FvH4_1g07700

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000515
Physical Location & Seq
Reverse (-)
4084693 .. 4087351
2659 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g07700.t2

Sequence Viewer

Length: 900 bp
ATGCTGCTCTCAAATTCTTATTCTTTAACTTCCCCAAAGTTCCCATCGCCAAAACTCACTCTTCACCACAATCGCAGCCCAAAACGAATCGTCAGAATGTCCCAGACTTCCGAAAACCCAGTGAAGCAGCAACTGAAAATCATCGTACCCAACAAACACGGTGAGAAACTTGTTGGCTTATTACATGACACAGGTTCTGGGGAGATTGTAATCTTATGTCACGGTTTTCGATCCTCCAAGGAAACGAAGAGTATTGCCAACATTGCTGTTGCATTGGAGAATGAAGGAATTAGTTCGTTTCGTTTTGACTTTGCTGGAAATGGGGAAAGTGAAGGTATATTTCAGTATGGTCACTATCGTAGAGAGGCCGACGACTTGCATGCTGTAGTCCAACACTTCTCTGGGGAAAATCGTGTTCCAAGTGCAATTCTTGGGCACAGTAAAGGAGGCGATGTTGTACTCCTATATGCTTCCAAGTATCATGACATTCCTACTGTTGTCAATGTTTCTGGGCGTTATGATATGAAGAGAGGCGTTAAAGAACGGTTTGGAGAAGACTTTATGGAAACAATCAAGAAGGAAGGATTCTTTGATGTTCAGGATAAGAGAGGAAGTTATCGTGTGACCGAGGAAAGTTTGATGGATCGCCTAAGTACAGATATGCATGAAGCGTGCCTTCAGATTGACAAAGATTGTCGGGTGTTAACTGTCCACGGGTCTGCTGATGAAGTCATTGCTATTGAAGATGCGTTAGAGTTTGCGAAGATTATACCTAACCACAAATTACATATCGTAGAGGGAGCTAACCATAACTACAGCTCACATCAAGCCGAGTTAGCATCAGTTGTTGTGGACTTCATAAAGACATCACTGCAGCAGGACAAGCAGCCTACTTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

300

Amino Acids

33.43

Weight (kDa)

6.46

Isoelectric Point (pI)

47.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 69 - 176 1.5e-13 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 69 - 171 3e-07 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47590 AT3G47590
fragaria_vesca FvH4_1g07650 FvH4_1g07670 FvH4_1g07671 FvH4_1g07680 FvH4_1g07690 FvH4_1g07700 FvH4_1g07700
malus_domestica MD02G1080900.v1.1 MD02G1081000.v1.1 MD02G1081300.v1.1 MD02G1081400.v1.1 MD02G1094000.v1.1 MD15G1208700.v1.1
prunus_persica Prupe.7G208200_v2.0.a1 Prupe.7G208200_v2.0.a1
pyrus_communis pycom02g07440 pycom15g18480
rosa_chinensis RchiOBHm_Chr2g0093511 RchiOBHm_Chr2g0093521 RchiOBHm_Chr2g0093531 RchiOBHm_Chr2g0093541 RchiOBHm_Chr2g0093551 RchiOBHm_Chr2g0093561
rosa_laevigata RLG00000016413 RLG00000016414 RLG00000016415 RLG00000016416 RLG00000016417
rosa_multiflora Rmu_sc0004423.1_g000003 Rmu_sc0004423.1_g000004 Rmu_sc0004423.1_g000005 Rmu_sc0004423.1_g000006 Rmu_sc0006964.1_g000003 Rmu_sc0039672.1_g000001 Rmu_sc0039672.1_g000003
rosa_roxburghii Rroxscaffold_2G00147990 Rroxscaffold_2G00148000 Rroxscaffold_2G00148010 Rroxscaffold_2G00148020 Rroxscaffold_4G00313540
rosa_rugosa Rorug01G0140600.1 Rorug01G0140700.1 Rorug02G0037000 Rorug02G0037100 Rorug02G0037200 Rorug02G0037300 Rorug02G0037400 Rorug02G0037500 Rorug02G0037600
rosa_samantha Rh2AG083400 Rh2AG083500 Rh2AG083700 Rh2AG083800 Rh2AG083900 Rh2AG084000 Rh2BG084200 Rh2BG084300 Rh2BG084400 Rh2BG084500 Rh2BG084600 Rh2CG086300 Rh2CG086400 Rh2CG086500 Rh2CG086700 Rh2CG086800 Rh2CG086900 Rh2DG082200 Rh2DG082300 Rh2DG082400 Rh2DG082500 Rh2DG082600 Rh2DG082700
rosa_wichuraiana Rw1G013200 Rw2G006360 Rw2G006370 Rw2G006380 Rw2G006390 Rw2G006400 Rw2G007130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 225, 651
AcsI RAATTY 1 cut(s) 13
AcuI CTGAAG 1 cut(s) 662
AfaI GTAC 3 cut(s) 147, 459, 655
AgsI TTSAA 1 cut(s) 743
AluBI AGCT 2 cut(s) 803, 819
AluI AGCT 2 cut(s) 803, 819
AlwI GGATC 2 cut(s) 225, 651
AlwNI CAGNNNCTG 2 cut(s) 133, 197
AoxI GGCC 1 cut(s) 366
ApeKI GCWGC 5 cut(s) 4, 75, 127, 874, 886
ApoI RAATTY 1 cut(s) 13
Asp700I GAANNNNTTC 1 cut(s) 292
AsuHPI GGTGA 2 cut(s) 56, 173
BaeGI GKGCMC 1 cut(s) 438
BbsI GAAGAC 1 cut(s) 561
BbvI GCAGC 3 cut(s) 87, 139, 886
BccI CCATC 2 cut(s) 52, 634
BcgI CGANNNNNNTGC 2 cut(s) 362, 396
BfaI CTAG 1 cut(s) 898
BfmI CTRYAG 3 cut(s) 384, 814, 872
BisI GCNGC 5 cut(s) 5, 76, 128, 875, 887
BlsI GCNGC 5 cut(s) 6, 77, 129, 876, 888
BmrI ACTGGG 1 cut(s) 113
BmsI GCATC 2 cut(s) 736, 848
BmuI ACTGGG 1 cut(s) 113
BpiI GAAGAC 1 cut(s) 561
BsaBI GATNNNNATC 1 cut(s) 209
BsaJI CCNNGG 3 cut(s) 237, 627, 712
BsaXI ACNNNNNCTCC 2 cut(s) 438, 468
Bse1I ACTGG 1 cut(s) 119
Bse3DI GCAATG 2 cut(s) 261, 732
Bse8I GATNNNNATC 1 cut(s) 209
BseDI CCNNGG 3 cut(s) 237, 627, 712
BseJI GATNNNNATC 1 cut(s) 209
BseMI GCAATG 2 cut(s) 261, 732
BseNI ACTGG 1 cut(s) 119
BseSI GKGCMC 1 cut(s) 438
BseXI GCAGC 3 cut(s) 87, 139, 886
BshFI GGCC 1 cut(s) 368
BslFI GGGAC 1 cut(s) 85
BsmFI GGGAC 1 cut(s) 85
BsnI GGCC 1 cut(s) 368
Bsp1286I GDGCHC 1 cut(s) 438
Bsp143I GATC 2 cut(s) 230, 643
BspANI GGCC 1 cut(s) 368
BspHI TCATGA 1 cut(s) 481
BspMAI CTGCAG 1 cut(s) 876
BspPI GGATC 2 cut(s) 225, 651
BsrDI GCAATG 2 cut(s) 261, 732
BsrI ACTGG 1 cut(s) 119
BssECI CCNNGG 3 cut(s) 237, 627, 712
BssMI GATC 2 cut(s) 230, 643
BssT1I CCWWGG 1 cut(s) 237
Bst4CI ACNGT 6 cut(s) 161, 224, 440, 496, 546, 709
Bst6I CTCTTC 3 cut(s) 66, 242, 521
BstC8I GCNNGC 2 cut(s) 381, 673
BstDEI CTNAG 1 cut(s) 650
BstDSI CCRYGG 1 cut(s) 712
BstKTI GATC 2 cut(s) 233, 646
BstMBI GATC 2 cut(s) 230, 643
BstMWI GCNNNNNNNGC 3 cut(s) 263, 836, 883
BstNSI RCATGY 1 cut(s) 383
BstSFI CTRYAG 3 cut(s) 384, 814, 872
BstSLI GKGCMC 1 cut(s) 438
BstV1I GCAGC 3 cut(s) 87, 139, 886
BstV2I GAAGAC 1 cut(s) 561
BsuRI GGCC 1 cut(s) 368
BtgI CCRYGG 1 cut(s) 712
BtgZI GCGATG 2 cut(s) 30, 465
BtsI GCAGTG 1 cut(s) 869
BtsIMutI CAGTG 2 cut(s) 126, 869
Cac8I GCNNGC 2 cut(s) 381, 673
CaiI CAGNNNCTG 2 cut(s) 133, 197
CciI TCATGA 1 cut(s) 481
Csp6I GTAC 3 cut(s) 146, 458, 654
CviAII CATG 4 cut(s) 185, 380, 482, 665
CviJI RGCY 7 cut(s) 78, 177, 368, 803, 819, 830, 889
CviKI_1 RGCY 7 cut(s) 78, 177, 368, 803, 819, 830, 889
CviQI GTAC 3 cut(s) 146, 458, 654
DdeI CTNAG 1 cut(s) 650
DpnI GATC 2 cut(s) 232, 645
DpnII GATC 2 cut(s) 230, 643
Eam1104I CTCTTC 3 cut(s) 66, 242, 521
EarI CTCTTC 3 cut(s) 66, 242, 521
Eco130I CCWWGG 1 cut(s) 237
Eco57I CTGAAG 1 cut(s) 662
EcoT14I CCWWGG 1 cut(s) 237
EcoT22I ATGCAT 1 cut(s) 666
ErhI CCWWGG 1 cut(s) 237
FaeI CATG 4 cut(s) 188, 383, 485, 668
FalI AAGNNNNNCTT 2 cut(s) 660, 692
FaqI GGGAC 1 cut(s) 85
FatI CATG 4 cut(s) 184, 379, 481, 664
Fnu4HI GCNGC 5 cut(s) 5, 76, 128, 875, 887
Fsp4HI GCNGC 5 cut(s) 5, 76, 128, 875, 887
FspBI CTAG 1 cut(s) 898
GluI GCNGC 5 cut(s) 5, 76, 128, 875, 887
HaeIII GGCC 1 cut(s) 368
Hin1II CATG 4 cut(s) 188, 383, 485, 668
HincII GTYRAC 1 cut(s) 705
HindII GTYRAC 1 cut(s) 705
HinfI GANTC 2 cut(s) 87, 585
HpaI GTTAAC 1 cut(s) 705
HphI GGTGA 2 cut(s) 56, 173
Hpy166II GTNNAC 3 cut(s) 705, 712, 853
Hpy188I TCNGA 3 cut(s) 95, 112, 681
Hpy188III TCNNGA 3 cut(s) 482, 574, 599
Hpy8I GTNNAC 3 cut(s) 705, 712, 853
Hpy99I CGWCG 1 cut(s) 374
HpyAV CCTTC 5 cut(s) 278, 326, 571, 575, 686
HpyCH4III ACNGT 6 cut(s) 161, 224, 440, 496, 546, 709
HpyCH4V TGCA 5 cut(s) 272, 379, 425, 664, 874
HpyF10VI GCNNNNNNNGC 3 cut(s) 263, 836, 883
HpyF3I CTNAG 1 cut(s) 650
Hsp92II CATG 4 cut(s) 188, 383, 485, 668
KspAI GTTAAC 1 cut(s) 705
Kzo9I GATC 2 cut(s) 230, 643
LmnI GCTCC 1 cut(s) 800
LpnPI CCDG 9 cut(s) 116, 132, 177, 183, 300, 387, 495, 584, 863
Lsp1109I GCAGC 3 cut(s) 87, 139, 886
LweI GCATC 2 cut(s) 736, 848
MaeI CTAG 1 cut(s) 898
MaeIII GTNAC 3 cut(s) 218, 350, 622
MalI GATC 2 cut(s) 232, 645
MboI GATC 2 cut(s) 230, 643
MboII GAAGA 6 cut(s) 53, 259, 538, 566, 755, 775
MhlI GDGCHC 1 cut(s) 438
MluCI AATT 4 cut(s) 13, 288, 426, 782
MmeI TCCRAC 1 cut(s) 415
MnlI CCTC 7 cut(s) 244, 358, 440, 524, 602, 622, 790
Mph1103I ATGCAT 1 cut(s) 666
MroXI GAANNNNTTC 1 cut(s) 292
MseI TTAA 3 cut(s) 26, 537, 704
MwoI GCNNNNNNNGC 3 cut(s) 263, 836, 883
NdeII GATC 2 cut(s) 230, 643
NlaIII CATG 4 cut(s) 188, 383, 485, 668
NmeAIII GCCGAG 1 cut(s) 856
NmuCI GTSAC 3 cut(s) 218, 350, 622
NsiI ATGCAT 1 cut(s) 666
NspI RCATGY 1 cut(s) 383
PaeI GCATGC 1 cut(s) 383
PagI TCATGA 1 cut(s) 481
PdmI GAANNNNTTC 1 cut(s) 292
PfeI GAWTC 2 cut(s) 87, 585
PkrI GCNGC 5 cut(s) 6, 77, 129, 876, 888
PstI CTGCAG 1 cut(s) 876
PstNI CAGNNNCTG 2 cut(s) 133, 197
RsaI GTAC 3 cut(s) 147, 459, 655
RsaNI GTAC 3 cut(s) 146, 458, 654
SaqAI TTAA 3 cut(s) 26, 537, 704
SatI GCNGC 5 cut(s) 5, 76, 128, 875, 887
Sau3AI GATC 2 cut(s) 230, 643
SduI GDGCHC 1 cut(s) 438
SetI ASST 5 cut(s) 196, 337, 775, 805, 821
SfaNI GCATC 2 cut(s) 736, 848
SfcI CTRYAG 3 cut(s) 384, 814, 872
SphI GCATGC 1 cut(s) 383
Sse9I AATT 4 cut(s) 13, 288, 426, 782
SspMI CTAG 1 cut(s) 898
StyI CCWWGG 1 cut(s) 237
TaaI ACNGT 6 cut(s) 161, 224, 440, 496, 546, 709
TaqI TCGA 1 cut(s) 229
TaqII GACCGA 1 cut(s) 641
TasI AATT 4 cut(s) 13, 288, 426, 782
TatI WGTACW 2 cut(s) 457, 653
TfiI GAWTC 2 cut(s) 87, 585
Tru1I TTAA 3 cut(s) 26, 537, 704
Tru9I TTAA 3 cut(s) 26, 537, 704
TscAI CASTG 2 cut(s) 126, 876
TseFI GTSAC 3 cut(s) 218, 350, 622
TseI GCWGC 5 cut(s) 4, 75, 127, 874, 886
Tsp45I GTSAC 3 cut(s) 218, 350, 622
TspDTI ATGAA 5 cut(s) 297, 539, 681, 741, 847
TspRI CASTG 2 cut(s) 126, 876
XapI RAATTY 1 cut(s) 13
XceI RCATGY 1 cut(s) 383
XcmI CCANNNNNNNNNTGG 1 cut(s) 398
XmnI GAANNNNTTC 1 cut(s) 292
XspI CTAG 1 cut(s) 898
Zsp2I ATGCAT 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.