Rh2AG084000

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000515
Physical Location & Seq
Reverse (-)
7095059 .. 7097518
2460 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG084000.1

Sequence Viewer

Length: 897 bp
ATGCTGCTCTCAAATTCTTATTCTTTAACTTCCCTAAAGTTCCCATCGCCAAAACTCAGTCTTCACCAAAATCGCAGCCCAAAACGAATCGTGAAAATGGCCCAGACTGCCCAAAACCCGGTGAAGCAGCAATTGAAAATCGTTGTACCCAACAAACATGGTGAAAAACTTGTGGGCTTATTACATGACACCGGTTCTGGGGAGATTGTAATCTTATGTCATGGTTTTCGATCCTCAAAGGAGACAACTAGTATTGCAAACATTGCTGTTGCGTTGGAGAATGAAGGAATTAGCTCCTTCCGTTTTGACTTTGCTGGAAATGGGGAAAGTGAAGGTACCTTTCAGTATGGTCATTATCGTAGAGAGGCTGATGACTTGCATGCTGTAGTCCAACACTTCTCTGGGGCAAATCGTGTACCAAGTGCAATCCTTGGGCACAGTAAAGGAGGTAATGTTGTGCTCCTATATGCTTCCAAGTATCATGACATTCCTACCGTTGTCAATGTTTCTGGGCGTTATGATATGAAAAGAGGCATTAAAGAACGCTTAGGAGAAGACTTTATGCAAAGAATCAAGAAGGAAGGATTTATTGATGTTCAGAATAAGAGAGGAAGTTATCGAGTGACAGAGGAAAGTTTGATGGATCGCCTAAGTACAGATATGCATGAAGCATGCCTTCAGATTGACAAAGATTGTCGCGTATTAACTGTCCATGGGTCTGCTGACGAAATCATCGCTGTTGAAGACGCATTAGAGTTTGCCAAGATTATACCTAACCACAAACTACATATCATAGAGGGAGCTAACCATAACTACAGCTCACATCAAGCCGAGTTGGCGTCAGTTGTTGTGGACTTCATAAAGACATCTCTGCAGCAGGACAAGGCTACTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

298

Amino Acids

33.11

Weight (kDa)

7.81

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 69 - 181 3.4e-14 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 69 - 171 5.7e-07 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 70 - 280 6.6e-06 Alpha/beta hydrolase family
Peptidase_S9 PF00326 120 - 292 9.3e-06 Prolyl oligopeptidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47590 AT3G47590
fragaria_vesca FvH4_1g07650 FvH4_1g07670 FvH4_1g07671 FvH4_1g07680 FvH4_1g07690 FvH4_1g07700 FvH4_1g07700
malus_domestica MD02G1080900.v1.1 MD02G1081000.v1.1 MD02G1081300.v1.1 MD02G1081400.v1.1 MD02G1094000.v1.1 MD15G1208700.v1.1
prunus_persica Prupe.7G208200_v2.0.a1 Prupe.7G208200_v2.0.a1
pyrus_communis pycom02g07440 pycom15g18480
rosa_chinensis RchiOBHm_Chr2g0093511 RchiOBHm_Chr2g0093521 RchiOBHm_Chr2g0093531 RchiOBHm_Chr2g0093541 RchiOBHm_Chr2g0093551 RchiOBHm_Chr2g0093561
rosa_laevigata RLG00000016413 RLG00000016414 RLG00000016415 RLG00000016416 RLG00000016417
rosa_multiflora Rmu_sc0004423.1_g000003 Rmu_sc0004423.1_g000004 Rmu_sc0004423.1_g000005 Rmu_sc0004423.1_g000006 Rmu_sc0006964.1_g000003 Rmu_sc0039672.1_g000001 Rmu_sc0039672.1_g000003
rosa_roxburghii Rroxscaffold_2G00147990 Rroxscaffold_2G00148000 Rroxscaffold_2G00148010 Rroxscaffold_2G00148020 Rroxscaffold_4G00313540
rosa_rugosa Rorug01G0140600.1 Rorug01G0140700.1 Rorug02G0037000 Rorug02G0037100 Rorug02G0037200 Rorug02G0037300 Rorug02G0037400 Rorug02G0037500 Rorug02G0037600
rosa_samantha Rh2AG083400 Rh2AG083500 Rh2AG083700 Rh2AG083800 Rh2AG083900 Rh2AG084000 Rh2BG084200 Rh2BG084300 Rh2BG084400 Rh2BG084500 Rh2BG084600 Rh2CG086300 Rh2CG086400 Rh2CG086500 Rh2CG086700 Rh2CG086800 Rh2CG086900 Rh2DG082200 Rh2DG082300 Rh2DG082400 Rh2DG082500 Rh2DG082600 Rh2DG082700
rosa_wichuraiana Rw1G013200 Rw2G006360 Rw2G006370 Rw2G006380 Rw2G006390 Rw2G006400 Rw2G007130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 335
AccB1I GGYRCC 1 cut(s) 335
AccII CGCG 1 cut(s) 699
AclWI GGATC 2 cut(s) 225, 651
AcsI RAATTY 1 cut(s) 13
AcuI CTGAAG 1 cut(s) 662
AcyI GRCGYC 1 cut(s) 839
AfaI GTAC 4 cut(s) 147, 337, 417, 655
AfiI CCNNNNNNNGG 2 cut(s) 118, 198
AgeI ACCGGT 1 cut(s) 191
AgsI TTSAA 2 cut(s) 136, 743
AhlI ACTAGT 1 cut(s) 248
AluBI AGCT 3 cut(s) 294, 803, 819
AluI AGCT 3 cut(s) 294, 803, 819
Alw21I GWGCWC 1 cut(s) 462
Alw26I GTCTC 1 cut(s) 236
AlwI GGATC 2 cut(s) 225, 651
AoxI GGCC 1 cut(s) 99
ApeKI GCWGC 4 cut(s) 4, 75, 127, 874
ApoI RAATTY 1 cut(s) 13
AsiGI ACCGGT 1 cut(s) 191
Asp718I GGTACC 1 cut(s) 335
AspS9I GGNCC 1 cut(s) 100
AsuC2I CCSGG 1 cut(s) 119
AsuHPI GGTGA 3 cut(s) 56, 133, 173
BaeGI GKGCMC 1 cut(s) 438
BanI GGYRCC 1 cut(s) 335
BbsI GAAGAC 3 cut(s) 53, 561, 750
Bbv12I GWGCWC 1 cut(s) 462
BbvI GCAGC 3 cut(s) 87, 139, 886
BccI CCATC 2 cut(s) 52, 634
BcnI CCSGG 1 cut(s) 119
BcoDI GTCTC 1 cut(s) 236
BcuI ACTAGT 1 cut(s) 248
BfaI CTAG 1 cut(s) 249
BfmI CTRYAG 3 cut(s) 384, 814, 872
BglI GCCNNNNNGGC 1 cut(s) 836
BisI GCNGC 4 cut(s) 5, 76, 128, 875
BlsI GCNGC 4 cut(s) 6, 77, 129, 876
Bme1390I CCNGG 1 cut(s) 119
BmgT120I GGNCC 1 cut(s) 100
BmiI GGNNCC 1 cut(s) 337
BmrFI CCNGG 1 cut(s) 119
BpiI GAAGAC 3 cut(s) 53, 561, 750
Bpu10I CCTNAGC 1 cut(s) 547
BpuMI CCSGG 1 cut(s) 119
BsaBI GATNNNNATC 1 cut(s) 209
BsaHI GRCGYC 1 cut(s) 839
BsaJI CCNNGG 2 cut(s) 430, 712
BsaWI WCCGGW 1 cut(s) 191
BsaXI ACNNNNNCTCC 2 cut(s) 438, 468
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 198
Bse118I RCCGGY 1 cut(s) 191
Bse3DI GCAATG 1 cut(s) 261
Bse8I GATNNNNATC 1 cut(s) 209
BseDI CCNNGG 2 cut(s) 430, 712
BseJI GATNNNNATC 1 cut(s) 209
BseLI CCNNNNNNNGG 2 cut(s) 118, 198
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 1 cut(s) 70
BseSI GKGCMC 1 cut(s) 438
BseXI GCAGC 3 cut(s) 87, 139, 886
Bsh1236I CGCG 1 cut(s) 699
BshFI GGCC 1 cut(s) 101
BshNI GGYRCC 1 cut(s) 335
BshTI ACCGGT 1 cut(s) 191
BsiHKAI GWGCWC 1 cut(s) 462
BsiSI CCGG 2 cut(s) 119, 192
BslI CCNNNNNNNGG 2 cut(s) 118, 198
BsmAI GTCTC 1 cut(s) 236
BsnI GGCC 1 cut(s) 101
Bsp1286I GDGCHC 2 cut(s) 438, 462
Bsp143I GATC 2 cut(s) 230, 643
Bsp19I CCATGG 1 cut(s) 712
BspANI GGCC 1 cut(s) 101
BspCNI CTCAG 1 cut(s) 69
BspFNI CGCG 1 cut(s) 699
BspHI TCATGA 1 cut(s) 481
BspLI GGNNCC 1 cut(s) 337
BspMAI CTGCAG 1 cut(s) 876
BspPI GGATC 2 cut(s) 225, 651
BspT107I GGYRCC 1 cut(s) 335
BsrDI GCAATG 1 cut(s) 261
BsrFI RCCGGY 1 cut(s) 191
BssAI RCCGGY 1 cut(s) 191
BssECI CCNNGG 2 cut(s) 430, 712
BssMI GATC 2 cut(s) 230, 643
BssNI GRCGYC 1 cut(s) 839
BssT1I CCWWGG 2 cut(s) 430, 712
Bst4CI ACNGT 3 cut(s) 440, 496, 709
BstACI GRCGYC 1 cut(s) 839
BstAPI GCANNNNNTGC 1 cut(s) 263
BstC8I GCNNGC 2 cut(s) 381, 673
BstDEI CTNAG 3 cut(s) 56, 547, 650
BstDSI CCRYGG 1 cut(s) 712
BstFNI CGCG 1 cut(s) 699
BstKTI GATC 2 cut(s) 233, 646
BstMAI GTCTC 1 cut(s) 236
BstMBI GATC 2 cut(s) 230, 643
BstMWI GCNNNNNNNGC 3 cut(s) 107, 263, 836
BstNSI RCATGY 2 cut(s) 383, 675
BstSCI CCNGG 1 cut(s) 117
BstSFI CTRYAG 3 cut(s) 384, 814, 872
BstSLI GKGCMC 1 cut(s) 438
BstUI CGCG 1 cut(s) 699
BstV1I GCAGC 3 cut(s) 87, 139, 886
BstV2I GAAGAC 3 cut(s) 53, 561, 750
BsuRI GGCC 1 cut(s) 101
BtgI CCRYGG 1 cut(s) 712
BtgZI GCGATG 2 cut(s) 30, 718
Cac8I GCNNGC 2 cut(s) 381, 673
CciI TCATGA 1 cut(s) 481
Cfr10I RCCGGY 1 cut(s) 191
Cfr13I GGNCC 1 cut(s) 100
CseI GACGC 2 cut(s) 755, 828
Csp6I GTAC 4 cut(s) 146, 336, 416, 654
CspAI ACCGGT 1 cut(s) 191
CviAII CATG 8 cut(s) 158, 185, 221, 380, 482, 665, 672, 713
CviJI RGCY 9 cut(s) 78, 101, 177, 294, 368, 803, 819, 830, 887
CviKI_1 RGCY 9 cut(s) 78, 101, 177, 294, 368, 803, 819, 830, 887
CviQI GTAC 4 cut(s) 146, 336, 416, 654
DdeI CTNAG 3 cut(s) 56, 547, 650
DpnI GATC 2 cut(s) 232, 645
DpnII GATC 2 cut(s) 230, 643
Eco130I CCWWGG 2 cut(s) 430, 712
Eco57I CTGAAG 1 cut(s) 662
EcoT14I CCWWGG 2 cut(s) 430, 712
EcoT22I ATGCAT 1 cut(s) 666
ErhI CCWWGG 2 cut(s) 430, 712
FaeI CATG 8 cut(s) 161, 188, 224, 383, 485, 668, 675, 716
FalI AAGNNNNNCTT 2 cut(s) 660, 692
FatI CATG 8 cut(s) 157, 184, 220, 379, 481, 664, 671, 712
Fnu4HI GCNGC 4 cut(s) 5, 76, 128, 875
Fsp4HI GCNGC 4 cut(s) 5, 76, 128, 875
FspBI CTAG 1 cut(s) 249
GluI GCNGC 4 cut(s) 5, 76, 128, 875
HaeIII GGCC 1 cut(s) 101
HapII CCGG 2 cut(s) 119, 192
HgaI GACGC 2 cut(s) 755, 828
Hin1I GRCGYC 1 cut(s) 839
Hin1II CATG 8 cut(s) 161, 188, 224, 383, 485, 668, 675, 716
HinfI GANTC 2 cut(s) 87, 570
HpaII CCGG 2 cut(s) 119, 192
HphI GGTGA 3 cut(s) 56, 133, 173
Hpy166II GTNNAC 2 cut(s) 416, 853
Hpy188I TCNGA 2 cut(s) 600, 681
Hpy188III TCNNGA 3 cut(s) 91, 482, 574
Hpy8I GTNNAC 2 cut(s) 416, 853
HpyAV CCTTC 6 cut(s) 278, 307, 326, 571, 575, 686
HpyCH4III ACNGT 3 cut(s) 440, 496, 709
HpyCH4V TGCA 6 cut(s) 257, 379, 425, 565, 664, 874
HpyF10VI GCNNNNNNNGC 3 cut(s) 107, 263, 836
HpyF3I CTNAG 3 cut(s) 56, 547, 650
Hsp92I GRCGYC 1 cut(s) 839
Hsp92II CATG 8 cut(s) 161, 188, 224, 383, 485, 668, 675, 716
KpnI GGTACC 1 cut(s) 339
Kzo9I GATC 2 cut(s) 230, 643
LmnI GCTCC 3 cut(s) 299, 465, 800
LpnPI CCDG 8 cut(s) 116, 132, 183, 205, 300, 387, 495, 863
Lsp1109I GCAGC 3 cut(s) 87, 139, 886
MaeI CTAG 1 cut(s) 249
MaeIII GTNAC 1 cut(s) 622
MalI GATC 2 cut(s) 232, 645
MboI GATC 2 cut(s) 230, 643
MboII GAAGA 3 cut(s) 53, 566, 755
MfeI CAATTG 1 cut(s) 131
MhlI GDGCHC 2 cut(s) 438, 462
MluCI AATT 3 cut(s) 13, 131, 288
MmeI TCCRAC 2 cut(s) 255, 415
MnlI CCTC 7 cut(s) 244, 358, 440, 524, 602, 622, 790
Mph1103I ATGCAT 1 cut(s) 666
MseI TTAA 4 cut(s) 26, 537, 704, 895
MspI CCGG 2 cut(s) 119, 192
MspR9I CCNGG 1 cut(s) 119
MunI CAATTG 1 cut(s) 131
MvnI CGCG 1 cut(s) 699
MwoI GCNNNNNNNGC 3 cut(s) 107, 263, 836
NciI CCSGG 1 cut(s) 119
NcoI CCATGG 1 cut(s) 712
NdeII GATC 2 cut(s) 230, 643
NlaIII CATG 8 cut(s) 161, 188, 224, 383, 485, 668, 675, 716
NlaIV GGNNCC 1 cut(s) 337
NmeAIII GCCGAG 1 cut(s) 856
NmuCI GTSAC 1 cut(s) 622
NsiI ATGCAT 1 cut(s) 666
NspI RCATGY 2 cut(s) 383, 675
PaeI GCATGC 2 cut(s) 383, 675
PagI TCATGA 1 cut(s) 481
PfeI GAWTC 2 cut(s) 87, 570
PinAI ACCGGT 1 cut(s) 191
PkrI GCNGC 4 cut(s) 6, 77, 129, 876
PspN4I GGNNCC 1 cut(s) 337
PspPI GGNCC 1 cut(s) 100
PstI CTGCAG 1 cut(s) 876
RsaI GTAC 4 cut(s) 147, 337, 417, 655
RsaNI GTAC 4 cut(s) 146, 336, 416, 654
SaqAI TTAA 4 cut(s) 26, 537, 704, 895
SatI GCNGC 4 cut(s) 5, 76, 128, 875
Sau3AI GATC 2 cut(s) 230, 643
Sau96I GGNCC 1 cut(s) 100
ScrFI CCNGG 1 cut(s) 119
SduI GDGCHC 2 cut(s) 438, 462
SetI ASST 7 cut(s) 296, 337, 341, 451, 775, 805, 821
SfcI CTRYAG 3 cut(s) 384, 814, 872
SpeI ACTAGT 1 cut(s) 248
SphI GCATGC 2 cut(s) 383, 675
Sse9I AATT 3 cut(s) 13, 131, 288
SspMI CTAG 1 cut(s) 249
StyD4I CCNGG 1 cut(s) 117
StyI CCWWGG 2 cut(s) 430, 712
TaaI ACNGT 3 cut(s) 440, 496, 709
TaqI TCGA 2 cut(s) 229, 619
TasI AATT 3 cut(s) 13, 131, 288
TatI WGTACW 1 cut(s) 653
TfiI GAWTC 2 cut(s) 87, 570
Tru1I TTAA 4 cut(s) 26, 537, 704, 895
Tru9I TTAA 4 cut(s) 26, 537, 704, 895
TseFI GTSAC 1 cut(s) 622
TseI GCWGC 4 cut(s) 4, 75, 127, 874
Tsp45I GTSAC 1 cut(s) 622
TspDTI ATGAA 4 cut(s) 297, 539, 681, 847
TspGWI ACGGA 1 cut(s) 290
XapI RAATTY 1 cut(s) 13
XceI RCATGY 2 cut(s) 383, 675
XcmI CCANNNNNNNNNTGG 1 cut(s) 398
XspI CTAG 1 cut(s) 249
Zsp2I ATGCAT 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.