Rh2DG082500

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000515
Physical Location & Seq
Reverse (-)
6472376 .. 6475064
2689 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG082500.1

Sequence Viewer

Length: 582 bp
ATGGATAGCGTAAGTACTGATATGCATGAATCATGCCTTAAGATTGACAAAGAATGCCGCTGTGAAGATCGAGAAGCAAAGACTGTGCAAAACGAATCTTGGAATAACATGGCAGAGGCTGCACAGATGCTAGGAGGCGGTGATGTGCTCCTGTATGTTTCAAAGTATCATGACATTCCTACGGTTGTCGAAGCTTCTGCACGCTATGACCTAAAGAAAGGCATTGAAGGACGCCTGGGGAAAGACTTTATGGAGAAGATCAAAAAGGAAGGATTCGTTGATATTGGAAGTCCTAAATTTCGGGTGACTGAGGAAAGCTTGATGGACCGCCTAAGCACCGATATGTGTGAATCGTGCCTCAAGATTGACAAAGATTGCCGGGTGCTAATAGTCCATGGATCTGCTGATGAGATCACTCCTGTTGAAGATGCATTTGAGTTTGCCAAGATCATACCTAACCACAAAATACATATTATAGAAGGAGCTGATCATTGTTACAGATCGCATCAGGCCGAGTTAGACACAGTTGTTGTGGACTTCATAAAAGCAGCTCTGCAGCAGGACAAGGCTTCTTCCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

193

Amino Acids

21.7

Weight (kDa)

5.02

Isoelectric Point (pI)

39.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47590 AT3G47590
fragaria_vesca FvH4_1g07650 FvH4_1g07670 FvH4_1g07671 FvH4_1g07680 FvH4_1g07690 FvH4_1g07700 FvH4_1g07700
malus_domestica MD02G1080900.v1.1 MD02G1081000.v1.1 MD02G1081300.v1.1 MD02G1081400.v1.1 MD02G1094000.v1.1 MD15G1208700.v1.1
prunus_persica Prupe.7G208200_v2.0.a1 Prupe.7G208200_v2.0.a1
pyrus_communis pycom02g07440 pycom15g18480
rosa_chinensis RchiOBHm_Chr2g0093511 RchiOBHm_Chr2g0093521 RchiOBHm_Chr2g0093531 RchiOBHm_Chr2g0093541 RchiOBHm_Chr2g0093551 RchiOBHm_Chr2g0093561
rosa_laevigata RLG00000016413 RLG00000016414 RLG00000016415 RLG00000016416 RLG00000016417
rosa_multiflora Rmu_sc0004423.1_g000003 Rmu_sc0004423.1_g000004 Rmu_sc0004423.1_g000005 Rmu_sc0004423.1_g000006 Rmu_sc0006964.1_g000003 Rmu_sc0039672.1_g000001 Rmu_sc0039672.1_g000003
rosa_roxburghii Rroxscaffold_2G00147990 Rroxscaffold_2G00148000 Rroxscaffold_2G00148010 Rroxscaffold_2G00148020 Rroxscaffold_4G00313540
rosa_rugosa Rorug01G0140600.1 Rorug01G0140700.1 Rorug02G0037000 Rorug02G0037100 Rorug02G0037200 Rorug02G0037300 Rorug02G0037400 Rorug02G0037500 Rorug02G0037600
rosa_samantha Rh2AG083400 Rh2AG083500 Rh2AG083700 Rh2AG083800 Rh2AG083900 Rh2AG084000 Rh2BG084200 Rh2BG084300 Rh2BG084400 Rh2BG084500 Rh2BG084600 Rh2CG086300 Rh2CG086400 Rh2CG086500 Rh2CG086700 Rh2CG086800 Rh2CG086900 Rh2DG082200 Rh2DG082300 Rh2DG082400 Rh2DG082500 Rh2DG082600 Rh2DG082700
rosa_wichuraiana Rw1G013200 Rw2G006360 Rw2G006370 Rw2G006380 Rw2G006390 Rw2G006400 Rw2G007130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 58, 138, 328
AclWI GGATC 1 cut(s) 406
AcsI RAATTY 1 cut(s) 296
AcyI GRCGYC 1 cut(s) 232
AfaI GTAC 1 cut(s) 16
AflII CTTAAG 1 cut(s) 38
AgsI TTSAA 3 cut(s) 162, 227, 425
AjnI CCWGG 1 cut(s) 234
AluBI AGCT 4 cut(s) 194, 318, 485, 551
AluI AGCT 4 cut(s) 194, 318, 485, 551
Alw21I GWGCWC 1 cut(s) 150
AlwI GGATC 1 cut(s) 406
AlwNI CAGNNNCTG 1 cut(s) 119
AoxI GGCC 1 cut(s) 510
ApeKI GCWGC 3 cut(s) 119, 548, 556
ApoI RAATTY 1 cut(s) 296
AspS9I GGNCC 1 cut(s) 325
AsuC2I CCSGG 1 cut(s) 380
AsuHPI GGTGA 2 cut(s) 152, 316
AvaII GGWCC 1 cut(s) 325
Bbv12I GWGCWC 1 cut(s) 150
BbvI GCAGC 3 cut(s) 106, 560, 568
BccI CCATC 1 cut(s) 316
BcgI CGANNNNNNTGC 2 cut(s) 179, 213
BciT130I CCWGG 1 cut(s) 236
BclI TGATCA 1 cut(s) 487
BcnI CCSGG 1 cut(s) 380
BfaI CTAG 2 cut(s) 131, 580
BfmI CTRYAG 1 cut(s) 554
BfrI CTTAAG 1 cut(s) 38
BisI GCNGC 4 cut(s) 58, 120, 549, 557
BlsI GCNGC 4 cut(s) 59, 121, 550, 558
BmcAI AGTACT 1 cut(s) 16
Bme1390I CCNGG 2 cut(s) 236, 380
Bme18I GGWCC 1 cut(s) 325
BmgT120I GGNCC 1 cut(s) 325
BmrFI CCNGG 2 cut(s) 236, 380
BmsI GCATC 3 cut(s) 117, 418, 514
Bpu10I CCTNAGC 1 cut(s) 332
BpuEI CTTGAG 1 cut(s) 344
BpuMI CCSGG 1 cut(s) 380
BsaHI GRCGYC 1 cut(s) 232
BsaJI CCNNGG 2 cut(s) 235, 394
BseBI CCWGG 1 cut(s) 236
BseDI CCNNGG 2 cut(s) 235, 394
BseMII CTCAG 1 cut(s) 300
BseXI GCAGC 3 cut(s) 106, 560, 568
BsgI GTGCAG 2 cut(s) 105, 183
BshFI GGCC 1 cut(s) 512
BsiHKAI GWGCWC 1 cut(s) 150
BsiSI CCGG 1 cut(s) 379
BsmI GAATGC 1 cut(s) 59
BsnI GGCC 1 cut(s) 512
Bsp1286I GDGCHC 1 cut(s) 150
Bsp143I GATC 7 cut(s) 67, 258, 398, 411, 447, 487, 500
Bsp19I CCATGG 1 cut(s) 394
BspACI CCGC 3 cut(s) 58, 138, 328
BspANI GGCC 1 cut(s) 512
BspCNI CTCAG 1 cut(s) 301
BspHI TCATGA 1 cut(s) 169
BspMAI CTGCAG 1 cut(s) 558
BspPI GGATC 1 cut(s) 406
BspTI CTTAAG 1 cut(s) 38
BssECI CCNNGG 2 cut(s) 235, 394
BssMI GATC 7 cut(s) 67, 258, 398, 411, 447, 487, 500
BssNI GRCGYC 1 cut(s) 232
BssT1I CCWWGG 1 cut(s) 394
Bst2UI CCWGG 1 cut(s) 236
Bst4CI ACNGT 3 cut(s) 85, 184, 526
BstACI GRCGYC 1 cut(s) 232
BstAFI CTTAAG 1 cut(s) 38
BstAPI GCANNNNNTGC 1 cut(s) 119
BstC8I GCNNGC 1 cut(s) 202
BstDEI CTNAG 2 cut(s) 309, 332
BstDSI CCRYGG 1 cut(s) 394
BstKTI GATC 7 cut(s) 70, 261, 401, 414, 450, 490, 503
BstMBI GATC 7 cut(s) 67, 258, 398, 411, 447, 487, 500
BstMWI GCNNNNNNNGC 1 cut(s) 119
BstNI CCWGG 1 cut(s) 236
BstSCI CCNGG 2 cut(s) 234, 378
BstSFI CTRYAG 1 cut(s) 554
BstV1I GCAGC 3 cut(s) 106, 560, 568
BstX2I RGATCY 1 cut(s) 398
BstYI RGATCY 1 cut(s) 398
BsuRI GGCC 1 cut(s) 512
BtgI CCRYGG 1 cut(s) 394
Cac8I GCNNGC 1 cut(s) 202
CaiI CAGNNNCTG 1 cut(s) 119
CciI TCATGA 1 cut(s) 169
Cfr13I GGNCC 1 cut(s) 325
CseI GACGC 1 cut(s) 240
Csp6I GTAC 1 cut(s) 15
CviAII CATG 5 cut(s) 26, 33, 109, 170, 395
CviJI RGCY 7 cut(s) 119, 194, 318, 485, 512, 551, 569
CviKI_1 RGCY 7 cut(s) 119, 194, 318, 485, 512, 551, 569
CviQI GTAC 1 cut(s) 15
DdeI CTNAG 2 cut(s) 309, 332
DpnI GATC 7 cut(s) 69, 260, 400, 413, 449, 489, 502
DpnII GATC 7 cut(s) 67, 258, 398, 411, 447, 487, 500
Eco130I CCWWGG 1 cut(s) 394
Eco47I GGWCC 1 cut(s) 325
EcoRII CCWGG 1 cut(s) 234
EcoT14I CCWWGG 1 cut(s) 394
EcoT22I ATGCAT 2 cut(s) 27, 433
ErhI CCWWGG 1 cut(s) 394
FaeI CATG 5 cut(s) 29, 36, 112, 173, 398
FatI CATG 5 cut(s) 25, 32, 108, 169, 394
FbaI TGATCA 1 cut(s) 487
Fnu4HI GCNGC 4 cut(s) 58, 120, 549, 557
Fsp4HI GCNGC 4 cut(s) 58, 120, 549, 557
FspBI CTAG 2 cut(s) 131, 580
GluI GCNGC 4 cut(s) 58, 120, 549, 557
HaeIII GGCC 1 cut(s) 512
HapII CCGG 1 cut(s) 379
HgaI GACGC 1 cut(s) 240
Hin1I GRCGYC 1 cut(s) 232
Hin1II CATG 5 cut(s) 29, 36, 112, 173, 398
HindIII AAGCTT 2 cut(s) 192, 316
HinfI GANTC 4 cut(s) 29, 95, 273, 350
HpaII CCGG 1 cut(s) 379
HphI GGTGA 2 cut(s) 152, 316
Hpy166II GTNNAC 1 cut(s) 535
Hpy188III TCNNGA 3 cut(s) 71, 170, 361
Hpy8I GTNNAC 1 cut(s) 535
HpyAV CCTTC 3 cut(s) 221, 263, 473
HpyCH4III ACNGT 3 cut(s) 85, 184, 526
HpyCH4V TGCA 6 cut(s) 25, 88, 122, 200, 431, 556
HpyF10VI GCNNNNNNNGC 1 cut(s) 119
HpyF3I CTNAG 2 cut(s) 309, 332
Hsp92I GRCGYC 1 cut(s) 232
Hsp92II CATG 5 cut(s) 29, 36, 112, 173, 398
Ksp22I TGATCA 1 cut(s) 487
Kzo9I GATC 7 cut(s) 67, 258, 398, 411, 447, 487, 500
LmnI GCTCC 2 cut(s) 153, 482
LpnPI CCDG 7 cut(s) 164, 221, 248, 392, 432, 494, 545
Lsp1109I GCAGC 3 cut(s) 106, 560, 568
LweI GCATC 3 cut(s) 117, 418, 514
MaeI CTAG 2 cut(s) 131, 580
MaeIII GTNAC 2 cut(s) 304, 494
MalI GATC 7 cut(s) 69, 260, 400, 413, 449, 489, 502
MboI GATC 7 cut(s) 67, 258, 398, 411, 447, 487, 500
MboII GAAGA 4 cut(s) 77, 268, 437, 564
MflI RGATCY 1 cut(s) 398
MhlI GDGCHC 1 cut(s) 150
MluCI AATT 1 cut(s) 296
MnlI CCTC 4 cut(s) 109, 128, 304, 368
Mph1103I ATGCAT 2 cut(s) 27, 433
MseI TTAA 1 cut(s) 39
MslI CAYNNNNRTG 1 cut(s) 341
MspA1I CMGCKG 1 cut(s) 60
MspCI CTTAAG 1 cut(s) 38
MspI CCGG 1 cut(s) 379
MspR9I CCNGG 2 cut(s) 236, 380
Mva1269I GAATGC 1 cut(s) 59
MvaI CCWGG 1 cut(s) 236
MwoI GCNNNNNNNGC 1 cut(s) 119
NciI CCSGG 1 cut(s) 380
NcoI CCATGG 1 cut(s) 394
NdeII GATC 7 cut(s) 67, 258, 398, 411, 447, 487, 500
NlaIII CATG 5 cut(s) 29, 36, 112, 173, 398
NmeAIII GCCGAG 1 cut(s) 538
NmuCI GTSAC 1 cut(s) 304
NsiI ATGCAT 2 cut(s) 27, 433
PagI TCATGA 1 cut(s) 169
PctI GAATGC 1 cut(s) 59
PfeI GAWTC 4 cut(s) 29, 95, 273, 350
PkrI GCNGC 4 cut(s) 59, 121, 550, 558
Psp6I CCWGG 1 cut(s) 234
PspGI CCWGG 1 cut(s) 234
PspPI GGNCC 1 cut(s) 325
PstI CTGCAG 1 cut(s) 558
PstNI CAGNNNCTG 1 cut(s) 119
PsuI RGATCY 1 cut(s) 398
RsaI GTAC 1 cut(s) 16
RsaNI GTAC 1 cut(s) 15
RseI CAYNNNNRTG 1 cut(s) 341
SaqAI TTAA 1 cut(s) 39
SatI GCNGC 4 cut(s) 58, 120, 549, 557
Sau3AI GATC 7 cut(s) 67, 258, 398, 411, 447, 487, 500
Sau96I GGNCC 1 cut(s) 325
ScaI AGTACT 1 cut(s) 16
ScrFI CCNGG 2 cut(s) 236, 380
SduI GDGCHC 1 cut(s) 150
SetI ASST 6 cut(s) 196, 213, 320, 457, 487, 553
SfaNI GCATC 3 cut(s) 117, 418, 514
SfcI CTRYAG 1 cut(s) 554
SinI GGWCC 1 cut(s) 325
SmiMI CAYNNNNRTG 1 cut(s) 341
SmlI CTYRAG 2 cut(s) 38, 359
SmoI CTYRAG 2 cut(s) 38, 359
Sse9I AATT 1 cut(s) 296
SsiI CCGC 3 cut(s) 58, 138, 328
SspMI CTAG 2 cut(s) 131, 580
StyD4I CCNGG 2 cut(s) 234, 378
StyI CCWWGG 1 cut(s) 394
TaaI ACNGT 3 cut(s) 85, 184, 526
TaqI TCGA 2 cut(s) 70, 189
TasI AATT 1 cut(s) 296
TatI WGTACW 1 cut(s) 14
TauI GCSGC 1 cut(s) 60
TfiI GAWTC 4 cut(s) 29, 95, 273, 350
Tru1I TTAA 1 cut(s) 39
Tru9I TTAA 1 cut(s) 39
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 3 cut(s) 119, 548, 556
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 2 cut(s) 42, 529
Vha464I CTTAAG 1 cut(s) 38
VpaK11BI GGWCC 1 cut(s) 325
XapI RAATTY 1 cut(s) 296
XspI CTAG 2 cut(s) 131, 580
ZrmI AGTACT 1 cut(s) 16
Zsp2I ATGCAT 2 cut(s) 27, 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.