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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AflIII | ACRYGT | 1 cut(s) | 113 |
| AgsI | TTSAA | 3 cut(s) | 8, 193, 263 |
| AjnI | CCWGG | 1 cut(s) | 125 |
| AluBI | AGCT | 2 cut(s) | 123, 200 |
| AluI | AGCT | 2 cut(s) | 123, 200 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 126, 176 |
| ApeKI | GCWGC | 1 cut(s) | 151 |
| AsuHPI | GGTGA | 1 cut(s) | 80 |
| BbvI | GCAGC | 1 cut(s) | 163 |
| BciT130I | CCWGG | 1 cut(s) | 127 |
| BfaI | CTAG | 1 cut(s) | 156 |
| BfmI | CTRYAG | 1 cut(s) | 382 |
| BisI | GCNGC | 1 cut(s) | 152 |
| BlsI | GCNGC | 1 cut(s) | 153 |
| Bme1390I | CCNGG | 1 cut(s) | 127 |
| BmrFI | CCNGG | 1 cut(s) | 127 |
| BmsI | GCATC | 1 cut(s) | 193 |
| BpmI | CTGGAG | 1 cut(s) | 148 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 204, 234 |
| Bse1I | ACTGG | 1 cut(s) | 181 |
| BseBI | CCWGG | 1 cut(s) | 127 |
| BseGI | GGATG | 1 cut(s) | 253 |
| BseNI | ACTGG | 1 cut(s) | 181 |
| BseRI | GAGGAG | 1 cut(s) | 86 |
| BseXI | GCAGC | 1 cut(s) | 163 |
| BseYI | CCCAGC | 1 cut(s) | 266 |
| BspMAI | CTGCAG | 1 cut(s) | 386 |
| BsrI | ACTGG | 1 cut(s) | 181 |
| Bst2UI | CCWGG | 1 cut(s) | 127 |
| Bst4CI | ACNGT | 2 cut(s) | 96, 351 |
| Bst6I | CTCTTC | 4 cut(s) | 24, 273, 321, 351 |
| BstC8I | GCNNGC | 1 cut(s) | 108 |
| BstF5I | GGATG | 1 cut(s) | 253 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 148 |
| BstNI | CCWGG | 1 cut(s) | 127 |
| BstNSI | RCATGY | 1 cut(s) | 117 |
| BstSCI | CCNGG | 1 cut(s) | 125 |
| BstSFI | CTRYAG | 1 cut(s) | 382 |
| BstV1I | GCAGC | 1 cut(s) | 163 |
| BtsCI | GGATG | 1 cut(s) | 253 |
| Cac8I | GCNNGC | 1 cut(s) | 108 |
| CaiI | CAGNNNCTG | 2 cut(s) | 126, 176 |
| CviAII | CATG | 2 cut(s) | 114, 143 |
| CviJI | RGCY | 5 cut(s) | 106, 123, 154, 159, 200 |
| CviKI_1 | RGCY | 5 cut(s) | 106, 123, 154, 159, 200 |
| Eam1104I | CTCTTC | 4 cut(s) | 24, 273, 321, 351 |
| EarI | CTCTTC | 4 cut(s) | 24, 273, 321, 351 |
| EcoRII | CCWGG | 1 cut(s) | 125 |
| FaeI | CATG | 2 cut(s) | 117, 146 |
| FaiI | YATR | 4 cut(s) | 115, 144, 372, 390 |
| FatI | CATG | 2 cut(s) | 113, 142 |
| Fnu4HI | GCNGC | 1 cut(s) | 152 |
| FokI | GGATG | 1 cut(s) | 260 |
| Fsp4HI | GCNGC | 1 cut(s) | 152 |
| FspBI | CTAG | 1 cut(s) | 156 |
| GluI | GCNGC | 1 cut(s) | 152 |
| GsaI | CCCAGC | 1 cut(s) | 270 |
| GsuI | CTGGAG | 1 cut(s) | 148 |
| Hin1II | CATG | 2 cut(s) | 117, 146 |
| HinfI | GANTC | 2 cut(s) | 58, 134 |
| HphI | GGTGA | 1 cut(s) | 80 |
| Hpy188I | TCNGA | 3 cut(s) | 172, 355, 365 |
| HpyAV | CCTTC | 1 cut(s) | 238 |
| HpyCH4III | ACNGT | 2 cut(s) | 96, 351 |
| HpyCH4V | TGCA | 3 cut(s) | 80, 110, 384 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 148 |
| Hsp92II | CATG | 2 cut(s) | 117, 146 |
| LmnI | GCTCC | 1 cut(s) | 128 |
| LpnPI | CCDG | 6 cut(s) | 66, 112, 139, 162, 280, 394 |
| Lsp1109I | GCAGC | 1 cut(s) | 163 |
| LweI | GCATC | 1 cut(s) | 193 |
| MaeI | CTAG | 1 cut(s) | 156 |
| MaeIII | GTNAC | 1 cut(s) | 116 |
| MboII | GAAGA | 7 cut(s) | 41, 63, 100, 290, 338, 355, 368 |
| MnlI | CCTC | 8 cut(s) | 25, 64, 67, 274, 280, 289, 322, 388 |
| MseI | TTAA | 2 cut(s) | 402, 409 |
| MspA1I | CMGCKG | 1 cut(s) | 200 |
| MspR9I | CCNGG | 1 cut(s) | 127 |
| MvaI | CCWGG | 1 cut(s) | 127 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 148 |
| NlaIII | CATG | 2 cut(s) | 117, 146 |
| NmuCI | GTSAC | 1 cut(s) | 116 |
| NspI | RCATGY | 1 cut(s) | 117 |
| PciI | ACATGT | 1 cut(s) | 113 |
| PfeI | GAWTC | 2 cut(s) | 58, 134 |
| PfoI | TCCNGGA | 1 cut(s) | 125 |
| PkrI | GCNGC | 1 cut(s) | 153 |
| PscI | ACATGT | 1 cut(s) | 113 |
| Psp6I | CCWGG | 1 cut(s) | 125 |
| PspFI | CCCAGC | 1 cut(s) | 266 |
| PspGI | CCWGG | 1 cut(s) | 125 |
| PstI | CTGCAG | 1 cut(s) | 386 |
| PstNI | CAGNNNCTG | 2 cut(s) | 126, 176 |
| PvuII | CAGCTG | 1 cut(s) | 200 |
| SaqAI | TTAA | 2 cut(s) | 402, 409 |
| SatI | GCNGC | 1 cut(s) | 152 |
| ScrFI | CCNGG | 1 cut(s) | 127 |
| SetI | ASST | 5 cut(s) | 78, 125, 202, 333, 380 |
| SfaNI | GCATC | 1 cut(s) | 193 |
| SfcI | CTRYAG | 1 cut(s) | 382 |
| SspMI | CTAG | 1 cut(s) | 156 |
| StyD4I | CCNGG | 1 cut(s) | 125 |
| TaaI | ACNGT | 2 cut(s) | 96, 351 |
| TfiI | GAWTC | 2 cut(s) | 58, 134 |
| Tru1I | TTAA | 2 cut(s) | 402, 409 |
| Tru9I | TTAA | 2 cut(s) | 402, 409 |
| TseFI | GTSAC | 1 cut(s) | 116 |
| TseI | GCWGC | 1 cut(s) | 151 |
| Tsp45I | GTSAC | 1 cut(s) | 116 |
| TspDTI | ATGAA | 2 cut(s) | 42, 264 |
| XceI | RCATGY | 1 cut(s) | 117 |
| XspI | CTAG | 1 cut(s) | 156 |