Rorug02G0037400

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000515
Physical Location & Seq
Forward (+)
2930029 .. 2930960
932 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0037400.1

Sequence Viewer

Length: 594 bp
ATGACCTTCCCATTTTTTGGGGGTTGCGGTGTGTTGGGCTCCCATATTGTTTTCGTCAGCAGCCTGAAACAAAACCCTCCTGGGCGCTCTTTGAGTGGATGTGGCTCCAGTCGAGAAGCTTATGTGTTTGATACCAAGCATCTCGAGAAGGGGATCGTGAAAATGGATGGGACTCTAAACCAAGGGAAACACCAACCGTTGACGGTGGAGGTGGCCGGCAAAATCTACTCTCTATGCAATTATGAGTTTTCTTACTATCCTCCTGATTCATTTGAGATGAACCGAGAAACAATAACACTAATCCATGAGGTGCAGCCGTCGGAGCTCCCAGAACTCCCTGGTGAACTAGCAGGCTATCATTTCCTCCATCTAGGAGGTTACAATGTCTGCATTGTCATCCCCAATGATCCACCTAATCAATTTTATAAGCATGGGAGTATGAACATGAGGTGTGATGCCATACCATTTCAATTTGAATTTGACAAGGAAAATTGTTTCACTGTCCAATTCCTGCCTCCTCGCATCTTGGAATACCATACCAACCCATCCTGTTTTCCAAAGCTTTCTCCTAACTTTGGTTTCTTTGTAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

197

Amino Acids

22.23

Weight (kDa)

5.87

Isoelectric Point (pI)

36.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47590 AT3G47590
fragaria_vesca FvH4_1g07650 FvH4_1g07670 FvH4_1g07671 FvH4_1g07680 FvH4_1g07690 FvH4_1g07700 FvH4_1g07700
malus_domestica MD02G1080900.v1.1 MD02G1081000.v1.1 MD02G1081300.v1.1 MD02G1081400.v1.1 MD02G1094000.v1.1 MD15G1208700.v1.1
prunus_persica Prupe.7G208200_v2.0.a1 Prupe.7G208200_v2.0.a1
pyrus_communis pycom02g07440 pycom15g18480
rosa_chinensis RchiOBHm_Chr2g0093511 RchiOBHm_Chr2g0093521 RchiOBHm_Chr2g0093531 RchiOBHm_Chr2g0093541 RchiOBHm_Chr2g0093551 RchiOBHm_Chr2g0093561
rosa_laevigata RLG00000016413 RLG00000016414 RLG00000016415 RLG00000016416 RLG00000016417
rosa_multiflora Rmu_sc0004423.1_g000003 Rmu_sc0004423.1_g000004 Rmu_sc0004423.1_g000005 Rmu_sc0004423.1_g000006 Rmu_sc0006964.1_g000003 Rmu_sc0039672.1_g000001 Rmu_sc0039672.1_g000003
rosa_roxburghii Rroxscaffold_2G00147990 Rroxscaffold_2G00148000 Rroxscaffold_2G00148010 Rroxscaffold_2G00148020 Rroxscaffold_4G00313540
rosa_rugosa Rorug01G0140600.1 Rorug01G0140700.1 Rorug02G0037000 Rorug02G0037100 Rorug02G0037200 Rorug02G0037300 Rorug02G0037400 Rorug02G0037500 Rorug02G0037600
rosa_samantha Rh2AG083400 Rh2AG083500 Rh2AG083700 Rh2AG083800 Rh2AG083900 Rh2AG084000 Rh2BG084200 Rh2BG084300 Rh2BG084400 Rh2BG084500 Rh2BG084600 Rh2CG086300 Rh2CG086400 Rh2CG086500 Rh2CG086700 Rh2CG086800 Rh2CG086900 Rh2DG082200 Rh2DG082300 Rh2DG082400 Rh2DG082500 Rh2DG082600 Rh2DG082700
rosa_wichuraiana Rw1G013200 Rw2G006360 Rw2G006370 Rw2G006380 Rw2G006390 Rw2G006400 Rw2G007130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 426
AccB7I CCANNNNNTGG 1 cut(s) 17
AciI CCGC 1 cut(s) 27
AclWI GGATC 2 cut(s) 161, 401
AcoI YGGCCR 1 cut(s) 213
AcsI RAATTY 1 cut(s) 476
AfiI CCNNNNNNNGG 2 cut(s) 17, 575
AgsI TTSAA 2 cut(s) 470, 476
AjnI CCWGG 2 cut(s) 79, 337
AluBI AGCT 3 cut(s) 119, 325, 562
AluI AGCT 3 cut(s) 119, 325, 562
Alw21I GWGCWC 1 cut(s) 327
AlwI GGATC 2 cut(s) 161, 401
Ama87I CYCGRG 1 cut(s) 143
AoxI GGCC 1 cut(s) 213
ApeKI GCWGC 2 cut(s) 60, 313
ApoI RAATTY 1 cut(s) 476
AspLEI GCGC 1 cut(s) 87
AsuHPI GGTGA 1 cut(s) 353
AvaI CYCGRG 1 cut(s) 143
BanII GRGCYC 2 cut(s) 41, 327
Bbv12I GWGCWC 1 cut(s) 327
BbvI GCAGC 2 cut(s) 72, 325
BccI CCATC 3 cut(s) 161, 375, 553
BceAI ACGGC 1 cut(s) 301
BciT130I CCWGG 2 cut(s) 81, 339
BfaI CTAG 2 cut(s) 347, 371
BfoI RGCGCY 1 cut(s) 88
BisI GCNGC 2 cut(s) 61, 314
BlsI GCNGC 2 cut(s) 62, 315
Bme1390I CCNGG 2 cut(s) 81, 339
BmeT110I CYCGRG 1 cut(s) 143
BmiI GGNNCC 2 cut(s) 40, 106
BmrFI CCNGG 2 cut(s) 81, 339
BmsI GCATC 3 cut(s) 148, 445, 531
BpmI CTGGAG 1 cut(s) 91
BsaJI CCNNGG 3 cut(s) 80, 181, 337
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 575
Bse118I RCCGGY 1 cut(s) 215
Bse1I ACTGG 1 cut(s) 108
BseBI CCWGG 2 cut(s) 81, 339
BseDI CCNNGG 3 cut(s) 80, 181, 337
BseGI GGATG 4 cut(s) 104, 172, 396, 545
BseLI CCNNNNNNNGG 2 cut(s) 17, 575
BseNI ACTGG 1 cut(s) 108
BseRI GAGGAG 1 cut(s) 507
BseXI GCAGC 2 cut(s) 72, 325
BsgI GTGCAG 1 cut(s) 332
BshFI GGCC 1 cut(s) 215
BsiHKAI GWGCWC 1 cut(s) 327
BsiHKCI CYCGRG 1 cut(s) 143
BsiSI CCGG 1 cut(s) 216
BslFI GGGAC 1 cut(s) 184
BslI CCNNNNNNNGG 2 cut(s) 17, 575
BsmFI GGGAC 1 cut(s) 184
BsnI GGCC 1 cut(s) 215
BsoBI CYCGRG 1 cut(s) 143
Bsp1286I GDGCHC 2 cut(s) 41, 327
Bsp143I GATC 2 cut(s) 153, 406
BspACI CCGC 1 cut(s) 27
BspANI GGCC 1 cut(s) 215
BspLI GGNNCC 2 cut(s) 40, 106
BspPI GGATC 2 cut(s) 161, 401
BsrFI RCCGGY 1 cut(s) 215
BsrI ACTGG 1 cut(s) 108
BssAI RCCGGY 1 cut(s) 215
BssECI CCNNGG 3 cut(s) 80, 181, 337
BssMI GATC 2 cut(s) 153, 406
BssT1I CCWWGG 1 cut(s) 181
Bst2UI CCWGG 2 cut(s) 81, 339
Bst4CI ACNGT 3 cut(s) 198, 205, 502
BstC8I GCNNGC 2 cut(s) 217, 352
BstF5I GGATG 4 cut(s) 104, 172, 396, 545
BstH2I RGCGCY 1 cut(s) 88
BstHHI GCGC 1 cut(s) 87
BstKTI GATC 2 cut(s) 156, 409
BstMBI GATC 2 cut(s) 153, 406
BstMWI GCNNNNNNNGC 1 cut(s) 322
BstNI CCWGG 2 cut(s) 81, 339
BstSCI CCNGG 2 cut(s) 79, 337
BstV1I GCAGC 2 cut(s) 72, 325
BsuRI GGCC 1 cut(s) 215
BtsCI GGATG 4 cut(s) 104, 172, 396, 545
BtsIMutI CAGTG 1 cut(s) 498
Cac8I GCNNGC 2 cut(s) 217, 352
CfoI GCGC 1 cut(s) 87
Cfr10I RCCGGY 1 cut(s) 215
CviAII CATG 3 cut(s) 305, 431, 445
CviJI RGCY 9 cut(s) 39, 63, 105, 119, 215, 316, 325, 354, 562
CviKI_1 RGCY 9 cut(s) 39, 63, 105, 119, 215, 316, 325, 354, 562
DpnI GATC 2 cut(s) 155, 408
DpnII GATC 2 cut(s) 153, 406
EaeI YGGCCR 1 cut(s) 213
Ecl136II GAGCTC 1 cut(s) 325
Eco130I CCWWGG 1 cut(s) 181
Eco24I GRGCYC 2 cut(s) 41, 327
Eco53kI GAGCTC 1 cut(s) 325
Eco88I CYCGRG 1 cut(s) 143
EcoICRI GAGCTC 1 cut(s) 325
EcoRII CCWGG 2 cut(s) 79, 337
EcoT14I CCWWGG 1 cut(s) 181
EcoT38I GRGCYC 2 cut(s) 41, 327
ErhI CCWWGG 1 cut(s) 181
FaeI CATG 3 cut(s) 308, 434, 448
FaqI GGGAC 1 cut(s) 184
FatI CATG 3 cut(s) 304, 430, 444
Fnu4HI GCNGC 2 cut(s) 61, 314
FokI GGATG 4 cut(s) 111, 179, 383, 532
FriOI GRGCYC 2 cut(s) 41, 327
Fsp4HI GCNGC 2 cut(s) 61, 314
FspBI CTAG 2 cut(s) 347, 371
GlaI GCGC 1 cut(s) 86
GluI GCNGC 2 cut(s) 61, 314
GsuI CTGGAG 1 cut(s) 91
HaeII RGCGCY 1 cut(s) 88
HaeIII GGCC 1 cut(s) 215
HapII CCGG 1 cut(s) 216
HhaI GCGC 1 cut(s) 87
Hin1II CATG 3 cut(s) 308, 434, 448
Hin6I GCGC 1 cut(s) 85
HinP1I GCGC 1 cut(s) 85
HincII GTYRAC 1 cut(s) 201
HindII GTYRAC 1 cut(s) 201
HindIII AAGCTT 2 cut(s) 117, 560
HinfI GANTC 2 cut(s) 172, 266
HpaII CCGG 1 cut(s) 216
HphI GGTGA 1 cut(s) 353
Hpy166II GTNNAC 2 cut(s) 201, 344
Hpy188I TCNGA 1 cut(s) 322
Hpy188III TCNNGA 5 cut(s) 113, 143, 145, 157, 263
Hpy8I GTNNAC 2 cut(s) 201, 344
Hpy99I CGWCG 1 cut(s) 322
HpyAV CCTTC 2 cut(s) 16, 142
HpyCH4III ACNGT 3 cut(s) 198, 205, 502
HpyCH4V TGCA 3 cut(s) 237, 313, 390
HpyF10VI GCNNNNNNNGC 1 cut(s) 322
Hsp92II CATG 3 cut(s) 308, 434, 448
HspAI GCGC 1 cut(s) 85
KroI GCCGGC 1 cut(s) 215
KroNI GCCGGC 1 cut(s) 217
Kzo9I GATC 2 cut(s) 153, 406
LmnI GCTCC 4 cut(s) 44, 110, 322, 330
Lsp1109I GCAGC 2 cut(s) 72, 325
LweI GCATC 3 cut(s) 148, 445, 531
MaeI CTAG 2 cut(s) 347, 371
MaeIII GTNAC 1 cut(s) 377
MalI GATC 2 cut(s) 155, 408
MboI GATC 2 cut(s) 153, 406
MhlI GDGCHC 2 cut(s) 41, 327
MluCI AATT 6 cut(s) 238, 419, 470, 476, 490, 506
MlyI GAGTC 1 cut(s) 166
MmeI TCCRAC 1 cut(s) 300
MnlI CCTC 9 cut(s) 87, 202, 270, 301, 368, 374, 441, 525, 528
MroNI GCCGGC 1 cut(s) 215
MspI CCGG 1 cut(s) 216
MspR9I CCNGG 2 cut(s) 81, 339
MvaI CCWGG 2 cut(s) 81, 339
MwoI GCNNNNNNNGC 1 cut(s) 322
NaeI GCCGGC 1 cut(s) 217
NdeII GATC 2 cut(s) 153, 406
NgoMIV GCCGGC 1 cut(s) 215
NlaIII CATG 3 cut(s) 308, 434, 448
NlaIV GGNNCC 2 cut(s) 40, 106
PaeR7I CTCGAG 1 cut(s) 143
PdiI GCCGGC 1 cut(s) 217
PfeI GAWTC 1 cut(s) 266
PflMI CCANNNNNTGG 1 cut(s) 17
PkrI GCNGC 2 cut(s) 62, 315
PleI GAGTC 1 cut(s) 166
PpsI GAGTC 1 cut(s) 166
PsiI TTATAA 1 cut(s) 426
Psp124BI GAGCTC 1 cut(s) 327
Psp6I CCWGG 2 cut(s) 79, 337
PspGI CCWGG 2 cut(s) 79, 337
PspN4I GGNNCC 2 cut(s) 40, 106
SacI GAGCTC 1 cut(s) 327
SatI GCNGC 2 cut(s) 61, 314
Sau3AI GATC 2 cut(s) 153, 406
SchI GAGTC 1 cut(s) 166
ScrFI CCNGG 2 cut(s) 81, 339
SduI GDGCHC 2 cut(s) 41, 327
SetI ASST 9 cut(s) 8, 121, 213, 312, 327, 379, 415, 452, 564
SfaNI GCATC 3 cut(s) 148, 445, 531
Sfr274I CTCGAG 1 cut(s) 143
SlaI CTCGAG 1 cut(s) 143
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
Sse9I AATT 6 cut(s) 238, 419, 470, 476, 490, 506
SsiI CCGC 1 cut(s) 27
SspMI CTAG 2 cut(s) 347, 371
SstI GAGCTC 1 cut(s) 327
StyD4I CCNGG 2 cut(s) 79, 337
StyI CCWWGG 1 cut(s) 181
TaaI ACNGT 3 cut(s) 198, 205, 502
TaqI TCGA 2 cut(s) 112, 144
TasI AATT 6 cut(s) 238, 419, 470, 476, 490, 506
TfiI GAWTC 1 cut(s) 266
TscAI CASTG 1 cut(s) 505
TseI GCWGC 2 cut(s) 60, 313
TspDTI ATGAA 3 cut(s) 258, 293, 455
TspRI CASTG 1 cut(s) 505
Van91I CCANNNNNTGG 1 cut(s) 17
XapI RAATTY 1 cut(s) 476
XhoI CTCGAG 1 cut(s) 143
XspI CTAG 2 cut(s) 347, 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.