MD02G1081400.v1.1

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000515
Physical Location & Seq
Reverse (-)
6385734 .. 6388034
2301 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1081400.v1.1.491

Sequence Viewer

Length: 810 bp
ATGGCAGAAGCTGCAAAAAATCCAGTGGGTCAGCAACAGAGAATCATCGTACCCAACAAACACGGTGAAAAGCTTGTGGGATTGTTACACGAAACCAGTTCCCCGGACCTTGTAATCTTATGTCATGGTTTTCGAGCCACCAAGGAAAACTCTATCATGGTGAACCTTGCTGTTGCACTGGAAAATGAAGGGATCAGTTCCTTCCGTTTTGACTTTGCCGGAATTGGGGAAAGTGAAGGCTCCTTTCAGTATGCTAGCTTTCGGAGAGAGGCTGATGACTTGCACTCTGTTGTTGAATACTTCTCTGGGGCAAAACGTGCACCCAGTGCAATTATTGGACACAGTAGAGGAGGCGTTGCTGTGCTTCTGTATGCTTCCATATATCATAACATATGTACGGTTGTCAACGTTTCTGGAGGTTATGATCTGAAGAGAGGCATTGAAGAACGCTGTGGAAAAGACTTTATGGAAGTAATCAAGAAGGAAGGGTTCATTGATGTTAAGATTAAGTCAGGAGATGGTAATTATCGTCTGACCAAGGAGAGTTTGATGGATCGCCTAAGTACTGATATGCACGAATCATGCCTTAAGATTGACAAAGAATGCCGGGTGCTGACAATCCATGGAACTGCCGACGAGATCAGCCCTGTTGAAGATGCACTAGAGTTTGCCAAGATAATACCTAACCACAAATTACATCTAGTAGAAGGCGCTAATCATTTGTACACCTCGCATCAAGCCGAGTTAGCATCGGTCGTCATGGACTTCATTAAGGCAGCTCTGCAGCAGGACAAGGCTACTTCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

270

Amino Acids

29.6

Weight (kDa)

5.95

Isoelectric Point (pI)

39.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 37 - 149 8.7e-13 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 38 - 250 6.5e-07 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47590 AT3G47590
fragaria_vesca FvH4_1g07650 FvH4_1g07670 FvH4_1g07671 FvH4_1g07680 FvH4_1g07690 FvH4_1g07700 FvH4_1g07700
malus_domestica MD02G1080900.v1.1 MD02G1081000.v1.1 MD02G1081300.v1.1 MD02G1081400.v1.1 MD02G1094000.v1.1 MD15G1208700.v1.1
prunus_persica Prupe.7G208200_v2.0.a1 Prupe.7G208200_v2.0.a1
pyrus_communis pycom02g07440 pycom15g18480
rosa_chinensis RchiOBHm_Chr2g0093511 RchiOBHm_Chr2g0093521 RchiOBHm_Chr2g0093531 RchiOBHm_Chr2g0093541 RchiOBHm_Chr2g0093551 RchiOBHm_Chr2g0093561
rosa_laevigata RLG00000016413 RLG00000016414 RLG00000016415 RLG00000016416 RLG00000016417
rosa_multiflora Rmu_sc0004423.1_g000003 Rmu_sc0004423.1_g000004 Rmu_sc0004423.1_g000005 Rmu_sc0004423.1_g000006 Rmu_sc0006964.1_g000003 Rmu_sc0039672.1_g000001 Rmu_sc0039672.1_g000003
rosa_roxburghii Rroxscaffold_2G00147990 Rroxscaffold_2G00148000 Rroxscaffold_2G00148010 Rroxscaffold_2G00148020 Rroxscaffold_4G00313540
rosa_rugosa Rorug01G0140600.1 Rorug01G0140700.1 Rorug02G0037000 Rorug02G0037100 Rorug02G0037200 Rorug02G0037300 Rorug02G0037400 Rorug02G0037500 Rorug02G0037600
rosa_samantha Rh2AG083400 Rh2AG083500 Rh2AG083700 Rh2AG083800 Rh2AG083900 Rh2AG084000 Rh2BG084200 Rh2BG084300 Rh2BG084400 Rh2BG084500 Rh2BG084600 Rh2CG086300 Rh2CG086400 Rh2CG086500 Rh2CG086700 Rh2CG086800 Rh2CG086900 Rh2DG082200 Rh2DG082300 Rh2DG082400 Rh2DG082500 Rh2DG082600 Rh2DG082700
rosa_wichuraiana Rw1G013200 Rw2G006360 Rw2G006370 Rw2G006380 Rw2G006390 Rw2G006400 Rw2G007130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 408
AclWI GGATC 2 cut(s) 200, 561
AcuI CTGAAG 1 cut(s) 449
AdeI CACNNNGTG 1 cut(s) 326
AfaI GTAC 4 cut(s) 51, 397, 565, 725
AfiI CCNNNNNNNGG 1 cut(s) 225
AflII CTTAAG 1 cut(s) 587
AgsI TTSAA 4 cut(s) 296, 443, 653, 804
AluBI AGCT 4 cut(s) 11, 73, 258, 779
AluI AGCT 4 cut(s) 11, 73, 258, 779
Alw21I GWGCWC 1 cut(s) 322
Alw44I GTGCAC 1 cut(s) 318
AlwI GGATC 2 cut(s) 200, 561
AlwNI CAGNNNCTG 1 cut(s) 11
ApaLI GTGCAC 1 cut(s) 318
ApeKI GCWGC 3 cut(s) 11, 776, 784
AspLEI GCGC 1 cut(s) 713
AspS9I GGNCC 1 cut(s) 106
AsuC2I CCSGG 2 cut(s) 104, 608
AsuHPI GGTGA 2 cut(s) 77, 172
AsuNHI GCTAGC 1 cut(s) 254
AvaII GGWCC 1 cut(s) 106
BaeGI GKGCMC 1 cut(s) 322
Bbv12I GWGCWC 1 cut(s) 322
BbvI GCAGC 2 cut(s) 788, 796
BccI CCATC 2 cut(s) 512, 544
BcnI CCSGG 2 cut(s) 104, 608
BfaI CTAG 4 cut(s) 255, 662, 701, 808
BfmI CTRYAG 1 cut(s) 782
BfoI RGCGCY 1 cut(s) 714
BfrI CTTAAG 1 cut(s) 587
BisI GCNGC 3 cut(s) 12, 777, 785
BlsI GCNGC 3 cut(s) 13, 778, 786
BmcAI AGTACT 1 cut(s) 565
Bme1390I CCNGG 2 cut(s) 104, 608
Bme18I GGWCC 1 cut(s) 106
BmgT120I GGNCC 1 cut(s) 106
BmiI GGNNCC 1 cut(s) 241
BmrFI CCNGG 2 cut(s) 104, 608
BmrI ACTGGG 1 cut(s) 318
BmsI GCATC 3 cut(s) 646, 742, 758
BmtI GCTAGC 1 cut(s) 258
BmuI ACTGGG 1 cut(s) 318
BpmI CTGGAG 1 cut(s) 435
BpuMI CCSGG 2 cut(s) 104, 608
BsaJI CCNNGG 4 cut(s) 102, 141, 537, 622
Bsc4I CCNNNNNNNGG 1 cut(s) 225
Bse1I ACTGG 4 cut(s) 23, 96, 183, 324
BseDI CCNNGG 4 cut(s) 102, 141, 537, 622
BseLI CCNNNNNNNGG 1 cut(s) 225
BseNI ACTGG 4 cut(s) 23, 96, 183, 324
BseRI GAGGAG 1 cut(s) 363
BseSI GKGCMC 1 cut(s) 322
BseXI GCAGC 2 cut(s) 788, 796
Bsh1285I CGRYCG 1 cut(s) 756
BsiEI CGRYCG 1 cut(s) 756
BsiHKAI GWGCWC 1 cut(s) 322
BsiSI CCGG 3 cut(s) 104, 219, 607
BslI CCNNNNNNNGG 1 cut(s) 225
BsmI GAATGC 1 cut(s) 608
Bsp1286I GDGCHC 1 cut(s) 322
Bsp1407I TGTACA 1 cut(s) 723
Bsp143I GATC 4 cut(s) 192, 424, 553, 639
Bsp19I CCATGG 1 cut(s) 622
BspLI GGNNCC 1 cut(s) 241
BspMAI CTGCAG 1 cut(s) 786
BspOI GCTAGC 1 cut(s) 258
BspPI GGATC 2 cut(s) 200, 561
BspTI CTTAAG 1 cut(s) 587
BsrGI TGTACA 1 cut(s) 723
BsrI ACTGG 4 cut(s) 23, 96, 183, 324
BssECI CCNNGG 4 cut(s) 102, 141, 537, 622
BssMI GATC 4 cut(s) 192, 424, 553, 639
BssT1I CCWWGG 3 cut(s) 141, 537, 622
Bst4CI ACNGT 3 cut(s) 65, 344, 400
Bst6I CTCTTC 1 cut(s) 425
BstAFI CTTAAG 1 cut(s) 587
BstAPI GCANNNNNTGC 3 cut(s) 11, 317, 326
BstAUI TGTACA 1 cut(s) 723
BstC8I GCNNGC 1 cut(s) 256
BstDEI CTNAG 1 cut(s) 560
BstDSI CCRYGG 1 cut(s) 622
BstH2I RGCGCY 1 cut(s) 714
BstHHI GCGC 1 cut(s) 713
BstKTI GATC 4 cut(s) 195, 427, 556, 642
BstMBI GATC 4 cut(s) 192, 424, 553, 639
BstMCI CGRYCG 1 cut(s) 756
BstMWI GCNNNNNNNGC 4 cut(s) 11, 317, 326, 746
BstSCI CCNGG 2 cut(s) 102, 606
BstSFI CTRYAG 1 cut(s) 782
BstSLI GKGCMC 1 cut(s) 322
BstV1I GCAGC 2 cut(s) 788, 796
BtgI CCRYGG 1 cut(s) 622
BtsIMutI CAGTG 3 cut(s) 30, 176, 331
Cac8I GCNNGC 1 cut(s) 256
CaiI CAGNNNCTG 1 cut(s) 11
CfoI GCGC 1 cut(s) 713
Cfr13I GGNCC 1 cut(s) 106
Csp6I GTAC 4 cut(s) 50, 396, 564, 724
CviAII CATG 5 cut(s) 125, 157, 582, 623, 760
CviQI GTAC 4 cut(s) 50, 396, 564, 724
DdeI CTNAG 1 cut(s) 560
DpnI GATC 4 cut(s) 194, 426, 555, 641
DpnII GATC 4 cut(s) 192, 424, 553, 639
DraIII CACNNNGTG 1 cut(s) 326
Eam1104I CTCTTC 1 cut(s) 425
EarI CTCTTC 1 cut(s) 425
Eco130I CCWWGG 3 cut(s) 141, 537, 622
Eco47I GGWCC 1 cut(s) 106
Eco57I CTGAAG 1 cut(s) 449
EcoT14I CCWWGG 3 cut(s) 141, 537, 622
ErhI CCWWGG 3 cut(s) 141, 537, 622
FaeI CATG 5 cut(s) 128, 160, 585, 626, 763
FatI CATG 5 cut(s) 124, 156, 581, 622, 759
FauNDI CATATG 1 cut(s) 392
Fnu4HI GCNGC 3 cut(s) 12, 777, 785
Fsp4HI GCNGC 3 cut(s) 12, 777, 785
FspBI CTAG 4 cut(s) 255, 662, 701, 808
GlaI GCGC 1 cut(s) 712
GluI GCNGC 3 cut(s) 12, 777, 785
GsuI CTGGAG 1 cut(s) 435
HaeII RGCGCY 1 cut(s) 714
HapII CCGG 3 cut(s) 104, 219, 607
HhaI GCGC 1 cut(s) 713
Hin1II CATG 5 cut(s) 128, 160, 585, 626, 763
Hin6I GCGC 1 cut(s) 711
HinP1I GCGC 1 cut(s) 711
HincII GTYRAC 1 cut(s) 406
HindII GTYRAC 1 cut(s) 406
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 2 cut(s) 42, 578
HpaII CCGG 3 cut(s) 104, 219, 607
HphI GGTGA 2 cut(s) 77, 172
Hpy166II GTNNAC 4 cut(s) 163, 320, 406, 726
Hpy188I TCNGA 3 cut(s) 264, 429, 534
Hpy188III TCNNGA 3 cut(s) 414, 478, 513
Hpy8I GTNNAC 4 cut(s) 163, 320, 406, 726
Hpy99I CGWCG 1 cut(s) 638
HpyAV CCTTC 6 cut(s) 182, 211, 230, 475, 479, 701
HpyCH4III ACNGT 3 cut(s) 65, 344, 400
HpyCH4IV ACGT 2 cut(s) 316, 408
HpyCH4V TGCA 8 cut(s) 14, 176, 283, 320, 329, 574, 659, 784
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 317, 326, 746
HpyF3I CTNAG 1 cut(s) 560
HpySE526I ACGT 2 cut(s) 316, 408
Hsp92II CATG 5 cut(s) 128, 160, 585, 626, 763
HspAI GCGC 1 cut(s) 711
Kzo9I GATC 4 cut(s) 192, 424, 553, 639
LmnI GCTCC 1 cut(s) 245
Lsp1109I GCAGC 2 cut(s) 788, 796
LweI GCATC 3 cut(s) 646, 742, 758
MaeI CTAG 4 cut(s) 255, 662, 701, 808
MaeII ACGT 2 cut(s) 316, 408
MaeIII GTNAC 1 cut(s) 84
MalI GATC 4 cut(s) 194, 426, 555, 641
MboI GATC 4 cut(s) 192, 424, 553, 639
MboII GAAGA 3 cut(s) 442, 455, 665
MhlI GDGCHC 1 cut(s) 322
MluCI AATT 4 cut(s) 222, 330, 523, 692
MnlI CCTC 6 cut(s) 262, 341, 344, 410, 428, 739
MseI TTAA 4 cut(s) 501, 507, 588, 771
MspCI CTTAAG 1 cut(s) 587
MspI CCGG 3 cut(s) 104, 219, 607
MspR9I CCNGG 2 cut(s) 104, 608
Mva1269I GAATGC 1 cut(s) 608
MwoI GCNNNNNNNGC 4 cut(s) 11, 317, 326, 746
NciI CCSGG 2 cut(s) 104, 608
NcoI CCATGG 1 cut(s) 622
NdeI CATATG 1 cut(s) 392
NdeII GATC 4 cut(s) 192, 424, 553, 639
NheI GCTAGC 1 cut(s) 254
NlaIII CATG 5 cut(s) 128, 160, 585, 626, 763
NlaIV GGNNCC 1 cut(s) 241
NmeAIII GCCGAG 1 cut(s) 766
PctI GAATGC 1 cut(s) 608
PfeI GAWTC 2 cut(s) 42, 578
PkrI GCNGC 3 cut(s) 13, 778, 786
Psp1406I AACGTT 1 cut(s) 408
PspN4I GGNNCC 1 cut(s) 241
PspPI GGNCC 1 cut(s) 106
PstI CTGCAG 1 cut(s) 786
PstNI CAGNNNCTG 1 cut(s) 11
RsaI GTAC 4 cut(s) 51, 397, 565, 725
RsaNI GTAC 4 cut(s) 50, 396, 564, 724
SaqAI TTAA 4 cut(s) 501, 507, 588, 771
SatI GCNGC 3 cut(s) 12, 777, 785
Sau3AI GATC 4 cut(s) 192, 424, 553, 639
Sau96I GGNCC 1 cut(s) 106
ScaI AGTACT 1 cut(s) 565
ScrFI CCNGG 2 cut(s) 104, 608
SduI GDGCHC 1 cut(s) 322
SfaNI GCATC 3 cut(s) 646, 742, 758
SfcI CTRYAG 1 cut(s) 782
SinI GGWCC 1 cut(s) 106
SmlI CTYRAG 1 cut(s) 587
SmoI CTYRAG 1 cut(s) 587
Sse9I AATT 4 cut(s) 222, 330, 523, 692
SspMI CTAG 4 cut(s) 255, 662, 701, 808
StyD4I CCNGG 2 cut(s) 102, 606
StyI CCWWGG 3 cut(s) 141, 537, 622
TaaI ACNGT 3 cut(s) 65, 344, 400
TaiI ACGT 2 cut(s) 319, 411
TaqI TCGA 1 cut(s) 133
TaqII GACCGA 1 cut(s) 742
TasI AATT 4 cut(s) 222, 330, 523, 692
TatI WGTACW 2 cut(s) 563, 723
TfiI GAWTC 2 cut(s) 42, 578
Tru1I TTAA 4 cut(s) 501, 507, 588, 771
Tru9I TTAA 4 cut(s) 501, 507, 588, 771
TscAI CASTG 3 cut(s) 30, 183, 331
TseI GCWGC 3 cut(s) 11, 776, 784
TspDTI ATGAA 3 cut(s) 201, 481, 757
TspGWI ACGGA 1 cut(s) 194
TspRI CASTG 3 cut(s) 30, 183, 331
Vha464I CTTAAG 1 cut(s) 587
VneI GTGCAC 1 cut(s) 318
VpaK11BI GGWCC 1 cut(s) 106
XspI CTAG 4 cut(s) 255, 662, 701, 808
ZrmI AGTACT 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.