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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 374 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 332 |
| AciI | CCGC | 1 cut(s) | 360 |
| AclWI | GGATC | 1 cut(s) | 253 |
| AcsI | RAATTY | 1 cut(s) | 284 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 332 |
| AgsI | TTSAA | 1 cut(s) | 315 |
| AluBI | AGCT | 4 cut(s) | 17, 46, 117, 457 |
| AluI | AGCT | 4 cut(s) | 17, 46, 117, 457 |
| Alw21I | GWGCWC | 1 cut(s) | 119 |
| AlwI | GGATC | 1 cut(s) | 253 |
| ApoI | RAATTY | 1 cut(s) | 284 |
| AsuHPI | GGTGA | 1 cut(s) | 16 |
| BaeGI | GKGCMC | 1 cut(s) | 452 |
| BanII | GRGCYC | 1 cut(s) | 119 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 132, 164 |
| Bbv12I | GWGCWC | 1 cut(s) | 119 |
| BbvCI | CCTCAGC | 1 cut(s) | 453 |
| BccI | CCATC | 3 cut(s) | 68, 206, 251 |
| BclI | TGATCA | 1 cut(s) | 429 |
| BfaI | CTAG | 1 cut(s) | 47 |
| BmrI | ACTGGG | 1 cut(s) | 444 |
| BmuI | ACTGGG | 1 cut(s) | 444 |
| Bpu10I | CCTNAGC | 1 cut(s) | 453 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 180, 210 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 332 |
| Bse1I | ACTGG | 1 cut(s) | 439 |
| BseGI | GGATG | 1 cut(s) | 373 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 332 |
| BseMII | CTCAG | 1 cut(s) | 132 |
| BseNI | ACTGG | 1 cut(s) | 439 |
| BseRI | GAGGAG | 1 cut(s) | 204 |
| BseSI | GKGCMC | 1 cut(s) | 452 |
| BsgI | GTGCAG | 1 cut(s) | 85 |
| BsiHKAI | GWGCWC | 1 cut(s) | 119 |
| BsiSI | CCGG | 1 cut(s) | 419 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 332 |
| Bsp1286I | GDGCHC | 2 cut(s) | 119, 452 |
| Bsp143I | GATC | 3 cut(s) | 49, 245, 429 |
| BspACI | CCGC | 1 cut(s) | 360 |
| BspCNI | CTCAG | 1 cut(s) | 131 |
| BspPI | GGATC | 1 cut(s) | 253 |
| BsrI | ACTGG | 1 cut(s) | 439 |
| BssMI | GATC | 3 cut(s) | 49, 245, 429 |
| Bst4CI | ACNGT | 3 cut(s) | 92, 130, 346 |
| Bst6I | CTCTTC | 1 cut(s) | 316 |
| BstC8I | GCNNGC | 1 cut(s) | 358 |
| BstDEI | CTNAG | 2 cut(s) | 118, 453 |
| BstF5I | GGATG | 1 cut(s) | 373 |
| BstKTI | GATC | 3 cut(s) | 52, 248, 432 |
| BstMBI | GATC | 3 cut(s) | 49, 245, 429 |
| BstSLI | GKGCMC | 1 cut(s) | 452 |
| BtsCI | GGATG | 1 cut(s) | 373 |
| BtsIMutI | CAGTG | 1 cut(s) | 432 |
| Cac8I | GCNNGC | 1 cut(s) | 358 |
| CviJI | RGCY | 5 cut(s) | 17, 46, 117, 386, 457 |
| CviKI_1 | RGCY | 5 cut(s) | 17, 46, 117, 386, 457 |
| DdeI | CTNAG | 2 cut(s) | 118, 453 |
| DpnI | GATC | 3 cut(s) | 51, 247, 431 |
| DpnII | GATC | 3 cut(s) | 49, 245, 429 |
| Eam1104I | CTCTTC | 1 cut(s) | 316 |
| EarI | CTCTTC | 1 cut(s) | 316 |
| Ecl136II | GAGCTC | 1 cut(s) | 117 |
| Eco24I | GRGCYC | 1 cut(s) | 119 |
| Eco53kI | GAGCTC | 1 cut(s) | 117 |
| EcoICRI | GAGCTC | 1 cut(s) | 117 |
| EcoT38I | GRGCYC | 1 cut(s) | 119 |
| FaiI | YATR | 3 cut(s) | 135, 149, 374 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 370, 402 |
| FbaI | TGATCA | 1 cut(s) | 429 |
| FokI | GGATG | 1 cut(s) | 380 |
| FriOI | GRGCYC | 1 cut(s) | 119 |
| FspBI | CTAG | 1 cut(s) | 47 |
| HapII | CCGG | 1 cut(s) | 419 |
| HincII | GTYRAC | 1 cut(s) | 184 |
| HindII | GTYRAC | 1 cut(s) | 184 |
| HindIII | AAGCTT | 1 cut(s) | 15 |
| HinfI | GANTC | 3 cut(s) | 31, 98, 164 |
| HpaI | GTTAAC | 1 cut(s) | 184 |
| HpaII | CCGG | 1 cut(s) | 419 |
| HphI | GGTGA | 1 cut(s) | 16 |
| Hpy166II | GTNNAC | 3 cut(s) | 86, 184, 307 |
| Hpy188I | TCNGA | 4 cut(s) | 54, 121, 169, 253 |
| Hpy8I | GTNNAC | 3 cut(s) | 86, 184, 307 |
| HpyCH4III | ACNGT | 3 cut(s) | 92, 130, 346 |
| HpyCH4V | TGCA | 4 cut(s) | 41, 66, 137, 147 |
| HpyF3I | CTNAG | 2 cut(s) | 118, 453 |
| Ksp22I | TGATCA | 1 cut(s) | 429 |
| KspAI | GTTAAC | 1 cut(s) | 184 |
| Kzo9I | GATC | 3 cut(s) | 49, 245, 429 |
| LpnPI | CCDG | 4 cut(s) | 95, 339, 420, 432 |
| MaeI | CTAG | 1 cut(s) | 47 |
| MalI | GATC | 3 cut(s) | 51, 247, 431 |
| MboI | GATC | 3 cut(s) | 49, 245, 429 |
| MboII | GAAGA | 3 cut(s) | 152, 266, 303 |
| MhlI | GDGCHC | 2 cut(s) | 119, 452 |
| MluCI | AATT | 2 cut(s) | 174, 284 |
| MnlI | CCTC | 2 cut(s) | 182, 462 |
| MseI | TTAA | 2 cut(s) | 173, 183 |
| MspI | CCGG | 1 cut(s) | 419 |
| NdeII | GATC | 3 cut(s) | 49, 245, 429 |
| PfeI | GAWTC | 3 cut(s) | 31, 98, 164 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 332 |
| PsiI | TTATAA | 1 cut(s) | 374 |
| Psp124BI | GAGCTC | 1 cut(s) | 119 |
| SacI | GAGCTC | 1 cut(s) | 119 |
| SaqAI | TTAA | 2 cut(s) | 173, 183 |
| Sau3AI | GATC | 3 cut(s) | 49, 245, 429 |
| SduI | GDGCHC | 2 cut(s) | 119, 452 |
| SetI | ASST | 4 cut(s) | 19, 48, 119, 459 |
| SmlI | CTYRAG | 1 cut(s) | 458 |
| SmoI | CTYRAG | 1 cut(s) | 458 |
| Sse9I | AATT | 2 cut(s) | 174, 284 |
| SsiI | CCGC | 1 cut(s) | 360 |
| SspI | AATATT | 1 cut(s) | 406 |
| SspMI | CTAG | 1 cut(s) | 47 |
| SstI | GAGCTC | 1 cut(s) | 119 |
| TaaI | ACNGT | 3 cut(s) | 92, 130, 346 |
| TasI | AATT | 2 cut(s) | 174, 284 |
| TfiI | GAWTC | 3 cut(s) | 31, 98, 164 |
| Tru1I | TTAA | 2 cut(s) | 173, 183 |
| Tru9I | TTAA | 2 cut(s) | 173, 183 |
| TscAI | CASTG | 1 cut(s) | 439 |
| TspDTI | ATGAA | 2 cut(s) | 253, 398 |
| TspGWI | ACGGA | 1 cut(s) | 201 |
| TspRI | CASTG | 1 cut(s) | 439 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 332 |
| XapI | RAATTY | 1 cut(s) | 284 |
| XspI | CTAG | 1 cut(s) | 47 |