Rh2BG084600

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000515
Physical Location & Seq
Reverse (-)
6587771 .. 6590046
2276 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG084600.1

Sequence Viewer

Length: 486 bp
ATGGCAGAGTCTGCTCAAAACCCAGGAGGTGATGTGGTGCTGCTGTATGCTTCTAAGTATCATGACATTCGTACTGTTGTCAATGTTTCTGGACGTTATGATCTGAAGAAAGGCATTGGAGAACGCTTGGGGAAAGACTTTATGGAACTGATCAAGAAGGAAGGCTTCCTTGATATCAAGAATAACACAGGAGGTGTTGCTTACCGTGTGACTGAAGAAAGTCTGATGGATCGCCTAAGTACTGATATGCATGAATCATGCCTTCAGATTGGCAAAGAATTCCAGGTGTTGACGGTCCATGGATCTGATGATGAGGTCATCCCTGTGGACGATGCAGTGGAGTTTTCCAAGATAATACCTAACCACAAATTACATATTGTAAGGGGAGCTAACCATAACTACACCTCACATCAAGCCGAGTTGGCTTCAGTTGTGGTGAACTACATAAAGACAGCTCTGCAGCAGGACGAGCCAACTTCCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

161

Amino Acids

17.91

Weight (kDa)

5.44

Isoelectric Point (pI)

29.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47590 AT3G47590
fragaria_vesca FvH4_1g07650 FvH4_1g07670 FvH4_1g07671 FvH4_1g07680 FvH4_1g07690 FvH4_1g07700 FvH4_1g07700
malus_domestica MD02G1080900.v1.1 MD02G1081000.v1.1 MD02G1081300.v1.1 MD02G1081400.v1.1 MD02G1094000.v1.1 MD15G1208700.v1.1
prunus_persica Prupe.7G208200_v2.0.a1 Prupe.7G208200_v2.0.a1
pyrus_communis pycom02g07440 pycom15g18480
rosa_chinensis RchiOBHm_Chr2g0093511 RchiOBHm_Chr2g0093521 RchiOBHm_Chr2g0093531 RchiOBHm_Chr2g0093541 RchiOBHm_Chr2g0093551 RchiOBHm_Chr2g0093561
rosa_laevigata RLG00000016413 RLG00000016414 RLG00000016415 RLG00000016416 RLG00000016417
rosa_multiflora Rmu_sc0004423.1_g000003 Rmu_sc0004423.1_g000004 Rmu_sc0004423.1_g000005 Rmu_sc0004423.1_g000006 Rmu_sc0006964.1_g000003 Rmu_sc0039672.1_g000001 Rmu_sc0039672.1_g000003
rosa_roxburghii Rroxscaffold_2G00147990 Rroxscaffold_2G00148000 Rroxscaffold_2G00148010 Rroxscaffold_2G00148020 Rroxscaffold_4G00313540
rosa_rugosa Rorug01G0140600.1 Rorug01G0140700.1 Rorug02G0037000 Rorug02G0037100 Rorug02G0037200 Rorug02G0037300 Rorug02G0037400 Rorug02G0037500 Rorug02G0037600
rosa_samantha Rh2AG083400 Rh2AG083500 Rh2AG083700 Rh2AG083800 Rh2AG083900 Rh2AG084000 Rh2BG084200 Rh2BG084300 Rh2BG084400 Rh2BG084500 Rh2BG084600 Rh2CG086300 Rh2CG086400 Rh2CG086500 Rh2CG086700 Rh2CG086800 Rh2CG086900 Rh2DG082200 Rh2DG082300 Rh2DG082400 Rh2DG082500 Rh2DG082600 Rh2DG082700
rosa_wichuraiana Rw1G013200 Rw2G006360 Rw2G006370 Rw2G006380 Rw2G006390 Rw2G006400 Rw2G007130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 237, 310
AcsI RAATTY 1 cut(s) 278
AcuI CTGAAG 4 cut(s) 125, 234, 248, 411
AfaI GTAC 2 cut(s) 73, 241
AjnI CCWGG 2 cut(s) 22, 282
AluBI AGCT 2 cut(s) 389, 455
AluI AGCT 2 cut(s) 389, 455
AlwI GGATC 2 cut(s) 237, 310
AlwNI CAGNNNCTG 1 cut(s) 11
ApeKI GCWGC 2 cut(s) 40, 460
ApoI RAATTY 1 cut(s) 278
AspS9I GGNCC 1 cut(s) 295
AsuHPI GGTGA 2 cut(s) 41, 448
AvaII GGWCC 1 cut(s) 295
BbvI GCAGC 2 cut(s) 27, 472
BccI CCATC 1 cut(s) 220
BciT130I CCWGG 2 cut(s) 24, 284
BclI TGATCA 1 cut(s) 150
BfaI CTAG 1 cut(s) 484
BfmI CTRYAG 1 cut(s) 458
BglI GCCNNNNNGGC 1 cut(s) 422
BisI GCNGC 2 cut(s) 41, 461
BlsI GCNGC 2 cut(s) 42, 462
BmcAI AGTACT 1 cut(s) 241
Bme1390I CCNGG 2 cut(s) 24, 284
Bme18I GGWCC 1 cut(s) 295
BmgT120I GGNCC 1 cut(s) 295
BmrFI CCNGG 2 cut(s) 24, 284
BmsI GCATC 1 cut(s) 322
BsaJI CCNNGG 2 cut(s) 22, 298
BsaXI ACNNNNNCTCC 2 cut(s) 18, 48
BseBI CCWGG 2 cut(s) 24, 284
BseDI CCNNGG 2 cut(s) 22, 298
BseGI GGATG 1 cut(s) 318
BseXI GCAGC 2 cut(s) 27, 472
Bsp143I GATC 4 cut(s) 100, 150, 229, 302
Bsp19I CCATGG 1 cut(s) 298
BspHI TCATGA 1 cut(s) 61
BspMAI CTGCAG 1 cut(s) 462
BspPI GGATC 2 cut(s) 237, 310
BssECI CCNNGG 2 cut(s) 22, 298
BssMI GATC 4 cut(s) 100, 150, 229, 302
BssT1I CCWWGG 1 cut(s) 298
Bst2UI CCWGG 2 cut(s) 24, 284
Bst4CI ACNGT 3 cut(s) 76, 206, 295
BstAPI GCANNNNNTGC 1 cut(s) 11
BstDEI CTNAG 2 cut(s) 54, 236
BstDSI CCRYGG 1 cut(s) 298
BstF5I GGATG 1 cut(s) 318
BstKTI GATC 4 cut(s) 103, 153, 232, 305
BstMBI GATC 4 cut(s) 100, 150, 229, 302
BstMWI GCNNNNNNNGC 3 cut(s) 11, 422, 469
BstNI CCWGG 2 cut(s) 24, 284
BstSCI CCNGG 2 cut(s) 22, 282
BstSFI CTRYAG 1 cut(s) 458
BstV1I GCAGC 2 cut(s) 27, 472
BstX2I RGATCY 1 cut(s) 302
BstYI RGATCY 1 cut(s) 302
BtgI CCRYGG 1 cut(s) 298
BtsCI GGATG 1 cut(s) 318
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 1 cut(s) 342
CaiI CAGNNNCTG 1 cut(s) 11
CciI TCATGA 1 cut(s) 61
Cfr13I GGNCC 1 cut(s) 295
Csp6I GTAC 2 cut(s) 72, 240
CviAII CATG 4 cut(s) 62, 251, 258, 299
CviJI RGCY 6 cut(s) 165, 389, 416, 425, 455, 472
CviKI_1 RGCY 6 cut(s) 165, 389, 416, 425, 455, 472
CviQI GTAC 2 cut(s) 72, 240
DdeI CTNAG 2 cut(s) 54, 236
DpnI GATC 4 cut(s) 102, 152, 231, 304
DpnII GATC 4 cut(s) 100, 150, 229, 302
Eco130I CCWWGG 1 cut(s) 298
Eco32I GATATC 1 cut(s) 175
Eco47I GGWCC 1 cut(s) 295
Eco57I CTGAAG 4 cut(s) 125, 234, 248, 411
EcoRI GAATTC 1 cut(s) 278
EcoRII CCWGG 2 cut(s) 22, 282
EcoRV GATATC 1 cut(s) 175
EcoT14I CCWWGG 1 cut(s) 298
EcoT22I ATGCAT 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 298
FaeI CATG 4 cut(s) 65, 254, 261, 302
FalI AAGNNNNNCTT 4 cut(s) 149, 181, 153, 185
FatI CATG 4 cut(s) 61, 250, 257, 298
FbaI TGATCA 1 cut(s) 150
Fnu4HI GCNGC 2 cut(s) 41, 461
FokI GGATG 1 cut(s) 305
Fsp4HI GCNGC 2 cut(s) 41, 461
FspBI CTAG 1 cut(s) 484
GluI GCNGC 2 cut(s) 41, 461
Hin1II CATG 4 cut(s) 65, 254, 261, 302
HincII GTYRAC 1 cut(s) 291
HindII GTYRAC 1 cut(s) 291
HinfI GANTC 2 cut(s) 8, 254
HphI GGTGA 2 cut(s) 41, 448
Hpy166II GTNNAC 3 cut(s) 291, 328, 439
Hpy188I TCNGA 4 cut(s) 105, 225, 267, 307
Hpy188III TCNNGA 4 cut(s) 62, 90, 154, 178
Hpy8I GTNNAC 3 cut(s) 291, 328, 439
HpyAV CCTTC 3 cut(s) 151, 155, 272
HpyCH4III ACNGT 3 cut(s) 76, 206, 295
HpyCH4IV ACGT 1 cut(s) 94
HpyCH4V TGCA 3 cut(s) 250, 335, 460
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 422, 469
HpyF3I CTNAG 2 cut(s) 54, 236
HpySE526I ACGT 1 cut(s) 94
Hsp92II CATG 4 cut(s) 65, 254, 261, 302
Ksp22I TGATCA 1 cut(s) 150
Kzo9I GATC 4 cut(s) 100, 150, 229, 302
LmnI GCTCC 1 cut(s) 386
LpnPI CCDG 8 cut(s) 9, 36, 75, 174, 269, 296, 336, 449
Lsp1109I GCAGC 2 cut(s) 27, 472
LweI GCATC 1 cut(s) 322
MaeI CTAG 1 cut(s) 484
MaeII ACGT 1 cut(s) 94
MaeIII GTNAC 1 cut(s) 208
MalI GATC 4 cut(s) 102, 152, 231, 304
MboI GATC 4 cut(s) 100, 150, 229, 302
MboII GAAGA 2 cut(s) 118, 227
MflI RGATCY 1 cut(s) 302
MluCI AATT 2 cut(s) 278, 368
MlyI GAGTC 1 cut(s) 17
MnlI CCTC 4 cut(s) 20, 185, 307, 415
Mph1103I ATGCAT 1 cut(s) 252
MslI CAYNNNNRTG 1 cut(s) 323
MspR9I CCNGG 2 cut(s) 24, 284
MvaI CCWGG 2 cut(s) 24, 284
MwoI GCNNNNNNNGC 3 cut(s) 11, 422, 469
NcoI CCATGG 1 cut(s) 298
NdeII GATC 4 cut(s) 100, 150, 229, 302
NlaIII CATG 4 cut(s) 65, 254, 261, 302
NmeAIII GCCGAG 1 cut(s) 442
NmuCI GTSAC 1 cut(s) 208
NsiI ATGCAT 1 cut(s) 252
PagI TCATGA 1 cut(s) 61
PfeI GAWTC 1 cut(s) 254
PkrI GCNGC 2 cut(s) 42, 462
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
Psp6I CCWGG 2 cut(s) 22, 282
PspGI CCWGG 2 cut(s) 22, 282
PspPI GGNCC 1 cut(s) 295
PstI CTGCAG 1 cut(s) 462
PstNI CAGNNNCTG 1 cut(s) 11
PsuI RGATCY 1 cut(s) 302
RsaI GTAC 2 cut(s) 73, 241
RsaNI GTAC 2 cut(s) 72, 240
RseI CAYNNNNRTG 1 cut(s) 323
SatI GCNGC 2 cut(s) 41, 461
Sau3AI GATC 4 cut(s) 100, 150, 229, 302
Sau96I GGNCC 1 cut(s) 295
ScaI AGTACT 1 cut(s) 241
SchI GAGTC 1 cut(s) 17
ScrFI CCNGG 2 cut(s) 24, 284
SetI ASST 9 cut(s) 31, 97, 196, 288, 318, 361, 391, 407, 457
SfaNI GCATC 1 cut(s) 322
SfcI CTRYAG 1 cut(s) 458
SinI GGWCC 1 cut(s) 295
SmiMI CAYNNNNRTG 1 cut(s) 323
Sse9I AATT 2 cut(s) 278, 368
SspMI CTAG 1 cut(s) 484
StyD4I CCNGG 2 cut(s) 22, 282
StyI CCWWGG 1 cut(s) 298
TaaI ACNGT 3 cut(s) 76, 206, 295
TaiI ACGT 1 cut(s) 97
TasI AATT 2 cut(s) 278, 368
TatI WGTACW 1 cut(s) 239
TfiI GAWTC 1 cut(s) 254
TscAI CASTG 1 cut(s) 342
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 2 cut(s) 40, 460
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 1 cut(s) 267
TspRI CASTG 1 cut(s) 342
VpaK11BI GGWCC 1 cut(s) 295
XapI RAATTY 1 cut(s) 278
XspI CTAG 1 cut(s) 484
ZrmI AGTACT 1 cut(s) 241
Zsp2I ATGCAT 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.