Rorug02G0037200

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000515
Physical Location & Seq
Reverse (-)
2917396 .. 2917884
489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0037200.1

Sequence Viewer

Length: 489 bp
ATGTTGACGGACGTTACCGTGCTCGTGGATTTGATGAAGTTGTTTTTCTTGAAGATAAGTTTTATGGTGTACGTTCAATCTGATAGCAGAGTTTTATCTTTAAAGGTTGACACTCATGTTCACTCCAACCTAAAATTTGCTGCACGCCGCATTGAACCAGACTATTGGTACAAGAGTTATCTAGTGTATTCAGTTGAGAAAGAATTGTTGTTAGTTCATAGGCATTTTGAGGATGCGAGACGTACAGTGAAATTTGTAGTTTATAAACTGAATTTCAATAACCGTAAGTTGACTGAGATAAACACTTTAGGTGATGTTGCTCTCTTTGTGGGTGATAACTCTACATTTTCAGTGTTGGCGTCAAGCTTTCGAGGATGTCTACCAAATTGCATATACTTTACCCATGACAGTAATACGATAAAAGTACACTTGGGGCCTGATGGTCCTCGTGATTTTGGCGTGTATGATGTCGAATATAGAAGTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

162

Amino Acids

18.88

Weight (kDa)

8.49

Isoelectric Point (pI)

22.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 26 - 156 3.7e-26 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47590 AT3G47590
fragaria_vesca FvH4_1g07650 FvH4_1g07670 FvH4_1g07671 FvH4_1g07680 FvH4_1g07690 FvH4_1g07700 FvH4_1g07700
malus_domestica MD02G1080900.v1.1 MD02G1081000.v1.1 MD02G1081300.v1.1 MD02G1081400.v1.1 MD02G1094000.v1.1 MD15G1208700.v1.1
prunus_persica Prupe.7G208200_v2.0.a1 Prupe.7G208200_v2.0.a1
pyrus_communis pycom02g07440 pycom15g18480
rosa_chinensis RchiOBHm_Chr2g0093511 RchiOBHm_Chr2g0093521 RchiOBHm_Chr2g0093531 RchiOBHm_Chr2g0093541 RchiOBHm_Chr2g0093551 RchiOBHm_Chr2g0093561
rosa_laevigata RLG00000016413 RLG00000016414 RLG00000016415 RLG00000016416 RLG00000016417
rosa_multiflora Rmu_sc0004423.1_g000003 Rmu_sc0004423.1_g000004 Rmu_sc0004423.1_g000005 Rmu_sc0004423.1_g000006 Rmu_sc0006964.1_g000003 Rmu_sc0039672.1_g000001 Rmu_sc0039672.1_g000003
rosa_roxburghii Rroxscaffold_2G00147990 Rroxscaffold_2G00148000 Rroxscaffold_2G00148010 Rroxscaffold_2G00148020 Rroxscaffold_4G00313540
rosa_rugosa Rorug01G0140600.1 Rorug01G0140700.1 Rorug02G0037000 Rorug02G0037100 Rorug02G0037200 Rorug02G0037300 Rorug02G0037400 Rorug02G0037500 Rorug02G0037600
rosa_samantha Rh2AG083400 Rh2AG083500 Rh2AG083700 Rh2AG083800 Rh2AG083900 Rh2AG084000 Rh2BG084200 Rh2BG084300 Rh2BG084400 Rh2BG084500 Rh2BG084600 Rh2CG086300 Rh2CG086400 Rh2CG086500 Rh2CG086700 Rh2CG086800 Rh2CG086900 Rh2DG082200 Rh2DG082300 Rh2DG082400 Rh2DG082500 Rh2DG082600 Rh2DG082700
rosa_wichuraiana Rw1G013200 Rw2G006360 Rw2G006370 Rw2G006380 Rw2G006390 Rw2G006400 Rw2G007130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 264
AccI GTMKAC 1 cut(s) 379
AciI CCGC 1 cut(s) 148
AcsI RAATTY 3 cut(s) 134, 251, 271
AcyI GRCGYC 1 cut(s) 359
AfaI GTAC 4 cut(s) 71, 170, 244, 426
AgsI TTSAA 4 cut(s) 52, 77, 155, 277
AluBI AGCT 1 cut(s) 366
AluI AGCT 1 cut(s) 366
Alw21I GWGCWC 1 cut(s) 24
Alw26I GTCTC 1 cut(s) 232
AoxI GGCC 1 cut(s) 434
ApeKI GCWGC 1 cut(s) 140
ApoI RAATTY 3 cut(s) 134, 251, 271
AspS9I GGNCC 2 cut(s) 434, 443
AsuHPI GGTGA 2 cut(s) 323, 344
AvaII GGWCC 1 cut(s) 443
BaeI ACNNNNGTAYC 2 cut(s) 160, 193
BauI CACGAG 2 cut(s) 23, 447
Bbv12I GWGCWC 1 cut(s) 24
BbvI GCAGC 1 cut(s) 127
BccI CCATC 1 cut(s) 434
BcoDI GTCTC 1 cut(s) 232
BfaI CTAG 1 cut(s) 182
BisI GCNGC 2 cut(s) 141, 148
BlsI GCNGC 2 cut(s) 142, 149
Bme18I GGWCC 1 cut(s) 443
BmgT120I GGNCC 2 cut(s) 434, 443
BmiI GGNNCC 1 cut(s) 435
BmsI GCATC 1 cut(s) 223
BsaHI GRCGYC 1 cut(s) 359
BseGI GGATG 2 cut(s) 238, 380
BseMII CTCAG 1 cut(s) 285
BseXI GCAGC 1 cut(s) 127
BsgI GTGCAG 1 cut(s) 126
BshFI GGCC 1 cut(s) 436
BsiHKAI GWGCWC 1 cut(s) 24
BsmAI GTCTC 1 cut(s) 232
BsmBI CGTCTC 1 cut(s) 232
BsnI GGCC 1 cut(s) 436
Bsp1286I GDGCHC 1 cut(s) 24
BspACI CCGC 1 cut(s) 148
BspANI GGCC 1 cut(s) 436
BspCNI CTCAG 1 cut(s) 286
BspLI GGNNCC 1 cut(s) 435
BssNI GRCGYC 1 cut(s) 359
BssSI CACGAG 2 cut(s) 23, 447
Bst2BI CACGAG 2 cut(s) 23, 447
Bst4CI ACNGT 4 cut(s) 19, 247, 284, 410
BstACI GRCGYC 1 cut(s) 359
BstC8I GCNNGC 1 cut(s) 145
BstDEI CTNAG 1 cut(s) 294
BstF5I GGATG 2 cut(s) 238, 380
BstMAI GTCTC 1 cut(s) 232
BstV1I GCAGC 1 cut(s) 127
BstXI CCANNNNNNTGG 1 cut(s) 165
BsuRI GGCC 1 cut(s) 436
BtsCI GGATG 2 cut(s) 238, 380
BtsIMutI CAGTG 2 cut(s) 252, 357
Cac8I GCNNGC 1 cut(s) 145
Cfr13I GGNCC 2 cut(s) 434, 443
CseI GACGC 1 cut(s) 348
Csp6I GTAC 4 cut(s) 70, 169, 243, 425
CviAII CATG 2 cut(s) 116, 404
CviJI RGCY 2 cut(s) 366, 436
CviKI_1 RGCY 2 cut(s) 366, 436
CviQI GTAC 4 cut(s) 70, 169, 243, 425
DdeI CTNAG 1 cut(s) 294
DraI TTTAAA 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 443
EcoO109I RGGNCCY 1 cut(s) 434
Esp3I CGTCTC 1 cut(s) 232
FaeI CATG 2 cut(s) 119, 407
FaiI YATR 9 cut(s) 65, 117, 219, 264, 392, 394, 405, 465, 477
FatI CATG 2 cut(s) 115, 403
FblI GTMKAC 1 cut(s) 379
Fnu4HI GCNGC 2 cut(s) 141, 148
FokI GGATG 2 cut(s) 245, 387
Fsp4HI GCNGC 2 cut(s) 141, 148
FspBI CTAG 1 cut(s) 182
GluI GCNGC 2 cut(s) 141, 148
HaeIII GGCC 1 cut(s) 436
HgaI GACGC 1 cut(s) 348
Hin1I GRCGYC 1 cut(s) 359
Hin1II CATG 2 cut(s) 119, 407
HincII GTYRAC 3 cut(s) 6, 109, 291
HindII GTYRAC 3 cut(s) 6, 109, 291
HindIII AAGCTT 1 cut(s) 364
HphI GGTGA 2 cut(s) 323, 344
Hpy166II GTNNAC 7 cut(s) 6, 70, 109, 121, 291, 380, 427
Hpy188I TCNGA 1 cut(s) 82
Hpy188III TCNNGA 2 cut(s) 49, 449
Hpy8I GTNNAC 7 cut(s) 6, 70, 109, 121, 291, 380, 427
HpyCH4III ACNGT 4 cut(s) 19, 247, 284, 410
HpyCH4IV ACGT 3 cut(s) 12, 72, 241
HpyCH4V TGCA 2 cut(s) 143, 390
HpyF3I CTNAG 1 cut(s) 294
HpySE526I ACGT 3 cut(s) 12, 72, 241
Hsp92I GRCGYC 1 cut(s) 359
Hsp92II CATG 2 cut(s) 119, 407
LpnPI CCDG 2 cut(s) 171, 450
Lsp1109I GCAGC 1 cut(s) 127
LweI GCATC 1 cut(s) 223
MaeI CTAG 1 cut(s) 182
MaeII ACGT 3 cut(s) 12, 72, 241
MaeIII GTNAC 1 cut(s) 13
MboII GAAGA 1 cut(s) 64
MhlI GDGCHC 1 cut(s) 24
MluCI AATT 5 cut(s) 134, 203, 251, 271, 385
MmeI TCCRAC 1 cut(s) 150
MnlI CCTC 3 cut(s) 223, 365, 456
MseI TTAA 2 cut(s) 101, 487
NlaIII CATG 2 cut(s) 119, 407
NlaIV GGNNCC 1 cut(s) 435
PkrI GCNGC 2 cut(s) 142, 149
PsiI TTATAA 1 cut(s) 264
PspN4I GGNNCC 1 cut(s) 435
PspPI GGNCC 2 cut(s) 434, 443
RsaI GTAC 4 cut(s) 71, 170, 244, 426
RsaNI GTAC 4 cut(s) 70, 169, 243, 425
SaqAI TTAA 2 cut(s) 101, 487
SatI GCNGC 2 cut(s) 141, 148
Sau96I GGNCC 2 cut(s) 434, 443
SduI GDGCHC 1 cut(s) 24
SetI ASST 7 cut(s) 15, 75, 108, 132, 244, 313, 368
SfaNI GCATC 1 cut(s) 223
SinI GGWCC 1 cut(s) 443
Sse9I AATT 5 cut(s) 134, 203, 251, 271, 385
SsiI CCGC 1 cut(s) 148
SspMI CTAG 1 cut(s) 182
TaaI ACNGT 4 cut(s) 19, 247, 284, 410
TaiI ACGT 3 cut(s) 15, 75, 244
TaqI TCGA 2 cut(s) 370, 471
TasI AATT 5 cut(s) 134, 203, 251, 271, 385
TatI WGTACW 1 cut(s) 424
TauI GCSGC 1 cut(s) 150
Tru1I TTAA 2 cut(s) 101, 487
Tru9I TTAA 2 cut(s) 101, 487
TscAI CASTG 2 cut(s) 252, 357
TseI GCWGC 1 cut(s) 140
TspDTI ATGAA 2 cut(s) 50, 206
TspGWI ACGGA 1 cut(s) 23
TspRI CASTG 2 cut(s) 252, 357
VpaK11BI GGWCC 1 cut(s) 443
XapI RAATTY 3 cut(s) 134, 251, 271
XmiI GTMKAC 1 cut(s) 379
XspI CTAG 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.