FvH4_4g10273

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001617
Physical Location & Seq
Forward (+)
13720837 .. 13722901
2065 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g10273.t1

Sequence Viewer

Length: 1164 bp
ATGAATGCTATCTGGAAGTTGAATATCCCTCCTAGAGCAAAAATTTTTGCTTGGCTCCTGGCCAGAGGGAGGCTGAAAACAAGGGCTAGATTAGCTCGTTACTCAAACACGATTACCACTTCTTGTACTCTCTGTAGCAATAGGGAGGAGGATTTAAACCACCTCTTCTTCTCCTGCCCCTTTTCTGTGAGTATTTGGAATATGGCTGGAAGGAAAAAGATAAAGAAACAGCTAAAAACTGCATTCTTCTTTGCTGGAAATTATGGGAGGCTCGAAACAATTATATTTTTAGAGGATGCACGCCTAACCCCAACTTTGATGTGCGCTGCTGCTTCTTTTGGTAATGCTTACAAAAGCCATACTCCGACTTCTAGAGGCCACAAGACCAACTCTAATGGATCCGCCATTCCAGCAAGTTCCACCATCAAATGGCTCCCGCCTCTGTGTAATTTTGTCAAGCTCAACTTTGATGGTTCCGTCTGCCACAACAAGAAGGCTGCTGCTGGGTCCCGCCCTCCGTGGAGACTTCGTACTCTTCTTAGCGATGTTGGCTGGATGGCTTCCAAATTTGAAGAGATCTCCTTCAAGTATGTCCCTTGGGAAGCAAATTTTGTGGCCGTTGCAATGGCTAGCTTGGGGTATCAGCCCAAGATATCAATGAAAGAGACGTCTGGAATGCTCCAGAGCGTCTATGTTCCGCGTTTGTTTTGTGACAGAAGTGAAAAGAAAGGCTGGGTCGAGGAAGCGGGATATGATTGGAGTGGGAACTGGTTTCTGGATCGGAACAGTCTGTTGGATTGGCTTGCCGGAGTCGGGCCGGATGGTGCAGATCGGAGGCGGGTTGGATCCAACAGCAAGCGAAGACGACGGATCCGAATCCAATTCAATGAGTTCTTTGTTTCGATTGGAGTTGGGTGGCTTGAGGTTTCTCGACGTTGGGTTGACAGTGACGGTGGCGCAAGGGTGGCTCGACTCTGGTCGGAAGGTGGCGACGATGGTGGGAAGACACAGCCTCTTCTTTGGGTCGGGCAGACTGCATGCCTGGGTTTGGACTTTTGGCCTTGGATACTGCATTTGGGTCCGATTTGGGCTGGTGATGGCGGTTGGGCCCACTTTTTGGGTCTGGCCCTGTTTGTTGTATTGTTTCCAGTTTTACTGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

388

Amino Acids

43.6

Weight (kDa)

9.89

Isoelectric Point (pI)

43.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 2 - 66 1.2e-18 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 671
AccB7I CCANNNNNTGG 2 cut(s) 429, 1117
AccII CGCG 1 cut(s) 700
AciI CCGC 7 cut(s) 402, 437, 511, 698, 746, 838, 1101
AclWI GGATC 7 cut(s) 393, 406, 786, 840, 853, 865, 878
AcoI YGGCCR 2 cut(s) 60, 615
AcsI RAATTY 3 cut(s) 42, 566, 607
AcyI GRCGYC 1 cut(s) 668
AfaI GTAC 2 cut(s) 127, 532
AfiI CCNNNNNNNGG 5 cut(s) 69, 429, 813, 1048, 1117
AgsI TTSAA 4 cut(s) 22, 572, 586, 886
AjnI CCWGG 2 cut(s) 57, 1041
AjuI GAANNNNNNNTTGG 2 cut(s) 776, 808
AluBI AGCT 4 cut(s) 95, 232, 460, 633
AluI AGCT 4 cut(s) 95, 232, 460, 633
Alw26I GTCTC 2 cut(s) 517, 659
AlwI GGATC 7 cut(s) 393, 406, 786, 840, 853, 865, 878
AoxI GGCC 7 cut(s) 60, 376, 615, 815, 1058, 1107, 1125
ApaI GGGCCC 1 cut(s) 1111
ApeKI GCWGC 4 cut(s) 326, 329, 497, 500
ApoI RAATTY 3 cut(s) 42, 566, 607
AspLEI GCGC 2 cut(s) 326, 959
AspS9I GGNCC 6 cut(s) 507, 815, 1079, 1107, 1108, 1126
AsuHPI GGTGA 1 cut(s) 1106
AsuNHI GCTAGC 1 cut(s) 629
AvaII GGWCC 2 cut(s) 507, 1079
BaeGI GKGCMC 1 cut(s) 1111
BalI TGGCCA 1 cut(s) 62
BamHI GGATCC 3 cut(s) 398, 845, 870
BanII GRGCYC 1 cut(s) 1111
BbsI GAAGAC 2 cut(s) 868, 1010
BbvI GCAGC 4 cut(s) 313, 316, 484, 487
BccI CCATC 6 cut(s) 431, 464, 550, 815, 989, 1091
BceAI ACGGC 1 cut(s) 602
BciT130I CCWGG 2 cut(s) 59, 1043
BciVI GTATCC 1 cut(s) 1059
BcoDI GTCTC 2 cut(s) 517, 659
BfaI CTAG 4 cut(s) 33, 87, 372, 630
BfmI CTRYAG 1 cut(s) 133
BfuI GTATCC 1 cut(s) 1059
BglII AGATCT 1 cut(s) 576
BisI GCNGC 4 cut(s) 327, 330, 498, 501
BlsI GCNGC 4 cut(s) 328, 331, 499, 502
Bme1390I CCNGG 2 cut(s) 59, 1043
Bme18I GGWCC 2 cut(s) 507, 1079
BmgT120I GGNCC 6 cut(s) 507, 815, 1079, 1107, 1108, 1126
BmrFI CCNGG 2 cut(s) 59, 1043
BmsI GCATC 1 cut(s) 286
BmtI GCTAGC 1 cut(s) 633
BoxI GACNNNNGTC 1 cut(s) 976
BpiI GAAGAC 2 cut(s) 868, 1010
BpmI CTGGAG 1 cut(s) 665
BpuEI CTTGAG 1 cut(s) 941
BsaBI GATNNNNATC 1 cut(s) 875
BsaHI GRCGYC 1 cut(s) 668
BsaJI CCNNGG 4 cut(s) 518, 596, 1042, 1061
BsaXI ACNNNNNCTCC 2 cut(s) 514, 544
Bsc4I CCNNNNNNNGG 5 cut(s) 69, 429, 813, 1048, 1117
Bse1I ACTGG 2 cut(s) 773, 1148
Bse3DI GCAATG 1 cut(s) 630
Bse8I GATNNNNATC 1 cut(s) 875
BseBI CCWGG 2 cut(s) 59, 1043
BseDI CCNNGG 4 cut(s) 518, 596, 1042, 1061
BseGI GGATG 3 cut(s) 301, 561, 826
BseJI GATNNNNATC 1 cut(s) 875
BseLI CCNNNNNNNGG 5 cut(s) 69, 429, 813, 1048, 1117
BseMI GCAATG 1 cut(s) 630
BseNI ACTGG 2 cut(s) 773, 1148
BseRI GAGGAG 1 cut(s) 161
BseSI GKGCMC 1 cut(s) 1111
BseXI GCAGC 4 cut(s) 313, 316, 484, 487
BseYI CCCAGC 2 cut(s) 503, 732
BsgI GTGCAG 1 cut(s) 846
Bsh1236I CGCG 1 cut(s) 700
BshFI GGCC 7 cut(s) 62, 378, 617, 817, 1060, 1109, 1127
BsiSI CCGG 2 cut(s) 807, 818
BslFI GGGAC 2 cut(s) 493, 578
BslI CCNNNNNNNGG 5 cut(s) 69, 429, 813, 1048, 1117
BsmAI GTCTC 2 cut(s) 517, 659
BsmBI CGTCTC 1 cut(s) 659
BsmFI GGGAC 2 cut(s) 493, 578
BsmI GAATGC 3 cut(s) 10, 242, 681
BsnI GGCC 7 cut(s) 62, 378, 617, 817, 1060, 1109, 1127
Bsp120I GGGCCC 1 cut(s) 1107
Bsp1286I GDGCHC 1 cut(s) 1111
Bsp143I GATC 6 cut(s) 398, 576, 778, 829, 845, 870
BspACI CCGC 7 cut(s) 402, 437, 511, 698, 746, 838, 1101
BspANI GGCC 7 cut(s) 62, 378, 617, 817, 1060, 1109, 1127
BspFNI CGCG 1 cut(s) 700
BspOI GCTAGC 1 cut(s) 633
BspPI GGATC 7 cut(s) 393, 406, 786, 840, 853, 865, 878
BsrDI GCAATG 1 cut(s) 630
BsrI ACTGG 2 cut(s) 773, 1148
BssECI CCNNGG 4 cut(s) 518, 596, 1042, 1061
BssMI GATC 6 cut(s) 398, 576, 778, 829, 845, 870
BssNI GRCGYC 1 cut(s) 668
BssT1I CCWWGG 2 cut(s) 596, 1061
Bst2UI CCWGG 2 cut(s) 59, 1043
Bst4CI ACNGT 4 cut(s) 788, 947, 953, 1158
Bst6I CTCTTC 4 cut(s) 170, 540, 567, 1020
BstACI GRCGYC 1 cut(s) 668
BstC8I GCNNGC 5 cut(s) 301, 631, 804, 857, 1039
BstDEI CTNAG 1 cut(s) 539
BstDSI CCRYGG 1 cut(s) 518
BstF5I GGATG 3 cut(s) 301, 561, 826
BstFNI CGCG 1 cut(s) 700
BstHHI GCGC 2 cut(s) 326, 959
BstKTI GATC 6 cut(s) 401, 579, 781, 832, 848, 873
BstMAI GTCTC 2 cut(s) 517, 659
BstMBI GATC 6 cut(s) 398, 576, 778, 829, 845, 870
BstMWI GCNNNNNNNGC 4 cut(s) 92, 410, 549, 965
BstNI CCWGG 2 cut(s) 59, 1043
BstNSI RCATGY 1 cut(s) 1041
BstPAI GACNNNNGTC 1 cut(s) 976
BstSCI CCNGG 2 cut(s) 57, 1041
BstSFI CTRYAG 1 cut(s) 133
BstSLI GKGCMC 1 cut(s) 1111
BstUI CGCG 1 cut(s) 700
BstV1I GCAGC 4 cut(s) 313, 316, 484, 487
BstV2I GAAGAC 2 cut(s) 868, 1010
BstX2I RGATCY 4 cut(s) 398, 576, 845, 870
BstYI RGATCY 4 cut(s) 398, 576, 845, 870
BsuI GTATCC 1 cut(s) 1059
BsuRI GGCC 7 cut(s) 62, 378, 617, 817, 1060, 1109, 1127
BtgI CCRYGG 1 cut(s) 518
BtgZI GCGATG 1 cut(s) 558
BtsCI GGATG 3 cut(s) 301, 561, 826
BtsIMutI CAGTG 1 cut(s) 952
Cac8I GCNNGC 5 cut(s) 301, 631, 804, 857, 1039
CfoI GCGC 2 cut(s) 326, 959
Cfr13I GGNCC 6 cut(s) 507, 815, 1079, 1107, 1108, 1126
CseI GACGC 1 cut(s) 676
Csp6I GTAC 2 cut(s) 126, 531
CspCI CAANNNNNGTGG 2 cut(s) 594, 629
CviAII CATG 1 cut(s) 1038
CviQI GTAC 2 cut(s) 126, 531
DdeI CTNAG 1 cut(s) 539
DpnI GATC 6 cut(s) 400, 578, 780, 831, 847, 872
DpnII GATC 6 cut(s) 398, 576, 778, 829, 845, 870
DraI TTTAAA 1 cut(s) 156
EaeI YGGCCR 2 cut(s) 60, 615
Eam1104I CTCTTC 4 cut(s) 170, 540, 567, 1020
EarI CTCTTC 4 cut(s) 170, 540, 567, 1020
EciI GGCGGA 1 cut(s) 391
Eco130I CCWWGG 2 cut(s) 596, 1061
Eco24I GRGCYC 1 cut(s) 1111
Eco32I GATATC 1 cut(s) 654
Eco47I GGWCC 2 cut(s) 507, 1079
EcoO109I RGGNCCY 1 cut(s) 507
EcoRII CCWGG 2 cut(s) 57, 1041
EcoRV GATATC 1 cut(s) 654
EcoT14I CCWWGG 2 cut(s) 596, 1061
EcoT38I GRGCYC 1 cut(s) 1111
ErhI CCWWGG 2 cut(s) 596, 1061
Esp3I CGTCTC 1 cut(s) 659
FaeI CATG 1 cut(s) 1041
FaiI YATR 8 cut(s) 203, 264, 284, 360, 591, 693, 753, 1039
FalI AAGNNNNNCTT 2 cut(s) 449, 481
FaqI GGGAC 2 cut(s) 493, 578
FatI CATG 1 cut(s) 1037
FauI CCCGC 4 cut(s) 444, 518, 739, 831
Fnu4HI GCNGC 4 cut(s) 327, 330, 498, 501
FokI GGATG 3 cut(s) 308, 568, 833
FriOI GRGCYC 1 cut(s) 1111
Fsp4HI GCNGC 4 cut(s) 327, 330, 498, 501
FspBI CTAG 4 cut(s) 33, 87, 372, 630
GlaI GCGC 2 cut(s) 325, 958
GluI GCNGC 4 cut(s) 327, 330, 498, 501
GsaI CCCAGC 2 cut(s) 507, 736
GsuI CTGGAG 1 cut(s) 665
HaeIII GGCC 7 cut(s) 62, 378, 617, 817, 1060, 1109, 1127
HapII CCGG 2 cut(s) 807, 818
HgaI GACGC 1 cut(s) 676
HhaI GCGC 2 cut(s) 326, 959
Hin1I GRCGYC 1 cut(s) 668
Hin1II CATG 1 cut(s) 1041
Hin6I GCGC 2 cut(s) 324, 957
HinP1I GCGC 2 cut(s) 324, 957
HincII GTYRAC 1 cut(s) 943
HindII GTYRAC 1 cut(s) 943
HinfI GANTC 3 cut(s) 810, 876, 972
HpaII CCGG 2 cut(s) 807, 818
HphI GGTGA 1 cut(s) 1106
Hpy166II GTNNAC 1 cut(s) 943
Hpy188I TCNGA 6 cut(s) 366, 783, 834, 875, 982, 1083
Hpy188III TCNNGA 6 cut(s) 13, 372, 672, 682, 776, 930
Hpy8I GTNNAC 1 cut(s) 943
Hpy99I CGWCG 3 cut(s) 870, 936, 995
HpyAV CCTTC 4 cut(s) 204, 487, 592, 977
HpyCH4III ACNGT 4 cut(s) 788, 947, 953, 1158
HpyCH4IV ACGT 2 cut(s) 668, 934
HpyCH4V TGCA 6 cut(s) 242, 299, 623, 827, 1037, 1072
HpyF10VI GCNNNNNNNGC 4 cut(s) 92, 410, 549, 965
HpyF3I CTNAG 1 cut(s) 539
HpySE526I ACGT 2 cut(s) 668, 934
Hsp92I GRCGYC 1 cut(s) 668
Hsp92II CATG 1 cut(s) 1041
HspAI GCGC 2 cut(s) 324, 957
KflI GGGWCCC 1 cut(s) 507
Kzo9I GATC 6 cut(s) 398, 576, 778, 829, 845, 870
LmnI GCTCC 3 cut(s) 60, 438, 684
Lsp1109I GCAGC 4 cut(s) 313, 316, 484, 487
LweI GCATC 1 cut(s) 286
MaeI CTAG 4 cut(s) 33, 87, 372, 630
MaeII ACGT 2 cut(s) 668, 934
MaeIII GTNAC 3 cut(s) 98, 710, 947
MalI GATC 6 cut(s) 400, 578, 780, 831, 847, 872
MboI GATC 6 cut(s) 398, 576, 778, 829, 845, 870
MboII GAAGA 8 cut(s) 157, 160, 238, 527, 584, 873, 1007, 1015
MflI RGATCY 4 cut(s) 398, 576, 845, 870
MhlI GDGCHC 1 cut(s) 1111
MlsI TGGCCA 1 cut(s) 62
MluCI AATT 7 cut(s) 42, 259, 279, 448, 566, 607, 881
MluNI TGGCCA 1 cut(s) 62
MlyI GAGTC 2 cut(s) 819, 966
MmeI TCCRAC 5 cut(s) 389, 774, 823, 873, 960
Mox20I TGGCCA 1 cut(s) 62
MscI TGGCCA 1 cut(s) 62
MseI TTAA 1 cut(s) 155
Msp20I TGGCCA 1 cut(s) 62
MspI CCGG 2 cut(s) 807, 818
MspR9I CCNGG 2 cut(s) 59, 1043
Mva1269I GAATGC 3 cut(s) 10, 242, 681
MvaI CCWGG 2 cut(s) 59, 1043
MvnI CGCG 1 cut(s) 700
MwoI GCNNNNNNNGC 4 cut(s) 92, 410, 549, 965
NdeII GATC 6 cut(s) 398, 576, 778, 829, 845, 870
NheI GCTAGC 1 cut(s) 629
NlaIII CATG 1 cut(s) 1041
NmuCI GTSAC 2 cut(s) 710, 947
NspI RCATGY 1 cut(s) 1041
PaeI GCATGC 1 cut(s) 1041
PcsI WCGNNNNNNNCGW 1 cut(s) 871
PctI GAATGC 3 cut(s) 10, 242, 681
PfeI GAWTC 1 cut(s) 876
PflMI CCANNNNNTGG 2 cut(s) 429, 1117
PkrI GCNGC 4 cut(s) 328, 331, 499, 502
PleI GAGTC 2 cut(s) 818, 966
PpsI GAGTC 2 cut(s) 818, 966
PpuMI RGGWCCY 1 cut(s) 507
PshAI GACNNNNGTC 1 cut(s) 976
Psp5II RGGWCCY 1 cut(s) 507
Psp6I CCWGG 2 cut(s) 57, 1041
PspFI CCCAGC 2 cut(s) 503, 732
PspGI CCWGG 2 cut(s) 57, 1041
PspOMI GGGCCC 1 cut(s) 1107
PspPI GGNCC 6 cut(s) 507, 815, 1079, 1107, 1108, 1126
PspPPI RGGWCCY 1 cut(s) 507
PsuI RGATCY 4 cut(s) 398, 576, 845, 870
RsaI GTAC 2 cut(s) 127, 532
RsaNI GTAC 2 cut(s) 126, 531
SaqAI TTAA 1 cut(s) 155
SatI GCNGC 4 cut(s) 327, 330, 498, 501
Sau3AI GATC 6 cut(s) 398, 576, 778, 829, 845, 870
Sau96I GGNCC 6 cut(s) 507, 815, 1079, 1107, 1108, 1126
SchI GAGTC 2 cut(s) 819, 966
ScrFI CCNGG 2 cut(s) 59, 1043
SduI GDGCHC 1 cut(s) 1111
SetI ASST 9 cut(s) 97, 165, 234, 462, 635, 671, 927, 937, 988
SfaNI GCATC 1 cut(s) 286
SfcI CTRYAG 1 cut(s) 133
SinI GGWCC 2 cut(s) 507, 1079
SmlI CTYRAG 1 cut(s) 920
SmoI CTYRAG 1 cut(s) 920
SphI GCATGC 1 cut(s) 1041
Sse9I AATT 7 cut(s) 42, 259, 279, 448, 566, 607, 881
SsiI CCGC 7 cut(s) 402, 437, 511, 698, 746, 838, 1101
SspMI CTAG 4 cut(s) 33, 87, 372, 630
StyD4I CCNGG 2 cut(s) 57, 1041
StyI CCWWGG 2 cut(s) 596, 1061
TaaI ACNGT 4 cut(s) 788, 947, 953, 1158
TaiI ACGT 2 cut(s) 671, 937
TaqI TCGA 5 cut(s) 273, 738, 902, 931, 970
TasI AATT 7 cut(s) 42, 259, 279, 448, 566, 607, 881
TatI WGTACW 1 cut(s) 125
TfiI GAWTC 1 cut(s) 876
Tru1I TTAA 1 cut(s) 155
Tru9I TTAA 1 cut(s) 155
TscAI CASTG 1 cut(s) 952
TseFI GTSAC 2 cut(s) 710, 947
TseI GCWGC 4 cut(s) 326, 329, 497, 500
Tsp45I GTSAC 2 cut(s) 710, 947
TspDTI ATGAA 2 cut(s) 17, 674
TspGWI ACGGA 3 cut(s) 466, 507, 883
TspRI CASTG 1 cut(s) 952
Van91I CCANNNNNTGG 2 cut(s) 429, 1117
VpaK11BI GGWCC 2 cut(s) 507, 1079
XapI RAATTY 3 cut(s) 42, 566, 607
XbaI TCTAGA 1 cut(s) 371
XceI RCATGY 1 cut(s) 1041
XspI CTAG 4 cut(s) 33, 87, 372, 630
ZraI GACGTC 1 cut(s) 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.