FvH4_2g32021

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001617
Physical Location & Seq
Reverse (-)
24393379 .. 24393873
495 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g32021.t1

Sequence Viewer

Length: 495 bp
ATGGATTCCCCCAGTAACTACTTCAAGACAAACTTTGACGGCTCAGTAATCCCTACTTCATCTGCTGCTGGGTTCGTTATTAGAGATTCTGTTGGCTCTCCTATTATGTCGGGTGCGCGGAGGCTTCACAACTCTTCCGTTCCCCTGGCTGAATACATGACTTTAAAAGAATGGTTTCTTGTCGCTAAAAGACGCAACTGCAACCAAATCAAAGTTGAAGGAGATTCTTATCATCAATTGTGTCAAAATGTCTTGTGCTATTGGAAGCTCAAGTCCTTGATCCGAGATGTCTTAGCTCTTACCGATTCTTTTGTCAGCATCACCTTCTCCCACACGTACAGAGAGGCGAATTTTCTTGCGGACGCTCTTGCTTCGGTTGGTCATTCTCTTTTCTCACCGATGTGTTGGTTTGATGGGATCCCTCCCCAAGCTCAGATGGCTCTTCTTATGGATTCTTCTTGTTTTCAAACCTGCTCCCATTTCGAGTCAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

165

Amino Acids

18.41

Weight (kDa)

6.94

Isoelectric Point (pI)

43.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 11 - 125 3.6e-15 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 479
AccII CGCG 1 cut(s) 118
AciI CCGC 2 cut(s) 118, 359
AclWI GGATC 3 cut(s) 274, 412, 425
AcsI RAATTY 1 cut(s) 349
AfaI GTAC 1 cut(s) 338
AflIII ACRYGT 1 cut(s) 333
AgsI TTSAA 3 cut(s) 25, 218, 467
AjnI CCWGG 1 cut(s) 144
AleI CACNNNNGTG 1 cut(s) 400
AluBI AGCT 3 cut(s) 268, 296, 431
AluI AGCT 3 cut(s) 268, 296, 431
AlwI GGATC 3 cut(s) 274, 412, 425
ApeKI GCWGC 1 cut(s) 65
ApoI RAATTY 1 cut(s) 349
Asp700I GAANNNNTTC 1 cut(s) 174
AspLEI GCGC 1 cut(s) 118
AsuHPI GGTGA 2 cut(s) 313, 387
BamHI GGATCC 1 cut(s) 417
BbvI GCAGC 1 cut(s) 52
BccI CCATC 2 cut(s) 407, 430
BceAI ACGGC 1 cut(s) 55
BciT130I CCWGG 1 cut(s) 146
BfuAI ACCTGC 1 cut(s) 479
BisI GCNGC 1 cut(s) 66
BlsI GCNGC 1 cut(s) 67
Bme1390I CCNGG 1 cut(s) 146
BmiI GGNNCC 1 cut(s) 419
BmrFI CCNGG 1 cut(s) 146
BmrI ACTGGG 1 cut(s) 6
BmsI GCATC 1 cut(s) 327
BmuI ACTGGG 1 cut(s) 6
BpuEI CTTGAG 1 cut(s) 254
BsaAI YACGTR 1 cut(s) 336
BsaBI GATNNNNATC 1 cut(s) 228
BsaJI CCNNGG 1 cut(s) 144
Bse1I ACTGG 1 cut(s) 12
Bse8I GATNNNNATC 1 cut(s) 228
BseBI CCWGG 1 cut(s) 146
BseDI CCNNGG 1 cut(s) 144
BseJI GATNNNNATC 1 cut(s) 228
BseMII CTCAG 2 cut(s) 57, 446
BseNI ACTGG 1 cut(s) 12
BseXI GCAGC 1 cut(s) 52
BseYI CCCAGC 1 cut(s) 68
Bsh1236I CGCG 1 cut(s) 118
Bsp143I GATC 2 cut(s) 279, 417
BspACI CCGC 2 cut(s) 118, 359
BspCNI CTCAG 2 cut(s) 56, 445
BspFNI CGCG 1 cut(s) 118
BspLI GGNNCC 1 cut(s) 419
BspMI ACCTGC 1 cut(s) 479
BspPI GGATC 3 cut(s) 274, 412, 425
BspQI GCTCTTC 1 cut(s) 447
BsrI ACTGG 1 cut(s) 12
BssECI CCNNGG 1 cut(s) 144
BssMI GATC 2 cut(s) 279, 417
Bst2UI CCWGG 1 cut(s) 146
Bst6I CTCTTC 2 cut(s) 139, 447
BstBAI YACGTR 1 cut(s) 336
BstDEI CTNAG 3 cut(s) 43, 292, 432
BstFNI CGCG 1 cut(s) 118
BstHHI GCGC 1 cut(s) 118
BstKTI GATC 2 cut(s) 282, 420
BstMBI GATC 2 cut(s) 279, 417
BstMWI GCNNNNNNNGC 1 cut(s) 437
BstNI CCWGG 1 cut(s) 146
BstSCI CCNGG 1 cut(s) 144
BstUI CGCG 1 cut(s) 118
BstV1I GCAGC 1 cut(s) 52
BstX2I RGATCY 1 cut(s) 417
BstYI RGATCY 1 cut(s) 417
BveI ACCTGC 1 cut(s) 479
CfoI GCGC 1 cut(s) 118
CseI GACGC 2 cut(s) 201, 371
Csp6I GTAC 1 cut(s) 337
CviAII CATG 1 cut(s) 157
CviJI RGCY 8 cut(s) 42, 96, 124, 149, 268, 296, 431, 440
CviKI_1 RGCY 8 cut(s) 42, 96, 124, 149, 268, 296, 431, 440
CviQI GTAC 1 cut(s) 337
DdeI CTNAG 3 cut(s) 43, 292, 432
DpnI GATC 2 cut(s) 281, 419
DpnII GATC 2 cut(s) 279, 417
DraI TTTAAA 1 cut(s) 165
Eam1104I CTCTTC 2 cut(s) 139, 447
EarI CTCTTC 2 cut(s) 139, 447
EcoRII CCWGG 1 cut(s) 144
FaeI CATG 1 cut(s) 160
FaiI YATR 3 cut(s) 107, 158, 449
FalI AAGNNNNNCTT 2 cut(s) 17, 49
FatI CATG 1 cut(s) 156
Fnu4HI GCNGC 1 cut(s) 66
Fsp4HI GCNGC 1 cut(s) 66
GlaI GCGC 1 cut(s) 117
GluI GCNGC 1 cut(s) 66
GsaI CCCAGC 1 cut(s) 72
HgaI GACGC 2 cut(s) 201, 371
HhaI GCGC 1 cut(s) 118
Hin1II CATG 1 cut(s) 160
Hin6I GCGC 1 cut(s) 116
HinP1I GCGC 1 cut(s) 116
HinfI GANTC 6 cut(s) 5, 86, 224, 305, 452, 485
HphI GGTGA 2 cut(s) 313, 387
Hpy188I TCNGA 2 cut(s) 284, 435
Hpy188III TCNNGA 2 cut(s) 25, 489
HpyAV CCTTC 2 cut(s) 212, 334
HpyCH4IV ACGT 1 cut(s) 335
HpyCH4V TGCA 1 cut(s) 201
HpyF10VI GCNNNNNNNGC 1 cut(s) 437
HpyF3I CTNAG 3 cut(s) 43, 292, 432
HpySE526I ACGT 1 cut(s) 335
Hsp92II CATG 1 cut(s) 160
HspAI GCGC 1 cut(s) 116
Kzo9I GATC 2 cut(s) 279, 417
LguI GCTCTTC 1 cut(s) 447
LmnI GCTCC 1 cut(s) 479
LpnPI CCDG 5 cut(s) 25, 54, 131, 158, 484
Lsp1109I GCAGC 1 cut(s) 52
LweI GCATC 1 cut(s) 327
MaeII ACGT 1 cut(s) 335
MaeIII GTNAC 1 cut(s) 14
MalI GATC 2 cut(s) 281, 419
MboI GATC 2 cut(s) 279, 417
MboII GAAGA 3 cut(s) 126, 434, 447
MfeI CAATTG 1 cut(s) 236
MflI RGATCY 1 cut(s) 417
MluCI AATT 2 cut(s) 236, 349
MlyI GAGTC 1 cut(s) 494
MnlI CCTC 3 cut(s) 114, 337, 432
MroXI GAANNNNTTC 1 cut(s) 174
MseI TTAA 1 cut(s) 164
MslI CAYNNNNRTG 1 cut(s) 400
MspR9I CCNGG 1 cut(s) 146
MunI CAATTG 1 cut(s) 236
MvaI CCWGG 1 cut(s) 146
MvnI CGCG 1 cut(s) 118
MwoI GCNNNNNNNGC 1 cut(s) 437
NdeII GATC 2 cut(s) 279, 417
NlaIII CATG 1 cut(s) 160
NlaIV GGNNCC 1 cut(s) 419
OliI CACNNNNGTG 1 cut(s) 400
PciSI GCTCTTC 1 cut(s) 447
PdmI GAANNNNTTC 1 cut(s) 174
PfeI GAWTC 5 cut(s) 5, 86, 224, 305, 452
PkrI GCNGC 1 cut(s) 67
PleI GAGTC 1 cut(s) 493
PpsI GAGTC 1 cut(s) 493
Ppu21I YACGTR 1 cut(s) 336
Psp6I CCWGG 1 cut(s) 144
PspFI CCCAGC 1 cut(s) 68
PspGI CCWGG 1 cut(s) 144
PspN4I GGNNCC 1 cut(s) 419
PsuI RGATCY 1 cut(s) 417
RsaI GTAC 1 cut(s) 338
RsaNI GTAC 1 cut(s) 337
RseI CAYNNNNRTG 1 cut(s) 400
SapI GCTCTTC 1 cut(s) 447
SaqAI TTAA 1 cut(s) 164
SatI GCNGC 1 cut(s) 66
Sau3AI GATC 2 cut(s) 279, 417
SchI GAGTC 1 cut(s) 494
ScrFI CCNGG 1 cut(s) 146
SetI ASST 6 cut(s) 270, 298, 326, 338, 433, 473
SfaNI GCATC 1 cut(s) 327
SmiMI CAYNNNNRTG 1 cut(s) 400
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
Sse9I AATT 2 cut(s) 236, 349
SsiI CCGC 2 cut(s) 118, 359
StyD4I CCNGG 1 cut(s) 144
TaiI ACGT 1 cut(s) 338
TaqI TCGA 1 cut(s) 483
TasI AATT 2 cut(s) 236, 349
TfiI GAWTC 5 cut(s) 5, 86, 224, 305, 452
Tru1I TTAA 1 cut(s) 164
Tru9I TTAA 1 cut(s) 164
TseI GCWGC 1 cut(s) 65
TspDTI ATGAA 1 cut(s) 48
TspGWI ACGGA 1 cut(s) 127
XapI RAATTY 1 cut(s) 349
XmnI GAANNNNTTC 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.