Rh2AG083400

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000515
Physical Location & Seq
Reverse (-)
7059243 .. 7061408
2166 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG083400.1

Sequence Viewer

Length: 810 bp
ATGACAGAGGCAGCCCAGAAACCAGTGAGCCAGCCAGAGAAAGTAGTAATAACTAACAAACATGGTGAAAAGCTTGTGGGATTATTGCATGAAACCGGTTCTAAAGAGCTTGTGATCTTGTGCCATGGAGCTCGATCCCACAAGGGAGATAATATTATGACTAAACTTGCCCTTGCATTGGAAAAGGAAGGAATTAGTATTTTCCGTTTCGATTTTGCTGGAAATGGAGAAAGTGAAGGCACCTTTCAGTTTGGTCAATACTGGAGAGAGGTTGATGACTTGCATGCTGTAGTCCAACACTTCTCTGGGACAAACCATACAGTAAGTGCAATTCTTGGCCACAGTAAAGGAGGGGATGAGGTGCTTCTGTATGCTTCTAAGTATGGTGACGTTCGCACGGTTGTCAATGTTTCTGGACGTTATGATCTGAATGGAGGCCTTGAGCTACGGTTAGGGAAAAACTTTATGGAAAGGATCAAGAAGCAAGGGTACATGGATATTAAGGACAAGACAGGAACTTTCGATTGTCGTGTGACGGAGGAAAGTTTGCTGGACCGCATGAATACTAAAATGCATGAATCATGCCTTAAGATTGCCAAAGATTGCCGTGTTTTGACAATCCATGGATCTGATGACGACGTGATCCCCGTTGGAGATGCACTGGAGTTTGCCAAGATAATACCTAACCACAAGTTACATGTTATACAAGGAGCTGATCATTGTTACAGCGATCACCAAGCTGAGTTAGCGTCAACTATTTTGGAATTCCTTAAGGCAGCTTTCCAAGAGGACAAGACTACTTCCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

269

Amino Acids

29.86

Weight (kDa)

6.05

Isoelectric Point (pI)

25.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 35 - 159 1.4e-13 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47590 AT3G47590
fragaria_vesca FvH4_1g07650 FvH4_1g07670 FvH4_1g07671 FvH4_1g07680 FvH4_1g07690 FvH4_1g07700 FvH4_1g07700
malus_domestica MD02G1080900.v1.1 MD02G1081000.v1.1 MD02G1081300.v1.1 MD02G1081400.v1.1 MD02G1094000.v1.1 MD15G1208700.v1.1
prunus_persica Prupe.7G208200_v2.0.a1 Prupe.7G208200_v2.0.a1
pyrus_communis pycom02g07440 pycom15g18480
rosa_chinensis RchiOBHm_Chr2g0093511 RchiOBHm_Chr2g0093521 RchiOBHm_Chr2g0093531 RchiOBHm_Chr2g0093541 RchiOBHm_Chr2g0093551 RchiOBHm_Chr2g0093561
rosa_laevigata RLG00000016413 RLG00000016414 RLG00000016415 RLG00000016416 RLG00000016417
rosa_multiflora Rmu_sc0004423.1_g000003 Rmu_sc0004423.1_g000004 Rmu_sc0004423.1_g000005 Rmu_sc0004423.1_g000006 Rmu_sc0006964.1_g000003 Rmu_sc0039672.1_g000001 Rmu_sc0039672.1_g000003
rosa_roxburghii Rroxscaffold_2G00147990 Rroxscaffold_2G00148000 Rroxscaffold_2G00148010 Rroxscaffold_2G00148020 Rroxscaffold_4G00313540
rosa_rugosa Rorug01G0140600.1 Rorug01G0140700.1 Rorug02G0037000 Rorug02G0037100 Rorug02G0037200 Rorug02G0037300 Rorug02G0037400 Rorug02G0037500 Rorug02G0037600
rosa_samantha Rh2AG083400 Rh2AG083500 Rh2AG083700 Rh2AG083800 Rh2AG083900 Rh2AG084000 Rh2BG084200 Rh2BG084300 Rh2BG084400 Rh2BG084500 Rh2BG084600 Rh2CG086300 Rh2CG086400 Rh2CG086500 Rh2CG086700 Rh2CG086800 Rh2CG086900 Rh2DG082200 Rh2DG082300 Rh2DG082400 Rh2DG082500 Rh2DG082600 Rh2DG082700
rosa_wichuraiana Rw1G013200 Rw2G006360 Rw2G006370 Rw2G006380 Rw2G006390 Rw2G006400 Rw2G007130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 239
AciI CCGC 1 cut(s) 556
AclWI GGATC 4 cut(s) 129, 482, 634, 637
AcoI YGGCCR 1 cut(s) 337
AcsI RAATTY 1 cut(s) 764
AfaI GTAC 1 cut(s) 491
AfiI CCNNNNNNNGG 1 cut(s) 178
AflII CTTAAG 2 cut(s) 587, 770
AflIII ACRYGT 1 cut(s) 697
AgeI ACCGGT 1 cut(s) 95
AjiI CACGTC 1 cut(s) 640
AluBI AGCT 7 cut(s) 73, 109, 131, 445, 713, 740, 779
AluI AGCT 7 cut(s) 73, 109, 131, 445, 713, 740, 779
Alw21I GWGCWC 1 cut(s) 133
AlwI GGATC 4 cut(s) 129, 482, 634, 637
AoxI GGCC 2 cut(s) 337, 436
ApeKI GCWGC 2 cut(s) 11, 776
ApoI RAATTY 1 cut(s) 764
AsiGI ACCGGT 1 cut(s) 95
AspS9I GGNCC 1 cut(s) 553
AsuHPI GGTGA 3 cut(s) 77, 398, 725
AvaII GGWCC 1 cut(s) 553
BalI TGGCCA 1 cut(s) 339
BanI GGYRCC 1 cut(s) 239
BanII GRGCYC 1 cut(s) 133
BarI GAAGNNNNNNTAC 2 cut(s) 473, 505
Bbv12I GWGCWC 1 cut(s) 133
BbvI GCAGC 2 cut(s) 23, 788
BceAI ACGGC 1 cut(s) 591
BclI TGATCA 1 cut(s) 715
BfaI CTAG 1 cut(s) 808
BfmI CTRYAG 1 cut(s) 288
BfrI CTTAAG 2 cut(s) 587, 770
BisI GCNGC 2 cut(s) 12, 777
BlsI GCNGC 2 cut(s) 13, 778
Bme18I GGWCC 1 cut(s) 553
BmgBI CACGTC 1 cut(s) 640
BmgT120I GGNCC 1 cut(s) 553
BmiI GGNNCC 1 cut(s) 241
BmsI GCATC 1 cut(s) 646
BpmI CTGGAG 2 cut(s) 283, 683
BpuEI CTTGAG 1 cut(s) 461
BsaJI CCNNGG 2 cut(s) 124, 622
BsaWI WCCGGW 1 cut(s) 95
Bsc4I CCNNNNNNNGG 1 cut(s) 178
Bse118I RCCGGY 1 cut(s) 95
Bse1I ACTGG 3 cut(s) 23, 266, 666
BseDI CCNNGG 2 cut(s) 124, 622
BseGI GGATG 1 cut(s) 361
BseLI CCNNNNNNNGG 1 cut(s) 178
BseMII CTCAG 1 cut(s) 732
BseNI ACTGG 3 cut(s) 23, 266, 666
BseXI GCAGC 2 cut(s) 23, 788
BshFI GGCC 2 cut(s) 339, 438
BshNI GGYRCC 1 cut(s) 239
BshTI ACCGGT 1 cut(s) 95
BsiHKAI GWGCWC 1 cut(s) 133
BsiSI CCGG 1 cut(s) 96
BslFI GGGAC 1 cut(s) 322
BslI CCNNNNNNNGG 1 cut(s) 178
BsmFI GGGAC 1 cut(s) 322
BsnI GGCC 2 cut(s) 339, 438
Bsp1286I GDGCHC 1 cut(s) 133
Bsp143I GATC 8 cut(s) 114, 134, 424, 474, 626, 642, 715, 730
Bsp19I CCATGG 2 cut(s) 124, 622
BspACI CCGC 1 cut(s) 556
BspANI GGCC 2 cut(s) 339, 438
BspCNI CTCAG 1 cut(s) 733
BspLI GGNNCC 1 cut(s) 241
BspPI GGATC 4 cut(s) 129, 482, 634, 637
BspT107I GGYRCC 1 cut(s) 239
BspTI CTTAAG 2 cut(s) 587, 770
BsrFI RCCGGY 1 cut(s) 95
BsrI ACTGG 3 cut(s) 23, 266, 666
BssAI RCCGGY 1 cut(s) 95
BssECI CCNNGG 2 cut(s) 124, 622
BssMI GATC 8 cut(s) 114, 134, 424, 474, 626, 642, 715, 730
BssT1I CCWWGG 2 cut(s) 124, 622
Bst4CI ACNGT 4 cut(s) 322, 344, 400, 450
BstAFI CTTAAG 2 cut(s) 587, 770
BstC8I GCNNGC 2 cut(s) 32, 285
BstDEI CTNAG 2 cut(s) 378, 741
BstDSI CCRYGG 2 cut(s) 124, 622
BstF5I GGATG 1 cut(s) 361
BstKTI GATC 8 cut(s) 117, 137, 427, 477, 629, 645, 718, 733
BstMBI GATC 8 cut(s) 114, 134, 424, 474, 626, 642, 715, 730
BstMWI GCNNNNNNNGC 1 cut(s) 746
BstNSI RCATGY 2 cut(s) 287, 701
BstSFI CTRYAG 1 cut(s) 288
BstV1I GCAGC 2 cut(s) 23, 788
BstX2I RGATCY 1 cut(s) 626
BstYI RGATCY 1 cut(s) 626
BsuRI GGCC 2 cut(s) 339, 438
BtgI CCRYGG 2 cut(s) 124, 622
BtrI CACGTC 1 cut(s) 640
BtsCI GGATG 1 cut(s) 361
BtsIMutI CAGTG 2 cut(s) 30, 659
Cac8I GCNNGC 2 cut(s) 32, 285
Cfr10I RCCGGY 1 cut(s) 95
Cfr13I GGNCC 1 cut(s) 553
CseI GACGC 1 cut(s) 738
Csp6I GTAC 1 cut(s) 490
CspAI ACCGGT 1 cut(s) 95
CviQI GTAC 1 cut(s) 490
DdeI CTNAG 2 cut(s) 378, 741
DpnI GATC 8 cut(s) 116, 136, 426, 476, 628, 644, 717, 732
DpnII GATC 8 cut(s) 114, 134, 424, 474, 626, 642, 715, 730
EaeI YGGCCR 1 cut(s) 337
Ecl136II GAGCTC 1 cut(s) 131
Eco130I CCWWGG 2 cut(s) 124, 622
Eco147I AGGCCT 1 cut(s) 438
Eco24I GRGCYC 1 cut(s) 133
Eco47I GGWCC 1 cut(s) 553
Eco53kI GAGCTC 1 cut(s) 131
EcoICRI GAGCTC 1 cut(s) 131
EcoRI GAATTC 1 cut(s) 764
EcoT14I CCWWGG 2 cut(s) 124, 622
EcoT22I ATGCAT 1 cut(s) 576
EcoT38I GRGCYC 1 cut(s) 133
ErhI CCWWGG 2 cut(s) 124, 622
FaqI GGGAC 1 cut(s) 322
FbaI TGATCA 1 cut(s) 715
Fnu4HI GCNGC 2 cut(s) 12, 777
FokI GGATG 1 cut(s) 368
FriOI GRGCYC 1 cut(s) 133
Fsp4HI GCNGC 2 cut(s) 12, 777
FspBI CTAG 1 cut(s) 808
GluI GCNGC 2 cut(s) 12, 777
GsuI CTGGAG 2 cut(s) 283, 683
HaeIII GGCC 2 cut(s) 339, 438
HapII CCGG 1 cut(s) 96
HgaI GACGC 1 cut(s) 738
HincII GTYRAC 1 cut(s) 753
HindII GTYRAC 1 cut(s) 753
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 1 cut(s) 578
HpaII CCGG 1 cut(s) 96
HphI GGTGA 3 cut(s) 77, 398, 725
Hpy166II GTNNAC 1 cut(s) 753
Hpy188I TCNGA 2 cut(s) 429, 631
Hpy188III TCNNGA 2 cut(s) 414, 478
Hpy8I GTNNAC 1 cut(s) 753
Hpy99I CGWCG 1 cut(s) 641
HpyAV CCTTC 2 cut(s) 182, 230
HpyCH4III ACNGT 4 cut(s) 322, 344, 400, 450
HpyCH4IV ACGT 3 cut(s) 390, 418, 639
HpyCH4V TGCA 6 cut(s) 88, 176, 283, 329, 574, 659
HpyF10VI GCNNNNNNNGC 1 cut(s) 746
HpyF3I CTNAG 2 cut(s) 378, 741
HpySE526I ACGT 3 cut(s) 390, 418, 639
Ksp22I TGATCA 1 cut(s) 715
Kzo9I GATC 8 cut(s) 114, 134, 424, 474, 626, 642, 715, 730
LmnI GCTCC 2 cut(s) 128, 710
Lsp1109I GCAGC 2 cut(s) 23, 788
LweI GCATC 1 cut(s) 646
MaeI CTAG 1 cut(s) 808
MaeII ACGT 3 cut(s) 390, 418, 639
MaeIII GTNAC 4 cut(s) 386, 532, 693, 722
MalI GATC 8 cut(s) 116, 136, 426, 476, 628, 644, 717, 732
MboI GATC 8 cut(s) 114, 134, 424, 474, 626, 642, 715, 730
MflI RGATCY 1 cut(s) 626
MhlI GDGCHC 1 cut(s) 133
MlsI TGGCCA 1 cut(s) 339
MluCI AATT 3 cut(s) 192, 330, 764
MluNI TGGCCA 1 cut(s) 339
MmeI TCCRAC 2 cut(s) 319, 631
MnlI CCTC 6 cut(s) 262, 344, 352, 428, 532, 781
Mox20I TGGCCA 1 cut(s) 339
Mph1103I ATGCAT 1 cut(s) 576
MscI TGGCCA 1 cut(s) 339
MseI TTAA 3 cut(s) 501, 588, 771
Msp20I TGGCCA 1 cut(s) 339
MspCI CTTAAG 2 cut(s) 587, 770
MspI CCGG 1 cut(s) 96
MwoI GCNNNNNNNGC 1 cut(s) 746
NcoI CCATGG 2 cut(s) 124, 622
NdeII GATC 8 cut(s) 114, 134, 424, 474, 626, 642, 715, 730
NlaIV GGNNCC 1 cut(s) 241
NmuCI GTSAC 2 cut(s) 386, 532
NsiI ATGCAT 1 cut(s) 576
NspI RCATGY 2 cut(s) 287, 701
PaeI GCATGC 1 cut(s) 287
PceI AGGCCT 1 cut(s) 438
PciI ACATGT 1 cut(s) 697
PcsI WCGNNNNNNNCGW 1 cut(s) 645
PfeI GAWTC 1 cut(s) 578
PinAI ACCGGT 1 cut(s) 95
PkrI GCNGC 2 cut(s) 13, 778
PscI ACATGT 1 cut(s) 697
Psp124BI GAGCTC 1 cut(s) 133
PspN4I GGNNCC 1 cut(s) 241
PspPI GGNCC 1 cut(s) 553
PsuI RGATCY 1 cut(s) 626
RsaI GTAC 1 cut(s) 491
RsaNI GTAC 1 cut(s) 490
SacI GAGCTC 1 cut(s) 133
SaqAI TTAA 3 cut(s) 501, 588, 771
SatI GCNGC 2 cut(s) 12, 777
Sau3AI GATC 8 cut(s) 114, 134, 424, 474, 626, 642, 715, 730
Sau96I GGNCC 1 cut(s) 553
SduI GDGCHC 1 cut(s) 133
SfaNI GCATC 1 cut(s) 646
SfcI CTRYAG 1 cut(s) 288
SinI GGWCC 1 cut(s) 553
SmlI CTYRAG 3 cut(s) 440, 587, 770
SmoI CTYRAG 3 cut(s) 440, 587, 770
SphI GCATGC 1 cut(s) 287
Sse9I AATT 3 cut(s) 192, 330, 764
SseBI AGGCCT 1 cut(s) 438
SsiI CCGC 1 cut(s) 556
SspI AATATT 1 cut(s) 154
SspMI CTAG 1 cut(s) 808
SstI GAGCTC 1 cut(s) 133
StuI AGGCCT 1 cut(s) 438
StyI CCWWGG 2 cut(s) 124, 622
TaaI ACNGT 4 cut(s) 322, 344, 400, 450
TaiI ACGT 3 cut(s) 393, 421, 642
TaqI TCGA 3 cut(s) 133, 210, 522
TasI AATT 3 cut(s) 192, 330, 764
TfiI GAWTC 1 cut(s) 578
Tru1I TTAA 3 cut(s) 501, 588, 771
Tru9I TTAA 3 cut(s) 501, 588, 771
TscAI CASTG 2 cut(s) 30, 666
TseFI GTSAC 2 cut(s) 386, 532
TseI GCWGC 2 cut(s) 11, 776
Tsp45I GTSAC 2 cut(s) 386, 532
TspDTI ATGAA 3 cut(s) 105, 575, 591
TspGWI ACGGA 2 cut(s) 194, 551
TspRI CASTG 2 cut(s) 30, 666
Vha464I CTTAAG 2 cut(s) 587, 770
VpaK11BI GGWCC 1 cut(s) 553
XapI RAATTY 1 cut(s) 764
XceI RCATGY 2 cut(s) 287, 701
XcmI CCANNNNNNNNNTGG 1 cut(s) 302
XspI CTAG 1 cut(s) 808
Zsp2I ATGCAT 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.