Rmu_sc0003221.1_g000060

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003221.1
OG0001095
Physical Location & Seq
Forward (+)
283029 .. 287016
3988 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003221.1_g000060.1.cds

Sequence Viewer

Length: 1812 bp
atgtcagaacctcctgacctgggaaattggttctcaagctaccgctatgaatcacctgcaatgaattccaattcttttcgcaatgaatttgataccggcgagatcaaaagagaaaaagatggaggagattcatgtgggtttatggaaaatggaaataaagttggagtctttgttcataagaagcggaactcaaatggggttgtcaaatgtggcagctcttcacaaaaccaatgcttaaagcctacgcacatcaaaaactggttctcaagctatgtgtatgagtctccttcattggatacaactgctggttttggcgattctctttgtaaagaaagtaaatttgagaaggatgggtttttggttgaaaacagcaacagagaaaatgaagaggatttgaggaattttagtacaaacagcagtgttagtaaggagattgtgggtgagaaggaacagtcaggagagcttgcaaattgtgacagctcttggagagatactaagcaggcaaggcagcctgctccaggtcctctggaatcacatttgctcctttcagaacctattcacatcagaaactggttctcaagctatgtgtatgagtctccttccttggatatgaatgatagttttggagattctgtttgcaaagaaagtaaatgtgagaaggatggatttagggttgaaaagagagataatgaagaggatttgggggattttagaagaaacagtggtgttggtgtaggggttgcgggagagaagaaacagtcagtagggcttgcaacatgtgacagctcttggagagttaccaagcatgataggcagcctgtaagtaaggttccgggtcctttggattccacttcacacttgtcagagcctactgacatcagaaactggttctcaagctatgtgtatgagtctcctttggatacagctgatggttttggagattttggttgtagagatagaatagatgagaaggatgacgttttggttgaacccagtaacagagaagacggagagggtttggagggttttagcaaaaaggaaagtgatagtgaagaggttgatgttgagaaggtacagtcagaagagttcgcaaaatataacacctcttgcagatgtactgtggttgttgacccaaccactggagtagatgaaaatctatctggtaggaatgatttgtgtcttgaatttacacctaaaacagtgcaaaaccatgataccagtcaagcaaaacatggtgatacatcaagcttcaacaaaggagattctcaatgcaagcgagatcaagagactttccatggaaccagatttaagccatcgaacaaacagagaagtatgtgcagtaatgatggcatatgtccacaaaacttgattcagagtagtggttccactttacaggagagctcagaagcaacggtccatgcagaagattgtgtacaattcaaaagtgattcagagttaattccagttaaaggagcatcggtaagcaaatcaactcatggaagcaaagaaagaaaggatggtggaaaaggaatatcagaagatggtttcattacaacaaggaaacgcaaatttacagcgagaaatgatgaaaactcttcaaaaagacctgcagagagtagtaagggaacagttccattagtaggcagaaaggatgttgctattaaaagaaaggctttagcagacacaacaaattttcatcattctccggcaacgatgatcactgggaaatggaagcgccctcagcaacgtaagccaaatttaagacctcctttgaagcagcttggacttgagcaatggattcagaagttgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

603

Amino Acids

67.19

Weight (kDa)

5.71

Isoelectric Point (pI)

49.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 64
Acc36I ACCTGC 2 cut(s) 64, 1615
AccB7I CCANNNNNTGG 1 cut(s) 1139
AciI CCGC 3 cut(s) 43, 184, 743
AcsI RAATTY 8 cut(s) 64, 86, 338, 400, 1184, 1568, 1690, 1756
AfaI GTAC 4 cut(s) 409, 1074, 1117, 1434
AfiI CCNNNNNNNGG 5 cut(s) 20, 518, 1139, 1469, 1640
AflIII ACRYGT 1 cut(s) 776
AgsI TTSAA 8 cut(s) 365, 677, 989, 1184, 1252, 1441, 1599, 1774
AjnI CCWGG 2 cut(s) 18, 517
AloI GAACNNNNNNTCC 2 cut(s) 156, 188
Alw21I GWGCWC 1 cut(s) 1403
Alw26I GTCTC 4 cut(s) 288, 600, 915, 1280
AlwNI CAGNNNCTG 2 cut(s) 570, 885
ApeKI GCWGC 4 cut(s) 213, 508, 814, 1777
ApoI RAATTY 8 cut(s) 64, 86, 338, 400, 1184, 1568, 1690, 1756
Asp700I GAANNNNTTC 1 cut(s) 555
AspLEI GCGC 1 cut(s) 1737
AspS9I GGNCC 3 cut(s) 521, 836, 1414
AsuC2I CCSGG 1 cut(s) 834
AsuHPI GGTGA 3 cut(s) 45, 452, 1247
AvaII GGWCC 3 cut(s) 521, 836, 1414
BanII GRGCYC 1 cut(s) 1403
BarI GAAGNNNNNNTAC 2 cut(s) 1416, 1448
BbsI GAAGAC 1 cut(s) 1011
Bbv12I GWGCWC 1 cut(s) 1403
BbvCI CCTCAGC 1 cut(s) 1740
BbvI GCAGC 4 cut(s) 225, 520, 826, 1789
BccI CCATC 8 cut(s) 113, 344, 656, 923, 1321, 1340, 1511, 1535
BciT130I CCWGG 2 cut(s) 20, 519
BciVI GTATCC 2 cut(s) 289, 913
BclI TGATCA 1 cut(s) 1716
BcnI CCSGG 1 cut(s) 834
BcoDI GTCTC 4 cut(s) 288, 600, 915, 1280
BfmI CTRYAG 1 cut(s) 1608
BfoI RGCGCY 1 cut(s) 1738
BfuAI ACCTGC 2 cut(s) 64, 1615
BfuI GTATCC 2 cut(s) 289, 913
BisI GCNGC 4 cut(s) 214, 509, 815, 1778
BlsI GCNGC 4 cut(s) 215, 510, 816, 1779
Bme1390I CCNGG 3 cut(s) 20, 519, 834
Bme18I GGWCC 3 cut(s) 521, 836, 1414
BmgT120I GGNCC 3 cut(s) 521, 836, 1414
BmiI GGNNCC 4 cut(s) 831, 837, 1300, 1384
BmrFI CCNGG 3 cut(s) 20, 519, 834
BmrI ACTGGG 2 cut(s) 987, 1731
BmsI GCATC 1 cut(s) 1484
BmuI ACTGGG 2 cut(s) 987, 1731
BpiI GAAGAC 1 cut(s) 1011
BpmI CTGGAG 2 cut(s) 501, 1161
Bpu10I CCTNAGC 1 cut(s) 1740
BpuEI CTTGAG 5 cut(s) 19, 250, 562, 877, 1808
BpuMI CCSGG 1 cut(s) 834
BsaBI GATNNNNATC 1 cut(s) 1152
BsaJI CCNNGG 3 cut(s) 19, 603, 1294
BsaXI ACNNNNNCTCC 8 cut(s) 156, 186, 525, 555, 618, 648, 1464, 1494
Bsc4I CCNNNNNNNGG 5 cut(s) 20, 518, 1139, 1469, 1640
Bse118I RCCGGY 1 cut(s) 95
Bse1I ACTGG 8 cut(s) 263, 575, 890, 993, 1144, 1218, 1463, 1726
Bse3DI GCAATG 3 cut(s) 66, 88, 1799
Bse8I GATNNNNATC 1 cut(s) 1152
BseBI CCWGG 2 cut(s) 20, 519
BseDI CCNNGG 3 cut(s) 19, 603, 1294
BseGI GGATG 5 cut(s) 355, 667, 979, 1522, 1657
BseJI GATNNNNATC 1 cut(s) 1152
BseLI CCNNNNNNNGG 5 cut(s) 20, 518, 1139, 1469, 1640
BseMI GCAATG 3 cut(s) 66, 88, 1799
BseMII CTCAG 2 cut(s) 1416, 1754
BseNI ACTGG 8 cut(s) 263, 575, 890, 993, 1144, 1218, 1463, 1726
BseRI GAGGAG 1 cut(s) 138
BseXI GCAGC 4 cut(s) 225, 520, 826, 1789
BsgI GTGCAG 1 cut(s) 1357
BsiHKAI GWGCWC 1 cut(s) 1403
BsiSI CCGG 3 cut(s) 96, 833, 1706
BslI CCNNNNNNNGG 5 cut(s) 20, 518, 1139, 1469, 1640
BsmAI GTCTC 4 cut(s) 288, 600, 915, 1280
Bsp1286I GDGCHC 1 cut(s) 1403
Bsp1407I TGTACA 1 cut(s) 1432
Bsp143I GATC 3 cut(s) 102, 1279, 1716
Bsp19I CCATGG 1 cut(s) 1294
BspACI CCGC 3 cut(s) 43, 184, 743
BspCNI CTCAG 2 cut(s) 1415, 1753
BspLI GGNNCC 4 cut(s) 831, 837, 1300, 1384
BspMAI CTGCAG 1 cut(s) 1612
BspMI ACCTGC 2 cut(s) 64, 1615
BspQI GCTCTTC 1 cut(s) 223
BsrDI GCAATG 3 cut(s) 66, 88, 1799
BsrFI RCCGGY 1 cut(s) 95
BsrGI TGTACA 1 cut(s) 1432
BsrI ACTGG 8 cut(s) 263, 575, 890, 993, 1144, 1218, 1463, 1726
BssAI RCCGGY 1 cut(s) 95
BssECI CCNNGG 3 cut(s) 19, 603, 1294
BssMI GATC 3 cut(s) 102, 1279, 1716
BssT1I CCWWGG 2 cut(s) 603, 1294
Bst2UI CCWGG 2 cut(s) 20, 519
Bst4CI ACNGT 8 cut(s) 453, 722, 759, 1077, 1120, 1201, 1414, 1630
Bst6I CTCTTC 6 cut(s) 223, 381, 687, 1047, 1077, 1600
BstAUI TGTACA 1 cut(s) 1432
BstC8I GCNNGC 5 cut(s) 465, 501, 513, 771, 1274
BstDEI CTNAG 3 cut(s) 495, 1402, 1740
BstDSI CCRYGG 1 cut(s) 1294
BstENI CCTNNNNNAGG 1 cut(s) 516
BstF5I GGATG 5 cut(s) 355, 667, 979, 1522, 1657
BstH2I RGCGCY 1 cut(s) 1738
BstHHI GCGC 1 cut(s) 1737
BstKTI GATC 3 cut(s) 105, 1282, 1719
BstMAI GTCTC 4 cut(s) 288, 600, 915, 1280
BstMBI GATC 3 cut(s) 102, 1279, 1716
BstMWI GCNNNNNNNGC 4 cut(s) 505, 811, 1741, 1750
BstNI CCWGG 2 cut(s) 20, 519
BstNSI RCATGY 1 cut(s) 780
BstSCI CCNGG 3 cut(s) 18, 517, 832
BstSFI CTRYAG 1 cut(s) 1608
BstV1I GCAGC 4 cut(s) 225, 520, 826, 1789
BstV2I GAAGAC 1 cut(s) 1011
BsuI GTATCC 2 cut(s) 289, 913
BtgI CCRYGG 1 cut(s) 1294
BtsCI GGATG 5 cut(s) 355, 667, 979, 1522, 1657
BtsI GCAGTG 1 cut(s) 424
BtsIMutI CAGTG 5 cut(s) 424, 727, 1137, 1206, 1719
BveI ACCTGC 2 cut(s) 64, 1615
Cac8I GCNNGC 5 cut(s) 465, 501, 513, 771, 1274
CaiI CAGNNNCTG 2 cut(s) 570, 885
CfoI GCGC 1 cut(s) 1737
Cfr10I RCCGGY 1 cut(s) 95
Cfr13I GGNCC 3 cut(s) 521, 836, 1414
Csp6I GTAC 4 cut(s) 408, 1073, 1116, 1433
CspCI CAANNNNNGTGG 2 cut(s) 1347, 1382
CviAII CATG 8 cut(s) 132, 777, 806, 1211, 1232, 1295, 1418, 1496
CviQI GTAC 4 cut(s) 408, 1073, 1116, 1433
DdeI CTNAG 3 cut(s) 495, 1402, 1740
DpnI GATC 3 cut(s) 104, 1281, 1718
DpnII GATC 3 cut(s) 102, 1279, 1716
Eam1104I CTCTTC 6 cut(s) 223, 381, 687, 1047, 1077, 1600
EarI CTCTTC 6 cut(s) 223, 381, 687, 1047, 1077, 1600
Ecl136II GAGCTC 1 cut(s) 1401
Eco130I CCWWGG 2 cut(s) 603, 1294
Eco24I GRGCYC 1 cut(s) 1403
Eco47I GGWCC 3 cut(s) 521, 836, 1414
Eco53kI GAGCTC 1 cut(s) 1401
EcoICRI GAGCTC 1 cut(s) 1401
EcoNI CCTNNNNNAGG 1 cut(s) 516
EcoO109I RGGNCCY 2 cut(s) 521, 836
EcoRI GAATTC 1 cut(s) 64
EcoRII CCWGG 2 cut(s) 18, 517
EcoT14I CCWWGG 2 cut(s) 603, 1294
EcoT38I GRGCYC 1 cut(s) 1403
ErhI CCWWGG 2 cut(s) 603, 1294
FaeI CATG 8 cut(s) 135, 780, 809, 1214, 1235, 1298, 1421, 1499
FalI AAGNNNNNCTT 4 cut(s) 1657, 1689, 1753, 1785
FatI CATG 8 cut(s) 131, 776, 805, 1210, 1231, 1294, 1417, 1495
FauI CCCGC 1 cut(s) 736
FauNDI CATATG 1 cut(s) 1352
FbaI TGATCA 1 cut(s) 1716
Fnu4HI GCNGC 4 cut(s) 214, 509, 815, 1778
FokI GGATG 5 cut(s) 362, 674, 986, 1529, 1664
FriOI GRGCYC 1 cut(s) 1403
Fsp4HI GCNGC 4 cut(s) 214, 509, 815, 1778
GlaI GCGC 1 cut(s) 1736
GluI GCNGC 4 cut(s) 214, 509, 815, 1778
GsuI CTGGAG 2 cut(s) 501, 1161
HaeII RGCGCY 1 cut(s) 1738
HapII CCGG 3 cut(s) 96, 833, 1706
HhaI GCGC 1 cut(s) 1737
Hin1II CATG 8 cut(s) 135, 780, 809, 1214, 1235, 1298, 1421, 1499
Hin6I GCGC 1 cut(s) 1735
HinP1I GCGC 1 cut(s) 1735
HincII GTYRAC 1 cut(s) 1129
HindII GTYRAC 1 cut(s) 1129
HindIII AAGCTT 1 cut(s) 1246
HpaII CCGG 3 cut(s) 96, 833, 1706
HphI GGTGA 3 cut(s) 45, 452, 1247
Hpy166II GTNNAC 3 cut(s) 1129, 1358, 1433
Hpy188III TCNNGA 5 cut(s) 14, 456, 527, 1181, 1283
Hpy8I GTNNAC 3 cut(s) 1129, 1358, 1433
HpyAV CCTTC 7 cut(s) 297, 340, 439, 609, 652, 964, 1063
HpyCH4III ACNGT 8 cut(s) 453, 722, 759, 1077, 1120, 1201, 1414, 1630
HpyCH4IV ACGT 2 cut(s) 978, 1747
HpyF10VI GCNNNNNNNGC 4 cut(s) 505, 811, 1741, 1750
HpyF3I CTNAG 3 cut(s) 495, 1402, 1740
HpySE526I ACGT 2 cut(s) 978, 1747
Hsp92II CATG 8 cut(s) 135, 780, 809, 1214, 1235, 1298, 1421, 1499
HspAI GCGC 1 cut(s) 1735
Ksp22I TGATCA 1 cut(s) 1716
Kzo9I GATC 3 cut(s) 102, 1279, 1716
LguI GCTCTTC 1 cut(s) 223
LmnI GCTCC 3 cut(s) 520, 546, 1472
Lsp1109I GCAGC 4 cut(s) 225, 520, 826, 1789
LweI GCATC 1 cut(s) 1484
MaeII ACGT 2 cut(s) 978, 1747
MaeIII GTNAC 4 cut(s) 473, 779, 796, 995
MalI GATC 3 cut(s) 104, 1281, 1718
MboI GATC 3 cut(s) 102, 1279, 1716
MhlI GDGCHC 1 cut(s) 1403
MlyI GAGTC 4 cut(s) 174, 290, 602, 917
MmeI TCCRAC 1 cut(s) 142
MroXI GAANNNNTTC 1 cut(s) 555
MseI TTAA 6 cut(s) 236, 1308, 1457, 1467, 1662, 1760
MspA1I CMGCKG 1 cut(s) 926
MspI CCGG 3 cut(s) 96, 833, 1706
MspR9I CCNGG 3 cut(s) 20, 519, 834
MvaI CCWGG 2 cut(s) 20, 519
MwoI GCNNNNNNNGC 4 cut(s) 505, 811, 1741, 1750
NciI CCSGG 1 cut(s) 834
NcoI CCATGG 1 cut(s) 1294
NdeI CATATG 1 cut(s) 1352
NdeII GATC 3 cut(s) 102, 1279, 1716
NlaIII CATG 8 cut(s) 135, 780, 809, 1214, 1235, 1298, 1421, 1499
NlaIV GGNNCC 4 cut(s) 831, 837, 1300, 1384
NmuCI GTSAC 2 cut(s) 473, 779
NspI RCATGY 1 cut(s) 780
PaqCI CACCTGC 1 cut(s) 64
PciI ACATGT 1 cut(s) 776
PciSI GCTCTTC 1 cut(s) 223
PdmI GAANNNNTTC 1 cut(s) 555
PflMI CCANNNNNTGG 1 cut(s) 1139
PkrI GCNGC 4 cut(s) 215, 510, 816, 1779
PleI GAGTC 4 cut(s) 173, 289, 601, 916
PpsI GAGTC 4 cut(s) 173, 289, 601, 916
PpuMI RGGWCCY 2 cut(s) 521, 836
PscI ACATGT 1 cut(s) 776
Psp124BI GAGCTC 1 cut(s) 1403
Psp5II RGGWCCY 2 cut(s) 521, 836
Psp6I CCWGG 2 cut(s) 18, 517
PspGI CCWGG 2 cut(s) 18, 517
PspN4I GGNNCC 4 cut(s) 831, 837, 1300, 1384
PspPI GGNCC 3 cut(s) 521, 836, 1414
PspPPI RGGWCCY 2 cut(s) 521, 836
PsrI GAACNNNNNNTAC 2 cut(s) 813, 845
PstI CTGCAG 1 cut(s) 1612
PstNI CAGNNNCTG 2 cut(s) 570, 885
PvuII CAGCTG 1 cut(s) 926
RsaI GTAC 4 cut(s) 409, 1074, 1117, 1434
RsaNI GTAC 4 cut(s) 408, 1073, 1116, 1433
SacI GAGCTC 1 cut(s) 1403
SapI GCTCTTC 1 cut(s) 223
SaqAI TTAA 6 cut(s) 236, 1308, 1457, 1467, 1662, 1760
SatI GCNGC 4 cut(s) 214, 509, 815, 1778
Sau3AI GATC 3 cut(s) 102, 1279, 1716
Sau96I GGNCC 3 cut(s) 521, 836, 1414
SchI GAGTC 4 cut(s) 174, 290, 602, 917
ScrFI CCNGG 3 cut(s) 20, 519, 834
SduI GDGCHC 1 cut(s) 1403
SfaNI GCATC 1 cut(s) 1484
SfcI CTRYAG 1 cut(s) 1608
SinI GGWCC 3 cut(s) 521, 836, 1414
SmlI CTYRAG 5 cut(s) 34, 265, 577, 892, 1787
SmoI CTYRAG 5 cut(s) 34, 265, 577, 892, 1787
SsiI CCGC 3 cut(s) 43, 184, 743
SstI GAGCTC 1 cut(s) 1403
StyD4I CCNGG 3 cut(s) 18, 517, 832
StyI CCWWGG 2 cut(s) 603, 1294
TaaI ACNGT 8 cut(s) 453, 722, 759, 1077, 1120, 1201, 1414, 1630
TaiI ACGT 2 cut(s) 981, 1750
TaqI TCGA 1 cut(s) 1316
TatI WGTACW 3 cut(s) 407, 1115, 1432
Tru1I TTAA 6 cut(s) 236, 1308, 1457, 1467, 1662, 1760
Tru9I TTAA 6 cut(s) 236, 1308, 1457, 1467, 1662, 1760
TscAI CASTG 5 cut(s) 424, 727, 1144, 1206, 1726
TseFI GTSAC 2 cut(s) 473, 779
TseI GCWGC 4 cut(s) 213, 508, 814, 1777
Tsp45I GTSAC 2 cut(s) 473, 779
TspGWI ACGGA 1 cut(s) 1023
TspRI CASTG 5 cut(s) 424, 727, 1144, 1206, 1726
Van91I CCANNNNNTGG 1 cut(s) 1139
VpaK11BI GGWCC 3 cut(s) 521, 836, 1414
XagI CCTNNNNNAGG 1 cut(s) 516
XapI RAATTY 8 cut(s) 64, 86, 338, 400, 1184, 1568, 1690, 1756
XceI RCATGY 1 cut(s) 780
XmnI GAANNNNTTC 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.