Rw2G006400

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000515
Physical Location & Seq
Forward (+)
6154806 .. 6156627
1822 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G006400.1

Sequence Viewer

Length: 816 bp
ATGGTTGCTTTTGTGAATTGGACTGTTTTAGTGAGCCAGCCAGAGAAAGTAGTAATAACTAACAAACATGGTGAAAAGCTTGCGGGATTATTGCATGAAACCGGTTCTAAAGAGCTTGTGATCTTGTGCCATGGAGCTCGATCCCACAAGGGAGATAATGTTATGACTAAACTTGCCCTTGCATTGGAAAAGGAAGGAATTAGTATTTTCCGTTTCGATTTTGCTGGAAATGGAGAAAGTGAAGGCACCTTTCAGTTTGGTCAATACTGGAGAGAGGTTGATGACTTGCATGCTGTAGTCCAACACTTCTCTGGGACAAACCATACAGTAATTGCAATTCTTGGCCACAGTAAAGGAGGGGATGAGGTGCTTCTGTATGCTTCTAAGTATGGTGACGTTCGCACGGTTGTCAATGTTTCTGGACGTTATGATCTGAATGGAGGCCTTGAGCTACGGTTAGGGAAAAACTTTATGGAAAGGATCAAGAAGCAAGGATACTTGGATATTAAGGACAAGACAGGAACTTTCGATTGTCGTGTGACGGAGGAAAGTTTGCTGGACCGCATGAATACTAAAATGCATGAATCATGCCTTAAGATTGCCAAAGATTGCCGTGTTTTGACAATCCATGGAACTGATGACGACGTGATCCCCGTTGGAGATGCACTGGAGTTTGCCAAGATAATACCTAACCACAAGTTACATGTTATACAAGGAGCTGATCATTATTACAGCGATCACCAAGCTGAGTTAGCGTCAACTATTTTGGAATTCCTTAAGGCAGCTTTCCAAGAGGACAAGACTACTTCCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

271

Amino Acids

30.2

Weight (kDa)

6.05

Isoelectric Point (pI)

21.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 37 - 242 2.3e-14 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 39 - 141 4.7e-06 alpha/beta hydrolase fold
Peptidase_S9 PF00326 91 - 264 5.3e-06 Prolyl oligopeptidase family
BAAT_C PF08840 104 - 218 4.1e-06 BAAT / Acyl-CoA thioester hydrolase C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47560 AT3G47590 AT3G47590
fragaria_vesca FvH4_1g07650 FvH4_1g07670 FvH4_1g07671 FvH4_1g07680 FvH4_1g07690 FvH4_1g07700 FvH4_1g07700
malus_domestica MD02G1080900.v1.1 MD02G1081000.v1.1 MD02G1081300.v1.1 MD02G1081400.v1.1 MD02G1094000.v1.1 MD15G1208700.v1.1
prunus_persica Prupe.7G208200_v2.0.a1 Prupe.7G208200_v2.0.a1
pyrus_communis pycom02g07440 pycom15g18480
rosa_chinensis RchiOBHm_Chr2g0093511 RchiOBHm_Chr2g0093521 RchiOBHm_Chr2g0093531 RchiOBHm_Chr2g0093541 RchiOBHm_Chr2g0093551 RchiOBHm_Chr2g0093561
rosa_laevigata RLG00000016413 RLG00000016414 RLG00000016415 RLG00000016416 RLG00000016417
rosa_multiflora Rmu_sc0004423.1_g000003 Rmu_sc0004423.1_g000004 Rmu_sc0004423.1_g000005 Rmu_sc0004423.1_g000006 Rmu_sc0006964.1_g000003 Rmu_sc0039672.1_g000001 Rmu_sc0039672.1_g000003
rosa_roxburghii Rroxscaffold_2G00147990 Rroxscaffold_2G00148000 Rroxscaffold_2G00148010 Rroxscaffold_2G00148020 Rroxscaffold_4G00313540
rosa_rugosa Rorug01G0140600.1 Rorug01G0140700.1 Rorug02G0037000 Rorug02G0037100 Rorug02G0037200 Rorug02G0037300 Rorug02G0037400 Rorug02G0037500 Rorug02G0037600
rosa_samantha Rh2AG083400 Rh2AG083500 Rh2AG083700 Rh2AG083800 Rh2AG083900 Rh2AG084000 Rh2BG084200 Rh2BG084300 Rh2BG084400 Rh2BG084500 Rh2BG084600 Rh2CG086300 Rh2CG086400 Rh2CG086500 Rh2CG086700 Rh2CG086800 Rh2CG086900 Rh2DG082200 Rh2DG082300 Rh2DG082400 Rh2DG082500 Rh2DG082600 Rh2DG082700
rosa_wichuraiana Rw1G013200 Rw2G006360 Rw2G006370 Rw2G006380 Rw2G006390 Rw2G006400 Rw2G007130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 245
AciI CCGC 2 cut(s) 83, 562
AclWI GGATC 3 cut(s) 135, 488, 643
AcoI YGGCCR 1 cut(s) 343
AcsI RAATTY 1 cut(s) 770
AfiI CCNNNNNNNGG 1 cut(s) 184
AflII CTTAAG 2 cut(s) 593, 776
AflIII ACRYGT 1 cut(s) 703
AgeI ACCGGT 1 cut(s) 101
AjiI CACGTC 1 cut(s) 646
AluBI AGCT 7 cut(s) 79, 115, 137, 451, 719, 746, 785
AluI AGCT 7 cut(s) 79, 115, 137, 451, 719, 746, 785
Alw21I GWGCWC 1 cut(s) 139
AlwI GGATC 3 cut(s) 135, 488, 643
AoxI GGCC 2 cut(s) 343, 442
ApeKI GCWGC 1 cut(s) 782
ApoI RAATTY 1 cut(s) 770
AsiGI ACCGGT 1 cut(s) 101
AspS9I GGNCC 1 cut(s) 559
AsuHPI GGTGA 3 cut(s) 83, 404, 731
AvaII GGWCC 1 cut(s) 559
BalI TGGCCA 1 cut(s) 345
BanI GGYRCC 1 cut(s) 245
BanII GRGCYC 1 cut(s) 139
BarI GAAGNNNNNNTAC 2 cut(s) 479, 511
Bbv12I GWGCWC 1 cut(s) 139
BbvI GCAGC 1 cut(s) 794
BceAI ACGGC 1 cut(s) 597
BciVI GTATCC 1 cut(s) 488
BclI TGATCA 1 cut(s) 721
BfaI CTAG 1 cut(s) 814
BfmI CTRYAG 1 cut(s) 294
BfrI CTTAAG 2 cut(s) 593, 776
BfuI GTATCC 1 cut(s) 488
BisI GCNGC 1 cut(s) 783
BlsI GCNGC 1 cut(s) 784
Bme18I GGWCC 1 cut(s) 559
BmgBI CACGTC 1 cut(s) 646
BmgT120I GGNCC 1 cut(s) 559
BmiI GGNNCC 1 cut(s) 247
BmsI GCATC 1 cut(s) 652
BpmI CTGGAG 2 cut(s) 289, 689
BpuEI CTTGAG 1 cut(s) 467
BsaJI CCNNGG 2 cut(s) 130, 628
BsaWI WCCGGW 1 cut(s) 101
BsaXI ACNNNNNCTCC 2 cut(s) 144, 174
Bsc4I CCNNNNNNNGG 1 cut(s) 184
Bse118I RCCGGY 1 cut(s) 101
Bse1I ACTGG 2 cut(s) 272, 672
BseDI CCNNGG 2 cut(s) 130, 628
BseGI GGATG 1 cut(s) 367
BseLI CCNNNNNNNGG 1 cut(s) 184
BseMII CTCAG 1 cut(s) 738
BseNI ACTGG 2 cut(s) 272, 672
BseXI GCAGC 1 cut(s) 794
BshFI GGCC 2 cut(s) 345, 444
BshNI GGYRCC 1 cut(s) 245
BshTI ACCGGT 1 cut(s) 101
BsiHKAI GWGCWC 1 cut(s) 139
BsiSI CCGG 1 cut(s) 102
BslFI GGGAC 1 cut(s) 328
BslI CCNNNNNNNGG 1 cut(s) 184
BsmFI GGGAC 1 cut(s) 328
BsnI GGCC 2 cut(s) 345, 444
Bsp1286I GDGCHC 1 cut(s) 139
Bsp143I GATC 7 cut(s) 120, 140, 430, 480, 648, 721, 736
Bsp19I CCATGG 2 cut(s) 130, 628
BspACI CCGC 2 cut(s) 83, 562
BspANI GGCC 2 cut(s) 345, 444
BspCNI CTCAG 1 cut(s) 739
BspLI GGNNCC 1 cut(s) 247
BspPI GGATC 3 cut(s) 135, 488, 643
BspT107I GGYRCC 1 cut(s) 245
BspTI CTTAAG 2 cut(s) 593, 776
BsrFI RCCGGY 1 cut(s) 101
BsrI ACTGG 2 cut(s) 272, 672
BssAI RCCGGY 1 cut(s) 101
BssECI CCNNGG 2 cut(s) 130, 628
BssMI GATC 7 cut(s) 120, 140, 430, 480, 648, 721, 736
BssT1I CCWWGG 2 cut(s) 130, 628
Bst4CI ACNGT 5 cut(s) 25, 328, 350, 406, 456
BstAFI CTTAAG 2 cut(s) 593, 776
BstC8I GCNNGC 3 cut(s) 38, 81, 291
BstDEI CTNAG 2 cut(s) 384, 747
BstDSI CCRYGG 2 cut(s) 130, 628
BstF5I GGATG 1 cut(s) 367
BstKTI GATC 7 cut(s) 123, 143, 433, 483, 651, 724, 739
BstMBI GATC 7 cut(s) 120, 140, 430, 480, 648, 721, 736
BstMWI GCNNNNNNNGC 1 cut(s) 752
BstNSI RCATGY 2 cut(s) 293, 707
BstSFI CTRYAG 1 cut(s) 294
BstV1I GCAGC 1 cut(s) 794
BsuI GTATCC 1 cut(s) 488
BsuRI GGCC 2 cut(s) 345, 444
BtgI CCRYGG 2 cut(s) 130, 628
BtrI CACGTC 1 cut(s) 646
BtsCI GGATG 1 cut(s) 367
BtsIMutI CAGTG 1 cut(s) 665
Cac8I GCNNGC 3 cut(s) 38, 81, 291
Cfr10I RCCGGY 1 cut(s) 101
Cfr13I GGNCC 1 cut(s) 559
CseI GACGC 1 cut(s) 744
CspAI ACCGGT 1 cut(s) 101
CviAII CATG 9 cut(s) 68, 95, 131, 290, 565, 581, 588, 629, 704
DdeI CTNAG 2 cut(s) 384, 747
DpnI GATC 7 cut(s) 122, 142, 432, 482, 650, 723, 738
DpnII GATC 7 cut(s) 120, 140, 430, 480, 648, 721, 736
EaeI YGGCCR 1 cut(s) 343
Ecl136II GAGCTC 1 cut(s) 137
Eco130I CCWWGG 2 cut(s) 130, 628
Eco147I AGGCCT 1 cut(s) 444
Eco24I GRGCYC 1 cut(s) 139
Eco47I GGWCC 1 cut(s) 559
Eco53kI GAGCTC 1 cut(s) 137
EcoICRI GAGCTC 1 cut(s) 137
EcoRI GAATTC 1 cut(s) 770
EcoT14I CCWWGG 2 cut(s) 130, 628
EcoT22I ATGCAT 1 cut(s) 582
EcoT38I GRGCYC 1 cut(s) 139
ErhI CCWWGG 2 cut(s) 130, 628
FaeI CATG 9 cut(s) 71, 98, 134, 293, 568, 584, 591, 632, 707
FaqI GGGAC 1 cut(s) 328
FatI CATG 9 cut(s) 67, 94, 130, 289, 564, 580, 587, 628, 703
FauI CCCGC 1 cut(s) 76
FbaI TGATCA 1 cut(s) 721
Fnu4HI GCNGC 1 cut(s) 783
FokI GGATG 1 cut(s) 374
FriOI GRGCYC 1 cut(s) 139
Fsp4HI GCNGC 1 cut(s) 783
FspBI CTAG 1 cut(s) 814
GluI GCNGC 1 cut(s) 783
GsuI CTGGAG 2 cut(s) 289, 689
HaeIII GGCC 2 cut(s) 345, 444
HapII CCGG 1 cut(s) 102
HgaI GACGC 1 cut(s) 744
Hin1II CATG 9 cut(s) 71, 98, 134, 293, 568, 584, 591, 632, 707
HincII GTYRAC 1 cut(s) 759
HindII GTYRAC 1 cut(s) 759
HindIII AAGCTT 1 cut(s) 77
HinfI GANTC 1 cut(s) 584
HpaII CCGG 1 cut(s) 102
HphI GGTGA 3 cut(s) 83, 404, 731
Hpy166II GTNNAC 1 cut(s) 759
Hpy188I TCNGA 1 cut(s) 435
Hpy188III TCNNGA 2 cut(s) 420, 484
Hpy8I GTNNAC 1 cut(s) 759
Hpy99I CGWCG 1 cut(s) 647
HpyAV CCTTC 2 cut(s) 188, 236
HpyCH4III ACNGT 5 cut(s) 25, 328, 350, 406, 456
HpyCH4IV ACGT 3 cut(s) 396, 424, 645
HpyCH4V TGCA 6 cut(s) 94, 182, 289, 335, 580, 665
HpyF10VI GCNNNNNNNGC 1 cut(s) 752
HpyF3I CTNAG 2 cut(s) 384, 747
HpySE526I ACGT 3 cut(s) 396, 424, 645
Hsp92II CATG 9 cut(s) 71, 98, 134, 293, 568, 584, 591, 632, 707
Ksp22I TGATCA 1 cut(s) 721
Kzo9I GATC 7 cut(s) 120, 140, 430, 480, 648, 721, 736
LmnI GCTCC 2 cut(s) 134, 716
Lsp1109I GCAGC 1 cut(s) 794
LweI GCATC 1 cut(s) 652
MaeI CTAG 1 cut(s) 814
MaeII ACGT 3 cut(s) 396, 424, 645
MaeIII GTNAC 3 cut(s) 392, 538, 699
MalI GATC 7 cut(s) 122, 142, 432, 482, 650, 723, 738
MboI GATC 7 cut(s) 120, 140, 430, 480, 648, 721, 736
MhlI GDGCHC 1 cut(s) 139
MlsI TGGCCA 1 cut(s) 345
MluCI AATT 5 cut(s) 16, 198, 330, 336, 770
MluNI TGGCCA 1 cut(s) 345
MmeI TCCRAC 2 cut(s) 325, 637
MnlI CCTC 6 cut(s) 268, 350, 358, 434, 538, 787
Mox20I TGGCCA 1 cut(s) 345
Mph1103I ATGCAT 1 cut(s) 582
MscI TGGCCA 1 cut(s) 345
MseI TTAA 3 cut(s) 507, 594, 777
Msp20I TGGCCA 1 cut(s) 345
MspCI CTTAAG 2 cut(s) 593, 776
MspI CCGG 1 cut(s) 102
MwoI GCNNNNNNNGC 1 cut(s) 752
NcoI CCATGG 2 cut(s) 130, 628
NdeII GATC 7 cut(s) 120, 140, 430, 480, 648, 721, 736
NlaIII CATG 9 cut(s) 71, 98, 134, 293, 568, 584, 591, 632, 707
NlaIV GGNNCC 1 cut(s) 247
NmuCI GTSAC 2 cut(s) 392, 538
NsiI ATGCAT 1 cut(s) 582
NspI RCATGY 2 cut(s) 293, 707
PaeI GCATGC 1 cut(s) 293
PceI AGGCCT 1 cut(s) 444
PciI ACATGT 1 cut(s) 703
PcsI WCGNNNNNNNCGW 1 cut(s) 651
PfeI GAWTC 1 cut(s) 584
PinAI ACCGGT 1 cut(s) 101
PkrI GCNGC 1 cut(s) 784
PscI ACATGT 1 cut(s) 703
Psp124BI GAGCTC 1 cut(s) 139
PspN4I GGNNCC 1 cut(s) 247
PspPI GGNCC 1 cut(s) 559
SacI GAGCTC 1 cut(s) 139
SaqAI TTAA 3 cut(s) 507, 594, 777
SatI GCNGC 1 cut(s) 783
Sau3AI GATC 7 cut(s) 120, 140, 430, 480, 648, 721, 736
Sau96I GGNCC 1 cut(s) 559
SduI GDGCHC 1 cut(s) 139
SfaNI GCATC 1 cut(s) 652
SfcI CTRYAG 1 cut(s) 294
SinI GGWCC 1 cut(s) 559
SmlI CTYRAG 3 cut(s) 446, 593, 776
SmoI CTYRAG 3 cut(s) 446, 593, 776
SphI GCATGC 1 cut(s) 293
Sse9I AATT 5 cut(s) 16, 198, 330, 336, 770
SseBI AGGCCT 1 cut(s) 444
SsiI CCGC 2 cut(s) 83, 562
SspMI CTAG 1 cut(s) 814
SstI GAGCTC 1 cut(s) 139
StuI AGGCCT 1 cut(s) 444
StyI CCWWGG 2 cut(s) 130, 628
TaaI ACNGT 5 cut(s) 25, 328, 350, 406, 456
TaiI ACGT 3 cut(s) 399, 427, 648
TaqI TCGA 3 cut(s) 139, 216, 528
TasI AATT 5 cut(s) 16, 198, 330, 336, 770
TfiI GAWTC 1 cut(s) 584
Tru1I TTAA 3 cut(s) 507, 594, 777
Tru9I TTAA 3 cut(s) 507, 594, 777
TscAI CASTG 1 cut(s) 672
TseFI GTSAC 2 cut(s) 392, 538
TseI GCWGC 1 cut(s) 782
Tsp45I GTSAC 2 cut(s) 392, 538
TspDTI ATGAA 3 cut(s) 111, 581, 597
TspGWI ACGGA 2 cut(s) 200, 557
TspRI CASTG 1 cut(s) 672
Vha464I CTTAAG 2 cut(s) 593, 776
VpaK11BI GGWCC 1 cut(s) 559
XapI RAATTY 1 cut(s) 770
XceI RCATGY 2 cut(s) 293, 707
XcmI CCANNNNNNNNNTGG 1 cut(s) 308
XspI CTAG 1 cut(s) 814
Zsp2I ATGCAT 1 cut(s) 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.