ACT domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 131 |
| AcsI | RAATTY | 1 cut(s) | 115 |
| AluBI | AGCT | 1 cut(s) | 14 |
| AluI | AGCT | 1 cut(s) | 14 |
| ApoI | RAATTY | 1 cut(s) | 115 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 139 |
| BccI | CCATC | 2 cut(s) | 149, 167 |
| BfmI | CTRYAG | 1 cut(s) | 150 |
| BseGI | GGATG | 1 cut(s) | 178 |
| BseYI | CCCAGC | 1 cut(s) | 6 |
| BspMAI | CTGCAG | 1 cut(s) | 154 |
| BstC8I | GCNNGC | 1 cut(s) | 12 |
| BstF5I | GGATG | 1 cut(s) | 178 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 20 |
| BstSFI | CTRYAG | 1 cut(s) | 150 |
| BtsCI | GGATG | 1 cut(s) | 178 |
| Cac8I | GCNNGC | 1 cut(s) | 12 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 63, 98 |
| CviAII | CATG | 1 cut(s) | 169 |
| CviJI | RGCY | 5 cut(s) | 10, 14, 36, 50, 140 |
| CviKI_1 | RGCY | 5 cut(s) | 10, 14, 36, 50, 140 |
| FaeI | CATG | 1 cut(s) | 172 |
| FaiI | YATR | 3 cut(s) | 17, 131, 170 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 124, 156 |
| FatI | CATG | 1 cut(s) | 168 |
| FokI | GGATG | 1 cut(s) | 185 |
| GsaI | CCCAGC | 1 cut(s) | 10 |
| Hin1II | CATG | 1 cut(s) | 172 |
| Hpy188I | TCNGA | 1 cut(s) | 113 |
| HpyAV | CCTTC | 1 cut(s) | 129 |
| HpyCH4V | TGCA | 2 cut(s) | 74, 152 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 20 |
| Hsp92II | CATG | 1 cut(s) | 172 |
| LpnPI | CCDG | 4 cut(s) | 20, 24, 28, 91 |
| MfeI | CAATTG | 1 cut(s) | 193 |
| MluCI | AATT | 3 cut(s) | 115, 193, 202 |
| MroXI | GAANNNNTTC | 1 cut(s) | 139 |
| MseI | TTAA | 1 cut(s) | 60 |
| MunI | CAATTG | 1 cut(s) | 193 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 20 |
| NlaIII | CATG | 1 cut(s) | 172 |
| PdmI | GAANNNNTTC | 1 cut(s) | 139 |
| PsiI | TTATAA | 1 cut(s) | 131 |
| PspFI | CCCAGC | 1 cut(s) | 6 |
| PstI | CTGCAG | 1 cut(s) | 154 |
| SaqAI | TTAA | 1 cut(s) | 60 |
| SetI | ASST | 4 cut(s) | 16, 66, 110, 183 |
| SfcI | CTRYAG | 1 cut(s) | 150 |
| SgeI | CNNG | 7 cut(s) | 19, 23, 51, 55, 109, 118, 181 |
| Sse9I | AATT | 3 cut(s) | 115, 193, 202 |
| TasI | AATT | 3 cut(s) | 115, 193, 202 |
| Tru1I | TTAA | 1 cut(s) | 60 |
| Tru9I | TTAA | 1 cut(s) | 60 |
| TspDTI | ATGAA | 1 cut(s) | 157 |
| XapI | RAATTY | 1 cut(s) | 115 |
| XmnI | GAANNNNTTC | 1 cut(s) | 139 |