MD02G1094000.v1.1

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000515
Physical Location & Seq
Reverse (-)
7472642 .. 7475684
3043 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1094000.v1.1.491

Sequence Viewer

Length: 939 bp
ATGAAGAATCAGGGTCCAACTGGTAAAAAGGAAACTGTTGTGGACCAAAACCTGAAAACGCGACTGGACTGGTCCGATACCCTCCCAGTTCCAAACTCACAGGAGCTGTGTAGATCGCATCGCAGTGCAACGAGCAGCAAGCAAAAGTTTATGGTGGGTCAGCAACAGAAAATCATCGTACCCAACAAACACGGTGAAAAGCTTGTGGGATTGTTACACGAAACCGGTTCCGCGGACCTTGTAATCTTATGTCATGGTTTTCGAGACACCAAGGAAAACTATATTATGGTGAACCTTGCTGTTGCACTGGAAAATGAAGGGATCAGTTCCTTCCGTTTTGACTTTGCCGGAAATGGGGAAAGTGAAGGCACCTTTCAGTTTGGTAACTATCGGAGAGAGGCGGATGACTTGCACTCTGTGGTCAAGTACTTCTCTGGGGCAAAACGTGCACCCAGTGCAATTGTTGGACACAGTAAAGGAGGCGATGCTGTGCTCCTGTATGCTTCAACCTATCATGACATTCGTACGGTTGTCAATGTTTCTGGACGTTATGATCTGAAGAAAGGCATCGAAGAACGCTTGGGGAAAGACATTATGGAAGTAATCAAGAAGGAAGGGTTCATTGATGTTAAGAATAAGTCAGGAGATGGTAGTTATCGTGTGACCGAGGAGAGTTTGATGGATCGCCTAAGTACTGATATGCACCAATCATGCCTTCAGATTGACAATGAATGCCGGGTGGTGACAGTCCATGGAACTGCCGACGAGGTCATCCCTGTTGAAGATGCATTAGAGTTTGCCAAGATAATACCTAACCACAAATTACATCTTATAGAAGGGGCTAATCATTCGTACACCTCGCATCAAGCCGAGTTAGGATCGCTCGTCGTGGACTTCATTAAGGCAGCCCTGCAGCAGGACAAGGCTACTTCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

313

Amino Acids

34.59

Weight (kDa)

6.23

Isoelectric Point (pI)

33.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 80 - 203 3.8e-13 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 80 - 182 6e-06 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47590 AT3G47590
fragaria_vesca FvH4_1g07650 FvH4_1g07670 FvH4_1g07671 FvH4_1g07680 FvH4_1g07690 FvH4_1g07700 FvH4_1g07700
malus_domestica MD02G1080900.v1.1 MD02G1081000.v1.1 MD02G1081300.v1.1 MD02G1081400.v1.1 MD02G1094000.v1.1 MD15G1208700.v1.1
prunus_persica Prupe.7G208200_v2.0.a1 Prupe.7G208200_v2.0.a1
pyrus_communis pycom02g07440 pycom15g18480
rosa_chinensis RchiOBHm_Chr2g0093511 RchiOBHm_Chr2g0093521 RchiOBHm_Chr2g0093531 RchiOBHm_Chr2g0093541 RchiOBHm_Chr2g0093551 RchiOBHm_Chr2g0093561
rosa_laevigata RLG00000016413 RLG00000016414 RLG00000016415 RLG00000016416 RLG00000016417
rosa_multiflora Rmu_sc0004423.1_g000003 Rmu_sc0004423.1_g000004 Rmu_sc0004423.1_g000005 Rmu_sc0004423.1_g000006 Rmu_sc0006964.1_g000003 Rmu_sc0039672.1_g000001 Rmu_sc0039672.1_g000003
rosa_roxburghii Rroxscaffold_2G00147990 Rroxscaffold_2G00148000 Rroxscaffold_2G00148010 Rroxscaffold_2G00148020 Rroxscaffold_4G00313540
rosa_rugosa Rorug01G0140600.1 Rorug01G0140700.1 Rorug02G0037000 Rorug02G0037100 Rorug02G0037200 Rorug02G0037300 Rorug02G0037400 Rorug02G0037500 Rorug02G0037600
rosa_samantha Rh2AG083400 Rh2AG083500 Rh2AG083700 Rh2AG083800 Rh2AG083900 Rh2AG084000 Rh2BG084200 Rh2BG084300 Rh2BG084400 Rh2BG084500 Rh2BG084600 Rh2CG086300 Rh2CG086400 Rh2CG086500 Rh2CG086700 Rh2CG086800 Rh2CG086900 Rh2DG082200 Rh2DG082300 Rh2DG082400 Rh2DG082500 Rh2DG082600 Rh2DG082700
rosa_wichuraiana Rw1G013200 Rw2G006360 Rw2G006370 Rw2G006380 Rw2G006390 Rw2G006400 Rw2G007130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 368
AccII CGCG 2 cut(s) 61, 233
AciI CCGC 3 cut(s) 231, 233, 401
AclWI GGATC 3 cut(s) 329, 690, 886
AcuI CTGAAG 2 cut(s) 578, 701
AdeI CACNNNGTG 2 cut(s) 418, 455
AfaI GTAC 5 cut(s) 180, 428, 526, 694, 854
AfiI CCNNNNNNNGG 2 cut(s) 354, 916
AgeI ACCGGT 1 cut(s) 224
AgsI TTSAA 3 cut(s) 507, 782, 933
AjuI GAANNNNNNNTTGG 2 cut(s) 699, 731
AluBI AGCT 2 cut(s) 106, 202
AluI AGCT 2 cut(s) 106, 202
Alw21I GWGCWC 2 cut(s) 451, 495
Alw26I GTCTC 1 cut(s) 258
Alw44I GTGCAC 1 cut(s) 447
AlwI GGATC 3 cut(s) 329, 690, 886
AlwNI CAGNNNCTG 1 cut(s) 106
ApaLI GTGCAC 1 cut(s) 447
ApeKI GCWGC 3 cut(s) 135, 905, 913
AsiGI ACCGGT 1 cut(s) 224
AspS9I GGNCC 4 cut(s) 14, 43, 72, 235
AsuC2I CCSGG 1 cut(s) 737
AsuHPI GGTGA 3 cut(s) 206, 301, 754
AvaII GGWCC 4 cut(s) 14, 43, 72, 235
BaeGI GKGCMC 1 cut(s) 451
BanI GGYRCC 1 cut(s) 368
Bbv12I GWGCWC 2 cut(s) 451, 495
BbvI GCAGC 3 cut(s) 147, 917, 925
BccI CCATC 2 cut(s) 641, 673
BcnI CCSGG 1 cut(s) 737
BcoDI GTCTC 1 cut(s) 258
BfaI CTAG 1 cut(s) 937
BfmI CTRYAG 1 cut(s) 911
BisI GCNGC 3 cut(s) 136, 906, 914
BlsI GCNGC 3 cut(s) 137, 907, 915
BmcAI AGTACT 2 cut(s) 428, 694
Bme1390I CCNGG 1 cut(s) 737
Bme18I GGWCC 4 cut(s) 14, 43, 72, 235
BmgT120I GGNCC 4 cut(s) 14, 43, 72, 235
BmiI GGNNCC 3 cut(s) 15, 229, 370
BmrFI CCNGG 1 cut(s) 737
BmrI ACTGGG 2 cut(s) 80, 447
BmsI GCATC 5 cut(s) 127, 475, 576, 775, 871
BmuI ACTGGG 2 cut(s) 80, 447
BpuMI CCSGG 1 cut(s) 737
BsaJI CCNNGG 4 cut(s) 231, 270, 666, 751
BsaWI WCCGGW 1 cut(s) 224
Bsc4I CCNNNNNNNGG 2 cut(s) 354, 916
Bse118I RCCGGY 1 cut(s) 224
Bse1I ACTGG 6 cut(s) 25, 69, 74, 86, 312, 453
BseDI CCNNGG 4 cut(s) 231, 270, 666, 751
BseGI GGATG 2 cut(s) 409, 771
BseLI CCNNNNNNNGG 2 cut(s) 354, 916
BseNI ACTGG 6 cut(s) 25, 69, 74, 86, 312, 453
BseRI GAGGAG 1 cut(s) 683
BseSI GKGCMC 1 cut(s) 451
BseXI GCAGC 3 cut(s) 147, 917, 925
Bsh1236I CGCG 2 cut(s) 61, 233
BshNI GGYRCC 1 cut(s) 368
BshTI ACCGGT 1 cut(s) 224
BsiHKAI GWGCWC 2 cut(s) 451, 495
BsiSI CCGG 3 cut(s) 225, 348, 736
BsiWI CGTACG 1 cut(s) 524
BslI CCNNNNNNNGG 2 cut(s) 354, 916
BsmAI GTCTC 1 cut(s) 258
BsmI GAATGC 1 cut(s) 737
Bsp1286I GDGCHC 2 cut(s) 451, 495
Bsp143I GATC 5 cut(s) 113, 321, 553, 682, 878
Bsp19I CCATGG 1 cut(s) 751
BspACI CCGC 3 cut(s) 231, 233, 401
BspFNI CGCG 2 cut(s) 61, 233
BspHI TCATGA 1 cut(s) 514
BspLI GGNNCC 3 cut(s) 15, 229, 370
BspMAI CTGCAG 1 cut(s) 915
BspPI GGATC 3 cut(s) 329, 690, 886
BspT107I GGYRCC 1 cut(s) 368
BsrFI RCCGGY 1 cut(s) 224
BsrI ACTGG 6 cut(s) 25, 69, 74, 86, 312, 453
BssAI RCCGGY 1 cut(s) 224
BssECI CCNNGG 4 cut(s) 231, 270, 666, 751
BssMI GATC 5 cut(s) 113, 321, 553, 682, 878
BssT1I CCWWGG 2 cut(s) 270, 751
Bst4CI ACNGT 5 cut(s) 37, 194, 473, 529, 748
BstAPI GCANNNNNTGC 2 cut(s) 446, 455
BstC8I GCNNGC 1 cut(s) 140
BstDEI CTNAG 1 cut(s) 689
BstDSI CCRYGG 2 cut(s) 231, 751
BstENI CCTNNNNNAGG 1 cut(s) 914
BstF5I GGATG 2 cut(s) 409, 771
BstFNI CGCG 2 cut(s) 61, 233
BstKTI GATC 5 cut(s) 116, 324, 556, 685, 881
BstMAI GTCTC 1 cut(s) 258
BstMBI GATC 5 cut(s) 113, 321, 553, 682, 878
BstMWI GCNNNNNNNGC 2 cut(s) 446, 455
BstSCI CCNGG 1 cut(s) 735
BstSFI CTRYAG 1 cut(s) 911
BstSLI GKGCMC 1 cut(s) 451
BstUI CGCG 2 cut(s) 61, 233
BstV1I GCAGC 3 cut(s) 147, 917, 925
BtgI CCRYGG 2 cut(s) 231, 751
BtgZI GCGATG 2 cut(s) 104, 498
BtsCI GGATG 2 cut(s) 409, 771
BtsI GCAGTG 1 cut(s) 130
BtsIMutI CAGTG 3 cut(s) 130, 305, 460
Cac8I GCNNGC 1 cut(s) 140
CaiI CAGNNNCTG 1 cut(s) 106
CciI TCATGA 1 cut(s) 514
Cfr10I RCCGGY 1 cut(s) 224
Cfr13I GGNCC 4 cut(s) 14, 43, 72, 235
Cfr42I CCGCGG 1 cut(s) 234
Csp6I GTAC 5 cut(s) 179, 427, 525, 693, 853
CspAI ACCGGT 1 cut(s) 224
CviAII CATG 4 cut(s) 254, 515, 711, 752
CviJI RGCY 6 cut(s) 106, 202, 842, 869, 908, 926
CviKI_1 RGCY 6 cut(s) 106, 202, 842, 869, 908, 926
CviQI GTAC 5 cut(s) 179, 427, 525, 693, 853
DdeI CTNAG 1 cut(s) 689
DpnI GATC 5 cut(s) 115, 323, 555, 684, 880
DpnII GATC 5 cut(s) 113, 321, 553, 682, 878
DraIII CACNNNGTG 2 cut(s) 418, 455
EciI GGCGGA 1 cut(s) 416
Eco130I CCWWGG 2 cut(s) 270, 751
Eco47I GGWCC 4 cut(s) 14, 43, 72, 235
Eco57I CTGAAG 2 cut(s) 578, 701
EcoNI CCTNNNNNAGG 1 cut(s) 914
EcoT14I CCWWGG 2 cut(s) 270, 751
EcoT22I ATGCAT 1 cut(s) 790
ErhI CCWWGG 2 cut(s) 270, 751
FaeI CATG 4 cut(s) 257, 518, 714, 755
FatI CATG 4 cut(s) 253, 514, 710, 751
Fnu4HI GCNGC 3 cut(s) 136, 906, 914
FokI GGATG 2 cut(s) 416, 758
Fsp4HI GCNGC 3 cut(s) 136, 906, 914
FspBI CTAG 1 cut(s) 937
GluI GCNGC 3 cut(s) 136, 906, 914
HapII CCGG 3 cut(s) 225, 348, 736
Hin1II CATG 4 cut(s) 257, 518, 714, 755
HindIII AAGCTT 1 cut(s) 200
HinfI GANTC 1 cut(s) 7
HpaII CCGG 3 cut(s) 225, 348, 736
HphI GGTGA 3 cut(s) 206, 301, 754
Hpy166II GTNNAC 5 cut(s) 43, 292, 449, 855, 892
Hpy188I TCNGA 4 cut(s) 76, 393, 558, 720
Hpy188III TCNNGA 5 cut(s) 263, 515, 543, 607, 642
Hpy8I GTNNAC 5 cut(s) 43, 292, 449, 855, 892
Hpy99I CGWCG 2 cut(s) 767, 890
HpyAV CCTTC 7 cut(s) 311, 340, 359, 604, 608, 725, 830
HpyCH4III ACNGT 5 cut(s) 37, 194, 473, 529, 748
HpyCH4IV ACGT 2 cut(s) 445, 547
HpyCH4V TGCA 8 cut(s) 128, 305, 412, 449, 458, 703, 788, 913
HpyF10VI GCNNNNNNNGC 2 cut(s) 446, 455
HpyF3I CTNAG 1 cut(s) 689
HpySE526I ACGT 2 cut(s) 445, 547
Hsp92II CATG 4 cut(s) 257, 518, 714, 755
KspI CCGCGG 1 cut(s) 234
Kzo9I GATC 5 cut(s) 113, 321, 553, 682, 878
LmnI GCTCC 2 cut(s) 103, 498
Lsp1109I GCAGC 3 cut(s) 147, 917, 925
LweI GCATC 5 cut(s) 127, 475, 576, 775, 871
MaeI CTAG 1 cut(s) 937
MaeII ACGT 2 cut(s) 445, 547
MaeIII GTNAC 4 cut(s) 213, 383, 661, 742
MalI GATC 5 cut(s) 115, 323, 555, 684, 880
MboI GATC 5 cut(s) 113, 321, 553, 682, 878
MboII GAAGA 4 cut(s) 16, 571, 584, 794
MfeI CAATTG 1 cut(s) 459
MhlI GDGCHC 2 cut(s) 451, 495
MluCI AATT 2 cut(s) 459, 821
MmeI TCCRAC 2 cut(s) 41, 445
MnlI CCTC 6 cut(s) 92, 391, 473, 661, 760, 868
Mph1103I ATGCAT 1 cut(s) 790
MseI TTAA 2 cut(s) 630, 900
MslI CAYNNNNRTG 1 cut(s) 123
MspA1I CMGCKG 1 cut(s) 233
MspI CCGG 3 cut(s) 225, 348, 736
MspR9I CCNGG 1 cut(s) 737
MunI CAATTG 1 cut(s) 459
Mva1269I GAATGC 1 cut(s) 737
MvnI CGCG 2 cut(s) 61, 233
MwoI GCNNNNNNNGC 2 cut(s) 446, 455
NciI CCSGG 1 cut(s) 737
NcoI CCATGG 1 cut(s) 751
NdeII GATC 5 cut(s) 113, 321, 553, 682, 878
NlaIII CATG 4 cut(s) 257, 518, 714, 755
NlaIV GGNNCC 3 cut(s) 15, 229, 370
NmeAIII GCCGAG 1 cut(s) 895
NmuCI GTSAC 2 cut(s) 661, 742
NsiI ATGCAT 1 cut(s) 790
PagI TCATGA 1 cut(s) 514
PctI GAATGC 1 cut(s) 737
PfeI GAWTC 1 cut(s) 7
Pfl23II CGTACG 1 cut(s) 524
PflFI GACNNNGTC 1 cut(s) 767
PinAI ACCGGT 1 cut(s) 224
PkrI GCNGC 3 cut(s) 137, 907, 915
PspLI CGTACG 1 cut(s) 524
PspN4I GGNNCC 3 cut(s) 15, 229, 370
PspPI GGNCC 4 cut(s) 14, 43, 72, 235
PstI CTGCAG 1 cut(s) 915
PstNI CAGNNNCTG 1 cut(s) 106
PsyI GACNNNGTC 1 cut(s) 767
RsaI GTAC 5 cut(s) 180, 428, 526, 694, 854
RsaNI GTAC 5 cut(s) 179, 427, 525, 693, 853
RseI CAYNNNNRTG 1 cut(s) 123
SacII CCGCGG 1 cut(s) 234
SaqAI TTAA 2 cut(s) 630, 900
SatI GCNGC 3 cut(s) 136, 906, 914
Sau3AI GATC 5 cut(s) 113, 321, 553, 682, 878
Sau96I GGNCC 4 cut(s) 14, 43, 72, 235
ScaI AGTACT 2 cut(s) 428, 694
ScrFI CCNGG 1 cut(s) 737
SduI GDGCHC 2 cut(s) 451, 495
SfaNI GCATC 5 cut(s) 127, 475, 576, 775, 871
SfcI CTRYAG 1 cut(s) 911
Sfr303I CCGCGG 1 cut(s) 234
SgrBI CCGCGG 1 cut(s) 234
SinI GGWCC 4 cut(s) 14, 43, 72, 235
SmiMI CAYNNNNRTG 1 cut(s) 123
Sse9I AATT 2 cut(s) 459, 821
SsiI CCGC 3 cut(s) 231, 233, 401
SspMI CTAG 1 cut(s) 937
StyD4I CCNGG 1 cut(s) 735
StyI CCWWGG 2 cut(s) 270, 751
TaaI ACNGT 5 cut(s) 37, 194, 473, 529, 748
TaiI ACGT 2 cut(s) 448, 550
TaqI TCGA 2 cut(s) 262, 570
TaqII GACCGA 1 cut(s) 680
TasI AATT 2 cut(s) 459, 821
TatI WGTACW 2 cut(s) 426, 692
TfiI GAWTC 1 cut(s) 7
Tru1I TTAA 2 cut(s) 630, 900
Tru9I TTAA 2 cut(s) 630, 900
TscAI CASTG 3 cut(s) 130, 312, 460
TseFI GTSAC 2 cut(s) 661, 742
TseI GCWGC 3 cut(s) 135, 905, 913
Tsp45I GTSAC 2 cut(s) 661, 742
TspDTI ATGAA 5 cut(s) 17, 330, 610, 744, 886
TspGWI ACGGA 1 cut(s) 323
TspRI CASTG 3 cut(s) 130, 312, 460
Tth111I GACNNNGTC 1 cut(s) 767
VneI GTGCAC 1 cut(s) 447
VpaK11BI GGWCC 4 cut(s) 14, 43, 72, 235
XagI CCTNNNNNAGG 1 cut(s) 914
XspI CTAG 1 cut(s) 937
ZrmI AGTACT 2 cut(s) 428, 694
Zsp2I ATGCAT 1 cut(s) 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.