Rh7DG223400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0001095
Physical Location & Seq
Reverse (-)
21745478 .. 21749439
3962 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG223400.1

Sequence Viewer

Length: 1791 bp
ATGTCAGAACCTCCTGACCTGGGCAATTGGTTCTCAAGCTACCGCTATGAATCACCTGCAATGAATTCCAATTCTTTTCGCAATGAATTTGATACCGGCGAGATCAAAAGAGAAAGAGACGGAGGAGATTCATGTGGGTTTATGGAAAATGGAAATAAAGTTGGAGTCTTTGTTCATAAGAAGCAGAACTCAAATGGGGTTGTCAAATGTGGCAGCTCTTCACAAAACCAATGCTTAAAGCCTACGCACATCAAAAACTGGTTCTCAAGCTATGTGTATGAGTCTGCTGGTTTTGGCGATTCTCTTTGTAAAGAAAGTAAATTTGAGAAGGATGGGTTTTTGGTTGAAAACAGCAACAGAGAAAATGAAGAGGATTTGAGGAATTTTAGTACAAACAGCAGTGTTAGTAAAGAGATTGTGGGTGAGAAGGAACAGTCAGGAGAGCTTGCAAATTGTGACAGCTCTCGGAGAGATACTAAGCAGGCAAGGCAGCCTGTAAGTGAGGCTCCAGGTCCTCTGGAATCACATTTGCTCCTTTCAGAACCTATTCACATCAGAAACTGGTTCTCAAGCTATGTGTATGAGTCTCCTTCCTTGGACATGAATGATAGTTTTGGAGATTCTGTTTGCAAAGAAAGTAAATGTGAGAAGGATGGATTTAGGGTTGAAAAGAGAGATAATGAAGAGGATTTGGGGGATTTTAGAAGAAACAGTAGTGTTGGTGAAGAGGTTGCGGGAGAGAAGAAACAGTCAGTAGGGCTTGCAACATGTGACAGCTCTTGGAGAGTTACCAAGCATGATAGGCAGCCTGTAAGTAAGGTTCCTGGTCCTTTGGATTCCACTTCACACTTGTCAGAGCCTAGTCAGCCTACTGACATCAGAAACTGGTTCTCAAGCTATGTGTATGAGTCTCCTTTGGATACAGCTGATGGTTTTGGAGATTTTGGTTGTAGAGATAGAATAGATGAGAAGGATGAGGTTTTGGTTGAACCCAGTAACAAAGAAGACGGAGAGGGTTTGGAGGGTTTTAGTAAAAAGGAAAGTGATAGTGAAGAGGTTGATGTTGAGAAGGTACAGTCAGAAGAGTTCATTACCTCTTGCAGATGTAGTGTGGTTGTTGACCCAACCACTGGAGTAGATGAAAATCTATCTGGTAGGAATGATTTGTGTCTTGAATTTACACCTAAAACAGTGCAAAACCATGATACCAGTCAAGCAAAACATGGTGATACATCAAGCTTCAACAAAGGAGATTCTCAATGCAAGCGAGATCAAGAGACTTTCCATGGAACCAGATTTAAGCCATCGAACAAACAGAGAAGTATGTGCAGTAATGATGGCAAATGTCCACAAAACTTGATTCAGAGTAGCGGTTCCACTTTACAGGAGAGCTCAGAAGCAACGGTCCATGCAGAAGATTGTGTACAATTCAAAAGTGATTCAGAGTTAATTCCAGTTAAAGGAGCATCGGTAAGCAAATCAACTCATGGAAGCAAAGAAAGAAAGGATGGGGGAAAAGGAATATCAGAAGATGGTTTCATTACAACAAGGAAACGCAAATTTACAGCGAGAAATGATGAAAACTCTGCAGAGAGTAGTAAGGGAACAGTTCCATTAGCAGGCAGAAAGGATGTTGCTATTAAAAGAAAGACTTTAGCAGACACAACAAATTTTCATCATTCTCCCGCAACGATGATCACTGGGAAATGGAGGCGCCCTCAGCAACGTAAGCCAAATTTAAGACCTCCTTTGAAGCAGCTTGGACTTGAGCAATGGATTCAGAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

596

Amino Acids

66.49

Weight (kDa)

5.49

Isoelectric Point (pI)

51.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 64
Acc36I ACCTGC 1 cut(s) 64
AccB1I GGYRCC 1 cut(s) 1713
AccB7I CCANNNNNTGG 1 cut(s) 1130
AciI CCGC 4 cut(s) 43, 734, 1371, 1686
AcsI RAATTY 8 cut(s) 64, 86, 320, 382, 1175, 1559, 1669, 1735
AcyI GRCGYC 1 cut(s) 1714
AfaI GTAC 3 cut(s) 391, 1074, 1425
AfiI CCNNNNNNNGG 4 cut(s) 20, 1130, 1460, 1619
AflIII ACRYGT 1 cut(s) 767
AgsI TTSAA 7 cut(s) 347, 668, 989, 1175, 1243, 1432, 1753
AjnI CCWGG 3 cut(s) 18, 508, 823
AloI GAACNNNNNNTCC 2 cut(s) 156, 188
Alw21I GWGCWC 1 cut(s) 1394
Alw26I GTCTC 4 cut(s) 111, 591, 915, 1271
AlwNI CAGNNNCTG 2 cut(s) 561, 885
ApeKI GCWGC 4 cut(s) 213, 490, 805, 1756
ApoI RAATTY 8 cut(s) 64, 86, 320, 382, 1175, 1559, 1669, 1735
Asp700I GAANNNNTTC 1 cut(s) 546
AspLEI GCGC 1 cut(s) 1716
AspS9I GGNCC 3 cut(s) 512, 827, 1405
AsuHPI GGTGA 4 cut(s) 45, 434, 734, 1238
AvaII GGWCC 3 cut(s) 512, 827, 1405
BanI GGYRCC 1 cut(s) 1713
BanII GRGCYC 1 cut(s) 1394
BarI GAAGNNNNNNTAC 2 cut(s) 1407, 1439
BbsI GAAGAC 1 cut(s) 1011
Bbv12I GWGCWC 1 cut(s) 1394
BbvCI CCTCAGC 1 cut(s) 1719
BbvI GCAGC 4 cut(s) 225, 502, 817, 1768
BccI CCATC 7 cut(s) 326, 647, 923, 1312, 1331, 1502, 1526
BciT130I CCWGG 3 cut(s) 20, 510, 825
BciVI GTATCC 1 cut(s) 913
BclI TGATCA 1 cut(s) 1695
BcoDI GTCTC 4 cut(s) 111, 591, 915, 1271
BfaI CTAG 1 cut(s) 861
BfmI CTRYAG 1 cut(s) 1587
BfoI RGCGCY 1 cut(s) 1717
BfuAI ACCTGC 1 cut(s) 64
BfuI GTATCC 1 cut(s) 913
BisI GCNGC 4 cut(s) 214, 491, 806, 1757
BlsI GCNGC 4 cut(s) 215, 492, 807, 1758
Bme1390I CCNGG 3 cut(s) 20, 510, 825
Bme18I GGWCC 3 cut(s) 512, 827, 1405
BmgT120I GGNCC 3 cut(s) 512, 827, 1405
BmiI GGNNCC 5 cut(s) 507, 822, 1291, 1375, 1715
BmrFI CCNGG 3 cut(s) 20, 510, 825
BmrI ACTGGG 2 cut(s) 987, 1710
BmsI GCATC 1 cut(s) 1475
BmuI ACTGGG 2 cut(s) 987, 1710
BpiI GAAGAC 1 cut(s) 1011
BplI GAGNNNNNCTC 2 cut(s) 1702, 1734
BpmI CTGGAG 2 cut(s) 492, 1152
Bpu10I CCTNAGC 1 cut(s) 1719
BpuEI CTTGAG 5 cut(s) 19, 250, 553, 877, 1787
BsaBI GATNNNNATC 1 cut(s) 1143
BsaHI GRCGYC 1 cut(s) 1714
BsaJI CCNNGG 3 cut(s) 19, 594, 1285
BsaXI ACNNNNNCTCC 8 cut(s) 156, 186, 516, 546, 609, 639, 1455, 1485
Bsc4I CCNNNNNNNGG 4 cut(s) 20, 1130, 1460, 1619
Bse118I RCCGGY 1 cut(s) 95
Bse1I ACTGG 8 cut(s) 263, 566, 890, 993, 1135, 1209, 1454, 1705
Bse3DI GCAATG 3 cut(s) 66, 88, 1778
Bse8I GATNNNNATC 1 cut(s) 1143
BseBI CCWGG 3 cut(s) 20, 510, 825
BseDI CCNNGG 3 cut(s) 19, 594, 1285
BseGI GGATG 5 cut(s) 337, 658, 979, 1513, 1636
BseJI GATNNNNATC 1 cut(s) 1143
BseLI CCNNNNNNNGG 4 cut(s) 20, 1130, 1460, 1619
BseMI GCAATG 3 cut(s) 66, 88, 1778
BseMII CTCAG 2 cut(s) 1407, 1733
BseNI ACTGG 8 cut(s) 263, 566, 890, 993, 1135, 1209, 1454, 1705
BseRI GAGGAG 1 cut(s) 138
BseXI GCAGC 4 cut(s) 225, 502, 817, 1768
BsgI GTGCAG 1 cut(s) 1348
BshNI GGYRCC 1 cut(s) 1713
BsiHKAI GWGCWC 1 cut(s) 1394
BsiSI CCGG 1 cut(s) 96
BslI CCNNNNNNNGG 4 cut(s) 20, 1130, 1460, 1619
BsmAI GTCTC 4 cut(s) 111, 591, 915, 1271
BsmBI CGTCTC 1 cut(s) 111
Bsp1286I GDGCHC 1 cut(s) 1394
Bsp1407I TGTACA 1 cut(s) 1423
Bsp143I GATC 3 cut(s) 102, 1270, 1695
Bsp19I CCATGG 1 cut(s) 1285
BspACI CCGC 4 cut(s) 43, 734, 1371, 1686
BspCNI CTCAG 2 cut(s) 1406, 1732
BspLI GGNNCC 5 cut(s) 507, 822, 1291, 1375, 1715
BspMAI CTGCAG 1 cut(s) 1591
BspMI ACCTGC 1 cut(s) 64
BspQI GCTCTTC 1 cut(s) 223
BspT107I GGYRCC 1 cut(s) 1713
BsrDI GCAATG 3 cut(s) 66, 88, 1778
BsrFI RCCGGY 1 cut(s) 95
BsrGI TGTACA 1 cut(s) 1423
BsrI ACTGG 8 cut(s) 263, 566, 890, 993, 1135, 1209, 1454, 1705
BssAI RCCGGY 1 cut(s) 95
BssECI CCNNGG 3 cut(s) 19, 594, 1285
BssMI GATC 3 cut(s) 102, 1270, 1695
BssNI GRCGYC 1 cut(s) 1714
BssT1I CCWWGG 2 cut(s) 594, 1285
Bst2UI CCWGG 3 cut(s) 20, 510, 825
Bst4CI ACNGT 7 cut(s) 435, 713, 750, 1077, 1192, 1405, 1609
Bst6I CTCTTC 6 cut(s) 223, 363, 678, 720, 1047, 1077
BstACI GRCGYC 1 cut(s) 1714
BstAUI TGTACA 1 cut(s) 1423
BstC8I GCNNGC 5 cut(s) 447, 483, 762, 1265, 1621
BstDEI CTNAG 3 cut(s) 477, 1393, 1719
BstDSI CCRYGG 1 cut(s) 1285
BstF5I GGATG 5 cut(s) 337, 658, 979, 1513, 1636
BstH2I RGCGCY 1 cut(s) 1717
BstHHI GCGC 1 cut(s) 1716
BstKTI GATC 3 cut(s) 105, 1273, 1698
BstMAI GTCTC 4 cut(s) 111, 591, 915, 1271
BstMBI GATC 3 cut(s) 102, 1270, 1695
BstMWI GCNNNNNNNGC 5 cut(s) 487, 802, 865, 1720, 1729
BstNI CCWGG 3 cut(s) 20, 510, 825
BstNSI RCATGY 1 cut(s) 771
BstSCI CCNGG 3 cut(s) 18, 508, 823
BstSFI CTRYAG 1 cut(s) 1587
BstV1I GCAGC 4 cut(s) 225, 502, 817, 1768
BstV2I GAAGAC 1 cut(s) 1011
BsuI GTATCC 1 cut(s) 913
BtgI CCRYGG 1 cut(s) 1285
BtsCI GGATG 5 cut(s) 337, 658, 979, 1513, 1636
BtsI GCAGTG 1 cut(s) 406
BtsIMutI CAGTG 4 cut(s) 406, 1128, 1197, 1698
BveI ACCTGC 1 cut(s) 64
Cac8I GCNNGC 5 cut(s) 447, 483, 762, 1265, 1621
CaiI CAGNNNCTG 2 cut(s) 561, 885
CfoI GCGC 1 cut(s) 1716
Cfr10I RCCGGY 1 cut(s) 95
Cfr13I GGNCC 3 cut(s) 512, 827, 1405
Csp6I GTAC 3 cut(s) 390, 1073, 1424
CspCI CAANNNNNGTGG 2 cut(s) 1338, 1373
CviAII CATG 9 cut(s) 132, 601, 768, 797, 1202, 1223, 1286, 1409, 1487
CviQI GTAC 3 cut(s) 390, 1073, 1424
DdeI CTNAG 3 cut(s) 477, 1393, 1719
DinI GGCGCC 1 cut(s) 1715
DpnI GATC 3 cut(s) 104, 1272, 1697
DpnII GATC 3 cut(s) 102, 1270, 1695
Eam1104I CTCTTC 6 cut(s) 223, 363, 678, 720, 1047, 1077
EarI CTCTTC 6 cut(s) 223, 363, 678, 720, 1047, 1077
Ecl136II GAGCTC 1 cut(s) 1392
Eco130I CCWWGG 2 cut(s) 594, 1285
Eco24I GRGCYC 1 cut(s) 1394
Eco47I GGWCC 3 cut(s) 512, 827, 1405
Eco53kI GAGCTC 1 cut(s) 1392
EcoICRI GAGCTC 1 cut(s) 1392
EcoO109I RGGNCCY 1 cut(s) 512
EcoRI GAATTC 1 cut(s) 64
EcoRII CCWGG 3 cut(s) 18, 508, 823
EcoT14I CCWWGG 2 cut(s) 594, 1285
EcoT38I GRGCYC 1 cut(s) 1394
EgeI GGCGCC 1 cut(s) 1715
EheI GGCGCC 1 cut(s) 1715
ErhI CCWWGG 2 cut(s) 594, 1285
Esp3I CGTCTC 1 cut(s) 111
FaeI CATG 9 cut(s) 135, 604, 771, 800, 1205, 1226, 1289, 1412, 1490
FalI AAGNNNNNCTT 4 cut(s) 1636, 1668, 1732, 1764
FatI CATG 9 cut(s) 131, 600, 767, 796, 1201, 1222, 1285, 1408, 1486
FauI CCCGC 2 cut(s) 727, 1693
FbaI TGATCA 1 cut(s) 1695
Fnu4HI GCNGC 4 cut(s) 214, 491, 806, 1757
FokI GGATG 5 cut(s) 344, 665, 986, 1520, 1643
FriOI GRGCYC 1 cut(s) 1394
Fsp4HI GCNGC 4 cut(s) 214, 491, 806, 1757
FspBI CTAG 1 cut(s) 861
GlaI GCGC 1 cut(s) 1715
GluI GCNGC 4 cut(s) 214, 491, 806, 1757
GsuI CTGGAG 2 cut(s) 492, 1152
HaeII RGCGCY 1 cut(s) 1717
HapII CCGG 1 cut(s) 96
HhaI GCGC 1 cut(s) 1716
Hin1I GRCGYC 1 cut(s) 1714
Hin1II CATG 9 cut(s) 135, 604, 771, 800, 1205, 1226, 1289, 1412, 1490
Hin6I GCGC 1 cut(s) 1714
HinP1I GCGC 1 cut(s) 1714
HincII GTYRAC 1 cut(s) 1120
HindII GTYRAC 1 cut(s) 1120
HindIII AAGCTT 1 cut(s) 1237
HpaII CCGG 1 cut(s) 96
HphI GGTGA 4 cut(s) 45, 434, 734, 1238
Hpy166II GTNNAC 3 cut(s) 1120, 1349, 1424
Hpy188III TCNNGA 5 cut(s) 14, 438, 518, 1172, 1274
Hpy8I GTNNAC 3 cut(s) 1120, 1349, 1424
HpyAV CCTTC 6 cut(s) 322, 421, 600, 643, 964, 1063
HpyCH4III ACNGT 7 cut(s) 435, 713, 750, 1077, 1192, 1405, 1609
HpyCH4IV ACGT 1 cut(s) 1726
HpyF10VI GCNNNNNNNGC 5 cut(s) 487, 802, 865, 1720, 1729
HpyF3I CTNAG 3 cut(s) 477, 1393, 1719
HpySE526I ACGT 1 cut(s) 1726
Hsp92I GRCGYC 1 cut(s) 1714
Hsp92II CATG 9 cut(s) 135, 604, 771, 800, 1205, 1226, 1289, 1412, 1490
HspAI GCGC 1 cut(s) 1714
KasI GGCGCC 1 cut(s) 1713
Ksp22I TGATCA 1 cut(s) 1695
Kzo9I GATC 3 cut(s) 102, 1270, 1695
LguI GCTCTTC 1 cut(s) 223
LmnI GCTCC 3 cut(s) 511, 537, 1463
Lsp1109I GCAGC 4 cut(s) 225, 502, 817, 1768
LweI GCATC 1 cut(s) 1475
MaeI CTAG 1 cut(s) 861
MaeII ACGT 1 cut(s) 1726
MaeIII GTNAC 4 cut(s) 455, 770, 787, 995
MalI GATC 3 cut(s) 104, 1272, 1697
MboI GATC 3 cut(s) 102, 1270, 1695
MfeI CAATTG 1 cut(s) 25
MhlI GDGCHC 1 cut(s) 1394
Mly113I GGCGCC 1 cut(s) 1714
MlyI GAGTC 4 cut(s) 174, 290, 593, 917
MmeI TCCRAC 1 cut(s) 142
MroXI GAANNNNTTC 1 cut(s) 546
MseI TTAA 6 cut(s) 236, 1299, 1448, 1458, 1641, 1739
MspA1I CMGCKG 1 cut(s) 926
MspI CCGG 1 cut(s) 96
MspR9I CCNGG 3 cut(s) 20, 510, 825
MunI CAATTG 1 cut(s) 25
MvaI CCWGG 3 cut(s) 20, 510, 825
MwoI GCNNNNNNNGC 5 cut(s) 487, 802, 865, 1720, 1729
NarI GGCGCC 1 cut(s) 1714
NcoI CCATGG 1 cut(s) 1285
NdeII GATC 3 cut(s) 102, 1270, 1695
NlaIII CATG 9 cut(s) 135, 604, 771, 800, 1205, 1226, 1289, 1412, 1490
NlaIV GGNNCC 5 cut(s) 507, 822, 1291, 1375, 1715
NmuCI GTSAC 2 cut(s) 455, 770
NspI RCATGY 1 cut(s) 771
PaqCI CACCTGC 1 cut(s) 64
PciI ACATGT 1 cut(s) 767
PciSI GCTCTTC 1 cut(s) 223
PdmI GAANNNNTTC 1 cut(s) 546
PflMI CCANNNNNTGG 1 cut(s) 1130
PkrI GCNGC 4 cut(s) 215, 492, 807, 1758
PleI GAGTC 4 cut(s) 173, 289, 592, 916
PluTI GGCGCC 1 cut(s) 1717
PpsI GAGTC 4 cut(s) 173, 289, 592, 916
PpuMI RGGWCCY 1 cut(s) 512
PscI ACATGT 1 cut(s) 767
Psp124BI GAGCTC 1 cut(s) 1394
Psp5II RGGWCCY 1 cut(s) 512
Psp6I CCWGG 3 cut(s) 18, 508, 823
PspGI CCWGG 3 cut(s) 18, 508, 823
PspN4I GGNNCC 5 cut(s) 507, 822, 1291, 1375, 1715
PspPI GGNCC 3 cut(s) 512, 827, 1405
PspPPI RGGWCCY 1 cut(s) 512
PsrI GAACNNNNNNTAC 2 cut(s) 804, 836
PstI CTGCAG 1 cut(s) 1591
PstNI CAGNNNCTG 2 cut(s) 561, 885
PvuII CAGCTG 1 cut(s) 926
RsaI GTAC 3 cut(s) 391, 1074, 1425
RsaNI GTAC 3 cut(s) 390, 1073, 1424
SacI GAGCTC 1 cut(s) 1394
SapI GCTCTTC 1 cut(s) 223
SaqAI TTAA 6 cut(s) 236, 1299, 1448, 1458, 1641, 1739
SatI GCNGC 4 cut(s) 214, 491, 806, 1757
Sau3AI GATC 3 cut(s) 102, 1270, 1695
Sau96I GGNCC 3 cut(s) 512, 827, 1405
SchI GAGTC 4 cut(s) 174, 290, 593, 917
ScrFI CCNGG 3 cut(s) 20, 510, 825
SduI GDGCHC 1 cut(s) 1394
SfaNI GCATC 1 cut(s) 1475
SfcI CTRYAG 1 cut(s) 1587
SfoI GGCGCC 1 cut(s) 1715
SinI GGWCC 3 cut(s) 512, 827, 1405
SmlI CTYRAG 5 cut(s) 34, 265, 568, 892, 1766
SmoI CTYRAG 5 cut(s) 34, 265, 568, 892, 1766
SsiI CCGC 4 cut(s) 43, 734, 1371, 1686
SspDI GGCGCC 1 cut(s) 1713
SspMI CTAG 1 cut(s) 861
SstI GAGCTC 1 cut(s) 1394
StyD4I CCNGG 3 cut(s) 18, 508, 823
StyI CCWWGG 2 cut(s) 594, 1285
TaaI ACNGT 7 cut(s) 435, 713, 750, 1077, 1192, 1405, 1609
TaiI ACGT 1 cut(s) 1729
TaqI TCGA 1 cut(s) 1307
TatI WGTACW 2 cut(s) 389, 1423
Tru1I TTAA 6 cut(s) 236, 1299, 1448, 1458, 1641, 1739
Tru9I TTAA 6 cut(s) 236, 1299, 1448, 1458, 1641, 1739
TscAI CASTG 4 cut(s) 406, 1135, 1197, 1705
TseFI GTSAC 2 cut(s) 455, 770
TseI GCWGC 4 cut(s) 213, 490, 805, 1756
Tsp45I GTSAC 2 cut(s) 455, 770
TspGWI ACGGA 2 cut(s) 135, 1023
TspRI CASTG 4 cut(s) 406, 1135, 1197, 1705
Van91I CCANNNNNTGG 1 cut(s) 1130
VpaK11BI GGWCC 3 cut(s) 512, 827, 1405
XapI RAATTY 8 cut(s) 64, 86, 320, 382, 1175, 1559, 1669, 1735
XceI RCATGY 1 cut(s) 771
XmnI GAANNNNTTC 1 cut(s) 546
XspI CTAG 1 cut(s) 861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.