.8e-40 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47590 AT3G47590
fragaria_vesca FvH4_1g07650 FvH4_1g07670 FvH4_1g07671 FvH4_1g07680 FvH4_1g07690 FvH4_1g07700 FvH4_1g07700
malus_domestica MD02G1080900.v1.1 MD02G1081000.v1.1 MD02G1081300.v1.1 MD02G1081400.v1.1 MD02G1094000.v1.1 MD15G1208700.v1.1
prunus_persica Prupe.7G208200_v2.0.a1 Prupe.7G208200_v2.0.a1
pyrus_communis pycom02g07440 pycom15g18480
rosa_chinensis RchiOBHm_Chr2g0093511 RchiOBHm_Chr2g0093521 RchiOBHm_Chr2g0093531 RchiOBHm_Chr2g0093541 RchiOBHm_Chr2g0093551 RchiOBHm_Chr2g0093561
rosa_laevigata RLG00000016413 RLG00000016414 RLG00000016415 RLG00000016416 RLG00000016417
rosa_multiflora Rmu_sc0004423.1_g000003 Rmu_sc0004423.1_g000004 Rmu_sc0004423.1_g000005 Rmu_sc0004423.1_g000006 Rmu_sc0006964.1_g000003 Rmu_sc0039672.1_g000001 Rmu_sc0039672.1_g000003
rosa_roxburghii Rroxscaffold_2G00147990 Rroxscaffold_2G00148000 Rroxscaffold_2G00148010 Rroxscaffold_2G00148020 Rroxscaffold_4G00313540
rosa_rugosa Rorug01G0140600.1 Rorug01G0140700.1 Rorug02G0037000 Rorug02G0037100 Rorug02G0037200 Rorug02G0037300 Rorug02G0037400 Rorug02G0037500 Rorug02G0037600
rosa_samantha Rh2AG083400 Rh2AG083500 Rh2AG083700 Rh2AG083800 Rh2AG083900 Rh2AG084000 Rh2BG084200 Rh2BG084300 Rh2BG084400 Rh2BG084500 Rh2BG084600 Rh2CG086300 Rh2CG086400 Rh2CG086500 Rh2CG086700 Rh2CG086800 Rh2CG086900 Rh2DG082200 Rh2DG082300 Rh2DG082400 Rh2DG082500 Rh2DG082600 Rh2DG082700
rosa_wichuraiana Rw1G013200 Rw2G006360 Rw2G006370 Rw2G006380 Rw2G006390 Rw2G006400 Rw2G007130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 726
AccI GTMKAC 1 cut(s) 1449
AciI CCGC 7 cut(s) 63, 593, 652, 686, 726, 1242, 1502
AclWI GGATC 2 cut(s) 65, 454
AcoI YGGCCR 1 cut(s) 2022
AcsI RAATTY 1 cut(s) 1995
AcyI GRCGYC 1 cut(s) 1459
AfaI GTAC 6 cut(s) 80, 796, 926, 1037, 1819, 1962
AfiI CCNNNNNNNGG 1 cut(s) 1039
AflIII ACRYGT 3 cut(s) 525, 1227, 1678
AgsI TTSAA 9 cut(s) 418, 478, 630, 842, 872, 1077, 1390, 1906, 2137
AjiI CACGTC 1 cut(s) 55
AjnI CCWGG 3 cut(s) 876, 1810, 2053
AjuI GAANNNNNNNTTGG 2 cut(s) 1667, 1699
AleI CACNNNNGTG 1 cut(s) 1409
Alw21I GWGCWC 5 cut(s) 332, 510, 826, 1129, 1141
Alw26I GTCTC 1 cut(s) 1223
Alw44I GTGCAC 1 cut(s) 506
AlwI GGATC 2 cut(s) 65, 454
AoxI GGCC 5 cut(s) 175, 345, 1296, 1569, 2022
ApaLI GTGCAC 1 cut(s) 506
ApeKI GCWGC 5 cut(s) 440, 654, 1256, 1349, 1720
ApoI RAATTY 1 cut(s) 1995
Asp700I GAANNNNTTC 1 cut(s) 2168
AspLEI GCGC 2 cut(s) 224, 383
AspS9I GGNCC 2 cut(s) 241, 1570
AsuHPI GGTGA 4 cut(s) 554, 1491, 1673, 1874
AsuII TTCGAA 3 cut(s) 267, 1686, 2068
AvaII GGWCC 1 cut(s) 241
BaeGI GKGCMC 1 cut(s) 510
BalI TGGCCA 1 cut(s) 2024
BanII GRGCYC 3 cut(s) 332, 1141, 1714
BarI GAAGNNNNNNTAC 2 cut(s) 2067, 2099
BbsI GAAGAC 3 cut(s) 256, 879, 1586
Bbv12I GWGCWC 5 cut(s) 332, 510, 826, 1129, 1141
BbvI GCAGC 5 cut(s) 452, 666, 1268, 1361, 1707
BccI CCATC 7 cut(s) 109, 962, 1268, 1366, 1595, 1651, 2180
BceAI ACGGC 2 cut(s) 209, 332
BcgI CGANNNNNNTGC 4 cut(s) 193, 227, 690, 724
BciT130I CCWGG 3 cut(s) 878, 1812, 2055
BclI TGATCA 2 cut(s) 519, 2040
BcoDI GTCTC 1 cut(s) 1223
BfaI CTAG 3 cut(s) 954, 1445, 1538
BfoI RGCGCY 1 cut(s) 384
BglII AGATCT 2 cut(s) 1531, 2130
BisI GCNGC 5 cut(s) 441, 655, 1257, 1350, 1721
BlpI GCTNAGC 1 cut(s) 1324
BlsI GCNGC 5 cut(s) 442, 656, 1258, 1351, 1722
BmcAI AGTACT 2 cut(s) 796, 1819
Bme1390I CCNGG 3 cut(s) 878, 1812, 2055
Bme18I GGWCC 1 cut(s) 241
BmgBI CACGTC 1 cut(s) 55
BmgT120I GGNCC 2 cut(s) 241, 1570
BmiI GGNNCC 3 cut(s) 242, 783, 1695
BmrFI CCNGG 3 cut(s) 878, 1812, 2055
BmsI GCATC 6 cut(s) 141, 487, 580, 637, 673, 1882
BpiI GAAGAC 3 cut(s) 256, 879, 1586
BplI GAGNNNNNCTC 2 cut(s) 1349, 1381
BpmI CTGGAG 1 cut(s) 1193
Bpu10I CCTNAGC 1 cut(s) 444
Bpu1102I GCTNAGC 1 cut(s) 1324
Bpu14I TTCGAA 3 cut(s) 267, 1686, 2068
BpuEI CTTGAG 2 cut(s) 346, 1376
BsaBI GATNNNNATC 1 cut(s) 2163
BsaHI GRCGYC 1 cut(s) 1459
BsaJI CCNNGG 8 cut(s) 348, 463, 877, 1047, 1147, 1435, 1453, 1584
Bsc4I CCNNNNNNNGG 1 cut(s) 1039
Bse118I RCCGGY 2 cut(s) 173, 1032
Bse1I ACTGG 3 cut(s) 622, 1215, 1324
Bse3DI GCAATG 4 cut(s) 708, 1317, 1335, 2025
Bse8I GATNNNNATC 1 cut(s) 2163
BseBI CCWGG 3 cut(s) 878, 1812, 2055
BseDI CCNNGG 8 cut(s) 348, 463, 877, 1047, 1147, 1435, 1453, 1584
BseGI GGATG 7 cut(s) 372, 649, 991, 1526, 1762, 1873, 1981
BseJI GATNNNNATC 1 cut(s) 2163
BseLI CCNNNNNNNGG 1 cut(s) 1039
BseMI GCAATG 4 cut(s) 708, 1317, 1335, 2025
BseMII CTCAG 5 cut(s) 458, 575, 734, 839, 1947
BseNI ACTGG 3 cut(s) 622, 1215, 1324
BseRI GAGGAG 2 cut(s) 77, 104
BseSI GKGCMC 1 cut(s) 510
BseXI GCAGC 5 cut(s) 452, 666, 1268, 1361, 1707
BseYI CCCAGC 2 cut(s) 1547, 1706
Bsh1285I CGRYCG 1 cut(s) 730
BshFI GGCC 5 cut(s) 177, 347, 1298, 1571, 2024
BsiEI CGRYCG 1 cut(s) 730
BsiHKAI GWGCWC 5 cut(s) 332, 510, 826, 1129, 1141
BsiSI CCGG 2 cut(s) 174, 1033
BslFI GGGAC 2 cut(s) 254, 894
BslI CCNNNNNNNGG 1 cut(s) 1039
BsmAI GTCTC 1 cut(s) 1223
BsmFI GGGAC 2 cut(s) 254, 894
BsmI GAATGC 4 cut(s) 275, 703, 1110, 1312
BsnI GGCC 5 cut(s) 177, 347, 1298, 1571, 2024
Bsp119I TTCGAA 3 cut(s) 267, 1686, 2068
Bsp1286I GDGCHC 6 cut(s) 332, 510, 826, 1129, 1141, 1714
Bsp143I GATC 7 cut(s) 70, 459, 519, 990, 1531, 2040, 2130
Bsp1720I GCTNAGC 1 cut(s) 1324
Bsp19I CCATGG 3 cut(s) 463, 1435, 1584
BspACI CCGC 7 cut(s) 63, 593, 652, 686, 726, 1242, 1502
BspANI GGCC 5 cut(s) 177, 347, 1298, 1571, 2024
BspCNI CTCAG 5 cut(s) 457, 576, 733, 838, 1948
BspHI TCATGA 1 cut(s) 1846
BspLI GGNNCC 3 cut(s) 242, 783, 1695
BspPI GGATC 2 cut(s) 65, 454
BspT104I TTCGAA 3 cut(s) 267, 1686, 2068
BsrBI CCGCTC 1 cut(s) 726
BsrDI GCAATG 4 cut(s) 708, 1317, 1335, 2025
BsrFI RCCGGY 2 cut(s) 173, 1032
BsrI ACTGG 3 cut(s) 622, 1215, 1324
BssAI RCCGGY 2 cut(s) 173, 1032
BssECI CCNNGG 8 cut(s) 348, 463, 877, 1047, 1147, 1435, 1453, 1584
BssMI GATC 7 cut(s) 70, 459, 519, 990, 1531, 2040, 2130
BssNI GRCGYC 1 cut(s) 1459
BssT1I CCWWGG 6 cut(s) 463, 1047, 1147, 1435, 1453, 1584
Bst2UI CCWGG 3 cut(s) 878, 1812, 2055
Bst4CI ACNGT 1 cut(s) 808
Bst6I CTCTTC 1 cut(s) 1626
BstACI GRCGYC 1 cut(s) 1459
BstBI TTCGAA 3 cut(s) 267, 1686, 2068
BstC8I GCNNGC 3 cut(s) 206, 652, 1429
BstDEI CTNAG 7 cut(s) 444, 584, 673, 720, 825, 1324, 1956
BstDSI CCRYGG 4 cut(s) 348, 463, 1435, 1584
BstF5I GGATG 7 cut(s) 372, 649, 991, 1526, 1762, 1873, 1981
BstH2I RGCGCY 1 cut(s) 384
BstHHI GCGC 2 cut(s) 224, 383
BstKTI GATC 7 cut(s) 73, 462, 522, 993, 1534, 2043, 2133
BstMAI GTCTC 1 cut(s) 1223
BstMBI GATC 7 cut(s) 70, 459, 519, 990, 1531, 2040, 2130
BstMCI CGRYCG 1 cut(s) 730
BstMWI GCNNNNNNNGC 2 cut(s) 808, 1349
BstNI CCWGG 3 cut(s) 878, 1812, 2055
BstNSI RCATGY 4 cut(s) 473, 529, 1231, 1682
BstSCI CCNGG 3 cut(s) 876, 1810, 2053
BstSLI GKGCMC 1 cut(s) 510
BstV1I GCAGC 5 cut(s) 452, 666, 1268, 1361, 1707
BstV2I GAAGAC 3 cut(s) 256, 879, 1586
BstX2I RGATCY 3 cut(s) 459, 1531, 2130
BstXI CCANNNNNNTGG 2 cut(s) 884, 1294
BstYI RGATCY 3 cut(s) 459, 1531, 2130
BsuRI GGCC 5 cut(s) 177, 347, 1298, 1571, 2024
BtgI CCRYGG 4 cut(s) 348, 463, 1435, 1584
BtrI CACGTC 1 cut(s) 55
BtsCI GGATG 7 cut(s) 372, 649, 991, 1526, 1762, 1873, 1981
BtsI GCAGTG 1 cut(s) 1193
BtsIMutI CAGTG 1 cut(s) 1193
Cac8I GCNNGC 3 cut(s) 206, 652, 1429
CciI TCATGA 1 cut(s) 1846
CfoI GCGC 2 cut(s) 224, 383
Cfr10I RCCGGY 2 cut(s) 173, 1032
Cfr13I GGNCC 2 cut(s) 241, 1570
CseI GACGC 1 cut(s) 1467
Csp6I GTAC 6 cut(s) 79, 795, 925, 1036, 1818, 1961
CspCI CAANNNNNGTGG 2 cut(s) 1420, 1455
CviQI GTAC 6 cut(s) 79, 795, 925, 1036, 1818, 1961
DdeI CTNAG 7 cut(s) 444, 584, 673, 720, 825, 1324, 1956
DpnI GATC 7 cut(s) 72, 461, 521, 992, 1533, 2042, 2132
DpnII GATC 7 cut(s) 70, 459, 519, 990, 1531, 2040, 2130
EaeI YGGCCR 1 cut(s) 2022
Eam1104I CTCTTC 1 cut(s) 1626
EarI CTCTTC 1 cut(s) 1626
Ecl136II GAGCTC 2 cut(s) 330, 1139
Eco130I CCWWGG 6 cut(s) 463, 1047, 1147, 1435, 1453, 1584
Eco24I GRGCYC 3 cut(s) 332, 1141, 1714
Eco32I GATATC 2 cut(s) 1402, 2124
Eco47I GGWCC 1 cut(s) 241
Eco53kI GAGCTC 2 cut(s) 330, 1139
EcoICRI GAGCTC 2 cut(s) 330, 1139
EcoO109I RGGNCCY 2 cut(s) 241, 1570
EcoRII CCWGG 3 cut(s) 876, 1810, 2053
EcoRV GATATC 2 cut(s) 1402, 2124
EcoT14I CCWWGG 6 cut(s) 463, 1047, 1147, 1435, 1453, 1584
EcoT22I ATGCAT 1 cut(s) 1335
EcoT38I GRGCYC 3 cut(s) 332, 1141, 1714
ErhI CCWWGG 6 cut(s) 463, 1047, 1147, 1435, 1453, 1584
FalI AAGNNNNNCTT 2 cut(s) 1031, 1063
FaqI GGGAC 2 cut(s) 254, 894
FauI CCCGC 2 cut(s) 70, 659
FbaI TGATCA 2 cut(s) 519, 2040
FblI GTMKAC 1 cut(s) 1449
Fnu4HI GCNGC 5 cut(s) 441, 655, 1257, 1350, 1721
FokI GGATG 7 cut(s) 379, 656, 998, 1533, 1769, 1860, 1988
FriOI GRGCYC 3 cut(s) 332, 1141, 1714
Fsp4HI GCNGC 5 cut(s) 441, 655, 1257, 1350, 1721
FspBI CTAG 3 cut(s) 954, 1445, 1538
GlaI GCGC 2 cut(s) 223, 382
GluI GCNGC 5 cut(s) 441, 655, 1257, 1350, 1721
GsaI CCCAGC 2 cut(s) 1551, 1710
GsuI CTGGAG 1 cut(s) 1193
HaeII RGCGCY 1 cut(s) 384
HaeIII GGCC 5 cut(s) 177, 347, 1298, 1571, 2024
HapII CCGG 2 cut(s) 174, 1033
HgaI GACGC 1 cut(s) 1467
HhaI GCGC 2 cut(s) 224, 383
Hin1I GRCGYC 1 cut(s) 1459
Hin6I GCGC 2 cut(s) 222, 381
HinP1I GCGC 2 cut(s) 222, 381
HindIII AAGCTT 1 cut(s) 1291
HpaII CCGG 2 cut(s) 174, 1033
HphI GGTGA 4 cut(s) 554, 1491, 1673, 1874
Hpy166II GTNNAC 5 cut(s) 154, 508, 603, 1168, 1450
Hpy188I TCNGA 8 cut(s) 70, 285, 496, 519, 585, 723, 760, 1957
Hpy188III TCNNGA 7 cut(s) 169, 446, 1142, 1210, 1366, 1801, 1847
Hpy8I GTNNAC 5 cut(s) 154, 508, 603, 1168, 1450
HpyAV CCTTC 6 cut(s) 59, 417, 443, 1376, 1981, 1985
HpyCH4III ACNGT 1 cut(s) 808
HpyCH4IV ACGT 2 cut(s) 54, 1072
HpyF10VI GCNNNNNNNGC 2 cut(s) 808, 1349
HpyF3I CTNAG 7 cut(s) 444, 584, 673, 720, 825, 1324, 1956
HpySE526I ACGT 2 cut(s) 54, 1072
Hsp92I GRCGYC 1 cut(s) 1459
HspAI GCGC 2 cut(s) 222, 381
Ksp22I TGATCA 2 cut(s) 519, 2040
Kzo9I GATC 7 cut(s) 70, 459, 519, 990, 1531, 2040, 2130
LmnI GCTCC 2 cut(s) 1136, 1693
Lsp1109I GCAGC 5 cut(s) 452, 666, 1268, 1361, 1707
LweI GCATC 6 cut(s) 141, 487, 580, 637, 673, 1882
MaeI CTAG 3 cut(s) 954, 1445, 1538
MaeII ACGT 2 cut(s) 54, 1072
MaeIII GTNAC 3 cut(s) 834, 1096, 1862
MalI GATC 7 cut(s) 72, 461, 521, 992, 1533, 2042, 2132
MbiI CCGCTC 1 cut(s) 726
MboI GATC 7 cut(s) 70, 459, 519, 990, 1531, 2040, 2130
MfeI CAATTG 1 cut(s) 2209
MflI RGATCY 3 cut(s) 459, 1531, 2130
MhlI GDGCHC 6 cut(s) 332, 510, 826, 1129, 1141, 1714
MlsI TGGCCA 1 cut(s) 2024
MluNI TGGCCA 1 cut(s) 2024
MlyI GAGTC 2 cut(s) 586, 2011
Mox20I TGGCCA 1 cut(s) 2024
Mph1103I ATGCAT 1 cut(s) 1335
MroXI GAANNNNTTC 1 cut(s) 2168
MscI TGGCCA 1 cut(s) 2024
MseI TTAA 3 cut(s) 906, 1064, 1880
MslI CAYNNNNRTG 6 cut(s) 468, 1226, 1409, 1589, 1647, 2010
Msp20I TGGCCA 1 cut(s) 2024
MspI CCGG 2 cut(s) 174, 1033
MspR9I CCNGG 3 cut(s) 878, 1812, 2055
MunI CAATTG 1 cut(s) 2209
Mva1269I GAATGC 4 cut(s) 275, 703, 1110, 1312
MvaI CCWGG 3 cut(s) 878, 1812, 2055
MwoI GCNNNNNNNGC 2 cut(s) 808, 1349
NcoI CCATGG 3 cut(s) 463, 1435, 1584
NdeII GATC 7 cut(s) 70, 459, 519, 990, 1531, 2040, 2130
NlaIV GGNNCC 3 cut(s) 242, 783, 1695
NmuCI GTSAC 3 cut(s) 834, 1096, 1862
NsiI ATGCAT 1 cut(s) 1335
NspI RCATGY 4 cut(s) 473, 529, 1231, 1682
NspV TTCGAA 3 cut(s) 267, 1686, 2068
OliI CACNNNNGTG 1 cut(s) 1409
PagI TCATGA 1 cut(s) 1846
PciI ACATGT 3 cut(s) 525, 1227, 1678
PctI GAATGC 4 cut(s) 275, 703, 1110, 1312
PdmI GAANNNNTTC 1 cut(s) 2168
PfeI GAWTC 8 cut(s) 280, 370, 1159, 1386, 1638, 1797, 2080, 2164
PkrI GCNGC 5 cut(s) 442, 656, 1258, 1351, 1722
PleI GAGTC 2 cut(s) 585, 2010
PpsI GAGTC 2 cut(s) 585, 2010
PpuMI RGGWCCY 1 cut(s) 241
PscI ACATGT 3 cut(s) 525, 1227, 1678
Psp124BI GAGCTC 2 cut(s) 332, 1141
Psp5II RGGWCCY 1 cut(s) 241
Psp6I CCWGG 3 cut(s) 876, 1810, 2053
PspFI CCCAGC 2 cut(s) 1547, 1706
PspGI CCWGG 3 cut(s) 876, 1810, 2053
PspN4I GGNNCC 3 cut(s) 242, 783, 1695
PspPI GGNCC 2 cut(s) 241, 1570
PspPPI RGGWCCY 1 cut(s) 241
PsuI RGATCY 3 cut(s) 459, 1531, 2130
RsaI GTAC 6 cut(s) 80, 796, 926, 1037, 1819, 1962
RsaNI GTAC 6 cut(s) 79, 795, 925, 1036, 1818, 1961
RseI CAYNNNNRTG 6 cut(s) 468, 1226, 1409, 1589, 1647, 2010
SacI GAGCTC 2 cut(s) 332, 1141
SaqAI TTAA 3 cut(s) 906, 1064, 1880
SatI GCNGC 5 cut(s) 441, 655, 1257, 1350, 1721
Sau3AI GATC 7 cut(s) 70, 459, 519, 990, 1531, 2040, 2130
Sau96I GGNCC 2 cut(s) 241, 1570
ScaI AGTACT 2 cut(s) 796, 1819
SchI GAGTC 2 cut(s) 586, 2011
ScrFI CCNGG 3 cut(s) 878, 1812, 2055
SduI GDGCHC 6 cut(s) 332, 510, 826, 1129, 1141, 1714
SfaNI GCATC 6 cut(s) 141, 487, 580, 637, 673, 1882
SfuI TTCGAA 3 cut(s) 267, 1686, 2068
SinI GGWCC 1 cut(s) 241
SmiMI CAYNNNNRTG 6 cut(s) 468, 1226, 1409, 1589, 1647, 2010
SmlI CTYRAG 2 cut(s) 325, 1355
SmoI CTYRAG 2 cut(s) 325, 1355
SsiI CCGC 7 cut(s) 63, 593, 652, 686, 726, 1242, 1502
SspI AATATT 1 cut(s) 1466
SspMI CTAG 3 cut(s) 954, 1445, 1538
SstI GAGCTC 2 cut(s) 332, 1141
StyD4I CCNGG 3 cut(s) 876, 1810, 2053
StyI CCWWGG 6 cut(s) 463, 1047, 1147, 1435, 1453, 1584
TaaI ACNGT 1 cut(s) 808
TaiI ACGT 2 cut(s) 57, 1075
TaqI TCGA 9 cut(s) 213, 267, 313, 332, 1251, 1367, 1686, 2068, 2086
TaqII GACCGA 1 cut(s) 250
TatI WGTACW 3 cut(s) 794, 924, 1817
TfiI GAWTC 8 cut(s) 280, 370, 1159, 1386, 1638, 1797, 2080, 2164
Tru1I TTAA 3 cut(s) 906, 1064, 1880
Tru9I TTAA 3 cut(s) 906, 1064, 1880
TscAI CASTG 1 cut(s) 1193
TseFI GTSAC 3 cut(s) 834, 1096, 1862
TseI GCWGC 5 cut(s) 440, 654, 1256, 1349, 1720
Tsp45I GTSAC 3 cut(s) 834, 1096, 1862
TspDTI ATGAA 9 cut(s) 6, 681, 1151, 1395, 1575, 1592, 1607, 1835, 2187
TspGWI ACGGA 1 cut(s) 66
TspRI CASTG 1 cut(s) 1193
VneI GTGCAC 1 cut(s) 506
VpaK11BI GGWCC 1 cut(s) 241
XapI RAATTY 1 cut(s) 1995
XceI RCATGY 4 cut(s) 473, 529, 1231, 1682
XcmI CCANNNNNNNNNTGG 1 cut(s) 2183
XmiI GTMKAC 1 cut(s) 1449
XmnI GAANNNNTTC 1 cut(s) 2168
XspI CTAG 3 cut(s) 954, 1445, 1538
ZrmI AGTACT 2 cut(s) 796, 1819
Zsp2I ATGCAT 1 cut(s) 1335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.