Rh2CG086500

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000515
Physical Location & Seq
Reverse (-)
7122975 .. 7125263
2289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG086500.1

Sequence Viewer

Length: 810 bp
ATGGCAGAGTCTGCTCAAAACCCAGTGGCCCAGCAACAGAAAATCATTGTAACCAACAAACATGGTGAAAAGCTTGTGGGCATATTACATGACACTGGTTCTGGGGATATTGTAATCTTATGCCATGGCTTTACATCCACCAAGGACGACCCTATTATGGTGAACCTCGCTGTTGCATTGGAAAGTGAAGGAATTAGTTCATTTCGTTTTGATTTTTCTGGAAATGGGGAAAGTGAAGGCATCTTTGAGTATGGTAACTATCGGAAAGAAGCTGATGACTTACATGCTGTAGTTGAACACTTCTCAGGGACAAGCCGTGTAGCAAGTGTAATTTTAGGGCACAGCAAAGGAGGTGATGTGGTGCTTCTGTATGCTTCTAAGTATCATGACATTCGTACGGTTGTCAATGTTTCTGGACGTTATGATCTGAAGAAAGGCATTGGAGAACGCTTGGGGAAAGACTTTATGGAAATGATCAAGAAGGAAGGCTTCCTTGATATCAAGAATAACACAGGAGGTGTTGCTTACCGTGTGACTGAAGAAAGTCTGATGGATCGCCTAAGTACTGATATGCAAGAATCATGCCTTCAGATTGGCAAAGAATGCCAGGTGTTGACAGTCCATGGATCTGATGATGAGGTCATCCCTGTGGACGATGCAGTGGAGTTTTCCAAGATAATAACTAACCATAAATTACATATTGTTCGAGGAGCTAATCATTGTTACACCTCGCATCAAGCTGAGTTAGCTTCAGTTGTGGTGGACTACATAAAGACCGCTCTGCAGCAGGACAAGACTACTTCCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

269

Amino Acids

29.53

Weight (kDa)

5.42

Isoelectric Point (pI)

19.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 37 - 150 1.8e-12 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 37 - 139 1.3e-09 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47590 AT3G47590
fragaria_vesca FvH4_1g07650 FvH4_1g07670 FvH4_1g07671 FvH4_1g07680 FvH4_1g07690 FvH4_1g07700 FvH4_1g07700
malus_domestica MD02G1080900.v1.1 MD02G1081000.v1.1 MD02G1081300.v1.1 MD02G1081400.v1.1 MD02G1094000.v1.1 MD15G1208700.v1.1
prunus_persica Prupe.7G208200_v2.0.a1 Prupe.7G208200_v2.0.a1
pyrus_communis pycom02g07440 pycom15g18480
rosa_chinensis RchiOBHm_Chr2g0093511 RchiOBHm_Chr2g0093521 RchiOBHm_Chr2g0093531 RchiOBHm_Chr2g0093541 RchiOBHm_Chr2g0093551 RchiOBHm_Chr2g0093561
rosa_laevigata RLG00000016413 RLG00000016414 RLG00000016415 RLG00000016416 RLG00000016417
rosa_multiflora Rmu_sc0004423.1_g000003 Rmu_sc0004423.1_g000004 Rmu_sc0004423.1_g000005 Rmu_sc0004423.1_g000006 Rmu_sc0006964.1_g000003 Rmu_sc0039672.1_g000001 Rmu_sc0039672.1_g000003
rosa_roxburghii Rroxscaffold_2G00147990 Rroxscaffold_2G00148000 Rroxscaffold_2G00148010 Rroxscaffold_2G00148020 Rroxscaffold_4G00313540
rosa_rugosa Rorug01G0140600.1 Rorug01G0140700.1 Rorug02G0037000 Rorug02G0037100 Rorug02G0037200 Rorug02G0037300 Rorug02G0037400 Rorug02G0037500 Rorug02G0037600
rosa_samantha Rh2AG083400 Rh2AG083500 Rh2AG083700 Rh2AG083800 Rh2AG083900 Rh2AG084000 Rh2BG084200 Rh2BG084300 Rh2BG084400 Rh2BG084500 Rh2BG084600 Rh2CG086300 Rh2CG086400 Rh2CG086500 Rh2CG086700 Rh2CG086800 Rh2CG086900 Rh2DG082200 Rh2DG082300 Rh2DG082400 Rh2DG082500 Rh2DG082600 Rh2DG082700
rosa_wichuraiana Rw1G013200 Rw2G006360 Rw2G006370 Rw2G006380 Rw2G006390 Rw2G006400 Rw2G007130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 779
AciI CCGC 1 cut(s) 777
AclWI GGATC 2 cut(s) 561, 634
AcuI CTGAAG 4 cut(s) 449, 558, 572, 735
AfaI GTAC 2 cut(s) 397, 565
AfiI CCNNNNNNNGG 1 cut(s) 157
AgsI TTSAA 1 cut(s) 296
AjnI CCWGG 1 cut(s) 606
AluBI AGCT 5 cut(s) 73, 272, 713, 740, 749
AluI AGCT 5 cut(s) 73, 272, 713, 740, 749
AlwI GGATC 2 cut(s) 561, 634
AlwNI CAGNNNCTG 1 cut(s) 11
AoxI GGCC 1 cut(s) 27
ApeKI GCWGC 1 cut(s) 784
Asp700I GAANNNNTTC 1 cut(s) 196
AspS9I GGNCC 1 cut(s) 28
AsuHPI GGTGA 3 cut(s) 77, 172, 365
BaeGI GKGCMC 1 cut(s) 342
BbvI GCAGC 1 cut(s) 796
BccI CCATC 1 cut(s) 544
BceAI ACGGC 1 cut(s) 300
BciT130I CCWGG 1 cut(s) 608
BclI TGATCA 1 cut(s) 474
BfaI CTAG 1 cut(s) 808
BfmI CTRYAG 2 cut(s) 288, 782
BisI GCNGC 1 cut(s) 785
BlsI GCNGC 1 cut(s) 786
BmcAI AGTACT 1 cut(s) 565
Bme1390I CCNGG 1 cut(s) 608
BmgT120I GGNCC 1 cut(s) 28
BmrFI CCNGG 1 cut(s) 608
BmrI ACTGGG 1 cut(s) 17
BmsI GCATC 3 cut(s) 249, 646, 742
BmuI ACTGGG 1 cut(s) 17
BsaJI CCNNGG 3 cut(s) 124, 141, 622
BsaXI ACNNNNNCTCC 2 cut(s) 342, 372
Bsc4I CCNNNNNNNGG 1 cut(s) 157
Bse1I ACTGG 2 cut(s) 23, 100
BseBI CCWGG 1 cut(s) 608
BseDI CCNNGG 3 cut(s) 124, 141, 622
BseGI GGATG 2 cut(s) 134, 642
BseLI CCNNNNNNNGG 1 cut(s) 157
BseMII CTCAG 2 cut(s) 318, 732
BseNI ACTGG 2 cut(s) 23, 100
BseRI GAGGAG 1 cut(s) 723
BseSI GKGCMC 1 cut(s) 342
BseXI GCAGC 1 cut(s) 796
BseYI CCCAGC 1 cut(s) 30
BshFI GGCC 1 cut(s) 29
BsiWI CGTACG 1 cut(s) 395
BslFI GGGAC 1 cut(s) 322
BslI CCNNNNNNNGG 1 cut(s) 157
BsmFI GGGAC 1 cut(s) 322
BsmI GAATGC 1 cut(s) 608
BsnI GGCC 1 cut(s) 29
Bsp1286I GDGCHC 1 cut(s) 342
Bsp143I GATC 4 cut(s) 424, 474, 553, 626
Bsp19I CCATGG 2 cut(s) 124, 622
BspACI CCGC 1 cut(s) 777
BspANI GGCC 1 cut(s) 29
BspCNI CTCAG 2 cut(s) 317, 733
BspHI TCATGA 1 cut(s) 385
BspMAI CTGCAG 1 cut(s) 786
BspPI GGATC 2 cut(s) 561, 634
BsrBI CCGCTC 1 cut(s) 779
BsrI ACTGG 2 cut(s) 23, 100
BssECI CCNNGG 3 cut(s) 124, 141, 622
BssMI GATC 4 cut(s) 424, 474, 553, 626
BssT1I CCWWGG 3 cut(s) 124, 141, 622
Bst2UI CCWGG 1 cut(s) 608
Bst4CI ACNGT 3 cut(s) 400, 530, 619
BstAPI GCANNNNNTGC 2 cut(s) 11, 603
BstDEI CTNAG 4 cut(s) 304, 378, 560, 741
BstDSI CCRYGG 2 cut(s) 124, 622
BstF5I GGATG 2 cut(s) 134, 642
BstKTI GATC 4 cut(s) 427, 477, 556, 629
BstMBI GATC 4 cut(s) 424, 474, 553, 626
BstMWI GCNNNNNNNGC 3 cut(s) 11, 603, 746
BstNI CCWGG 1 cut(s) 608
BstNSI RCATGY 1 cut(s) 287
BstSCI CCNGG 1 cut(s) 606
BstSFI CTRYAG 2 cut(s) 288, 782
BstSLI GKGCMC 1 cut(s) 342
BstV1I GCAGC 1 cut(s) 796
BstX2I RGATCY 1 cut(s) 626
BstYI RGATCY 1 cut(s) 626
BsuRI GGCC 1 cut(s) 29
BtgI CCRYGG 2 cut(s) 124, 622
BtsCI GGATG 2 cut(s) 134, 642
BtsI GCAGTG 1 cut(s) 666
BtsIMutI CAGTG 3 cut(s) 30, 93, 666
CaiI CAGNNNCTG 1 cut(s) 11
CciI TCATGA 1 cut(s) 385
Cfr13I GGNCC 1 cut(s) 28
Csp6I GTAC 2 cut(s) 396, 564
CviAII CATG 7 cut(s) 62, 89, 125, 284, 386, 582, 623
CviJI RGCY 9 cut(s) 29, 73, 129, 272, 315, 489, 713, 740, 749
CviKI_1 RGCY 9 cut(s) 29, 73, 129, 272, 315, 489, 713, 740, 749
CviQI GTAC 2 cut(s) 396, 564
DdeI CTNAG 4 cut(s) 304, 378, 560, 741
DpnI GATC 4 cut(s) 426, 476, 555, 628
DpnII GATC 4 cut(s) 424, 474, 553, 626
Eco130I CCWWGG 3 cut(s) 124, 141, 622
Eco32I GATATC 1 cut(s) 499
Eco57I CTGAAG 4 cut(s) 449, 558, 572, 735
EcoRII CCWGG 1 cut(s) 606
EcoRV GATATC 1 cut(s) 499
EcoT14I CCWWGG 3 cut(s) 124, 141, 622
ErhI CCWWGG 3 cut(s) 124, 141, 622
FaeI CATG 7 cut(s) 65, 92, 128, 287, 389, 585, 626
FalI AAGNNNNNCTT 4 cut(s) 473, 505, 477, 509
FaqI GGGAC 1 cut(s) 322
FatI CATG 7 cut(s) 61, 88, 124, 283, 385, 581, 622
FbaI TGATCA 1 cut(s) 474
Fnu4HI GCNGC 1 cut(s) 785
FokI GGATG 2 cut(s) 121, 629
Fsp4HI GCNGC 1 cut(s) 785
FspBI CTAG 1 cut(s) 808
GluI GCNGC 1 cut(s) 785
GsaI CCCAGC 1 cut(s) 34
HaeIII GGCC 1 cut(s) 29
Hin1II CATG 7 cut(s) 65, 92, 128, 287, 389, 585, 626
HincII GTYRAC 1 cut(s) 615
HindII GTYRAC 1 cut(s) 615
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 2 cut(s) 8, 578
HphI GGTGA 3 cut(s) 77, 172, 365
Hpy166II GTNNAC 4 cut(s) 163, 615, 652, 763
Hpy188I TCNGA 5 cut(s) 264, 429, 549, 591, 631
Hpy188III TCNNGA 5 cut(s) 219, 386, 414, 478, 502
Hpy8I GTNNAC 4 cut(s) 163, 615, 652, 763
HpyAV CCTTC 5 cut(s) 182, 230, 475, 479, 596
HpyCH4III ACNGT 3 cut(s) 400, 530, 619
HpyCH4IV ACGT 1 cut(s) 418
HpyCH4V TGCA 4 cut(s) 176, 574, 659, 784
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 603, 746
HpyF3I CTNAG 4 cut(s) 304, 378, 560, 741
HpySE526I ACGT 1 cut(s) 418
Hsp92II CATG 7 cut(s) 65, 92, 128, 287, 389, 585, 626
Ksp22I TGATCA 1 cut(s) 474
Kzo9I GATC 4 cut(s) 424, 474, 553, 626
LmnI GCTCC 1 cut(s) 710
Lsp1109I GCAGC 1 cut(s) 796
LweI GCATC 3 cut(s) 249, 646, 742
MaeI CTAG 1 cut(s) 808
MaeII ACGT 1 cut(s) 418
MaeIII GTNAC 4 cut(s) 49, 254, 532, 722
MalI GATC 4 cut(s) 426, 476, 555, 628
MbiI CCGCTC 1 cut(s) 779
MboI GATC 4 cut(s) 424, 474, 553, 626
MboII GAAGA 2 cut(s) 442, 551
MflI RGATCY 1 cut(s) 626
MhlI GDGCHC 1 cut(s) 342
MluCI AATT 3 cut(s) 192, 330, 692
MlyI GAGTC 1 cut(s) 17
MnlI CCTC 6 cut(s) 176, 344, 509, 631, 701, 739
MroXI GAANNNNTTC 1 cut(s) 196
MslI CAYNNNNRTG 1 cut(s) 647
MspR9I CCNGG 1 cut(s) 608
Mva1269I GAATGC 1 cut(s) 608
MvaI CCWGG 1 cut(s) 608
MwoI GCNNNNNNNGC 3 cut(s) 11, 603, 746
NcoI CCATGG 2 cut(s) 124, 622
NdeII GATC 4 cut(s) 424, 474, 553, 626
NlaIII CATG 7 cut(s) 65, 92, 128, 287, 389, 585, 626
NmuCI GTSAC 1 cut(s) 532
NspI RCATGY 1 cut(s) 287
PagI TCATGA 1 cut(s) 385
PctI GAATGC 1 cut(s) 608
PdmI GAANNNNTTC 1 cut(s) 196
PfeI GAWTC 1 cut(s) 578
Pfl23II CGTACG 1 cut(s) 395
PkrI GCNGC 1 cut(s) 786
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
Psp6I CCWGG 1 cut(s) 606
PspFI CCCAGC 1 cut(s) 30
PspGI CCWGG 1 cut(s) 606
PspLI CGTACG 1 cut(s) 395
PspPI GGNCC 1 cut(s) 28
PstI CTGCAG 1 cut(s) 786
PstNI CAGNNNCTG 1 cut(s) 11
PsuI RGATCY 1 cut(s) 626
RsaI GTAC 2 cut(s) 397, 565
RsaNI GTAC 2 cut(s) 396, 564
RseI CAYNNNNRTG 1 cut(s) 647
SatI GCNGC 1 cut(s) 785
Sau3AI GATC 4 cut(s) 424, 474, 553, 626
Sau96I GGNCC 1 cut(s) 28
ScaI AGTACT 1 cut(s) 565
SchI GAGTC 1 cut(s) 17
ScrFI CCNGG 1 cut(s) 608
SduI GDGCHC 1 cut(s) 342
SfaNI GCATC 3 cut(s) 249, 646, 742
SfcI CTRYAG 2 cut(s) 288, 782
SmiMI CAYNNNNRTG 1 cut(s) 647
Sse9I AATT 3 cut(s) 192, 330, 692
SsiI CCGC 1 cut(s) 777
SspMI CTAG 1 cut(s) 808
StyD4I CCNGG 1 cut(s) 606
StyI CCWWGG 3 cut(s) 124, 141, 622
TaaI ACNGT 3 cut(s) 400, 530, 619
TaiI ACGT 1 cut(s) 421
TaqI TCGA 1 cut(s) 706
TasI AATT 3 cut(s) 192, 330, 692
TatI WGTACW 1 cut(s) 563
TfiI GAWTC 1 cut(s) 578
TscAI CASTG 3 cut(s) 30, 100, 666
TseFI GTSAC 1 cut(s) 532
TseI GCWGC 1 cut(s) 784
Tsp45I GTSAC 1 cut(s) 532
TspDTI ATGAA 1 cut(s) 189
TspRI CASTG 3 cut(s) 30, 100, 666
XceI RCATGY 1 cut(s) 287
XmnI GAANNNNTTC 1 cut(s) 196
XspI CTAG 1 cut(s) 808
ZrmI AGTACT 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.