Rorug06G0302200

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001617
Physical Location & Seq
Forward (+)
45359473 .. 45363732
4260 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0302200.1

Sequence Viewer

Length: 858 bp
ATGGGTCTGAGGGACATTGGAGCCACACTGCCTCCTGGGTTTCGATTCTATCCAAGTGATGAGGAGTTGGTCTGCCACTATCTCTACAAAAAGGTCACAAATGAGGAAGCTTTGAAGGGTACTCTGGTGGAAATTGACTTGCATACTTGTGAGCCATGGCAGCTTCCTGAGGTGGCAAAGCTGAACGCGAACGAGTGGTACTTCTTCAGCTTTCGTGACCGGAAGTACGCGACCGGGTTCAGAACCAACCGGGCGACGACGACCGGGTACTGGAAAGCTACCGGAAAAGACCGGATGGTGGTGGATCCGGGAACAGGGGACATCGTAGGGATGAGGAAGACGCTGGTGTTCTATCGGAACAGAGCTCCCAATGGAATTAAAACTGGGTGGATCATGCATGAGTTTCGCCTCGAGACCCCACACATGCCTCCCAAGGAGGACTGGGTTCTGTGCCGGGTTTTCCACAAAGGCAAAGGAGAGATGAACGACAACATGAACCTCAGCCCTCATGACCTTATGTTGGAGACCAAAACATCATGCACTGCTGCGTTAACATCTCCTCCAACTCATCATGACCAAAACATGCACTGTGGATATAGTAACCAGATCACCTCATTTTCCACCACACCAGTACCTCACCAAAGCCATAGCCATAGCCACAATAACCAAAGCAATTCTCTGCTCAACCTCCTTCAGCTTTCTGAAGAAAAATATACCGACTCTATCACCGAAATAAGCGCAAAAAATGACGATGATTATGGATTTTTATGGGACATGAATCTGGAAGAAAATAGCTTTGAAACAGGTACTTTGGACGACATGAGATTTGAGATGGAGAACAGCATGGTTTTGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

285

Amino Acids

32.8

Weight (kDa)

5.6

Isoelectric Point (pI)

37.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 11 - 137 1.9e-43 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 188, 230
AclWI GGATC 3 cut(s) 299, 312, 398
AcuI CTGAAG 3 cut(s) 190, 677, 723
AfaI GTAC 6 cut(s) 121, 200, 227, 269, 633, 808
AfiI CCNNNNNNNGG 4 cut(s) 270, 298, 314, 520
AgsI TTSAA 2 cut(s) 115, 800
AjnI CCWGG 1 cut(s) 34
AluBI AGCT 8 cut(s) 110, 163, 181, 210, 278, 365, 697, 795
AluI AGCT 8 cut(s) 110, 163, 181, 210, 278, 365, 697, 795
Alw21I GWGCWC 1 cut(s) 367
Alw26I GTCTC 2 cut(s) 407, 518
AlwI GGATC 3 cut(s) 299, 312, 398
Ama87I CYCGRG 1 cut(s) 410
ApeKI GCWGC 2 cut(s) 160, 545
ArsI GACNNNNNNTTYG 2 cut(s) 809, 841
AspLEI GCGC 1 cut(s) 740
AsuC2I CCSGG 5 cut(s) 235, 251, 265, 309, 455
AsuHPI GGTGA 3 cut(s) 601, 629, 718
AvaI CYCGRG 1 cut(s) 410
AxyI CCTNAGG 1 cut(s) 168
BaeI ACNNNNGTAYC 4 cut(s) 111, 144, 615, 648
BamHI GGATCC 1 cut(s) 304
BanII GRGCYC 1 cut(s) 367
BbsI GAAGAC 1 cut(s) 344
Bbv12I GWGCWC 1 cut(s) 367
BbvCI CCTCAGC 1 cut(s) 500
BbvI GCAGC 2 cut(s) 172, 532
BccI CCATC 2 cut(s) 289, 826
BcgI CGANNNNNNTGC 2 cut(s) 386, 420
BciT130I CCWGG 1 cut(s) 36
BcnI CCSGG 5 cut(s) 235, 251, 265, 309, 455
BcoDI GTCTC 2 cut(s) 407, 518
BisI GCNGC 2 cut(s) 161, 546
BlsI GCNGC 2 cut(s) 162, 547
Bme1390I CCNGG 6 cut(s) 36, 235, 251, 265, 309, 455
BmeT110I CYCGRG 1 cut(s) 410
BmiI GGNNCC 2 cut(s) 22, 306
BmrFI CCNGG 6 cut(s) 36, 235, 251, 265, 309, 455
BmrI ACTGGG 2 cut(s) 393, 451
BmuI ACTGGG 2 cut(s) 393, 451
BpiI GAAGAC 1 cut(s) 344
Bpu10I CCTNAGC 1 cut(s) 500
BpuMI CCSGG 5 cut(s) 235, 251, 265, 309, 455
BsaI GGTCTC 2 cut(s) 407, 518
BsaJI CCNNGG 3 cut(s) 35, 155, 432
BsaWI WCCGGW 3 cut(s) 219, 281, 291
BsaXI ACNNNNNCTCC 4 cut(s) 16, 46, 544, 574
Bsc4I CCNNNNNNNGG 4 cut(s) 270, 298, 314, 520
Bse1I ACTGG 4 cut(s) 275, 388, 446, 629
Bse21I CCTNAGG 1 cut(s) 168
BseBI CCWGG 1 cut(s) 36
BseDI CCNNGG 3 cut(s) 35, 155, 432
BseGI GGATG 2 cut(s) 300, 336
BseLI CCNNNNNNNGG 4 cut(s) 270, 298, 314, 520
BseMII CTCAG 2 cut(s) 159, 514
BseNI ACTGG 4 cut(s) 275, 388, 446, 629
BseRI GAGGAG 2 cut(s) 77, 549
BseXI GCAGC 2 cut(s) 172, 532
Bsh1236I CGCG 2 cut(s) 188, 230
Bsh1285I CGRYCG 2 cut(s) 234, 264
BsiEI CGRYCG 2 cut(s) 234, 264
BsiHKAI GWGCWC 1 cut(s) 367
BsiHKCI CYCGRG 1 cut(s) 410
BsiSI CCGG 8 cut(s) 220, 234, 250, 264, 282, 292, 308, 454
BslFI GGGAC 3 cut(s) 26, 332, 785
BslI CCNNNNNNNGG 4 cut(s) 270, 298, 314, 520
BsmAI GTCTC 2 cut(s) 407, 518
BsmFI GGGAC 3 cut(s) 26, 332, 785
Bso31I GGTCTC 2 cut(s) 407, 518
BsoBI CYCGRG 1 cut(s) 410
Bsp1286I GDGCHC 1 cut(s) 367
Bsp143I GATC 3 cut(s) 304, 390, 606
Bsp19I CCATGG 1 cut(s) 155
BspCNI CTCAG 2 cut(s) 160, 513
BspFNI CGCG 2 cut(s) 188, 230
BspHI TCATGA 2 cut(s) 508, 571
BspLI GGNNCC 2 cut(s) 22, 306
BspPI GGATC 3 cut(s) 299, 312, 398
BspTNI GGTCTC 2 cut(s) 407, 518
BsrI ACTGG 4 cut(s) 275, 388, 446, 629
BssECI CCNNGG 3 cut(s) 35, 155, 432
BssMI GATC 3 cut(s) 304, 390, 606
BssT1I CCWWGG 2 cut(s) 155, 432
Bst2UI CCWGG 1 cut(s) 36
Bst4CI ACNGT 1 cut(s) 590
BstDEI CTNAG 3 cut(s) 8, 168, 500
BstDSI CCRYGG 1 cut(s) 155
BstF5I GGATG 2 cut(s) 300, 336
BstFNI CGCG 2 cut(s) 188, 230
BstHHI GCGC 1 cut(s) 740
BstKTI GATC 3 cut(s) 307, 393, 609
BstMAI GTCTC 2 cut(s) 407, 518
BstMBI GATC 3 cut(s) 304, 390, 606
BstMCI CGRYCG 2 cut(s) 234, 264
BstMWI GCNNNNNNNGC 1 cut(s) 160
BstNI CCWGG 1 cut(s) 36
BstNSI RCATGY 2 cut(s) 427, 586
BstSCI CCNGG 6 cut(s) 34, 233, 249, 263, 307, 453
BstUI CGCG 2 cut(s) 188, 230
BstV1I GCAGC 2 cut(s) 172, 532
BstV2I GAAGAC 1 cut(s) 344
BstX2I RGATCY 1 cut(s) 304
BstYI RGATCY 1 cut(s) 304
Bsu36I CCTNAGG 1 cut(s) 168
BtgI CCRYGG 1 cut(s) 155
BtsCI GGATG 2 cut(s) 300, 336
BtsI GCAGTG 2 cut(s) 26, 540
BtsIMutI CAGTG 3 cut(s) 26, 540, 586
CciI TCATGA 2 cut(s) 508, 571
CfoI GCGC 1 cut(s) 740
CseI GACGC 1 cut(s) 349
Csp6I GTAC 6 cut(s) 120, 199, 226, 268, 632, 807
CviQI GTAC 6 cut(s) 120, 199, 226, 268, 632, 807
DdeI CTNAG 3 cut(s) 8, 168, 500
DpnI GATC 3 cut(s) 306, 392, 608
DpnII GATC 3 cut(s) 304, 390, 606
Ecl136II GAGCTC 1 cut(s) 365
Eco130I CCWWGG 2 cut(s) 155, 432
Eco24I GRGCYC 1 cut(s) 367
Eco31I GGTCTC 2 cut(s) 407, 518
Eco53kI GAGCTC 1 cut(s) 365
Eco57I CTGAAG 3 cut(s) 190, 677, 723
Eco81I CCTNAGG 1 cut(s) 168
Eco88I CYCGRG 1 cut(s) 410
EcoICRI GAGCTC 1 cut(s) 365
EcoRII CCWGG 1 cut(s) 34
EcoT14I CCWWGG 2 cut(s) 155, 432
EcoT22I ATGCAT 1 cut(s) 399
EcoT38I GRGCYC 1 cut(s) 367
ErhI CCWWGG 2 cut(s) 155, 432
FaqI GGGAC 3 cut(s) 26, 332, 785
Fnu4HI GCNGC 2 cut(s) 161, 546
FokI GGATG 2 cut(s) 307, 343
FriOI GRGCYC 1 cut(s) 367
Fsp4HI GCNGC 2 cut(s) 161, 546
GlaI GCGC 1 cut(s) 739
GluI GCNGC 2 cut(s) 161, 546
HapII CCGG 8 cut(s) 220, 234, 250, 264, 282, 292, 308, 454
HgaI GACGC 1 cut(s) 349
HhaI GCGC 1 cut(s) 740
Hin6I GCGC 1 cut(s) 738
HinP1I GCGC 1 cut(s) 738
HincII GTYRAC 1 cut(s) 552
HindII GTYRAC 1 cut(s) 552
HindIII AAGCTT 1 cut(s) 108
HinfI GANTC 3 cut(s) 45, 719, 778
HpaI GTTAAC 1 cut(s) 552
HpaII CCGG 8 cut(s) 220, 234, 250, 264, 282, 292, 308, 454
HphI GGTGA 3 cut(s) 601, 629, 718
Hpy166II GTNNAC 1 cut(s) 552
Hpy188I TCNGA 4 cut(s) 9, 242, 357, 703
Hpy188III TCNNGA 6 cut(s) 167, 215, 412, 509, 572, 782
Hpy8I GTNNAC 1 cut(s) 552
Hpy99I CGWCG 2 cut(s) 259, 262
HpyAV CCTTC 2 cut(s) 109, 701
HpyCH4III ACNGT 1 cut(s) 590
HpyCH4V TGCA 4 cut(s) 142, 397, 540, 586
HpyF10VI GCNNNNNNNGC 1 cut(s) 160
HpyF3I CTNAG 3 cut(s) 8, 168, 500
HspAI GCGC 1 cut(s) 738
KspAI GTTAAC 1 cut(s) 552
Kzo9I GATC 3 cut(s) 304, 390, 606
LmnI GCTCC 2 cut(s) 20, 370
Lsp1109I GCAGC 2 cut(s) 172, 532
MaeIII GTNAC 3 cut(s) 94, 215, 599
MalI GATC 3 cut(s) 306, 392, 608
MboI GATC 3 cut(s) 304, 390, 606
MboII GAAGA 4 cut(s) 196, 349, 716, 797
MflI RGATCY 1 cut(s) 304
MhlI GDGCHC 1 cut(s) 367
MluCI AATT 3 cut(s) 132, 375, 673
MlyI GAGTC 1 cut(s) 713
MmeI TCCRAC 2 cut(s) 501, 587
Mph1103I ATGCAT 1 cut(s) 399
MseI TTAA 2 cut(s) 378, 551
MslI CAYNNNNRTG 1 cut(s) 147
MspI CCGG 8 cut(s) 220, 234, 250, 264, 282, 292, 308, 454
MspR9I CCNGG 6 cut(s) 36, 235, 251, 265, 309, 455
MvaI CCWGG 1 cut(s) 36
MvnI CGCG 2 cut(s) 188, 230
MwoI GCNNNNNNNGC 1 cut(s) 160
NciI CCSGG 5 cut(s) 235, 251, 265, 309, 455
NcoI CCATGG 1 cut(s) 155
NdeII GATC 3 cut(s) 304, 390, 606
NlaIV GGNNCC 2 cut(s) 22, 306
NmuCI GTSAC 2 cut(s) 94, 215
NsiI ATGCAT 1 cut(s) 399
NspI RCATGY 2 cut(s) 427, 586
PaeR7I CTCGAG 1 cut(s) 410
PagI TCATGA 2 cut(s) 508, 571
PfeI GAWTC 2 cut(s) 45, 778
PfoI TCCNGGA 1 cut(s) 307
PkrI GCNGC 2 cut(s) 162, 547
PleI GAGTC 1 cut(s) 713
PpsI GAGTC 1 cut(s) 713
Psp124BI GAGCTC 1 cut(s) 367
Psp6I CCWGG 1 cut(s) 34
PspGI CCWGG 1 cut(s) 34
PspN4I GGNNCC 2 cut(s) 22, 306
PsrI GAACNNNNNNTAC 2 cut(s) 182, 214
PsuI RGATCY 1 cut(s) 304
RsaI GTAC 6 cut(s) 121, 200, 227, 269, 633, 808
RsaNI GTAC 6 cut(s) 120, 199, 226, 268, 632, 807
RseI CAYNNNNRTG 1 cut(s) 147
SacI GAGCTC 1 cut(s) 367
SaqAI TTAA 2 cut(s) 378, 551
SatI GCNGC 2 cut(s) 161, 546
Sau3AI GATC 3 cut(s) 304, 390, 606
SchI GAGTC 1 cut(s) 713
ScrFI CCNGG 6 cut(s) 36, 235, 251, 265, 309, 455
SduI GDGCHC 1 cut(s) 367
Sfr274I CTCGAG 1 cut(s) 410
SlaI CTCGAG 1 cut(s) 410
SmiMI CAYNNNNRTG 1 cut(s) 147
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
Sse9I AATT 3 cut(s) 132, 375, 673
SstI GAGCTC 1 cut(s) 367
StyD4I CCNGG 6 cut(s) 34, 233, 249, 263, 307, 453
StyI CCWWGG 2 cut(s) 155, 432
TaaI ACNGT 1 cut(s) 590
TaqI TCGA 2 cut(s) 43, 411
TasI AATT 3 cut(s) 132, 375, 673
TfiI GAWTC 2 cut(s) 45, 778
Tru1I TTAA 2 cut(s) 378, 551
Tru9I TTAA 2 cut(s) 378, 551
TscAI CASTG 3 cut(s) 33, 547, 593
TseFI GTSAC 2 cut(s) 94, 215
TseI GCWGC 2 cut(s) 160, 545
Tsp45I GTSAC 2 cut(s) 94, 215
TspDTI ATGAA 3 cut(s) 497, 509, 791
TspRI CASTG 3 cut(s) 33, 547, 593
XceI RCATGY 2 cut(s) 427, 586
XhoI CTCGAG 1 cut(s) 410
Zsp2I ATGCAT 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.