pycom01g10330

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000883
Physical Location & Seq
Reverse (-)
11140159 .. 11140556
398 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g10330.2

Sequence Viewer

Length: 249 bp
ATGCAGGATTGTGTACCTGTGATCTTTGAAATGTTGTATTGTTGTGGGTTGAGCTCTCTTCACTGGTTGCAACAAAAACTTGACGGTGAGTTGAGCAAAATGGAGTTTTTGGCTCCCATGGAATTATTTAAAGTCACCAACTTCTTGGCTAAAGAACATGACATTCTGAGGGTTTTTTTCAACATGTCTGATGAAAGGAAGAGTGCACATGCGACTACTTTGTTGCAAACTTGGATGCATAATGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

83

Amino Acids

9.65

Weight (kDa)

5.42

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 15
AflIII ACRYGT 1 cut(s) 183
AgsI TTSAA 2 cut(s) 29, 181
AluBI AGCT 1 cut(s) 54
AluI AGCT 1 cut(s) 54
Alw21I GWGCWC 2 cut(s) 56, 208
Alw44I GTGCAC 1 cut(s) 204
ApaLI GTGCAC 1 cut(s) 204
AsuHPI GGTGA 2 cut(s) 98, 127
BaeGI GKGCMC 1 cut(s) 208
BanII GRGCYC 1 cut(s) 56
Bbv12I GWGCWC 2 cut(s) 56, 208
BfaI CTAG 1 cut(s) 247
BmiI GGNNCC 1 cut(s) 114
BmsI GCATC 1 cut(s) 225
BsaJI CCNNGG 1 cut(s) 117
Bse1I ACTGG 1 cut(s) 68
BseDI CCNNGG 1 cut(s) 117
BseGI GGATG 1 cut(s) 240
BseMII CTCAG 1 cut(s) 158
BseNI ACTGG 1 cut(s) 68
BseSI GKGCMC 1 cut(s) 208
BsiHKAI GWGCWC 2 cut(s) 56, 208
Bsp1286I GDGCHC 2 cut(s) 56, 208
Bsp143I GATC 1 cut(s) 21
Bsp19I CCATGG 1 cut(s) 117
BspCNI CTCAG 1 cut(s) 159
BspLI GGNNCC 1 cut(s) 114
BsrI ACTGG 1 cut(s) 68
BssECI CCNNGG 1 cut(s) 117
BssMI GATC 1 cut(s) 21
BssT1I CCWWGG 1 cut(s) 117
Bst4CI ACNGT 1 cut(s) 86
Bst6I CTCTTC 2 cut(s) 63, 194
BstDEI CTNAG 1 cut(s) 167
BstDSI CCRYGG 1 cut(s) 117
BstF5I GGATG 1 cut(s) 240
BstKTI GATC 1 cut(s) 24
BstMBI GATC 1 cut(s) 21
BstNSI RCATGY 2 cut(s) 187, 212
BstSLI GKGCMC 1 cut(s) 208
BstXI CCANNNNNNTGG 1 cut(s) 145
BtgI CCRYGG 1 cut(s) 117
BtsCI GGATG 1 cut(s) 240
BtsIMutI CAGTG 1 cut(s) 61
Csp6I GTAC 1 cut(s) 14
CviAII CATG 4 cut(s) 118, 158, 184, 209
CviJI RGCY 3 cut(s) 54, 113, 149
CviKI_1 RGCY 3 cut(s) 54, 113, 149
CviQI GTAC 1 cut(s) 14
DdeI CTNAG 1 cut(s) 167
DpnI GATC 1 cut(s) 23
DpnII GATC 1 cut(s) 21
DraI TTTAAA 1 cut(s) 130
Eam1104I CTCTTC 2 cut(s) 63, 194
EarI CTCTTC 2 cut(s) 63, 194
Ecl136II GAGCTC 1 cut(s) 54
Eco130I CCWWGG 1 cut(s) 117
Eco24I GRGCYC 1 cut(s) 56
Eco53kI GAGCTC 1 cut(s) 54
EcoICRI GAGCTC 1 cut(s) 54
EcoT14I CCWWGG 1 cut(s) 117
EcoT22I ATGCAT 1 cut(s) 240
EcoT38I GRGCYC 1 cut(s) 56
ErhI CCWWGG 1 cut(s) 117
FaeI CATG 4 cut(s) 121, 161, 187, 212
FaiI YATR 5 cut(s) 119, 159, 185, 210, 240
FatI CATG 4 cut(s) 117, 157, 183, 208
FriOI GRGCYC 1 cut(s) 56
FspBI CTAG 1 cut(s) 247
Hin1II CATG 4 cut(s) 121, 161, 187, 212
HphI GGTGA 2 cut(s) 98, 127
Hpy166II GTNNAC 2 cut(s) 14, 206
Hpy188I TCNGA 2 cut(s) 168, 190
Hpy8I GTNNAC 2 cut(s) 14, 206
HpyCH4III ACNGT 1 cut(s) 86
HpyCH4V TGCA 5 cut(s) 4, 70, 206, 226, 238
HpyF3I CTNAG 1 cut(s) 167
Hsp92II CATG 4 cut(s) 121, 161, 187, 212
Kzo9I GATC 1 cut(s) 21
LmnI GCTCC 1 cut(s) 118
LpnPI CCDG 2 cut(s) 30, 49
LweI GCATC 1 cut(s) 225
MaeI CTAG 1 cut(s) 247
MaeIII GTNAC 1 cut(s) 133
MalI GATC 1 cut(s) 23
MboI GATC 1 cut(s) 21
MboII GAAGA 2 cut(s) 50, 211
MhlI GDGCHC 2 cut(s) 56, 208
MluCI AATT 1 cut(s) 122
MnlI CCTC 1 cut(s) 162
Mph1103I ATGCAT 1 cut(s) 240
MseI TTAA 1 cut(s) 129
NcoI CCATGG 1 cut(s) 117
NdeII GATC 1 cut(s) 21
NlaIII CATG 4 cut(s) 121, 161, 187, 212
NlaIV GGNNCC 1 cut(s) 114
NmuCI GTSAC 1 cut(s) 133
NsiI ATGCAT 1 cut(s) 240
NspI RCATGY 2 cut(s) 187, 212
PciI ACATGT 1 cut(s) 183
PscI ACATGT 1 cut(s) 183
Psp124BI GAGCTC 1 cut(s) 56
PspN4I GGNNCC 1 cut(s) 114
RsaI GTAC 1 cut(s) 15
RsaNI GTAC 1 cut(s) 14
SacI GAGCTC 1 cut(s) 56
SaqAI TTAA 1 cut(s) 129
Sau3AI GATC 1 cut(s) 21
SduI GDGCHC 2 cut(s) 56, 208
SetI ASST 2 cut(s) 19, 56
SfaNI GCATC 1 cut(s) 225
Sse9I AATT 1 cut(s) 122
SspMI CTAG 1 cut(s) 247
SstI GAGCTC 1 cut(s) 56
StyI CCWWGG 1 cut(s) 117
TaaI ACNGT 1 cut(s) 86
TasI AATT 1 cut(s) 122
Tru1I TTAA 1 cut(s) 129
Tru9I TTAA 1 cut(s) 129
TscAI CASTG 1 cut(s) 68
TseFI GTSAC 1 cut(s) 133
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 1 cut(s) 207
TspRI CASTG 1 cut(s) 68
VneI GTGCAC 1 cut(s) 204
XceI RCATGY 2 cut(s) 187, 212
XspI CTAG 1 cut(s) 247
Zsp2I ATGCAT 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.