RLG00000016417

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000515
Physical Location & Seq
Reverse (-)
6498054 .. 6505226
7173 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016417

Sequence Viewer

Length: 777 bp
ATGGCCCAGACTGCCCAAAACCCAGTGAAGCAGCAACTGAAAATCGTTGTACCCAACAAACATGGTGAAAAACTTGTGGGCTTACTACATGACACAGGTTCTGGGGAGATTATAATCTTATGTCATGCCTTCTCCTTCGGTTTCCAGGAGACAACAAGTATTGCAAACATTGCTGTTGCATTGGAGAATGAAGGAATTAGCTCCTTCCGTTTTGACTTTGCTGGAAATGGGGAAAGTGAAGGTACCTTTCAATATGGTCATTATCGTAGAGAGGCCGATGACTTGCATGCTGTAGTCCAACACTTCTCTGGGGCAAATCGTGTACCAAGTGCAATCCTTGGGCACAGTAAAGGAGGTGATGTTGTGCTCCTATATGCTTCCAAGTATCATGACATTCCTACCGTTGTCAATGTTTCTGGGTGTTATGATCTGAAGAGAGGCCGTAAAGAATGCTTGGGAGAAGACTTTATGCAAACAATCAAGAAGGAAGGATTTATTGATGTTCAGCATCAGCGTGGAAGCATTGATTATCGAGTTACCGAAGAAAGCTTGATGGATCGCTTAAACATCAATATATGCATGGTGTTAACTGTCCACGGGTCTGCTGATGAAATCATCGGTGTTGAAGATGCATTAGAGTTTGACAAGATTATACCTAACCACAAATTACATATCATAGAGGGAGCTAACCATAACTACAGCTCACATCAAGCCGAGCTGGCATCAGTTGTTGTGGACTTCATAAAGACATCTATTCAGCAGGACAAGGCTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

259

Amino Acids

28.49

Weight (kDa)

5.65

Isoelectric Point (pI)

37.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 50 - 143 5.1e-11 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47590 AT3G47590
fragaria_vesca FvH4_1g07650 FvH4_1g07670 FvH4_1g07671 FvH4_1g07680 FvH4_1g07690 FvH4_1g07700 FvH4_1g07700
malus_domestica MD02G1080900.v1.1 MD02G1081000.v1.1 MD02G1081300.v1.1 MD02G1081400.v1.1 MD02G1094000.v1.1 MD15G1208700.v1.1
prunus_persica Prupe.7G208200_v2.0.a1 Prupe.7G208200_v2.0.a1
pyrus_communis pycom02g07440 pycom15g18480
rosa_chinensis RchiOBHm_Chr2g0093511 RchiOBHm_Chr2g0093521 RchiOBHm_Chr2g0093531 RchiOBHm_Chr2g0093541 RchiOBHm_Chr2g0093551 RchiOBHm_Chr2g0093561
rosa_laevigata RLG00000016413 RLG00000016414 RLG00000016415 RLG00000016416 RLG00000016417
rosa_multiflora Rmu_sc0004423.1_g000003 Rmu_sc0004423.1_g000004 Rmu_sc0004423.1_g000005 Rmu_sc0004423.1_g000006 Rmu_sc0006964.1_g000003 Rmu_sc0039672.1_g000001 Rmu_sc0039672.1_g000003
rosa_roxburghii Rroxscaffold_2G00147990 Rroxscaffold_2G00148000 Rroxscaffold_2G00148010 Rroxscaffold_2G00148020 Rroxscaffold_4G00313540
rosa_rugosa Rorug01G0140600.1 Rorug01G0140700.1 Rorug02G0037000 Rorug02G0037100 Rorug02G0037200 Rorug02G0037300 Rorug02G0037400 Rorug02G0037500 Rorug02G0037600
rosa_samantha Rh2AG083400 Rh2AG083500 Rh2AG083700 Rh2AG083800 Rh2AG083900 Rh2AG084000 Rh2BG084200 Rh2BG084300 Rh2BG084400 Rh2BG084500 Rh2BG084600 Rh2CG086300 Rh2CG086400 Rh2CG086500 Rh2CG086700 Rh2CG086800 Rh2CG086900 Rh2DG082200 Rh2DG082300 Rh2DG082400 Rh2DG082500 Rh2DG082600 Rh2DG082700
rosa_wichuraiana Rw1G013200 Rw2G006360 Rw2G006370 Rw2G006380 Rw2G006390 Rw2G006400 Rw2G007130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 113
Acc65I GGTACC 1 cut(s) 242
AccB1I GGYRCC 1 cut(s) 242
AclWI GGATC 1 cut(s) 564
AcuI CTGAAG 1 cut(s) 452
AfaI GTAC 3 cut(s) 51, 244, 324
AgsI TTSAA 2 cut(s) 251, 626
AjnI CCWGG 1 cut(s) 144
AluBI AGCT 5 cut(s) 201, 549, 686, 702, 718
AluI AGCT 5 cut(s) 201, 549, 686, 702, 718
Alw21I GWGCWC 1 cut(s) 369
Alw26I GTCTC 1 cut(s) 143
AlwI GGATC 1 cut(s) 564
AlwNI CAGNNNCTG 2 cut(s) 37, 101
AoxI GGCC 3 cut(s) 3, 273, 439
ApeKI GCWGC 1 cut(s) 31
Asp718I GGTACC 1 cut(s) 242
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 2 cut(s) 77, 368
BaeGI GKGCMC 1 cut(s) 345
BanI GGYRCC 1 cut(s) 242
BbsI GAAGAC 1 cut(s) 468
Bbv12I GWGCWC 1 cut(s) 369
BbvI GCAGC 1 cut(s) 43
BccI CCATC 1 cut(s) 547
BceAI ACGGC 1 cut(s) 426
BciT130I CCWGG 1 cut(s) 146
BcoDI GTCTC 1 cut(s) 143
BfmI CTRYAG 2 cut(s) 291, 697
BglI GCCNNNNNGGC 1 cut(s) 719
BisI GCNGC 1 cut(s) 32
BlsI GCNGC 1 cut(s) 33
Bme1390I CCNGG 1 cut(s) 146
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 244
BmrFI CCNGG 1 cut(s) 146
BmrI ACTGGG 1 cut(s) 17
BmsI GCATC 3 cut(s) 517, 619, 731
BmuI ACTGGG 1 cut(s) 17
BpiI GAAGAC 1 cut(s) 468
BsaBI GATNNNNATC 1 cut(s) 113
BsaJI CCNNGG 2 cut(s) 337, 595
BsaXI ACNNNNNCTCC 2 cut(s) 345, 375
Bse1I ACTGG 1 cut(s) 23
Bse3DI GCAATG 1 cut(s) 168
Bse8I GATNNNNATC 1 cut(s) 113
BseBI CCWGG 1 cut(s) 146
BseDI CCNNGG 2 cut(s) 337, 595
BseJI GATNNNNATC 1 cut(s) 113
BseMI GCAATG 1 cut(s) 168
BseNI ACTGG 1 cut(s) 23
BseSI GKGCMC 1 cut(s) 345
BseXI GCAGC 1 cut(s) 43
BshFI GGCC 3 cut(s) 5, 275, 441
BshNI GGYRCC 1 cut(s) 242
BsiHKAI GWGCWC 1 cut(s) 369
BsmAI GTCTC 1 cut(s) 143
BsmI GAATGC 1 cut(s) 455
BsnI GGCC 3 cut(s) 5, 275, 441
Bsp1286I GDGCHC 2 cut(s) 345, 369
Bsp143I GATC 2 cut(s) 427, 556
BspANI GGCC 3 cut(s) 5, 275, 441
BspHI TCATGA 1 cut(s) 388
BspLI GGNNCC 1 cut(s) 244
BspPI GGATC 1 cut(s) 564
BspT107I GGYRCC 1 cut(s) 242
BsrDI GCAATG 1 cut(s) 168
BsrI ACTGG 1 cut(s) 23
BssECI CCNNGG 2 cut(s) 337, 595
BssMI GATC 2 cut(s) 427, 556
BssT1I CCWWGG 1 cut(s) 337
Bst2UI CCWGG 1 cut(s) 146
Bst4CI ACNGT 3 cut(s) 347, 403, 592
Bst6I CTCTTC 1 cut(s) 428
BstAPI GCANNNNNTGC 1 cut(s) 170
BstC8I GCNNGC 2 cut(s) 288, 720
BstDSI CCRYGG 1 cut(s) 595
BstKTI GATC 2 cut(s) 430, 559
BstMAI GTCTC 1 cut(s) 143
BstMBI GATC 2 cut(s) 427, 556
BstMWI GCNNNNNNNGC 3 cut(s) 11, 170, 719
BstNI CCWGG 1 cut(s) 146
BstNSI RCATGY 1 cut(s) 290
BstSCI CCNGG 1 cut(s) 144
BstSFI CTRYAG 2 cut(s) 291, 697
BstSLI GKGCMC 1 cut(s) 345
BstV1I GCAGC 1 cut(s) 43
BstV2I GAAGAC 1 cut(s) 468
BsuRI GGCC 3 cut(s) 5, 275, 441
BtgI CCRYGG 1 cut(s) 595
BtsIMutI CAGTG 1 cut(s) 30
Cac8I GCNNGC 2 cut(s) 288, 720
CaiI CAGNNNCTG 2 cut(s) 37, 101
CciI TCATGA 1 cut(s) 388
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 3 cut(s) 50, 243, 323
CviAII CATG 6 cut(s) 62, 89, 125, 287, 389, 580
CviQI GTAC 3 cut(s) 50, 243, 323
DpnI GATC 2 cut(s) 429, 558
DpnII GATC 2 cut(s) 427, 556
Eam1104I CTCTTC 1 cut(s) 428
EarI CTCTTC 1 cut(s) 428
Eco130I CCWWGG 1 cut(s) 337
Eco57I CTGAAG 1 cut(s) 452
EcoRII CCWGG 1 cut(s) 144
EcoT14I CCWWGG 1 cut(s) 337
EcoT22I ATGCAT 2 cut(s) 581, 634
ErhI CCWWGG 1 cut(s) 337
FaeI CATG 6 cut(s) 65, 92, 128, 290, 392, 583
FatI CATG 6 cut(s) 61, 88, 124, 286, 388, 579
Fnu4HI GCNGC 1 cut(s) 32
Fsp4HI GCNGC 1 cut(s) 32
GluI GCNGC 1 cut(s) 32
HaeIII GGCC 3 cut(s) 5, 275, 441
Hin1II CATG 6 cut(s) 65, 92, 128, 290, 392, 583
HincII GTYRAC 1 cut(s) 588
HindII GTYRAC 1 cut(s) 588
HindIII AAGCTT 1 cut(s) 547
HpaI GTTAAC 1 cut(s) 588
HphI GGTGA 2 cut(s) 77, 368
Hpy166II GTNNAC 4 cut(s) 323, 588, 595, 736
Hpy188I TCNGA 1 cut(s) 432
Hpy188III TCNNGA 2 cut(s) 389, 481
Hpy8I GTNNAC 4 cut(s) 323, 588, 595, 736
HpyAV CCTTC 7 cut(s) 139, 145, 185, 214, 233, 478, 482
HpyCH4III ACNGT 3 cut(s) 347, 403, 592
HpyCH4V TGCA 7 cut(s) 164, 179, 286, 332, 472, 579, 632
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 170, 719
Hsp92II CATG 6 cut(s) 65, 92, 128, 290, 392, 583
KpnI GGTACC 1 cut(s) 246
KspAI GTTAAC 1 cut(s) 588
Kzo9I GATC 2 cut(s) 427, 556
LmnI GCTCC 3 cut(s) 206, 372, 683
Lsp1109I GCAGC 1 cut(s) 43
LweI GCATC 3 cut(s) 517, 619, 731
MaeIII GTNAC 1 cut(s) 535
MalI GATC 2 cut(s) 429, 558
MboI GATC 2 cut(s) 427, 556
MboII GAAGA 4 cut(s) 445, 473, 554, 638
MhlI GDGCHC 2 cut(s) 345, 369
MluCI AATT 2 cut(s) 195, 665
MmeI TCCRAC 1 cut(s) 322
MnlI CCTC 4 cut(s) 265, 347, 431, 673
Mph1103I ATGCAT 2 cut(s) 581, 634
MseI TTAA 2 cut(s) 563, 587
MslI CAYNNNNRTG 1 cut(s) 513
MspR9I CCNGG 1 cut(s) 146
Mva1269I GAATGC 1 cut(s) 455
MvaI CCWGG 1 cut(s) 146
MwoI GCNNNNNNNGC 3 cut(s) 11, 170, 719
NdeII GATC 2 cut(s) 427, 556
NlaIII CATG 6 cut(s) 65, 92, 128, 290, 392, 583
NlaIV GGNNCC 1 cut(s) 244
NmeAIII GCCGAG 1 cut(s) 739
NsiI ATGCAT 2 cut(s) 581, 634
NspI RCATGY 1 cut(s) 290
PaeI GCATGC 1 cut(s) 290
PagI TCATGA 1 cut(s) 388
PctI GAATGC 1 cut(s) 455
PfoI TCCNGGA 1 cut(s) 144
PkrI GCNGC 1 cut(s) 33
PsiI TTATAA 1 cut(s) 113
Psp6I CCWGG 1 cut(s) 144
PspGI CCWGG 1 cut(s) 144
PspN4I GGNNCC 1 cut(s) 244
PspPI GGNCC 1 cut(s) 4
PstNI CAGNNNCTG 2 cut(s) 37, 101
RsaI GTAC 3 cut(s) 51, 244, 324
RsaNI GTAC 3 cut(s) 50, 243, 323
RseI CAYNNNNRTG 1 cut(s) 513
SaqAI TTAA 2 cut(s) 563, 587
SatI GCNGC 1 cut(s) 32
Sau3AI GATC 2 cut(s) 427, 556
Sau96I GGNCC 1 cut(s) 4
ScrFI CCNGG 1 cut(s) 146
SduI GDGCHC 2 cut(s) 345, 369
SfaNI GCATC 3 cut(s) 517, 619, 731
SfcI CTRYAG 2 cut(s) 291, 697
SmiMI CAYNNNNRTG 1 cut(s) 513
SphI GCATGC 1 cut(s) 290
Sse9I AATT 2 cut(s) 195, 665
StyD4I CCNGG 1 cut(s) 144
StyI CCWWGG 1 cut(s) 337
TaaI ACNGT 3 cut(s) 347, 403, 592
TaqI TCGA 1 cut(s) 532
TasI AATT 2 cut(s) 195, 665
Tru1I TTAA 2 cut(s) 563, 587
Tru9I TTAA 2 cut(s) 563, 587
TscAI CASTG 1 cut(s) 30
TseI GCWGC 1 cut(s) 31
TspDTI ATGAA 3 cut(s) 204, 624, 730
TspGWI ACGGA 1 cut(s) 197
TspRI CASTG 1 cut(s) 30
XceI RCATGY 1 cut(s) 290
XcmI CCANNNNNNNNNTGG 1 cut(s) 305
Zsp2I ATGCAT 2 cut(s) 581, 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.