Rorug01G0362100

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001617
Physical Location & Seq
Forward (+)
47754891 .. 47757572
2682 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0362100.1

Sequence Viewer

Length: 972 bp
ATGCCCCTCCCTCAGGACCCCCTGGACCTCCCTCTCGACGCCCTCATCGCCGAATCCAGGCAACAACGCCGTCACAACAACCAGGCTCACCACCCACGAGGGCATACCGGTCATTTCGGTGGCAGAGGCTCCACCTCAGGCCCCGCTCGTCGGCCGATGAACCGCAACGCCTACAGAACGGCGCCGTATCCCACTCTGCCGGAAATGCAAGGACAGCCTGGACTCAGAAGGATTGAGCTGCCATTGGCTTTGAGCCAAGCGATGAACAGAGAGGGGAGCACCAAGCTTTATATATCTAACTTGGACTATGATGTTACCAATAGAGATATAGAGTTGCTCTTCTCTGAGGTTGGTGAGTTGGAAAGGCACTCAATCCATTATGATAAAAGTGGAAGATCTAAGGGAACAGCTGAAGTTGTCTATAAGCACCATTCAGATGCTCTAACAGCAATTGAGAGATATAACAATCAGCATCTTGATGGAAAGAGAGTGGAAATTAAGCTTGTGGGACTCAAAGTTGTTTCTCCTGTTCCTCTGCTTCTGCCTCCAAATACAAATTTCTTGCAAGAAAATCCAAGTCCTGTCTTCCAGAGTCTGAAACTATTGAAGGAACGTGCTAAAGAGAGGGAGAACATTATTCATGGAAGTGAAAGAAATGTGATGACTGATGAGCTAGGAAGAGCTGGTTCAAGGGGATCATTTCATGGTCATGGTGGTGTTGGACTTGAAAGAGGCCGTGGACAGGTGAGAAATGGTGGTCCGAAGGTTACTACAGAATCTCAGCAATGGAAGAATGTGACTACAGAATCTCAGCAAAGGAAGAATGTGACTGCAGAAAATCAGCAAAGGAAGAATGTGATTACAGAATCTGTTAACAGGGAGAAAACTTTTTATGGAGATACAACAGTGACTTTTGAAGATCTGGATGCTGATTTGGAGAAATACCGCCTAAAAGGTAGGCCGGTAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

323

Amino Acids

36.41

Weight (kDa)

9.56

Isoelectric Point (pI)

48.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 96 - 164 2.4e-15 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 181
AccBSI CCGCTC 1 cut(s) 146
AciI CCGC 3 cut(s) 144, 163, 946
AclWI GGATC 1 cut(s) 703
AcoI YGGCCR 1 cut(s) 152
AcsI RAATTY 1 cut(s) 556
AcuI CTGAAG 1 cut(s) 432
AcyI GRCGYC 2 cut(s) 39, 182
AfiI CCNNNNNNNGG 4 cut(s) 13, 57, 150, 742
AgeI ACCGGT 1 cut(s) 107
AgsI TTSAA 4 cut(s) 607, 690, 728, 917
AjnI CCWGG 4 cut(s) 21, 56, 81, 217
AluBI AGCT 6 cut(s) 238, 286, 410, 502, 673, 683
AluI AGCT 6 cut(s) 238, 286, 410, 502, 673, 683
Alw21I GWGCWC 1 cut(s) 281
AlwI GGATC 1 cut(s) 703
AlwNI CAGNNNCTG 2 cut(s) 595, 869
AoxI GGCC 4 cut(s) 139, 152, 733, 959
ApeKI GCWGC 1 cut(s) 238
ApoI RAATTY 1 cut(s) 556
AsiGI ACCGGT 1 cut(s) 107
AspLEI GCGC 1 cut(s) 184
AspS9I GGNCC 4 cut(s) 16, 25, 140, 758
AsuHPI GGTGA 3 cut(s) 80, 365, 757
AvaII GGWCC 3 cut(s) 16, 25, 758
AxyI CCTNAGG 2 cut(s) 12, 136
BaeI ACNNNNGTAYC 2 cut(s) 891, 924
BanI GGYRCC 1 cut(s) 181
BauI CACGAG 1 cut(s) 96
BbsI GAAGAC 1 cut(s) 577
Bbv12I GWGCWC 1 cut(s) 281
BbvI GCAGC 1 cut(s) 225
BccI CCATC 1 cut(s) 473
BceAI ACGGC 4 cut(s) 54, 169, 195, 720
BciT130I CCWGG 4 cut(s) 23, 58, 83, 219
BciVI GTATCC 1 cut(s) 198
BfaI CTAG 1 cut(s) 674
BfmI CTRYAG 4 cut(s) 172, 771, 801, 831
BfoI RGCGCY 1 cut(s) 185
BfuI GTATCC 1 cut(s) 198
BglII AGATCT 2 cut(s) 395, 919
BisI GCNGC 1 cut(s) 239
BlsI GCNGC 1 cut(s) 240
Bme1390I CCNGG 4 cut(s) 23, 58, 83, 219
Bme18I GGWCC 3 cut(s) 16, 25, 758
BmgT120I GGNCC 4 cut(s) 16, 25, 140, 758
BmiI GGNNCC 4 cut(s) 18, 130, 142, 183
BmrFI CCNGG 4 cut(s) 23, 58, 83, 219
BmsI GCATC 3 cut(s) 427, 481, 916
BpiI GAAGAC 1 cut(s) 577
BsaHI GRCGYC 2 cut(s) 39, 182
BsaJI CCNNGG 2 cut(s) 21, 736
BsaWI WCCGGW 1 cut(s) 107
Bsc4I CCNNNNNNNGG 4 cut(s) 13, 57, 150, 742
Bse118I RCCGGY 2 cut(s) 107, 961
Bse21I CCTNAGG 2 cut(s) 12, 136
Bse3DI GCAATG 1 cut(s) 791
BseBI CCWGG 4 cut(s) 23, 58, 83, 219
BseDI CCNNGG 2 cut(s) 21, 736
BseGI GGATG 1 cut(s) 931
BseLI CCNNNNNNNGG 4 cut(s) 13, 57, 150, 742
BseMI GCAATG 1 cut(s) 791
BseMII CTCAG 6 cut(s) 26, 150, 238, 336, 794, 824
BseX3I CGGCCG 1 cut(s) 152
BseXI GCAGC 1 cut(s) 225
Bsh1285I CGRYCG 1 cut(s) 155
BshFI GGCC 4 cut(s) 141, 154, 735, 961
BshNI GGYRCC 1 cut(s) 181
BshTI ACCGGT 1 cut(s) 107
BsiEI CGRYCG 1 cut(s) 155
BsiHKAI GWGCWC 1 cut(s) 281
BsiSI CCGG 3 cut(s) 108, 200, 962
BslFI GGGAC 1 cut(s) 522
BslI CCNNNNNNNGG 4 cut(s) 13, 57, 150, 742
BsmFI GGGAC 1 cut(s) 522
BsnI GGCC 4 cut(s) 141, 154, 735, 961
Bsp1286I GDGCHC 1 cut(s) 281
Bsp143I GATC 3 cut(s) 395, 695, 919
BspACI CCGC 3 cut(s) 144, 163, 946
BspANI GGCC 4 cut(s) 141, 154, 735, 961
BspCNI CTCAG 6 cut(s) 25, 149, 237, 337, 793, 823
BspLI GGNNCC 4 cut(s) 18, 130, 142, 183
BspMAI CTGCAG 1 cut(s) 835
BspPI GGATC 1 cut(s) 703
BspQI GCTCTTC 2 cut(s) 344, 673
BspT107I GGYRCC 1 cut(s) 181
BsrBI CCGCTC 1 cut(s) 146
BsrDI GCAATG 1 cut(s) 791
BsrFI RCCGGY 2 cut(s) 107, 961
BssAI RCCGGY 2 cut(s) 107, 961
BssECI CCNNGG 2 cut(s) 21, 736
BssMI GATC 3 cut(s) 395, 695, 919
BssNI GRCGYC 2 cut(s) 39, 182
BssSI CACGAG 1 cut(s) 96
Bst2BI CACGAG 1 cut(s) 96
Bst2UI CCWGG 4 cut(s) 23, 58, 83, 219
Bst4CI ACNGT 1 cut(s) 907
Bst6I CTCTTC 2 cut(s) 344, 673
BstACI GRCGYC 2 cut(s) 39, 182
BstDEI CTNAG 7 cut(s) 12, 136, 224, 345, 399, 780, 810
BstDSI CCRYGG 1 cut(s) 736
BstENI CCTNNNNNAGG 1 cut(s) 11
BstF5I GGATG 1 cut(s) 931
BstH2I RGCGCY 1 cut(s) 185
BstHHI GCGC 1 cut(s) 184
BstKTI GATC 3 cut(s) 398, 698, 922
BstMBI GATC 3 cut(s) 395, 695, 919
BstMCI CGRYCG 1 cut(s) 155
BstMWI GCNNNNNNNGC 4 cut(s) 47, 205, 214, 446
BstNI CCWGG 4 cut(s) 23, 58, 83, 219
BstSCI CCNGG 4 cut(s) 21, 56, 81, 217
BstSFI CTRYAG 4 cut(s) 172, 771, 801, 831
BstV1I GCAGC 1 cut(s) 225
BstV2I GAAGAC 1 cut(s) 577
BstX2I RGATCY 2 cut(s) 395, 919
BstYI RGATCY 2 cut(s) 395, 919
BstZI CGGCCG 1 cut(s) 152
Bsu36I CCTNAGG 2 cut(s) 12, 136
BsuI GTATCC 1 cut(s) 198
BsuRI GGCC 4 cut(s) 141, 154, 735, 961
BtgI CCRYGG 1 cut(s) 736
BtgZI GCGATG 2 cut(s) 31, 275
BtsCI GGATG 1 cut(s) 931
BtsIMutI CAGTG 1 cut(s) 912
CaiI CAGNNNCTG 2 cut(s) 595, 869
CfoI GCGC 1 cut(s) 184
Cfr10I RCCGGY 2 cut(s) 107, 961
Cfr13I GGNCC 4 cut(s) 16, 25, 140, 758
CseI GACGC 1 cut(s) 47
CspAI ACCGGT 1 cut(s) 107
CviAII CATG 3 cut(s) 641, 704, 710
DdeI CTNAG 7 cut(s) 12, 136, 224, 345, 399, 780, 810
DinI GGCGCC 1 cut(s) 183
DpnI GATC 3 cut(s) 397, 697, 921
DpnII GATC 3 cut(s) 395, 695, 919
EaeI YGGCCR 1 cut(s) 152
EagI CGGCCG 1 cut(s) 152
Eam1104I CTCTTC 2 cut(s) 344, 673
EarI CTCTTC 2 cut(s) 344, 673
EclXI CGGCCG 1 cut(s) 152
Eco47I GGWCC 3 cut(s) 16, 25, 758
Eco52I CGGCCG 1 cut(s) 152
Eco57I CTGAAG 1 cut(s) 432
Eco81I CCTNAGG 2 cut(s) 12, 136
EcoNI CCTNNNNNAGG 1 cut(s) 11
EcoO109I RGGNCCY 2 cut(s) 16, 140
EcoRII CCWGG 4 cut(s) 21, 56, 81, 217
EgeI GGCGCC 1 cut(s) 183
EheI GGCGCC 1 cut(s) 183
FaeI CATG 3 cut(s) 644, 707, 713
FaqI GGGAC 1 cut(s) 522
FatI CATG 3 cut(s) 640, 703, 709
FauI CCCGC 1 cut(s) 151
Fnu4HI GCNGC 1 cut(s) 239
FokI GGATG 1 cut(s) 938
Fsp4HI GCNGC 1 cut(s) 239
FspBI CTAG 1 cut(s) 674
GlaI GCGC 1 cut(s) 183
GluI GCNGC 1 cut(s) 239
HaeII RGCGCY 1 cut(s) 185
HaeIII GGCC 4 cut(s) 141, 154, 735, 961
HapII CCGG 3 cut(s) 108, 200, 962
HgaI GACGC 1 cut(s) 47
HhaI GCGC 1 cut(s) 184
Hin1I GRCGYC 2 cut(s) 39, 182
Hin1II CATG 3 cut(s) 644, 707, 713
Hin6I GCGC 1 cut(s) 182
HinP1I GCGC 1 cut(s) 182
HincII GTYRAC 1 cut(s) 874
HindII GTYRAC 1 cut(s) 874
HindIII AAGCTT 2 cut(s) 284, 500
HinfI GANTC 7 cut(s) 53, 222, 510, 592, 776, 806, 866
HpaI GTTAAC 1 cut(s) 874
HpaII CCGG 3 cut(s) 108, 200, 962
HphI GGTGA 3 cut(s) 80, 365, 757
Hpy166II GTNNAC 3 cut(s) 740, 874, 967
Hpy188I TCNGA 5 cut(s) 227, 346, 436, 597, 762
Hpy188III TCNNGA 5 cut(s) 14, 35, 476, 589, 923
Hpy8I GTNNAC 3 cut(s) 740, 874, 967
Hpy99I CGWCG 2 cut(s) 41, 153
HpyAV CCTTC 3 cut(s) 222, 601, 757
HpyCH4III ACNGT 1 cut(s) 907
HpyCH4IV ACGT 1 cut(s) 613
HpyCH4V TGCA 3 cut(s) 208, 565, 833
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 205, 214, 446
HpyF3I CTNAG 7 cut(s) 12, 136, 224, 345, 399, 780, 810
HpySE526I ACGT 1 cut(s) 613
Hsp92I GRCGYC 2 cut(s) 39, 182
Hsp92II CATG 3 cut(s) 644, 707, 713
HspAI GCGC 1 cut(s) 182
KasI GGCGCC 1 cut(s) 181
KspAI GTTAAC 1 cut(s) 874
Kzo9I GATC 3 cut(s) 395, 695, 919
LguI GCTCTTC 2 cut(s) 344, 673
LmnI GCTCC 2 cut(s) 134, 276
Lsp1109I GCAGC 1 cut(s) 225
LweI GCATC 3 cut(s) 427, 481, 916
MaeI CTAG 1 cut(s) 674
MaeII ACGT 1 cut(s) 613
MaeIII GTNAC 6 cut(s) 71, 313, 766, 796, 826, 907
MalI GATC 3 cut(s) 397, 697, 921
MbiI CCGCTC 1 cut(s) 146
MboI GATC 3 cut(s) 395, 695, 919
MboII GAAGA 8 cut(s) 331, 405, 577, 690, 802, 832, 862, 929
MfeI CAATTG 1 cut(s) 450
MflI RGATCY 2 cut(s) 395, 919
MhlI GDGCHC 1 cut(s) 281
MluCI AATT 3 cut(s) 450, 495, 556
Mly113I GGCGCC 1 cut(s) 182
MlyI GAGTC 3 cut(s) 216, 504, 601
MmeI TCCRAC 2 cut(s) 339, 700
MseI TTAA 2 cut(s) 498, 873
MslI CAYNNNNRTG 6 cut(s) 117, 435, 477, 645, 708, 714
MspA1I CMGCKG 1 cut(s) 410
MspI CCGG 3 cut(s) 108, 200, 962
MspR9I CCNGG 4 cut(s) 23, 58, 83, 219
MunI CAATTG 1 cut(s) 450
MvaI CCWGG 4 cut(s) 23, 58, 83, 219
MwoI GCNNNNNNNGC 4 cut(s) 47, 205, 214, 446
NarI GGCGCC 1 cut(s) 182
NdeII GATC 3 cut(s) 395, 695, 919
NlaIII CATG 3 cut(s) 644, 707, 713
NlaIV GGNNCC 4 cut(s) 18, 130, 142, 183
NmuCI GTSAC 4 cut(s) 71, 796, 826, 907
PciSI GCTCTTC 2 cut(s) 344, 673
PfeI GAWTC 4 cut(s) 53, 776, 806, 866
PinAI ACCGGT 1 cut(s) 107
PkrI GCNGC 1 cut(s) 240
PleI GAGTC 3 cut(s) 216, 504, 600
PluTI GGCGCC 1 cut(s) 185
PpsI GAGTC 3 cut(s) 216, 504, 600
PpuMI RGGWCCY 1 cut(s) 16
Psp5II RGGWCCY 1 cut(s) 16
Psp6I CCWGG 4 cut(s) 21, 56, 81, 217
PspGI CCWGG 4 cut(s) 21, 56, 81, 217
PspN4I GGNNCC 4 cut(s) 18, 130, 142, 183
PspPI GGNCC 4 cut(s) 16, 25, 140, 758
PspPPI RGGWCCY 1 cut(s) 16
PstI CTGCAG 1 cut(s) 835
PstNI CAGNNNCTG 2 cut(s) 595, 869
PsuI RGATCY 2 cut(s) 395, 919
PvuII CAGCTG 1 cut(s) 410
RseI CAYNNNNRTG 6 cut(s) 117, 435, 477, 645, 708, 714
SapI GCTCTTC 2 cut(s) 344, 673
SaqAI TTAA 2 cut(s) 498, 873
SatI GCNGC 1 cut(s) 239
Sau3AI GATC 3 cut(s) 395, 695, 919
Sau96I GGNCC 4 cut(s) 16, 25, 140, 758
SchI GAGTC 3 cut(s) 216, 504, 601
ScrFI CCNGG 4 cut(s) 23, 58, 83, 219
SduI GDGCHC 1 cut(s) 281
SfaNI GCATC 3 cut(s) 427, 481, 916
SfcI CTRYAG 4 cut(s) 172, 771, 801, 831
SfoI GGCGCC 1 cut(s) 183
SinI GGWCC 3 cut(s) 16, 25, 758
SmiMI CAYNNNNRTG 6 cut(s) 117, 435, 477, 645, 708, 714
Sse9I AATT 3 cut(s) 450, 495, 556
SsiI CCGC 3 cut(s) 144, 163, 946
SspDI GGCGCC 1 cut(s) 181
SspMI CTAG 1 cut(s) 674
StyD4I CCNGG 4 cut(s) 21, 56, 81, 217
TaaI ACNGT 1 cut(s) 907
TaiI ACGT 1 cut(s) 616
TaqI TCGA 1 cut(s) 36
TasI AATT 3 cut(s) 450, 495, 556
TfiI GAWTC 4 cut(s) 53, 776, 806, 866
Tru1I TTAA 2 cut(s) 498, 873
Tru9I TTAA 2 cut(s) 498, 873
TscAI CASTG 1 cut(s) 912
TseFI GTSAC 4 cut(s) 71, 796, 826, 907
TseI GCWGC 1 cut(s) 238
Tsp45I GTSAC 4 cut(s) 71, 796, 826, 907
TspDTI ATGAA 4 cut(s) 173, 278, 629, 692
TspRI CASTG 1 cut(s) 912
VpaK11BI GGWCC 3 cut(s) 16, 25, 758
XagI CCTNNNNNAGG 1 cut(s) 11
XapI RAATTY 1 cut(s) 556
XspI CTAG 1 cut(s) 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.