Rh4AG008300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001095
Physical Location & Seq
Reverse (-)
1432021 .. 1452658
20638 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG008300.1

Sequence Viewer

Length: 660 bp
ATGGCCGCGCCGGCAACGAGAACGCCTTCTACTGGCTCACCGCCACCGAGCACCACTTCGGCTACCTCGACATCCCCGCCGCCGACTGAGTCAAGATCGCTGCCACTCGCTTGCGCGGCGACCAAAGCCCCTGGATGTGTTGGTACGAGTCTCGTTTCAGGTTCTTCGGAGTCACTTTCGTCCCTTTCAGAGCCTACGCATATCAAAAACTGGTTCTCAAGCTATGTGTATGAGTCTCCTTCATTGGATACAACTGATGGTTTTGGCGATTCTTTTTGCAAAAAAAGAATTTTAGAGGTTGTGGGTGAGAAGAAACGGTCAGGAGGGCTTGCAAATTGTGACAGCTCTTCGAGAGATACTAAGCAGACAAGGCAGACTCTAAGTGAGGCTCCAGGTCCTGTGGAATCACTTTTGTTCCTTTCAGAATGTAAATGTAAGGATGGATTCATGGTTGAAAATAACAAGAGAGATAATGAAGAGGATTTGGGGGATTTTAGAAGAAAGAGTTGTGTTGGTGAAGAGGTTGTGGGAGAGAAGAAACAGTCAGTATGGCTTGCAATATGTGACAGCTCTTGGAGAGATACCAAGCATGGTAGCAGCTTGTTCCAGGTCCTTTGGATTCCTCTTCACACTAGTCAGGTTTGTATGAGTTACTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

219

Amino Acids

23.59

Weight (kDa)

5.97

Isoelectric Point (pI)

75.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 8, 116
AciI CCGC 5 cut(s) 6, 41, 77, 80, 116
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 288
AfaI GTAC 1 cut(s) 145
AfiI CCNNNNNNNGG 1 cut(s) 32
AgsI TTSAA 1 cut(s) 455
AhlI ACTAGT 1 cut(s) 632
AjnI CCWGG 3 cut(s) 130, 391, 606
AluBI AGCT 4 cut(s) 222, 345, 570, 600
AluI AGCT 4 cut(s) 222, 345, 570, 600
Alw21I GWGCWC 1 cut(s) 53
Alw26I GTCTC 2 cut(s) 155, 240
AlwNI CAGNNNCTG 1 cut(s) 398
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 100, 597
ApoI RAATTY 1 cut(s) 288
Asp700I GAANNNNTTC 1 cut(s) 25
AspLEI GCGC 2 cut(s) 10, 116
AspS9I GGNCC 2 cut(s) 395, 610
AsuHPI GGTGA 3 cut(s) 30, 317, 527
AvaII GGWCC 2 cut(s) 395, 610
Bbv12I GWGCWC 1 cut(s) 53
BbvI GCAGC 2 cut(s) 87, 609
BccI CCATC 2 cut(s) 251, 434
BciT130I CCWGG 3 cut(s) 132, 393, 608
BciVI GTATCC 1 cut(s) 241
BcoDI GTCTC 2 cut(s) 155, 240
BcuI ACTAGT 1 cut(s) 632
BfaI CTAG 1 cut(s) 633
BfuI GTATCC 1 cut(s) 241
BglI GCCNNNNNGGC 1 cut(s) 11
BisI GCNGC 5 cut(s) 6, 80, 101, 117, 598
BlsI GCNGC 5 cut(s) 7, 81, 102, 118, 599
Bme1390I CCNGG 3 cut(s) 132, 393, 608
Bme18I GGWCC 2 cut(s) 395, 610
BmgT120I GGNCC 2 cut(s) 395, 610
BmiI GGNNCC 1 cut(s) 390
BmrFI CCNGG 3 cut(s) 132, 393, 608
BpmI CTGGAG 1 cut(s) 375
BpuEI CTTGAG 1 cut(s) 202
BsaJI CCNNGG 1 cut(s) 130
Bsc4I CCNNNNNNNGG 1 cut(s) 32
Bse118I RCCGGY 1 cut(s) 10
Bse1I ACTGG 2 cut(s) 37, 215
BseBI CCWGG 3 cut(s) 132, 393, 608
BseDI CCNNGG 1 cut(s) 130
BseGI GGATG 3 cut(s) 71, 140, 445
BseLI CCNNNNNNNGG 1 cut(s) 32
BseMII CTCAG 1 cut(s) 78
BseNI ACTGG 2 cut(s) 37, 215
BseXI GCAGC 2 cut(s) 87, 609
Bsh1236I CGCG 2 cut(s) 8, 116
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 53
BsiSI CCGG 1 cut(s) 11
BslFI GGGAC 1 cut(s) 166
BslI CCNNNNNNNGG 1 cut(s) 32
BsmAI GTCTC 2 cut(s) 155, 240
BsmFI GGGAC 1 cut(s) 166
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 53
Bsp143I GATC 1 cut(s) 95
BspACI CCGC 5 cut(s) 6, 41, 77, 80, 116
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 79
BspFNI CGCG 2 cut(s) 8, 116
BspLI GGNNCC 1 cut(s) 390
BspQI GCTCTTC 1 cut(s) 352
BsrFI RCCGGY 1 cut(s) 10
BsrI ACTGG 2 cut(s) 37, 215
BssAI RCCGGY 1 cut(s) 10
BssECI CCNNGG 1 cut(s) 130
BssMI GATC 1 cut(s) 95
Bst2UI CCWGG 3 cut(s) 132, 393, 608
Bst4CI ACNGT 2 cut(s) 318, 543
Bst6I CTCTTC 4 cut(s) 352, 471, 513, 630
BstC8I GCNNGC 4 cut(s) 12, 112, 330, 555
BstDEI CTNAG 3 cut(s) 87, 360, 380
BstF5I GGATG 3 cut(s) 71, 140, 445
BstFNI CGCG 2 cut(s) 8, 116
BstHHI GCGC 2 cut(s) 10, 116
BstKTI GATC 1 cut(s) 98
BstMAI GTCTC 2 cut(s) 155, 240
BstMBI GATC 1 cut(s) 95
BstMWI GCNNNNNNNGC 4 cut(s) 11, 116, 125, 370
BstNI CCWGG 3 cut(s) 132, 393, 608
BstSCI CCNGG 3 cut(s) 130, 391, 606
BstUI CGCG 2 cut(s) 8, 116
BstV1I GCAGC 2 cut(s) 87, 609
BsuI GTATCC 1 cut(s) 241
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 3 cut(s) 71, 140, 445
Cac8I GCNNGC 4 cut(s) 12, 112, 330, 555
CaiI CAGNNNCTG 1 cut(s) 398
CfoI GCGC 2 cut(s) 10, 116
Cfr10I RCCGGY 1 cut(s) 10
Cfr13I GGNCC 2 cut(s) 395, 610
Csp6I GTAC 1 cut(s) 144
CviAII CATG 2 cut(s) 448, 590
CviQI GTAC 1 cut(s) 144
DdeI CTNAG 3 cut(s) 87, 360, 380
DpnI GATC 1 cut(s) 97
DpnII GATC 1 cut(s) 95
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 4 cut(s) 352, 471, 513, 630
EarI CTCTTC 4 cut(s) 352, 471, 513, 630
Eco47I GGWCC 2 cut(s) 395, 610
EcoO109I RGGNCCY 2 cut(s) 395, 610
EcoRII CCWGG 3 cut(s) 130, 391, 606
FaeI CATG 2 cut(s) 451, 593
FaiI YATR 8 cut(s) 201, 225, 231, 449, 550, 562, 591, 647
FaqI GGGAC 1 cut(s) 166
FatI CATG 2 cut(s) 447, 589
FauI CCCGC 1 cut(s) 84
Fnu4HI GCNGC 5 cut(s) 6, 80, 101, 117, 598
FokI GGATG 3 cut(s) 58, 147, 452
Fsp4HI GCNGC 5 cut(s) 6, 80, 101, 117, 598
FspBI CTAG 1 cut(s) 633
GlaI GCGC 2 cut(s) 9, 115
GluI GCNGC 5 cut(s) 6, 80, 101, 117, 598
GsuI CTGGAG 1 cut(s) 375
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 11
HhaI GCGC 2 cut(s) 10, 116
Hin1II CATG 2 cut(s) 451, 593
Hin6I GCGC 2 cut(s) 8, 114
HinP1I GCGC 2 cut(s) 8, 114
HinfI GANTC 9 cut(s) 89, 148, 170, 233, 269, 376, 404, 444, 619
HpaII CCGG 1 cut(s) 11
HphI GGTGA 3 cut(s) 30, 317, 527
Hpy188I TCNGA 3 cut(s) 169, 190, 424
Hpy188III TCNNGA 4 cut(s) 93, 321, 351, 657
HpyAV CCTTC 2 cut(s) 36, 249
HpyCH4III ACNGT 2 cut(s) 318, 543
HpyCH4V TGCA 3 cut(s) 279, 332, 557
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 116, 125, 370
HpyF3I CTNAG 3 cut(s) 87, 360, 380
Hsp92II CATG 2 cut(s) 451, 593
HspAI GCGC 2 cut(s) 8, 114
KroI GCCGGC 1 cut(s) 10
KroNI GCCGGC 1 cut(s) 12
Kzo9I GATC 1 cut(s) 95
LguI GCTCTTC 1 cut(s) 352
LmnI GCTCC 1 cut(s) 394
Lsp1109I GCAGC 2 cut(s) 87, 609
MaeI CTAG 1 cut(s) 633
MaeIII GTNAC 4 cut(s) 171, 338, 563, 650
MalI GATC 1 cut(s) 97
MboI GATC 1 cut(s) 95
MboII GAAGA 8 cut(s) 156, 322, 339, 488, 510, 530, 547, 617
MhlI GDGCHC 1 cut(s) 53
MluCI AATT 2 cut(s) 288, 334
MlyI GAGTC 5 cut(s) 98, 157, 179, 242, 370
MnlI CCTC 7 cut(s) 76, 289, 317, 379, 472, 514, 633
MroNI GCCGGC 1 cut(s) 10
MroXI GAANNNNTTC 1 cut(s) 25
MspI CCGG 1 cut(s) 11
MspR9I CCNGG 3 cut(s) 132, 393, 608
MvaI CCWGG 3 cut(s) 132, 393, 608
MvnI CGCG 2 cut(s) 8, 116
MwoI GCNNNNNNNGC 4 cut(s) 11, 116, 125, 370
NaeI GCCGGC 1 cut(s) 12
NdeII GATC 1 cut(s) 95
NgoMIV GCCGGC 1 cut(s) 10
NlaIII CATG 2 cut(s) 451, 593
NlaIV GGNNCC 1 cut(s) 390
NmuCI GTSAC 3 cut(s) 171, 338, 563
PciSI GCTCTTC 1 cut(s) 352
PcsI WCGNNNNNNNCGW 1 cut(s) 65
PdiI GCCGGC 1 cut(s) 12
PdmI GAANNNNTTC 1 cut(s) 25
PfeI GAWTC 4 cut(s) 269, 404, 444, 619
PflFI GACNNNGTC 1 cut(s) 88
PkrI GCNGC 5 cut(s) 7, 81, 102, 118, 599
PleI GAGTC 5 cut(s) 97, 156, 178, 241, 370
PpsI GAGTC 5 cut(s) 97, 156, 178, 241, 370
PpuMI RGGWCCY 2 cut(s) 395, 610
Psp5II RGGWCCY 2 cut(s) 395, 610
Psp6I CCWGG 3 cut(s) 130, 391, 606
PspGI CCWGG 3 cut(s) 130, 391, 606
PspN4I GGNNCC 1 cut(s) 390
PspPI GGNCC 2 cut(s) 395, 610
PspPPI RGGWCCY 2 cut(s) 395, 610
PsrI GAACNNNNNNTAC 2 cut(s) 13, 45
PstNI CAGNNNCTG 1 cut(s) 398
PsyI GACNNNGTC 1 cut(s) 88
RsaI GTAC 1 cut(s) 145
RsaNI GTAC 1 cut(s) 144
SapI GCTCTTC 1 cut(s) 352
SatI GCNGC 5 cut(s) 6, 80, 101, 117, 598
Sau3AI GATC 1 cut(s) 95
Sau96I GGNCC 2 cut(s) 395, 610
SchI GAGTC 5 cut(s) 98, 157, 179, 242, 370
ScrFI CCNGG 3 cut(s) 132, 393, 608
SduI GDGCHC 1 cut(s) 53
SinI GGWCC 2 cut(s) 395, 610
SmlI CTYRAG 1 cut(s) 217
SmoI CTYRAG 1 cut(s) 217
SpeI ACTAGT 1 cut(s) 632
Sse9I AATT 2 cut(s) 288, 334
SsiI CCGC 5 cut(s) 6, 41, 77, 80, 116
SspMI CTAG 1 cut(s) 633
StyD4I CCNGG 3 cut(s) 130, 391, 606
TaaI ACNGT 2 cut(s) 318, 543
TaqI TCGA 2 cut(s) 68, 350
TasI AATT 2 cut(s) 288, 334
TauI GCSGC 3 cut(s) 8, 82, 119
TfiI GAWTC 4 cut(s) 269, 404, 444, 619
TseFI GTSAC 3 cut(s) 171, 338, 563
TseI GCWGC 2 cut(s) 100, 597
Tsp45I GTSAC 3 cut(s) 171, 338, 563
TspDTI ATGAA 3 cut(s) 231, 436, 489
Tth111I GACNNNGTC 1 cut(s) 88
VpaK11BI GGWCC 2 cut(s) 395, 610
XapI RAATTY 1 cut(s) 288
XmnI GAANNNNTTC 1 cut(s) 25
XspI CTAG 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.