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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 179 |
| AcuI | CTGAAG | 2 cut(s) | 34, 110 |
| AgsI | TTSAA | 1 cut(s) | 185 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 177, 209 |
| AluBI | AGCT | 2 cut(s) | 275, 316 |
| AluI | AGCT | 2 cut(s) | 275, 316 |
| Alw21I | GWGCWC | 1 cut(s) | 195 |
| Alw26I | GTCTC | 3 cut(s) | 54, 245, 327 |
| ApoI | RAATTY | 1 cut(s) | 179 |
| AspS9I | GGNCC | 1 cut(s) | 110 |
| AsuHPI | GGTGA | 1 cut(s) | 167 |
| AsuII | TTCGAA | 1 cut(s) | 240 |
| AvaII | GGWCC | 1 cut(s) | 110 |
| BbsI | GAAGAC | 2 cut(s) | 40, 84 |
| Bbv12I | GWGCWC | 1 cut(s) | 195 |
| BclI | TGATCA | 1 cut(s) | 157 |
| BcoDI | GTCTC | 3 cut(s) | 54, 245, 327 |
| BfaI | CTAG | 4 cut(s) | 258, 276, 336, 362 |
| Bme18I | GGWCC | 1 cut(s) | 110 |
| BmgT120I | GGNCC | 1 cut(s) | 110 |
| BmsI | GCATC | 1 cut(s) | 301 |
| BpiI | GAAGAC | 2 cut(s) | 40, 84 |
| Bpu14I | TTCGAA | 1 cut(s) | 240 |
| BpuEI | CTTGAG | 3 cut(s) | 75, 281, 338 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 51, 81, 177, 207 |
| BseGI | GGATG | 1 cut(s) | 282 |
| BsiHKAI | GWGCWC | 1 cut(s) | 195 |
| BsmAI | GTCTC | 3 cut(s) | 54, 245, 327 |
| Bsp119I | TTCGAA | 1 cut(s) | 240 |
| Bsp1286I | GDGCHC | 1 cut(s) | 195 |
| Bsp143I | GATC | 2 cut(s) | 137, 157 |
| BspT104I | TTCGAA | 1 cut(s) | 240 |
| BssMI | GATC | 2 cut(s) | 137, 157 |
| Bst6I | CTCTTC | 2 cut(s) | 129, 243 |
| BstBI | TTCGAA | 1 cut(s) | 240 |
| BstF5I | GGATG | 1 cut(s) | 282 |
| BstKTI | GATC | 2 cut(s) | 140, 160 |
| BstMAI | GTCTC | 3 cut(s) | 54, 245, 327 |
| BstMBI | GATC | 2 cut(s) | 137, 157 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 298 |
| BstV2I | GAAGAC | 2 cut(s) | 40, 84 |
| BtsCI | GGATG | 1 cut(s) | 282 |
| Cfr13I | GGNCC | 1 cut(s) | 110 |
| CviAII | CATG | 1 cut(s) | 287 |
| CviJI | RGCY | 3 cut(s) | 175, 275, 316 |
| CviKI_1 | RGCY | 3 cut(s) | 175, 275, 316 |
| DpnI | GATC | 2 cut(s) | 139, 159 |
| DpnII | GATC | 2 cut(s) | 137, 157 |
| Eam1104I | CTCTTC | 2 cut(s) | 129, 243 |
| EarI | CTCTTC | 2 cut(s) | 129, 243 |
| Eco47I | GGWCC | 1 cut(s) | 110 |
| Eco57I | CTGAAG | 2 cut(s) | 34, 110 |
| FaeI | CATG | 1 cut(s) | 290 |
| FaiI | YATR | 3 cut(s) | 162, 288, 327 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 69, 101, 204, 236 |
| FatI | CATG | 1 cut(s) | 286 |
| FbaI | TGATCA | 1 cut(s) | 157 |
| FokI | GGATG | 1 cut(s) | 269 |
| FspBI | CTAG | 4 cut(s) | 258, 276, 336, 362 |
| Hin1II | CATG | 1 cut(s) | 290 |
| HindIII | AAGCTT | 1 cut(s) | 314 |
| HinfI | GANTC | 1 cut(s) | 320 |
| HphI | GGTGA | 1 cut(s) | 167 |
| Hpy188I | TCNGA | 4 cut(s) | 31, 53, 135, 309 |
| Hpy188III | TCNNGA | 2 cut(s) | 341, 352 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 298 |
| Hsp92II | CATG | 1 cut(s) | 290 |
| Ksp22I | TGATCA | 1 cut(s) | 157 |
| Kzo9I | GATC | 2 cut(s) | 137, 157 |
| LmnI | GCTCC | 1 cut(s) | 198 |
| LpnPI | CCDG | 2 cut(s) | 58, 201 |
| LweI | GCATC | 1 cut(s) | 301 |
| MaeI | CTAG | 4 cut(s) | 258, 276, 336, 362 |
| MalI | GATC | 2 cut(s) | 139, 159 |
| MboI | GATC | 2 cut(s) | 137, 157 |
| MboII | GAAGA | 9 cut(s) | 40, 47, 89, 116, 254, 257, 260, 275, 281 |
| MhlI | GDGCHC | 1 cut(s) | 195 |
| MluCI | AATT | 1 cut(s) | 179 |
| MlyI | GAGTC | 1 cut(s) | 329 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 298 |
| NdeII | GATC | 2 cut(s) | 137, 157 |
| NlaIII | CATG | 1 cut(s) | 290 |
| NspV | TTCGAA | 1 cut(s) | 240 |
| PleI | GAGTC | 1 cut(s) | 328 |
| PpsI | GAGTC | 1 cut(s) | 328 |
| PspPI | GGNCC | 1 cut(s) | 110 |
| Sau3AI | GATC | 2 cut(s) | 137, 157 |
| Sau96I | GGNCC | 1 cut(s) | 110 |
| SchI | GAGTC | 1 cut(s) | 329 |
| SduI | GDGCHC | 1 cut(s) | 195 |
| SetI | ASST | 4 cut(s) | 112, 190, 277, 318 |
| SfaNI | GCATC | 1 cut(s) | 301 |
| SfuI | TTCGAA | 1 cut(s) | 240 |
| SinI | GGWCC | 1 cut(s) | 110 |
| SmlI | CTYRAG | 3 cut(s) | 90, 296, 317 |
| SmoI | CTYRAG | 3 cut(s) | 90, 296, 317 |
| Sse9I | AATT | 1 cut(s) | 179 |
| SspMI | CTAG | 4 cut(s) | 258, 276, 336, 362 |
| TaqI | TCGA | 2 cut(s) | 228, 240 |
| TasI | AATT | 1 cut(s) | 179 |
| TspDTI | ATGAA | 3 cut(s) | 48, 271, 282 |
| VpaK11BI | GGWCC | 1 cut(s) | 110 |
| XapI | RAATTY | 1 cut(s) | 179 |
| XspI | CTAG | 4 cut(s) | 258, 276, 336, 362 |