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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 4 cut(s) | 173, 190, 256, 308 |
| AclWI | GGATC | 1 cut(s) | 160 |
| AcsI | RAATTY | 1 cut(s) | 71 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 38, 89, 196 |
| AgsI | TTSAA | 1 cut(s) | 71 |
| Alw21I | GWGCWC | 2 cut(s) | 31, 269 |
| Alw26I | GTCTC | 1 cut(s) | 21 |
| AlwI | GGATC | 1 cut(s) | 160 |
| ApeKI | GCWGC | 2 cut(s) | 42, 217 |
| ApoI | RAATTY | 1 cut(s) | 71 |
| AsuHPI | GGTGA | 2 cut(s) | 104, 313 |
| Bbv12I | GWGCWC | 2 cut(s) | 31, 269 |
| BbvI | GCAGC | 2 cut(s) | 54, 204 |
| BccI | CCATC | 2 cut(s) | 89, 160 |
| BceAI | ACGGC | 1 cut(s) | 195 |
| BcoDI | GTCTC | 1 cut(s) | 21 |
| BfaI | CTAG | 2 cut(s) | 20, 117 |
| BisI | GCNGC | 4 cut(s) | 43, 174, 218, 309 |
| BlsI | GCNGC | 4 cut(s) | 44, 175, 219, 310 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 38, 89, 196 |
| Bse1I | ACTGG | 1 cut(s) | 277 |
| Bse3DI | GCAATG | 1 cut(s) | 251 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 38, 89, 196 |
| BseMI | GCAATG | 1 cut(s) | 251 |
| BseNI | ACTGG | 1 cut(s) | 277 |
| BseRI | GAGGAG | 1 cut(s) | 21 |
| BseXI | GCAGC | 2 cut(s) | 54, 204 |
| BseYI | CCCAGC | 1 cut(s) | 99 |
| BsiHKAI | GWGCWC | 2 cut(s) | 31, 269 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 38, 89, 196 |
| BsmAI | GTCTC | 1 cut(s) | 21 |
| Bsp1286I | GDGCHC | 2 cut(s) | 31, 269 |
| Bsp143I | GATC | 2 cut(s) | 139, 152 |
| BspACI | CCGC | 4 cut(s) | 173, 190, 256, 308 |
| BspPI | GGATC | 1 cut(s) | 160 |
| BsrDI | GCAATG | 1 cut(s) | 251 |
| BsrI | ACTGG | 1 cut(s) | 277 |
| BssMI | GATC | 2 cut(s) | 139, 152 |
| BstC8I | GCNNGC | 3 cut(s) | 11, 194, 198 |
| BstKTI | GATC | 2 cut(s) | 142, 155 |
| BstMAI | GTCTC | 1 cut(s) | 21 |
| BstMBI | GATC | 2 cut(s) | 139, 152 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 179, 226, 253 |
| BstV1I | GCAGC | 2 cut(s) | 54, 204 |
| BstX2I | RGATCY | 1 cut(s) | 152 |
| BstYI | RGATCY | 1 cut(s) | 152 |
| BtsI | GCAGTG | 1 cut(s) | 52 |
| BtsIMutI | CAGTG | 1 cut(s) | 52 |
| Cac8I | GCNNGC | 3 cut(s) | 11, 194, 198 |
| CseI | GACGC | 1 cut(s) | 122 |
| CviJI | RGCY | 7 cut(s) | 9, 58, 99, 196, 200, 217, 247 |
| CviKI_1 | RGCY | 7 cut(s) | 9, 58, 99, 196, 200, 217, 247 |
| DpnI | GATC | 2 cut(s) | 141, 154 |
| DpnII | GATC | 2 cut(s) | 139, 152 |
| EcoRI | GAATTC | 1 cut(s) | 71 |
| Fnu4HI | GCNGC | 4 cut(s) | 43, 174, 218, 309 |
| Fsp4HI | GCNGC | 4 cut(s) | 43, 174, 218, 309 |
| FspBI | CTAG | 2 cut(s) | 20, 117 |
| GluI | GCNGC | 4 cut(s) | 43, 174, 218, 309 |
| GsaI | CCCAGC | 1 cut(s) | 103 |
| HgaI | GACGC | 1 cut(s) | 122 |
| HphI | GGTGA | 2 cut(s) | 104, 313 |
| Hpy166II | GTNNAC | 1 cut(s) | 278 |
| Hpy188I | TCNGA | 1 cut(s) | 157 |
| Hpy188III | TCNNGA | 1 cut(s) | 143 |
| Hpy8I | GTNNAC | 1 cut(s) | 278 |
| Hpy99I | CGWCG | 2 cut(s) | 181, 293 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 179, 226, 253 |
| Kzo9I | GATC | 2 cut(s) | 139, 152 |
| LmnI | GCTCC | 2 cut(s) | 34, 264 |
| LpnPI | CCDG | 5 cut(s) | 85, 128, 178, 182, 258 |
| Lsp1109I | GCAGC | 2 cut(s) | 54, 204 |
| MaeI | CTAG | 2 cut(s) | 20, 117 |
| MalI | GATC | 2 cut(s) | 141, 154 |
| MboI | GATC | 2 cut(s) | 139, 152 |
| MflI | RGATCY | 1 cut(s) | 152 |
| MhlI | GDGCHC | 2 cut(s) | 31, 269 |
| MluCI | AATT | 2 cut(s) | 71, 129 |
| MmeI | TCCRAC | 1 cut(s) | 130 |
| MnlI | CCTC | 3 cut(s) | 42, 82, 139 |
| MseI | TTAA | 3 cut(s) | 62, 107, 203 |
| MslI | CAYNNNNRTG | 1 cut(s) | 256 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 179, 226, 253 |
| NdeII | GATC | 2 cut(s) | 139, 152 |
| PkrI | GCNGC | 4 cut(s) | 44, 175, 219, 310 |
| PspFI | CCCAGC | 1 cut(s) | 99 |
| PsuI | RGATCY | 1 cut(s) | 152 |
| RseI | CAYNNNNRTG | 1 cut(s) | 256 |
| SaqAI | TTAA | 3 cut(s) | 62, 107, 203 |
| SatI | GCNGC | 4 cut(s) | 43, 174, 218, 309 |
| Sau3AI | GATC | 2 cut(s) | 139, 152 |
| SduI | GDGCHC | 2 cut(s) | 31, 269 |
| SetI | ASST | 2 cut(s) | 150, 326 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 256 |
| Sse9I | AATT | 2 cut(s) | 71, 129 |
| SsiI | CCGC | 4 cut(s) | 173, 190, 256, 308 |
| SspMI | CTAG | 2 cut(s) | 20, 117 |
| TasI | AATT | 2 cut(s) | 71, 129 |
| TauI | GCSGC | 2 cut(s) | 176, 311 |
| Tru1I | TTAA | 3 cut(s) | 62, 107, 203 |
| Tru9I | TTAA | 3 cut(s) | 62, 107, 203 |
| TscAI | CASTG | 1 cut(s) | 52 |
| TseI | GCWGC | 2 cut(s) | 42, 217 |
| TspDTI | ATGAA | 1 cut(s) | 64 |
| TspGWI | ACGGA | 1 cut(s) | 295 |
| TspRI | CASTG | 1 cut(s) | 52 |
| XapI | RAATTY | 1 cut(s) | 71 |
| XspI | CTAG | 2 cut(s) | 20, 117 |