Rorug03G0305000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001095
Physical Location & Seq
Forward (+)
32359810 .. 32369314
9505 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0305000.1

Sequence Viewer

Length: 1236 bp
ATGGAGTTCGAGAAGATCAAGGTCGCCAATCCCATCGTTGAGATGGACGGAGATGAGATGACTCGAGTTTTCTGGAAATCAATTAAGGATAAGCTTATTTTCCCCTTTTTGGATCTGGACATCAAGTACTTTGACCTTGGTCTTCCTCATCGTGATGCCACTAATGATAAAGTTACTGTTGAAAGTGCAGAGGCAACTCTTAGGTACAATGTAGCAATCAAATGCGCCACTGTTTCAACAGATGCCAATCGCATGAAGGAGTATAACTTAAAGCAACTGTGGAGGAGTCCCAATGTGACTATTAGGAACATTTTGAATGGTACTGTTTTCAGAGAACCGATTCTTTGCAAGAACGTTCCTCGCCTTATCCCAGGCTGGACAAAGGCAATATGCATTGGAAGACATGCTTTCGGTGATCAATATCGATCAATTGATTTAGTCATAAAAGGACCTGGGAAACTGAAATTGCTTTTTGTTCCAGAAGGAAAGGATGAGAAGACTGAACTGGATGTATACAACTTTACAGGGGAAGGGGGAGTTGCACTTGCCATGTGCAACGTTGATGAGTCAATCCGTGCTTTTGCAGAGGCTTCTATGACCACAGCTTATGAGAAAAAGTGGCCTCTTTATCTTAGCACAAAAAATACTGTTCTTAAGAAGTACGATGGAAGATTCAAGGACATATTTCAAGAAGTATATGAAGCCCAGTGGAAATCTAAGTATGAAGCTGCTGGCATATGGTATGAACATCGTCTCATTGATGATATGGTGGCTTATGCACTTAAAAGCGAAGGTGGTTTTGTTTGGGCATGCAAGAATTATGATGGAGATGTACAAAGTGATTTCTTAGCCCAAGGTTTTGGATCTCTTGGATTGATGACTTCAGTACTGGTGTGCCCAGATGGAAAGACTATAGAAGCTGAAGCCGCCCATGGTACAGTTACTCGCCATTATAGAATCCACCAAAAAGGAGGTGAAACCAGCACAAACAGCATAGCTTCTATTTTTGCTTGGACAAGAGGGCTTGCACACAGGGCAAAGTTGGATGACAATGCTAAACTGTTGGATTTCACTCAGAAGCTGGAAGCAGCTTGTATTGGAACTGTGGAATCTGGGAAAATGACCAAGGATCTTGCGCTAAATATTCATGGACCTAAGGTTGCTAGGAACCACTACTTGAACACGGAAGTGTTCATTGATGCCGTCGCAGCAGAACTGAGAGCAAAGCTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

411

Amino Acids

46.23

Weight (kDa)

7.58

Isoelectric Point (pI)

21.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Iso_dh PF00180 7 - 402 7.1e-78 Isocitrate/isopropylmalate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 513
AciI CCGC 1 cut(s) 927
AclI AACGTT 2 cut(s) 354, 558
AclWI GGATC 3 cut(s) 120, 871, 1137
AcuI CTGAAG 2 cut(s) 867, 942
AfaI GTAC 7 cut(s) 128, 206, 322, 662, 834, 888, 937
AfiI CCNNNNNNNGG 1 cut(s) 109
AflII CTTAAG 1 cut(s) 653
AgsI TTSAA 6 cut(s) 182, 237, 316, 676, 689, 1180
AjnI CCWGG 2 cut(s) 370, 451
AleI CACNNNNGTG 1 cut(s) 1187
AluBI AGCT 8 cut(s) 94, 605, 728, 920, 998, 1081, 1091, 1228
AluI AGCT 8 cut(s) 94, 605, 728, 920, 998, 1081, 1091, 1228
Alw26I GTCTC 1 cut(s) 758
AlwI GGATC 3 cut(s) 120, 871, 1137
AlwNI CAGNNNCTG 1 cut(s) 1081
Ama87I CYCGRG 1 cut(s) 63
AoxI GGCC 1 cut(s) 620
ApeKI GCWGC 3 cut(s) 728, 1088, 1208
Asp700I GAANNNNTTC 1 cut(s) 339
AspLEI GCGC 2 cut(s) 227, 1138
AspS9I GGNCC 2 cut(s) 449, 1151
AsuHPI GGTGA 2 cut(s) 425, 986
AvaI CYCGRG 1 cut(s) 63
AvaII GGWCC 2 cut(s) 449, 1151
AxyI CCTNAGG 1 cut(s) 1155
BaeGI GKGCMC 1 cut(s) 899
BbsI GAAGAC 3 cut(s) 134, 406, 503
BbvI GCAGC 3 cut(s) 715, 1100, 1220
BccI CCATC 5 cut(s) 37, 41, 659, 818, 896
BceAI ACGGC 1 cut(s) 1187
BciT130I CCWGG 2 cut(s) 372, 453
BclI TGATCA 1 cut(s) 415
BcoDI GTCTC 1 cut(s) 758
BfaI CTAG 1 cut(s) 1164
BfmI CTRYAG 1 cut(s) 912
BfrI CTTAAG 1 cut(s) 653
BisI GCNGC 4 cut(s) 729, 927, 1089, 1209
BlsI GCNGC 4 cut(s) 730, 928, 1090, 1210
BmcAI AGTACT 2 cut(s) 128, 888
Bme1390I CCNGG 2 cut(s) 372, 453
Bme18I GGWCC 2 cut(s) 449, 1151
BmeT110I CYCGRG 1 cut(s) 63
BmgT120I GGNCC 2 cut(s) 449, 1151
BmiI GGNNCC 1 cut(s) 1169
BmrFI CCNGG 2 cut(s) 372, 453
BmrI ACTGGG 1 cut(s) 700
BmsI GCATC 3 cut(s) 145, 232, 1189
BmuI ACTGGG 1 cut(s) 700
BoxI GACNNNNGTC 1 cut(s) 138
BpiI GAAGAC 3 cut(s) 134, 406, 503
Bsa29I ATCGAT 1 cut(s) 424
BsaBI GATNNNNATC 2 cut(s) 246, 420
BsaJI CCNNGG 6 cut(s) 136, 370, 452, 853, 931, 1125
Bsc4I CCNNNNNNNGG 1 cut(s) 109
Bse1I ACTGG 3 cut(s) 510, 706, 894
Bse21I CCTNAGG 1 cut(s) 1155
Bse8I GATNNNNATC 2 cut(s) 246, 420
BseBI CCWGG 2 cut(s) 372, 453
BseCI ATCGAT 1 cut(s) 424
BseDI CCNNGG 6 cut(s) 136, 370, 452, 853, 931, 1125
BseGI GGATG 3 cut(s) 496, 514, 1051
BseJI GATNNNNATC 2 cut(s) 246, 420
BseLI CCNNNNNNNGG 1 cut(s) 109
BseMII CTCAG 2 cut(s) 1088, 1208
BseNI ACTGG 3 cut(s) 510, 706, 894
BseRI GAGGAG 1 cut(s) 298
BseSI GKGCMC 1 cut(s) 899
BseXI GCAGC 3 cut(s) 715, 1100, 1220
BsgI GTGCAG 1 cut(s) 207
BshFI GGCC 1 cut(s) 622
BshVI ATCGAT 1 cut(s) 424
BsiHKCI CYCGRG 1 cut(s) 63
BslFI GGGAC 1 cut(s) 273
BslI CCNNNNNNNGG 1 cut(s) 109
BsmAI GTCTC 1 cut(s) 758
BsmBI CGTCTC 1 cut(s) 758
BsmFI GGGAC 1 cut(s) 273
BsnI GGCC 1 cut(s) 622
BsoBI CYCGRG 1 cut(s) 63
Bsp1286I GDGCHC 1 cut(s) 899
Bsp1407I TGTACA 1 cut(s) 832
Bsp143I GATC 6 cut(s) 15, 112, 415, 425, 863, 1129
Bsp19I CCATGG 1 cut(s) 931
BspACI CCGC 1 cut(s) 927
BspANI GGCC 1 cut(s) 622
BspCNI CTCAG 2 cut(s) 1087, 1209
BspDI ATCGAT 1 cut(s) 424
BspLI GGNNCC 1 cut(s) 1169
BspPI GGATC 3 cut(s) 120, 871, 1137
BspTI CTTAAG 1 cut(s) 653
BsrGI TGTACA 1 cut(s) 832
BsrI ACTGG 3 cut(s) 510, 706, 894
BssECI CCNNGG 6 cut(s) 136, 370, 452, 853, 931, 1125
BssMI GATC 6 cut(s) 15, 112, 415, 425, 863, 1129
BssNAI GTATAC 1 cut(s) 514
BssT1I CCWWGG 4 cut(s) 136, 853, 931, 1125
Bst1107I GTATAC 1 cut(s) 514
Bst2UI CCWGG 2 cut(s) 372, 453
Bst4CI ACNGT 8 cut(s) 178, 232, 279, 325, 649, 940, 1062, 1105
BstAFI CTTAAG 1 cut(s) 653
BstAUI TGTACA 1 cut(s) 832
BstC8I GCNNGC 3 cut(s) 733, 811, 1026
BstDEI CTNAG 7 cut(s) 200, 632, 717, 847, 1074, 1155, 1217
BstDSI CCRYGG 1 cut(s) 931
BstF5I GGATG 3 cut(s) 496, 514, 1051
BstHHI GCGC 2 cut(s) 227, 1138
BstKTI GATC 6 cut(s) 18, 115, 418, 428, 866, 1132
BstMAI GTCTC 1 cut(s) 758
BstMBI GATC 6 cut(s) 15, 112, 415, 425, 863, 1129
BstMWI GCNNNNNNNGC 4 cut(s) 926, 990, 1034, 1208
BstNI CCWGG 2 cut(s) 372, 453
BstNSI RCATGY 2 cut(s) 407, 813
BstPAI GACNNNNGTC 1 cut(s) 138
BstSCI CCNGG 2 cut(s) 370, 451
BstSFI CTRYAG 1 cut(s) 912
BstSLI GKGCMC 1 cut(s) 899
BstV1I GCAGC 3 cut(s) 715, 1100, 1220
BstV2I GAAGAC 3 cut(s) 134, 406, 503
BstX2I RGATCY 3 cut(s) 112, 863, 1129
BstXI CCANNNNNNTGG 1 cut(s) 860
BstYI RGATCY 3 cut(s) 112, 863, 1129
BstZ17I GTATAC 1 cut(s) 514
Bsu15I ATCGAT 1 cut(s) 424
Bsu36I CCTNAGG 1 cut(s) 1155
BsuRI GGCC 1 cut(s) 622
BsuTUI ATCGAT 1 cut(s) 424
BtgI CCRYGG 1 cut(s) 931
BtsCI GGATG 3 cut(s) 496, 514, 1051
BtsIMutI CAGTG 2 cut(s) 228, 713
Cac8I GCNNGC 3 cut(s) 733, 811, 1026
CaiI CAGNNNCTG 1 cut(s) 1081
CfoI GCGC 2 cut(s) 227, 1138
Cfr13I GGNCC 2 cut(s) 449, 1151
ClaI ATCGAT 1 cut(s) 424
Csp6I GTAC 7 cut(s) 127, 205, 321, 661, 833, 887, 936
CviAII CATG 6 cut(s) 253, 404, 550, 810, 932, 1148
CviQI GTAC 7 cut(s) 127, 205, 321, 661, 833, 887, 936
DdeI CTNAG 7 cut(s) 200, 632, 717, 847, 1074, 1155, 1217
DpnI GATC 6 cut(s) 17, 114, 417, 427, 865, 1131
DpnII GATC 6 cut(s) 15, 112, 415, 425, 863, 1129
Eco130I CCWWGG 4 cut(s) 136, 853, 931, 1125
Eco47I GGWCC 2 cut(s) 449, 1151
Eco57I CTGAAG 2 cut(s) 867, 942
Eco81I CCTNAGG 1 cut(s) 1155
Eco88I CYCGRG 1 cut(s) 63
EcoO109I RGGNCCY 1 cut(s) 449
EcoRII CCWGG 2 cut(s) 370, 451
EcoT14I CCWWGG 4 cut(s) 136, 853, 931, 1125
EcoT22I ATGCAT 1 cut(s) 395
ErhI CCWWGG 4 cut(s) 136, 853, 931, 1125
Esp3I CGTCTC 1 cut(s) 758
FaeI CATG 6 cut(s) 256, 407, 553, 813, 935, 1151
FalI AAGNNNNNCTT 2 cut(s) 391, 423
FaqI GGGAC 1 cut(s) 273
FatI CATG 6 cut(s) 252, 403, 549, 809, 931, 1147
FauNDI CATATG 1 cut(s) 737
FbaI TGATCA 1 cut(s) 415
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 4 cut(s) 729, 927, 1089, 1209
FokI GGATG 3 cut(s) 503, 521, 1058
Fsp4HI GCNGC 4 cut(s) 729, 927, 1089, 1209
FspBI CTAG 1 cut(s) 1164
GlaI GCGC 2 cut(s) 226, 1137
GluI GCNGC 4 cut(s) 729, 927, 1089, 1209
HaeIII GGCC 1 cut(s) 622
HhaI GCGC 2 cut(s) 227, 1138
Hin1II CATG 6 cut(s) 256, 407, 553, 813, 935, 1151
Hin6I GCGC 2 cut(s) 225, 1136
HinP1I GCGC 2 cut(s) 225, 1136
HindIII AAGCTT 2 cut(s) 92, 1226
HinfI GANTC 7 cut(s) 61, 286, 340, 566, 672, 957, 1109
HphI GGTGA 2 cut(s) 425, 986
Hpy166II GTNNAC 1 cut(s) 514
Hpy188I TCNGA 2 cut(s) 332, 1077
Hpy188III TCNNGA 7 cut(s) 10, 73, 116, 152, 479, 689, 1233
Hpy8I GTNNAC 1 cut(s) 514
Hpy99I CGWCG 1 cut(s) 1208
HpyAV CCTTC 4 cut(s) 250, 476, 524, 785
HpyCH4III ACNGT 8 cut(s) 178, 232, 279, 325, 649, 940, 1062, 1105
HpyCH4IV ACGT 2 cut(s) 354, 558
HpyCH4V TGCA 9 cut(s) 188, 348, 393, 542, 555, 584, 779, 813, 1028
HpyF10VI GCNNNNNNNGC 4 cut(s) 926, 990, 1034, 1208
HpyF3I CTNAG 7 cut(s) 200, 632, 717, 847, 1074, 1155, 1217
HpySE526I ACGT 2 cut(s) 354, 558
Hsp92II CATG 6 cut(s) 256, 407, 553, 813, 935, 1151
HspAI GCGC 2 cut(s) 225, 1136
Ksp22I TGATCA 1 cut(s) 415
Kzo9I GATC 6 cut(s) 15, 112, 415, 425, 863, 1129
Lsp1109I GCAGC 3 cut(s) 715, 1100, 1220
LweI GCATC 3 cut(s) 145, 232, 1189
MaeI CTAG 1 cut(s) 1164
MaeII ACGT 2 cut(s) 354, 558
MaeIII GTNAC 3 cut(s) 172, 295, 940
MalI GATC 6 cut(s) 17, 114, 417, 427, 865, 1131
MboI GATC 6 cut(s) 15, 112, 415, 425, 863, 1129
MboII GAAGA 5 cut(s) 25, 134, 411, 508, 681
MfeI CAATTG 1 cut(s) 429
MflI RGATCY 3 cut(s) 112, 863, 1129
MhlI GDGCHC 1 cut(s) 899
MluCI AATT 4 cut(s) 81, 429, 464, 817
MlyI GAGTC 3 cut(s) 55, 295, 575
MmeI TCCRAC 2 cut(s) 1023, 1044
MnlI CCTC 8 cut(s) 156, 184, 276, 369, 580, 633, 965, 1013
Mph1103I ATGCAT 1 cut(s) 395
MroXI GAANNNNTTC 1 cut(s) 339
MseI TTAA 4 cut(s) 84, 269, 654, 783
MslI CAYNNNNRTG 2 cut(s) 153, 1187
MspCI CTTAAG 1 cut(s) 653
MspR9I CCNGG 2 cut(s) 372, 453
MunI CAATTG 1 cut(s) 429
MvaI CCWGG 2 cut(s) 372, 453
MwoI GCNNNNNNNGC 4 cut(s) 926, 990, 1034, 1208
NcoI CCATGG 1 cut(s) 931
NdeI CATATG 1 cut(s) 737
NdeII GATC 6 cut(s) 15, 112, 415, 425, 863, 1129
NlaIII CATG 6 cut(s) 256, 407, 553, 813, 935, 1151
NlaIV GGNNCC 1 cut(s) 1169
NmuCI GTSAC 1 cut(s) 295
NsiI ATGCAT 1 cut(s) 395
NspI RCATGY 2 cut(s) 407, 813
OliI CACNNNNGTG 1 cut(s) 1187
PaeI GCATGC 1 cut(s) 813
PaeR7I CTCGAG 1 cut(s) 63
PdmI GAANNNNTTC 1 cut(s) 339
PfeI GAWTC 4 cut(s) 340, 672, 957, 1109
PkrI GCNGC 4 cut(s) 730, 928, 1090, 1210
PleI GAGTC 3 cut(s) 55, 294, 574
PpsI GAGTC 3 cut(s) 55, 294, 574
PpuMI RGGWCCY 1 cut(s) 449
PshAI GACNNNNGTC 1 cut(s) 138
Psp1406I AACGTT 2 cut(s) 354, 558
Psp5II RGGWCCY 1 cut(s) 449
Psp6I CCWGG 2 cut(s) 370, 451
PspGI CCWGG 2 cut(s) 370, 451
PspN4I GGNNCC 1 cut(s) 1169
PspPI GGNCC 2 cut(s) 449, 1151
PspPPI RGGWCCY 1 cut(s) 449
PspXI VCTCGAGB 1 cut(s) 63
PstNI CAGNNNCTG 1 cut(s) 1081
PsuI RGATCY 3 cut(s) 112, 863, 1129
RsaI GTAC 7 cut(s) 128, 206, 322, 662, 834, 888, 937
RsaNI GTAC 7 cut(s) 127, 205, 321, 661, 833, 887, 936
RseI CAYNNNNRTG 2 cut(s) 153, 1187
SaqAI TTAA 4 cut(s) 84, 269, 654, 783
SatI GCNGC 4 cut(s) 729, 927, 1089, 1209
Sau3AI GATC 6 cut(s) 15, 112, 415, 425, 863, 1129
Sau96I GGNCC 2 cut(s) 449, 1151
ScaI AGTACT 2 cut(s) 128, 888
SchI GAGTC 3 cut(s) 55, 295, 575
ScrFI CCNGG 2 cut(s) 372, 453
SduI GDGCHC 1 cut(s) 899
SfaNI GCATC 3 cut(s) 145, 232, 1189
SfcI CTRYAG 1 cut(s) 912
Sfr274I CTCGAG 1 cut(s) 63
SinI GGWCC 2 cut(s) 449, 1151
SlaI CTCGAG 1 cut(s) 63
SmiMI CAYNNNNRTG 2 cut(s) 153, 1187
SmlI CTYRAG 2 cut(s) 63, 653
SmoI CTYRAG 2 cut(s) 63, 653
SphI GCATGC 1 cut(s) 813
Sse9I AATT 4 cut(s) 81, 429, 464, 817
SsiI CCGC 1 cut(s) 927
SspI AATATT 1 cut(s) 1144
SspMI CTAG 1 cut(s) 1164
StyD4I CCNGG 2 cut(s) 370, 451
StyI CCWWGG 4 cut(s) 136, 853, 931, 1125
TaaI ACNGT 8 cut(s) 178, 232, 279, 325, 649, 940, 1062, 1105
TaiI ACGT 2 cut(s) 357, 561
TaqI TCGA 3 cut(s) 9, 64, 424
TasI AATT 4 cut(s) 81, 429, 464, 817
TatI WGTACW 3 cut(s) 126, 832, 886
TauI GCSGC 1 cut(s) 929
TfiI GAWTC 4 cut(s) 340, 672, 957, 1109
Tru1I TTAA 4 cut(s) 84, 269, 654, 783
Tru9I TTAA 4 cut(s) 84, 269, 654, 783
TscAI CASTG 2 cut(s) 235, 713
TseFI GTSAC 1 cut(s) 295
TseI GCWGC 3 cut(s) 728, 1088, 1208
Tsp45I GTSAC 1 cut(s) 295
TspDTI ATGAA 6 cut(s) 269, 714, 738, 759, 1136, 1183
TspGWI ACGGA 3 cut(s) 63, 563, 1199
TspRI CASTG 2 cut(s) 235, 713
Vha464I CTTAAG 1 cut(s) 653
VpaK11BI GGWCC 2 cut(s) 449, 1151
XceI RCATGY 2 cut(s) 407, 813
XcmI CCANNNNNNNNNTGG 1 cut(s) 40
XhoI CTCGAG 1 cut(s) 63
XmiI GTMKAC 1 cut(s) 513
XmnI GAANNNNTTC 1 cut(s) 339
XspI CTAG 1 cut(s) 1164
ZrmI AGTACT 2 cut(s) 128, 888
Zsp2I ATGCAT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.