RchiOBHm_Chr4g0422501

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001617
Physical Location & Seq
Forward (+)
48088893 .. 48089495
603 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39203

Sequence Viewer

Length: 603 bp
ATGATTGTCCATGCTGCAGCTGCTGCCGCATATAATTATCGGGCTCACAACTCCGTTCCTACTATGTCTCGTGCAAGGGATTATGGCCTAATCAGATGGGCCCCTCCTCAGGAAGGTTCAGTCAAGCTAAATTTTGATGGTTCGGTTGATGGCTCAAATATGGCAGCTGCCAGGTTTGTGATCAGAGATCACTCAGGCTTTCCGATTGTGGCAGGAAGTAGAAAGATTGGTTTCTCTTCTGTGCCGGTTGCAGAGTGTAGCGCCCTCAAAGACGGTCTCCTCCAGGCGCTGCATAGCAACTTATCCAAAATTTCTGTTGAAGGTGATTCAAAGCTGGTCATTGACTGCATCAAGAAGATTCATGCTCCCCCTTGGCGTTTGAGACCTTTGATTAGAGACATTCAGATTATCTCTTCCTTCTTTGAGGACATTAGTTTCTCTCATGTTCCTCGAGAGGCAAACTTCGTGGCAGATCCGGTTACTTCCCTGGGTCACCAAGCGTTTGAACCAACTATCTGGAGAAACAAACTTCCTCTCTCGGCCAGTTCTGCTTTCAGTTTAGATCAGTTAGGGTGTGGTGTTTCTAGGGGTTTTCAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

200

Amino Acids

21.77

Weight (kDa)

9.12

Isoelectric Point (pI)

35.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 44 - 159 7.7e-22 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 27
AclWI GGATC 1 cut(s) 467
AcoI YGGCCR 1 cut(s) 540
AcsI RAATTY 2 cut(s) 130, 309
AfiI CCNNNNNNNGG 2 cut(s) 109, 113
AgsI TTSAA 3 cut(s) 320, 330, 506
AjnI CCWGG 3 cut(s) 170, 282, 486
AluBI AGCT 4 cut(s) 20, 127, 167, 334
AluI AGCT 4 cut(s) 20, 127, 167, 334
Alw26I GTCTC 4 cut(s) 72, 281, 376, 390
AlwI GGATC 1 cut(s) 467
AlwNI CAGNNNCTG 2 cut(s) 23, 289
Ama87I CYCGRG 1 cut(s) 450
AoxI GGCC 3 cut(s) 85, 99, 540
ApaI GGGCCC 1 cut(s) 103
ApeKI GCWGC 7 cut(s) 14, 17, 20, 23, 164, 167, 289
ApoI RAATTY 2 cut(s) 130, 309
AspLEI GCGC 2 cut(s) 263, 289
AspS9I GGNCC 2 cut(s) 99, 100
AsuHPI GGTGA 2 cut(s) 335, 485
AvaI CYCGRG 1 cut(s) 450
AxyI CCTNAGG 1 cut(s) 108
BaeGI GKGCMC 1 cut(s) 103
BanII GRGCYC 2 cut(s) 46, 103
BauI CACGAG 1 cut(s) 69
BbvI GCAGC 6 cut(s) 7, 10, 29, 154, 176, 276
BccI CCATC 3 cut(s) 90, 131, 143
BciT130I CCWGG 3 cut(s) 172, 284, 488
BclI TGATCA 1 cut(s) 180
BcoDI GTCTC 4 cut(s) 72, 281, 376, 390
BfaI CTAG 1 cut(s) 585
BfmI CTRYAG 1 cut(s) 15
BfoI RGCGCY 2 cut(s) 264, 290
BisI GCNGC 8 cut(s) 15, 18, 21, 24, 27, 165, 168, 290
BlsI GCNGC 8 cut(s) 16, 19, 22, 25, 28, 166, 169, 291
Bme1390I CCNGG 3 cut(s) 172, 284, 488
BmeT110I CYCGRG 1 cut(s) 450
BmgT120I GGNCC 2 cut(s) 99, 100
BmiI GGNNCC 2 cut(s) 101, 102
BmrFI CCNGG 3 cut(s) 172, 284, 488
BmsI GCATC 1 cut(s) 357
BpmI CTGGAG 2 cut(s) 266, 538
BsaI GGTCTC 2 cut(s) 281, 376
BsaJI CCNNGG 3 cut(s) 371, 486, 487
BsaWI WCCGGW 1 cut(s) 475
Bsc4I CCNNNNNNNGG 2 cut(s) 109, 113
Bse118I RCCGGY 1 cut(s) 244
Bse1I ACTGG 1 cut(s) 543
Bse21I CCTNAGG 1 cut(s) 108
BseBI CCWGG 3 cut(s) 172, 284, 488
BseDI CCNNGG 3 cut(s) 371, 486, 487
BseLI CCNNNNNNNGG 2 cut(s) 109, 113
BseMII CTCAG 2 cut(s) 122, 207
BseNI ACTGG 1 cut(s) 543
BseRI GAGGAG 2 cut(s) 96, 269
BseSI GKGCMC 1 cut(s) 103
BseXI GCAGC 6 cut(s) 7, 10, 29, 154, 176, 276
BshFI GGCC 3 cut(s) 87, 101, 542
BsiHKCI CYCGRG 1 cut(s) 450
BsiSI CCGG 2 cut(s) 245, 476
BslI CCNNNNNNNGG 2 cut(s) 109, 113
BsmAI GTCTC 4 cut(s) 72, 281, 376, 390
BsnI GGCC 3 cut(s) 87, 101, 542
Bso31I GGTCTC 2 cut(s) 281, 376
BsoBI CYCGRG 1 cut(s) 450
Bsp120I GGGCCC 1 cut(s) 99
Bsp1286I GDGCHC 2 cut(s) 46, 103
Bsp143I GATC 4 cut(s) 180, 187, 472, 562
BspACI CCGC 1 cut(s) 27
BspANI GGCC 3 cut(s) 87, 101, 542
BspCNI CTCAG 2 cut(s) 121, 206
BspLI GGNNCC 2 cut(s) 101, 102
BspMAI CTGCAG 1 cut(s) 19
BspPI GGATC 1 cut(s) 467
BspTNI GGTCTC 2 cut(s) 281, 376
BsrFI RCCGGY 1 cut(s) 244
BsrI ACTGG 1 cut(s) 543
BssAI RCCGGY 1 cut(s) 244
BssECI CCNNGG 3 cut(s) 371, 486, 487
BssMI GATC 4 cut(s) 180, 187, 472, 562
BssSI CACGAG 1 cut(s) 69
BssT1I CCWWGG 1 cut(s) 371
Bst2BI CACGAG 1 cut(s) 69
Bst2UI CCWGG 3 cut(s) 172, 284, 488
Bst4CI ACNGT 1 cut(s) 275
Bst6I CTCTTC 2 cut(s) 241, 418
BstAPI GCANNNNNTGC 1 cut(s) 23
BstDEI CTNAG 2 cut(s) 108, 193
BstEII GGTNACC 1 cut(s) 491
BstENI CCTNNNNNAGG 1 cut(s) 111
BstH2I RGCGCY 2 cut(s) 264, 290
BstHHI GCGC 2 cut(s) 263, 289
BstKTI GATC 4 cut(s) 183, 190, 475, 565
BstMAI GTCTC 4 cut(s) 72, 281, 376, 390
BstMBI GATC 4 cut(s) 180, 187, 472, 562
BstMWI GCNNNNNNNGC 4 cut(s) 20, 23, 26, 548
BstNI CCWGG 3 cut(s) 172, 284, 488
BstPI GGTNACC 1 cut(s) 491
BstSCI CCNGG 3 cut(s) 170, 282, 486
BstSFI CTRYAG 1 cut(s) 15
BstSLI GKGCMC 1 cut(s) 103
BstV1I GCAGC 6 cut(s) 7, 10, 29, 154, 176, 276
BstX2I RGATCY 1 cut(s) 472
BstXI CCANNNNNNTGG 1 cut(s) 516
BstYI RGATCY 1 cut(s) 472
Bsu36I CCTNAGG 1 cut(s) 108
BsuRI GGCC 3 cut(s) 87, 101, 542
CaiI CAGNNNCTG 2 cut(s) 23, 289
CfoI GCGC 2 cut(s) 263, 289
Cfr10I RCCGGY 1 cut(s) 244
Cfr13I GGNCC 2 cut(s) 99, 100
CspCI CAANNNNNGTGG 2 cut(s) 447, 482
CviAII CATG 3 cut(s) 11, 362, 443
DdeI CTNAG 2 cut(s) 108, 193
DpnI GATC 4 cut(s) 182, 189, 474, 564
DpnII GATC 4 cut(s) 180, 187, 472, 562
EaeI YGGCCR 1 cut(s) 540
Eam1104I CTCTTC 2 cut(s) 241, 418
EarI CTCTTC 2 cut(s) 241, 418
Eco130I CCWWGG 1 cut(s) 371
Eco24I GRGCYC 2 cut(s) 46, 103
Eco31I GGTCTC 2 cut(s) 281, 376
Eco81I CCTNAGG 1 cut(s) 108
Eco88I CYCGRG 1 cut(s) 450
Eco91I GGTNACC 1 cut(s) 491
EcoNI CCTNNNNNAGG 1 cut(s) 111
EcoO109I RGGNCCY 1 cut(s) 100
EcoO65I GGTNACC 1 cut(s) 491
EcoRII CCWGG 3 cut(s) 170, 282, 486
EcoT14I CCWWGG 1 cut(s) 371
EcoT38I GRGCYC 2 cut(s) 46, 103
ErhI CCWWGG 1 cut(s) 371
FaeI CATG 3 cut(s) 14, 365, 446
FaiI YATR 9 cut(s) 12, 31, 33, 65, 84, 161, 294, 363, 444
FatI CATG 3 cut(s) 10, 361, 442
FbaI TGATCA 1 cut(s) 180
Fnu4HI GCNGC 8 cut(s) 15, 18, 21, 24, 27, 165, 168, 290
FriOI GRGCYC 2 cut(s) 46, 103
Fsp4HI GCNGC 8 cut(s) 15, 18, 21, 24, 27, 165, 168, 290
FspBI CTAG 1 cut(s) 585
GlaI GCGC 2 cut(s) 262, 288
GluI GCNGC 8 cut(s) 15, 18, 21, 24, 27, 165, 168, 290
GsuI CTGGAG 2 cut(s) 266, 538
HaeII RGCGCY 2 cut(s) 264, 290
HaeIII GGCC 3 cut(s) 87, 101, 542
HapII CCGG 2 cut(s) 245, 476
HhaI GCGC 2 cut(s) 263, 289
Hin1II CATG 3 cut(s) 14, 365, 446
Hin6I GCGC 2 cut(s) 261, 287
HinP1I GCGC 2 cut(s) 261, 287
HinfI GANTC 2 cut(s) 326, 358
HpaII CCGG 2 cut(s) 245, 476
HphI GGTGA 2 cut(s) 335, 485
Hpy188I TCNGA 4 cut(s) 95, 185, 204, 405
Hpy188III TCNNGA 4 cut(s) 110, 352, 452, 517
HpyAV CCTTC 3 cut(s) 107, 314, 427
HpyCH4III ACNGT 1 cut(s) 275
HpyCH4V TGCA 5 cut(s) 17, 74, 251, 292, 348
HpyF10VI GCNNNNNNNGC 4 cut(s) 20, 23, 26, 548
HpyF3I CTNAG 2 cut(s) 108, 193
Hsp92II CATG 3 cut(s) 14, 365, 446
HspAI GCGC 2 cut(s) 261, 287
Ksp22I TGATCA 1 cut(s) 180
Kzo9I GATC 4 cut(s) 180, 187, 472, 562
LmnI GCTCC 1 cut(s) 370
Lsp1109I GCAGC 6 cut(s) 7, 10, 29, 154, 176, 276
LweI GCATC 1 cut(s) 357
MaeI CTAG 1 cut(s) 585
MaeIII GTNAC 2 cut(s) 478, 491
MalI GATC 4 cut(s) 182, 189, 474, 564
MboI GATC 4 cut(s) 180, 187, 472, 562
MboII GAAGA 3 cut(s) 228, 367, 405
MflI RGATCY 1 cut(s) 472
MhlI GDGCHC 2 cut(s) 46, 103
MluCI AATT 3 cut(s) 34, 130, 309
MnlI CCTC 8 cut(s) 114, 117, 275, 290, 418, 448, 459, 543
MspA1I CMGCKG 2 cut(s) 20, 167
MspI CCGG 2 cut(s) 245, 476
MspR9I CCNGG 3 cut(s) 172, 284, 488
MvaI CCWGG 3 cut(s) 172, 284, 488
MwoI GCNNNNNNNGC 4 cut(s) 20, 23, 26, 548
NdeII GATC 4 cut(s) 180, 187, 472, 562
NlaIII CATG 3 cut(s) 14, 365, 446
NlaIV GGNNCC 2 cut(s) 101, 102
NmeAIII GCCGAG 1 cut(s) 518
NmuCI GTSAC 1 cut(s) 491
PaeR7I CTCGAG 1 cut(s) 450
PasI CCCWGGG 1 cut(s) 487
PfeI GAWTC 2 cut(s) 326, 358
PkrI GCNGC 8 cut(s) 16, 19, 22, 25, 28, 166, 169, 291
Psp6I CCWGG 3 cut(s) 170, 282, 486
PspEI GGTNACC 1 cut(s) 491
PspGI CCWGG 3 cut(s) 170, 282, 486
PspN4I GGNNCC 2 cut(s) 101, 102
PspOMI GGGCCC 1 cut(s) 99
PspPI GGNCC 2 cut(s) 99, 100
PstI CTGCAG 1 cut(s) 19
PstNI CAGNNNCTG 2 cut(s) 23, 289
PsuI RGATCY 1 cut(s) 472
PvuII CAGCTG 2 cut(s) 20, 167
SatI GCNGC 8 cut(s) 15, 18, 21, 24, 27, 165, 168, 290
Sau3AI GATC 4 cut(s) 180, 187, 472, 562
Sau96I GGNCC 2 cut(s) 99, 100
ScrFI CCNGG 3 cut(s) 172, 284, 488
SduI GDGCHC 2 cut(s) 46, 103
SetI ASST 8 cut(s) 22, 118, 129, 169, 176, 325, 336, 388
SfaNI GCATC 1 cut(s) 357
SfcI CTRYAG 1 cut(s) 15
Sfr274I CTCGAG 1 cut(s) 450
SlaI CTCGAG 1 cut(s) 450
SmlI CTYRAG 1 cut(s) 450
SmoI CTYRAG 1 cut(s) 450
Sse9I AATT 3 cut(s) 34, 130, 309
SsiI CCGC 1 cut(s) 27
SspMI CTAG 1 cut(s) 585
StyD4I CCNGG 3 cut(s) 170, 282, 486
StyI CCWWGG 1 cut(s) 371
TaaI ACNGT 1 cut(s) 275
TaqI TCGA 1 cut(s) 451
TasI AATT 3 cut(s) 34, 130, 309
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 2 cut(s) 326, 358
TseFI GTSAC 1 cut(s) 491
TseI GCWGC 7 cut(s) 14, 17, 20, 23, 164, 167, 289
Tsp45I GTSAC 1 cut(s) 491
TspDTI ATGAA 1 cut(s) 350
TspGWI ACGGA 1 cut(s) 43
XagI CCTNNNNNAGG 1 cut(s) 111
XapI RAATTY 2 cut(s) 130, 309
XhoI CTCGAG 1 cut(s) 450
XspI CTAG 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.