Rroxscaffold_2G00148010

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000515
Physical Location & Seq
Forward (+)
85684292 .. 85686671
2380 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00148010.1

Sequence Viewer

Length: 921 bp
ATGATTATGTCAGCAGCTGCCTATAACTTGTATATAAGCGATCCTGTACTAACTTGGGTACCAAAGTCAGCTGTGAAGATCGAGAAGCAGAGACTGTGCAAAACGAATCTTGGAATAACATATATGGCAGAGGCTGCACAGATGCTAGTGAGCCAGAAGCAGAAAATGATTGTAACCAACAATCATGGTGAAAAGCTTGTCGGCTTATTACATGAAACCGGTTCAAGGGAGATTGTGATCTTGTGTCATGGAGCCGGAGCCTCCAAGGACCACTTTATTATAGAGAACCTTGCTGTTGCATTGGAAAAGCAAGGAATTAGTTCCTTCCGTTTTGATTTCGCTGGAAATGGAGAAAGTGAAGGCGCCTTTCAGTTGGGTCACCTGAGGAGAGAAGCTGATGACGTGCATGCTGTAATCCAACACTTCTCTGGATCAAACCATGTAGTTAGTGCAATTTTCGGGCACAGTAAAGGAGGCGCTGATGTGCTCCTGTATGCTTCAAAGTATCATGACATTCGTACGGTTGTCGAAGCTTCTGCACGCTATGACCTAAAGAAAGGCATTGAAAGACACGTGGGGAAAGACTTTATGGAGAAGATCAAAAAGGAAGGATTCATTGATATTGGAAGTCCTAAATTTCGGGTGACTGAGGAAAGCGTGAAGGACATCCTAAGCACTGATATGCATGAATCGTGCCTCAAGATTGACAAAGATTGCCGGGTGCTAACAGTCCATGGAACTGCTGATGAGATCGTTCCTGTTGAAAATGCATTTGAGTTTGCCAAGATCATACCTAACCACAAAATACATATTATAGAAGGAGCTGATCATTGTTACAAATCGCATCAAGCCGAGTTAGACACAGTTGTTGTGGACTTCATAAAAGCAGCTCTGCAGCAGGACAAGGCTTCTTCCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

306

Amino Acids

33.87

Weight (kDa)

6.38

Isoelectric Point (pI)

34.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 75 - 228 1.1e-13 Serine aminopeptidase, S33
Abhydrolase_11 PF20408 76 - 159 4e-07 Alpha/beta hydrolase domain
Abhydrolase_1 PF00561 78 - 173 1.3e-07 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 79 - 278 6.8e-07 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47590 AT3G47590
fragaria_vesca FvH4_1g07650 FvH4_1g07670 FvH4_1g07671 FvH4_1g07680 FvH4_1g07690 FvH4_1g07700 FvH4_1g07700
malus_domestica MD02G1080900.v1.1 MD02G1081000.v1.1 MD02G1081300.v1.1 MD02G1081400.v1.1 MD02G1094000.v1.1 MD15G1208700.v1.1
prunus_persica Prupe.7G208200_v2.0.a1 Prupe.7G208200_v2.0.a1
pyrus_communis pycom02g07440 pycom15g18480
rosa_chinensis RchiOBHm_Chr2g0093511 RchiOBHm_Chr2g0093521 RchiOBHm_Chr2g0093531 RchiOBHm_Chr2g0093541 RchiOBHm_Chr2g0093551 RchiOBHm_Chr2g0093561
rosa_laevigata RLG00000016413 RLG00000016414 RLG00000016415 RLG00000016416 RLG00000016417
rosa_multiflora Rmu_sc0004423.1_g000003 Rmu_sc0004423.1_g000004 Rmu_sc0004423.1_g000005 Rmu_sc0004423.1_g000006 Rmu_sc0006964.1_g000003 Rmu_sc0039672.1_g000001 Rmu_sc0039672.1_g000003
rosa_roxburghii Rroxscaffold_2G00147990 Rroxscaffold_2G00148000 Rroxscaffold_2G00148010 Rroxscaffold_2G00148020 Rroxscaffold_4G00313540
rosa_rugosa Rorug01G0140600.1 Rorug01G0140700.1 Rorug02G0037000 Rorug02G0037100 Rorug02G0037200 Rorug02G0037300 Rorug02G0037400 Rorug02G0037500 Rorug02G0037600
rosa_samantha Rh2AG083400 Rh2AG083500 Rh2AG083700 Rh2AG083800 Rh2AG083900 Rh2AG084000 Rh2BG084200 Rh2BG084300 Rh2BG084400 Rh2BG084500 Rh2BG084600 Rh2CG086300 Rh2CG086400 Rh2CG086500 Rh2CG086700 Rh2CG086800 Rh2CG086900 Rh2DG082200 Rh2DG082300 Rh2DG082400 Rh2DG082500 Rh2DG082600 Rh2DG082700
rosa_wichuraiana Rw1G013200 Rw2G006360 Rw2G006370 Rw2G006380 Rw2G006390 Rw2G006400 Rw2G007130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 58
AccB1I GGYRCC 2 cut(s) 58, 362
AclWI GGATC 2 cut(s) 35, 439
AcsI RAATTY 1 cut(s) 635
AcvI CACGTG 1 cut(s) 574
AcyI GRCGYC 1 cut(s) 363
AfaI GTAC 3 cut(s) 48, 60, 520
AfiI CCNNNNNNNGG 1 cut(s) 225
AflIII ACRYGT 1 cut(s) 571
AgeI ACCGGT 1 cut(s) 218
AgsI TTSAA 4 cut(s) 225, 501, 566, 764
AjiI CACGTC 1 cut(s) 403
AluBI AGCT 7 cut(s) 17, 71, 196, 395, 533, 824, 890
AluI AGCT 7 cut(s) 17, 71, 196, 395, 533, 824, 890
Alw21I GWGCWC 1 cut(s) 489
Alw26I GTCTC 1 cut(s) 85
AlwI GGATC 2 cut(s) 35, 439
AlwNI CAGNNNCTG 3 cut(s) 17, 94, 134
ApeKI GCWGC 5 cut(s) 14, 17, 134, 887, 895
ApoI RAATTY 1 cut(s) 635
AsiGI ACCGGT 1 cut(s) 218
Asp700I GAANNNNTTC 1 cut(s) 319
Asp718I GGTACC 1 cut(s) 58
AspLEI GCGC 2 cut(s) 365, 479
AspS9I GGNCC 1 cut(s) 268
AsuC2I CCSGG 1 cut(s) 719
AsuHPI GGTGA 3 cut(s) 200, 371, 655
AvaII GGWCC 1 cut(s) 268
AxyI CCTNAGG 1 cut(s) 383
BaeGI GKGCMC 1 cut(s) 465
BaeI ACNNNNGTAYC 4 cut(s) 42, 50, 75, 83
BanI GGYRCC 2 cut(s) 58, 362
BbrPI CACGTG 1 cut(s) 574
Bbv12I GWGCWC 1 cut(s) 489
BbvI GCAGC 5 cut(s) 4, 26, 121, 899, 907
BcgI CGANNNNNNTGC 2 cut(s) 518, 552
BclI TGATCA 1 cut(s) 826
BcnI CCSGG 1 cut(s) 719
BcoDI GTCTC 1 cut(s) 85
BfaI CTAG 2 cut(s) 146, 919
BfmI CTRYAG 1 cut(s) 893
BfoI RGCGCY 2 cut(s) 366, 480
BisI GCNGC 5 cut(s) 15, 18, 135, 888, 896
BlsI GCNGC 5 cut(s) 16, 19, 136, 889, 897
Bme1390I CCNGG 1 cut(s) 719
Bme18I GGWCC 1 cut(s) 268
BmgBI CACGTC 1 cut(s) 403
BmgT120I GGNCC 1 cut(s) 268
BmiI GGNNCC 4 cut(s) 60, 253, 259, 364
BmrFI CCNGG 1 cut(s) 719
BmsI GCATC 2 cut(s) 132, 853
Bpu10I CCTNAGC 1 cut(s) 671
BpuEI CTTGAG 1 cut(s) 683
BpuMI CCSGG 1 cut(s) 719
BsaAI YACGTR 1 cut(s) 574
BsaBI GATNNNNATC 1 cut(s) 236
BsaHI GRCGYC 1 cut(s) 363
BsaJI CCNNGG 2 cut(s) 264, 733
BsaWI WCCGGW 1 cut(s) 218
Bsc4I CCNNNNNNNGG 1 cut(s) 225
Bse118I RCCGGY 1 cut(s) 218
Bse21I CCTNAGG 1 cut(s) 383
Bse8I GATNNNNATC 1 cut(s) 236
BseDI CCNNGG 2 cut(s) 264, 733
BseGI GGATG 1 cut(s) 666
BseJI GATNNNNATC 1 cut(s) 236
BseLI CCNNNNNNNGG 1 cut(s) 225
BseMII CTCAG 2 cut(s) 374, 639
BseRI GAGGAG 1 cut(s) 400
BseSI GKGCMC 1 cut(s) 465
BseXI GCAGC 5 cut(s) 4, 26, 121, 899, 907
BsgI GTGCAG 2 cut(s) 120, 522
BshNI GGYRCC 2 cut(s) 58, 362
BshTI ACCGGT 1 cut(s) 218
BsiHKAI GWGCWC 1 cut(s) 489
BsiSI CCGG 3 cut(s) 219, 255, 718
BsiWI CGTACG 1 cut(s) 518
BslI CCNNNNNNNGG 1 cut(s) 225
BsmAI GTCTC 1 cut(s) 85
Bsp1286I GDGCHC 2 cut(s) 465, 489
Bsp143I GATC 8 cut(s) 40, 78, 237, 431, 597, 750, 786, 826
Bsp19I CCATGG 1 cut(s) 733
BspCNI CTCAG 2 cut(s) 375, 640
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 4 cut(s) 60, 253, 259, 364
BspMAI CTGCAG 1 cut(s) 897
BspPI GGATC 2 cut(s) 35, 439
BspT107I GGYRCC 2 cut(s) 58, 362
BsrFI RCCGGY 1 cut(s) 218
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 2 cut(s) 264, 733
BssMI GATC 8 cut(s) 40, 78, 237, 431, 597, 750, 786, 826
BssNI GRCGYC 1 cut(s) 363
BssT1I CCWWGG 2 cut(s) 264, 733
Bst4CI ACNGT 5 cut(s) 96, 467, 523, 730, 865
BstACI GRCGYC 1 cut(s) 363
BstAPI GCANNNNNTGC 1 cut(s) 134
BstBAI YACGTR 1 cut(s) 574
BstC8I GCNNGC 2 cut(s) 408, 541
BstDEI CTNAG 3 cut(s) 383, 648, 671
BstDSI CCRYGG 1 cut(s) 733
BstEII GGTNACC 1 cut(s) 377
BstF5I GGATG 1 cut(s) 666
BstH2I RGCGCY 2 cut(s) 366, 480
BstHHI GCGC 2 cut(s) 365, 479
BstKTI GATC 8 cut(s) 43, 81, 240, 434, 600, 753, 789, 829
BstMAI GTCTC 1 cut(s) 85
BstMBI GATC 8 cut(s) 40, 78, 237, 431, 597, 750, 786, 826
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstNSI RCATGY 1 cut(s) 410
BstPI GGTNACC 1 cut(s) 377
BstSCI CCNGG 1 cut(s) 717
BstSFI CTRYAG 1 cut(s) 893
BstSLI GKGCMC 1 cut(s) 465
BstV1I GCAGC 5 cut(s) 4, 26, 121, 899, 907
Bsu36I CCTNAGG 1 cut(s) 383
BtgI CCRYGG 1 cut(s) 733
BtrI CACGTC 1 cut(s) 403
BtsCI GGATG 1 cut(s) 666
BtsIMutI CAGTG 1 cut(s) 675
Cac8I GCNNGC 2 cut(s) 408, 541
CaiI CAGNNNCTG 3 cut(s) 17, 94, 134
CciI TCATGA 1 cut(s) 508
CfoI GCGC 2 cut(s) 365, 479
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 1 cut(s) 268
Csp6I GTAC 3 cut(s) 47, 59, 519
CspAI ACCGGT 1 cut(s) 218
CviAII CATG 8 cut(s) 185, 212, 248, 407, 440, 509, 686, 734
CviQI GTAC 3 cut(s) 47, 59, 519
DdeI CTNAG 3 cut(s) 383, 648, 671
DinI GGCGCC 1 cut(s) 364
DpnI GATC 8 cut(s) 42, 80, 239, 433, 599, 752, 788, 828
DpnII GATC 8 cut(s) 40, 78, 237, 431, 597, 750, 786, 826
Eco130I CCWWGG 2 cut(s) 264, 733
Eco47I GGWCC 1 cut(s) 268
Eco72I CACGTG 1 cut(s) 574
Eco81I CCTNAGG 1 cut(s) 383
Eco91I GGTNACC 1 cut(s) 377
EcoO65I GGTNACC 1 cut(s) 377
EcoT14I CCWWGG 2 cut(s) 264, 733
EcoT22I ATGCAT 2 cut(s) 687, 772
EgeI GGCGCC 1 cut(s) 364
EheI GGCGCC 1 cut(s) 364
ErhI CCWWGG 2 cut(s) 264, 733
FaeI CATG 8 cut(s) 188, 215, 251, 410, 443, 512, 689, 737
FalI AAGNNNNNCTT 2 cut(s) 257, 289
FatI CATG 8 cut(s) 184, 211, 247, 406, 439, 508, 685, 733
FbaI TGATCA 1 cut(s) 826
Fnu4HI GCNGC 5 cut(s) 15, 18, 135, 888, 896
FokI GGATG 1 cut(s) 653
Fsp4HI GCNGC 5 cut(s) 15, 18, 135, 888, 896
FspBI CTAG 2 cut(s) 146, 919
GlaI GCGC 2 cut(s) 364, 478
GluI GCNGC 5 cut(s) 15, 18, 135, 888, 896
HaeII RGCGCY 2 cut(s) 366, 480
HapII CCGG 3 cut(s) 219, 255, 718
HhaI GCGC 2 cut(s) 365, 479
Hin1I GRCGYC 1 cut(s) 363
Hin1II CATG 8 cut(s) 188, 215, 251, 410, 443, 512, 689, 737
Hin6I GCGC 2 cut(s) 363, 477
HinP1I GCGC 2 cut(s) 363, 477
HindIII AAGCTT 2 cut(s) 194, 531
HinfI GANTC 3 cut(s) 106, 612, 689
HpaII CCGG 3 cut(s) 219, 255, 718
HphI GGTGA 3 cut(s) 200, 371, 655
Hpy166II GTNNAC 1 cut(s) 874
Hpy188III TCNNGA 4 cut(s) 82, 429, 509, 700
Hpy8I GTNNAC 1 cut(s) 874
HpyAV CCTTC 5 cut(s) 334, 353, 602, 655, 812
HpyCH4III ACNGT 5 cut(s) 96, 467, 523, 730, 865
HpyCH4IV ACGT 2 cut(s) 402, 573
HpyCH4V TGCA 9 cut(s) 99, 137, 299, 406, 452, 539, 685, 770, 895
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpyF3I CTNAG 3 cut(s) 383, 648, 671
HpySE526I ACGT 2 cut(s) 402, 573
Hsp92I GRCGYC 1 cut(s) 363
Hsp92II CATG 8 cut(s) 188, 215, 251, 410, 443, 512, 689, 737
HspAI GCGC 2 cut(s) 363, 477
KasI GGCGCC 1 cut(s) 362
KpnI GGTACC 1 cut(s) 62
Ksp22I TGATCA 1 cut(s) 826
Kzo9I GATC 8 cut(s) 40, 78, 237, 431, 597, 750, 786, 826
LmnI GCTCC 4 cut(s) 251, 257, 492, 821
Lsp1109I GCAGC 5 cut(s) 4, 26, 121, 899, 907
LweI GCATC 2 cut(s) 132, 853
MaeI CTAG 2 cut(s) 146, 919
MaeII ACGT 2 cut(s) 402, 573
MaeIII GTNAC 4 cut(s) 172, 377, 643, 833
MalI GATC 8 cut(s) 42, 80, 239, 433, 599, 752, 788, 828
MboI GATC 8 cut(s) 40, 78, 237, 431, 597, 750, 786, 826
MboII GAAGA 3 cut(s) 88, 607, 903
MhlI GDGCHC 2 cut(s) 465, 489
MluCI AATT 3 cut(s) 315, 453, 635
Mly113I GGCGCC 1 cut(s) 363
MmeI TCCRAC 1 cut(s) 442
MnlI CCTC 6 cut(s) 124, 271, 378, 467, 643, 707
Mph1103I ATGCAT 2 cut(s) 687, 772
MroXI GAANNNNTTC 1 cut(s) 319
MslI CAYNNNNRTG 1 cut(s) 680
MspA1I CMGCKG 2 cut(s) 17, 71
MspI CCGG 3 cut(s) 219, 255, 718
MspR9I CCNGG 1 cut(s) 719
MwoI GCNNNNNNNGC 1 cut(s) 134
NarI GGCGCC 1 cut(s) 363
NciI CCSGG 1 cut(s) 719
NcoI CCATGG 1 cut(s) 733
NdeII GATC 8 cut(s) 40, 78, 237, 431, 597, 750, 786, 826
NlaIII CATG 8 cut(s) 188, 215, 251, 410, 443, 512, 689, 737
NlaIV GGNNCC 4 cut(s) 60, 253, 259, 364
NmeAIII GCCGAG 1 cut(s) 877
NmuCI GTSAC 2 cut(s) 377, 643
NsiI ATGCAT 2 cut(s) 687, 772
NspI RCATGY 1 cut(s) 410
PaeI GCATGC 1 cut(s) 410
PagI TCATGA 1 cut(s) 508
PdmI GAANNNNTTC 1 cut(s) 319
PfeI GAWTC 3 cut(s) 106, 612, 689
Pfl23II CGTACG 1 cut(s) 518
PinAI ACCGGT 1 cut(s) 218
PkrI GCNGC 5 cut(s) 16, 19, 136, 889, 897
PluTI GGCGCC 1 cut(s) 366
PmaCI CACGTG 1 cut(s) 574
PmlI CACGTG 1 cut(s) 574
Ppu21I YACGTR 1 cut(s) 574
PspCI CACGTG 1 cut(s) 574
PspEI GGTNACC 1 cut(s) 377
PspLI CGTACG 1 cut(s) 518
PspN4I GGNNCC 4 cut(s) 60, 253, 259, 364
PspPI GGNCC 1 cut(s) 268
PstI CTGCAG 1 cut(s) 897
PstNI CAGNNNCTG 3 cut(s) 17, 94, 134
PvuII CAGCTG 2 cut(s) 17, 71
RsaI GTAC 3 cut(s) 48, 60, 520
RsaNI GTAC 3 cut(s) 47, 59, 519
RseI CAYNNNNRTG 1 cut(s) 680
SatI GCNGC 5 cut(s) 15, 18, 135, 888, 896
Sau3AI GATC 8 cut(s) 40, 78, 237, 431, 597, 750, 786, 826
Sau96I GGNCC 1 cut(s) 268
ScrFI CCNGG 1 cut(s) 719
SduI GDGCHC 2 cut(s) 465, 489
SfaNI GCATC 2 cut(s) 132, 853
SfcI CTRYAG 1 cut(s) 893
SfoI GGCGCC 1 cut(s) 364
SinI GGWCC 1 cut(s) 268
SmiMI CAYNNNNRTG 1 cut(s) 680
SmlI CTYRAG 1 cut(s) 698
SmoI CTYRAG 1 cut(s) 698
SphI GCATGC 1 cut(s) 410
Sse9I AATT 3 cut(s) 315, 453, 635
SspDI GGCGCC 1 cut(s) 362
SspMI CTAG 2 cut(s) 146, 919
StyD4I CCNGG 1 cut(s) 717
StyI CCWWGG 2 cut(s) 264, 733
TaaI ACNGT 5 cut(s) 96, 467, 523, 730, 865
TaiI ACGT 2 cut(s) 405, 576
TaqI TCGA 2 cut(s) 81, 528
TasI AATT 3 cut(s) 315, 453, 635
TatI WGTACW 1 cut(s) 46
TfiI GAWTC 3 cut(s) 106, 612, 689
TscAI CASTG 1 cut(s) 682
TseFI GTSAC 2 cut(s) 377, 643
TseI GCWGC 5 cut(s) 14, 17, 134, 887, 895
Tsp45I GTSAC 2 cut(s) 377, 643
TspDTI ATGAA 4 cut(s) 228, 604, 702, 868
TspGWI ACGGA 1 cut(s) 317
TspRI CASTG 1 cut(s) 682
VpaK11BI GGWCC 1 cut(s) 268
XapI RAATTY 1 cut(s) 635
XceI RCATGY 1 cut(s) 410
XcmI CCANNNNNNNNNTGG 1 cut(s) 425
XmnI GAANNNNTTC 1 cut(s) 319
XspI CTAG 2 cut(s) 146, 919
Zsp2I ATGCAT 2 cut(s) 687, 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.