FvH4_1g08720
MYB Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000883
Physical Location & Seq
Forward (+)
4639682 .. 4642568
2887 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g08720.t1

Sequence Viewer

Length: 1638 bp
ATGGATAGAGAGTCATTCCATAAATTGTGTCAAATTCTTACAACTAAAGGGCAACTACAAGGGACTAGGAATATGTCAGTAGAAGAGATGGTCGCAATGTTTTTAAACATTCTTGCACACCACCATAAAAATCGTGTCATAAAATTCGATTTTCATAGGTCTGGTCGAACCGTTAGCAAATATTTTCATGAGTGTCTAAAAGCAATCATTCGATGTCAAAAAGACTTTTGGAAAACCCCTGAACCCATACCTGAGAACTCAACGGATTATCGGTGGAAGTGGTTTAAGAACTGCTTAGGAGCATTAGATGGAACATATATTAGAGTGCGTGTATCCGAGAAGGACAAACCGAGGTATAGGACAAGGAAAAATGAAATAACAACAAATGTGTTAGGAGTTTGCTCTCCAGAATTGCAATTCATATATGTTTTGGCGGGTTGGGAGGGTTCGGCACATGATGGTCGAGTCCTAAGAGATGCTATAACTAGGAGAAACGGATTAAAAGTTCCAAATGGTAATTATTATTTAGTTGATGGTGGCTATACTAATGGGAAAGGTTTCCTTGCACCATTTAGAGGACAACGTTATCATCTTAATGAGTGGAGAGATAGACACCGACCGACATCAGCACAAGAGTTTTTCAACATGAAACATTCAAGTGCTAGGAATGTCATTGAAAGGATGAGTTCCACAGCAACTTCTCGAGGAAGAGGCCAAAACAAACATTACTGGACAGAAGAGGAAGATGAAAAGCTGGTGGAGTCGCTTCTTGAATTATCCCATGACAAAAAGTGGAAGGCTGATACTAGTTTCAAGAATGGTTATCTCAAGCATTTACAAATTGTGATGGAAGCTAAACTTCCGGGTCATGGAATATTAGCAAACCCACATATTGAGTCCCGTATTAAGACTTTGAAGGCTAAGTATGCTGCTTTGGCAGAGATGCTAAACCAAAGCGGATTTAGCTGGAACGAACAAGAAATGATGTTAGTATGCGAGCAAAGTGTATTTACTGAGTGGGTAGAGAAAAGAAACAAAGATGCTGCTGGGTTATATGGTAAGCCATTTCGGCATTACTATAATCTTGGAGAGATATATGGAAGAGATCGTGCAAATGGACAAAATGTGGGAAATGCTGATGATGATGAAGAGGAAATTAGACGTGAGAATACCAACGTTGATCCATTAGAGGATGAGACTCTCTTTGACAATGTGAACCAAAGTGCAAATATGGAACCACAACATGAAGGGTCTGAAGATGTTGATGTATCCTTTACACAGCCTAGTCCTCAAACCCCAAGTGTCTCGCAACAGATTCCTTCACAAAATGTTGCTTCCAGCAATAGTCGTCGGAAAGGAAAAGCATTGGATGAAATGAGCAAGAACTTTTCTCTCATGGCAAAAGTTGTGGCTGGAATGGCACCTAAGCTTGATGGGTTGGTTAATGTTTTATCTACAGACAAAGATTTATCTGAGTTACAATCTAAACTTGATGGTGAGCTGAGCAAGATGAATTTCCTGACTCCAGTGCAAGTGTTTCATGTCACTAACATCCTGGCACAAAAACATGATCTCCTTAGAGTGTTCTTTACTATGACTGATGAAAGGAAAGAATCATATGTCCTTAACCTGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

546

Amino Acids

62.77

Weight (kDa)

8.48

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 1 - 70 2.2e-21 Domain of unknown function (DUF8040)
DDE_Tnp_4 PF13359 102 - 229 6.3e-17 DDE superfamily endonuclease
Myb_DNA-bind_3 PF12776 243 - 337 1.3e-09 Myb/SANT-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1420
AciI CCGC 2 cut(s) 434, 957
AclI AACGTT 2 cut(s) 583, 1176
AclWI GGATC 1 cut(s) 1175
AcsI RAATTY 3 cut(s) 33, 143, 1513
AcuI CTGAAG 1 cut(s) 1275
AfiI CCNNNNNNNGG 2 cut(s) 575, 869
AgsI TTSAA 6 cut(s) 643, 657, 677, 773, 814, 916
AhlI ACTAGT 1 cut(s) 806
AjiI CACGTC 1 cut(s) 1163
AjnI CCWGG 1 cut(s) 1554
AloI GAACNNNNNNTCC 2 cut(s) 489, 521
AluBI AGCT 5 cut(s) 754, 854, 966, 1429, 1501
AluI AGCT 5 cut(s) 754, 854, 966, 1429, 1501
Alw26I GTCTC 2 cut(s) 1190, 1309
AlwI GGATC 1 cut(s) 1175
Ama87I CYCGRG 1 cut(s) 702
AoxI GGCC 1 cut(s) 712
ApeKI GCWGC 2 cut(s) 929, 1043
ApoI RAATTY 3 cut(s) 33, 143, 1513
Asp700I GAANNNNTTC 1 cut(s) 557
AsuC2I CCSGG 1 cut(s) 864
AsuHPI GGTGA 1 cut(s) 1508
AvaI CYCGRG 1 cut(s) 702
BanI GGYRCC 1 cut(s) 1420
BbvI GCAGC 2 cut(s) 916, 1030
BccI CCATC 7 cut(s) 82, 302, 452, 527, 841, 1427, 1487
BciT130I CCWGG 1 cut(s) 1556
BciVI GTATCC 2 cut(s) 343, 1279
BcnI CCSGG 1 cut(s) 864
BcoDI GTCTC 2 cut(s) 1190, 1309
BcuI ACTAGT 1 cut(s) 806
BfaI CTAG 5 cut(s) 66, 486, 663, 807, 1284
BfmI CTRYAG 1 cut(s) 1455
BfuI GTATCC 2 cut(s) 343, 1279
BglI GCCNNNNNGGC 1 cut(s) 1069
BisI GCNGC 2 cut(s) 930, 1044
BlpI GCTNAGC 1 cut(s) 1502
BlsI GCNGC 2 cut(s) 931, 1045
Bme1390I CCNGG 2 cut(s) 864, 1556
BmeT110I CYCGRG 1 cut(s) 702
BmgBI CACGTC 1 cut(s) 1163
BmiI GGNNCC 2 cut(s) 1236, 1422
BmrFI CCNGG 2 cut(s) 864, 1556
BmsI GCATC 3 cut(s) 466, 933, 1030
BpmI CTGGAG 2 cut(s) 390, 1509
Bpu10I CCTNAGC 2 cut(s) 295, 1425
Bpu1102I GCTNAGC 1 cut(s) 1502
BpuEI CTTGAG 1 cut(s) 812
BpuMI CCSGG 1 cut(s) 864
BsaJI CCNNGG 1 cut(s) 350
BsaXI ACNNNNNCTCC 2 cut(s) 1557, 1587
Bsc4I CCNNNNNNNGG 2 cut(s) 575, 869
Bse1I ACTGG 2 cut(s) 734, 1526
Bse3DI GCAATG 1 cut(s) 102
BseBI CCWGG 1 cut(s) 1556
BseDI CCNNGG 1 cut(s) 350
BseGI GGATG 4 cut(s) 687, 1198, 1375, 1551
BseLI CCNNNNNNNGG 2 cut(s) 575, 869
BseMI GCAATG 1 cut(s) 102
BseMII CTCAG 4 cut(s) 243, 1005, 1464, 1493
BseNI ACTGG 2 cut(s) 734, 1526
BseXI GCAGC 2 cut(s) 916, 1030
BseYI CCCAGC 1 cut(s) 1046
Bsh1285I CGRYCG 1 cut(s) 620
BshFI GGCC 1 cut(s) 714
BshNI GGYRCC 1 cut(s) 1420
BsiEI CGRYCG 1 cut(s) 620
BsiHKCI CYCGRG 1 cut(s) 702
BsiSI CCGG 1 cut(s) 863
BslFI GGGAC 2 cut(s) 76, 883
BslI CCNNNNNNNGG 2 cut(s) 575, 869
BsmAI GTCTC 2 cut(s) 1190, 1309
BsmFI GGGAC 2 cut(s) 76, 883
BsnI GGCC 1 cut(s) 714
BsoBI CYCGRG 1 cut(s) 702
Bsp143I GATC 3 cut(s) 1105, 1180, 1570
Bsp1720I GCTNAGC 1 cut(s) 1502
BspACI CCGC 2 cut(s) 434, 957
BspANI GGCC 1 cut(s) 714
BspCNI CTCAG 4 cut(s) 244, 1006, 1465, 1494
BspHI TCATGA 1 cut(s) 187
BspLI GGNNCC 2 cut(s) 1236, 1422
BspPI GGATC 1 cut(s) 1175
BspT107I GGYRCC 1 cut(s) 1420
BsrDI GCAATG 1 cut(s) 102
BsrI ACTGG 2 cut(s) 734, 1526
BssECI CCNNGG 1 cut(s) 350
BssMI GATC 3 cut(s) 1105, 1180, 1570
Bst2UI CCWGG 1 cut(s) 1556
Bst4CI ACNGT 1 cut(s) 172
Bst6I CTCTTC 5 cut(s) 78, 703, 732, 1096, 1143
BstC8I GCNNGC 1 cut(s) 998
BstDEI CTNAG 9 cut(s) 252, 295, 470, 921, 1014, 1425, 1473, 1502, 1577
BstF5I GGATG 4 cut(s) 687, 1198, 1375, 1551
BstKTI GATC 3 cut(s) 1108, 1183, 1573
BstMAI GTCTC 2 cut(s) 1190, 1309
BstMBI GATC 3 cut(s) 1105, 1180, 1570
BstMCI CGRYCG 1 cut(s) 620
BstMWI GCNNNNNNNGC 5 cut(s) 926, 935, 963, 1069, 1418
BstNI CCWGG 1 cut(s) 1556
BstSCI CCNGG 2 cut(s) 862, 1554
BstSFI CTRYAG 1 cut(s) 1455
BstV1I GCAGC 2 cut(s) 916, 1030
BsuI GTATCC 2 cut(s) 343, 1279
BsuRI GGCC 1 cut(s) 714
BtrI CACGTC 1 cut(s) 1163
BtsCI GGATG 4 cut(s) 687, 1198, 1375, 1551
BtsIMutI CAGTG 1 cut(s) 1533
Cac8I GCNNGC 1 cut(s) 998
CciI TCATGA 1 cut(s) 187
CspCI CAANNNNNGTGG 2 cut(s) 1389, 1424
CviAII CATG 9 cut(s) 188, 455, 646, 782, 869, 1244, 1396, 1541, 1568
DdeI CTNAG 9 cut(s) 252, 295, 470, 921, 1014, 1425, 1473, 1502, 1577
DpnI GATC 3 cut(s) 1107, 1182, 1572
DpnII GATC 3 cut(s) 1105, 1180, 1570
DraI TTTAAA 1 cut(s) 105
Eam1104I CTCTTC 5 cut(s) 78, 703, 732, 1096, 1143
EarI CTCTTC 5 cut(s) 78, 703, 732, 1096, 1143
Eco57I CTGAAG 1 cut(s) 1275
Eco88I CYCGRG 1 cut(s) 702
EcoRII CCWGG 1 cut(s) 1554
FaeI CATG 9 cut(s) 191, 458, 649, 785, 872, 1247, 1399, 1544, 1571
FalI AAGNNNNNCTT 6 cut(s) 278, 310, 546, 578, 843, 875
FaqI GGGAC 2 cut(s) 76, 883
FatI CATG 9 cut(s) 187, 454, 645, 781, 868, 1243, 1395, 1540, 1567
FauI CCCGC 1 cut(s) 427
FauNDI CATATG 1 cut(s) 1618
Fnu4HI GCNGC 2 cut(s) 930, 1044
FokI GGATG 4 cut(s) 694, 1205, 1382, 1538
Fsp4HI GCNGC 2 cut(s) 930, 1044
FspBI CTAG 5 cut(s) 66, 486, 663, 807, 1284
GluI GCNGC 2 cut(s) 930, 1044
GsaI CCCAGC 1 cut(s) 1050
GsuI CTGGAG 2 cut(s) 390, 1509
HaeIII GGCC 1 cut(s) 714
HapII CCGG 1 cut(s) 863
Hin1II CATG 9 cut(s) 191, 458, 649, 785, 872, 1247, 1399, 1544, 1571
HindIII AAGCTT 1 cut(s) 1427
HinfI GANTC 8 cut(s) 11, 465, 761, 896, 1198, 1315, 1522, 1613
HpaII CCGG 1 cut(s) 863
HphI GGTGA 1 cut(s) 1508
Hpy166II GTNNAC 1 cut(s) 1216
Hpy188I TCNGA 4 cut(s) 337, 1255, 1353, 1474
Hpy188III TCNNGA 6 cut(s) 188, 407, 702, 770, 814, 1519
Hpy8I GTNNAC 1 cut(s) 1216
Hpy99I CGWCG 1 cut(s) 1353
HpyAV CCTTC 5 cut(s) 334, 790, 910, 1241, 1329
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4IV ACGT 3 cut(s) 583, 1162, 1176
HpyCH4V TGCA 6 cut(s) 116, 415, 566, 1112, 1226, 1531
HpyF10VI GCNNNNNNNGC 5 cut(s) 926, 935, 963, 1069, 1418
HpyF3I CTNAG 9 cut(s) 252, 295, 470, 921, 1014, 1425, 1473, 1502, 1577
HpySE526I ACGT 3 cut(s) 583, 1162, 1176
Hsp92II CATG 9 cut(s) 191, 458, 649, 785, 872, 1247, 1399, 1544, 1571
Kzo9I GATC 3 cut(s) 1105, 1180, 1570
LmnI GCTCC 1 cut(s) 299
Lsp1109I GCAGC 2 cut(s) 916, 1030
LweI GCATC 3 cut(s) 466, 933, 1030
MaeI CTAG 5 cut(s) 66, 486, 663, 807, 1284
MaeII ACGT 3 cut(s) 583, 1162, 1176
MaeIII GTNAC 2 cut(s) 1476, 1543
MalI GATC 3 cut(s) 1107, 1182, 1572
MboI GATC 3 cut(s) 1105, 1180, 1570
MboII GAAGA 7 cut(s) 95, 720, 749, 755, 1113, 1160, 1268
MlyI GAGTC 6 cut(s) 20, 474, 770, 905, 1192, 1516
MmeI TCCRAC 1 cut(s) 1331
MnlI CCTC 9 cut(s) 345, 436, 569, 698, 704, 733, 1144, 1183, 1299
MroXI GAANNNNTTC 1 cut(s) 557
MseI TTAA 7 cut(s) 104, 285, 500, 594, 906, 1443, 1626
MslI CAYNNNNRTG 2 cut(s) 594, 657
MspI CCGG 1 cut(s) 863
MspR9I CCNGG 2 cut(s) 864, 1556
MvaI CCWGG 1 cut(s) 1556
MwoI GCNNNNNNNGC 5 cut(s) 926, 935, 963, 1069, 1418
NciI CCSGG 1 cut(s) 864
NdeI CATATG 1 cut(s) 1618
NdeII GATC 3 cut(s) 1105, 1180, 1570
NlaIII CATG 9 cut(s) 191, 458, 649, 785, 872, 1247, 1399, 1544, 1571
NlaIV GGNNCC 2 cut(s) 1236, 1422
NmuCI GTSAC 1 cut(s) 1543
PaeR7I CTCGAG 1 cut(s) 702
PagI TCATGA 1 cut(s) 187
PdmI GAANNNNTTC 1 cut(s) 557
PfeI GAWTC 2 cut(s) 1315, 1613
PkrI GCNGC 2 cut(s) 931, 1045
PleI GAGTC 6 cut(s) 19, 473, 769, 904, 1192, 1516
PpsI GAGTC 6 cut(s) 19, 473, 769, 904, 1192, 1516
Psp1406I AACGTT 2 cut(s) 583, 1176
Psp6I CCWGG 1 cut(s) 1554
PspFI CCCAGC 1 cut(s) 1046
PspGI CCWGG 1 cut(s) 1554
PspN4I GGNNCC 2 cut(s) 1236, 1422
RseI CAYNNNNRTG 2 cut(s) 594, 657
SaqAI TTAA 7 cut(s) 104, 285, 500, 594, 906, 1443, 1626
SatI GCNGC 2 cut(s) 930, 1044
Sau3AI GATC 3 cut(s) 1105, 1180, 1570
SchI GAGTC 6 cut(s) 20, 474, 770, 905, 1192, 1516
ScrFI CCNGG 2 cut(s) 864, 1556
SfaNI GCATC 3 cut(s) 466, 933, 1030
SfcI CTRYAG 1 cut(s) 1455
Sfr274I CTCGAG 1 cut(s) 702
SlaI CTCGAG 1 cut(s) 702
SmiMI CAYNNNNRTG 2 cut(s) 594, 657
SmlI CTYRAG 2 cut(s) 702, 827
SmoI CTYRAG 2 cut(s) 702, 827
SpeI ACTAGT 1 cut(s) 806
SsiI CCGC 2 cut(s) 434, 957
SspI AATATT 2 cut(s) 182, 876
SspMI CTAG 5 cut(s) 66, 486, 663, 807, 1284
StyD4I CCNGG 2 cut(s) 862, 1554
TaaI ACNGT 1 cut(s) 172
TaiI ACGT 3 cut(s) 586, 1165, 1179
TaqI TCGA 5 cut(s) 147, 166, 211, 463, 703
TaqII GACCGA 1 cut(s) 634
TfiI GAWTC 2 cut(s) 1315, 1613
Tru1I TTAA 7 cut(s) 104, 285, 500, 594, 906, 1443, 1626
Tru9I TTAA 7 cut(s) 104, 285, 500, 594, 906, 1443, 1626
TscAI CASTG 1 cut(s) 1533
TseFI GTSAC 1 cut(s) 1543
TseI GCWGC 2 cut(s) 929, 1043
Tsp45I GTSAC 1 cut(s) 1543
TspGWI ACGGA 2 cut(s) 278, 510
TspRI CASTG 1 cut(s) 1533
XapI RAATTY 3 cut(s) 33, 143, 1513
XhoI CTCGAG 1 cut(s) 702
XmnI GAANNNNTTC 1 cut(s) 557
XspI CTAG 5 cut(s) 66, 486, 663, 807, 1284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.