FvH4_2g10222

Enzymatic polyprotein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001755
Physical Location & Seq
Reverse (-)
9070363 .. 9072013
1651 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g10222.t1

Sequence Viewer

Length: 822 bp
ATGGCTCAGACTAATAGTCCTTTAGATTTTGCAGTGCCTATCTACGCACAATTTTGCGGAGATATCACGGGGCATAGTGAGCAATCTAAAGAAAGATCAAAGGCGAATATTCAAAAATTGAGTATTTGTAACATGAGATGTTGGCTTTGCAAGGCAGAAGGACATTATGCAAATGAATGTCCTAAAAAGGACAAGAGATCTTCTAAAGCTTTAATCGCTGAGTATGATGAAGCAATAGAGTATGCTAATTTAAAAGGCTTTGAAGTTGCATATTCTGATGAAGAGAATGAATCAATATATTCTCTTGAGTACCCATCAGATAGTGAGACAGAAAGTACTTCAGAAAATTCTGAAGAAGAAAATATAGGAGATCGTCCTATATTTTTATTACAGATCCAAAATTGGAAGAAAGAAAACACTGATATTATCAGTACCTTCCATCCCAATCCAGGAAGATTTATTTGTGACTATTGCAAATGTGATGATAATAATACAATTCCCATGTACTGTGAAGGTACAGGTGAAACTTATCATCGGGAATGTTTTATTGCAGAACTCAGAAGAAAAACCAAGGATAATGAAGCACATGTCCTGGTGGAAGAGGAATACAATATACTGGTTCAGGGTTTAGTCTTGCTGAAAGATATGCTATCCCTGAAGAATACTGGGAAAAATCTCCAAGGACAGTTACCGGAGTTGCAATGGAAGAAAACAACATCGTTATGGATACAATGGCACGTAATGTGCAAGTTTTCTTGGGCGGAGGCACTTTTACCATCAAGATTATGTGGCAGTGTGAAGGTCAGACAACTGATTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_A3 RVT_1

Protein Analysis

274

Amino Acids

31.41

Weight (kDa)

5.15

Isoelectric Point (pI)

58.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 57, 761
AclWI GGATC 1 cut(s) 388
AcsI RAATTY 1 cut(s) 346
AcuI CTGAAG 3 cut(s) 324, 372, 677
AfaI GTAC 5 cut(s) 311, 337, 433, 506, 517
AfiI CCNNNNNNNGG 1 cut(s) 449
AflIII ACRYGT 1 cut(s) 586
AgsI TTSAA 2 cut(s) 113, 263
AhdI GACNNNNNGTC 1 cut(s) 15
AjnI CCWGG 2 cut(s) 448, 591
AluBI AGCT 1 cut(s) 209
AluI AGCT 1 cut(s) 209
Alw26I GTCTC 1 cut(s) 320
AlwI GGATC 1 cut(s) 388
ApoI RAATTY 1 cut(s) 346
ArsI GACNNNNNNTTYG 1 cut(s) 798
AsuHPI GGTGA 1 cut(s) 533
BccI CCATC 3 cut(s) 322, 447, 784
BciT130I CCWGG 2 cut(s) 450, 593
BciVI GTATCC 1 cut(s) 720
BcoDI GTCTC 1 cut(s) 320
BfuI GTATCC 1 cut(s) 720
BglII AGATCT 1 cut(s) 197
BmcAI AGTACT 1 cut(s) 337
Bme1390I CCNGG 2 cut(s) 450, 593
BmeRI GACNNNNNGTC 1 cut(s) 15
BmrFI CCNGG 2 cut(s) 450, 593
BmrI ACTGGG 1 cut(s) 675
BmuI ACTGGG 1 cut(s) 675
BpuEI CTTGAG 1 cut(s) 326
BsaAI YACGTR 1 cut(s) 739
BsaJI CCNNGG 2 cut(s) 570, 679
BsaWI WCCGGW 1 cut(s) 691
Bsc4I CCNNNNNNNGG 1 cut(s) 449
Bse1I ACTGG 2 cut(s) 621, 670
Bse3DI GCAATG 1 cut(s) 707
BseBI CCWGG 2 cut(s) 450, 593
BseDI CCNNGG 2 cut(s) 570, 679
BseGI GGATG 1 cut(s) 439
BseLI CCNNNNNNNGG 1 cut(s) 449
BseMI GCAATG 1 cut(s) 707
BseMII CTCAG 3 cut(s) 20, 210, 571
BseNI ACTGG 2 cut(s) 621, 670
BsiSI CCGG 1 cut(s) 692
BslI CCNNNNNNNGG 1 cut(s) 449
BsmAI GTCTC 1 cut(s) 320
Bsp143I GATC 4 cut(s) 95, 197, 370, 393
BspACI CCGC 2 cut(s) 57, 761
BspCNI CTCAG 3 cut(s) 19, 211, 570
BspPI GGATC 1 cut(s) 388
BsrDI GCAATG 1 cut(s) 707
BsrI ACTGG 2 cut(s) 621, 670
BssECI CCNNGG 2 cut(s) 570, 679
BssMI GATC 4 cut(s) 95, 197, 370, 393
BssT1I CCWWGG 2 cut(s) 570, 679
Bst2UI CCWGG 2 cut(s) 450, 593
Bst4CI ACNGT 2 cut(s) 509, 687
Bst6I CTCTTC 2 cut(s) 276, 594
BstBAI YACGTR 1 cut(s) 739
BstDEI CTNAG 3 cut(s) 6, 219, 557
BstF5I GGATG 1 cut(s) 439
BstKTI GATC 4 cut(s) 98, 200, 373, 396
BstMAI GTCTC 1 cut(s) 320
BstMBI GATC 4 cut(s) 95, 197, 370, 393
BstMWI GCNNNNNNNGC 2 cut(s) 79, 215
BstNI CCWGG 2 cut(s) 450, 593
BstNSI RCATGY 1 cut(s) 590
BstSCI CCNGG 2 cut(s) 448, 591
BstX2I RGATCY 2 cut(s) 197, 393
BstYI RGATCY 2 cut(s) 197, 393
BsuI GTATCC 1 cut(s) 720
BtsCI GGATG 1 cut(s) 439
BtsI GCAGTG 2 cut(s) 39, 799
BtsIMutI CAGTG 3 cut(s) 39, 417, 799
Csp6I GTAC 5 cut(s) 310, 336, 432, 505, 516
CviAII CATG 3 cut(s) 133, 502, 587
CviJI RGCY 4 cut(s) 5, 145, 209, 258
CviKI_1 RGCY 4 cut(s) 5, 145, 209, 258
CviQI GTAC 5 cut(s) 310, 336, 432, 505, 516
DdeI CTNAG 3 cut(s) 6, 219, 557
DpnI GATC 4 cut(s) 97, 199, 372, 395
DpnII GATC 4 cut(s) 95, 197, 370, 393
DraI TTTAAA 1 cut(s) 252
DriI GACNNNNNGTC 1 cut(s) 15
Eam1104I CTCTTC 2 cut(s) 276, 594
Eam1105I GACNNNNNGTC 1 cut(s) 15
EarI CTCTTC 2 cut(s) 276, 594
EciI GGCGGA 1 cut(s) 776
Eco130I CCWWGG 2 cut(s) 570, 679
Eco32I GATATC 1 cut(s) 64
Eco57I CTGAAG 3 cut(s) 324, 372, 677
EcoRII CCWGG 2 cut(s) 448, 591
EcoRV GATATC 1 cut(s) 64
EcoT14I CCWWGG 2 cut(s) 570, 679
ErhI CCWWGG 2 cut(s) 570, 679
FaeI CATG 3 cut(s) 136, 505, 590
FatI CATG 3 cut(s) 132, 501, 586
FokI GGATG 1 cut(s) 426
HapII CCGG 1 cut(s) 692
Hin1II CATG 3 cut(s) 136, 505, 590
HindIII AAGCTT 1 cut(s) 207
HinfI GANTC 1 cut(s) 290
HpaII CCGG 1 cut(s) 692
HphI GGTGA 1 cut(s) 533
Hpy188I TCNGA 7 cut(s) 9, 277, 319, 343, 352, 560, 806
Hpy188III TCNNGA 3 cut(s) 305, 536, 780
HpyAV CCTTC 4 cut(s) 152, 445, 506, 793
HpyCH4III ACNGT 2 cut(s) 509, 687
HpyCH4IV ACGT 1 cut(s) 738
HpyCH4V TGCA 8 cut(s) 32, 150, 170, 269, 474, 551, 700, 747
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 215
HpyF3I CTNAG 3 cut(s) 6, 219, 557
HpySE526I ACGT 1 cut(s) 738
Hsp92II CATG 3 cut(s) 136, 505, 590
Kzo9I GATC 4 cut(s) 95, 197, 370, 393
MaeII ACGT 1 cut(s) 738
MaeIII GTNAC 3 cut(s) 128, 464, 687
MalI GATC 4 cut(s) 97, 199, 372, 395
MboI GATC 4 cut(s) 95, 197, 370, 393
MflI RGATCY 2 cut(s) 197, 393
MluCI AATT 6 cut(s) 50, 116, 247, 346, 400, 495
MnlI CCTC 2 cut(s) 595, 757
MseI TTAA 2 cut(s) 212, 251
MslI CAYNNNNRTG 1 cut(s) 721
MspI CCGG 1 cut(s) 692
MspR9I CCNGG 2 cut(s) 450, 593
MvaI CCWGG 2 cut(s) 450, 593
MwoI GCNNNNNNNGC 2 cut(s) 79, 215
NdeII GATC 4 cut(s) 95, 197, 370, 393
NlaIII CATG 3 cut(s) 136, 505, 590
NmuCI GTSAC 1 cut(s) 464
NspI RCATGY 1 cut(s) 590
PciI ACATGT 1 cut(s) 586
PfeI GAWTC 1 cut(s) 290
PfoI TCCNGGA 1 cut(s) 448
Ppu21I YACGTR 1 cut(s) 739
PscI ACATGT 1 cut(s) 586
Psp6I CCWGG 2 cut(s) 448, 591
PspGI CCWGG 2 cut(s) 448, 591
PsuI RGATCY 2 cut(s) 197, 393
RsaI GTAC 5 cut(s) 311, 337, 433, 506, 517
RsaNI GTAC 5 cut(s) 310, 336, 432, 505, 516
RseI CAYNNNNRTG 1 cut(s) 721
SaqAI TTAA 2 cut(s) 212, 251
Sau3AI GATC 4 cut(s) 95, 197, 370, 393
ScaI AGTACT 1 cut(s) 337
ScrFI CCNGG 2 cut(s) 450, 593
SetI ASST 6 cut(s) 211, 437, 517, 523, 741, 804
SmiMI CAYNNNNRTG 1 cut(s) 721
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
Sse9I AATT 6 cut(s) 50, 116, 247, 346, 400, 495
SsiI CCGC 2 cut(s) 57, 761
SspI AATATT 1 cut(s) 109
StyD4I CCNGG 2 cut(s) 448, 591
StyI CCWWGG 2 cut(s) 570, 679
TaaI ACNGT 2 cut(s) 509, 687
TaiI ACGT 1 cut(s) 741
TasI AATT 6 cut(s) 50, 116, 247, 346, 400, 495
TatI WGTACW 2 cut(s) 335, 504
TfiI GAWTC 1 cut(s) 290
Tru1I TTAA 2 cut(s) 212, 251
Tru9I TTAA 2 cut(s) 212, 251
TscAI CASTG 3 cut(s) 39, 424, 799
TseFI GTSAC 1 cut(s) 464
Tsp45I GTSAC 1 cut(s) 464
TspDTI ATGAA 5 cut(s) 189, 243, 294, 303, 594
TspRI CASTG 3 cut(s) 39, 424, 799
XapI RAATTY 1 cut(s) 346
XceI RCATGY 1 cut(s) 590
ZrmI AGTACT 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.