MD02G1080900.v1.1

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000515
Physical Location & Seq
Reverse (-)
6367014 .. 6369716
2703 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1080900.v1.1.491

Sequence Viewer

Length: 975 bp
ATGTGTGATGTGTCTTCCTTTCTGTACTCAAGTTCTAATCCAAGGTTTATTTATACTTTAATGAGGGGTTTTTGGCTAAATTGCAAAGGTTTATTAAGCCAAGATTGCATCTCAGAGCAACAAGAGCAGCAAGCAAAAGTTTCTAGCCCAAAACGAATCTTGAAAATGGCAGAAGCTGCAAAAAACCCAGTGGGTCAGCAACAGAGAATCATCGTACCCAACAAACACGGTGAAAAGCTTGTGGGATTGTTACACGAAACCGGTTCCGCGGACCTTGTTATCTTATGTCATGGTTTTCGAGACACCAAGGAACACTATATTATGGTGAACCTTGCTGTTGCACTGGAAAATGAAGGGATCAGTTCCTTCCGTTTTGACTTTGCCGGAAATGGGGAAAGTGAAGGCACCTTTCAGTTTGGTAACTATCGGAGAGAGGCGGATGACTTGCGCTCTGTGGTCGAGTACTTCTCTGGGGCAAAACGTGCACCCAGTACAATTGTTGGACACAGTAAAGGAGGCGATGCTGTGCTCCTGTATGCTTCTACGTATCATGACATTCGTACGGTTGTCAATATTTCTGGACGTTATGAACTGAAGAAAGGCATCGAAGAACGCTTGGGGAAAGACTTTATGGAAGTAATCAAGAAGGAAGGGTTCATTGATGTTAAGAATAAGTCAGGAGATGGTAGTTATCGTGTGACCGAGGAGAGTTTGATGGATCGCCTAAGTACTGATATGCACCAATCATGCCTTCAGATTGACAAAGAATGCCGGGTGGTGACAATCCATGGAACTGCCGACGAGGTCATCCCTGTTGAAGATGCATTAGAGTTTGCCAAGATAATACATAACCACAAATTACATCTTATAGAAGGCGCTAATCATTCATACACCTCGCATCAAGCCGAGTTAGCATCGCTCGTCGTGGACTTCATTAAGGCAGCTCCGCAGCAGGACAAGGCTACTTCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

325

Amino Acids

36.19

Weight (kDa)

6.12

Isoelectric Point (pI)

43.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 92 - 202 1.8e-12 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 92 - 194 4.6e-06 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 93 - 305 4.2e-06 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47590 AT3G47590
fragaria_vesca FvH4_1g07650 FvH4_1g07670 FvH4_1g07671 FvH4_1g07680 FvH4_1g07690 FvH4_1g07700 FvH4_1g07700
malus_domestica MD02G1080900.v1.1 MD02G1081000.v1.1 MD02G1081300.v1.1 MD02G1081400.v1.1 MD02G1094000.v1.1 MD15G1208700.v1.1
prunus_persica Prupe.7G208200_v2.0.a1 Prupe.7G208200_v2.0.a1
pyrus_communis pycom02g07440 pycom15g18480
rosa_chinensis RchiOBHm_Chr2g0093511 RchiOBHm_Chr2g0093521 RchiOBHm_Chr2g0093531 RchiOBHm_Chr2g0093541 RchiOBHm_Chr2g0093551 RchiOBHm_Chr2g0093561
rosa_laevigata RLG00000016413 RLG00000016414 RLG00000016415 RLG00000016416 RLG00000016417
rosa_multiflora Rmu_sc0004423.1_g000003 Rmu_sc0004423.1_g000004 Rmu_sc0004423.1_g000005 Rmu_sc0004423.1_g000006 Rmu_sc0006964.1_g000003 Rmu_sc0039672.1_g000001 Rmu_sc0039672.1_g000003
rosa_roxburghii Rroxscaffold_2G00147990 Rroxscaffold_2G00148000 Rroxscaffold_2G00148010 Rroxscaffold_2G00148020 Rroxscaffold_4G00313540
rosa_rugosa Rorug01G0140600.1 Rorug01G0140700.1 Rorug02G0037000 Rorug02G0037100 Rorug02G0037200 Rorug02G0037300 Rorug02G0037400 Rorug02G0037500 Rorug02G0037600
rosa_samantha Rh2AG083400 Rh2AG083500 Rh2AG083700 Rh2AG083800 Rh2AG083900 Rh2AG084000 Rh2BG084200 Rh2BG084300 Rh2BG084400 Rh2BG084500 Rh2BG084600 Rh2CG086300 Rh2CG086400 Rh2CG086500 Rh2CG086700 Rh2CG086800 Rh2CG086900 Rh2DG082200 Rh2DG082300 Rh2DG082400 Rh2DG082500 Rh2DG082600 Rh2DG082700
rosa_wichuraiana Rw1G013200 Rw2G006360 Rw2G006370 Rw2G006380 Rw2G006390 Rw2G006400 Rw2G007130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 404
AccII CGCG 1 cut(s) 269
AciI CCGC 4 cut(s) 267, 269, 437, 947
AclWI GGATC 2 cut(s) 365, 726
AcuI CTGAAG 2 cut(s) 614, 737
AfaI GTAC 6 cut(s) 26, 216, 464, 493, 562, 730
AfiI CCNNNNNNNGG 1 cut(s) 390
AgeI ACCGGT 1 cut(s) 260
AgsI TTSAA 3 cut(s) 163, 818, 969
AjuI GAANNNNNNNTTGG 2 cut(s) 735, 767
AluBI AGCT 3 cut(s) 176, 238, 944
AluI AGCT 3 cut(s) 176, 238, 944
Alw21I GWGCWC 2 cut(s) 487, 531
Alw26I GTCTC 1 cut(s) 294
Alw44I GTGCAC 1 cut(s) 483
AlwI GGATC 2 cut(s) 365, 726
AlwNI CAGNNNCTG 1 cut(s) 176
ApaLI GTGCAC 1 cut(s) 483
ApeKI GCWGC 4 cut(s) 127, 176, 941, 949
AsiGI ACCGGT 1 cut(s) 260
AspLEI GCGC 2 cut(s) 450, 878
AspS9I GGNCC 1 cut(s) 271
AsuC2I CCSGG 1 cut(s) 773
AsuHPI GGTGA 3 cut(s) 242, 337, 790
AvaII GGWCC 1 cut(s) 271
BaeGI GKGCMC 1 cut(s) 487
BanI GGYRCC 1 cut(s) 404
BbsI GAAGAC 1 cut(s) 6
Bbv12I GWGCWC 2 cut(s) 487, 531
BbvI GCAGC 4 cut(s) 139, 163, 953, 961
BccI CCATC 2 cut(s) 677, 709
BcnI CCSGG 1 cut(s) 773
BcoDI GTCTC 1 cut(s) 294
BfaI CTAG 2 cut(s) 144, 973
BfoI RGCGCY 1 cut(s) 879
BisI GCNGC 4 cut(s) 128, 177, 942, 950
BlsI GCNGC 4 cut(s) 129, 178, 943, 951
BmcAI AGTACT 2 cut(s) 464, 730
Bme1390I CCNGG 1 cut(s) 773
Bme18I GGWCC 1 cut(s) 271
BmgT120I GGNCC 1 cut(s) 271
BmiI GGNNCC 2 cut(s) 265, 406
BmrFI CCNGG 1 cut(s) 773
BmrI ACTGGG 2 cut(s) 182, 483
BmsI GCATC 6 cut(s) 117, 511, 612, 811, 907, 923
BmuI ACTGGG 2 cut(s) 182, 483
BpiI GAAGAC 1 cut(s) 6
BplI GAGNNNNNCTC 2 cut(s) 452, 484
BpuEI CTTGAG 1 cut(s) 13
BpuMI CCSGG 1 cut(s) 773
BsaAI YACGTR 1 cut(s) 546
BsaJI CCNNGG 5 cut(s) 41, 267, 306, 702, 787
BsaWI WCCGGW 1 cut(s) 260
Bsc4I CCNNNNNNNGG 1 cut(s) 390
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 3 cut(s) 188, 348, 489
BseDI CCNNGG 5 cut(s) 41, 267, 306, 702, 787
BseGI GGATG 2 cut(s) 445, 807
BseLI CCNNNNNNNGG 1 cut(s) 390
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 3 cut(s) 188, 348, 489
BseRI GAGGAG 1 cut(s) 719
BseSI GKGCMC 1 cut(s) 487
BseXI GCAGC 4 cut(s) 139, 163, 953, 961
Bsh1236I CGCG 1 cut(s) 269
BshNI GGYRCC 1 cut(s) 404
BshTI ACCGGT 1 cut(s) 260
BsiHKAI GWGCWC 2 cut(s) 487, 531
BsiSI CCGG 3 cut(s) 261, 384, 772
BsiWI CGTACG 1 cut(s) 560
BslI CCNNNNNNNGG 1 cut(s) 390
BsmAI GTCTC 1 cut(s) 294
BsmI GAATGC 1 cut(s) 773
Bsp1286I GDGCHC 2 cut(s) 487, 531
Bsp143I GATC 2 cut(s) 357, 718
Bsp19I CCATGG 1 cut(s) 787
BspACI CCGC 4 cut(s) 267, 269, 437, 947
BspCNI CTCAG 1 cut(s) 125
BspFNI CGCG 1 cut(s) 269
BspHI TCATGA 1 cut(s) 550
BspLI GGNNCC 2 cut(s) 265, 406
BspPI GGATC 2 cut(s) 365, 726
BspT107I GGYRCC 1 cut(s) 404
BsrFI RCCGGY 1 cut(s) 260
BsrI ACTGG 3 cut(s) 188, 348, 489
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 5 cut(s) 41, 267, 306, 702, 787
BssMI GATC 2 cut(s) 357, 718
BssT1I CCWWGG 3 cut(s) 41, 306, 787
Bst4CI ACNGT 3 cut(s) 230, 509, 565
BstAPI GCANNNNNTGC 2 cut(s) 176, 482
BstBAI YACGTR 1 cut(s) 546
BstC8I GCNNGC 1 cut(s) 132
BstDEI CTNAG 2 cut(s) 112, 725
BstDSI CCRYGG 2 cut(s) 267, 787
BstF5I GGATG 2 cut(s) 445, 807
BstFNI CGCG 1 cut(s) 269
BstH2I RGCGCY 1 cut(s) 879
BstHHI GCGC 2 cut(s) 450, 878
BstKTI GATC 2 cut(s) 360, 721
BstMAI GTCTC 1 cut(s) 294
BstMBI GATC 2 cut(s) 357, 718
BstMWI GCNNNNNNNGC 5 cut(s) 105, 124, 176, 482, 911
BstSCI CCNGG 1 cut(s) 771
BstSLI GKGCMC 1 cut(s) 487
BstSNI TACGTA 1 cut(s) 546
BstUI CGCG 1 cut(s) 269
BstV1I GCAGC 4 cut(s) 139, 163, 953, 961
BstV2I GAAGAC 1 cut(s) 6
BtgI CCRYGG 2 cut(s) 267, 787
BtgZI GCGATG 2 cut(s) 534, 900
BtsCI GGATG 2 cut(s) 445, 807
BtsIMutI CAGTG 2 cut(s) 195, 341
Cac8I GCNNGC 1 cut(s) 132
CaiI CAGNNNCTG 1 cut(s) 176
CciI TCATGA 1 cut(s) 550
CfoI GCGC 2 cut(s) 450, 878
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 1 cut(s) 271
Cfr42I CCGCGG 1 cut(s) 270
Csp6I GTAC 6 cut(s) 25, 215, 463, 492, 561, 729
CspAI ACCGGT 1 cut(s) 260
CviAII CATG 4 cut(s) 290, 551, 747, 788
CviJI RGCY 8 cut(s) 76, 99, 147, 176, 238, 905, 944, 962
CviKI_1 RGCY 8 cut(s) 76, 99, 147, 176, 238, 905, 944, 962
CviQI GTAC 6 cut(s) 25, 215, 463, 492, 561, 729
DdeI CTNAG 2 cut(s) 112, 725
DpnI GATC 2 cut(s) 359, 720
DpnII GATC 2 cut(s) 357, 718
EciI GGCGGA 1 cut(s) 452
Eco105I TACGTA 1 cut(s) 546
Eco130I CCWWGG 3 cut(s) 41, 306, 787
Eco47I GGWCC 1 cut(s) 271
Eco57I CTGAAG 2 cut(s) 614, 737
EcoT14I CCWWGG 3 cut(s) 41, 306, 787
EcoT22I ATGCAT 1 cut(s) 826
ErhI CCWWGG 3 cut(s) 41, 306, 787
FaeI CATG 4 cut(s) 293, 554, 750, 791
FatI CATG 4 cut(s) 289, 550, 746, 787
Fnu4HI GCNGC 4 cut(s) 128, 177, 942, 950
FokI GGATG 2 cut(s) 452, 794
Fsp4HI GCNGC 4 cut(s) 128, 177, 942, 950
FspBI CTAG 2 cut(s) 144, 973
GlaI GCGC 2 cut(s) 449, 877
GluI GCNGC 4 cut(s) 128, 177, 942, 950
HaeII RGCGCY 1 cut(s) 879
HapII CCGG 3 cut(s) 261, 384, 772
HhaI GCGC 2 cut(s) 450, 878
Hin1II CATG 4 cut(s) 293, 554, 750, 791
Hin6I GCGC 2 cut(s) 448, 876
HinP1I GCGC 2 cut(s) 448, 876
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 2 cut(s) 156, 207
HpaII CCGG 3 cut(s) 261, 384, 772
HphI GGTGA 3 cut(s) 242, 337, 790
Hpy166II GTNNAC 3 cut(s) 328, 485, 928
Hpy188I TCNGA 3 cut(s) 115, 429, 756
Hpy188III TCNNGA 6 cut(s) 160, 299, 551, 579, 643, 678
Hpy8I GTNNAC 3 cut(s) 328, 485, 928
Hpy99I CGWCG 2 cut(s) 803, 926
HpyAV CCTTC 7 cut(s) 347, 376, 395, 640, 644, 761, 866
HpyCH4III ACNGT 3 cut(s) 230, 509, 565
HpyCH4IV ACGT 3 cut(s) 481, 545, 583
HpyCH4V TGCA 7 cut(s) 84, 108, 179, 341, 485, 739, 824
HpyF10VI GCNNNNNNNGC 5 cut(s) 105, 124, 176, 482, 911
HpyF3I CTNAG 2 cut(s) 112, 725
HpySE526I ACGT 3 cut(s) 481, 545, 583
Hsp92II CATG 4 cut(s) 293, 554, 750, 791
HspAI GCGC 2 cut(s) 448, 876
KspI CCGCGG 1 cut(s) 270
Kzo9I GATC 2 cut(s) 357, 718
LmnI GCTCC 2 cut(s) 534, 949
Lsp1109I GCAGC 4 cut(s) 139, 163, 953, 961
LweI GCATC 6 cut(s) 117, 511, 612, 811, 907, 923
MaeI CTAG 2 cut(s) 144, 973
MaeII ACGT 3 cut(s) 481, 545, 583
MaeIII GTNAC 4 cut(s) 249, 419, 697, 778
MalI GATC 2 cut(s) 359, 720
MboI GATC 2 cut(s) 357, 718
MboII GAAGA 4 cut(s) 6, 607, 620, 830
MfeI CAATTG 1 cut(s) 495
MhlI GDGCHC 2 cut(s) 487, 531
MluCI AATT 3 cut(s) 79, 495, 857
MmeI TCCRAC 1 cut(s) 481
MnlI CCTC 6 cut(s) 57, 427, 509, 697, 796, 904
Mph1103I ATGCAT 1 cut(s) 826
MseI TTAA 4 cut(s) 59, 95, 666, 936
MspA1I CMGCKG 1 cut(s) 269
MspI CCGG 3 cut(s) 261, 384, 772
MspR9I CCNGG 1 cut(s) 773
MunI CAATTG 1 cut(s) 495
Mva1269I GAATGC 1 cut(s) 773
MvnI CGCG 1 cut(s) 269
MwoI GCNNNNNNNGC 5 cut(s) 105, 124, 176, 482, 911
NciI CCSGG 1 cut(s) 773
NcoI CCATGG 1 cut(s) 787
NdeII GATC 2 cut(s) 357, 718
NlaIII CATG 4 cut(s) 293, 554, 750, 791
NlaIV GGNNCC 2 cut(s) 265, 406
NmeAIII GCCGAG 1 cut(s) 931
NmuCI GTSAC 2 cut(s) 697, 778
NsiI ATGCAT 1 cut(s) 826
PagI TCATGA 1 cut(s) 550
PctI GAATGC 1 cut(s) 773
PfeI GAWTC 2 cut(s) 156, 207
Pfl23II CGTACG 1 cut(s) 560
PflFI GACNNNGTC 1 cut(s) 803
PinAI ACCGGT 1 cut(s) 260
PkrI GCNGC 4 cut(s) 129, 178, 943, 951
Ppu21I YACGTR 1 cut(s) 546
PspLI CGTACG 1 cut(s) 560
PspN4I GGNNCC 2 cut(s) 265, 406
PspPI GGNCC 1 cut(s) 271
PstNI CAGNNNCTG 1 cut(s) 176
PsyI GACNNNGTC 1 cut(s) 803
RsaI GTAC 6 cut(s) 26, 216, 464, 493, 562, 730
RsaNI GTAC 6 cut(s) 25, 215, 463, 492, 561, 729
SacII CCGCGG 1 cut(s) 270
SaqAI TTAA 4 cut(s) 59, 95, 666, 936
SatI GCNGC 4 cut(s) 128, 177, 942, 950
Sau3AI GATC 2 cut(s) 357, 718
Sau96I GGNCC 1 cut(s) 271
ScaI AGTACT 2 cut(s) 464, 730
ScrFI CCNGG 1 cut(s) 773
SduI GDGCHC 2 cut(s) 487, 531
SfaNI GCATC 6 cut(s) 117, 511, 612, 811, 907, 923
Sfr303I CCGCGG 1 cut(s) 270
SgrBI CCGCGG 1 cut(s) 270
SinI GGWCC 1 cut(s) 271
SmlI CTYRAG 1 cut(s) 28
SmoI CTYRAG 1 cut(s) 28
SnaBI TACGTA 1 cut(s) 546
Sse9I AATT 3 cut(s) 79, 495, 857
SsiI CCGC 4 cut(s) 267, 269, 437, 947
SspI AATATT 1 cut(s) 574
SspMI CTAG 2 cut(s) 144, 973
StyD4I CCNGG 1 cut(s) 771
StyI CCWWGG 3 cut(s) 41, 306, 787
TaaI ACNGT 3 cut(s) 230, 509, 565
TaiI ACGT 3 cut(s) 484, 548, 586
TaqI TCGA 3 cut(s) 298, 459, 606
TaqII GACCGA 1 cut(s) 716
TasI AATT 3 cut(s) 79, 495, 857
TatI WGTACW 4 cut(s) 24, 462, 491, 728
TfiI GAWTC 2 cut(s) 156, 207
Tru1I TTAA 4 cut(s) 59, 95, 666, 936
Tru9I TTAA 4 cut(s) 59, 95, 666, 936
TscAI CASTG 2 cut(s) 195, 348
TseFI GTSAC 2 cut(s) 697, 778
TseI GCWGC 4 cut(s) 127, 176, 941, 949
Tsp45I GTSAC 2 cut(s) 697, 778
TspDTI ATGAA 5 cut(s) 366, 603, 646, 876, 922
TspGWI ACGGA 1 cut(s) 359
TspRI CASTG 2 cut(s) 195, 348
Tth111I GACNNNGTC 1 cut(s) 803
VneI GTGCAC 1 cut(s) 483
VpaK11BI GGWCC 1 cut(s) 271
XspI CTAG 2 cut(s) 144, 973
ZrmI AGTACT 2 cut(s) 464, 730
Zsp2I ATGCAT 1 cut(s) 826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.