MD06G1050900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001095
Physical Location & Seq
Forward (+)
7009485 .. 7012960
3476 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1050900.v1.1.491

Sequence Viewer

Length: 1614 bp
ATGGTCCACCTTATTTTCCACACTACAATTATAATCTCAACAACTTTTCACTTCCATTGTGTTTTTTTCTTTGTGGGTTCACAGATTCTGAGCTCTCCACGCTCGCTCTCCATCGCTTCCGAGCCTTCAGATCTGAAAAACTGGTTCCCCAGCTACCAATATGAATCACCTGCACTGAATTCCAATTCTTTTCTCGATGATACCGTTGTCCTCGAAACTGAGATAGAAGAAAACTCAGGTGGGTTCGAGAGAAATGGAACAAAAAATGAAGCTTTTATTCCTAAGAACGAAACCCCAAATGGGGTTGTGAAATGTGGCAGCTTTTCTGGATATGAGCACGAAAGCCATTTGTTAAGTAAGATCCCAAGTTCTTTGGAATCAACTTCGTTGGTTTCAGAGCCCACCGACATCAGAAACTGGTACTCAAGCTATGTGTATGAGTCGCCATCGTTGGATGCAACTGATGGTGTTGGAAATTCCATTTGCGAAGTGAGTAAACACAAGGAGGATGGCTTTTTGGTTAAAAACAACAGGGGAAAAGAAAAGGTTTCGGAGGATTTTAGTACAAACAGAAGTGATTCTGAAGAGGTTAATGGGAGAGCTCCTCAGGAGGAAAGGCAGCTTGTAGGAAAGACTCCAGGTCCTTTAGAGCCGCCTTCACAACTGTCAGAGCCTACTGACATCAAAAACTGGTTCTCAAGCTATGTGTACGAGTCTTCCTCCTTTGATCCAAGTACAACTGATGGTTTTCGAGATACTGTTTGGAAAGAAAACAAAAGTGGGAAGGATGGGTTTCTGGTTGATAACAGCCACAGAGAAAATGAAGAGGACTTGGAGGGTTTTAATGAAAAGAGAAGTGATGGTGAAGCGGTTGCTGATTTACAGTCGGAAGGGCTTGCAAAATGCATCACCTCTTGCAGAGATAATAAGCAGAAAACACAGCCTGGTCGCCCTAGGGATGGAGTAAGTGAAATCCTGCCTAGTCAGAATGATTTGTGTCTCAAGCATATGCCTAAATCGTGGCAAAACCAACTCATCGCCCCAGCACAAGATGTCGAAATATCAATCTTGAATAAGGAAAGTTGTCAATGCAAGCTATCGCCTAAGACCTTCAAGGGAACCAAAATTAAGCCATCGAACAAGGAAAGAAGTTCATGCAGGAACGACGAGAAGTCTTCACAAGACATGACTCCTAGCAAGGCCTTCACTTTACAGGAGAACTCCGAAGCAGAATTCCACACAGAAGTTGATTGTATATTAACTGGAAGTGATTCAGATTTAATTCCAAGTGGAGTGTCAGAACGAAAGCCAACTCATGGAAGCAGAGACAGAAAAGATGATGGAAATGAAATATCGGAAAATGGTTTTATAACAACTAGGAAAGGCAAACTTACAAGAAACAATCAGGAAAAGATTGTGGAACGACCACAAGAGATTAGTAAAATGACAGCTCCAATAGTCGTGAAAAGAAAAGCCTTAGCAGATGCAACAAATATTCATCATTCTCCTGCGGCGGGGATTACAGGGAAATGGAAGTGTCCTCAGAAAAGTAAGCCAAATCGAGGACCCGCTTTGAAGCAGCTTCGACTTGAGAAATGGGTTCGTAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

538

Amino Acids

60.0

Weight (kDa)

5.83

Isoelectric Point (pI)

49.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 32, 1370
AarI CACCTGC 1 cut(s) 178
Acc36I ACCTGC 1 cut(s) 178
AccB7I CCANNNNNTGG 1 cut(s) 1316
AciI CCGC 5 cut(s) 653, 869, 1511, 1514, 1569
AclWI GGATC 2 cut(s) 355, 722
AcsI RAATTY 3 cut(s) 178, 475, 1232
AcuI CTGAAG 2 cut(s) 111, 603
AfaI GTAC 4 cut(s) 422, 565, 710, 736
AfiI CCNNNNNNNGG 6 cut(s) 300, 301, 959, 1316, 1514, 1562
AgsI TTSAA 3 cut(s) 1072, 1114, 1576
AhdI GACNNNNNGTC 2 cut(s) 639, 1171
AjnI CCWGG 2 cut(s) 637, 943
Alw21I GWGCWC 3 cut(s) 95, 339, 604
Alw26I GTCTC 2 cut(s) 1004, 1320
AlwI GGATC 2 cut(s) 355, 722
AlwNI CAGNNNCTG 2 cut(s) 88, 417
AoxI GGCC 1 cut(s) 1200
ApeKI GCWGC 3 cut(s) 318, 619, 1579
ApoI RAATTY 3 cut(s) 178, 475, 1232
Asp700I GAANNNNTTC 2 cut(s) 577, 1270
AspA2I CCTAGG 1 cut(s) 953
AspS9I GGNCC 3 cut(s) 4, 641, 1565
AsuHPI GGTGA 3 cut(s) 159, 875, 901
AvaII GGWCC 3 cut(s) 4, 641, 1565
AvrII CCTAGG 1 cut(s) 953
AxyI CCTNAGG 1 cut(s) 606
BaeI ACNNNNGTAYC 2 cut(s) 192, 225
BanII GRGCYC 3 cut(s) 95, 402, 604
BbsI GAAGAC 2 cut(s) 708, 1167
Bbv12I GWGCWC 3 cut(s) 95, 339, 604
BbvI GCAGC 3 cut(s) 330, 631, 1591
BciT130I CCWGG 2 cut(s) 639, 945
BcoDI GTCTC 2 cut(s) 1004, 1320
BfaI CTAG 4 cut(s) 954, 981, 1194, 1377
BfuAI ACCTGC 1 cut(s) 178
BglII AGATCT 1 cut(s) 130
BisI GCNGC 5 cut(s) 319, 620, 653, 1512, 1580
BlnI CCTAGG 1 cut(s) 953
BlsI GCNGC 5 cut(s) 320, 621, 654, 1513, 1581
Bme1390I CCNGG 2 cut(s) 639, 945
Bme18I GGWCC 3 cut(s) 4, 641, 1565
BmeRI GACNNNNNGTC 2 cut(s) 639, 1171
BmgT120I GGNCC 3 cut(s) 4, 641, 1565
BmiI GGNNCC 3 cut(s) 146, 1120, 1567
BmrFI CCNGG 2 cut(s) 639, 945
BmsI GCATC 3 cut(s) 445, 915, 1474
BpiI GAAGAC 2 cut(s) 708, 1167
BplI GAGNNNNNCTC 4 cut(s) 589, 621, 704, 736
BpmI CTGGAG 1 cut(s) 621
Bpu10I CCTNAGC 1 cut(s) 1477
BpuEI CTTGAG 4 cut(s) 409, 682, 986, 1610
BsaJI CCNNGG 1 cut(s) 953
Bsc4I CCNNNNNNNGG 6 cut(s) 300, 301, 959, 1316, 1514, 1562
Bse1I ACTGG 4 cut(s) 146, 422, 695, 1267
Bse21I CCTNAGG 1 cut(s) 606
BseBI CCWGG 2 cut(s) 639, 945
BseDI CCNNGG 1 cut(s) 953
BseGI GGATG 4 cut(s) 460, 514, 793, 964
BseLI CCNNNNNNNGG 6 cut(s) 300, 301, 959, 1316, 1514, 1562
BseMII CTCAG 5 cut(s) 80, 210, 249, 620, 1556
BseNI ACTGG 4 cut(s) 146, 422, 695, 1267
BseRI GAGGAG 1 cut(s) 594
BseXI GCAGC 3 cut(s) 330, 631, 1591
BseYI CCCAGC 2 cut(s) 149, 1042
BsgI GTGCAG 1 cut(s) 156
BshFI GGCC 1 cut(s) 1202
BsiHKAI GWGCWC 3 cut(s) 95, 339, 604
BslI CCNNNNNNNGG 6 cut(s) 300, 301, 959, 1316, 1514, 1562
BsmAI GTCTC 2 cut(s) 1004, 1320
BsnI GGCC 1 cut(s) 1202
Bsp1286I GDGCHC 4 cut(s) 95, 339, 402, 604
Bsp143I GATC 3 cut(s) 130, 360, 727
BspACI CCGC 5 cut(s) 653, 869, 1511, 1514, 1569
BspANI GGCC 1 cut(s) 1202
BspCNI CTCAG 5 cut(s) 81, 211, 248, 619, 1555
BspLI GGNNCC 3 cut(s) 146, 1120, 1567
BspMI ACCTGC 1 cut(s) 178
BspPI GGATC 2 cut(s) 355, 722
BsrI ACTGG 4 cut(s) 146, 422, 695, 1267
BssECI CCNNGG 1 cut(s) 953
BssMI GATC 3 cut(s) 130, 360, 727
BssT1I CCWWGG 1 cut(s) 953
Bst2UI CCWGG 2 cut(s) 639, 945
Bst4CI ACNGT 4 cut(s) 205, 666, 760, 885
Bst6I CTCTTC 2 cut(s) 579, 819
BstC8I GCNNGC 3 cut(s) 104, 897, 1094
BstDEI CTNAG 8 cut(s) 89, 219, 235, 282, 606, 1104, 1477, 1542
BstF5I GGATG 4 cut(s) 460, 514, 793, 964
BstKTI GATC 3 cut(s) 133, 363, 730
BstMAI GTCTC 2 cut(s) 1004, 1320
BstMBI GATC 3 cut(s) 130, 360, 727
BstMWI GCNNNNNNNGC 1 cut(s) 99
BstNI CCWGG 2 cut(s) 639, 945
BstSCI CCNGG 2 cut(s) 637, 943
BstV1I GCAGC 3 cut(s) 330, 631, 1591
BstV2I GAAGAC 2 cut(s) 708, 1167
BstX2I RGATCY 2 cut(s) 130, 360
BstYI RGATCY 2 cut(s) 130, 360
Bsu36I CCTNAGG 1 cut(s) 606
BsuRI GGCC 1 cut(s) 1202
BtgZI GCGATG 2 cut(s) 97, 1021
BtsCI GGATG 4 cut(s) 460, 514, 793, 964
BtsIMutI CAGTG 1 cut(s) 173
BveI ACCTGC 1 cut(s) 178
Cac8I GCNNGC 3 cut(s) 104, 897, 1094
CaiI CAGNNNCTG 2 cut(s) 88, 417
Cfr13I GGNCC 3 cut(s) 4, 641, 1565
Csp6I GTAC 4 cut(s) 421, 564, 709, 735
CviAII CATG 3 cut(s) 1155, 1186, 1316
CviQI GTAC 4 cut(s) 421, 564, 709, 735
DdeI CTNAG 8 cut(s) 89, 219, 235, 282, 606, 1104, 1477, 1542
DpnI GATC 3 cut(s) 132, 362, 729
DpnII GATC 3 cut(s) 130, 360, 727
DriI GACNNNNNGTC 2 cut(s) 639, 1171
Eam1104I CTCTTC 2 cut(s) 579, 819
Eam1105I GACNNNNNGTC 2 cut(s) 639, 1171
EarI CTCTTC 2 cut(s) 579, 819
Ecl136II GAGCTC 2 cut(s) 93, 602
Eco130I CCWWGG 1 cut(s) 953
Eco147I AGGCCT 1 cut(s) 1202
Eco24I GRGCYC 3 cut(s) 95, 402, 604
Eco47I GGWCC 3 cut(s) 4, 641, 1565
Eco53kI GAGCTC 2 cut(s) 93, 602
Eco57I CTGAAG 2 cut(s) 111, 603
Eco81I CCTNAGG 1 cut(s) 606
EcoICRI GAGCTC 2 cut(s) 93, 602
EcoO109I RGGNCCY 2 cut(s) 641, 1565
EcoRI GAATTC 2 cut(s) 178, 1232
EcoRII CCWGG 2 cut(s) 637, 943
EcoT14I CCWWGG 1 cut(s) 953
EcoT22I ATGCAT 1 cut(s) 908
EcoT38I GRGCYC 3 cut(s) 95, 402, 604
ErhI CCWWGG 1 cut(s) 953
FaeI CATG 3 cut(s) 1158, 1189, 1319
FalI AAGNNNNNCTT 2 cut(s) 1374, 1406
FatI CATG 3 cut(s) 1154, 1185, 1315
FauI CCCGC 2 cut(s) 1507, 1576
FauNDI CATATG 1 cut(s) 1008
Fnu4HI GCNGC 5 cut(s) 319, 620, 653, 1512, 1580
FokI GGATG 4 cut(s) 467, 521, 800, 971
FriOI GRGCYC 3 cut(s) 95, 402, 604
Fsp4HI GCNGC 5 cut(s) 319, 620, 653, 1512, 1580
FspBI CTAG 4 cut(s) 954, 981, 1194, 1377
GluI GCNGC 5 cut(s) 319, 620, 653, 1512, 1580
GsaI CCCAGC 2 cut(s) 153, 1046
GsuI CTGGAG 1 cut(s) 621
HaeIII GGCC 1 cut(s) 1202
Hin1II CATG 3 cut(s) 1158, 1189, 1319
HindIII AAGCTT 1 cut(s) 270
HinfI GANTC 9 cut(s) 85, 164, 377, 440, 578, 634, 713, 1189, 1271
HphI GGTGA 3 cut(s) 159, 875, 901
Hpy166II GTNNAC 4 cut(s) 7, 80, 497, 709
Hpy188III TCNNGA 8 cut(s) 194, 247, 327, 608, 752, 1069, 1406, 1462
Hpy8I GTNNAC 4 cut(s) 7, 80, 497, 709
Hpy99I CGWCG 1 cut(s) 1169
HpyAV CCTTC 6 cut(s) 135, 666, 778, 884, 1120, 1213
HpyCH4III ACNGT 4 cut(s) 205, 666, 760, 885
HpyCH4V TGCA 8 cut(s) 173, 458, 899, 906, 918, 1092, 1158, 1487
HpyF10VI GCNNNNNNNGC 1 cut(s) 99
HpyF3I CTNAG 8 cut(s) 89, 219, 235, 282, 606, 1104, 1477, 1542
Hsp92II CATG 3 cut(s) 1158, 1189, 1319
Kzo9I GATC 3 cut(s) 130, 360, 727
LmnI GCTCC 2 cut(s) 607, 1456
Lsp1109I GCAGC 3 cut(s) 330, 631, 1591
LweI GCATC 3 cut(s) 445, 915, 1474
MaeI CTAG 4 cut(s) 954, 981, 1194, 1377
MalI GATC 3 cut(s) 132, 362, 729
MboI GATC 3 cut(s) 130, 360, 727
MboII GAAGA 5 cut(s) 239, 596, 708, 836, 1167
MflI RGATCY 2 cut(s) 130, 360
MhlI GDGCHC 4 cut(s) 95, 339, 402, 604
MluCI AATT 7 cut(s) 27, 178, 184, 475, 1125, 1232, 1281
MlyI GAGTC 4 cut(s) 449, 628, 722, 1183
MmeI TCCRAC 3 cut(s) 432, 451, 867
Mph1103I ATGCAT 1 cut(s) 908
MroXI GAANNNNTTC 2 cut(s) 577, 1270
MseI TTAA 7 cut(s) 353, 522, 591, 843, 1128, 1259, 1280
MspR9I CCNGG 2 cut(s) 639, 945
MvaI CCWGG 2 cut(s) 639, 945
MwoI GCNNNNNNNGC 1 cut(s) 99
NdeI CATATG 1 cut(s) 1008
NdeII GATC 3 cut(s) 130, 360, 727
NlaIII CATG 3 cut(s) 1158, 1189, 1319
NlaIV GGNNCC 3 cut(s) 146, 1120, 1567
NsiI ATGCAT 1 cut(s) 908
PaqCI CACCTGC 1 cut(s) 178
PceI AGGCCT 1 cut(s) 1202
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PdmI GAANNNNTTC 2 cut(s) 577, 1270
PfeI GAWTC 5 cut(s) 85, 164, 377, 578, 1271
PflMI CCANNNNNTGG 1 cut(s) 1316
PkrI GCNGC 5 cut(s) 320, 621, 654, 1513, 1581
PleI GAGTC 4 cut(s) 448, 628, 721, 1183
PpsI GAGTC 4 cut(s) 448, 628, 721, 1183
PpuMI RGGWCCY 2 cut(s) 641, 1565
PsiI TTATAA 2 cut(s) 32, 1370
Psp124BI GAGCTC 2 cut(s) 95, 604
Psp5II RGGWCCY 2 cut(s) 641, 1565
Psp6I CCWGG 2 cut(s) 637, 943
PspFI CCCAGC 2 cut(s) 149, 1042
PspGI CCWGG 2 cut(s) 637, 943
PspN4I GGNNCC 3 cut(s) 146, 1120, 1567
PspPI GGNCC 3 cut(s) 4, 641, 1565
PspPPI RGGWCCY 2 cut(s) 641, 1565
PstNI CAGNNNCTG 2 cut(s) 88, 417
PsuI RGATCY 2 cut(s) 130, 360
RsaI GTAC 4 cut(s) 422, 565, 710, 736
RsaNI GTAC 4 cut(s) 421, 564, 709, 735
SacI GAGCTC 2 cut(s) 95, 604
SaqAI TTAA 7 cut(s) 353, 522, 591, 843, 1128, 1259, 1280
SatI GCNGC 5 cut(s) 319, 620, 653, 1512, 1580
Sau3AI GATC 3 cut(s) 130, 360, 727
Sau96I GGNCC 3 cut(s) 4, 641, 1565
SchI GAGTC 4 cut(s) 449, 628, 722, 1183
ScrFI CCNGG 2 cut(s) 639, 945
SduI GDGCHC 4 cut(s) 95, 339, 402, 604
SfaNI GCATC 3 cut(s) 445, 915, 1474
SinI GGWCC 3 cut(s) 4, 641, 1565
SmlI CTYRAG 4 cut(s) 424, 697, 1001, 1589
SmoI CTYRAG 4 cut(s) 424, 697, 1001, 1589
Sse9I AATT 7 cut(s) 27, 178, 184, 475, 1125, 1232, 1281
SseBI AGGCCT 1 cut(s) 1202
SsiI CCGC 5 cut(s) 653, 869, 1511, 1514, 1569
SspI AATATT 1 cut(s) 1495
SspMI CTAG 4 cut(s) 954, 981, 1194, 1377
SstI GAGCTC 2 cut(s) 95, 604
StuI AGGCCT 1 cut(s) 1202
StyD4I CCNGG 2 cut(s) 637, 943
StyI CCWWGG 1 cut(s) 953
TaaI ACNGT 4 cut(s) 205, 666, 760, 885
TaqI TCGA 8 cut(s) 195, 213, 246, 751, 1056, 1136, 1561, 1585
TasI AATT 7 cut(s) 27, 178, 184, 475, 1125, 1232, 1281
TatI WGTACW 2 cut(s) 563, 734
TauI GCSGC 2 cut(s) 655, 1514
TfiI GAWTC 5 cut(s) 85, 164, 377, 578, 1271
Tru1I TTAA 7 cut(s) 353, 522, 591, 843, 1128, 1259, 1280
Tru9I TTAA 7 cut(s) 353, 522, 591, 843, 1128, 1259, 1280
TscAI CASTG 1 cut(s) 180
TseI GCWGC 3 cut(s) 318, 619, 1579
TspDTI ATGAA 7 cut(s) 177, 282, 837, 861, 1143, 1362, 1487
TspRI CASTG 1 cut(s) 180
Van91I CCANNNNNTGG 1 cut(s) 1316
VpaK11BI GGWCC 3 cut(s) 4, 641, 1565
XapI RAATTY 3 cut(s) 178, 475, 1232
XmaJI CCTAGG 1 cut(s) 953
XmnI GAANNNNTTC 2 cut(s) 577, 1270
XspI CTAG 4 cut(s) 954, 981, 1194, 1377
Zsp2I ATGCAT 1 cut(s) 908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.