Rorug03G0293500

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001617
Physical Location & Seq
Reverse (-)
30066370 .. 30070568
4199 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0293500.1

Sequence Viewer

Length: 2190 bp
ATGGGTACGGAGAGGAAGAGGAAGGTGAGCTTGTTCGACGTGGTGGACGAGACCACTGTTGCAGCTGCGAAGATGAGCAAATCGAACGGTGGTGGAGGCGCCATGAATCACAACAACCCGATGAGCAGTCTGATCAATAGGTGGACCGGAAAGCCTTTCTCGCAAAGGTACTATGAGATCCTTGAGAAGAGGAAGACTTTGCCTGTTTGGCACCAGAAGGAGGAGTTCTTTCAGGTTCTCAAGAAAAGTCAGTCGCTGATTCTGGTTGGTGAGACTGGTAGTGGTAAAACCACTCAGATTCCTCAATTTGTTTTGGAAGCTGTTGATAGGGAGTCTTCAGATAAAAGCAGGAAGATGATTGCATGTACCCAGCCTCGTAGGGTGGCTGCAATGTCAGTGTCTCGCCGTGTTGCTGAAGAGATGGATGTTACCATTGGGGAAGAGGTTGGTTACAGCATTCGTTTTGAAGACTGCAGCAGTGCCAGAACAGTATTGAAGTATCTTACAGATGGTATGCTTTTAAGAGAAGCCATGACAGATCCACTATTAGAACGATACAGTGTAATAATTCTTGATGAGGCTCATGAGAGGACTTTAGCAACAGATGTTCTGTTTGGGCTTCTGAAGGAAGTATTGAAAAATAGACCTGATATGAAGCTGGTTGTTATGAGTGCTACACTTGAGGCTGAGAAGTTTCAGGGTTATTTCAACGGTGCACCACTAATGAAGGTTCCTGGCAGGCTTCATCCAGTTGAAATTTTTTACACCGAGGAACCTGAGAGGGACTACCTGGAGGCAGCAATTCGAACTGTTGTACAGATTCATATGTATGAGACTCCAGGAGATATACTTGTTTTTTTAACTGGAGAAGAGGAGATAGAAGATGCATGCCGCAAAATCAACAAAGAAGTTTCAAACCTGGGTGATCAGGTTGGTCCAGTGAAAGTGGTGCCCCTGTATTCAACTCTCCCTCCAGCTATGCAGCAGAAAATATTTGATGCTGCTCCTCCTCCCGCAACAGAAGGCGGTCCTGCAGGAAGGAAAATTGTGGTGTCAACTAACATTGCAGAAACTTCTCTGACCATAGATGGGATCGTGTATGTTATTGATCCAGGTTTTTCTAAACAGAAAGTTTATAACCCACGAGTGCGTGTTGAATCCTTGTTGGTATCTCCGATTTCTAAGGCTAGTGCACATCAGAGGTCAGGGCGTGCTGGAAGAACTCAGCCAGGCAAATGTTTCAGACTTTATACTGAGAAAAGTTTCCATAATGATCTTGTACCGCAGACATATCCAGAAATACTGAGATCAAATCTTGCAAATACAGTGCTTACTTTGAAGAAACTAGGAATAGATGATTTGGTTCATTTTGATTTTATGGATCCCCCTGCTCCAGAGACATTAATGAGAGCACTCGAGGTTTTGAATTATTTGGGTGCATTGGATGATGATGGTAACTTGACCAAGCTGGGTGAGATTATGAGCGAGTTCCCTCTGGATCCTCAGATGTCAAAGATGCTTGTTGTTAGCCCTGAGTTTAACTGCTCAAACGAGATTTTGTCGATTTCTGCCATGCTTTCAGTACCCAATTGCTTTGTCCGGCCTAGGGAGGCTCAGAAAGCTGCAGATGAAGCAAAAGCTAGGTTTGGACACATCGATGGAGATCATCTCACACTGCTGAATGTGTATCATGCATACAAGCAAAACAATGAAGATCCATCGTGGTGCTATGAGAACTTTGTCAATCAGAGGGCATTGAAGTCTGCAGACAATGTTAGACAACAGCTAGTGCGCATTATGGCTCGGTTTAATCTCAAGCTATGCAGTACTGATTTTAAGAGTCGGGACTACTACATCAACATCAGAAAGGCTATGCTAGCTGGGTACTTCATGCAGGTGGCTCATCTGGAACGCACTGGTCACTACCTTACTGTGAAGGATAACCAAGTTGTACATTTACATCCATCAAATTGCTTGGATCACAAGCCAGAGTGGGTGATTTACAATGAGTACGTCTTAACGAGCAGGAATTTTATCCGCACAGTGACAGATATCCGTGGTGAATGGCTAGTTGATATAGCACCACACTACTATGATTTGGCCAACTTTCCCCAGTGTGAGGCCAAGCGAGTTCTTGAGAAGCTTTACAGGAAGCGGGACGACAAGGAGGATAACAGAAACAGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

729

Amino Acids

82.82

Weight (kDa)

7.0

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 73 - 228 6.1e-10 DEAD/DEAH box helicase
AAA_22 PF13401 83 - 213 1.6e-07 AAA domain
Helicase_C PF00271 269 - 408 1.6e-09 Helicase conserved C-terminal domain
WHD_HA2 PF04408 471 - 499 2.3e-11 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 500 - 559 1.1e-14 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 621 - 697 8.6e-25 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1137
AarI CACCTGC 1 cut(s) 1885
Acc16I TGCGCA 1 cut(s) 1793
Acc36I ACCTGC 1 cut(s) 1885
AccB1I GGYRCC 3 cut(s) 98, 210, 949
AciI CCGC 6 cut(s) 892, 1014, 1026, 1283, 2038, 2155
AcoI YGGCCR 1 cut(s) 2100
AcsI RAATTY 2 cut(s) 756, 2029
AcuI CTGAAG 3 cut(s) 321, 435, 644
AcyI GRCGYC 1 cut(s) 99
AfiI CCNNNNNNNGG 4 cut(s) 220, 1089, 1606, 2119
AjiI CACGTC 1 cut(s) 40
AjnI CCWGG 6 cut(s) 733, 789, 838, 918, 1111, 1228
Alw21I GWGCWC 3 cut(s) 718, 1195, 1414
Alw26I GTCTC 5 cut(s) 44, 266, 405, 827, 1391
Alw44I GTGCAC 2 cut(s) 714, 1191
AlwNI CAGNNNCTG 1 cut(s) 256
Ama87I CYCGRG 1 cut(s) 1415
AoxI GGCC 3 cut(s) 1601, 2100, 2121
ApaLI GTGCAC 2 cut(s) 714, 1191
ApeKI GCWGC 8 cut(s) 62, 65, 386, 474, 797, 982, 1001, 1622
ApoI RAATTY 2 cut(s) 756, 2029
AseI ATTAAT 1 cut(s) 1403
Asp700I GAANNNNTTC 1 cut(s) 1262
AspA2I CCTAGG 1 cut(s) 1604
AspLEI GCGC 2 cut(s) 101, 1794
AspS9I GGNCC 3 cut(s) 144, 935, 1028
AsuHPI GGTGA 6 cut(s) 37, 281, 935, 1484, 2008, 2072
AsuII TTCGAA 1 cut(s) 805
AsuNHI GCTAGC 1 cut(s) 1876
AvaI CYCGRG 1 cut(s) 1415
AvaII GGWCC 3 cut(s) 144, 935, 1028
AvrII CCTAGG 1 cut(s) 1604
BaeGI GKGCMC 3 cut(s) 718, 954, 1195
BalI TGGCCA 1 cut(s) 2102
BamHI GGATCC 2 cut(s) 1381, 1498
BanI GGYRCC 3 cut(s) 98, 210, 949
BauI CACGAG 1 cut(s) 1143
BbsI GAAGAC 3 cut(s) 200, 327, 474
Bbv12I GWGCWC 3 cut(s) 718, 1195, 1414
BbvI GCAGC 8 cut(s) 52, 74, 373, 486, 809, 988, 994, 1609
BccI CCATC 7 cut(s) 415, 503, 1082, 1445, 1652, 1726, 1972
BceAI ACGGC 1 cut(s) 390
BciT130I CCWGG 6 cut(s) 735, 791, 840, 920, 1113, 1230
BclI TGATCA 2 cut(s) 132, 925
BcoDI GTCTC 5 cut(s) 44, 266, 405, 827, 1391
BfaI CTAG 7 cut(s) 1188, 1346, 1605, 1641, 1787, 1877, 2069
BfmI CTRYAG 4 cut(s) 472, 1032, 1623, 1764
BfoI RGCGCY 1 cut(s) 102
BfuAI ACCTGC 1 cut(s) 1885
BglI GCCNNNNNGGC 1 cut(s) 208
BisI GCNGC 9 cut(s) 63, 66, 387, 475, 798, 892, 983, 1002, 1623
BlnI CCTAGG 1 cut(s) 1604
BlsI GCNGC 9 cut(s) 64, 67, 388, 476, 799, 893, 984, 1003, 1624
BmcAI AGTACT 1 cut(s) 1828
Bme1390I CCNGG 6 cut(s) 735, 791, 840, 920, 1113, 1230
Bme18I GGWCC 3 cut(s) 144, 935, 1028
BmeT110I CYCGRG 1 cut(s) 1415
BmgBI CACGTC 1 cut(s) 40
BmgT120I GGNCC 3 cut(s) 144, 935, 1028
BmiI GGNNCC 7 cut(s) 100, 212, 732, 774, 951, 1383, 1500
BmrFI CCNGG 6 cut(s) 735, 791, 840, 920, 1113, 1230
BmrI ACTGGG 1 cut(s) 2107
BmsI GCATC 3 cut(s) 874, 988, 1506
BmtI GCTAGC 1 cut(s) 1880
BmuI ACTGGG 1 cut(s) 2107
BpiI GAAGAC 3 cut(s) 200, 327, 474
BplI GAGNNNNNCTC 2 cut(s) 1653, 1685
BpmI CTGGAG 5 cut(s) 812, 822, 885, 957, 1377
Bpu14I TTCGAA 1 cut(s) 805
BpuEI CTTGAG 5 cut(s) 203, 224, 701, 1799, 2156
Bsa29I ATCGAT 1 cut(s) 1656
BsaBI GATNNNNATC 1 cut(s) 1662
BsaHI GRCGYC 1 cut(s) 99
BsaI GGTCTC 1 cut(s) 44
BsaJI CCNNGG 4 cut(s) 768, 919, 1604, 2056
BsaWI WCCGGW 1 cut(s) 146
BsaXI ACNNNNNCTCC 2 cut(s) 955, 985
Bsc4I CCNNNNNNNGG 4 cut(s) 220, 1089, 1606, 2119
Bse1I ACTGG 6 cut(s) 280, 749, 868, 938, 1921, 2113
Bse3DI GCAATG 2 cut(s) 396, 1062
Bse8I GATNNNNATC 1 cut(s) 1662
BseBI CCWGG 6 cut(s) 735, 791, 840, 920, 1113, 1230
BseCI ATCGAT 1 cut(s) 1656
BseDI CCNNGG 4 cut(s) 768, 919, 1604, 2056
BseGI GGATG 4 cut(s) 430, 745, 1450, 1960
BseJI GATNNNNATC 1 cut(s) 1662
BseLI CCNNNNNNNGG 4 cut(s) 220, 1089, 1606, 2119
BseMI GCAATG 2 cut(s) 396, 1062
BseMII CTCAG 9 cut(s) 308, 678, 768, 1238, 1245, 1295, 1517, 1524, 1628
BseNI ACTGG 6 cut(s) 280, 749, 868, 938, 1921, 2113
BseRI GAGGAG 4 cut(s) 236, 887, 996, 999
BseSI GKGCMC 3 cut(s) 718, 954, 1195
BseXI GCAGC 8 cut(s) 52, 74, 373, 486, 809, 988, 994, 1609
BseYI CCCAGC 3 cut(s) 369, 1468, 1880
BshFI GGCC 3 cut(s) 1603, 2102, 2123
BshNI GGYRCC 3 cut(s) 98, 210, 949
BshVI ATCGAT 1 cut(s) 1656
BsiHKAI GWGCWC 3 cut(s) 718, 1195, 1414
BsiHKCI CYCGRG 1 cut(s) 1415
BsiSI CCGG 2 cut(s) 147, 1600
BslFI GGGAC 3 cut(s) 797, 1859, 2171
BslI CCNNNNNNNGG 4 cut(s) 220, 1089, 1606, 2119
BsmAI GTCTC 5 cut(s) 44, 266, 405, 827, 1391
BsmFI GGGAC 3 cut(s) 797, 1859, 2171
BsmI GAATGC 1 cut(s) 456
BsnI GGCC 3 cut(s) 1603, 2102, 2123
Bso31I GGTCTC 1 cut(s) 44
BsoBI CYCGRG 1 cut(s) 1415
Bsp119I TTCGAA 1 cut(s) 805
Bsp1286I GDGCHC 4 cut(s) 718, 954, 1195, 1414
Bsp1407I TGTACA 2 cut(s) 814, 1951
BspACI CCGC 6 cut(s) 892, 1014, 1026, 1283, 2038, 2155
BspANI GGCC 3 cut(s) 1603, 2102, 2123
BspCNI CTCAG 9 cut(s) 307, 679, 769, 1237, 1246, 1296, 1516, 1525, 1627
BspDI ATCGAT 1 cut(s) 1656
BspHI TCATGA 1 cut(s) 583
BspLI GGNNCC 7 cut(s) 100, 212, 732, 774, 951, 1383, 1500
BspMAI CTGCAG 4 cut(s) 476, 1036, 1627, 1768
BspMI ACCTGC 1 cut(s) 1885
BspOI GCTAGC 1 cut(s) 1880
BspT104I TTCGAA 1 cut(s) 805
BspT107I GGYRCC 3 cut(s) 98, 210, 949
BspTNI GGTCTC 1 cut(s) 44
BsrDI GCAATG 2 cut(s) 396, 1062
BsrGI TGTACA 2 cut(s) 814, 1951
BsrI ACTGG 6 cut(s) 280, 749, 868, 938, 1921, 2113
BssECI CCNNGG 4 cut(s) 768, 919, 1604, 2056
BssNI GRCGYC 1 cut(s) 99
BssSI CACGAG 1 cut(s) 1143
BssT1I CCWWGG 1 cut(s) 1604
Bst2BI CACGAG 1 cut(s) 1143
Bst2UI CCWGG 6 cut(s) 735, 791, 840, 920, 1113, 1230
Bst4CI ACNGT 9 cut(s) 58, 89, 490, 560, 713, 811, 1327, 1933, 2044
Bst6I CTCTTC 5 cut(s) 11, 182, 411, 435, 864
BstACI GRCGYC 1 cut(s) 99
BstAUI TGTACA 2 cut(s) 814, 1951
BstBI TTCGAA 1 cut(s) 805
BstC8I GCNNGC 4 cut(s) 740, 889, 1212, 1878
BstDSI CCRYGG 1 cut(s) 2056
BstF5I GGATG 4 cut(s) 430, 745, 1450, 1960
BstH2I RGCGCY 1 cut(s) 102
BstHHI GCGC 2 cut(s) 101, 1794
BstMAI GTCTC 5 cut(s) 44, 266, 405, 827, 1391
BstMWI GCNNNNNNNGC 5 cut(s) 160, 208, 1619, 1631, 1877
BstNI CCWGG 6 cut(s) 735, 791, 840, 920, 1113, 1230
BstNSI RCATGY 2 cut(s) 366, 891
BstSCI CCNGG 6 cut(s) 733, 789, 838, 918, 1111, 1228
BstSFI CTRYAG 4 cut(s) 472, 1032, 1623, 1764
BstSLI GKGCMC 3 cut(s) 718, 954, 1195
BstV1I GCAGC 8 cut(s) 52, 74, 373, 486, 809, 988, 994, 1609
BstV2I GAAGAC 3 cut(s) 200, 327, 474
BstX2I RGATCY 5 cut(s) 177, 538, 1381, 1498, 1714
BstYI RGATCY 5 cut(s) 177, 538, 1381, 1498, 1714
Bsu15I ATCGAT 1 cut(s) 1656
BsuRI GGCC 3 cut(s) 1603, 2102, 2123
BsuTUI ATCGAT 1 cut(s) 1656
BtgI CCRYGG 1 cut(s) 2056
BtrI CACGTC 1 cut(s) 40
BtsCI GGATG 4 cut(s) 430, 745, 1450, 1960
BtsI GCAGTG 2 cut(s) 484, 1673
BveI ACCTGC 1 cut(s) 1885
Cac8I GCNNGC 4 cut(s) 740, 889, 1212, 1878
CaiI CAGNNNCTG 1 cut(s) 256
CciI TCATGA 1 cut(s) 583
CfoI GCGC 2 cut(s) 101, 1794
Cfr13I GGNCC 3 cut(s) 144, 935, 1028
ClaI ATCGAT 1 cut(s) 1656
CviAII CATG 8 cut(s) 103, 363, 532, 584, 888, 1573, 1691, 1891
DinI GGCGCC 1 cut(s) 100
EaeI YGGCCR 1 cut(s) 2100
Eam1104I CTCTTC 5 cut(s) 11, 182, 411, 435, 864
EarI CTCTTC 5 cut(s) 11, 182, 411, 435, 864
Eco130I CCWWGG 1 cut(s) 1604
Eco31I GGTCTC 1 cut(s) 44
Eco32I GATATC 1 cut(s) 2053
Eco47I GGWCC 3 cut(s) 144, 935, 1028
Eco57I CTGAAG 3 cut(s) 321, 435, 644
Eco88I CYCGRG 1 cut(s) 1415
EcoRII CCWGG 6 cut(s) 733, 789, 838, 918, 1111, 1228
EcoRV GATATC 1 cut(s) 2053
EcoT14I CCWWGG 1 cut(s) 1604
EcoT22I ATGCAT 2 cut(s) 889, 1696
EgeI GGCGCC 1 cut(s) 100
EheI GGCGCC 1 cut(s) 100
ErhI CCWWGG 1 cut(s) 1604
FaeI CATG 8 cut(s) 106, 366, 535, 587, 891, 1576, 1694, 1894
FalI AAGNNNNNCTT 2 cut(s) 14, 46
FaqI GGGAC 3 cut(s) 797, 1859, 2171
FatI CATG 8 cut(s) 102, 362, 531, 583, 887, 1572, 1690, 1890
FauI CCCGC 2 cut(s) 1021, 2148
FauNDI CATATG 1 cut(s) 825
FbaI TGATCA 2 cut(s) 132, 925
Fnu4HI GCNGC 9 cut(s) 63, 66, 387, 475, 798, 892, 983, 1002, 1623
FokI GGATG 4 cut(s) 437, 732, 1457, 1947
Fsp4HI GCNGC 9 cut(s) 63, 66, 387, 475, 798, 892, 983, 1002, 1623
FspAI RTGCGCAY 1 cut(s) 1793
FspBI CTAG 7 cut(s) 1188, 1346, 1605, 1641, 1787, 1877, 2069
FspI TGCGCA 1 cut(s) 1793
GlaI GCGC 2 cut(s) 100, 1793
GluI GCNGC 9 cut(s) 63, 66, 387, 475, 798, 892, 983, 1002, 1623
GsaI CCCAGC 3 cut(s) 373, 1472, 1884
GsuI CTGGAG 5 cut(s) 812, 822, 885, 957, 1377
HaeII RGCGCY 1 cut(s) 102
HaeIII GGCC 3 cut(s) 1603, 2102, 2123
HapII CCGG 2 cut(s) 147, 1600
HhaI GCGC 2 cut(s) 101, 1794
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 8 cut(s) 106, 366, 535, 587, 891, 1576, 1694, 1894
Hin6I GCGC 2 cut(s) 99, 1792
HinP1I GCGC 2 cut(s) 99, 1792
HincII GTYRAC 1 cut(s) 1056
HindII GTYRAC 1 cut(s) 1056
HindIII AAGCTT 1 cut(s) 2141
HinfI GANTC 8 cut(s) 106, 259, 298, 332, 820, 835, 1157, 1840
HpaII CCGG 2 cut(s) 147, 1600
HphI GGTGA 6 cut(s) 37, 281, 935, 1484, 2008, 2072
Hpy166II GTNNAC 5 cut(s) 46, 144, 716, 1056, 1193
Hpy188III TCNNGA 9 cut(s) 241, 572, 584, 1295, 1394, 1496, 1844, 1907, 2135
Hpy8I GTNNAC 5 cut(s) 46, 144, 716, 1056, 1193
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 7 cut(s) 16, 211, 619, 721, 1016, 1032, 1930
HpyCH4III ACNGT 9 cut(s) 58, 89, 490, 560, 713, 811, 1327, 1933, 2044
HpyCH4IV ACGT 2 cut(s) 39, 2013
HpyF10VI GCNNNNNNNGC 5 cut(s) 160, 208, 1619, 1631, 1877
HpySE526I ACGT 2 cut(s) 39, 2013
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 8 cut(s) 106, 366, 535, 587, 891, 1576, 1694, 1894
HspAI GCGC 2 cut(s) 99, 1792
KasI GGCGCC 1 cut(s) 98
Ksp22I TGATCA 2 cut(s) 132, 925
LmnI GCTCC 2 cut(s) 1009, 1396
Lsp1109I GCAGC 8 cut(s) 52, 74, 373, 486, 809, 988, 994, 1609
LweI GCATC 3 cut(s) 874, 988, 1506
MaeI CTAG 7 cut(s) 1188, 1346, 1605, 1641, 1787, 1877, 2069
MaeII ACGT 2 cut(s) 39, 2013
MaeIII GTNAC 5 cut(s) 427, 449, 1454, 1919, 2044
MfeI CAATTG 1 cut(s) 1588
MflI RGATCY 5 cut(s) 177, 538, 1381, 1498, 1714
MhlI GDGCHC 4 cut(s) 718, 954, 1195, 1414
MlsI TGGCCA 1 cut(s) 2102
MluCI AATT 9 cut(s) 305, 567, 756, 801, 1044, 1426, 1588, 1969, 2029
MluNI TGGCCA 1 cut(s) 2102
Mly113I GGCGCC 1 cut(s) 99
MlyI GAGTC 3 cut(s) 341, 829, 1849
Mox20I TGGCCA 1 cut(s) 2102
Mph1103I ATGCAT 2 cut(s) 889, 1696
MroXI GAANNNNTTC 1 cut(s) 1262
MscI TGGCCA 1 cut(s) 2102
MseI TTAA 7 cut(s) 521, 860, 1403, 1539, 1809, 1836, 2018
MslI CAYNNNNRTG 5 cut(s) 828, 1656, 1723, 1895, 2091
Msp20I TGGCCA 1 cut(s) 2102
MspA1I CMGCKG 1 cut(s) 65
MspI CCGG 2 cut(s) 147, 1600
MspR9I CCNGG 6 cut(s) 735, 791, 840, 920, 1113, 1230
MunI CAATTG 1 cut(s) 1588
Mva1269I GAATGC 1 cut(s) 456
MvaI CCWGG 6 cut(s) 735, 791, 840, 920, 1113, 1230
MwoI GCNNNNNNNGC 5 cut(s) 160, 208, 1619, 1631, 1877
NarI GGCGCC 1 cut(s) 99
NdeI CATATG 1 cut(s) 825
NheI GCTAGC 1 cut(s) 1876
NlaIII CATG 8 cut(s) 106, 366, 535, 587, 891, 1576, 1694, 1894
NlaIV GGNNCC 7 cut(s) 100, 212, 732, 774, 951, 1383, 1500
NmuCI GTSAC 2 cut(s) 1919, 2044
NsbI TGCGCA 1 cut(s) 1793
NsiI ATGCAT 2 cut(s) 889, 1696
NspI RCATGY 2 cut(s) 366, 891
NspV TTCGAA 1 cut(s) 805
PaeI GCATGC 1 cut(s) 891
PaeR7I CTCGAG 1 cut(s) 1415
PagI TCATGA 1 cut(s) 583
PaqCI CACCTGC 1 cut(s) 1885
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PctI GAATGC 1 cut(s) 456
PdmI GAANNNNTTC 1 cut(s) 1262
PfeI GAWTC 5 cut(s) 106, 259, 298, 820, 1157
PfoI TCCNGGA 1 cut(s) 838
PkrI GCNGC 9 cut(s) 64, 67, 388, 476, 799, 893, 984, 1003, 1624
PleI GAGTC 3 cut(s) 340, 829, 1848
PluTI GGCGCC 1 cut(s) 102
PpsI GAGTC 3 cut(s) 340, 829, 1848
PshBI ATTAAT 1 cut(s) 1403
PsiI TTATAA 1 cut(s) 1137
Psp6I CCWGG 6 cut(s) 733, 789, 838, 918, 1111, 1228
PspFI CCCAGC 3 cut(s) 369, 1468, 1880
PspGI CCWGG 6 cut(s) 733, 789, 838, 918, 1111, 1228
PspN4I GGNNCC 7 cut(s) 100, 212, 732, 774, 951, 1383, 1500
PspPI GGNCC 3 cut(s) 144, 935, 1028
PspXI VCTCGAGB 1 cut(s) 1415
PstI CTGCAG 4 cut(s) 476, 1036, 1627, 1768
PstNI CAGNNNCTG 1 cut(s) 256
PsuI RGATCY 5 cut(s) 177, 538, 1381, 1498, 1714
PvuII CAGCTG 1 cut(s) 65
RseI CAYNNNNRTG 5 cut(s) 828, 1656, 1723, 1895, 2091
SaqAI TTAA 7 cut(s) 521, 860, 1403, 1539, 1809, 1836, 2018
SatI GCNGC 9 cut(s) 63, 66, 387, 475, 798, 892, 983, 1002, 1623
Sau96I GGNCC 3 cut(s) 144, 935, 1028
SbfI CCTGCAGG 1 cut(s) 1036
ScaI AGTACT 1 cut(s) 1828
SchI GAGTC 3 cut(s) 341, 829, 1849
ScrFI CCNGG 6 cut(s) 735, 791, 840, 920, 1113, 1230
SdaI CCTGCAGG 1 cut(s) 1036
SduI GDGCHC 4 cut(s) 718, 954, 1195, 1414
SfaNI GCATC 3 cut(s) 874, 988, 1506
SfcI CTRYAG 4 cut(s) 472, 1032, 1623, 1764
SfoI GGCGCC 1 cut(s) 100
Sfr274I CTCGAG 1 cut(s) 1415
SfuI TTCGAA 1 cut(s) 805
SinI GGWCC 3 cut(s) 144, 935, 1028
SlaI CTCGAG 1 cut(s) 1415
SmiMI CAYNNNNRTG 5 cut(s) 828, 1656, 1723, 1895, 2091
SmlI CTYRAG 6 cut(s) 182, 239, 680, 1415, 1814, 2135
SmoI CTYRAG 6 cut(s) 182, 239, 680, 1415, 1814, 2135
SphI GCATGC 1 cut(s) 891
Sse8387I CCTGCAGG 1 cut(s) 1036
Sse9I AATT 9 cut(s) 305, 567, 756, 801, 1044, 1426, 1588, 1969, 2029
SsiI CCGC 6 cut(s) 892, 1014, 1026, 1283, 2038, 2155
SspDI GGCGCC 1 cut(s) 98
SspI AATATT 1 cut(s) 993
SspMI CTAG 7 cut(s) 1188, 1346, 1605, 1641, 1787, 1877, 2069
StyD4I CCNGG 6 cut(s) 733, 789, 838, 918, 1111, 1228
StyI CCWWGG 1 cut(s) 1604
TaaI ACNGT 9 cut(s) 58, 89, 490, 560, 713, 811, 1327, 1933, 2044
TaiI ACGT 2 cut(s) 42, 2016
TaqI TCGA 6 cut(s) 36, 83, 805, 1416, 1562, 1656
TasI AATT 9 cut(s) 305, 567, 756, 801, 1044, 1426, 1588, 1969, 2029
TatI WGTACW 3 cut(s) 814, 1826, 1951
TauI GCSGC 1 cut(s) 894
TfiI GAWTC 5 cut(s) 106, 259, 298, 820, 1157
Tru1I TTAA 7 cut(s) 521, 860, 1403, 1539, 1809, 1836, 2018
Tru9I TTAA 7 cut(s) 521, 860, 1403, 1539, 1809, 1836, 2018
TseFI GTSAC 2 cut(s) 1919, 2044
TseI GCWGC 8 cut(s) 62, 65, 386, 474, 797, 982, 1001, 1622
Tsp45I GTSAC 2 cut(s) 1919, 2044
TspDTI ATGAA 9 cut(s) 119, 668, 734, 740, 812, 1355, 1644, 1725, 1879
TspGWI ACGGA 2 cut(s) 23, 2045
VneI GTGCAC 2 cut(s) 714, 1191
VpaK11BI GGWCC 3 cut(s) 144, 935, 1028
VspI ATTAAT 1 cut(s) 1403
XapI RAATTY 2 cut(s) 756, 2029
XceI RCATGY 2 cut(s) 366, 891
XhoI CTCGAG 1 cut(s) 1415
XmaJI CCTAGG 1 cut(s) 1604
XmnI GAANNNNTTC 1 cut(s) 1262
XspI CTAG 7 cut(s) 1188, 1346, 1605, 1641, 1787, 1877, 2069
ZrmI AGTACT 1 cut(s) 1828
Zsp2I ATGCAT 2 cut(s) 889, 1696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.