MD02G1081000.v1.1

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000515
Physical Location & Seq
Reverse (-)
6369899 .. 6371861
1963 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1081000.v1.1.491

Sequence Viewer

Length: 810 bp
ATGGCAGAAGCTGAACAAAACCCAGCAGTAGCTCCGCAACAGAAAATCGTTATACCCAACAAACATGGCGAAAAGCTCGTGGGCATATTACATGAAACCGGTTCTGGGGAGATTGTAATCTTATGTCATGGTTTTCGAGCCTCCAAGGTTGAAGAAACAACTATGGTGAACCTTGCTGTTGCATTGGAAAAGGAAGGAATTAGTTCCTTCCGTTTCGACTTTGCTGGAAATGGAGAGAGTGAAGGCACCTTTCTGTATGGCAACTATCGGAGAGAGGCCGAGGACTTGCATGCTGTGGTCCAACACTTCTCTAGGGCAAACCGGCCACCAACTGCAATTCTTGGGCACAGTAAAGGAGCTGGTGTGGTGCTCCTGTATGCTTCTAAGTATCATGACATTCGTACGGTTGTCAATCTTTCTGGACGTTATGATCTGAAGAAAAACATTGCACCACAATTGGGGAAAGACTTCATGGAAATAATCAAGAAGGAAGGATTCATTGATGTTAAAACTAAGACAGGAAGTGTGATTTACCGTGTGACCGAGGAAGGTCTGATGGATCGCCTAAGTACTGATATGCACGAATCATGCCTTCATATTGACAAAGAATGCCGGGTGTTGACAGTCCATGGTTCTGCTGATGAGGCCATCCCTGTCGAAGATGCATTCGAGTTTTCAAAGATAATACCAAACCACAAATTACATGTTATCGAAGGAGCTAACCATTGTTACACCTCACATCAAGCTGAGTTAGCGACAGTCGTTTTGGACTTCATAAAAGCAGCTCTGCAGCAGAACAAGGTTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

270

Amino Acids

29.77

Weight (kDa)

6.16

Isoelectric Point (pI)

35.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 38 - 161 1.9e-14 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 38 - 141 1.5e-08 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 39 - 252 1.2e-06 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47590 AT3G47590
fragaria_vesca FvH4_1g07650 FvH4_1g07670 FvH4_1g07671 FvH4_1g07680 FvH4_1g07690 FvH4_1g07700 FvH4_1g07700
malus_domestica MD02G1080900.v1.1 MD02G1081000.v1.1 MD02G1081300.v1.1 MD02G1081400.v1.1 MD02G1094000.v1.1 MD15G1208700.v1.1
prunus_persica Prupe.7G208200_v2.0.a1 Prupe.7G208200_v2.0.a1
pyrus_communis pycom02g07440 pycom15g18480
rosa_chinensis RchiOBHm_Chr2g0093511 RchiOBHm_Chr2g0093521 RchiOBHm_Chr2g0093531 RchiOBHm_Chr2g0093541 RchiOBHm_Chr2g0093551 RchiOBHm_Chr2g0093561
rosa_laevigata RLG00000016413 RLG00000016414 RLG00000016415 RLG00000016416 RLG00000016417
rosa_multiflora Rmu_sc0004423.1_g000003 Rmu_sc0004423.1_g000004 Rmu_sc0004423.1_g000005 Rmu_sc0004423.1_g000006 Rmu_sc0006964.1_g000003 Rmu_sc0039672.1_g000001 Rmu_sc0039672.1_g000003
rosa_roxburghii Rroxscaffold_2G00147990 Rroxscaffold_2G00148000 Rroxscaffold_2G00148010 Rroxscaffold_2G00148020 Rroxscaffold_4G00313540
rosa_rugosa Rorug01G0140600.1 Rorug01G0140700.1 Rorug02G0037000 Rorug02G0037100 Rorug02G0037200 Rorug02G0037300 Rorug02G0037400 Rorug02G0037500 Rorug02G0037600
rosa_samantha Rh2AG083400 Rh2AG083500 Rh2AG083700 Rh2AG083800 Rh2AG083900 Rh2AG084000 Rh2BG084200 Rh2BG084300 Rh2BG084400 Rh2BG084500 Rh2BG084600 Rh2CG086300 Rh2CG086400 Rh2CG086500 Rh2CG086700 Rh2CG086800 Rh2CG086900 Rh2DG082200 Rh2DG082300 Rh2DG082400 Rh2DG082500 Rh2DG082600 Rh2DG082700
rosa_wichuraiana Rw1G013200 Rw2G006360 Rw2G006370 Rw2G006380 Rw2G006390 Rw2G006400 Rw2G007130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 245
AciI CCGC 1 cut(s) 35
AclWI GGATC 1 cut(s) 567
AcoI YGGCCR 1 cut(s) 323
AcuI CTGAAG 1 cut(s) 455
AfaI GTAC 2 cut(s) 403, 571
AfiI CCNNNNNNNGG 2 cut(s) 105, 458
AflIII ACRYGT 1 cut(s) 703
AgeI ACCGGT 1 cut(s) 98
AgsI TTSAA 2 cut(s) 152, 678
AluBI AGCT 7 cut(s) 11, 32, 76, 359, 719, 746, 785
AluI AGCT 7 cut(s) 11, 32, 76, 359, 719, 746, 785
Alw21I GWGCWC 1 cut(s) 372
AlwI GGATC 1 cut(s) 567
AlwNI CAGNNNCTG 1 cut(s) 11
AoxI GGCC 3 cut(s) 276, 323, 645
ApeKI GCWGC 2 cut(s) 782, 790
ArsI GACNNNNNNTTYG 2 cut(s) 748, 780
AsiGI ACCGGT 1 cut(s) 98
Asp700I GAANNNNTTC 2 cut(s) 202, 467
AspS9I GGNCC 1 cut(s) 298
AsuC2I CCSGG 1 cut(s) 614
AsuHPI GGTGA 1 cut(s) 178
AvaII GGWCC 1 cut(s) 298
BaeGI GKGCMC 1 cut(s) 348
BanI GGYRCC 1 cut(s) 245
BauI CACGAG 1 cut(s) 77
Bbv12I GWGCWC 1 cut(s) 372
BbvI GCAGC 2 cut(s) 794, 802
BccI CCATC 2 cut(s) 550, 656
BcnI CCSGG 1 cut(s) 614
BfaI CTAG 1 cut(s) 312
BfmI CTRYAG 1 cut(s) 788
BisI GCNGC 2 cut(s) 783, 791
BlsI GCNGC 2 cut(s) 784, 792
BmcAI AGTACT 1 cut(s) 571
Bme1390I CCNGG 1 cut(s) 614
Bme18I GGWCC 1 cut(s) 298
BmgT120I GGNCC 1 cut(s) 298
BmiI GGNNCC 1 cut(s) 247
BmrFI CCNGG 1 cut(s) 614
BmsI GCATC 1 cut(s) 652
BpuMI CCSGG 1 cut(s) 614
BsaBI GATNNNNATC 1 cut(s) 116
BsaJI CCNNGG 4 cut(s) 144, 279, 543, 628
BsaWI WCCGGW 1 cut(s) 98
BsaXI ACNNNNNCTCC 2 cut(s) 348, 378
Bsc4I CCNNNNNNNGG 2 cut(s) 105, 458
Bse118I RCCGGY 2 cut(s) 98, 321
Bse3DI GCAATG 1 cut(s) 444
Bse8I GATNNNNATC 1 cut(s) 116
BseDI CCNNGG 4 cut(s) 144, 279, 543, 628
BseGI GGATG 1 cut(s) 648
BseJI GATNNNNATC 1 cut(s) 116
BseLI CCNNNNNNNGG 2 cut(s) 105, 458
BseMI GCAATG 1 cut(s) 444
BseMII CTCAG 1 cut(s) 738
BseSI GKGCMC 1 cut(s) 348
BseXI GCAGC 2 cut(s) 794, 802
BseYI CCCAGC 1 cut(s) 22
BshFI GGCC 3 cut(s) 278, 325, 647
BshNI GGYRCC 1 cut(s) 245
BshTI ACCGGT 1 cut(s) 98
BsiHKAI GWGCWC 1 cut(s) 372
BsiSI CCGG 3 cut(s) 99, 322, 613
BsiWI CGTACG 1 cut(s) 401
BslI CCNNNNNNNGG 2 cut(s) 105, 458
BsmI GAATGC 2 cut(s) 614, 665
BsnI GGCC 3 cut(s) 278, 325, 647
Bsp1286I GDGCHC 2 cut(s) 348, 372
Bsp143I GATC 2 cut(s) 430, 559
Bsp19I CCATGG 1 cut(s) 628
BspACI CCGC 1 cut(s) 35
BspANI GGCC 3 cut(s) 278, 325, 647
BspCNI CTCAG 1 cut(s) 739
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 1 cut(s) 247
BspMAI CTGCAG 1 cut(s) 792
BspPI GGATC 1 cut(s) 567
BspT107I GGYRCC 1 cut(s) 245
BsrDI GCAATG 1 cut(s) 444
BsrFI RCCGGY 2 cut(s) 98, 321
BssAI RCCGGY 2 cut(s) 98, 321
BssECI CCNNGG 4 cut(s) 144, 279, 543, 628
BssMI GATC 2 cut(s) 430, 559
BssSI CACGAG 1 cut(s) 77
BssT1I CCWWGG 2 cut(s) 144, 628
Bst2BI CACGAG 1 cut(s) 77
Bst4CI ACNGT 5 cut(s) 350, 406, 536, 625, 760
BstC8I GCNNGC 1 cut(s) 291
BstDEI CTNAG 4 cut(s) 384, 513, 566, 747
BstDSI CCRYGG 1 cut(s) 628
BstF5I GGATG 1 cut(s) 648
BstKTI GATC 2 cut(s) 433, 562
BstMBI GATC 2 cut(s) 430, 559
BstMWI GCNNNNNNNGC 2 cut(s) 644, 752
BstNSI RCATGY 2 cut(s) 293, 707
BstSCI CCNGG 1 cut(s) 612
BstSFI CTRYAG 1 cut(s) 788
BstSLI GKGCMC 1 cut(s) 348
BstV1I GCAGC 2 cut(s) 794, 802
BsuRI GGCC 3 cut(s) 278, 325, 647
BtgI CCRYGG 1 cut(s) 628
BtsCI GGATG 1 cut(s) 648
Cac8I GCNNGC 1 cut(s) 291
CaiI CAGNNNCTG 1 cut(s) 11
CciI TCATGA 1 cut(s) 391
Cfr10I RCCGGY 2 cut(s) 98, 321
Cfr13I GGNCC 1 cut(s) 298
Csp6I GTAC 2 cut(s) 402, 570
CspAI ACCGGT 1 cut(s) 98
CviAII CATG 9 cut(s) 65, 92, 128, 290, 392, 472, 588, 629, 704
CviQI GTAC 2 cut(s) 402, 570
DdeI CTNAG 4 cut(s) 384, 513, 566, 747
DpnI GATC 2 cut(s) 432, 561
DpnII GATC 2 cut(s) 430, 559
EaeI YGGCCR 1 cut(s) 323
Eco130I CCWWGG 2 cut(s) 144, 628
Eco47I GGWCC 1 cut(s) 298
Eco57I CTGAAG 1 cut(s) 455
EcoT14I CCWWGG 2 cut(s) 144, 628
EcoT22I ATGCAT 1 cut(s) 667
ErhI CCWWGG 2 cut(s) 144, 628
FaeI CATG 9 cut(s) 68, 95, 131, 293, 395, 475, 591, 632, 707
FatI CATG 9 cut(s) 64, 91, 127, 289, 391, 471, 587, 628, 703
Fnu4HI GCNGC 2 cut(s) 783, 791
FokI GGATG 1 cut(s) 635
Fsp4HI GCNGC 2 cut(s) 783, 791
FspBI CTAG 1 cut(s) 312
GluI GCNGC 2 cut(s) 783, 791
GsaI CCCAGC 1 cut(s) 26
HaeIII GGCC 3 cut(s) 278, 325, 647
HapII CCGG 3 cut(s) 99, 322, 613
Hin1II CATG 9 cut(s) 68, 95, 131, 293, 395, 475, 591, 632, 707
HincII GTYRAC 1 cut(s) 621
HindII GTYRAC 1 cut(s) 621
HinfI GANTC 2 cut(s) 495, 584
HpaII CCGG 3 cut(s) 99, 322, 613
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 2 cut(s) 169, 621
Hpy188I TCNGA 3 cut(s) 270, 435, 555
Hpy188III TCNNGA 3 cut(s) 392, 420, 484
Hpy8I GTNNAC 2 cut(s) 169, 621
HpyAV CCTTC 8 cut(s) 188, 217, 236, 481, 485, 542, 602, 707
HpyCH4III ACNGT 5 cut(s) 350, 406, 536, 625, 760
HpyCH4IV ACGT 1 cut(s) 424
HpyCH4V TGCA 7 cut(s) 182, 289, 335, 449, 580, 665, 790
HpyF10VI GCNNNNNNNGC 2 cut(s) 644, 752
HpyF3I CTNAG 4 cut(s) 384, 513, 566, 747
HpySE526I ACGT 1 cut(s) 424
Hsp92II CATG 9 cut(s) 68, 95, 131, 293, 395, 475, 591, 632, 707
Kzo9I GATC 2 cut(s) 430, 559
LmnI GCTCC 4 cut(s) 37, 356, 375, 716
Lsp1109I GCAGC 2 cut(s) 794, 802
LweI GCATC 1 cut(s) 652
MaeI CTAG 1 cut(s) 312
MaeII ACGT 1 cut(s) 424
MaeIII GTNAC 2 cut(s) 538, 728
MalI GATC 2 cut(s) 432, 561
MboI GATC 2 cut(s) 430, 559
MboII GAAGA 3 cut(s) 164, 448, 671
MfeI CAATTG 1 cut(s) 455
MhlI GDGCHC 2 cut(s) 348, 372
MluCI AATT 5 cut(s) 198, 336, 455, 698, 805
MmeI TCCRAC 1 cut(s) 325
MnlI CCTC 6 cut(s) 151, 268, 274, 538, 637, 745
Mph1103I ATGCAT 1 cut(s) 667
MroXI GAANNNNTTC 2 cut(s) 202, 467
MseI TTAA 2 cut(s) 507, 804
MspI CCGG 3 cut(s) 99, 322, 613
MspR9I CCNGG 1 cut(s) 614
MunI CAATTG 1 cut(s) 455
Mva1269I GAATGC 2 cut(s) 614, 665
MwoI GCNNNNNNNGC 2 cut(s) 644, 752
NciI CCSGG 1 cut(s) 614
NcoI CCATGG 1 cut(s) 628
NdeII GATC 2 cut(s) 430, 559
NlaIII CATG 9 cut(s) 68, 95, 131, 293, 395, 475, 591, 632, 707
NlaIV GGNNCC 1 cut(s) 247
NmeAIII GCCGAG 1 cut(s) 304
NmuCI GTSAC 1 cut(s) 538
NsiI ATGCAT 1 cut(s) 667
NspI RCATGY 2 cut(s) 293, 707
PaeI GCATGC 1 cut(s) 293
PagI TCATGA 1 cut(s) 391
PciI ACATGT 1 cut(s) 703
PctI GAATGC 2 cut(s) 614, 665
PdmI GAANNNNTTC 2 cut(s) 202, 467
PfeI GAWTC 2 cut(s) 495, 584
Pfl23II CGTACG 1 cut(s) 401
PinAI ACCGGT 1 cut(s) 98
PkrI GCNGC 2 cut(s) 784, 792
PscI ACATGT 1 cut(s) 703
PspFI CCCAGC 1 cut(s) 22
PspLI CGTACG 1 cut(s) 401
PspN4I GGNNCC 1 cut(s) 247
PspPI GGNCC 1 cut(s) 298
PstI CTGCAG 1 cut(s) 792
PstNI CAGNNNCTG 1 cut(s) 11
RsaI GTAC 2 cut(s) 403, 571
RsaNI GTAC 2 cut(s) 402, 570
SaqAI TTAA 2 cut(s) 507, 804
SatI GCNGC 2 cut(s) 783, 791
Sau3AI GATC 2 cut(s) 430, 559
Sau96I GGNCC 1 cut(s) 298
ScaI AGTACT 1 cut(s) 571
ScrFI CCNGG 1 cut(s) 614
SduI GDGCHC 2 cut(s) 348, 372
SfaNI GCATC 1 cut(s) 652
SfcI CTRYAG 1 cut(s) 788
SinI GGWCC 1 cut(s) 298
SphI GCATGC 1 cut(s) 293
Sse9I AATT 5 cut(s) 198, 336, 455, 698, 805
SsiI CCGC 1 cut(s) 35
SspMI CTAG 1 cut(s) 312
StyD4I CCNGG 1 cut(s) 612
StyI CCWWGG 2 cut(s) 144, 628
TaaI ACNGT 5 cut(s) 350, 406, 536, 625, 760
TaiI ACGT 1 cut(s) 427
TaqI TCGA 5 cut(s) 136, 216, 657, 669, 711
TaqII GACCGA 1 cut(s) 557
TasI AATT 5 cut(s) 198, 336, 455, 698, 805
TatI WGTACW 1 cut(s) 569
TfiI GAWTC 2 cut(s) 495, 584
Tru1I TTAA 2 cut(s) 507, 804
Tru9I TTAA 2 cut(s) 507, 804
TseFI GTSAC 1 cut(s) 538
TseI GCWGC 2 cut(s) 782, 790
Tsp45I GTSAC 1 cut(s) 538
TspDTI ATGAA 5 cut(s) 108, 460, 487, 584, 763
TspGWI ACGGA 1 cut(s) 200
VpaK11BI GGWCC 1 cut(s) 298
XceI RCATGY 2 cut(s) 293, 707
XmnI GAANNNNTTC 2 cut(s) 202, 467
XspI CTAG 1 cut(s) 312
ZrmI AGTACT 1 cut(s) 571
Zsp2I ATGCAT 1 cut(s) 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.