Enzymatic polyprotein-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1199 |
| AccI | GTMKAC | 1 cut(s) | 1182 |
| AccII | CGCG | 1 cut(s) | 1637 |
| AciI | CCGC | 3 cut(s) | 1166, 1364, 1533 |
| AclWI | GGATC | 2 cut(s) | 451, 643 |
| AcsI | RAATTY | 5 cut(s) | 250, 395, 836, 941, 1401 |
| AcuI | CTGAAG | 4 cut(s) | 72, 306, 645, 691 |
| AfaI | GTAC | 2 cut(s) | 920, 1047 |
| AflII | CTTAAG | 1 cut(s) | 1109 |
| AgsI | TTSAA | 7 cut(s) | 98, 257, 266, 338, 986, 1156, 1595 |
| AhlI | ACTAGT | 1 cut(s) | 359 |
| AjnI | CCWGG | 3 cut(s) | 769, 1197, 1678 |
| AluBI | AGCT | 5 cut(s) | 50, 320, 334, 1118, 1255 |
| AluI | AGCT | 5 cut(s) | 50, 320, 334, 1118, 1255 |
| Alw26I | GTCTC | 1 cut(s) | 358 |
| AlwI | GGATC | 2 cut(s) | 451, 643 |
| AoxI | GGCC | 3 cut(s) | 628, 750, 1075 |
| ApoI | RAATTY | 5 cut(s) | 250, 395, 836, 941, 1401 |
| AseI | ATTAAT | 1 cut(s) | 980 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 279, 941, 1295 |
| AspA2I | CCTAGG | 2 cut(s) | 1279, 1324 |
| AspLEI | GCGC | 1 cut(s) | 1678 |
| AspS9I | GGNCC | 2 cut(s) | 767, 1653 |
| AsuHPI | GGTGA | 5 cut(s) | 538, 571, 811, 824, 1522 |
| AvaII | GGWCC | 2 cut(s) | 767, 1653 |
| AvrII | CCTAGG | 2 cut(s) | 1279, 1324 |
| BanII | GRGCYC | 1 cut(s) | 846 |
| BbsI | GAAGAC | 1 cut(s) | 1195 |
| BccI | CCATC | 4 cut(s) | 202, 527, 549, 1135 |
| BciT130I | CCWGG | 3 cut(s) | 771, 1199, 1680 |
| BcoDI | GTCTC | 1 cut(s) | 358 |
| BcuI | ACTAGT | 1 cut(s) | 359 |
| BfaI | CTAG | 7 cut(s) | 360, 479, 996, 1280, 1325, 1421, 1667 |
| BfoI | RGCGCY | 1 cut(s) | 1679 |
| BfrI | CTTAAG | 1 cut(s) | 1109 |
| BglII | AGATCT | 2 cut(s) | 961, 1380 |
| BlnI | CCTAGG | 2 cut(s) | 1279, 1324 |
| Bme1390I | CCNGG | 3 cut(s) | 771, 1199, 1680 |
| Bme18I | GGWCC | 2 cut(s) | 767, 1653 |
| BmgT120I | GGNCC | 2 cut(s) | 767, 1653 |
| BmiI | GGNNCC | 2 cut(s) | 183, 539 |
| BmrFI | CCNGG | 3 cut(s) | 771, 1199, 1680 |
| BmrI | ACTGGG | 1 cut(s) | 778 |
| BmsI | GCATC | 3 cut(s) | 1020, 1042, 1399 |
| BmuI | ACTGGG | 1 cut(s) | 778 |
| BpiI | GAAGAC | 1 cut(s) | 1195 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1033, 1065 |
| BpmI | CTGGAG | 2 cut(s) | 996, 1610 |
| Bpu10I | CCTNAGC | 1 cut(s) | 321 |
| BpuEI | CTTGAG | 1 cut(s) | 1394 |
| Bsa29I | ATCGAT | 2 cut(s) | 222, 237 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1334 |
| BsaJI | CCNNGG | 4 cut(s) | 631, 1279, 1324, 1432 |
| BsaWI | WCCGGW | 1 cut(s) | 1655 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 708, 738 |
| Bse1I | ACTGG | 4 cut(s) | 264, 523, 784, 1655 |
| Bse3DI | GCAATG | 2 cut(s) | 661, 1494 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1334 |
| BseBI | CCWGG | 3 cut(s) | 771, 1199, 1680 |
| BseCI | ATCGAT | 2 cut(s) | 222, 237 |
| BseDI | CCNNGG | 4 cut(s) | 631, 1279, 1324, 1432 |
| BseGI | GGATG | 4 cut(s) | 603, 694, 1033, 1194 |
| BseJI | GATNNNNATC | 1 cut(s) | 1334 |
| BseMI | GCAATG | 2 cut(s) | 661, 1494 |
| BseMII | CTCAG | 3 cut(s) | 335, 929, 1029 |
| BseNI | ACTGG | 4 cut(s) | 264, 523, 784, 1655 |
| BseRI | GAGGAG | 2 cut(s) | 180, 1578 |
| BsgI | GTGCAG | 1 cut(s) | 30 |
| Bsh1236I | CGCG | 1 cut(s) | 1637 |
| BshFI | GGCC | 3 cut(s) | 630, 752, 1077 |
| BshVI | ATCGAT | 2 cut(s) | 222, 237 |
| BsiSI | CCGG | 1 cut(s) | 1656 |
| BsmAI | GTCTC | 1 cut(s) | 358 |
| BsmBI | CGTCTC | 1 cut(s) | 358 |
| BsmI | GAATGC | 3 cut(s) | 44, 892, 1016 |
| BsnI | GGCC | 3 cut(s) | 630, 752, 1077 |
| Bsp1286I | GDGCHC | 1 cut(s) | 846 |
| Bsp143I | GATC | 8 cut(s) | 403, 443, 635, 794, 814, 931, 961, 1380 |
| BspACI | CCGC | 3 cut(s) | 1166, 1364, 1533 |
| BspANI | GGCC | 3 cut(s) | 630, 752, 1077 |
| BspCNI | CTCAG | 3 cut(s) | 334, 928, 1030 |
| BspDI | ATCGAT | 2 cut(s) | 222, 237 |
| BspFNI | CGCG | 1 cut(s) | 1637 |
| BspLI | GGNNCC | 2 cut(s) | 183, 539 |
| BspPI | GGATC | 2 cut(s) | 451, 643 |
| BspTI | CTTAAG | 1 cut(s) | 1109 |
| BsrDI | GCAATG | 2 cut(s) | 661, 1494 |
| BsrI | ACTGG | 4 cut(s) | 264, 523, 784, 1655 |
| BssECI | CCNNGG | 4 cut(s) | 631, 1279, 1324, 1432 |
| BssMI | GATC | 8 cut(s) | 403, 443, 635, 794, 814, 931, 961, 1380 |
| BssT1I | CCWWGG | 4 cut(s) | 631, 1279, 1324, 1432 |
| Bst2UI | CCWGG | 3 cut(s) | 771, 1199, 1680 |
| Bst4CI | ACNGT | 1 cut(s) | 457 |
| Bst6I | CTCTTC | 2 cut(s) | 1596, 1599 |
| BstAFI | CTTAAG | 1 cut(s) | 1109 |
| BstC8I | GCNNGC | 1 cut(s) | 1116 |
| BstDEI | CTNAG | 6 cut(s) | 18, 321, 915, 1038, 1413, 1500 |
| BstF5I | GGATG | 4 cut(s) | 603, 694, 1033, 1194 |
| BstFNI | CGCG | 1 cut(s) | 1637 |
| BstH2I | RGCGCY | 1 cut(s) | 1679 |
| BstHHI | GCGC | 1 cut(s) | 1678 |
| BstKTI | GATC | 8 cut(s) | 406, 446, 638, 797, 817, 934, 964, 1383 |
| BstMAI | GTCTC | 1 cut(s) | 358 |
| BstMBI | GATC | 8 cut(s) | 403, 443, 635, 794, 814, 931, 961, 1380 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 84 |
| BstNI | CCWGG | 3 cut(s) | 771, 1199, 1680 |
| BstNSI | RCATGY | 2 cut(s) | 428, 1163 |
| BstSCI | CCNGG | 3 cut(s) | 769, 1197, 1678 |
| BstUI | CGCG | 1 cut(s) | 1637 |
| BstV2I | GAAGAC | 1 cut(s) | 1195 |
| BstX2I | RGATCY | 3 cut(s) | 635, 961, 1380 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 760 |
| BstYI | RGATCY | 3 cut(s) | 635, 961, 1380 |
| Bsu15I | ATCGAT | 2 cut(s) | 222, 237 |
| BsuRI | GGCC | 3 cut(s) | 630, 752, 1077 |
| BsuTUI | ATCGAT | 2 cut(s) | 222, 237 |
| BtsCI | GGATG | 4 cut(s) | 603, 694, 1033, 1194 |
| BtsI | GCAGTG | 1 cut(s) | 1053 |
| BtsIMutI | CAGTG | 1 cut(s) | 1053 |
| Cac8I | GCNNGC | 1 cut(s) | 1116 |
| CfoI | GCGC | 1 cut(s) | 1678 |
| Cfr13I | GGNCC | 2 cut(s) | 767, 1653 |
| ClaI | ATCGAT | 2 cut(s) | 222, 237 |
| CseI | GACGC | 2 cut(s) | 1165, 1643 |
| Csp6I | GTAC | 2 cut(s) | 919, 1046 |
| CviQI | GTAC | 2 cut(s) | 919, 1046 |
| DdeI | CTNAG | 6 cut(s) | 18, 321, 915, 1038, 1413, 1500 |
| DpnI | GATC | 8 cut(s) | 405, 445, 637, 796, 816, 933, 963, 1382 |
| DpnII | GATC | 8 cut(s) | 403, 443, 635, 794, 814, 931, 961, 1380 |
| DraI | TTTAAA | 1 cut(s) | 249 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1199 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1199 |
| Eam1104I | CTCTTC | 2 cut(s) | 1596, 1599 |
| EarI | CTCTTC | 2 cut(s) | 1596, 1599 |
| EciI | GGCGGA | 1 cut(s) | 1181 |
| Eco130I | CCWWGG | 4 cut(s) | 631, 1279, 1324, 1432 |
| Eco24I | GRGCYC | 1 cut(s) | 846 |
| Eco47I | GGWCC | 2 cut(s) | 767, 1653 |
| Eco57I | CTGAAG | 4 cut(s) | 72, 306, 645, 691 |
| EcoO109I | RGGNCCY | 1 cut(s) | 767 |
| EcoRI | GAATTC | 2 cut(s) | 941, 1401 |
| EcoRII | CCWGG | 3 cut(s) | 769, 1197, 1678 |
| EcoT14I | CCWWGG | 4 cut(s) | 631, 1279, 1324, 1432 |
| EcoT22I | ATGCAT | 1 cut(s) | 1035 |
| EcoT38I | GRGCYC | 1 cut(s) | 846 |
| ErhI | CCWWGG | 4 cut(s) | 631, 1279, 1324, 1432 |
| Esp3I | CGTCTC | 1 cut(s) | 358 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1280, 1312 |
| FblI | GTMKAC | 1 cut(s) | 1182 |
| FokI | GGATG | 4 cut(s) | 590, 701, 1020, 1181 |
| FriOI | GRGCYC | 1 cut(s) | 846 |
| FspBI | CTAG | 7 cut(s) | 360, 479, 996, 1280, 1325, 1421, 1667 |
| GlaI | GCGC | 1 cut(s) | 1677 |
| GsuI | CTGGAG | 2 cut(s) | 996, 1610 |
| HaeII | RGCGCY | 1 cut(s) | 1679 |
| HaeIII | GGCC | 3 cut(s) | 630, 752, 1077 |
| HapII | CCGG | 1 cut(s) | 1656 |
| HgaI | GACGC | 2 cut(s) | 1165, 1643 |
| HhaI | GCGC | 1 cut(s) | 1678 |
| Hin6I | GCGC | 1 cut(s) | 1676 |
| HinP1I | GCGC | 1 cut(s) | 1676 |
| HincII | GTYRAC | 1 cut(s) | 1482 |
| HindII | GTYRAC | 1 cut(s) | 1482 |
| HinfI | GANTC | 4 cut(s) | 14, 741, 1417, 1428 |
| HpaI | GTTAAC | 1 cut(s) | 1482 |
| HpaII | CCGG | 1 cut(s) | 1656 |
| HphI | GGTGA | 5 cut(s) | 538, 571, 811, 824, 1522 |
| Hpy166II | GTNNAC | 4 cut(s) | 296, 1046, 1183, 1482 |
| Hpy188I | TCNGA | 7 cut(s) | 124, 625, 640, 710, 792, 936, 947 |
| Hpy8I | GTNNAC | 4 cut(s) | 296, 1046, 1183, 1482 |
| Hpy99I | CGWCG | 1 cut(s) | 1181 |
| HpyAV | CCTTC | 4 cut(s) | 195, 620, 931, 999 |
| HpyCH4III | ACNGT | 1 cut(s) | 457 |
| HpyCH4IV | ACGT | 1 cut(s) | 1023 |
| HpyCH4V | TGCA | 7 cut(s) | 11, 461, 473, 877, 1033, 1058, 1575 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 84 |
| HpyF3I | CTNAG | 6 cut(s) | 18, 321, 915, 1038, 1413, 1500 |
| HpySE526I | ACGT | 1 cut(s) | 1023 |
| HspAI | GCGC | 1 cut(s) | 1676 |
| KspAI | GTTAAC | 1 cut(s) | 1482 |
| Kzo9I | GATC | 8 cut(s) | 403, 443, 635, 794, 814, 931, 961, 1380 |
| LmnI | GCTCC | 3 cut(s) | 537, 1015, 1123 |
| LweI | GCATC | 3 cut(s) | 1020, 1042, 1399 |
| MaeI | CTAG | 7 cut(s) | 360, 479, 996, 1280, 1325, 1421, 1667 |
| MaeII | ACGT | 1 cut(s) | 1023 |
| MaeIII | GTNAC | 1 cut(s) | 523 |
| MalI | GATC | 8 cut(s) | 405, 445, 637, 796, 816, 933, 963, 1382 |
| MboI | GATC | 8 cut(s) | 403, 443, 635, 794, 814, 931, 961, 1380 |
| MfeI | CAATTG | 1 cut(s) | 89 |
| MflI | RGATCY | 3 cut(s) | 635, 961, 1380 |
| MhlI | GDGCHC | 1 cut(s) | 846 |
| MlyI | GAGTC | 3 cut(s) | 23, 735, 1426 |
| MmeI | TCCRAC | 2 cut(s) | 694, 1384 |
| Mph1103I | ATGCAT | 1 cut(s) | 1035 |
| MroXI | GAANNNNTTC | 3 cut(s) | 279, 941, 1295 |
| MseI | TTAA | 5 cut(s) | 248, 957, 980, 1110, 1481 |
| MslI | CAYNNNNRTG | 2 cut(s) | 423, 1239 |
| MspCI | CTTAAG | 1 cut(s) | 1109 |
| MspI | CCGG | 1 cut(s) | 1656 |
| MspR9I | CCNGG | 3 cut(s) | 771, 1199, 1680 |
| MunI | CAATTG | 1 cut(s) | 89 |
| Mva1269I | GAATGC | 3 cut(s) | 44, 892, 1016 |
| MvaI | CCWGG | 3 cut(s) | 771, 1199, 1680 |
| MvnI | CGCG | 1 cut(s) | 1637 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 84 |
| NdeII | GATC | 8 cut(s) | 403, 443, 635, 794, 814, 931, 961, 1380 |
| NlaIV | GGNNCC | 2 cut(s) | 183, 539 |
| NsiI | ATGCAT | 1 cut(s) | 1035 |
| NspI | RCATGY | 2 cut(s) | 428, 1163 |
| PctI | GAATGC | 3 cut(s) | 44, 892, 1016 |
| PdmI | GAANNNNTTC | 3 cut(s) | 279, 941, 1295 |
| PfeI | GAWTC | 1 cut(s) | 1428 |
| PleI | GAGTC | 3 cut(s) | 22, 735, 1425 |
| PpsI | GAGTC | 3 cut(s) | 22, 735, 1425 |
| PpuMI | RGGWCCY | 1 cut(s) | 767 |
| PshBI | ATTAAT | 1 cut(s) | 980 |
| Psp5II | RGGWCCY | 1 cut(s) | 767 |
| Psp6I | CCWGG | 3 cut(s) | 769, 1197, 1678 |
| PspGI | CCWGG | 3 cut(s) | 769, 1197, 1678 |
| PspN4I | GGNNCC | 2 cut(s) | 183, 539 |
| PspPI | GGNCC | 2 cut(s) | 767, 1653 |
| PspPPI | RGGWCCY | 1 cut(s) | 767 |
| PsuI | RGATCY | 3 cut(s) | 635, 961, 1380 |
| RsaI | GTAC | 2 cut(s) | 920, 1047 |
| RsaNI | GTAC | 2 cut(s) | 919, 1046 |
| RseI | CAYNNNNRTG | 2 cut(s) | 423, 1239 |
| SaqAI | TTAA | 5 cut(s) | 248, 957, 980, 1110, 1481 |
| Sau3AI | GATC | 8 cut(s) | 403, 443, 635, 794, 814, 931, 961, 1380 |
| Sau96I | GGNCC | 2 cut(s) | 767, 1653 |
| SchI | GAGTC | 3 cut(s) | 23, 735, 1426 |
| ScrFI | CCNGG | 3 cut(s) | 771, 1199, 1680 |
| SduI | GDGCHC | 1 cut(s) | 846 |
| SfaNI | GCATC | 3 cut(s) | 1020, 1042, 1399 |
| SinI | GGWCC | 2 cut(s) | 767, 1653 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 423, 1239 |
| SmlI | CTYRAG | 2 cut(s) | 1109, 1373 |
| SmoI | CTYRAG | 2 cut(s) | 1109, 1373 |
| SpeI | ACTAGT | 1 cut(s) | 359 |
| SsiI | CCGC | 3 cut(s) | 1166, 1364, 1533 |
| SspI | AATATT | 2 cut(s) | 116, 613 |
| SspMI | CTAG | 7 cut(s) | 360, 479, 996, 1280, 1325, 1421, 1667 |
| StyD4I | CCNGG | 3 cut(s) | 769, 1197, 1678 |
| StyI | CCWWGG | 4 cut(s) | 631, 1279, 1324, 1432 |
| TaaI | ACNGT | 1 cut(s) | 457 |
| TaiI | ACGT | 1 cut(s) | 1026 |
| TaqI | TCGA | 6 cut(s) | 203, 222, 237, 344, 840, 1308 |
| TaqII | GACCGA | 1 cut(s) | 753 |
| TatI | WGTACW | 1 cut(s) | 918 |
| TfiI | GAWTC | 1 cut(s) | 1428 |
| Tru1I | TTAA | 5 cut(s) | 248, 957, 980, 1110, 1481 |
| Tru9I | TTAA | 5 cut(s) | 248, 957, 980, 1110, 1481 |
| TscAI | CASTG | 1 cut(s) | 1060 |
| TspRI | CASTG | 1 cut(s) | 1060 |
| Vha464I | CTTAAG | 1 cut(s) | 1109 |
| VpaK11BI | GGWCC | 2 cut(s) | 767, 1653 |
| VspI | ATTAAT | 1 cut(s) | 980 |
| XapI | RAATTY | 5 cut(s) | 250, 395, 836, 941, 1401 |
| XbaI | TCTAGA | 2 cut(s) | 478, 1420 |
| XceI | RCATGY | 2 cut(s) | 428, 1163 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 853 |
| XmaJI | CCTAGG | 2 cut(s) | 1279, 1324 |
| XmiI | GTMKAC | 1 cut(s) | 1182 |
| XmnI | GAANNNNTTC | 3 cut(s) | 279, 941, 1295 |
| XspI | CTAG | 7 cut(s) | 360, 479, 996, 1280, 1325, 1421, 1667 |
| Zsp2I | ATGCAT | 1 cut(s) | 1035 |