FvH4_2g33151

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001095
Physical Location & Seq
Reverse (-)
24927936 .. 24931851
3916 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33151.t1

Sequence Viewer

Length: 1821 bp
ATGTCAGAACCTCCTGATCTAGGAAATTGGTTCGCTAGCTACCGGTATGCATCACCTGCAATGAATTCCAATTCTTTTCACAATGAGTTTGGTACCGGCGAGAGTGAAAGAGGAAAAGATGAAGGGAATTCATGTGGGTTTAGGGAAATTGGAAATGAAGTTGGAGTCTTTGTTCATGAGAAGGCAGATGGGGTTGTCAAATGTGGCAGGTCTTCACAAGACCAATGTTTAAGTAAGATTTCAGGTTCTTCGGAATCACCTTTGTCCCTTTCAGAGCCTACGGACATCAAAAACTGGTTTTCAAGCTATGTGTACGAGTCTCCTTCATTGGATACTACTGATGGTTTTGGAGATTCTCTTGCCAAGCAAAGTAAATGCGAGAAGGATGGGTTTTTGGTTGAAAACAGTAAGAGAGAAAATGAAGAGGAATTGAGGAATTCTAGTATAAAGAGGAGTATCAGTAGAGAGGTTGTGGGTGAGAAGGAACAGTCAGGAGGGCTTTCGAATTGTGACAGCTCTTGGAGAGTTACTAAGCAGGCTCCAGGTCCTCTGGAATCACCTTTGCTTCTTTCAGAACCTGTTCACATCAAAAACTGGTTTTCAAGCTATGTGTATGAGTCTCCTTCCTTGGATACATATGATAATGATTCTGTTGGCAAAGAAAGTAAATGTGAGAAGGATGGCTTTATGGTTGAAAATAGAAAGAGTAATATTGAAGAGAATTTGGGGGATTTTAGAAAGAGTAGTGTTGGTAAAGAGGTTGTGGATGAGAAGAAACAATTGGTAGGGCTTGCAAAATTTGACAGCTCTTGGAGAGACACCAAGCATGATAGGCAGCCTGTACATGAGGTTCCAGGTCATTTGGACTCCTCTTCACACTTGTCAGAGCCTACTGACATCCAGACTTGGTTCTCAAGCTATGTGTATGAGTCTCCATCTTTGGATACAGCTGATGGTTTTGGAGATGTTGTTTGTAGAGATAGAATAGATGAGAAGGATGAGGTTTCGGTTGAAACCAGCAACAAAGAAAAGGGAGAGCGTTTGGAGAGGTTTGGTAAAAAAGAAAGTGATAATGAAGAGTTTGATGTTGAGAAGGTGCAGTCAGAAGAGTTAGCAAAACATAACACCACTTCGAGACATAGCAAGAAGCCTGTTGACCAAACCACTGGACTGGATGAAAATCTATCTGGTCAGAGTGATTTTTGTCTCAAATATAAGCCTAATATGTTGCAAAATTATCGTAGTAGTCAACCAAAACATGCTGAGACATCAAGCTTCAACAAAGGAGATTCTCAATGCAAGGTAGATCAACAGACCTTCCATGGAACCAGATCTAAGCCATTGAACAGGAGAAGTTCATGCAGTAATGATGGGACATTGGTTCATAGTAGGGGCCCCACTTCACAGGAGAGCTCAGAAGCAAAGGTCCATGTTGAAGAATTCTTACTAGAAACCAATTCCGAGTTAATTTCAGTCAATGAAGCATCAGTAAGCAAAACAACTCATGGAAGCAAAGAAAGAAAAGATGATGGGACAGGAATATCAGAAGATGGTTTCATTACAACAAGGAAACGCAAATTTACAGCAAGAAATGATGAAAACTCTCCAGAAGGACCACCAAGGAGTAGTAGAGGATCAGTTCCATCAGTACGTGGGAAAGATGTTGTTATTCAAAGAAAGACCTTGGCAGACACAACAAACTTTCATCATTCTCCAGCAACAATGATCACCGGAAAATGGAAGTGTCCTCAGCAGCGTAAGCCAAATTTGGGACCTCCTTTGAAGCAGCTTGGACTTGAGCACTGGATTCGAAAGTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

607

Amino Acids

67.72

Weight (kDa)

5.65

Isoelectric Point (pI)

52.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 64
Acc36I ACCTGC 2 cut(s) 64, 198
Acc65I GGTACC 1 cut(s) 92
AccB1I GGYRCC 1 cut(s) 92
AclWI GGATC 1 cut(s) 1642
AcsI RAATTY 8 cut(s) 64, 127, 436, 721, 797, 1439, 1577, 1765
AfaI GTAC 4 cut(s) 94, 314, 843, 1650
AfiI CCNNNNNNNGG 3 cut(s) 20, 1737, 1769
AgeI ACCGGT 1 cut(s) 42
AjnI CCWGG 2 cut(s) 541, 853
AjuI GAANNNNNNNTTGG 2 cut(s) 764, 796
AloI GAACNNNNNNTCC 2 cut(s) 156, 188
Alw21I GWGCWC 2 cut(s) 1415, 1803
Alw26I GTCTC 7 cut(s) 324, 624, 810, 936, 1129, 1211, 1259
AlwI GGATC 1 cut(s) 1642
AlwNI CAGNNNCTG 1 cut(s) 578
AoxI GGCC 1 cut(s) 1393
ApaI GGGCCC 1 cut(s) 1397
ApeKI GCWGC 3 cut(s) 835, 1753, 1786
ApoI RAATTY 8 cut(s) 64, 127, 436, 721, 797, 1439, 1577, 1765
AsiGI ACCGGT 1 cut(s) 42
Asp700I GAANNNNTTC 1 cut(s) 579
Asp718I GGTACC 1 cut(s) 92
AspS9I GGNCC 6 cut(s) 545, 1393, 1394, 1426, 1613, 1772
AsuHPI GGTGA 5 cut(s) 45, 249, 488, 549, 1720
AsuII TTCGAA 2 cut(s) 503, 1810
AsuNHI GCTAGC 1 cut(s) 35
AvaII GGWCC 4 cut(s) 545, 1426, 1613, 1772
BaeGI GKGCMC 1 cut(s) 1397
BanI GGYRCC 1 cut(s) 92
BanII GRGCYC 2 cut(s) 1397, 1415
BarI GAAGNNNNNNTAC 2 cut(s) 1428, 1460
BbsI GAAGAC 1 cut(s) 204
Bbv12I GWGCWC 2 cut(s) 1415, 1803
BbvCI CCTCAGC 1 cut(s) 1749
BbvI GCAGC 3 cut(s) 847, 1765, 1798
BcgI CGANNNNNNTGC 3 cut(s) 1220, 1254, 1790
BciT130I CCWGG 2 cut(s) 543, 855
BciVI GTATCC 3 cut(s) 325, 625, 937
BclI TGATCA 1 cut(s) 1725
BcoDI GTCTC 7 cut(s) 324, 624, 810, 936, 1129, 1211, 1259
BfaI CTAG 4 cut(s) 20, 36, 441, 1448
BfuAI ACCTGC 2 cut(s) 64, 198
BfuI GTATCC 3 cut(s) 325, 625, 937
BglII AGATCT 1 cut(s) 1331
BisI GCNGC 3 cut(s) 836, 1754, 1787
BlsI GCNGC 3 cut(s) 837, 1755, 1788
Bme1390I CCNGG 2 cut(s) 543, 855
Bme18I GGWCC 4 cut(s) 545, 1426, 1613, 1772
BmgT120I GGNCC 6 cut(s) 545, 1393, 1394, 1426, 1613, 1772
BmiI GGNNCC 8 cut(s) 94, 540, 852, 1327, 1394, 1395, 1396, 1773
BmrFI CCNGG 2 cut(s) 543, 855
BmsI GCATC 2 cut(s) 59, 1493
BmtI GCTAGC 1 cut(s) 39
BpiI GAAGAC 1 cut(s) 204
BpmI CTGGAG 3 cut(s) 525, 1590, 1698
Bpu10I CCTNAGC 1 cut(s) 1749
Bpu14I TTCGAA 2 cut(s) 503, 1810
BpuEI CTTGAG 2 cut(s) 898, 1817
BsaAI YACGTR 1 cut(s) 1652
BsaBI GATNNNNATC 1 cut(s) 1179
BsaJI CCNNGG 4 cut(s) 627, 1321, 1619, 1683
BsaWI WCCGGW 2 cut(s) 42, 1730
BsaXI ACNNNNNCTCC 6 cut(s) 156, 186, 445, 475, 954, 984
Bsc4I CCNNNNNNNGG 3 cut(s) 20, 1737, 1769
Bse118I RCCGGY 2 cut(s) 42, 95
Bse1I ACTGG 5 cut(s) 299, 599, 1171, 1176, 1808
Bse3DI GCAATG 1 cut(s) 66
Bse8I GATNNNNATC 1 cut(s) 1179
BseBI CCWGG 2 cut(s) 543, 855
BseDI CCNNGG 4 cut(s) 627, 1321, 1619, 1683
BseGI GGATG 6 cut(s) 391, 685, 772, 897, 1003, 1180
BseJI GATNNNNATC 1 cut(s) 1179
BseLI CCNNNNNNNGG 3 cut(s) 20, 1737, 1769
BseMI GCAATG 1 cut(s) 66
BseMII CTCAG 3 cut(s) 1254, 1428, 1763
BseNI ACTGG 5 cut(s) 299, 599, 1171, 1176, 1808
BseRI GAGGAG 2 cut(s) 466, 859
BseSI GKGCMC 1 cut(s) 1397
BseXI GCAGC 3 cut(s) 847, 1765, 1798
BsgI GTGCAG 1 cut(s) 1118
BshFI GGCC 1 cut(s) 1395
BshNI GGYRCC 1 cut(s) 92
BshTI ACCGGT 1 cut(s) 42
BsiHKAI GWGCWC 2 cut(s) 1415, 1803
BsiSI CCGG 3 cut(s) 43, 96, 1731
BslFI GGGAC 4 cut(s) 250, 1387, 1546, 1785
BslI CCNNNNNNNGG 3 cut(s) 20, 1737, 1769
BsmAI GTCTC 7 cut(s) 324, 624, 810, 936, 1129, 1211, 1259
BsmFI GGGAC 4 cut(s) 250, 1387, 1546, 1785
BsnI GGCC 1 cut(s) 1395
Bsp119I TTCGAA 2 cut(s) 503, 1810
Bsp120I GGGCCC 1 cut(s) 1393
Bsp1286I GDGCHC 3 cut(s) 1397, 1415, 1803
Bsp1407I TGTACA 1 cut(s) 841
Bsp143I GATC 5 cut(s) 16, 1306, 1331, 1634, 1725
Bsp19I CCATGG 1 cut(s) 1321
BspANI GGCC 1 cut(s) 1395
BspCNI CTCAG 3 cut(s) 1255, 1427, 1762
BspHI TCATGA 1 cut(s) 175
BspLI GGNNCC 8 cut(s) 94, 540, 852, 1327, 1394, 1395, 1396, 1773
BspMI ACCTGC 2 cut(s) 64, 198
BspOI GCTAGC 1 cut(s) 39
BspPI GGATC 1 cut(s) 1642
BspT104I TTCGAA 2 cut(s) 503, 1810
BspT107I GGYRCC 1 cut(s) 92
BsrDI GCAATG 1 cut(s) 66
BsrFI RCCGGY 2 cut(s) 42, 95
BsrGI TGTACA 1 cut(s) 841
BsrI ACTGG 5 cut(s) 299, 599, 1171, 1176, 1808
BssAI RCCGGY 2 cut(s) 42, 95
BssECI CCNNGG 4 cut(s) 627, 1321, 1619, 1683
BssMI GATC 5 cut(s) 16, 1306, 1331, 1634, 1725
BssT1I CCWWGG 4 cut(s) 627, 1321, 1619, 1683
Bst2UI CCWGG 2 cut(s) 543, 855
Bst4CI ACNGT 2 cut(s) 407, 489
Bst6I CTCTTC 5 cut(s) 417, 711, 877, 1071, 1101
BstAPI GCANNNNNTGC 1 cut(s) 56
BstAUI TGTACA 1 cut(s) 841
BstBAI YACGTR 1 cut(s) 1652
BstBI TTCGAA 2 cut(s) 503, 1810
BstC8I GCNNGC 3 cut(s) 37, 537, 792
BstDEI CTNAG 5 cut(s) 531, 1263, 1335, 1414, 1749
BstDSI CCRYGG 1 cut(s) 1321
BstENI CCTNNNNNAGG 1 cut(s) 18
BstF5I GGATG 6 cut(s) 391, 685, 772, 897, 1003, 1180
BstKTI GATC 5 cut(s) 19, 1309, 1334, 1637, 1728
BstMAI GTCTC 7 cut(s) 324, 624, 810, 936, 1129, 1211, 1259
BstMBI GATC 5 cut(s) 16, 1306, 1331, 1634, 1725
BstMWI GCNNNNNNNGC 3 cut(s) 56, 832, 1759
BstNI CCWGG 2 cut(s) 543, 855
BstNSI RCATGY 1 cut(s) 1262
BstSCI CCNGG 2 cut(s) 541, 853
BstSLI GKGCMC 1 cut(s) 1397
BstV1I GCAGC 3 cut(s) 847, 1765, 1798
BstV2I GAAGAC 1 cut(s) 204
BstX2I RGATCY 1 cut(s) 1331
BstXI CCANNNNNNTGG 2 cut(s) 1166, 1171
BstYI RGATCY 1 cut(s) 1331
BsuI GTATCC 3 cut(s) 325, 625, 937
BsuRI GGCC 1 cut(s) 1395
BtgI CCRYGG 1 cut(s) 1321
BtsCI GGATG 6 cut(s) 391, 685, 772, 897, 1003, 1180
BtsIMutI CAGTG 2 cut(s) 1164, 1801
BveI ACCTGC 2 cut(s) 64, 198
Cac8I GCNNGC 3 cut(s) 37, 537, 792
CaiI CAGNNNCTG 1 cut(s) 578
CciI TCATGA 1 cut(s) 175
Cfr10I RCCGGY 2 cut(s) 42, 95
Cfr13I GGNCC 6 cut(s) 545, 1393, 1394, 1426, 1613, 1772
Csp6I GTAC 4 cut(s) 93, 313, 842, 1649
CspAI ACCGGT 1 cut(s) 42
CviAII CATG 9 cut(s) 132, 176, 827, 845, 1259, 1322, 1359, 1430, 1505
CviQI GTAC 4 cut(s) 93, 313, 842, 1649
DdeI CTNAG 5 cut(s) 531, 1263, 1335, 1414, 1749
DpnI GATC 5 cut(s) 18, 1308, 1333, 1636, 1727
DpnII GATC 5 cut(s) 16, 1306, 1331, 1634, 1725
Eam1104I CTCTTC 5 cut(s) 417, 711, 877, 1071, 1101
EarI CTCTTC 5 cut(s) 417, 711, 877, 1071, 1101
Ecl136II GAGCTC 1 cut(s) 1413
Eco130I CCWWGG 4 cut(s) 627, 1321, 1619, 1683
Eco24I GRGCYC 2 cut(s) 1397, 1415
Eco47I GGWCC 4 cut(s) 545, 1426, 1613, 1772
Eco53kI GAGCTC 1 cut(s) 1413
EcoICRI GAGCTC 1 cut(s) 1413
EcoNI CCTNNNNNAGG 1 cut(s) 18
EcoO109I RGGNCCY 4 cut(s) 545, 1393, 1394, 1772
EcoRI GAATTC 4 cut(s) 64, 127, 436, 1439
EcoRII CCWGG 2 cut(s) 541, 853
EcoT14I CCWWGG 4 cut(s) 627, 1321, 1619, 1683
EcoT22I ATGCAT 1 cut(s) 52
EcoT38I GRGCYC 2 cut(s) 1397, 1415
ErhI CCWWGG 4 cut(s) 627, 1321, 1619, 1683
FaeI CATG 9 cut(s) 135, 179, 830, 848, 1262, 1325, 1362, 1433, 1508
FalI AAGNNNNNCTT 2 cut(s) 668, 700
FaqI GGGAC 4 cut(s) 250, 1387, 1546, 1785
FatI CATG 9 cut(s) 131, 175, 826, 844, 1258, 1321, 1358, 1429, 1504
FauNDI CATATG 1 cut(s) 637
FbaI TGATCA 1 cut(s) 1725
Fnu4HI GCNGC 3 cut(s) 836, 1754, 1787
FokI GGATG 6 cut(s) 398, 692, 779, 884, 1010, 1187
FriOI GRGCYC 2 cut(s) 1397, 1415
Fsp4HI GCNGC 3 cut(s) 836, 1754, 1787
FspBI CTAG 4 cut(s) 20, 36, 441, 1448
GluI GCNGC 3 cut(s) 836, 1754, 1787
GsuI CTGGAG 3 cut(s) 525, 1590, 1698
HaeIII GGCC 1 cut(s) 1395
HapII CCGG 3 cut(s) 43, 96, 1731
Hin1II CATG 9 cut(s) 135, 179, 830, 848, 1262, 1325, 1362, 1433, 1508
HincII GTYRAC 2 cut(s) 1156, 1250
HindII GTYRAC 2 cut(s) 1156, 1250
HindIII AAGCTT 1 cut(s) 1273
HpaII CCGG 3 cut(s) 43, 96, 1731
HphI GGTGA 5 cut(s) 45, 249, 488, 549, 1720
Hpy166II GTNNAC 4 cut(s) 313, 583, 1156, 1250
Hpy188III TCNNGA 8 cut(s) 14, 176, 492, 551, 901, 1134, 1607, 1818
Hpy8I GTNNAC 4 cut(s) 313, 583, 1156, 1250
HpyCH4III ACNGT 2 cut(s) 407, 489
HpyCH4IV ACGT 1 cut(s) 1651
HpyCH4V TGCA 7 cut(s) 50, 59, 794, 1099, 1231, 1299, 1362
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 832, 1759
HpyF3I CTNAG 5 cut(s) 531, 1263, 1335, 1414, 1749
HpySE526I ACGT 1 cut(s) 1651
Hsp92II CATG 9 cut(s) 135, 179, 830, 848, 1262, 1325, 1362, 1433, 1508
KpnI GGTACC 1 cut(s) 96
Ksp22I TGATCA 1 cut(s) 1725
Kzo9I GATC 5 cut(s) 16, 1306, 1331, 1634, 1725
LmnI GCTCC 1 cut(s) 544
Lsp1109I GCAGC 3 cut(s) 847, 1765, 1798
LweI GCATC 2 cut(s) 59, 1493
MaeI CTAG 4 cut(s) 20, 36, 441, 1448
MaeII ACGT 1 cut(s) 1651
MaeIII GTNAC 2 cut(s) 509, 526
MalI GATC 5 cut(s) 18, 1308, 1333, 1636, 1727
MboI GATC 5 cut(s) 16, 1306, 1331, 1634, 1725
MfeI CAATTG 1 cut(s) 779
MflI RGATCY 1 cut(s) 1331
MhlI GDGCHC 3 cut(s) 1397, 1415, 1803
MlyI GAGTC 5 cut(s) 174, 326, 626, 860, 938
MmeI TCCRAC 1 cut(s) 142
Mph1103I ATGCAT 1 cut(s) 52
MroXI GAANNNNTTC 1 cut(s) 579
MseI TTAA 2 cut(s) 230, 1466
MspA1I CMGCKG 1 cut(s) 950
MspI CCGG 3 cut(s) 43, 96, 1731
MspR9I CCNGG 2 cut(s) 543, 855
MunI CAATTG 1 cut(s) 779
MvaI CCWGG 2 cut(s) 543, 855
MwoI GCNNNNNNNGC 3 cut(s) 56, 832, 1759
NcoI CCATGG 1 cut(s) 1321
NdeI CATATG 1 cut(s) 637
NdeII GATC 5 cut(s) 16, 1306, 1331, 1634, 1725
NheI GCTAGC 1 cut(s) 35
NlaIII CATG 9 cut(s) 135, 179, 830, 848, 1262, 1325, 1362, 1433, 1508
NlaIV GGNNCC 8 cut(s) 94, 540, 852, 1327, 1394, 1395, 1396, 1773
NmuCI GTSAC 1 cut(s) 509
NsiI ATGCAT 1 cut(s) 52
NspI RCATGY 1 cut(s) 1262
NspV TTCGAA 2 cut(s) 503, 1810
PagI TCATGA 1 cut(s) 175
PaqCI CACCTGC 1 cut(s) 64
PdmI GAANNNNTTC 1 cut(s) 579
PfeI GAWTC 6 cut(s) 254, 353, 554, 647, 1289, 1807
PinAI ACCGGT 1 cut(s) 42
PkrI GCNGC 3 cut(s) 837, 1755, 1788
PleI GAGTC 5 cut(s) 173, 325, 625, 860, 937
PpsI GAGTC 5 cut(s) 173, 325, 625, 860, 937
Ppu21I YACGTR 1 cut(s) 1652
PpuMI RGGWCCY 2 cut(s) 545, 1772
Psp124BI GAGCTC 1 cut(s) 1415
Psp5II RGGWCCY 2 cut(s) 545, 1772
Psp6I CCWGG 2 cut(s) 541, 853
PspGI CCWGG 2 cut(s) 541, 853
PspN4I GGNNCC 8 cut(s) 94, 540, 852, 1327, 1394, 1395, 1396, 1773
PspOMI GGGCCC 1 cut(s) 1393
PspPI GGNCC 6 cut(s) 545, 1393, 1394, 1426, 1613, 1772
PspPPI RGGWCCY 2 cut(s) 545, 1772
PsrI GAACNNNNNNTAC 2 cut(s) 834, 866
PstNI CAGNNNCTG 1 cut(s) 578
PsuI RGATCY 1 cut(s) 1331
PvuII CAGCTG 1 cut(s) 950
RsaI GTAC 4 cut(s) 94, 314, 843, 1650
RsaNI GTAC 4 cut(s) 93, 313, 842, 1649
SacI GAGCTC 1 cut(s) 1415
SaqAI TTAA 2 cut(s) 230, 1466
SatI GCNGC 3 cut(s) 836, 1754, 1787
Sau3AI GATC 5 cut(s) 16, 1306, 1331, 1634, 1725
Sau96I GGNCC 6 cut(s) 545, 1393, 1394, 1426, 1613, 1772
SchI GAGTC 5 cut(s) 174, 326, 626, 860, 938
ScrFI CCNGG 2 cut(s) 543, 855
SduI GDGCHC 3 cut(s) 1397, 1415, 1803
SfaNI GCATC 2 cut(s) 59, 1493
SfuI TTCGAA 2 cut(s) 503, 1810
SinI GGWCC 4 cut(s) 545, 1426, 1613, 1772
SmlI CTYRAG 2 cut(s) 913, 1796
SmoI CTYRAG 2 cut(s) 913, 1796
SspI AATATT 1 cut(s) 712
SspMI CTAG 4 cut(s) 20, 36, 441, 1448
SstI GAGCTC 1 cut(s) 1415
StyD4I CCNGG 2 cut(s) 541, 853
StyI CCWWGG 4 cut(s) 627, 1321, 1619, 1683
TaaI ACNGT 2 cut(s) 407, 489
TaiI ACGT 1 cut(s) 1654
TaqI TCGA 3 cut(s) 503, 1133, 1810
TatI WGTACW 1 cut(s) 841
TfiI GAWTC 6 cut(s) 254, 353, 554, 647, 1289, 1807
Tru1I TTAA 2 cut(s) 230, 1466
Tru9I TTAA 2 cut(s) 230, 1466
TscAI CASTG 2 cut(s) 1171, 1808
TseFI GTSAC 1 cut(s) 509
TseI GCWGC 3 cut(s) 835, 1753, 1786
Tsp45I GTSAC 1 cut(s) 509
TspGWI ACGGA 1 cut(s) 296
TspRI CASTG 2 cut(s) 1171, 1808
VpaK11BI GGWCC 4 cut(s) 545, 1426, 1613, 1772
XagI CCTNNNNNAGG 1 cut(s) 18
XapI RAATTY 8 cut(s) 64, 127, 436, 721, 797, 1439, 1577, 1765
XceI RCATGY 1 cut(s) 1262
XmnI GAANNNNTTC 1 cut(s) 579
XspI CTAG 4 cut(s) 20, 36, 441, 1448
Zsp2I ATGCAT 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.