FvH4_1g07670

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000515
Physical Location & Seq
Reverse (-)
4074015 .. 4075859
1845 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g07670.t1

Sequence Viewer

Length: 804 bp
ATGGCAGAGGCTGCACAGATGCTAGTTTCAGTGAGCCAGAAGCAGAAAGTCCTTGTGACCAACAAACATGGTGAAAAGCTGGTTGGCTTATTACATGAGACTGGTTCAAGGGAGATTGTGATATTGTGCCATGGAGCTAGAGCCTCAAAGGACCATTTTATTATTGAGAACCTTGCTGTTGCGTTGGAAAAGGAAGGAATTAGTTCCTTCCGTTTTGATTTTGCTGGAAATGGAGAGAGTGAAGGCACCTTTCAGTTTGGTCACATGAGGAGAGAAGCTGATGACTTGCATGCTGTAATCCAACACTTCTCCGGATCAAACCATGTAGTAAGTGCAATTTTCGGGCACAGTAAAGGAGCCGGTGATGTCCTCCTCTATGCTTCAAAGTATCATGACATTCCTACGGTTGTTGAAGCTTCTGCACGCTATGACCCAAAGAAAGGCATTGAGAAACTTCTTGGGAAAGACTTTATGGAGAAGATAAAAAAGGATGGATTCATTGATATTGGAAGTGCAAATTTTCGGGTGACTGAGGAAAGCTTGATGGATCGGCTAAGCACTGATATGCATGAATCATGCCTCAAAATTGACAAAGATTGCCGGGTGCTGATAGTCCATGGATCTGCTGATGAGTCCATTCCAGTTGAAGATGCATTTGAGTTTGCCAAGATCATACCTAACCACAAAATACATATTATACAAGGAGCTGATCATTGTTACACGTCGCATCAAGCCGAGTTAGACACAGTTGTTGCGGACTTCATAAAGGCAGCTCTGCAGCAGGACAAATCTACTTCCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

268

Amino Acids

29.47

Weight (kDa)

5.87

Isoelectric Point (pI)

24.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 36 - 154 1.7e-11 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47590 AT3G47590
fragaria_vesca FvH4_1g07650 FvH4_1g07670 FvH4_1g07671 FvH4_1g07680 FvH4_1g07690 FvH4_1g07700 FvH4_1g07700
malus_domestica MD02G1080900.v1.1 MD02G1081000.v1.1 MD02G1081300.v1.1 MD02G1081400.v1.1 MD02G1094000.v1.1 MD15G1208700.v1.1
prunus_persica Prupe.7G208200_v2.0.a1 Prupe.7G208200_v2.0.a1
pyrus_communis pycom02g07440 pycom15g18480
rosa_chinensis RchiOBHm_Chr2g0093511 RchiOBHm_Chr2g0093521 RchiOBHm_Chr2g0093531 RchiOBHm_Chr2g0093541 RchiOBHm_Chr2g0093551 RchiOBHm_Chr2g0093561
rosa_laevigata RLG00000016413 RLG00000016414 RLG00000016415 RLG00000016416 RLG00000016417
rosa_multiflora Rmu_sc0004423.1_g000003 Rmu_sc0004423.1_g000004 Rmu_sc0004423.1_g000005 Rmu_sc0004423.1_g000006 Rmu_sc0006964.1_g000003 Rmu_sc0039672.1_g000001 Rmu_sc0039672.1_g000003
rosa_roxburghii Rroxscaffold_2G00147990 Rroxscaffold_2G00148000 Rroxscaffold_2G00148010 Rroxscaffold_2G00148020 Rroxscaffold_4G00313540
rosa_rugosa Rorug01G0140600.1 Rorug01G0140700.1 Rorug02G0037000 Rorug02G0037100 Rorug02G0037200 Rorug02G0037300 Rorug02G0037400 Rorug02G0037500 Rorug02G0037600
rosa_samantha Rh2AG083400 Rh2AG083500 Rh2AG083700 Rh2AG083800 Rh2AG083900 Rh2AG084000 Rh2BG084200 Rh2BG084300 Rh2BG084400 Rh2BG084500 Rh2BG084600 Rh2CG086300 Rh2CG086400 Rh2CG086500 Rh2CG086700 Rh2CG086800 Rh2CG086900 Rh2DG082200 Rh2DG082300 Rh2DG082400 Rh2DG082500 Rh2DG082600 Rh2DG082700
rosa_wichuraiana Rw1G013200 Rw2G006360 Rw2G006370 Rw2G006380 Rw2G006390 Rw2G006400 Rw2G007130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 245
AccIII TCCGGA 1 cut(s) 311
AciI CCGC 1 cut(s) 755
AclWI GGATC 3 cut(s) 322, 555, 628
AcsI RAATTY 1 cut(s) 517
AfiI CCNNNNNNNGG 1 cut(s) 440
AflIII ACRYGT 1 cut(s) 720
AgsI TTSAA 4 cut(s) 108, 384, 413, 647
AjiI CACGTC 1 cut(s) 723
AjuI GAANNNNNNNTTGG 2 cut(s) 66, 98
AluBI AGCT 7 cut(s) 79, 137, 278, 416, 540, 707, 773
AluI AGCT 7 cut(s) 79, 137, 278, 416, 540, 707, 773
Alw26I GTCTC 1 cut(s) 92
AlwI GGATC 3 cut(s) 322, 555, 628
AlwNI CAGNNNCTG 1 cut(s) 11
Aor13HI TCCGGA 1 cut(s) 311
ApeKI GCWGC 3 cut(s) 11, 770, 778
ApoI RAATTY 1 cut(s) 517
Asp700I GAANNNNTTC 1 cut(s) 202
AspS9I GGNCC 1 cut(s) 151
AsuC2I CCSGG 1 cut(s) 602
AsuHPI GGTGA 3 cut(s) 83, 374, 538
AvaII GGWCC 1 cut(s) 151
BaeGI GKGCMC 1 cut(s) 348
BanI GGYRCC 1 cut(s) 245
BbvI GCAGC 2 cut(s) 782, 790
BccI CCATC 2 cut(s) 485, 538
BclI TGATCA 1 cut(s) 709
BcnI CCSGG 1 cut(s) 602
BcoDI GTCTC 1 cut(s) 92
BfaI CTAG 3 cut(s) 23, 138, 802
BfmI CTRYAG 1 cut(s) 776
BisI GCNGC 3 cut(s) 12, 771, 779
BlpI GCTNAGC 1 cut(s) 554
BlsI GCNGC 3 cut(s) 13, 772, 780
Bme1390I CCNGG 1 cut(s) 602
Bme18I GGWCC 1 cut(s) 151
BmgBI CACGTC 1 cut(s) 723
BmgT120I GGNCC 1 cut(s) 151
BmiI GGNNCC 2 cut(s) 247, 358
BmrFI CCNGG 1 cut(s) 602
BmsI GCATC 3 cut(s) 9, 640, 736
Bpu1102I GCTNAGC 1 cut(s) 554
BpuMI CCSGG 1 cut(s) 602
BsaJI CCNNGG 2 cut(s) 130, 616
BsaWI WCCGGW 1 cut(s) 311
Bsc4I CCNNNNNNNGG 1 cut(s) 440
Bse118I RCCGGY 1 cut(s) 359
Bse1I ACTGG 2 cut(s) 106, 641
BseAI TCCGGA 1 cut(s) 311
BseDI CCNNGG 2 cut(s) 130, 616
BseGI GGATG 1 cut(s) 496
BseLI CCNNNNNNNGG 1 cut(s) 440
BseMII CTCAG 1 cut(s) 522
BseNI ACTGG 2 cut(s) 106, 641
BseRI GAGGAG 2 cut(s) 283, 362
BseSI GKGCMC 1 cut(s) 348
BseXI GCAGC 2 cut(s) 782, 790
BsgI GTGCAG 1 cut(s) 405
BshNI GGYRCC 1 cut(s) 245
BsiSI CCGG 3 cut(s) 312, 360, 601
BslI CCNNNNNNNGG 1 cut(s) 440
BsmAI GTCTC 1 cut(s) 92
Bsp1286I GDGCHC 1 cut(s) 348
Bsp13I TCCGGA 1 cut(s) 311
Bsp143I GATC 5 cut(s) 314, 547, 620, 669, 709
Bsp1720I GCTNAGC 1 cut(s) 554
Bsp19I CCATGG 2 cut(s) 130, 616
BspACI CCGC 1 cut(s) 755
BspCNI CTCAG 1 cut(s) 523
BspEI TCCGGA 1 cut(s) 311
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 2 cut(s) 247, 358
BspMAI CTGCAG 1 cut(s) 780
BspPI GGATC 3 cut(s) 322, 555, 628
BspT107I GGYRCC 1 cut(s) 245
BsrFI RCCGGY 1 cut(s) 359
BsrI ACTGG 2 cut(s) 106, 641
BssAI RCCGGY 1 cut(s) 359
BssECI CCNNGG 2 cut(s) 130, 616
BssMI GATC 5 cut(s) 314, 547, 620, 669, 709
BssT1I CCWWGG 2 cut(s) 130, 616
Bst4CI ACNGT 3 cut(s) 350, 406, 748
BstAPI GCANNNNNTGC 1 cut(s) 11
BstC8I GCNNGC 2 cut(s) 291, 424
BstDEI CTNAG 2 cut(s) 531, 554
BstDSI CCRYGG 2 cut(s) 130, 616
BstF5I GGATG 1 cut(s) 496
BstKTI GATC 5 cut(s) 317, 550, 623, 672, 712
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 5 cut(s) 314, 547, 620, 669, 709
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNSI RCATGY 1 cut(s) 293
BstSCI CCNGG 1 cut(s) 600
BstSFI CTRYAG 1 cut(s) 776
BstSLI GKGCMC 1 cut(s) 348
BstV1I GCAGC 2 cut(s) 782, 790
BstX2I RGATCY 1 cut(s) 620
BstYI RGATCY 1 cut(s) 620
BtgI CCRYGG 2 cut(s) 130, 616
BtrI CACGTC 1 cut(s) 723
BtsCI GGATG 1 cut(s) 496
BtsIMutI CAGTG 2 cut(s) 36, 558
Cac8I GCNNGC 2 cut(s) 291, 424
CaiI CAGNNNCTG 1 cut(s) 11
CciI TCATGA 1 cut(s) 391
Cfr10I RCCGGY 1 cut(s) 359
Cfr13I GGNCC 1 cut(s) 151
DdeI CTNAG 2 cut(s) 531, 554
DpnI GATC 5 cut(s) 316, 549, 622, 671, 711
DpnII GATC 5 cut(s) 314, 547, 620, 669, 709
Eco130I CCWWGG 2 cut(s) 130, 616
Eco47I GGWCC 1 cut(s) 151
EcoT14I CCWWGG 2 cut(s) 130, 616
EcoT22I ATGCAT 2 cut(s) 570, 655
ErhI CCWWGG 2 cut(s) 130, 616
FbaI TGATCA 1 cut(s) 709
Fnu4HI GCNGC 3 cut(s) 12, 771, 779
FokI GGATG 1 cut(s) 503
Fsp4HI GCNGC 3 cut(s) 12, 771, 779
FspBI CTAG 3 cut(s) 23, 138, 802
GluI GCNGC 3 cut(s) 12, 771, 779
HapII CCGG 3 cut(s) 312, 360, 601
HindIII AAGCTT 2 cut(s) 414, 538
HinfI GANTC 3 cut(s) 495, 572, 632
HpaII CCGG 3 cut(s) 312, 360, 601
HphI GGTGA 3 cut(s) 83, 374, 538
Hpy188III TCNNGA 2 cut(s) 312, 392
Hpy99I CGWCG 1 cut(s) 727
HpyAV CCTTC 3 cut(s) 188, 217, 236
HpyCH4III ACNGT 3 cut(s) 350, 406, 748
HpyCH4IV ACGT 1 cut(s) 722
HpyCH4V TGCA 8 cut(s) 14, 289, 335, 422, 515, 568, 653, 778
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 2 cut(s) 531, 554
HpySE526I ACGT 1 cut(s) 722
Kpn2I TCCGGA 1 cut(s) 311
Ksp22I TGATCA 1 cut(s) 709
Kzo9I GATC 5 cut(s) 314, 547, 620, 669, 709
LmnI GCTCC 3 cut(s) 134, 356, 704
LpnPI CCDG 9 cut(s) 50, 65, 87, 210, 325, 373, 614, 654, 767
Lsp1109I GCAGC 2 cut(s) 782, 790
LweI GCATC 3 cut(s) 9, 640, 736
MaeI CTAG 3 cut(s) 23, 138, 802
MaeII ACGT 1 cut(s) 722
MaeIII GTNAC 4 cut(s) 55, 260, 526, 716
MalI GATC 5 cut(s) 316, 549, 622, 671, 711
MboI GATC 5 cut(s) 314, 547, 620, 669, 709
MboII GAAGA 2 cut(s) 490, 659
MflI RGATCY 1 cut(s) 620
MhlI GDGCHC 1 cut(s) 348
MluCI AATT 4 cut(s) 198, 336, 517, 585
MlyI GAGTC 1 cut(s) 641
MmeI TCCRAC 2 cut(s) 165, 325
MnlI CCTC 6 cut(s) 154, 261, 380, 383, 526, 590
Mph1103I ATGCAT 2 cut(s) 570, 655
MroI TCCGGA 1 cut(s) 311
MroXI GAANNNNTTC 1 cut(s) 202
MslI CAYNNNNRTG 1 cut(s) 563
MspI CCGG 3 cut(s) 312, 360, 601
MspR9I CCNGG 1 cut(s) 602
MwoI GCNNNNNNNGC 1 cut(s) 11
NciI CCSGG 1 cut(s) 602
NcoI CCATGG 2 cut(s) 130, 616
NdeII GATC 5 cut(s) 314, 547, 620, 669, 709
NlaIV GGNNCC 2 cut(s) 247, 358
NmeAIII GCCGAG 1 cut(s) 760
NmuCI GTSAC 3 cut(s) 55, 260, 526
NsiI ATGCAT 2 cut(s) 570, 655
NspI RCATGY 1 cut(s) 293
PaeI GCATGC 1 cut(s) 293
PagI TCATGA 1 cut(s) 391
PdmI GAANNNNTTC 1 cut(s) 202
PfeI GAWTC 2 cut(s) 495, 572
PkrI GCNGC 3 cut(s) 13, 772, 780
PleI GAGTC 1 cut(s) 640
PpsI GAGTC 1 cut(s) 640
PspN4I GGNNCC 2 cut(s) 247, 358
PspPI GGNCC 1 cut(s) 151
PstI CTGCAG 1 cut(s) 780
PstNI CAGNNNCTG 1 cut(s) 11
PsuI RGATCY 1 cut(s) 620
RseI CAYNNNNRTG 1 cut(s) 563
SatI GCNGC 3 cut(s) 12, 771, 779
Sau3AI GATC 5 cut(s) 314, 547, 620, 669, 709
Sau96I GGNCC 1 cut(s) 151
SchI GAGTC 1 cut(s) 641
ScrFI CCNGG 1 cut(s) 602
SduI GDGCHC 1 cut(s) 348
SfaNI GCATC 3 cut(s) 9, 640, 736
SfcI CTRYAG 1 cut(s) 776
SinI GGWCC 1 cut(s) 151
SmiMI CAYNNNNRTG 1 cut(s) 563
SphI GCATGC 1 cut(s) 293
Sse9I AATT 4 cut(s) 198, 336, 517, 585
SsiI CCGC 1 cut(s) 755
SspMI CTAG 3 cut(s) 23, 138, 802
StyD4I CCNGG 1 cut(s) 600
StyI CCWWGG 2 cut(s) 130, 616
TaaI ACNGT 3 cut(s) 350, 406, 748
TaiI ACGT 1 cut(s) 725
TasI AATT 4 cut(s) 198, 336, 517, 585
TfiI GAWTC 2 cut(s) 495, 572
TscAI CASTG 2 cut(s) 36, 565
TseFI GTSAC 3 cut(s) 55, 260, 526
TseI GCWGC 3 cut(s) 11, 770, 778
Tsp45I GTSAC 3 cut(s) 55, 260, 526
TspDTI ATGAA 3 cut(s) 487, 585, 751
TspGWI ACGGA 1 cut(s) 200
TspRI CASTG 2 cut(s) 36, 565
VpaK11BI GGWCC 1 cut(s) 151
XapI RAATTY 1 cut(s) 517
XceI RCATGY 1 cut(s) 293
XmnI GAANNNNTTC 1 cut(s) 202
XspI CTAG 3 cut(s) 23, 138, 802
Zsp2I ATGCAT 2 cut(s) 570, 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.